昆明犬成熟精子超微结构的研究

应用透射电镜技术,对3头昆明犬精子的超微结构进行了观察。结果表明,昆明犬精子头部呈卵圆形,长约5.05μm,宽约0.91μm。颈部和尾部横切近圆形。颈部很短,长约0.92μm,直径为0.63μm。尾部中段长约15.02μm,直径约0.86μm;轴丝为9 2型。中段与主段套管式连接。终段轴丝为9×2 2形式。通过观察,没有发现昆明犬精子的独征。同时,冷冻对一些精子细胞膜和顶体造成损害,表现为细胞肿胀,头部和尾部主段、终段质膜变薄、皱褶、破损或丢失;顶体肿胀,顶体外膜囊泡化或不连续,个别出现顶体全脱;中段线粒体较完整,冷冻对昆明犬精子的损害并不十分严重。     

西藏赘犬精子的超微结构观察

应用透射电镜技术，对冷冻复温后西藏赘犬精了的超微结构进行了观察。结果表明，西藏赘犬精子头部呈卵圆形，长约４．６２μｍ，宽约３．１３μｍ，厚约０．６６μｍ颈部和尾部横切近圆形，颈部很短，长约０．８８μｍ，直径为０．６３μｍ，尾部中段长约７．９μｍ，直径约０．８３μｍ轴丝为９＋２型；线粒体螺旋４２－４９转，中段与主段套管式连接，终段轴丝为９×２＋２形式，同时，冷冻对一些精子细胞膜和顶体造成损害，表现为     

马鹿冻精解冻后精子超微结构观察

实验利用透射电镜技术和扫描电镜技术对冷冻/解冻后的塔里木马鹿精子的超微结构进行了观察。结果表明:电镜下观察,塔里木马鹿精子头部呈扁平的卵圆形,长约5.95μm。颈部很短且不明显,长约0.45μm,宽为0.62μm。尾部中段横切面近圆形,直径约为0.58μm,轴丝为9×2+2型;线粒体鞘螺旋段为64～70转。尾部末段仅见质膜包围,9束微管和1对中央微管,形成9+2微管结构。冷冻/解冻后塔里木马鹿部分精子结构发生了变化,一些精子肿胀、质膜皱褶、扭曲,部分质膜损坏或溃散;顶体轻度肿胀,顶体外膜凸凹不平,形成突起。冷冻对头部破坏程度较小。     

德国牧羊犬精子超微结构及超低温冷冻对精子超微结构的影响

为了研究超低温冷冻前后德国牧羊犬精子超微结构的变化,采用扫描电镜和透射电镜对冷冻前后德国牧羊犬精子超微结构变化进行研究,结果显示在扫描电镜下,鲜精整体呈“蝌蚪状”,头部呈扁卵圆形.在透射电镜下精子纵切面头部呈“楔形”,头部细胞膜由外向内分别由质膜、顶体外膜、项体内膜和核膜组成.精子尾部中段横切面由外向内可见线粒体鞘膜、9束外周致密纤维,9对轴丝和2根中央微管.精子头长约为5.40 μm,头宽3.26 μm,颈长1.25 μm,颈宽0.55 μm,尾部中段长11.34 μm,中段直径0.87 μm,尾部长55.70 μm,线粒体螺旋数为44旋.鲜精和冷冻前精子头部、颈部和尾部形态变化差异均不显著(P＞0.05).超低温冷冻后精子头部、颈部和尾部发生形态变化的精子数极显著高于鲜精和冻前精子数(P＜0.01).冻后顶体发生明显形态变化的精子占54.21％,高于发生颈部形态变化(10.61％)和尾部形态变化(28.83％)的精子数.结果表明鲜精和冻前精子形态变化不显著,而超低温冷冻后精子头部、颈部和尾部发生形态变化的精子数显著高于鲜精和冻前精子数.     

马鹿精子形态与超微结构研究

文中对马鹿(Cervus elaphus)精子形态与超微结构进行了研究。马鹿精子经固定、脱水、置换、包埋和聚合,用超薄切片机切片,再用醋酸双氧铀、柠檬酸铅染片,最后于透射电镜下观察其超微结构的变化。观察结果表明:马鹿精子全长(58.75±2.35)μm,由头部(8.93±0.24)μm、颈部(1.00±0.16)μm和尾部(48.18±1.18)μm三部分组成;头部呈扁卵圆状,绝大部分被浓缩且电子密度较高的精核所占据,顶体似帽状扣在精核之上,约占头部的2/3,其前部较为膨大;颈部位于头部和尾部之间,这个区域较短;尾部可分为中段、主段和终段。马鹿尾部轴丝的结构类型为"9+2";微管结构类型为"9+9+2"。     

鸭精子超微结构初步探讨

在相差显微镜下,鸭精子的头部和尾部结构与鸡精子相似,鸭精子头部略长,尾部略短。在电子扫描镜下,可见到顶体异常精子呈现质膜膨胀。顶体外膜膨胀,顶体粒脱落,顶体脱落等。在电子显微镜下,鸭精子直径约5μ,顶体呈锥形,顶体柄伸入到核质内约1.1-1.2μ,颈部近中心粒形成的九组三联微管结构,外径约为0.23μ,远中心粒形成的九个卵圆球,每个球的横径约为0.15μ。螺旋线粒体部分全长约3μ(鸡为4.2-5μ),正常者表面平滑,异常者表面凹凸不平。尾部无中心纤丝区约1.55μ,该区后端2个卵圆形结构,是中心纤丝的起始部位。尾部主段起始于终环,长约54.3μ。尾部尾段长约0.9μ。     

梅花鹿精子冷冻前后形态和超微结构的研究

采用光学显微镜和电子显微镜对冷冻前后的梅花鹿精子的形态和超显微结构进行了观察。结果表明：梅花鹿精子全长６１．６±２．７０μｍ,其中头长７．１９±０．４７μｍ,中段长１２．０８±０．７５μｍ。线粒体的螺旋数是６３．６８±４．６６旋,中段线粒体每个螺旋约由３～５个线粒体组成。梅花鹿精子超微结构有３个特点：一是头部的厚度为牛、羊、猪精子的１／２;二是在中环处的质膜未见反折现象,并且主段与中段的联接是以套管式镶嵌;三是末段以９＋２结构变成２０根（１２＋７＋１）单丝管形式排列。冷冻处理可使梅花鹿精子顶体膨胀,顶体内容物丢失,顶体内发生空泡,顶体外膜自身囊泡化,线粒体发生断裂和丢失,末段纤维束因质膜丢失而分散成扫帚状。冷冻后的梅花鹿精子畸形率极显著增高（Ｐ＜０．０１）,冷冻解冻是致使梅花鹿精子顶体完整率和活力降低的主要原因。     

贵州小型猪精子显微结构研究

应用光学显微镜、透射电镜和扫描电镜对贵州小型猪精子的形态和结构进行了研究.结果表明,贵州小型猪精子由头、尾二部分组成.精子全长57.00±1.69 μm;头部呈椭圆形体,长为9.49±0.40 μm;尾部由颈、中、主和末段组成;尾部中央为9×2 2排列模式的轴丝,轴丝外围依次是外周致密纤维、线粒体螺旋鞘、纤维鞘、细胞膜;线粒体螺旋鞘仅存在于中段,外周致密纤维从主段到末段逐渐变细,并在主段近末端处分批终止,末段外围仅保留纤维鞘、细胞膜.贵州小型猪精子组织结构与人类接近,是研究人类男性生殖系统疾病较理想的动物模型.     

棕熊精子体外获能及异种穿卵的超微结构研究

对棕熊精子体外获能前后和异种穿卵的超微结构观察表明,棕熊精子全长77μm,由头、颈和尾3部分组成.头部长7.3μm,宽2.5μm,主要由核、顶体及顶体后区组成;颈部由中心粒和9条纵行分节的节往组成;尾部全长68.2μm,其中中段长13.2μm,线粒体为65～68旋,主段中央为“9 2”的微管结构,其外方被9条致密纤维和纤维鞘包裹.精子获能前群集成簇,运动缓慢;获能后精子呈超激活运动.获能的精子质膜膨胀,顶体外膜囊泡化,引起顶体反应,质膜并未参加囊泡化.顶体反应完成后,仅有顶体内膜包在精子核膜的外面.棕熊精子与仓鼠的卵相互作用,多以赤道段和顶体后区附着于卵膜.     

棕熊精子体外获能及异种穿卵的超微结构研究

对棕熊精子体外获能前后和异种穿卵的超微结构观察表明，棕熊精子全长７７μｍ，由头、颈和尾３部分组成，头部长７．３ｍ，宽２．５μｍ，主要由核、顶体及顶体后区组成；颈部由中心粒和９条纵行分节的节柱组成；尾部全长６８．２μｍ，其中中段长１３．２１ｍ，线粒体为６５－６８旋，主段中央为“９＋２”的微管结构，基外方被９条致密纤维和纤维鞘包裹。精子获能前群集成簇，运动缓慢；获能后精子呈超激活运动。获能的精子质膜膨     

Phase I evaluation of CCNU (lomustine) in tumor-bearing cats

1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.     

Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects

Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.     

Prevalence of gastric lesions in racing Alaskan sled dogs

Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.     

Internal Parasites and Haematological Values in Cattle Slaughtered in Buea Subdivision of Cameroon

Tropical Animal Health and Production -     

The Epidemiology of Gastrointestinal Nematodes of Dairy Cattle in Central Kenya

The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.     

Diversity of Ectoparasites in Sheep Flocks in Sa~o Paulo,Brazil

The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.     

Chemotaxis of macrophage in inflammation

Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.     

Evaluation of biohydrogenation rate of canola vs. soya bean seeds as unsaturated fatty acids sources for ruminants in situ

An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.     

Serum biochemical values and mineral element contents of tissues in yaks

Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein