2. Food passage in the crop was significantly reduced by intraperitoneal (IP) injection of LPS while it did not affect the number of defecations, suggesting that LPS may affect food passage only in the upper digestive tract.
3. Similar to LPS, prostaglandin E2 (PGE2), one of the mediators of LPS, also reduced crop-emptying rate in chickens while it had no effect on the number of defecations.
4. Pretreatment with indomethacin, which is an inhibitor of cyclooxygenase (COX), a prostaglandin synthase, had no effect on LPS-induced inhibition of crop emptying.
5. IP injection of LPS did not affect the mRNA expression of COX2 in the upper digestive tract of chickens.
6. It is therefore likely that LPS and PGE2 reduced food passage rate in the crop by a prostaglandin-independent pathway in chickens. 相似文献
2. Imaging was performed two times daily. To localise chickens within the pen, an ellipse fitting algorithm was used and the chickens’ head and tail removed using the Chan–Vese method.
3. The correlations between the body weight and 6 physical extracted features indicated that there were strong correlations between body weight and the 5 features including area, perimeter, convex area, major and minor axis length.
5. According to statistical analysis there was no significant difference between morning and afternoon data over 42 d.
6. In an attempt to improve the accuracy of live weight approximation different ANN techniques, including Bayesian regulation, Levenberg–Marquardt, Scaled conjugate gradient and gradient descent were used. Bayesian regulation with R2 value of 0.98 was the best network for prediction of broiler weight.
7. The accuracy of the machine vision technique was examined and most errors were less than 50 g. 相似文献
2. The objective of this study was to investigate the expression profiles of these genes in skeletal muscles (breast muscle and thigh muscle) at 5 developmental stages (0, 81, 119, 154 and 210 d old) of Tibetan chickens. Relationships between expressions of these genes and growth and carcass traits in these chickens were also estimated.
3. The expression profiles showed that in the breast muscle of both genders the mRNA levels of MRF genes were highest on the day of hatching, then declined significantly from d 0 to d 81, and fluctuated in a certain range from d 81 to d 210. However, the expression of Myf5, Myf6 and MyoG reached peaks in the thigh muscle in 118-d-old females and for MyoD in 154-d-old females, whereas the mRNA amounts of MRF genes in the male thigh muscle were in a narrow range from d 0 to d 210.
4. Correlation analysis suggested that gender had an influence on the relationships of MRF gene expression with growth traits. The RNA levels of MyoD, Myf5 genes in male breast muscle were positively related with several growth traits of Tibetan chickens (P < 0.05). No correlation was found between expressions of MRF genes and carcass traits of the chickens.
5. These results will provide a base for functional studies of MRF genes on growth and development of Tibetan chickens, as well as selective breeding and resource exploration. 相似文献
2. Plasma osmolality, Na+ and K+ concentrations, aldosterone levels, and the expression of aquaporin (AQP) 2, AQP3, and α subunits of the amiloride-sensitive epithelial sodium channel (αENaC) were analysed in the kidneys of 5-week-old MD chickens and White Leghorn (WL) chickens under physiological conditions or after one day of water deprivation.
3. Plasma osmolality, Na+ concentrations, and plasma aldosterone levels were significantly higher in MD chickens than in WL chickens. αENaC mRNA expression levels were lower in MD chickens than in WL chickens. AQP2 and AQP3 mRNA expression levels were similar in the two strains of chickens.
4. Plasma osmolality correlated with aldosterone levels and AQP2 and αENaC mRNA levels in WL chickens. In MD chickens, plasma osmolality correlated with AQP2 mRNA levels, but not with plasma aldosterone or αENaC mRNA levels.
5. These results suggest that neither water reabsorption nor the expression of AQP2 and AQP3 is impaired in MD chickens and that a WWP1 gene mutation may or may not directly induce an abnormality in Na+-reabsorption in the kidneys of MD chickens, potentially through αENaC. 相似文献
2. Storage of uneviscerated Wrolstad turkeys at 4 °C for 10 days caused no statistically significant changes in meat flavour or texture.
3. During further storage at —2 °C, however, there was a slight but significant change in flavour, which became more marked with time in birds which had been eviscerated after the initial period at 4 °C.
4. Both eviscerated and uneviscerated birds became slightly tougher during storage.
5. Initial holding at 4 °C increased the numbers of psychrotrophic bacteria on the skin by about 103 but subsequent changes at — 2 °C were slight for uneviscerated birds.
6. Eviscerated carcases had higher counts than uneviscerated birds after storage at — 2 °C and, although ‘off’ odours were not detected, spoilage appeared to be imminent at the end of the 20‐d period. 相似文献
2. Restricted birds were lighter throughout the experiment.
3. Relative adrenal weight tended to be greater in restricted birds but the difference decreased with time.
4. There was no depletion of adrenal cholesterol: from week 5 there was a significantly greater amount in the adrenals of restricted birds.
5. After 1 week of restriction plasma corticosterone concentration was 73% greater than in controls. It decreased progressively, falling within the normal range at 5 weeks.
6. Restricted birds were hypoglycaemic from weeks 2 to 7 and hyper‐lipacidaemic throughout. A negative correlation between plasma glucose and free fatty acids was found. 相似文献
2. The parameters that were measured were growth performance indices, serum biochemical activity as well as ash and mineral (i.e. Ca, P, Mg, Fe, Cu and Zn) content of tibia, breast muscle and liver.
3. Results indicated that both supplemental B and dietary Ca and aP had marginal effects on performance indices of chickens grown for 42 d.
4. There were positive correlations (linear effect) between B concentrations of serum, bone, breast muscle and liver and the amount of B consumed.
5. Serum T3 and T4 activities increased linearly with higher B supplementation.
6. Increasing supplemental B had significant implications on breast muscle and liver mineral composition. Lowering dietary Ca and aP level increased Cu content in liver and both Fe and Zn retention in breast muscle. Tibia ash content and mineral composition did not respond to dietary modifications with either Ca–aP or B.
7. The results also suggested that dietary contents of Ca and aP do not affect the response to B regarding tissue mineral profile. Dietary combination with B and PHY did not create a synergism with regard to growth performance and bioavailability of the minerals. 相似文献
2. Diets prepared by serial dilution of a concentrated protein mixture, well‐balanced with respect to all essential amino acids, were fed in three experiments, while in a fourth experiment, a lysine‐deficient protein mixture was used.
3. Response curves relating body‐weight gain to increasing concentrations of protein and of lysine intake are presented.
4. A table is presented from which optimum protein intakes can be calculated according to changes in input and output costs and changes in growth potential of the chickens. 相似文献
2. A total of 72 chickens from each of these breeds were randomly distributed to 4 pens to provide equal numbers of two replicate pens maintained as infected and control (uninfected). All chickens in infected groups were inoculated orally with 1 × 108 CFU (1 ml dose) of a field isolate of S. gallinarum, at the age of 8 and 16 weeks given over 5 consecutive days. Growth performance, clinical signs, gross pathological lesions and antibody responses were measured.
3. A significantly higher mortality was observed in the brown layers compared with the white layers, and clinical signs and mortality were absent in village chickens. However, a large number of birds with gross lesions and high antibody titres were detected in village chickens, indicating that birds had the disease subclinically. Commercial breeds had a significantly higher body weight, feed intake and feed conversion efficiency.
4. There was a significantly lower proportion of positive reactors in village chickens in the whole-blood agglutination test (35%) compared to brown (100%) and white (90%) layers even after the second inoculation. Uninfected birds were negative in all groups. The indirect enzyme-linked immunosorbent assay confirmed these observations.
5. These results suggest that the indigenous breed had superior natural resistance to S. gallinarum than the commercial breeds. 相似文献
2. The experimental groups (1.25%, 1.5% and 2.5% DSS) showed clinical signs, such as loose stools and weight loss, which increased with additional treatment days and, as expected, the effects of DSS-induced intestinal inflammation were time and dose-dependent.
3. After 10 d, histological manifestations were evident, including goblet cell depletion, mucus layer loss, significantly shorter villi and a thinner total ileal mucosa.
4. The d(-)-lactate value, which was used as a gut leakiness indicator, was significantly increased in the 2.5% DSS group.
5. Expression of the inflammatory cytokines interleukin-1Beta, tumour necrosis factor alpha and interleukin-10 in the serum significantly increased with DSS treatment.
6. This study indicates that the experimental intestinal inflammation induced by DSS is an ideal model to study the pathogenic mechanisms of intestinal inflammation in chickens and to test the efficacy of therapies. 相似文献
2. Numbers of selected groups of faecal bacteria were not reduced by erythrosine.
3. No effect could be detected in the organoleptic properties of chickens processed after treatment with erythrosine. 相似文献
2. A total of 176 one-day-old male broiler chicks (Ross 308) were assigned to four treatments including control treatment (basal diet), 0.1% MCFA, 0.2% MCFA, and 0.3% MCFA treatments according to a completely randomised design. Each treatment consisted of 4 replicates with 11 chicks for each replicate. In MCFA treatments, the amounts of MCFAs were added to basal diet as a substitution of soybean oil.
3. There were not any significant differences in body weight, feed intake, and feed conversion ratio among the treatments in different stages of the experiment. However, MCFA treatments had a lower abdominal fat and a higher breast yield compared to control treatment.
4. Blood glucose, cholesterol, and low-density lipoprotein (LDL)-cholesterol concentrations were significantly decreased, but blood high-density lipoprotein (HDL)-cholesterol concentration was significantly increased in the MCFA treatments compared to the control treatment.
5. These results indicate that dietary MCFA has a positive effect on the health and productivity of broiler chickens. 相似文献
2. In both groups the water intake and water loss at all temperatures were well balanced.
3. Water to food ratios and water content of excreta of normal hens increased with increasing temperature.
4. The wetter excreta at 35 °C are caused by an increased urine production and not by an increased water passage through the alimentary tract. 相似文献
2. Plasma concentrations of glucose were reduced by fasting with reductions of 24.9% (19-d-old), 22.6% (33-d-old) and 17.9% (47-d-old) in broiler chickens fasted for 12 h.
3. Plasma concentrations of insulin decreased with fasting. For instance, circulating concentrations of insulin declined after 6 h of fasting by 45.7%, 54.7% and 50.0%, respectively, in 19-d-old, 33-d-old and 47-d-old broiler chickens.
4. Plasma concentrations of glucagon were increased by fasting. Plasma concentrations of glucagon were elevated by 3.79% (19-d-old), 3.51% (33-d-old) and 3.79% (47-d-old) with 6 h of fasting and remained elevated with 12 h, 18 h and 24 h of fasting. 相似文献
2. Results showed a significant difference on the anticoccidial efficacy 6 d after treating infected animals with Baycox (Et: 99.69% and Ea: 99.52%) or gen-TTZ (Et:85.71% and Ea 81.81%).
3. Gen-TTZ-treated animals were less strongly carotenoid-pigmented than Baycox-treated broilers. Mean plasma carotenoid concentrations were significantly higher in groups treated with Baycox than in broilers given gen-TTZ.
4. Treatment of animals with Baycox led to a significant decrease in ability of the peripheral blood mononuclear cells to produce ROS in contrast to gen-TTZ-treated groups. Baycox, but not generic toltrazuril, increased IL-10 and decreased tumour necrosis factor alpha (TNF-α) concentrations in chickens infected with E. tenella and E. acervulina.
5. It is suggested that differences in anticoccidial efficacy may be observed when using a generic toltrazuril product. Hence, in addition to plasma profiles of drugs, standardised clinical control tests may be necessary for generic formulations, particularly if other parameters are important to achieve a better control of coccidiosis. 相似文献
2. In the UL, IMF in breast muscle (4.65%) was 1.38 times that in the DL (3.36%). The IMF content in leg muscle was also higher in UL compared to DL.
3. There were significant differences in growth, carcase traits and meat quality between the two selected lines. However, ultimate pH (pHu) was lower and lightness (L*) and yellowness (b*) were higher in breast muscles of UL birds compared to DL birds. Similar trends for pHu and L* value between UL and DL were observed in leg muscle.
4. Significant mRNA expression differences of the heart fatty acid-binding protein (H-FABP) gene between UL and DL were observed in breast muscle, and a negative correlation between expression of mRNA and IMF% in breast and leg muscles pointed to H-FABP as a potential selection candidate gene. 相似文献
2. IBV variants were isolated from broiler flocks with enteric and respiratory problems in two regions of Brazil. The USP-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into IBV-antibody-free broilers and specific pathogen-free (SPF) chickens to determine tissue tropism and pathogenicity and compared with an IBV variant (USP-50) isolated from chickens showing signs of respiratory disease only.
3. Both USP-10 and USP-50 strains caused similar pathological patterns by either route of inoculation. Both variants were detected in respiratory and non-respiratory tissues, including the kidney, intestine and testis.
4. Broilers were more susceptible to infection than SPF chickens, and seroconversion was detected in all of the chicks. 相似文献
2. Preparations from leg weakness cases displayed neither accelerated muscle fatigue nor decreased nerve conduction velocities as compared with control preparations.
3. The muscles of the TA‐treated turkeys but not of those with naturally occurring leg weakness were hyperexcitable relative to those of controls.
4. Post‐tetanic potentiation of preparations from both naturally occurring and experimentally‐induced cases of leg weakness was less than that of control preparations, both before and after partial neuromuscular blockade, significantly so at the lower pulse frequencies used.
5. It is tentatively suggested that the last finding might be of significance in explaining the clinical signs of leg weakness and might be associated with a disordered calcium metabolism. 相似文献
2. Some plants and their respective volatile oils and extracts have the potential to alleviate coccidiosis and reduce its severity.
3. Most plant bioactives improve some, but not all, aspects of coccidiosis with variable effectiveness against different species of Eimeria.
4. Difficulties in comparing research findings have arisen from the use of different experimental models, different active components and infectious dose of Eimeria.
5. Current knowledge of their potential anti-coccidial effects may provide guidance for the use of botanical extracts in the control of the coccidiosis. 相似文献
2. The tendency was for the proteolytic activity to be increased and the amylase activity to be decreased in conventional chicks. These differences were statistically significant in the caecum.
3. All of the additional proteolytic activity detectable in the caecum of conventional chicks was inhibited by soyabean trypsin inhibitor. However, the increased activity was not detectable in the small intestine and was, therefore, not due to pancreatic trypsin.
4. Attempts to demonstrate in vitro bacterial production of a trypsin‐like enzyme were unsuccessful. 相似文献