2. Treatments consisted of two cereals (maize- or wheat-based diets) and three levels of SKEO)0, 250 and 500 mg/kg).
3. Feed intake (FI) and body weight gain (BWG) decreased in broilers fed on the wheat-based diets compared to the control diet. Dietary supplementation of 500 mg/kg SKEO increased FI and BWG. Dietary SKEO supplementation was effective in improving FI and BWG only in wheat-based diets and was effective in improving feed conversion ratio (FCR) in both diets.
4. Dietary supplementation of 500 mg/kg SKEO caused a significant decrease in plasma cholesterol and triglycerides. Also, plasma low-density lipoprotein concentration was decreased in broilers fed on 250 and 500 mg/kg of SKEO. Plasma cholesterol levels in birds fed on the wheat-based diets were lower than in those fed on maize-based diets.
5. Dietary supplementation of 500 mg/kg SKEO increased caecal population of Lactobacillus and reduced total bacterial and Escherichia coli count. Caecal population of Lactobacillus decreased in broilers fed on the wheat-based diets.
6. Digesta viscosity was increased in broilers fed on the wheat-based diets and decreased in broilers fed on 500 mg/kg SKEO compared to the control diet. Dietary SKEO supplementation was effective in reducing digesta viscosity values only in wheat-based diets.
7. Villus height of the duodenum and jejunum decreased in broilers fed on the wheat-based diets. Dietary supplementation of 500 mg/kg SKEO increased villus height and villus height-to-crypt depth ratios and decreased crypt depth of the duodenum compared to the control diet.
8. It can be concluded that dietary SKEO supplementation was effective in improving FI, BWG and FCR values in wheat-based diets. 相似文献
2. Broilers were subjected to 1 of 3 handling methods for 2 min: (i) 3 birds held in an inverted position in one hand (multiple inverted, MI), (ii) 1 bird held in an inverted position (single inverted, SI), or (iii) upright handling (U), which consisted of holding a bird gently with both hands in an upright position.
3. In experiment 1 birds were returned to their respective pens after handling and in experiment 2 they were crated for up to 4 h after the imposition of handling. In experiment 2 food deprived (FD) and fully fed (FF) uncrated birds were also sampled as controls. Blood samples were collected immediately after handling and at 1‐h intervals after handling or handling and crating.
4. In experiment 1, MI and SI birds had higher CS concentrations than U birds.
5. In experiment 2, there were no significant differences in CS concentrations of crated birds despite the different handling treatments. FD birds had higher mean CS concentrations than FF birds, although not as high as those of crated birds. CS concentrations peaked at 3 h after crating. Birds showed predominantly resting behaviour after crating.
6. These results suggest that handling stress can be reduced in broilers by the use of upright handling. However, the methods of handling is not important in influencing CS concentrations if the birds are crated after handling, either because crating is a more potent stressor than handling or due to stressor additivity. 相似文献
2. The study was conducted to determine the tetracycline resistance and prevalence of tetracycline resistance determinants among Escherichia coli strains isolated from broilers in northern Iran.
3. Minimum inhibitory concentration (MIC) of tetracycline and susceptibility pattern of the isolates were screened using micro-dilution and disk diffusion methods, respectively. The presence of 7 tetracycline resistance genes including tetA, tetB, tetC, tetD, tetE, tetG and tetM was tested using the polymerase chain reaction.
4. Among 100 strains isolated from broilers, 73% were identified as tetracycline resistant. All isolates showed the presence of tetracycline-associated genes. The most prevalent genes were tetA (46%) and tetB (41%) and totally, 17 different genotypes were recognised according to the presence of tetracycline resistance genes. Statistical analysis revealed that concomitant presence of the resistance genes significantly increased the tetracycline MIC and effectiveness of phenotypic characterisation.
5. The results demonstrated a high occurrence of tetracycline-resistant E. coli and related genes among broilers which presents a risk of increasing these strains in human infections associated with food animals. 相似文献
2. Growth was significantly enhanced by regular handling in broilers and the females of the layer strains and gain : food ratios were generally greater in the handled birds.
3. There were no significant treatment effects on growth or gain : food ratios in males of the layer strains.
4. Males had higher relative weight gains and gain : food ratios than females. 相似文献
2. A total of 160 chickens from slow- and fast-growing broilers were raised under same conditions. Half of the broilers from each strain were encouraged to wing flap when they reached 2800 g body weight. Pectoralis minor (p. minor) muscle of the broilers was inspected for the occurrence of DPM and p. major samples were collected from broilers with or without DPM. The muscle fibre area and number, capillary number and the signalling pathways of vascular development (vascular endothelial growth factor A, VEGFA) and muscle contraction regulation (actin alpha 1, ACTA1; myosin light chain kinase 2, MYLK2 and ATPase Ca+2 transporting gene 1, ATP2A1) were studied in p. major muscle.
3. DPM induction increased fibre area of p. major muscle with a greater rate in the slow-growing strain compared with fast-growing line. Although the capillary number was higher in slow-growing compared with fast-growing broilers, in the case of DPM induction, the number of capillaries was similar between strains.
4. Expression of VEGFA, MYLK2 and ATP2A1 was greater in slow- than in fast-growing broilers. DPM induction increased expression of ACTA1, VEGFA and ATP2A1 in p. major muscle of broilers from both strains; however, MYLK2 expression was downregulated.
5. Changes in capillary density and expression of VEGFA found in the p. major muscle of broilers with DPM suggest increased blood flow to increase oxygen availability. The upregulation of ATP2A1 by DPM induction could be attributable to alterations in calcium ion transportation from the cytoplasm into the sarcoplasmic reticulum.
6. The results are evidence of changes in muscle structure and gene expression pathways in p. major muscle of broilers with DPM. 相似文献
2. Meat quality variables studied included pH of the pectoralis major muscle measured at 20 min post‐mortem and at filleting, and texture at 24 h post‐mortem.
3. Argon stunning resulted in a rapid early post‐mortem pH fall, but there was no indication of heat toughening in the breast muscles.
4. Filleting at 2 h post‐mortem, after argon stunning, resulted in 5% of the p. major muscles having greater than 4 kg yield force (slightly tough).
5. Although post‐mortem ageing of argon stunned broilers improved the texture of breast muscle, it is inferred that filleting at 2 h postmortem resulted in breast muscle with acceptable texture. 相似文献
2. Dietary supplementation of FPB linearly increased the weight gain and feed intake of broilers with linear reduction in feed conversion ratio.
3. The crude protein, iron, magnesium, and sodium content were linearly higher, whereas cholesterol was linearly lower in the breast meat of FPB-supplemented broilers. In thigh meat, linearly lower ether extract and cholesterol with higher moisture was noted in response to increasing levels of FPB.
4. The proportion of saturated fatty acids was both linearly and quadratically lower in breast and thigh meat, whereas those of monounsaturated fatty acids of breast (linear and quadratic) and n-3 fatty acids of breast and thigh (linear) meat was higher in the FPB-supplemented broilers. The n-6/n-3 ratio of breast meat was linearly lower in response to FPB supplementation. The hypocholesterolaemic to hypercholesterolaemic ratio of thigh meat was higher in the FPB-supplemented groups.
5. The thiobarbituric acid reactive substances and pH value were lower in the breast and thigh meat of FPB-supplemented broilers.
6. Thus, additive supplementation of the diet with up to 2% FPB improved the nutritional quality, fatty acid profile and shelf life of broiler meat. 相似文献
2. Compared with electrical stunning, killing broilers with the gas mixture eliminated or substantially reduced the prevalence of carcase and meat quality defects.
3. The results also showed that killing broilers with a mixture of 30% carbon dioxide and 60% argon would enable filleting (deboning) to be performed at 4 h post mortem without adversely affecting the cook loss or texture of breast meat. 相似文献
2. To simulate the external (dust and feather) and internal (intestinal) contamination of broilers the carcasses were artificially contaminated with a strain of Escherichia coli K12.
3. Cross‐contamination occurred at both stages in the processing when the carcasses had been contaminated externally; when the broilers had been contaminated internally slight cross‐contamination occurred only during plucking.
4. Broilers which were contaminated externally before scalding resulted in more numerous carcasses that were contaminated after the whole slaughtering procedure than those contaminated internally.
5. In one processing plant there were fewer contaminated carcasses after cooling than after plucking, while in the other plant no differences were found in the number of positive carcasses after these two stages in processing. 相似文献
2. Free range (FR) and standard (STD) broilers were killed and processed under commercial conditions and the M. pectoralis major muscle assessed for ultimate pH (pHu), sarcomere length, cooking loss and texture 24 h and 7 d after slaughter.
3. Although FR broilers were females with an average age of 60 d, whereas the STD broilers were 45 d old and predominantly male, there were no significant differences in mean values obtained for pHu, sarcomere length, cooking loss and shear force results between the two bird types.
4. While aging for 7 d increased the pHu and cooking loss means, sarcomere length means remained unchanged and shear force values decreased by 6% and 9% for FR and STD birds respectively.
5. Variability in shear force values also decreased on aging for 7 d, no birds having values above 4 kg/cm2 on day 7 whereas 16% were above this value on day 1.
6. There were significant correlations between the results for shear force, pHu and sarcomere length, indicating that increased shear force values were associated with a decrease in both pHu and sarcomere length.
7. These correlations suggest that variability in the texture of cooked M. pectoralis major from FR and STD chickens is strongly influenced by those post‐mortem biochemical changes which lead to the development of rigor during the early stages of processing. 相似文献
2. In Experiment 1, a total of 116 250 carcasses from 59 Ross-308 broiler flocks, classified according to slaughter weight as 2.0–2.2, 2.2–2.4, 2.4–2.6 and >2.6 kg, were evaluated for occurrence of DPM. In Experiment 2, p. major samples from unaffected broilers and each DPM stage were evaluated for meat quality, oxidant and antioxidant properties, nutritional value and fatty acid profile. DPM was characterised as 1: muscles with coagulative necrosis, 2: muscles with fibrous tissue texture and pink to plumb and 3: muscles with green necrotic area.
3. The average incidence of DPM was found to be 0.73% in Experiment 1 and independent of slaughter weight.
4. In Experiment 2, p. major muscle of broilers with DPM 1 and 2 had higher pH values with higher redness and drip loss. All DPM stages resulted in an increase in lipid content and malondialdehyde activity and lowered ash content of p. major muscle compared with unaffected birds. DPM 2 increased superoxide dismutase and glutathione peroxidase activities in M. p. major. The p. major of broilers with DPM had lower content of C18:2 conjugated linoleic and C20:3n-6 fatty acids than those of unaffected broilers. Lower Δ6 desaturase and thiosterase activities and 18:2n-6 to 18:3n-3 ratio were observed for all DPM stages compared to unaffected.
5. It was concluded that these changes obtained in p. major muscle of broilers with DPM might indicate biochemical characteristics of muscle degenerations. 相似文献
2. Two replicates of broilers (age 54 and 59 d respectively) were stunned in carbon dioxide, in argon or by electric current, and the carcases were plucked either by a mechanical plucker or by hand.
3. Carcase and meat quality were evaluated to determine the effect of these stunning treatments and plucking methods. The quality factors measured included pH at 20 min post‐mortem, ultimate pH, texture and colour of the pectoralis major muscle, and the incidence of carcase appearance defects, cooking loss, broken bones and bruises in the breast and leg muscles.
4. Stunning method had a significant effect on pH at 20 min, ultimate pH, texture and colour of breast meat. Plucking method had a significant effect on the cooking loss and texture.
5. Argon stunning resulted in fewer muscle haemorrhages, a more rapid early post‐mortem fall in pH and more tender breast meat than in the electrically stunned broilers; carbon dioxide stunned broilers had intermediate values for pH decline and meat tenderness but were not significantly different from argon stunning regarding muscle haemorrhages.
6. Appearance defects occurred to a similar extent in all the stunning methods and mechanical plucking resulted in 4 times as many defects compared with hand plucking; the incidence of broken bones was higher in mechanically plucked broilers than in hand plucked carcases.
7. Gaseous stunning of broilers produced relatively better quality carcases and meat than electrical stunning, and therefore may have commercial advantages. 相似文献
2. Myofibres were divided into Types II‐R, II‐I and II‐W showing high, moderate and low reduced nicotinamide adenine dinucleotide dehydrogenase (NADH‐DH) activities, respectively.
3. Subsarcolemmal formazan granules indicating very strong NADH‐DH activity in Type II‐R fibres were observed only in KC roasters. In both sexes the percentage of Type II‐R fibres present was greater in KC roasters than in broilers.
4. These results indicated marked differences in histochemical properties and meat quality between KC roasters and broilers. 相似文献
2. The incidence of twisted legs was influenced by strain and for males was twice that for females. There was also a higher incidence in cages than on litter with the type of cage floor having an effect: broilers reared on floors of metal wire and perforated sheets had more leg problems than those reared on plastic mats and plastic‐covered wire.
3. Although vitamin and mineral supplementation had no effect on caged broilers, a restriction of metabolisable energy (ME) intake during the first 14 d after hatching halved the frequency of leg abnormalities compared with those fed ad libitum.
4. Studies of cage size and location of water suggested that a lack of exercise increases the incidence of leg abnormalities. 相似文献
2. Results showed a significant difference on the anticoccidial efficacy 6 d after treating infected animals with Baycox (Et: 99.69% and Ea: 99.52%) or gen-TTZ (Et:85.71% and Ea 81.81%).
3. Gen-TTZ-treated animals were less strongly carotenoid-pigmented than Baycox-treated broilers. Mean plasma carotenoid concentrations were significantly higher in groups treated with Baycox than in broilers given gen-TTZ.
4. Treatment of animals with Baycox led to a significant decrease in ability of the peripheral blood mononuclear cells to produce ROS in contrast to gen-TTZ-treated groups. Baycox, but not generic toltrazuril, increased IL-10 and decreased tumour necrosis factor alpha (TNF-α) concentrations in chickens infected with E. tenella and E. acervulina.
5. It is suggested that differences in anticoccidial efficacy may be observed when using a generic toltrazuril product. Hence, in addition to plasma profiles of drugs, standardised clinical control tests may be necessary for generic formulations, particularly if other parameters are important to achieve a better control of coccidiosis. 相似文献
2. The low AME was due to a reduced starch digestibility and was not improved by grinding the wheat finely or by steam pelleting the diet.
3. Higher AME values were obtained when determinations were made with adult cockerels than with growing broilers when the conventional technique was used.
4. AME values determined using a rapid bioassay technique (Farrell, 1978) with growing broilers or adult cockerels were considerably higher than values determined using the conventional procedure with growing broilers.
5. Inter‐laboratory studies showed that the true metabolisable energy (TME) assay gave reproducible values for individual and blended wheat samples, but that these values were higher than AME values determined by the conventional procedure. Similar TME values were found with growing broilers and adult cockerels. 相似文献
2. Viscosity of TBS was noticeably increased in the ambroxol‐treated birds and only slightly increased in the bromhexine groups; however, the physical (nature) of TBS was superior in the ambroxol‐treated broilers.
3. There was a clear increase in the passage of furaltadone into tracheobronchial secretions only in the ambroxol‐treated birds.
4. Everyday use of ambroxol in broilers is discussed. 相似文献
2. Three groups of broilers (60 each) were fed on a 3% flaxseed oil (high α-linolenic acid (ALA)) diet for either 6, 4 or 2 weeks prior to slaughter. During the remaining time they were maintained on a 3% macadamia oil (low ALA) diet. A fourth group (control, n = 60) was fed on a commercial diet for 6 weeks.
3. No significant difference was observed in growth performance of broilers between groups. The amounts of total n-3 and n-3 LCPUFA in breast and thigh meat were not different between broilers fed the flaxseed oil diet for 4 and 6 weeks, but they were lower (P < 0.001) in those fed the flaxseed diet for only 2 weeks.
4. These results suggest comparable levels of n-3 LCPUFA in the meat can be achieved by only feeding the flaxseed oil diet in the last 3–4 weeks of the growth period; this would result in a ≥ 9.4% reduction in the use of flaxseed oil compared to 6 weeks of feeding; thereby reducing the cost of the enrichment process. 相似文献
2.?A total of 720 male Ross broilers were divided into two dietary treatments with 12 replicate pens per treatment. Broilers were given a control soybean-wheat-based diet with or without added EO in two diet phases (0–21 d and 22–42 d).
3.?The blend of EO increased body weight gain of broilers from 0 to 42 d by 4?5%.
4.?Caecal microbiota were affected by the EO blend; in particular increases in the proportions of Lactobacillus and Escherichia coli at 41 d was observed.
5.?The EO blend had major effects on caecal metabolites. The proportion of caecal butyrate at 20 and 41 d of age increased, whereas the proportion of caecal acetic acid at 20 d, and propionic acid and isovaleric acid at 41 d, decreased with the EO blend. In addition, the caecal proportion of spermine increased and tyramine decreased at 41 d of age with the EO treatment.
6.?The present study shows that EO supplementation exerts a positive effect on intestinal microbiota with a concomitant enhancement in growth performance. The study suggests that modulation of broiler gut microbiota composition and activity through the administration of EO offers an effective means for improving broiler performance. 相似文献
2. Broilers selected for food conversion efficiency had greater growth rates and superior food conversion efficiencies than broilers selected for growth rate. No interactions between broiler strain and oxygen level were observed.
3. Increasing environmental oxygen level decreased N efficiency in both broiler strains. Nitrogen turnover was non‐significantly decreased.
4. Ascites may only occur when broilers are able to cope with small environmental adversities while maintaining optimal growth rates. 相似文献