Interaction between hHBrk1 and WAVE1 protein

AIM: To identify the binding sites between hHBrk1 and WAVE1 protein.METHODS: PCR was applied to clone the WAVE1 gene and its truncated domains from human fetal brain library. The gene fragments were sub-cloned into eukaryotic expression vector. Co-immunoprecipitation assay was used to identify the binding sites between hHBrk1 and WAVE1.RESULTS: The sequences of WAVE1 and the truncated domains were successfully amplified and sub-cloned into pCMV-HA vector. An HA tag was added to the fused protein at N-terminal. The interaction between hHBrk1 and the Scar homology domain(SHD) of WAVE1 was identified by co-immunoprecipitation. We also observed that WAVE1 bound to the heptad repeat(HR) domain of hHBrk1, and the site-directed mutations at HR domain abolished the interaction between hHBrk1 and WAVE1.CONCLUSION: hHBrk1 may play an important role in migration of tumor cells through binding to SHD of WAVE1 protein.     

Progress in roles of MNX1 antisense RNA 1 in malignant tumor

MNX1 antisense RNA 1 （MNX1-AS1） is a newly discovered long non-coding RNA （lncRNA）, which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor.     

Expression of molecular markers Jab1 and Akt1 in recurrent nasopharyngeal carcinoma

AIM：To analyze the expression of Jab1 and Akt1 in paired human primary and recurrent nasopharyngeal carcinoma (NPC) tissues, to explore the relationships between their expression and molecular mechanism and further to identify the potential treatment target of rNPC. METHODS：Paired primary and recurrent NPC archival samples (n=45) from Affiliated Foshan Hospital and Affiliated Shantou Hospital of Sun Yat-sen University were collected. The expression of Jab1 and Akt1 was determined by the method of immunohistochemistry (SABC). The expression of Jab1 and Akt1 in CNE-1, CNE-2, CNE-2R and HONE1 cells before and after ionizing radiation (IR) was detected by Western blotting. RESULTS：The expression of Jab1 and Akt1 increased in recurrent NPC tissues compared with the primary tumors (both P<0.05). The expression of Jab1 and Akt1 increased in CNE-1, CNE-2R and HONE1 cells in comparison with CNE-2 cells. Furthermore, Jab1 expression was elevated in CNE-1, CNE-2, CNE-2R and HONE1 cells after receiving IR. CONCLUSION：Over-expression of Jab1 and Akt1 contributes to the resistance of NPC cells with irradiation, and induces recurrence of NPC. Therefore, Jab1 and Akt1 serve as potential tumor targets for the treatment of recurrent NPC.     

胡萝卜新品种天红1号的选育

天红 1号胡萝卜是以雄性不育系HLBcms0 0 1号胡萝卜为母本 ,以HLBm0 0 1号胡萝卜为父本杂交而成的全红型一代杂种。植株生长势强 ,株高 5 1.78cm ,叶丛直立 ,深绿 ,有 8～ 11片叶 ;肉质根整齐 ,圆柱形 ,根尖圆形 ,根长 17.2 4cm ,根粗 3.12cm ,根质量 12 2 .8g,根表皮、韧皮部和髓部均为桔红色 ,可溶性固形物含量为 10 .17%,胡萝卜素含量为 113.0mg·kg-1,质脆 ,味甜 ,口感好 ,适于鲜食和加工 ;生长期 10 0～ 10 5d(天 ) ,平均每 6 6 7m2 产量34 38kg。目前已在天津、山西、河南等地示范推广。     

薄皮甜瓜新品种泽甜1号的选育

泽甜1号是利用生态适应性强的自交系Tz98-2-2为母本,含糖量较高的薄厚中间型自交系Tz98-3-5-1为父本配制的一代杂种。早熟,幼瓜浅绿色,成熟瓜圆形、淡黄色,肉质甜脆、细腻、洁白,总糖含量10%左右。单瓜质量350~450g,每667m2产量1500~2000kg,抗霜霉病、白粉病,适宜东北、河北、山东等薄皮甜瓜产区栽培,种植面积已达2000hm~2。     

茄子新品种承茄1号的选育

承茄1号是以河北省地方品种巨佳1号经多代自交选育的优良株 系17#为母本，以引进的法国品种经多代自交选育的优良株系01#为父本杂交而成的茄子一代 杂种。该品种果实长卵圆形，果皮紫红色有光泽，单果质量391g。露地栽培平均 每667m2产量4936kg，高产者可达6000kg， 较抗黄萎病和绵疫病，适宜北方春露地及麦茬栽培。     

水果型黄瓜新品种浙秀1号的选育

浙秀1号是由雌性系XH65与XD23配制而成的水果型黄瓜一代杂种,植株生长势强,早熟性好;瓜条短圆筒形,瓜皮绿色有光泽,无刺;瓜长约15cm,横径2.4cm,平均单瓜质量83g,口感脆嫩;高抗白粉病、枯萎病,抗霜霉病,每667m2产量7000kg左右,适于全国各地温室、大棚冬春及夏秋季栽培。     

黄瓜新品种锦农绿1号的选育

锦农绿1号是以H40807为母本,以G51918为父本配制而成的一代杂种。植株长势强,主蔓结瓜为主,第1雌花着生于第2~3节,雌花节率高;瓜条直,长棍棒状,长35cm;瓜皮深绿色,有光泽,密生白刺;果肉浅绿色,质脆,味甜;抗黄瓜花叶病毒病,中抗细菌性角斑病;每667m2产量6000kg左右,适宜日光温室及春、秋大棚栽培。     

辣椒新品种浙椒1号的选育

浙椒1号是从杭州地方品种鸡爪椒中经连续多代选择而获得的优良高代自交系HP9801和国外引进的高代自交系HP9915配制的一代杂种。早熟,植株生长强健,分枝性强,连续结果性强;果实为短羊角形,纵径12~14 cm,横径1.0~1.2 cm,平均单果质量10 g;果面光滑,果形直,味微辣。较抗病毒病和疫病,每667 m2产量3 500 kg左右,适宜全国大部分地区早春保护地、春露地及秋延后栽培。     

早熟辣椒新品种濮椒1号的选育

濮椒1号是以湖南省地方品种中选出的优良自交系047-3-3为母本,以国外引进的黄金椒经多代自交纯化选育出的优良自交系黄-2-1为父本配制而成的辣椒一代杂种。早熟,始花节位为第8～10节,从定植到始收45d(天)左右;生长势强,连续坐果性好,株高64.18cm,开展度72.17cm;果实长羊角形,果长20cm,横径3.3cm,果肉厚0.28cm;商品果黄绿色,果面光滑顺直,味辣,商品性好;单果质量60g;田间对病毒病、疫病和炭疽病的抗性优于洛椒7号;春露地地膜覆盖栽培每667m2产量3170kg左右,适于保护地及早春露地栽培。     

Wound-induced production of 1-aminocyclopropane-1-carboxylic acid and accelerated senescence of Petunia corollas

Treatment of the corollas of Petunia multiflora cultivar ‘Gypsy’ with ACC (1mM) caused accelerated corolla wilting similar to that caused by stigma wounding, whereas AVG or AOA, inhibitors of ACC synthesis, delayed the response.Concentrations of endogenous ACC were found to increase significantly in the gynoecia and corollas of stigma-wounded flowers within 24 and 48 h, respectively; C₂H₄ production also increased in the gynoecia and to a lesser extent in the corollas.The results are discussed in relation to the possible hormonal change induced by stigma wounding or pollination.     

黄瓜新品种"吉杂迷你一号"的选育

"吉杂迷你一号"黄瓜是以欧引4-1-1-1-1为母本,以自交系S1-1-4-2为父本配制而成的华南型黄瓜一代杂种.植株生长势强,以主蔓结瓜为主,全株雌性,节成性强,结瓜数多,果实商品性状优良,短棒状,果长14～16 cm,单瓜重50～80 g,果腔小,果皮深绿色,有光泽,品质好,无刺无棱基本无果粉,果型整齐一致,品质好,肉质细脆,微甜有香气,1 hm2产量10万kg.适宜春、秋大棚和日光温室栽培.     

攀杂丝瓜1号新品种选育

攀杂丝瓜1号是以从重庆九龙坡丝瓜中选育的S8-6自交系为母本,以选自上海香丝瓜的自交系S4-3为父本,培育的早熟、丰产杂一代新品种,该品种植株生长势、分枝性强,坐果率高,果实长圆柱形,匀称,皮色深绿,表皮皱,瓜纵径26.5cm,横径5.5cm,单瓜质量285.8g,商品性好,味清甜,品质优,667m2产量2918.5kg。     

宛椒1号辣椒的选育

宛椒 1号辣椒是利用从“世界冠军”甜椒中选育的自交系 880 8和从邓州市农家品种羊角椒中选育的自交系 880 4配制而成的一代杂种。果实大牛角形 ,纵径 15 .5cm ,肩横径 3.4cm ,肉厚 0 .35cm ,单果质量 4 3g ,微辣带甜。早熟 ,从定植到始收约 4 4d(天 ) ,一般 6 6 7m2 产量 370 0～ 4 5 0 0kg。较抗疫病、炭疽病和病毒病。     

节瓜新品种夏冠1号的选育

夏冠1号是以自交系7213作母本,以自交系1632作父本配制而成的节瓜一代杂种。植株生长势较强,分枝性强,主侧蔓结瓜。早熟,春栽从播种至始收65~80d(天),夏秋栽从播种至始收45~55d(天)。瓜圆筒形,皮青绿色,有光泽,被茸毛,无棱沟,瓜长16~17cm,横径5.5cm,单瓜质量450~500g。肉质嫩滑,微甜。耐热、耐寒能力较强,高抗枯萎病,对疫病的抗性强于对照江心4号。每667m2产量3000~4000kg,适宜华南地区春、夏、秋季种植。     

辣椒新品种临椒1号的选育

临椒1号母本94-18是从尖椒22号中经提纯复壮、筛选出的优良自交系,父本94-33是国外引进品种匈奥804经多代自交分离出的优良自交系。该品种生长势强,果实羊角形,基部皱折,果色深绿,纵径23.0cm左右,横径3.0cm,单果质量40~60g,辣味浓。对疫病、病毒病和炭疽病的抗性强于对照尖椒22号。中熟,从定植到始收60d(天),一般每667m2产量2860kg左右,适宜鲜食、制酱。     

辣椒新品种齐杂尖椒1号的选育

齐杂尖椒1号是以98-4-48为母本,以98-5-33为父本配制的辣椒一代杂种。生长势强,株高70cm,开展度55cm;果实长牛角形,纵径25~28cm,横径4.4cm,肉厚0.26cm;果面光滑,辣味中等,肉质脆,VC含量1.15g.kg-1,可溶性固形物4.97%;坐果率高,整齐度好,每667m2产量4000kg左右。适宜黑龙江地区种植。     

早熟黄瓜新品种沂蒙1号的选育

母本YM-98-11来源于农家品种当地油皮黄瓜,父本YJ-99-6是笔者对1997年从日本引进的13个黄瓜品系进行筛选得到的优良株系。其杂种一代沂蒙1号,瓜长棒形,瓜把短,无棱无瘤,刺小且稀少,皮薄光滑油亮;瓜长20cm左右,横径4cm;肉质密,口感好,特别适合生食。抗枯萎病能力强,对霜霉病、白粉病的抗性和对照当地油皮相当。667m2产量在5000kg以上。适合鲁南地区春季保护地早熟栽培和春季露地栽培。     

秋甘蓝新品种秋甘1号的选育

秋甘1号是以胞质雄性不育系CMS021为母本,以97025-B3为父本配制的秋甘蓝一代杂种。中熟,从定植到收获74d(天)左右。株型开展,开展度74cm,外叶14片,灰绿色,叶面蜡粉多,叶缘有轻波纹,无缺刻。叶球绿色,紧实,扁球形,球高14.6cm,横径23.6cm,中心柱长6.2cm,单球质量约2.2kg,质地脆嫩,味甘甜,不易裂球,耐热,抗病毒病(TuMV),耐黑腐病。每667m2产量4600kg左右,适于全国各地秋季种植。