添加黏土及植物残体混合物对沙质土壤中呼吸作用和微生物量碳、氮有效性的影响

Crop yields in sandy soils can be increased by addition of clay-rich soil, but little is known about the effect of clay addition on nutrient availability after addition of plant residues with different C/N ratios. A loamy sandy soil(7% clay) was amended with a clay-rich subsoil(73% clay) at low to high rates to achieve soil mixtures of 12%, 22%, and 30% clay, as compared to a control(sandy soil alone) with no clay addition. The sandy-clay soil mixtures were amended with finely ground plant residues at 10 g kg~(-1): mature wheat(Triticum aestivum L.) straw with a C/N ratio of 68, mature faba bean(Vicia faba L.) straw with a C/N ratio of 39, or their mixtures with different proportions(0%–100%, weight percentage) of each straw. Soil respiration was measured over days 0–45 and microbial biomass C(MBC), available N, and p H on days 0, 15, 30, and 45. Cumulative respiration was not clearly related to the C/N ratio of the residues or their mixtures, but C use efficiency(cumulative respiration per unit of MBC on day 15) was greater with faba bean than with wheat and the differences among the residue mixtures were smaller at the highest clay addition rate. The MBC concentration was lowest in sole wheat and higher in residue mixtures with 50% of wheat and faba bean in the mixture or more faba bean. Soil N availability and soil p H were lower for the soil mixtures of 22% and 30% clay compared to the sandy soil alone. It could be concluded that soil cumulative respiration and MBC concentration were mainly influenced by residue addition, whereas available N and p H were influenced by clay addition to the sandy soil studied.     

长期施肥对土壤中硒的累积及其生物有效性的影响

Continuous applications of organic and inorganic fertilizers can affect soil and food quality with respect to selenium(Se) concentrations.A long-term(over 20 years) experimental field study,started in 1989,was conducted to investigate the changes in soil Se fractions and its uptake by crops,as affected by different fertilizer practices,in the North China Plain with an annual crop rotation of winter wheat and summer maize.The long-term experiment was arranged in a complete randomized block design consisting of 4replications with 7 fertilizer treatments:1) organic compost(OC),2) half organic compost plus half N-P-K chemical fertilizers(OC+ NPK),3) N-P-K fertilizers(NPK),4) N-P fertilizers(NP),5) P-K fertilizers(PK),6) N-K fertilizers(NK),and 7) an un-amended control.Soil samples from the surface(20 cm) were collected in 1989,1994,1999,2004 and 2009 to characterize Se and other soil properties.In 2009,the average soil Se concentrations in the treatments(149 ± 8 μg kg~(-1)) were higher than those in the soil samples collected in 1989 at the beginning of the experiment(112 ± 4 μg kg~(-1)),and decreased in the order of OC OC + NPK NPK≈NP PK ≈NK control.Sequential extraction showed the oxidizable fraction(50.06%± 3.94%) was the dominant form of Se in the soil,followed by the residual fraction(24.12%± 2.89%),exchangeable fraction(15.09%± 4.34%) and Fe-Mn oxides fraction(10.73%± 4.04%).With an increase of soil K,the exchangeable Se concentrations in the soil increased.The Se concentrations in the soil tillage layer(0-20 cm) were mainly related to soil organic carbon(SOC),although different contributions came from atmospheric deposition,irrigation and fertilizers.With the accumulation of SOC,the uptakes of soil Se by two crops were inhibited.For the OC and OC + NPK treatments,Se concentrations in wheat grains were lower than the critical standard of Se in stable food(100 μg kg~(-1)).Additionally,Se concentrations in grains were also decreased by the deficiencies of major soil nutrients,especially P.     

长期施肥对潮土耕层土壤和作物籽粒微量元素动态的影响

Micronutrient status in soils can be affected by long-term fertilization and intensive cropping.A 19-year experiment (1990-2008) was carried out to investigate the influence of different fertilization regimes on micronutrients in an Aquic Inceptisol and maize and wheat grains in Zhengzhou,China.The results showed that soil total Cu and Zn markedly declined after 19 years with application of N fertilizer alone.Soil total Fe and Mn were significantly increased mainly due to atmospheric deposition.Applications...     

长期施肥对酸性土壤磷形态及有效性的影响

为明确长期施肥对集约化果园土壤磷形态、分布特征及其有效性的影响，本研究对琯溪蜜柚主产区29个果园的土壤进行调查研究，采用张守敬和Jackson的酸性土壤无机磷分级方法，研究蜜柚果园土壤磷素累积对土壤磷形态及有效性的影响。结果表明：在集约化蜜柚果园中，土壤磷素累积丰富，随树龄增加土壤速效磷含量上升显著，且土壤磷形态在不同土层存在显著性差异；当全磷含量≤0.5 g/kg时，土壤磷形态主要以有机磷、铁磷为主，随着全磷含量的上升，铝磷含量、占比均显著上升；多元线性回归和逐步回归分析结果表明本试验中铝磷与速效磷相关性最好。果园土壤已经形成一个巨大的磷库，且有效性较高，可适当减少磷肥投入，降低土壤磷含量，减少经济成本，提高生态效益。     

长期施肥及覆膜栽培对土壤锌、铜、锰的形态及有效性影响的研究

通过在沈阳棕壤上的5年定位试验,对不同施肥处理土壤进行锌、铜、锰各形态系统分析,结果表明：覆膜栽培加剧了土壤中锌、铜、锰各形态的消耗,不同施肥处理,三种元素各形态含量存在较大差异,土壤有机态锌、铜,氧化物结合态铜,交换态锰,碳酸盐结合态锰是玉米吸收的主要形态。     

长期定位施肥对土壤铁、锰形态及剖面分布的影响

本文采用改进的BCR连续提取法,对沈阳农业大学棕壤肥料长期定位试验地31年不同施肥处理土壤铁和锰含量变化及其剖面变异规律进行研究。结果表明:与试验前相比,耕层土壤两种元素水溶态和弱酸溶态含量都有所增加,而可还原态和残渣态含量则有不同程度的减少;铁和锰都以残渣态和可还原态为主。在空间分布上,两种元素弱酸溶态和可氧化态含量随土层的加深而减少,残渣态则相反。研究表明,有机肥能在一定程度上改变铁和锰在各形态间的分配,并且有机肥能活化残渣态铁和锰。     

武夷山茶园土壤铜、铅和锰形态及茶叶有效性特征

通过测试武夷山19个茶园土壤及茶叶中的Cu、Pb和Mn元素全量及土壤元素化学形态组成,分析土壤三种元素的生物可利用性和茶叶对其富集能力,并研究土壤理化性质对茶叶元素含量的影响。结果表明,武夷茶区土壤和茶叶Cu、Pb和Mn全量属于安全范围内。元素形态中,Cu以残渣态为优势;Pb、Mn以残渣态和铁锰氧化态为主,三者的生物可利用性大小排序为:Pb>Mn>Cu。富集系数比较得出,茶叶对土壤Cu、Mn富集能力较强,Pb的富集系数较低。相关性结果表明,该茶区土壤酸化很可能已经导致了土壤锰在一定量上的淋溶与流失;土壤有机质的增加,促进茶叶对Cu、Mn的吸收、累积,并降低土壤铅的有效性;重元素全量不能充分反映土壤对茶叶的有效性,赋存形态对合理评估土壤对茶叶的有效性更加重要。     

气象因子和土壤质地影响下刺槐蒸腾对土壤水分有效性的响应

On the Loess Plateau of China, a dry soil layer may form due to excess transpiration, leading to degradation of black locust(Robinia pseudoacacia) stands. In order to better manage projects involving black locust, this study was intended to investigate the response of black locust transpiration rate to soil water availability as affected by meteorological factors using two representative soils(loamy clay and sandy loam) on the Loess Plateau. Four soil water contents were maintained for black locust seedlings grown in pots initially outdoors and then in a climate-controlled chamber, by either drying or irrigating the pots. In both environments, daily transpiration rates were related by a power function to air temperature and by a logistic function to reference evapotranspiration(ET0). Transpiration rates were more susceptible to changes in the meteorological conditions in the sandy loam than in the loamy clay soil. The transpiration rate in the well-watered treatment was greater for black locust grown in the sandy loam than in the loamy clay soil. Normalized transpiration rates were unaffected by ET0 until a critical value of soil water content(θc) was attained; the θc value decreased significantly for the loamy clay soil but increased significantly for the sandy loam soil when ET0 increased. These suggested that the effect of the meteorological condition on the transpiration characteristics of black locust was dependent on soil texture.     

植被覆盖和雨强对附着在沉积物上的养分流失, 泥沙颗粒组成及体积分形维数的影响

Vegetation and rainfall are two important factors affecting soil erosion and thus resulting in nutrient loss in the Chinese Loess Plateau.A field experiment was conducted to investigate the effects of rainfall intensities(60,100 and 140 mm h-1) and vegetation(Caragana korshinskii) coverages(0%,30% and 80%) on soil loss,nutrient loss,and the composition and volume fractal dimension of eroded sediment particles under simulated rainfall conditions.The results showed that vegetation cover,rainfall intensity and their interaction all had significant effects on sediment transport and the sedimentbound nutrient loss.Higher rainfall intensity and lower coverage led to higher sediment and nutrient losses.Positive linear relationships were observed between soil loss and nutrient loss.The treatments showed more significant effects on the enrichment ratio(ER) of nitrogen(ERN) than organic matter(EROM) and phosphorus(ERP).Compared with the original surface soil,the eroded sediment contained more fine particles.Under the same coverage,the clay content significantly decreased with increasing rainfall intensity.The ER of sediment-bound nutrients was positively correlated with that of clay,suggesting that the clay fraction was preferentially eroded and soil nutrients were mainly adsorbed onto or contained within this fraction.There were increments in the fractal dimension of the sediment particles compared to that of the original surface soil.Moreover,the fractal dimension was positively correlated with clay,silt,and sediment-bound OM,N,and P contents,whereas it was negatively correlated with sand content.This study demonstrated that fractal dimension analysis can be used to characterize differences in particle-size distribution and nutrient loss associated with soil erosion.     

黄土沟壑区小流域不同地形条件下土壤锰的形态分布及其有效性

黄土高原复杂的地形条件因改变了土壤的基本性质而成为影响土壤锰素有效性的主要原因,本文研究了黄土沟壑区不同地形条件对锰的形态分布及其有效性的影响。结果表明,研究区土壤有效锰的含量因地形条件不同差异很大,有效锰在土壤剖面呈下降趋势,在塬面土壤含量最高,沟道土壤含量最低。交换态锰在塬面、梯田土壤含量较低,在坡地和沟道土壤含量较高;塬面、梯田和坡地土壤碳酸盐结合态和有机结合态锰的含量高于沟道土壤,氧化物结合态锰的含量显著低于沟道土壤;矿物态锰以塬面土壤最低,沟道和梯田土壤较高,坡地土壤居中。土壤交换态锰是有效锰的直接来源,但因其含量低而对锰的有效性贡献很小;有机结合态锰是有效锰的潜在来源,对锰的有效性贡献最大。     

Carbon availability and microbial biomass in soil under an irrigated wheat-maize cropping system receiving different fertilizer treatments

Seasonal changes in carbon availability and microbial biomass were studied in soil under an irrigated wheat-maize cropping system receiving different fertilizter treatments over the past 10 years. Treatments included N-100 and N-200 (urea at 100 and 200kgNha^–1 year^–1, respectively), FYM-16 and FYM-32 (farmyard manure at 16 and 32tha^–1 year^–1, respectively) and a control (unfertilized). Aerobically mineralizable carbon (AMC; C mineralized after 10 days aerobic incubation at 30°C) increased (13–16%) under wheat at both rates of urea whereas under maize it increased (22%) only with the lower rate of urea. Farmyard manure also increased the content of soil AMC under both crops, the effect being two- to threefold higher under wheat than under maize. Urea application caused an 32–78% increase in the specific respiratory activity (SRA) under wheat but caused an 11–50% decrease during the maize season. Farmyard manure also resulted in a higher SRA under both crops but only at the higher application rate. Under wheat, microbial biomass C (MBC) decreased in urea-treated plots but showed a slight increase at the higher rate of FYM. During the maize season, MBC was higher under both urea (42–46%) and FYM (36–47%) treatments as compared to the control. Microbial biomass turnover rate was highest for FYM-32 (2.08), followed by FYM-16 and urea treatments (1.35–1.49); control plots showed a turnover rate of 0.82. The higher AMC and SRA during the active growth period of wheat than that of maize indicated that root-derived C from wheat was higher in amount and more easily degradable. Received: 16 April 1996     

Manganese toxicity in bush bean as affected by concentration of manganese and iron in the nutrient solution

An experiment was conducted to clarify the relationship between Mn toxicity and Fe deficiency in bush snap bean (Phaseolus vulgaris L. cv. ‘Wonder Crop No. 2'). Seedlings were grown in full strength Hoagland No. 2 solution at pH 6.0 for ten days. Six concentrations of Mn as MnCl₂.4H₂O were used in combination with three concentrations of Fe as FeEDTA.

Toxicity symptoms, induced by low levels of Mn (0.1 ppm and above), included: small brown necrotic spots and veinal necrosis on primary leaves; necrosis on primary leaf petioles; interveinal chlorosis, with or without brown necrotic spots, on trifoliate leaves; and brown necrotic spots on stipules. Manganese toxicity symptoms were alleviated or prevented by increasing Fe concentration in the nutrient solution.

Manganese concentration in the leaves increased with increasing Mn and decreased with increasing Fe concentration in the nutrient solution, Iron concentration in the roots increased with increasing Fe concentration in the nutrient solution; however, Fe concentration in the leaves was not significantly affected by increasing Mn concentration in the solution culture. Manganese toxicity symptoms developed when Mn concentration in the leaves reached about 120 ppm.

A decrease in the Fe/Mn ratio in the nutrient solution resulted in a proportionate decrease in that of the leaves. Manganese toxicity symptoms occurred when the Fe/Mn ratio in the solution was 10.0 and below, or when the ratio in the leaves was less than 1.5. The ratio of Fe/Mn in the solution required for optimum growth of ‘Wonder Crop No. 2’ bean, without Mn toxicity symptoms, was in the range of 20.0 to 25.0.

Results indicate that the chlorosis on bush bean leaves induced by excessive Mn in the nutrient solution was due to excessive accumulation of Mn and not to Fe deficiency.     

Long-term fertilization and manuring effects on physically-separated soil organic matter pools under a wheat-wheat-maize cropping system in an arid region of China

With increasing food demand worldwide, agriculture in semiarid and arid regions becomes increasingly important, though knowledge about organic matter (OM) conserving management systems is scarce. This study aimed at examining organic C (OC) and nitrogen (N) concentrations in various soil OM pools affected by 26-years application of chemical fertilizer and farmyard manure at an arid site of Gansu Province, China. Macro OM (>0.05 mm) was extracted by wet sieving and then separated into light macro OM (<1.8 g cm⁻³) and heavy macro OM (>1.8 g cm⁻³) sub-fractions; bulk soil was differentiated into free particulate OM (FPOM, <1.6 g cm⁻³), occluded particulate OM (OPOM, 1.6-1.8 g cm⁻³) and mineral-associated OM (>1.8 g cm⁻³). OC and N concentrations of heavy macro OM and FPOM were slightly affected by long-term N fertilization alone and its combination with P and K, but their magnitudes of change had not significantly contributed to total soil OC and N concentrations. Farmyard manure increased light macro OC and N by 58 and 70%, heavy macro OC and N by 86 and 117%, free particulate OC and N by 29 and 55%, occluded particulate OC and N by 29 and 55%, and mineral-associated OC and N by 44 and 48%, respectively, compared to nil-manure. Mineral fertilization improved soil OM quality by decreasing C/N ratio in the light macro OM and FPOM fractions where farmyard manure was absent. Organic manure led to a decline of the C/N ratio in all physically-separated OM fractions possibly due to the increased input of processed organic materials. We found about two thirds of macro OM was actually located within 2-0.05 mm organo-mineral associations or/and aggregates. In conclusion, this study stresses the vital importance to apply organic manure to the wheat-corn production system characterized by straw removal and conventional tillage in the region.     

An assessment of various pools of organic phosphorus distributed in soil aggregates as affected by long-term P fertilization regimes

The phosphorus (P) forms in long-term fertilization determine the fate and transport of P in soil. However, the fate of various pools of organic P of added P in the long-term measured with sequential chemical fractionation is not well-understood. Four soil physical aggregates (>250, 125–250, 63–125 and <63 μm) from 0- to 20-cm depth after 35 years of long-term fertilization treatments including control (CK), nitrogen and phosphorus fertilizer (NP) and NP combined with farmyard manure (NPM) under continuous winter wheat were separated using settling tube apparatus. Results showed that the application of long-term P fertilization had no apparent effects on promoting the mass proportion of soil aggregates except for >250 μm, where the NP and NPM treatments significantly increased the mass proportion by 60% and 70% over CK, respectively. Compared with CK, P fertilizer (NP and NPM) treatments significantly increased organic P (Po) contents in each size aggregate. In particular, mean labile Po increased by 35% and 246%, moderately labile Po by 125% and 161%, nonlabile Po by 105% and 170% and total Po (TPo) by 101% and 178%, respectively, under NP and NPM treatments, respectively. There was a significant correlation between soil organic carbon (SOC) and Po fractions. SOC was exponentially positively correlated with labile Po but linearly positively correlated with moderately labile Po, nonlabile Po and TPo fractions among soil aggregates. A reduced C:Po ratio (<100) in soil aggregates among treatment indicates a large amount of available P accumulated in soils, and soil P loss risk in the study site is still high. Our results show that the Po pool measured by sequential chemical fractionation may represent an important, yet often overlooked, source of P in agriculture ecosystems. According to the result, long-term mineral P fertilizer combined with organic amendments better sustains soil structural stability in large aggregates, contributing more Po availability in the moderately labile P followed by labile P in soil aggregates.     

Status of paddy soils as affected by paddy rice and upland soybean rotation in northeast Japan,with special reference to nitrogen fertility

To investigate the current available nitrogen (N) and chemical properties of paddy soils affected by crop rotation between irrigated paddy rice (Oryza sativa L.) and upland soybean [Glycine max (L.) Merr.] (paddy-upland rotation), topsoils were collected from 22 fields of four different farmers in the northeastern region of Japan. Regardless of organic material application, a significant negative correlation was found between available soil N and an increase in the proportions of upland seasons to total crop seasons after the initiation of paddy-upland rotation. Soil total N and total carbon (C) also tended to decrease with an increase in upland frequency. In fields with repeated applications of cattle manure compost, the soil available N was higher than in fields where only crop residue was applied. A significant negative correlation was also found between the soil available N:total N ratio and upland frequency. This indicates that the part of soil N related to available N was notably lost by the use of paddy fields as upland fields. In order to sustain available soil N over the minimum suitable level of 80?mg?kg^?1, upland frequency should not exceed 65% when only crop residues and no other organic materials are applied. The upland frequency can be raised by the repeated application of organic materials which maintain a higher level of available soil N. The results imply that care should be taken to maintain the N fertility of paddy soil at a suitable level in paddy-upland rotation, and that upland frequency and organic materials applied are important factors to do this.     

栽培模式及施肥对玉米和大豆根际土壤磷素有效性的影响

栽培模式及施肥管理对作物吸收利用土壤磷素的影响较大,本研究为探明玉米/大豆套作系统作物根系交互作用下根际土壤无机磷组分动态变化特征,利用盆栽试验测定了玉米/大豆套作(M/S)、玉米单作(MM)和大豆单作(SS)3种栽培模式以及不施肥(CK)、施氮钾肥(NK)和施氮磷钾肥(NPK)3种施肥处理下玉米和大豆地上部生物量及吸磷量和根际与非根际土壤速效磷、无机磷组分含量,以期为优化玉米/大豆套作系统磷素管理提供理论依据。研究结果表明同一施肥水平下,套作玉米的籽粒产量显著高于单作玉米;施磷显著提高了单作玉米籽粒产量,而对套作玉米籽粒产量影响不大。无论施肥与否,套作大豆秸秆及籽粒产量均高于单作大豆。所有施肥处理均表现为套作模式下单株作物地上部磷积累量显著高于单作模式。玉米成熟期,CK、NK处理下套作玉米根际土壤速效磷含量分别比单作玉米高54.2%和71.8%;大豆始花期,NPK处理下套作大豆根际土壤速效磷含量比单作大豆高19.8%。大豆成熟期,NK、NPK处理下套作大豆根际土壤速效磷含量分别比单作大豆高23.8%和108.0%。无论是单作还是套作模式,玉米根际土壤Al-P含量在3个施肥处理下均低于非根际土壤。CK和NK处理下单作玉米根际土壤Al-P含量分别是套作玉米的1.19倍和1.22倍;NPK处理下单作玉米根际土壤Fe-P含量是套作玉米的1.21倍。在CK、NK和NPK施肥处理下,单作大豆根际土Al-P含量分别是套作大豆1.12倍、1.30倍和1.25倍,单作大豆非根际土Al-P含量分别是套作大豆的1.22倍、1.30倍和1.06倍。CK、NK处理下单作大豆根际土壤Fe-P含量分别是套作大豆的1.47倍和1.12倍。研究得出结论,低磷条件下,与单作相比,玉米/大豆套作更有利于作物对土壤Al-P、Fe-P的活化吸收。     

Influence of organic amendments on Fe,Cu, Mn,and Zn availability and clay minerals of different soils

The effects of application of composted olive mill wastewater sludge (A) and depotassified sugarbeet vinasse (V) on total diethylenetriaminepentaacetic acid (DTPA) and sequential extracted micronutrients were investigated. The mineralogy of the fine fraction of soils was also studied. The soils used were a Typic Rhodoxeralf (soil R), a Typic Xeropsamment (soil S), and a Typic Xerorthent (soil C). Fertilization with A and V during 3 years, in general did not significantly affect the total concentration of Fe, Cu, Mn, and Zn versus the control. However, the elements extracted with DTPA generally increased with the organic amendments, more with A than with V. The BCR (European Community Bureau of Reference) sequential extraction indicated that the addition of organic matter generally increased Zn and Mn in the two more available fractions. A great amount of Fe was found in the second and third fractions from the unamended and amended soils. Nevertheless, the sum of the three fractions was enhanced for the organic amendment, except for calcareous soil. The distribution of these elements in the different fractions was significantly affected by the type of soil. The addition of both fertilizers caused modifications in particle size and consequence redistribution of the calcite content between the different fractions.     

集约水稻系统下施肥对土壤有机氮形态及供氮的影响

Changes of soil organic nitrogen forms and soil nitrogen supply under continuous rice cropping system were investigated in a long-term fertilization experiment in Jinhua, Zhejiang Province, China. The fertilizer treatments included combination of P-K, N-K, N-P, and N-P-K as well as the control. After six years of continuous double-rice cropping, total soil N and hydrolysable N contents remained stable in plots with N treatments, while the hydrolysable N contents were substantially reduced in those plots without N application. Compared to the unbalanced fertilization treatments, P and K increased the percentage of hydrolysable ammonium N in the total soil N with the balanced application of N, and also maintained higher rice grain yields and nitrogen uptake. Grain yield was positively correlated with total N uptake （r = 0.875^＊＊）, hydrolysable N （r = 0.608^＊＊）, hydrolysable ammonium N （r = 0.560^＊＊） and the hydrolysable unknown N （r = 0.417^＊＊）. Total N uptake was positively correlated with hydrolysable N （r = 0.608^＊＊）, hydrolysable ammonium N （r = 0.440^＊＊） and hydro, lysable unknown N （r = 0.431^＊＊）. Soil nutrient depletion and/or unbalanced fertilization to rice crop reduced N content in soil microbial biomass, and therefore increased C/N ratio, suggesting a negative effect on the total microbial biomass in the soil.