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《饲料与畜牧》2010,(9)
龙眼是著名的水果和重要的药材,其壳中含黄酮类等活性物质,具有重要的作用。以龙眼壳为原料,用95%的乙醇作溶剂,采用微波辅助法提取其中的黄酮类等活性物质,先进行单因素实验,研究处理时间(30s,60s,90s,120s,150s)、料液比(1:4,1:6,1:8,1:10,1:12)、微波功率(160w,320W,480W,640W,800W)对提取率的影响;然后进行3因素3水平正交试验,选择处理时间(30s,60s,90s)、料液比(1:4,1:6,1:8)、微波功率(160W,320W,480W)。试验结果表明:在室温25℃条件下,最佳提取条件为料液比1:4,微波处理时间30s,微波处理功率为160W。 相似文献
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龙眼是著名的水果和重要的药材,其壳中含黄酮类等活性物质,具有重要的作用。实验以龙眼壳为原料,用95%的乙醇作溶剂,采用微波辅助法提取其中的黄酮类等活性物质,先进行单因素实验,研究了处理时间(30s,60s,90s,120s,150s)、料液比(1∶4,1∶6,1∶8,1∶10,1∶12)、微波功率(160W,320W,480W,640W,800W)对提取率的影响;然后进行3因素3水平正交试验,选择处理时间(30s,60s,90s)、料液比(1∶4,1∶6,1∶8)、微波功率(160W,320W,480W)。试验结果表明:在室温25℃条件下,最佳提取条件为料液比1:4,微波处理时间30s,微波处理功率为160W。 相似文献
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为优化微波辅助提取山竹壳多糖的工艺条件,并研究其抗氧化活性,本试验考察了微波功率、微波时间、液料比3个因素对山竹壳多糖提取量的影响,在单因素试验的基础上,采用Box-Behnken响应面试验法对山竹壳多糖的微波辅助提取工艺条件进行考察,并通过测定山竹壳多糖对DPPH自由基和羟基自由基(·OH)的清除能力来评价其抗氧化性。结果表明:山竹壳多糖的最佳提取工艺为微波功率550 W、微波时间190 s、液料比35:1(mL/g),在此条件下,山竹壳多糖的提取量为17.83 mg/g(n=3,RSD=0.31%)。山竹壳多糖浓度为1.4 mg/mL时,对DPPH自由基和羟基自由基(·OH)清除率分别为88.31%和86.01%,IC50值分别为0.65 mg/mL和0.79 mg/mL。经Box-Behnken响应面优化得到的微波辅助提取工艺稳定、可靠,可用于山竹壳多糖的提取。山竹壳多糖具有较好的抗氧化活性。
[关键词] 山竹|植物多糖|响应面法|抗氧化 相似文献
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绿色饲料添加剂是当前研究的方向,本实验以红皮洋葱为原料,研究其抗氧化作用。用95%乙醇作溶剂,通过微波辅助法提取洋葱精油,料液比为1:5,微波功率160W,微波时间160S,微波处理之后浸泡2h,浸泡温度55℃,抽滤后减压旋转蒸发除去溶剂得洋葱精油。然后将其加入猪油和花生油中,添加浓度分别为0、0.07%、0.1%、0.2%和对照组0.05%的BHT(二叔丁基羟基甲苯)。采用烘箱贮藏法,以过氧化值(POV)和酸价(AV)为指标研究其对猪油和花生油的抗氧化作用。结果表明:洋葱提取物对猪油和花生油有一定的抗氧化作用,且具有剂量关系。 相似文献
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《饲料广角》2017,(6)
采用微波辅助提取米糠中原花青素,并对提取液的稳定性进行研究。试验研究了乙醇体积分数、料液比、微波时间、微波温度、微波功率等因素对米糠中原花青素提取的影响,并以这五个单因素为影响因子,原花青素得率为指标,通过正交优化了微波提取米糠原花青素的工艺,得最佳工艺条件为:料液比为1:20g/m L,微波温度60℃,微波时间90s,微波功率300W,乙醇体积分数60%时,此条件下原花青素得率达2.729%。米糠中原花青素提取液的稳定性试验结果表明,在弱酸至中性条件下比较稳定,避光有利于原花青素提取液的稳定保存,除了弱碱性的饲料添加剂碳酸氢钠和谷氨酸钠外,常用的饲料添加剂对原花青素提取液稳定性影响不大。 相似文献
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《饲料工业》2017,(18):38-43
试验以枸杞鲜花为试材,采用微波法提取其中多酚并进行体外抗氧化作用的研究以溶剂乙醇浓度、提取时间、微波功率作为枸杞鲜花多酚提取试验的主要影响因素,依据回归分析法确定出多酚提取得率以作为响应值的单因素响应面和等高线,分析因素的显著性和交互作用进而优化提取工艺,并将提取得到的枸杞鲜花多酚进行体外化学抗氧化性试验。结果表明,枸杞鲜花多酚的最佳提取工艺:乙醇浓度78.6%,提取时间149 s,微波功率63.2 W,多酚得率为0.057 4%;枸杞鲜花多酚还原力FRAP值为497.9/μl;对DPPH的IC50值为1.4701mg/l;对ABTS~+的IC_(50)值为0.178 6 mg/l;对NO_2~-的IC_(50)值为0.413 3 mg/l。 相似文献
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Evidence for the non-involvement of the uterus in the lifespan of the corpus luteum in the cyclic dog 总被引:1,自引:0,他引:1
Progesterone levels in peripheral blood of dogs were analysed during the cycle in which hysterectomy (n = 5) or sham surgery (n = 3) was performed as well as during the cycle of dogs (n = 5) hysterectomized at least one year prior to this study; the data were compared with the findings in control dogs (n = 3). The averages of the duration of the luteal period observed in the three experimental groups were not significantly different from those of control dogs. Immediately after surgery, the progesterone level decreased from 25 to 50% of the presurgical level, but returned to presurgical level in about four days. Prolactin levels were elevated for about 30 h after surgery. Nevertheless, the averages of the mean prolactin levels for each animal during the luteal period of the experimental groups were not significantly different from those of control dogs. It is concluded that in the dog, the uterus is not involved in the lifespan of the cyclic corpus luteum. 相似文献
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嗅源是军犬嗅觉作业训练的依据.军犬鉴别中嗅源能力的培养在整个鉴别训练中占有重要地位,关系到鉴别训练的成败和鉴别能力的提高.所以,军犬在嗅认嗅源时必须按照训犬员的指令充分、细致地感受嗅源气味,并且对嗅源气味产生兴奋反应.如何培养军犬充分嗅认嗅源以及及时解决嗅认过程中出现的问题,成为我们进行鉴别训练的关键.…… 相似文献
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Meschan EM Peham C Schobesberger H Licka TF 《Veterinary journal (London, England : 1997)》2007,173(3):578-584
As there is no statistical evidence that saddle fit influences the load exerted on a horse's back, this study was performed to assess the hypothesis that the width of the tree significantly alters the pressure distribution on the back beneath the saddle. Nineteen sound horses were ridden at walk and trot on a treadmill with three saddles differing only in tree width. Kinetic data were recorded by a sensor mat. A minimum of 14 motion cycles were used in each trial. The saddles were classified into four groups depending on fit. For each horse, the saddle with the lowest overall force (LOF) was determined. Saddles were classified as "too-narrow" if they were one size (2 cm) narrower than the LOF saddle, and "too-wide" if they were one size (2 cm) wider than the LOF saddle. Saddles two sizes wider than LOF saddles were classified as "very-wide". In the group of narrow saddles, the pressure in the caudal third (walk 0.63 N/cm(2)+/-0.10; trot 1.08 N/cm(2)+/-0.26) was significantly higher compared to the LOF saddles (walk 0.50 N/cm(2)+/-0.09; trot 0.86 N/cm(2)+/-0.28). In the middle transversal third, the pressure of the wide saddles (walk 0.73 N/cm(2)+/-0.06; trot 1.52 N/cm(2)+/-0.19) and very-wide saddles (walk 0.77 N/cm(2)+/-0.06; trot 1.57 N/cm(2)+/-0.19) was significantly higher compared to LOF saddles (walk 0.65 N/cm(2)+/-0.10/ 0.63 N/cm(2)+/-0.11; trot 1.33 N/cm(2)+/-0.22/1.27 N/cm(2)+/-0.20). This study demonstrates that the load under poorly fitting saddles is distributed over a smaller area than under properly fitting saddles, leading to potentially harmful pressures peaks. 相似文献
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Observations on the Pathology of the Spleen of the Cat 总被引:1,自引:0,他引:1
Abstract— Lesions were found in the spleen of four of sixty-three cats examined post mortem in the department, and comprised two examples of lymphosarcoma, one of chronic fibrinous peritonitis and one of subcapsular haematoma. Abnormalities were found in two cat spleens received from veterinary surgeons in general practice. In one cat the abnormalities were an haemangiosarcoma with secondary deposits in the liver and in the other extensive mast cell infiltration. In addition lesions were detected in the spleen of three of eighty-seven cats examined post mortem in general practice. The lesions were: multiple secondary deposits of adenocarcinoma from a primary tumour of the mammary gland, a ruptured spleen and a foreign body below the splenic capsule. Résumé— L'examen microscopique de soixante-trois chats pratiqué dans le service a permis de rencontrer quatre fois des lésions de la rate, dont deux lymphosarcomes, une péritonite capsulaire fibrineuse et un hématome sous-capsulaire. D'autre part des lésions ont été trouvées dans deux rates de chat expédiées par des vétérinaires exerçant en pratiaue générale. Chez un chat il s'agissait d'un htmangiosarcome avec métastases hépatiques et chex l'autre d'une infilt ation diffuse par des mastocyta. De plus, on a rencontré des llésions dnas la rate de trois chats parmi quatre-vingt sept autopsies effectuées dans le cadre d'une clientèle génárale. Ces iésions étaient dés métastases multiples d'un adénocarcinome dont la tumeur primitive siègeait dam la glande mammaire, une rupture de la rate et un corps érangers sou capsulaire. Zusammenfassung— Läsionen der Milz wurden bei vier von dreiuiidsechzig in dieser hbteilung sezierten Katzen gefunden, und zwar in zwei Fällen ein Lymphosarkom, in einem Falle eine chron-ische fibrinöse Peritonitis und in einem ein subkapsuläres Hématom. Anomalien fanden sich auch in der Milz von zwei Katzen, die aus der tierxrztlichen Praxis eingesandt wurden. Bei der einen Katze war dies ein Hämangiosarkom mit Metastasen in der Leber, bei der anderen eine weitläufige Infiltration der Mastzellen. Außerdem wurden bei drei von siebenundachtzig in der Praxis sezierten Katzen Milzläsionen gefunden. Diese bestanden am multiplen Metastasen eines Adenokarzinoms der Brustdrüse, einer Milzzerreissung und einem Fremdkörper unterhalb der Milzkapsel. 相似文献