Methodologic studies on protein metabolism and bioenergetics of protein deposition in growing animals. 4. Energy metabolism in chickens in connection with measurement of parameters of protein metabolism

In connection with the measuring of parameters of the protein metabolism in parallel experiments, the energy metabolism of 6 chickens (origin Tetra B) in the live weight range between approximately 100 and 1,800 g was determined under conditions of restricted energy supply. 3 animals each received a feed mixture containing 20% (animal group 1) and 38% (animal group 2) crude protein. The amount of feed was daily increased by 1.5 g DM. The digestibility of energy and nitrogen was independent of the age. 66.3 +/- 3.3% and 64.0 +/- 5.0% resp. of the metabolisable energy were utilised for protein and fat retention. The energy maintenance requirement, determined at a live weight of 2,000 g, was independent of protein supply and averaged in the two animal groups 434 +/- 40 kJ metabolisable energy/kg live weight 0.75 . d. The result of multiple regression was, for the growth period investigated, an energy maintenance requirement of 403 +/- 32 kJ metabolisable energy/kg live weight 0.75 . d. 1.77 and 1.38 J metabolisable energy resp. were required for 1 J protein or fat retention. The energy requirement for protein retention was independent of the degree of protein supply. The results from the measuring of energy metabolism are discussed in connection with the kinetic parameters of protein metabolism ascertained in parallel experiments.     

Involvement of food intake in the decreased energy retention associated with single deficiencies of lysine and sulphur-containing amino acids in growing chicks.

1. Growth and energy utilisation were determined in growing chicks fed ad libitum on diets deficient either in lysine (5.95 g/kg) or in sulphur-containing amino acids (SAA, 3.5 g/kg). Food intake, body weight gain, energy retained as protein and as fat, and total energy retention were significantly (P less than 0.05) reduced by single deficiencies of either lysine or SAA. 2. Another two experiments were conducted to determine if the decreased total energy retentions in chicks fed on diets deficient in lysine (experiment 3) or SAA (experiment 4) were associated with reduced food intake, by using tube-feeding to control the amount and pattern of food consumption. Chicks fed on diets deficient in lysine or SAA retained less energy as protein and more energy as fat than the control chicks. Neither total energy retention nor heat increment was affected by these deficiencies. Total energy retention was proportional to metabolisable energy (ME) intake alone. 3. It is concluded that the decreased total energy retentions caused by single deficiencies of lysine and SAA were associated with decreased food intake.     

Effect of iodine, copper and zinc supplements to rations with a high quota of rapeseed extract meal on the growth and thyroid function of fattening swine. 2. The effect of the iodine supplement on nutrient digestibility and on protein and energy retention

Three groups of six pigs each (live weight at the beginning of the experiment 20 kg) were given a cereal ration with 8% rape seed meal. Group I did not receive a J supplement and was fed ad libitum. The rations of groups II and III were supplemented with 1 mg J/kg feed. The feed intake of group II was limited to the amount consumed by group I (pair fed); group III received the feed ad libitum. The daily weight gains of the 3 groups were 327, 377 and 613 g, feed expenditure 3.79, 3.68 and 3.47 kg/kg gain. In an N balance experiment carried out with 3 animals each from groups I and II, 41.4% of the N taken in group I and 43.8% in group II were retained (p greater than 0.05). Liver, intestinal fat and leaf were heavier in group I (p less than 0.01), the protein content of the empty body, the bones and bristles significantly lower than in group II. Protein retention in group II was 14% higher than in group I. In case of iodine deficiency protein retention is lower, energy retention, however, is largely unchanged.     

The energy metabolism of growing swine in the body weight range of 10-50 kg. 2. Nitrogen and energy metabolism

Investigations were carried out about nitrogen and energy metabolism feeding rations with 17-24 and 44-47% crude protein content on maintenance and growing level to castrated male hybrid pigs of line 150. In growing periods the N deposition amounted to 10 g/animal.d (15 kg live weight), 18 g/animal.d (30 kg LW) and 21 g/animal.d (greater than 40 kg LW) on lower protein feeding level. In experiments with higher protein feeding level the corresponding results were 17, 22 and 22 g N deposition/animal.d. The partial utilization of metabolizable energy for deposition amounted to 70% for the rations with 17-24% protein content and to 59% for the rations with 44-47% protein content, without correlation to the animals development and the alternation in the protein feeding level. The results of regression analysis about maintenance requirement were 814, 775 and 806 kJ metabolizable energy/kg LW0.62.d in trials feeding rations with 17-24% crude protein content as well as 764, 846 and 818 kJ metabolizable energy/kg LW0,62.d in trials feeding rations with 44-47% crude protein content. 1,5-1,8 MJ metabolizable energy were used per MJ protein energy deposition and 1,3-1,4 MJ per MJ fat energy deposition respectively. The energy deposition per kg live weight gain amounted to values between 9 (10 kg LW) and 18 MJ (60 kg LW).     

Joint research on the precise determination of the energy and protein requirements of fattening pigs. 7. The effect of various energy levels on the performance and feed expenditure of fattening pigs

Scientific feeding experiments were carried out with 7 male castrated pigs of the genetic origin land race pig X large white with the test animals fed individually and the rations containing approximately the same amounts of protein, amino acids, minerals and vitamins but varied amounts of energy. Energy intake was adjusted to three levels: 100%, 85% and 70% of semi-ad-libitum intake (group 1 = 100%). With regard to protein supply there was a subdivision into 30-60 and 60-100 kg live weight. In addition to this, the digestibility of the feed mixture used and the N-balance were determined in both fattening periods. Having reached 100 kg live weight, the animals were slaughtered and a number of slaughtering parameters was determined. The average daily live weight gain in the three test groups was 749, 664 and 540 g over the whole fattening period. The feed expenditure was 3.7, 3.9 and 4.3 kg mixed feed per kg live weight gain so that, with regard to live weight performance and feed expenditure the group with the highest energy supply showed the best results. The level of energy supply had a remarkable influence on the composition of weight gain; thus the amount of fat in the carcass of the animals of groups 3 was by 1.8 kg and 2.5 kg resp. lower than of the animals in groups 2 and 1.     

Energy requirement for the maintenance and utilization of energy convertible to protein and fat deposits in piglets

In respiration experiments with 16 piglets the effect of feeding level on energy metabolism was studied with the aim of estimating energy requirement and costs of protein and fat deposition. Four groups of 4 animals each were fed on different levels of digestible protein and metabolisable energy (ME). Group 1 was fed intensively, whereas the piglets of group 2, 3 and 4 received 92, 76 and 55% respectively of the amounts given to group 1. In the group 1-4 mean daily weight gain was 457, 437, 360 and 205 g respectively. As a consequence the rearing period increased from 44 days to 46, 56 and 98 days. The variation in feed intake affected not only significant differences in energy deposition but also changes in gain composition. In the groups 1-4 the average energy deposition was 4.2 MJ, 4.0 MJ, 3.0 MJ and 1.4 MJ per day and protein gain exceeded fat gain in all groups. Estimations of energy requirement for maintenance were carried out by means of multiple regression analysis using different models. As a result a value of 428 MJ ME per kg live weight 0.75 was obtained and the models used have hardly shown any influence. It seems that higher values for maintenance requirement, as formerly published, are due to different conditions of livestock management, such as temperature. For all groups the average efficiency of ME-utilisation for growth was 0.77, ranging from 0.73 to 0.82. The variation can be attributed to the changes in protein and fat formation. The groups with a higher proportion of protein in the accretion utilised metabolisable energy more efficiently than the intensively fed group 1 with the highest proportion of fat, the difference between the groups being in the range from 0.73 to 0.82. The efficiency of ME-utilisation for protein deposition was calculated to be 0.83 and for fat deposition 0.73. As a higher coefficient for fat formation may be expected in the light of the high fat content in the ration, calculations with an assumed coefficient from 0.75 and 0.80 had been carried out, showing that the efficiency of ME-utilisation for protein gain would only decrease to 0.79 and 0.73 respectively. According to these results the statement must be called in question, that the energetic efficiency of protein deposition of about 50 to 55% - as measured in numerous experiments mainly with older pigs - can generally be accepted.     

Energy metabolism of growing broilers in relation to the environmental temperature

7 experiments with 6 chickens each (origin Tetra B) in the live weight range between greater than 100 and less than 300 g and up to 1800 g were carried out at environmental temperatures (ET) of 35, 30, 25 (2 experiments) 20 (2 experiments) and 15 degrees C. In the course of each experiment the chickens alternatively received feed mixtures containing 20 and 40% crude protein (3 animals/variant) for maintenance and weight gain (semi ad libitum). Energy metabolism was measured according to indirect calorimetry over a total of 645 metabolism periods. In the temperature range studied there was no compensation between thermoregulatory heat and heat from other metabolic processes. The partial utilization of metabolizable energy for energy retention in the body was independent of ET and remained in the limits between 71 and 73%. Energy utilization was dependent on the protein content of the feed. It decreased from 75 to 69% with the increase of the protein content from 20 to 40%. Energy requirement for protein retention varied between 1.67 and 1.89 kJ metabolizable energy/kJ and was independent of ET. Energy requirement (metabolizable energy) for the maintenance of the energy balance was independent of the protein content of the feed. It increased from 433 kJ/kg LW0.75.d at 35 degrees C to 693 kJ/kg LW0.75.d at 15 degrees C ET. The relationship between heat production and ET is parabolic. The thermoneutral temperature decreased from 35 to 25 degrees C in the course of development. In the live weight range of 300-500 g thermoregulatory heat production had its maximum with 19 kJ/kg LW0.75.d.K and decreased in the further development to 10-13 kJ/kg LW0.75.d.K.     

Interaction between the temperature of the surroundings, performance and level of feed for growing pigs

Investigations of the performance of growing pigs in the live weight range of 30 to 60 kg kept individually and in groups with ad libitum and rationed feeding were carried out in two air-conditioned rooms. The air temperature in the individual experiments was 20 degrees C, 10 degrees C and 5 degrees C, the relative atmospheric moisture in all experiments was between 70 and 80%. A diminished growth of 24 g per day per degrees C below 20 degrees could be ascertained for pigs kept individually. The diminishing of the growth can be compensated by an increase of the feed intake of 1.3 g per kg live weight, day and degrees C resp. the intake of metabolisable energy of 4 kcal per kg live weight, day and degrees C until the capacity of feed consumption is reached.     

Effect of protein restriction and refeeding on the nitrogen balance in piglets

In balance trials the effect of a 50% protein restriction with subsequent realimentation on N balance, endogenous N excretion, digestible and metabolisable energy was tested using 36 piglets. The dietary protein content during the restriction period from 5-12 kg live weight was 22.9 (control) and 12.4% of the dry matter and during the realimentation period from 12-18 kg live weight in both groups 17.2%. Protein quality and energy supply were not changed between the groups. Restricted protein supply proportionally reduced N retention with slightly improved efficiency of digestible N from 67 to 70%. During realimentation the previously reduced N supply did not influence N retention but increased the apparent N digestibility by 2% units. The endogenous N losses as well as digestible and metabolisable energy were not different between the groups. It was concluded that compensatory responses of the N metabolism might require a stronger dietary restriction and respectively or a longer restriction period.     

Joint research on the precise determination of the energy and protein requirements of fattening pigs. 4. The effect of the energy level on the fattening of Camborough-type hybrid swine

Scientific feeding experiments were carried out with 5 castrated male pigs and 4 female animals of the genetic origin "Camborough" in the live weight range between 30 and 100 kg with a subdivision at 60 kg. In each test group the animals were fed individually and received rations with approximately equal contents of protein, amino acids, minerals and vitamins but different energy contents. Energy intake was adjusted to 3 levels of 100%, 85% and 70% of semi-ad-libitum intake. Apart from that, the digestibility of the feed mixtures used in the 2 fattening periods was determined. Having reached a live weight of 100 kg, 7 representative animals per group were slaughtered and a number of slaughtering parameters were registered. In all three test groups the daily live weight gain over the complete fattening period was very high (846, 819 and 778 g). Though the weight gain in group 3 with 70% of the energy supply of group 1 is significantly lower than in the other two groups, it is, however, only 70 g lower than in group 1. Consequently, the feed expenditure (2.6 kg mixed feed per kg live weight gain) in group 3 is by 20% lower than in group 1, in which the feed expenditure is 3.2 kg mixed feed per kg live weight gain. There were no significant differences on the weight gain performances and the registered carcass parameters between sows and castrated male pigs. Observed deviating tendencies are discussed.(ABSTRACT TRUNCATED AT 250 WORDS)     

Energy requirements of young female cattle. 5. Energy intake and energy expenditure

From 6 experiments dealing with the development of young female cattle, partly from calf to calving, 660 metabolism periods were evaluated as to energy intake, live weight gain (LWG) and energy expenditure. Growth intensity was deliberately regulated through energy intake. In the experiment with the highest growth rate the average LWG was greater than 850 g/animal and day and in the experiment with the lowest growth rate at about 550 g/animal and day. Energy expenditure increased from 15 MJ net energy fatcattle/kg LWG at a live weight of 50 kg to 50-80 MJ NEFcattle/kg LWG in clear dependence on growth intensity. Comparative examinations of energy intake and energy requirement, estimated with equations forming the basis of recommendations for the energy supply of young female cattle in the framework of the GDR system of feed evaluation, lead to the conclusion that a new concept of identifying the energy requirement of young female cattle has to be developed.     

The energy metabolism of growing swine in the liveweight range of 10-50 kg. I. Study design, feed intake and digestibility

Studies of the energy metabolism at maintenance and growth levels after the feeding of rations with a crude protein content of 17-24% and 44-47% resp. were carried out with hybrid pigs of line 150 in the live weight range between 10 and approximately 50 kg. This paper gives information on the methods and the outlay of the experiment and presents results concerning feed intake, live weight development and digestibility. Feed intake increased on average with growing live weight by 30-35 g DM/kg live weight. Feed conversion ranged from 1.2 to 1.8 kg DM/kg live weight gain in the first period and from 2.3 to 3.2 kg DM/kg live weight gain in the last period. The digestibility of the energy in the rations with a crude protein content of between 17 and 24% averaged 80% and that of the rations with a crude protein content of 44-47% averaged 86%. In the course of ontogenetic development the digestibility increased up to about 30 kg LW. The influence of the nutritional level on the level of digestibility was unequal in the experiments. In one experiment a decrease (1% unit) and in two experiments an increase (1-3% units) of the digestibility after the feeding of growth level in contrast to maintenance level could be observed. The change of rations with a varying protein content did not result in an influence on the digestibility level in comparison with the constant feeding of one ration.     

Joint research on the precise determination of the energy and protein requirements of fattening pigs. 8. The effect of various protein levels on the performance and feed expenditure of fattening pigs

Live weight development and feed expenditure were investigated on 3 levels of protein supply and isocaloric rations in scientific feeding experiments with 10 castrated male pigs each of the genetic origin land race pig X large white fed individually in the live weight range between 30 and 100 kg with a subdivision at 60 kg. In the air-dried matter the crude protein content of the feed mixtures used in the first fattening period was 17.6; 15.7 and 13.7% resp. and in the second fattening period from 60 kg live weight onwards 14.9; 12.8 and 11.4% resp. for the 3 test groups. In both fattening periods the digestibility of the rations and the N-balance were determined at 3 representative animals from each of the 3 groups. The following average live weight gains were achieved in the 3 test groups: 1st fattening period: 662; 592 and 539 g resp.; 2nd X fattening period: 709; 676 and 644 g resp. Feed expenditure over the complete fattening period was 4.2; 4.5 and 5.2 kg air-dried matter per kg live weight gain. The highest protein supply resulted in the best live weight gain performance and the lowest expenditure.     

Lysine requirement of fattening boars in respect to their performance. 1. Influence of the consumption of energy and amino acids on the fattening performance

With a total number of 157 boars 9 feeding variants with different levels of energy and amino acids were checked. The weight increase per fattening day and the energy and lysine expenditure per kg live weight increase were determined. The insufficient supply with lysine had negative effects on the live weight increase and the energy expenditure during the whole fattening period. Between the variantes with a high supply with amino acids (120%) and the supply according to standard (100%) no significant differences concerning the live weight increase could be ascertained up to 90 kg live weight. Between 90 and 120 kg live weight the animals in the variant with medium energy level and 120% lysine supply achieved significantly higher increases than those in the standard variant. Up to 90 kg live weight the energy expenditure for all groups amounted to 2,08 kEFs (energetic feed units for pigs) per kg live weight increase. The most suitable results were achieved by the animals which received the variants rich in energy (1,760 resp. 1,770 kEFs per kg live weight increase). Above the 90 kg live weight limit the energy expenditure rose considerably, remained however under 2,000 kEFs per kg live weight increase for the boars of the variant with medium energy level and 120% lysine supply as well as for the variant rich in energy and 100% lysine supply. The lysine expenditure in the first variant amounted to 25.1 g per kg live weight increase and for the variant rich in energy to 20.6 g lysine per kEFs.     

套测法和直接强饲法测定肉雏鸭饲粮中小麦氨基酸及能量营养价值的比较研究

本试验旨在评价肉雏鸭饲粮中小麦氨基酸和能量的营养价值,并比较套测法和直接强饲法测定结果的差异。选取48只15日龄樱桃谷肉鸭(SM3品系),随机分为4个处理,每个处理12个重复,每个重复1只鸭,单笼饲养。处理1为基础饲粮组,处理2为80%基础饲粮+20%小麦组(套测组),处理3为100%小麦组(直接强饲组),处理4为饥饿组,用以测定内源损失。结果表明:1)小麦干物质中的粗蛋白质和总能含量分别为16.09%和18.56 MJ/kg。2)除了谷氨酸、脯氨酸、胱氨酸外,套测法测定的其余氨基酸的表观(真)氨基酸代谢率均高于直接强饲法测得到的结果(P<0.01);套测法和直接强饲法测得的总氨基酸的表观代谢率分别为86.59%和82.42%,真代谢率分别为92.36%和90.80%。3)2种方法测定的能量代谢率和代谢能差异不显著(P>0.05)。套测法测得小麦能量表观代谢率和真代谢率分别为77.51%和86.23%,表观代谢能和真代谢能分别为14.38和16.00 M J/kg;直接强饲法测得小麦能量表观代谢率和真代谢率分别为75.95%和84.81%,表观代谢能和真代谢能分别为14.09和15.74 M J/kg。由此得出,套测法和直接强饲法皆可用于评定小麦代谢能;而使用套算法测定小麦氨基酸代谢率时,合适的替代比例还有待进一步研究。     

Joint research on the precise determination of the energy and protein requirements of fattening pigs. 5. The effect of the crude protein concentration in the isocaloric diet on the fattening performance of swine of the Bulgarian Landrace

Live weight development and feed expenditure on 3 levels of crude protein supply and with isocaloric rations were investigated in scientific feeding experiments with 7 castrated male pigs each of the Bulgarian land race in the live weight range between 30 and 100 kg with a subdivision at 60 kg. The crude protein concentrations in the rations for the 3 groups were 17%, 15% and 13% in the first fattening period and 15% 13% and 11% in the second fattening period. In the two fattening periods the digestibility of the feed mixtures used was ascertained in experiments. The daily live weight gain in the three groups over the whole fattening period reached a high level of performance (836 g, 778 g and 757 g). The feed expenditure values were 3.3, 3.7 and 3.7 kg mixed feed (88% dry matter) per kg live weight gain. The highest protein supply resulted in the best weight gain performance and the lowest feed expenditure.     

Energy and nitrogen metabolism of chickens infected with either Eimeria acervulina or Eimeria tenella.

1. The effects of sublethal infections of E. acervulina and E. tenella on the energy and nitrogen metabolism of groups of five broilers aged 16 d were studied for 16 d in respiration chambers. 2. The metabolisable energy content of the diet for chickens infected with E. acervulina was 0.689 of its gross energy content and N retention was 42.5 g/100 g N intake compared with 0.738 and 47.1 g respectively, in uninfected pair-fed controls. Chickens infected with E. tenella were similarly affected. 3. Efficiency of utilisation of ME by chickens infected with E. acervulina was 0.43 during the first 8 d after infection, and 0.52 during the second 8 d compared with an overall efficiency by non-infected chickens fed ad libitum of 0.73. Maintenance energy requirement of infected chickens was higher during the first 8 d after infection than during the second 8-d period. 4. Body composition measurements showed that of the total gain in weight of chickens infected with E. acervulina, only 7.5 g/kg gain was fat and 213 g/kg was protein compared with 45 g and 210 g respectively for non infected chickens fed ad libitum. 5. E. acervulina and E. tenella infections reduced the apparent digestibility of total mineral, calcium and phosphorus.     

Growth studies in bulls of the black-and-white dairy cattle breed at a low feeding level. 1. Growth progress and fattening performance]

In 2 experiments with a total of 108 black-and-white dairy bulls feed intake, growth performance and energy expenditure for live weight and carcass production with low feeding intensity were ascertained. The performance level varied between 600 and 650 g; 24-month-old bulls achieved a final weight of 458 and 517 kg resp. Growth and retention performance were considerably influenced by the energy concentration of the ration and by daily energy intake.     

Influence of fibre on metabolisable energy of diet and performance of growing pullets in the Tropics

1. Diets in which maize and palm kernel meals were major ingredients and whose metabolisable energy values were varied by the inclusion of graded amounts of maize cob (50 to 200 g/kg diet) were fed to growing pullets for 9 to 21 weeks of age. 2. Apparent metabolisable energy decreased from 11.82 to 9.79 MJ/kg diet as neutral detergent fibre increased from 182 to 330 g/kg. 3. Metabolisable energy intake was significantly reduced when the maize cob content was above 100 g/kg diet. 4. Protein intake was unaffected by treatment but apparent nitrogen retention was significantly depressed for pullets receiving the diet with 200 g/kg maize cob. Birds on the diet did not come into lay during the study.     

Energy requirements of young female cattle. 6. Energy requirements

Results taken from 6 experiments with young female cattle comprising 477 metabolism periods served the derivation of the animals energy requirement in the development range greater than 125 kg live weight according to the factorial method. The energy requirement per kg LW0.75 and day, calculated from metabolism data, was independent of the stage of development and the intensity of feeding. It averaged 455 +/- 66 kJ metabolizable energy/kg LW0.75.d and 250 +/- 37 kJ NEFcattle/kg LW0.75.d respectively. The partial energy requirement for live weight gain, expressed in net energy fat, was equivalent to the energy content of the live weight gain. Energy retention and thus energy requirement per kg live weight gain increased with the live weight and reached a maximum of 26 MJ. Energy retention per kg live weight gain largely depended on the intensity of feeding and the stage of gravidity. Restrictive energy supply and progressing gravidity decreased energy content in the weight gain. The influences mentioned were taken into consideration on the derivation of the partial requirement for live weight gain. Equations were developed for the estimation of the energy requirement of young female cattle, which can be applied to both gravid and non-gravid cattle.