脂肪酸对基因表达的影响及调控

脂肪酸是一类重要的营养物质,是动物体内的能量来源,同时也是生物膜的组成成分,在细胞生化过程中也同样起着重要作用。研究发现,脂肪酸可通过对基因表达的影响,对代谢、生长发育以及细胞分化发挥重要的调控作用。真核生物基因表达的调控大致可分为转录前、转录、转录后、翻译和翻译后等5个阶段的调控。脂肪酸通过细胞膜受体信号途径和转录因子活化途径调节基因表达,而多不饱和脂肪酸主要从基因转录和mRNA的稳定性两个方面调节基因表达。文章综述了脂肪酸,尤其是多不饱和脂肪酸对基因表达的影响及调控机制。     

放牧时间对羊肉多不饱和脂肪酸沉积及氧化稳定性的影响

分析不同放牧时间对羊肉抗氧化性能的影响。选择40只乌珠穆沁去势公羔,按照体重和出生月份接近的同质原则随机分成5个处理组:舍饲组(0H,对照),放牧2 h组(2H)、放牧4 h组(4H)、放牧8 h组(8H)和放牧12 h组(12H)。舍饲组圈养,4个放牧组在清晨6:00出牧,依组次分别在8:00、10:00、14:00和18:00归牧。试验期102 d,预试期15 d,正式期87 d。放牧试验结束后,从各组挑选体重接近的6只屠宰,取背最长肌,测定多不饱和脂肪酸(PUFA)和抗氧化物浓度。结果表明,1)放牧时间显著影响羊肉中抗氧化物的浓度。舍饲组的丙二醛(MDA)含量显著(P<0.001)高于4个放牧组,且4个放牧组之间差异不显著。放牧4~12 h组的谷胱甘肽过氧化物酶(GSH-Px)显著(P=0.020)低于舍饲组,但其超氧化物歧化酶(SOD)显著(P<0.001)高于舍饲组。总抗氧化能力(T-AOC)在各组间差异不显著。2)放牧时间与羊肉中的MDA、SOD水平呈二次曲线关系,MDA先降低,至放牧10 h后升高,SOD先升高,至放牧10 h后降低;GSH-Px水平与放牧时间呈负线性关系,放牧时间越长其值越低。3)放牧4,12 h组的PUFA和高度氧化多不饱和脂肪酸(HP-PUFA)均显著高于放牧2 h和舍饲组(P<0.01)。PUFA和HP-PUFA沉积量与抗氧化物浓度之间关系密切。绵羊每天放牧2~10 h,可以改善羊肉的氧化稳定性,防止PUFA氧化,从而延长高品质羊肉的保质期。     

多不饱和脂肪酸的营养作用及其基因表达调控

多不饱和脂肪酸(PUFA)是一种重要的营养物质,是所有细胞膜的重要成分,具有重要的生理功能。本文着重论述了PUFA的营养功能和其调控基因表达的分子机制。     

日粮中不同来源多不饱和脂肪酸对育肥猪生长性能和猪肉脂肪酸组成的影响

选取125日龄,体重为60kg左右的杜×长×大三元去势肥育猪32头,随机分为4组,每组8头,公母各半。对照组添加2%猪油,3个试验组分别添加3%亚麻油、3%鱼油和1.5%亚麻油+1.5%鱼油,研究日粮中不同来源脂肪酸对育肥猪生长性能和猪肉脂肪酸组成的影响。结果表明:1)日粮中添加不同油脂对猪生长性能无显著影响;2)日粮中添加3%亚麻油、3%鱼油以及1.5%亚麻油+1.5%鱼油组,可以使猪肉中的C18∶3分别提高236.9%(P<0.01)、27.1%(P>0.05)和204.9%(P<0.01);C20∶5分别提高142.4%(P<0.01)、732.9%(P<0.01)和360.5%(P<0.01);C22∶5分别提高24.0%(P>0.05)、40.5%(P<0.05)和36.4%(P<0.05);C22∶6分别提高5.6%(P>0.05)、222.2%(P<0.01)和136.9%(P<0.01);总n-3PUFA的含量提高131.2%(P<0.01)、317.2%(P<0.01)和223.0%(P<0.01);3)各试验组猪肉中总n-3PUFA的含量显著提高,n-6/n-3的值显著降低。     

Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats

In the present study, we examined the effects of fish oil supplementation in 3 months old male Wistar rats on changes in plasma and liver lipid metabolism and oxidative stress parameters. Twenty Wistar rats were randomly divided into two groups of ten animals: control group and intervention group, treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid and 30 mg docosahexanoic acid. After intervention, biochemical parameters in plasma [triglycerides (TG), low‐density lipoprotein (LDL), high‐density lipoprotein (HDL) and total cholesterol, urea, creatinine and uric acid], fatty acid (FAs) profile of liver phospholipids and parameters of oxidative stress in liver [activity of catalase, superoxide dismutase and paraoxonase (PON1), concentration of nitrites, lipid peroxidation (LPO), free thiol (SH) groups and lactate dehydrogenase (LDH) izoenzymes were determined. Treatment with fish oil improved FAs profile of liver phospholipids, increasing n‐3 FAs and decreasing n‐6/n‐3 ratio. Significant decrease in plasma TG and LDL concentration, and increase in the level of HDL and uric acid were found in intervention group at the end of the study. Catalase activity, LPO, and nitrites concentration in liver were significantly decreased, after the supplementation, together with elevated PON1 activity. Applied treatment significantly improved plasma lipid profile, liver FAs composition and parameters of oxidative stress in male Wistar rats.     

The effect of different concentrations of linseed oil or fish oil in the maternal diet on the fatty acid composition and oxidative status of sows and piglets

N‐3 polyunsaturated fatty acids (PUFA) are essential for foetal development. Hence, including n‐3 PUFA in the sow diet can be beneficial for reproduction. Both the amount and form (precursor fatty acids vs. long chain PUFA) of supplementation are important in this respect. Furthermore, including n‐3 PUFA in the diet can have negative effects, such as decreased arachidonic acid (ARA) concentration and increased oxidative stress. This study aimed to compare the efficacy to increase eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) concentrations in the piglet, when different concentrations of linseed oil (LO, source of precursor α‐linolenic acid) or fish oil (FO, source of EPA and DHA) were included in the maternal diet. Sows were fed a palm oil diet or a diet including 0.5% or 2% LO or FO from day 45 of gestation until weaning. Linoleic acid (LA) was kept constant in the diets to prevent a decrease in ARA, and all diets were supplemented with α‐tocopherol acetate (150 mg/kg) and organic selenium (0.4 mg/kg) to prevent oxidative stress. Feeding 0.5% LO or 0.5% FO to the sows resulted in comparable EPA concentrations in the 5‐day old piglet liver, but both diets resulted in lower EPA concentrations than when 2% LO was fed. The highest EPA concentration was obtained when 2% FO was fed. The DHA level in the piglet liver could only be increased when FO, but not LO, was fed to the sows. The 2% FO diet had no advantage over the 0.5% FO diet to increase DHA in the piglet. Despite the constant LA concentration in the sow diet, a decrease in ARA could not be avoided when LO or FO were included in the diet. Feeding 2% FO to the sows increased the malondialdehyde concentration (marker for lipid peroxidation) in sow plasma, but not in piglets.     

日粮n-3多不饱和脂肪酸对母猪繁殖性能的影响

n-3多不饱和脂肪酸作为一种重要的营养物质,能影响家畜繁殖性能。本文主要从n-3多不饱和脂肪酸作用机理、使用效果等方面阐述n-3多不饱和脂肪酸对母猪繁殖性能的影响。     

不同n-3/n-6多不饱和脂肪酸对产蛋鸡免疫功能的影响

选用 16 8只 30周龄海兰褐蛋鸡 ,研究不同 n- 3/ n- 6多不饱和脂肪酸 (PUFA)对产蛋鸡免疫功能的影响。 n- 3/ n- 6比值分别为 1∶ 1,1∶ 4 ,1∶ 8,1∶ 16 ,1∶ 32 ,维生素 E添加水平为 30 mg/ kg和 30 0 mg/ kg。在试验开始后的第 5周和第 7周给每个处理组中的 6只鸡注射牛血清白蛋白 (BSA) ,并分别于注射后第 10天翅静脉采血 ,测定 BSA抗体效价。测定第 5周及第 10周时外周全血淋巴细胞增殖速度和蛋黄 Ig Y水平。在第 9周给每个处理中 4只鸡注射脂多糖 (L PS) ,并于注射后 6 h,12 h,2 4 h后采血测定血浆中抗体 Ig G水平。试验结果表明 ,随着日粮中 n- 3/ n- 6比值的下降 ,BSA抗体效价显著下降 (P<0 .0 5 ) ,全血淋巴细胞增殖速度在第 5周时有上升的趋势 ,但差异不显著 ,而在第 10周时显著上升 (P<0 .0 5 )。蛋黄中抗体 Ig Y水平有随 n- 3/ n- 6比值下降而下降的趋势。注射 L PS后各时间点血浆 Ig G浓度随着 n- 3/ n- 6比值下降而下降。当日粮中 n- 3/ n- 6比值在 1∶ 1～ 1∶ 8之间时 ,产蛋鸡具有较好的免疫功能     

Meta分析法研究亚麻籽对蛋黄中多不饱和脂肪酸的影响

为研究蛋鸡日粮中添加亚麻籽对蛋黄中多不饱和脂肪酸富集的影响,根据相关标准,采用人工和系统的检索方法检索相关文章,并用荟萃分析(Meta-analysis)的方法分析计算合并标准均数差(SMD)及95%置信区间(95%CI)。通过检索与筛选,共有8篇文章21个试验被纳入,共包括1390个研究对象。Meta分析的结果显示:蛋黄中亚麻酸(ALA)、二十二碳六烯酸(DHA)、亚油酸(LA)、总n-3多不饱和脂肪酸(n-3PUFA)、花生四烯酸(AA)、总n-6多不饱和脂肪酸(n-6PUFA)、n-6PUFA/n-3PUFA的SMD和95%CI分别为17.51(13.34,21.69)、5.39(4.22,6.55)、0.42(-0.39,1.23)、8.86(7.59,10.14)、-1.62(-2.54,-0.70)、-0.44(-0.97,0.08)和-5.22(-6.62,-3.82);混合线性模型的结果显示:DHA和n-3PUFA的异质性来源为亚麻籽添加剂量,蛋黄中ALA、AA、LA和n-6PUFA/n-3PUFA的异质性来源为亚麻籽的添加剂量和蛋鸡产蛋周龄。由分析结果可知,蛋鸡日粮中添加亚麻籽能显著提高蛋黄中的ALA、DHA及n-3PUFA的含量并能显著降低AA和n-6PUFA/n-3PUFA的水平,且富集水平受到亚麻籽添加剂量和蛋鸡产蛋周龄的影响。     

断奶仔猪氧化应激模型的建立及其营养调控的研究进展

随着养殖业的迅速发展和养猪规模的不断扩大,早期断奶技术在现代化养猪生产中已经普及。仔猪早期断奶会造成仔猪体内产生大量的自由基,引起氧化应激。本文以断奶仔猪氧化应激的产生及危害为切入点,着重阐明仔猪氧化应激模型的构建方式,主要介绍了氧化鱼油、Diquat、H2O2攻毒这三种造模方法,最后从营养调控角度提出添加植物提取物、复合抗氧化剂、微量元素、中草药添加剂等物质对缓解仔猪氧化应激的重要意义,以期为断奶仔猪的营养调控提供一定的理论依据。