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1.
采用对羟基联苯比色法对35只青海土种山羊血乳酸于采血后第2天和第8天进行测定,结果:被检土种山羊血乳酸值为2.38±1.62m mol/L,而且在性别、年龄组间无显著差异;血样保存2天和8天时间对血乳酸值也无显著影响。 相似文献
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扬中市地处长江之中,四面环江,湿度较大。我市农户饲养山羊历史较长,近几年来养羊专业户逐渐增多,山羊寄生虫病的危害随之显现出来。2007年6月份笔者应邀出诊,经临床观察结合剖检及粪检诊治一例山羊血矛线虫病,经用左旋眯唑对羊群治疗,有效地控制了该病。 相似文献
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采用淋巴细胞杂交瘤技术,研制出的抗衣原体单克隆抗体,应用酶联免疫吸附试验(ELISA)方法,对本地区所采用的698份羊血清进行检测,羊血清阳性检出率为22.06%,选择50份羊血清用已建立的检测衣原体抗体ELISA方法与传统的间接血凝试验(IHA)进行比较,其敏感性高。羊血清ELISA阳性检出率为26%,IHA为22%,前者比IHA高出4个百分点。 相似文献
4.
随着免疫化学诊断药盒在临床医学上的广泛应用,其药盒中重要试剂之一——第二抗体的需求量不断增加。近年来,多用马和驴等大动物为免疫动物,以限量多次采血法生产大量第二抗体血清。我们在研制出口用马抗羊血清过程中,观察到马抗羊血清与传贫琼扩抗原之间可发生非特异性反应。通过试验,明确了该反应是由传贫琼扩抗原中被浓缩的牛血清同马抗羊血清抗体之间交叉所致的,现报道如下。 相似文献
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采用氟离子选择电极法。对50只藏羊血清氟含量进行测定。结果表明,玉树地区藏羊血清氟含量为0.181-0.594μg/ml,证明该方法适用于藏羊血清氟含量的测定。 相似文献
7.
衣原体单克隆抗体在牛羊血清诊断上的应用 总被引:1,自引:0,他引:1
采用淋巴细胞亲交瘤技术,研制出的抗衣原体单克隆抗体,应用酶联免疫吸附试验(ELISA)方法,对本地区所采集的128份羊血清、90份牛血清进行检测,结果,羊血清阳性检出率为25.8%,牛血清阳性检出率为32.2%,选择50份羊血清、50份牛血清用已建立的检测衣原体抗体ELISA与间接血凝试验(IHA)进行比较,牛血清ELISA阳性检出率为32.0%,IHA为24.0%,ELISA比IHA试验高出8个百分点;羊血清ELISA阳性检出率为26.0%,IHA为22.0%,ELISA比IHA高出4个百分点。 相似文献
8.
赵金全 《青海畜牧兽医杂志》2004,34(2):43-43
自1995年12月开始,每年冬末春初,祁连县绵羊群不同程度感染羊血虱,采用多种药物预防和治疗效果欠佳,经应用高浓度敌百虫溶液对羊血虱的防治效果比较满意。 相似文献
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山羊痘病毒粒子的电子显微镜观察 总被引:12,自引:1,他引:12
本研究室2003年以来对山羊痘病毒感染多种细胞进行了透射电镜观察。在感染的皮肤和黏膜上皮细胞浆中观察到大量不同成熟阶段的典型山羊痘病毒粒子,病毒粒子也见于巨噬细胞、成纤维细胞、血管内皮细胞胞浆内,甚至少量散在血管腔中。用山羊痘病毒分离物感染鸡胚绒毛尿囊膜形成痘斑,其上皮细胞和成纤维细胞中也可见成熟和不成熟的病毒粒子,同时感染BHK-21细胞也观察到典型的山羊痘病毒粒子。 相似文献
11.
双峰驼血淋巴结的形态结构研究 总被引:1,自引:1,他引:0
本研究用解剖学和组织学方法首次确定我国双峰驼具有血淋巴结这一形态结构。其外观呈卵圆形、紫红色,直径6-11mm,常见于皮下结缔组织中。在组织学上,外有一层由胶原纤维、弹性纤维和成纤维细胞构成的薄而疏松的被膜,其中有较多的血管和淋巴管;被膜伸入内部形成细小且分布散乱的小梁,但未将实质分隔成明显的小叶。实质由大量的血窦、较少的淋巴小结和淋巴窦构成,未见索状淋巴组织。血窦可分为边缘窦和中间窦,充满血液,前者位于被膜下方,较宽大,呈索状排列;后者狭窄,吻合成网;窦壁由内皮和基膜组成。淋巴窦较少,窦壁完整,明显扩张。淋巴小结多呈卵圆形,散在分布于血窦之间,由淋巴、网状组织构成,有的有明显的的生发中心。和他种动物相比,双峰驼血淋巴结在组织学上有以下特点:(1)除具有血管和血窦外,还有淋巴管和淋巴窦;(2)实质无皮质和髓质之分;(3)淋巴组织主要为淋巴小结,未见索状的淋巴组织。 相似文献
12.
In this study, the presence of haemal nodes, previously recognised especially in ruminants, was determined for the first time in piglets. The morphology of haemal nodes located in the abdominal cavity of seven piglets was studied by gross inspection and by histological methods. Haemal nodes reddish in colour and oval or round in shape were observed on the thin branches originating from the caudal and cranial mesenteric arteries. These nodes, having connection only with blood vessels, were covered with a thin connective tissue capsule. The haemal nodes had a hilus at the entrance of which large blood vessels were seen. These blood vessels extended into the inside of the nodes through trabeculae. Afferent and efferent lymph vessels were not observed in the haemal nodes. The nodes contained many sinuses formed by reticular fibres and reticular cells and filled by red blood cells. In the haemal nodes, lymphocytic infiltration was more commonly seen than lymph follicles. 相似文献
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P. L. Papadopoulou M. N. Patsikas A. Charitanti G. M. Kazakos L. G. Papazoglou M. Karayannopoulou I. Chrisogonidis N. Tziris A. Dimitriadis 《Anatomia, histologia, embryologia》2009,38(4):292-299
Seventy-three clinically normal, lactating cats were used to investigate the lymph drainage of 73 mammary glands. In 50 cats of the first group, the number of lymphatic vessels emerging from the examined mammary gland, their course and the lymph nodes into which they are drained were studied by indirect lymphography (IL) after intramammary injection of an oily contrast medium. In 23 cats of the second group, the lymph drainage of the mammary glands was studied by computerized tomography indirect lymphography (CT-IL) after intramammary injection of a water soluble contrast medium. The following day, the lymph drainage of the mammary gland examined by CT-IL was studied by IL, as it was described in the first group, for comparison purposes. The main conclusions drawn after this study were as follows: lymph drains from the first and second mammary glands with one or rarely two or three lymphatic vessels to the accessory axillary lymph nodes. Lymph drains from the third mammary gland with one or two and rarely three lymphatic vessels usually to the accessory inguinal lymph nodes or to the accessory axillary lymph nodes. In some cases, it drains to both lymph nodes simultaneously or it may rarely drain only to the medial iliac lymph nodes. The fourth mammary gland with one or two and rarely three lymphatic vessels usually drains to the accessory inguinal lymph nodes. It may rarely drain only to the medial iliac lymph nodes. Mammary lymphatic vessels that cross the midline and lymphatic connection between the mammary glands were not demonstrated. No differences in the mammary lymph drainage pattern between IL and CT-IL were found. 相似文献
14.
Rochelle M. Salwei Robert T. O''Brien Jodi S. Matheson 《Veterinary radiology & ultrasound》2005,46(5):411-416
Doppler ultrasound has been used in humans to determine angioarchitecture of lymph nodes as the criterion for the determination of malignancy. We hypothesized that the vascular and perfusion patterns of a canine malignant lymph node could be characterized with intravenous microbubble ultrasound contrast media and that contrast harmonic ultrasound could provide better conspicuity of the angioarchitecture when compared with Power Doppler ultrasound. In this study, 11 peripheral lymph nodes in dogs with histologically verified malignant lymphoma were imaged with fundamental ultrasound, Power Doppler ultrasound, and three contrast harmonic pulse sequences to characterize the vascular pattern and perfusion. Vascular imaging was greatly enhanced in these nodes with 2.13 times more vessels seen with contrast harmonic ultrasound compared with Power Doppler ultrasound (P < 0.01). The angioarchitecture of lymphomatous lymph nodes of dogs in this study were similar to those previously described in malignant superficial lymph nodes in human patients; 45.5% of the nodes had displacement of the central hilar vessel, 45.5% had aberrant vessels, 63.6% had pericapsular vessels, 36.4% had subcapsular vessels, and 81.8% had loss of the central hyperechoic band in fundamental sonography. Poor perfusion, indicated by a lower mean pixel intensity increase between pre- and postcontrast administration images, was seen in 36.4% of the lymph nodes while 63.6% had fair to good perfusion. The perfusion patterns in nine of the 11 lymph nodes were homogenous and two showed focal hypoperfused regions. We conclude that Power Doppler and contrast harmonic ultrasound are beneficial in accurately depicting angioarchitechture and can provide additional information in determining the presence of malignant vascular characteristics within lymphomatous nodes in dogs. 相似文献
15.
In sheep, most blood vessels enter the lymph nodes at a discrete hilus, which is where the capsule overlies medullary tissue. In pigs, vessels within the node arise from an extensive network of arteries on the surface, and most vessels penetrate the capsule where it overlies diffuse tissue--the counterpart of the sheep medulla. In both species the blood vessels divide extensively as they approach and supply the dense lymphoid tissue which contains lymph nodules. The blood vessels within sheep lymph nodules are surrounded by a thick layer of amorphous material. The dense lymphoid tissue has an abundant network of venules which have smooth muscle cells in their walls. These venules are involved in lymphocyte migration and in pigs they are lined by high endothelium which is similar to that in most other species, while in sheep the endothelium is much lower. Fenestrated capillaries occur in both pig and sheep lymph nodes. 相似文献
16.
The lymph nodes of the stomachs taken from 31 sheep and 35 goats were described and measured. Compared with lymph nodes of cattle (Baum, 1912) marked differences were found regarding the position, number and presence of the nodes, as well as in regard to the lymphatic vessels leaving them. The results are discussed in the light of the decisions that may have to be made during meat inspection. 相似文献
17.
The distribution and characteristics of lymph vessels in caprine hemal nodes were studied after glutaraldehyde fixation and epoxy resin embedding. Histologically, the lymph vessels were characterized by thin walls and wide lumens containing inspissated lymph in which a few cells were suspended. The lymph vessels contrasted sharply with adjacent blood sinuses that were filled with elements of circulating blood. A circumferential lymph vessel in the cortex joined radial branches in the medulla that met at the hilum to drain through a large efferent lymph channel. Ultrastructurally, the lymph vessel wall comprised endothelial cells supported by a continuous basal lamina, collagen fibrils, and adventitial reticular cells. The cytoplasm of endothelial cells had fenestrations, plasmalemma-associated vesicles, vacuoles, and focal splits that enclosed large compartments. Many compartments contained erythrocytes and lymphocytes. Modifications of the endothelial cells signified their endowment with features that favored transendothelial transport. The distribution of lymph vessels and the finding of only efferent lymph channels are related to the roles of hemal nodes in blood storage by hemoconcentration and in immune defense mechanisms. 相似文献
18.
A REVIEW OF SONOGRAPHIC IDENTIFICATION OF ABDOMINAL BLOOD VESSELS AND JUXTAVASCULAR ORGANS 总被引:4,自引:1,他引:3
Abdominal vasculature can be evaluated non-invasively using 2-D ultrasound imaging and Doppler ultrasonography.5,6,12 The identification of abdominal vessels using ultrasound is based on knowledge of their normal location, appearance and relationship to specific organs. Because anatomic location of major abdominal vessels is fairly consistent, finding and following vessels is a useful aid when attempting to locate and evaluate juxta-vascular organs. Sonographic vascular evaluation may be beneficial in providing information regarding central cardiovascular abnormalities, detection of abnormalities involving the vessel interrogated, or detection of abnormalities of the parenchymal bed that the vessel supplies,5–12 Knowledge of the location and appearance of the vessels is necessary for this information to be of diagnostic value.
In this paper, abdominal vessels in the dog are reviewed and a sonographic map of major abdominal vessels is described. This includes the sonographic appearance of the aorta, caudal vena cava, and portal vein, their major branches and their anatomic relationship with adjacent organs.
Identification of the medial iliac lymph nodes, mesenteric lymph nodes, abdominal esophagus, duodenum, spleen, kidneys, pancreas, adrenal glands and liver lobes are assisted by recognizing specific vascular landmarks. The locations and appearances of pertinent organs as pertains to abdominal vasculature (caudal vena cava and aorta) are described. 相似文献
In this paper, abdominal vessels in the dog are reviewed and a sonographic map of major abdominal vessels is described. This includes the sonographic appearance of the aorta, caudal vena cava, and portal vein, their major branches and their anatomic relationship with adjacent organs.
Identification of the medial iliac lymph nodes, mesenteric lymph nodes, abdominal esophagus, duodenum, spleen, kidneys, pancreas, adrenal glands and liver lobes are assisted by recognizing specific vascular landmarks. The locations and appearances of pertinent organs as pertains to abdominal vasculature (caudal vena cava and aorta) are described. 相似文献
19.
研究了咪唑类化合物KK—42对家蚕卵巢发育以及蛋白质合成的影响,结果表明KK-42对卵巢增重有明显的抑制作用,血淋巴蛋白含量在蛹期变化幅度很小,卵巢蛋白质含量增加也很缓慢;而对照区在化蛹后,血淋巴蛋白急剧增加,第三天达到高峰,以后又迅速下降,与卵巢蛋白质增加相吻合。由此表明,咪唑类化合物KK-42不仅抑制血淋巴蛋质的合成,同时还抑制了血淋巴蛋白向卵巢的转移。 相似文献
20.
Nyman HT Lee MH McEvoy FJ Nielsen OL Martinussen T Kristensen AT 《American journal of veterinary research》2006,67(6):978-984
OBJECTIVE: To compare and correlate B-mode and color Doppler ultrasonographic characteristics with histopathologic findings of benign and malignant superficial lymph nodes in dogs. STUDY POPULATION: 50 superficial lymph nodes that were normal, abnormally large on physical examination, or represented regional lymph nodes draining an area of suspected primary malignancy in 30 dogs. PROCEDURES: Before excision, lymph nodes were evaluated via B-mode and color Doppler ultrasonography to assess size, echogenicity, presence of a hilus, acoustic transmission, and vascular flow. Formalin-fixed, paraffin-embedded tissue sections of excised lymph nodes were stained with H&E and examined for the presence and extent of necrosis, fibrosis, fat, metastases, and tissue heterogeneity. To assess vascularity, the number and distribution of vessels stained by the Verhoeff van Gieson technique were recorded. RESULTS: In superficial lymph nodes, a varied echogenicity corresponded to tissue heterogeneity. The ultrasonographic detection of a hilus was associated with the presence of fibrous tissue, fat, or both in the hilar region. Acoustic enhancement corresponded to presence of areas of intranodal necrosis. There was significant correlation between both the distribution and the number of vessels detected via ultrasonography and that detected by histopathology. The amount of flow estimated via ultrasonography was typically higher than that estimated via histologic examination. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that histopathologic changes in canine lymph nodes have associated ultrasonographic changes and suggest that lymph node ultrasonography has an important role in the evaluation of lymph nodes in dogs in general and in dogs with neoplastic disease in particular. 相似文献