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棉花不同生育期SOD同工酶与品种抗黄萎病性相关性的研究 总被引:8,自引:0,他引:8
凝胶电泳测定不同棉花品种子叶期、4叶期、蕾期、铃期、吐絮期等5个不同生育期的叶片内SOD同工酶,结果其谱带数目相应为4、8、8、4、3,随着棉花的生育进程,SOD同工酶活性由弱一强一弱。在棉花生长前期子叶期、4叶期,抗、感黄萎病品种之间,接菌与不接菌棉株间,SOD同工酶谱活性差异不明显;在蕾期,虽然品种间的SOD同工酶谱活性差异不明显,但接菌的棉株E1、E5、E6三条酶带活性强于不接菌棉株,E3活性则相反;在铃期,抗、耐品种较感病品种E2谱带活性强,接菌棉株较不接菌棉株增加一条E3b谱带。感病品种泗棉2号上显症棉株叶片体内SOD同工酶较无病症叶增加一条E4酶带;不同病级的叶片内,SOD同工酶的活性差异不大。至吐絮期,不同品种棉株及显症叶与无病叶间的SOD酶活性又趋相同。 相似文献
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用聚丙烯酰胺凝胶电泳法分析了355份甘薯品种的过氧化物酶同工酶,明确了甘薯的过氧化物酶同工酶酶谱表现,结合品种表现分析,初步建立甘薯品种资源的过氧化物酶同工酶酶谱档案。 相似文献
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马铃薯过氧化物酶同工酶分析 总被引:1,自引:0,他引:1
同工酶在作物分类鉴别、亲缘关系以及遗传分析等方面广泛应用,在水稻、玉米等有性繁殖材料上报导很多,但在无性繁殖材料马铃薯上尚未见报导。本文通过对30个马铃薯品种的过氧化物同工酶分析,以期初步查明马铃薯过氧化物同工酶的表现形式,及其品种之间的酶谱差异,探索鉴别品种的方法,为马铃薯品种资源的保存和品种质量管理提供依据。1材料与方法1.1试验材料:由呼盟马铃薯脱毒中心提供试管苗30份.其中包括国内材料9份(1-9),丹麦材料6份(10-15),荷兰6份(16-21),美国材料9份(22-30);上述材料中部分品种用花期后的叶片。… 相似文献
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冬小麦不同发育时期过氧化物酶同工酶研究 总被引:3,自引:0,他引:3
利用聚丙烯酰胺凝胶垂直板电泳技术对 18个冬小麦品种 4个发育期的POD同工酶进行了研究 ,发现在不同发育时期冬小麦根和叶POD同工酶酶谱均有不同程度的变化。返青期根POD同工酶谱变化较明显 ,在A区增加 1条RA1酶带 ,在C区增加 1条RC1酶带 ,而其他发育期无这 2条酶带或只有痕迹带 ;拔节期叶POD同工酶谱C区酶带比其他发育期多 1条LC1酶带 ;同时发现根RC4 酶带、叶LC5酶带在 4个发育期均较为稳定 ,表现为一级带 ;A区的RA2 酶带也较为稳定 ,在不同发育期均表现为二级带 ;冬小麦 18个品种间POD同工酶谱无明显差异 相似文献
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转几丁质酶和葡聚糖酶双价基因棉花株系对黄萎病的抗性 总被引:4,自引:1,他引:3
采用人工病圃、重病田及苗期室内鉴定相结合的方法,研究了转双价(Chi+Glu)基因的棉花株系61217-1对棉花黄萎病的抗性。结果表明,转基因棉花株系61217-1在人工病圃连续3年达抗病水平,病指极显著低于其受体中棉所24。2009年该转基因株系在河南安阳、江苏大丰及新疆石河子棉花黄萎病重病田的病指分别为18.7、18.1和16.7,均极显著低于受体中棉所24的病指。该转基因株系对来自河北、湖北、新疆的3个强致病力落叶型菌株均达抗病水平,病指分别为16.4、16.8和15.6,也极显著低于受体中棉所24的病指。表明转双价(Chi+Glu)基因的棉花株系61217-1具有优异稳定的抗黄萎病性。 相似文献
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棉花黄萎病菌致病类型研究 总被引:3,自引:0,他引:3
以研究手段为主线,从形态学特征、凝胶电泳、血清学反应、营养体亲和性及几项分子生物学技术等方面讨论了目前国内外在划分棉花黄萎病菌种内致病类型上的研究进展状况,并在本文基础上作了总结。 相似文献
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Li Shezeng Niu Luxin Li Bochao Chen Xiuye Liu Chang Lu Xiuyun Guo Qinggang Ma Ping Ma Zhiying 《棉花学报》2021,32(6):501-521
[Objective] The aim of this study is to investigate the cotton differential metabolites in the interaction between Verticillium dahliae and cotton plant, and to explore new clues for the further study on the defense mechanism of cotton against V. dahliae. [Method] To obtain the cotton root, stem and leaf samples of the pathogen treated and healthy controls, the upland cotton cultivar CCRI 24 was selected as tested cotton material in this paper, and inoculated with V. dahliae conidia or sterile water by injuring root at the 2-true-leaf stage. The 70% (volume fraction) methanol extracts of these samples were separated and detected by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS), and the metabolite data were obtained by the online XCMS software. Through multivariate statistics and student’s t-test, the differential metabolites were investigated. The types of metabolites were putatively identified based on the comparison of the experimental molecular mass and the monoisotopic accurate molecular mass of cotton metabolites. [Result] The efficiency of negative ion mode was higher than that of positive ion detection mode in the cotton metabolites in UPLC-ESI-MS analysis. 576 ions mainly distributed in cotton root were found in the cotton seedling tissues including root, stem and leaf, which were the distinguished differential metabolites between V. dahliae treatment and healthy control. Among them, 77 ions were identified as sesquiterpenoids, diterpenoids, flavonoids, carbohydrates, aliphatics and phenols. In addition to sesquiterpenes, 17 compounds, including caffeic acid, astragalin, isoastragalin, dillenetin, ent-catechin, gossypetin 8-rhamnoside, gossypicyanin, herbacetin 7-glucoside, leucocyanidin, quercetin 3'-glucoside, quercetin 3-glucoside, quercetin 7-glycosides, α,2',3,3',4,4',6-heptahydroxychalcone 2'-glucoside, melibiose, sucrose, sucrose 6-phosphate and 1-tetratriacontanol, had not been reported in the literatures on the interaction between V. dahliae and cotton, which may be the novel pathogenesis-related metabolites on cotton Verticillium wilt. [Conclusion] The putative pathogenesis-related metabolites of cotton Verticillium wilt may play an important role in the interaction of cotton defense against V. dahliae, which provides an important clue for exploring the new resistant mechanism of cotton to Verticillium wilt. 相似文献
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北方植棉区棉花黄萎病菌生理分化类型研究 总被引:17,自引:5,他引:17
通过对采自北方植棉区落叶和非落叶病株的51个棉花黄萎病菌系致病力分化测定结果,确定我国北方植棉区存在落叶类强致病力菌系;混合类中度致病力菌系及非落叶类弱致病力菌系的3个致病类型,并分别占测试菌的41.51%、35.85%和22.64%,其中9个强致病菌系致害棉苗出现落叶程度与对照菌的落叶程度相当或略重,表明河北、山东、河南植棉区重病田有落叶型黄萎菌系。病菌培养性状研究,得出3个培养型,即黑色菌落型、灰白色菌落型与白色菌落型。明确了黄萎病菌生长最佳温度为25℃,这一结果同田间黄萎病大发生要求的温度一致。 相似文献
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河北省棉花黄萎病菌致病性的研究 总被引:20,自引:9,他引:20
在棉花苗期采用塑料营养钵底部定量注菌液法接种,于昼温25~26℃,夜温20~22℃的人工培养室中测定了自河北省重点植棉县分离的40个棉花黄萎病菌单孢菌系和外地5个菌系的致病性。以中棉石系亚、海岛棉海7124、陆地棉93抗12、辽棉5号、冀棉11号、农大3656个品种为鉴别寄主,以病情指数作为划分抗、感类型的标准,根据在鉴别寄主上的反应,45个供试菌系可被划分为致病力强的Ⅰ型、致病力中等的Ⅱ型和致病力弱的Ⅲ型,分别占53.5%、28.9%、17.8%。Ⅰ型菌系除使中棉表现抗(耐)病外,海岛棉和陆地棉均表现感病。该类菌系遍布冀中南棉区,其中有7个菌系可使部分品种表现落叶,证明河北省已有落叶型菌系存在。试验还表明,海7124、93抗12、冀棉11号和农大3654个棉花品种对黄萎病鉴别力较强,可作为鉴别寄主加以应用 相似文献
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棉花黄萎病菌与品种互作的格局分析 总被引:1,自引:1,他引:1
以23个棉花品种(系)接种18个黄萎病菌株的抗感表现(病情指数)为分析对象,在聚类分析的基础上用双标图方法研究了寄主 病原的互作格局。结果表明:23个棉花品种分为6个抗病性不同的品种组,18个菌株聚为5个致病力不同的菌株群。5031和50312组成的品种组G1对所有菌株群具有最高抗性,可作为抗黄育种的高抗质源。来自河北成安的VD25与来自江苏常熟的XS4、XS6和XS7组成的菌株群S4与G5品种组存在互作,其致病力最强,是棉花黄萎病的优势菌株群,在抗黄育种和抗黄鉴定中应予以高度重视。S2和S3菌株群与56%的参试品种构成的3个品种组间存在互作,是潜在的优势菌株群。具平均抗病性的G2品种组是黄萎病菌的优良寄主。 相似文献
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陆地棉黄萎病抗性遗传分析 总被引:6,自引:6,他引:6
以抗落叶型黄萎病棉花品系常抗棉,耐黄萎病品系中5173、河北抗黄、山东抗黄及感病品种TM 1、军棉1号、新陆早1号、感病1号8个材料进行8×8半双列杂交,对亲本及F1的黄萎病株率及病情指数等主要性状进行了研究,采用MINQUE(1)法估算方差分量,采用AUP法预测遗传效应值。遗传估算方差结果表明,在病圃人工接菌条件下,品种平均病指及收获期剖秆病指均以加性遗传效应为主。遗传力分析表明,黄萎病的广义和狭义遗传率均达极显著。基因效应预测值表明,抗及耐黄萎病品种与感病品种之间存在极显著差异。协方差分析表明,表示黄萎病抗性两个性状(成株期平均病指及收获期剖秆病指)之间存在极显著的正相关,而黄萎病与产量性状之间存在极显著负相关。 相似文献
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[Objective] The purpose of this study is to analyze the resistance mechanism of island cotton under Verticillium wilt stress and to find possible resistance genes. [Method] The cotton resistance mechanism under the stress of Verticillium dahliae was studied by protein two-dimensional electrophoresis and mass spectrometry at the protein level. [Result] In the leaves, eleven proteins were down-regulated and 15 proteins were up-regulated after 2 hours of inoculation with Verticillium dahliae. The down-regulated proteins were mainly related to photosynthesis and carbon assimilation, and then we deduce that Verticillium dahliae is mainly broken cotton photosynthetic system to cause cotton susceptibility. The up-regulated proteins were mainly related to photosynthesis and benzoquinone reductase, beta-D-galactosidase, 14-3-3f protein and other disease-resistant protein. [Conclusion] It is speculated that the defense mechanism of island cotton on Verticillium wilt may occur at two levels: one is passive defense, it is manifested that the isoproteins of the down-regulated proteins such as chloroplast II AB binding protein and ribulose diphosphate carboxylase are highly expressed to maintain the stability of the island cotton photosynthetic system, the histone and 14-3-3f protein are highly expressed to keep the cell stability; the second is active defense, it is manifested that the high expressed β-D-galactosidase and benzoquinone reductase may be involved in the resistance of island cotton to Verticillium wilt. 相似文献
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陆地棉品种对多个大丽轮枝菌菌株的抗性分析 总被引:1,自引:1,他引:1
对几个陆地棉黄萎病抗源用多个菌株分别接种鉴定。结果表明,它们对不同菌株的抗性可能是由不同基因决定的。例如R01中有多个抗不同大丽轮枝菌菌株的抗病基因;R18能抗4个落叶型菌株,且表现一致,这说明R18的抗病基因与R01的抗病基因在本质上可能不同。R01和R03中的2个株行能抗或耐所有试验用菌株尤其是落叶型菌系。R18的选择过程为选育棉花高抗黄萎病品种提供了可能参考的实例。抗源材料的分析结果表明,一个品种或株行的抗性鉴定必须进行两次以上,在感病株行中分离出来的抗病单株,是新品种、新材料的希望所在,也是不能遗传的假抗病材料(诱导性抗病)的最大怀疑对象。 相似文献