Effect of dietary crude protein concentration on semen yield and quality in male broiler breeder fowls

1. The responses in semen volume and spermatozoal concentration of 48 caged male broiler breeder fowls fed on a restricted quantity of food containing 80, 110, 152, 210, 290 or 400 g crude protein (CP)/kg from 18 to 64 weeks of age were studied.

2. The proportion of males producing semen declined with increasing dietary crude protein content and resulted in a significant decrease in total semen volume and spermatozoa production.

3. The relationship between the concentration of spermatozoa in the semen and dietary crude protein was negative but trivial. There were no relationships between dietary crude protein content and semen volume or the metabolic activity of spermatozoa.

4. The metabolic activity of spermatozoa was lower at 25 compared with 37 weeks of age.

5. The proportion of males giving semen peaked between 32 and 42 weeks of age. The relationship between age and semen volume and concentration of spermatozoa was negative and linear.

6. Blood plasma uric acid concentration increased linearly above 106 g CP/kg and males on 400 g CP/kg developed articular gout and had to be destroyed.     

Photoperiod and seasonal influences on the growth of turkey hens

1. The influence of photoperiod and time of year on the growth of hen turkeys was investigated.

2. Photoperiod treatments consisted of computer simulations of day lengths which occur naturally in the spring and autumn of the year. Both photoperiod treatments were given to hens in the autumn and spring.

3. Data were collected for growth evaluations at 2‐ to 4‐week intervals to 16 weeks of age. No significant season × photoperiod interactions occurred.

4. The spring trial resulted in greater body weights and better food conversions (food/gain) than the autumn trial.

5. Simulated autumn daylengths resulted in greater body weights and improved food efficiency at 16 weeks of age compared with simulated spring daylengths.

6. The pattern of growth (period changes) was influenced by season, but not photoperiod.

7. Season and photoperiod both have important and independent influences on the growth of the turkey hen.     

Effects of restricting body weight gain or limiting the photoperiod during rearing on sexual maturity,viability and semen production in large white Turkey males

1. Severe restriction of food from 6 to 54 weeks of age resulted in lower body weights and smaller testes weights in male turkeys.

2. As a proportion, mortality was 0.5 in turkeys fed ad libitum and 0.1 in those which were restricted. There were significantly fewer leg problems in the birds undergoing food restriction.

3. Sexual maturity was delayed 4 weeks by food restriction although semen quality was similar to that in ad libitum‐fed birds.

4. Individual semen production was lower after food restriction but, because mortality and food consumption were also lower, semen production per male housed and semen production per kg of food eaten were proportionally 0.30 and 0.32 greater in restricted‐compared with ad libitum‐fed turkeys.

5. Useable quantities of good quality semen were obtained from ad libitum‐fed turkeys at 23 weeks of age.

6. A short photoperiod (7L:17D) from 10 to 18 weeks of age in ad libitum‐fed turkeys produced more rapid development of normal semen production compared with turkeys given a longer photoperiod (14L:10D).     

Heterophil/lymphocyte ratio as a selection criterion for heat resistance in domestic fowls

1. Two experiments were conducted to examine the Heterophil/Lymphocyte ratio (H/L) as criterion for selection for resistance to heat stress and some aspects of general resistance in domestic fowls.

2. The first experiment on a small sample (n = 64) of light breed Iraqi local fowls showed that this ratio could be used as an indicator of heat stress resistance.

3. The second experiment on a large sample (n= 1160) of heavy parent stock confirmed the results. It showed that there was a different H/L ratio for the two strains and that there was much individual variability in the H/L ratio within‐strain that could be utilised for individual selection for heat resistance.

4. The H/L ratio was highly heritable; Resistant and Sensitive groups, defining all individuals with an H/L ratio over the upper 95% confidence limit as ‘Sensitive’ and those below as ‘Resistant’, produced progeny with significantly different H/L ratios.

5. There were indications of differences in mortality between the progeny of the Resistant and the Sensitive groups.

6. The H/L indicator could be used as a criterion to select for heat stress resistance.     

Effect of restricted and ad libitum feeding on semen production and fertility in broiler breeder males

1. The effect of food restriction on the quality of semen production and fertility in broiler breeder males was studied.

2. Seventy‐two Ross broiler breeder males, from 20 to 54 weeks of age, were divided into 4 groups and fed as follows: group 1 = 110 g/bird/d; group 2 = 120 g/ bird/d; group 3 = 130 g/bird/d; group 4 = ad libitum.

3. Body weight, sperm quality (volume, concentration, % motility and % live cells) and fertility were measured. The birds were slaughtered at 55 weeks of age; the abdominal fat pad and testicles were weighed.

4. Groups 2 and 3 produced the highest volume of semen. The quality of semen was very similar in all the restricted groups.

5. Males fed ad libitum produced semen with the best motility and percentage of live cells. Groups 3 and 4 showed the best fertility percentage (79%) against group 1 and 2 (59 and 72% respectively).     

Long-bone properties and development are affected by caponisation and breed in Polish fowls

1. The aim of the study was to investigate the effects of caponisation on bone development of males of two native breeds in Poland.

2. The weight, length and cross-sectional area of tibiae and femora were measured, densitometric measurements and tests of strength were determined and dimensions were calculated.

3. Breed and caponisation did not influence bone weight and length. Higher mechanical strength of the femur was found in entire males, mainly in the Polbar breed. Tibial strength was reduced in capons of the Green Partridge breed. Maximum elastic strength was greater in the Polbar, irrespective of caponisation. Bone cross-sectional area was influenced by breed, while caponisation reduced femoral bone mineral density in both breeds.

4. Caponisation thus increased growth rate but had adverse effects on bone development.

5. Caponisation had fewer negative effects in the Polbar than in the Greenleg Partridge.     

The effect of continuous aeration on the fertility of fowl and Turkey semen stored above 0 °C

1. Fowl and turkey spermatozoa were found to utilise oxygen when stored in tubes at 5 and 10 °G respectively; the storage medium becoming rapidly anaerobic.

2. This aerobic metabolism was encouraged by continuous introduction of air into the semen during storage.

3. Fertility of turkey semen stored at 10 °C for 24 h in a diluent containing glucose was increased 24‐fold by simple aeration and reached 99% of that achieved with fresh semen.

4. Fertilities of greater than 90% were also achieved with fowl semen stored aerated for 48 h at 5 °C in a diluent with or without glucose. The increased fertility on aeration was greater (2.2‐fold) with fowl semen stored in the absence of glucose.     

The relative growth of internal organs in light and heavy breeds

1. The relative weights of the gastrointestinal segments and of some other internal organs from light and heavy breed chicks from hatching to 21 days of age were compared.

2. The relative weight of the duodenum and jejunum was higher in the light breed than in the heavy one, the reverse relationship was found for the ileum and caecum.

3. The relative growth of the pancreas, heart, cerebrum and cerebellum was greater in the light breed.     

Male-to-female aggression in cage-housed common pheasants (Phasianus colchicus) during the breeding season was not related to male plasma testosterone level

1. The aim of this study was to investigate if male-to-female aggression of common pheasants in the course of the breeding season was related to the concentration of plasma testosterone and/or other biochemical plasma indicators in male pheasants housed in breeding cages. The influence of season on the concentration of testosterone and biochemical indicators was also investigated.

2. Males were divided into non-aggressive and aggressive groups during the breeding season based on ethological evaluation. At the beginning, in the middle and at the end of the breeding season, a blood sample was taken from all males on the same day and the concentration of selected biochemical indicators and the total circulating testosterone in the plasma were determined.

3. Male-to-female aggression during the breeding season of pheasants was not influenced by the total plasma testosterone of males.

4. The concentration of total plasma testosterone in males decreased gradually during the breeding season.

5. Male-to-female aggression of pheasants did not have a significant effect on any of the assessed biochemical indicators.

6. The influence of the breeding season affected the activities of alanine aminotransferase and aspartate aminotransferase as well as the concentrations of glucose, magnesium, potassium and chloride in the blood plasma of cage-housed male pheasants.     

Effects of dietary crude protein content and food intake on the production of semen in two lines of broiler breeder males

1. The effects of dietary crude protein (CP) concentration and feeding rate on semen production and quality from 21 to 66 weeks of age were assessed in a factorial experiment with 48 caged broiler breeder males from 2 strains.

2. The probability of obtaining semen and semen volume were lower in one of the two strains but the concentration and yield of spermatozoa were similar.

3. There was no evidence that males which were fed on an increasing quantity of food produced more semen or a greater concentration of spermatozoa than males on a fixed allocation of 120 g/d food.

4. The concentration of spermatozoa was lower in males fed on a diet containing 160 compared with 120 g CP/kg. On termination of the experiment, average testes size was small and testicular function was absent in a large proportion of the males on the high protein diet.     

Traits influencing the hatching performance of Japanese quail eggs

1. Japanese quail eggs from moderately heavier sires showed superior fertility; while fertile eggs from moderately heavier dams hatched slightly better than the eggs from lighter dams.

2. Higher rates of fertility and hatchability of Japanese quail eggs were observed from parents of 10 to 19 weeks of age, with peak fertility and hatchability at 14 and 12 weeks of age, respectively.

3. Sex ratios of 1:2 to 1:5 gave comparable fertility and hatchability results.

4. The hatching performance of quail eggs from cage and deep litter reared breeders was comparable.

5. Fertility and hatchability were directly proportional to the egg weight.

6. Quail egg shell colour, tints and blotches were found to influence hatching performance.

7. Storing quail eggs at 16 ± 2°C and 75 ± 5% relative humidity for more than 4 d reduced hatchability.

8. Hatchability of eggs stored at room temperature was improved if they were sealed in polyethylene bags.

9. Provision of light during the first 14 d of incubation resulted in a photo‐acceleration of about 3.2 h.     

Agonistic interactions in developed and undeveloped breeds of chickens

1. Agonistic interactions were observed over a 24‐week period in Rhode Island Red and in Nigerian local fowl aged 32 weeks or 12 weeks at the start of the experiments.

2. Agonistic interactions were both more numerous and more severe (fights and peck avoidances) in groups of the local breed than in those of Rhode Island Reds.

3. The outcome of severe interactions favoured local males and Rhode Island females in the first experiment and Rhode Island males in the second.

4. Small body and comb size might account for the relatively high number of agonistic interactions and the submissiveness of local females and young males while physical strength or some other breed characteristic might account for the high number of agonistic interactions of local males as well as their dominance in breed encounters.     

Metabolic and anatomical adaptations of light‐bodied chicks to intermittent feeding

1. Chicks of a light‐bodied strain were deprived of food on alternate days from 15 to 46 d of age.

2. Hyperphagia, amounting to twice the intake of control birds early in the experiment, occurred on the repletion day.

3. Final body weight attained 80% of that of the controls.

4. Body fat concentration decreased at first but returned to that of controls after 3 weeks.

5. A consistent hyperlipaemia was observed after food deprivation or restoration.

6. There was a marked increase in the digestive enzymes found in the small intestine, but not in the pancreas during the repletion day.

7. These responses may be considered as mechanisms to reduce the physiological manifestations of starvation.     

Preservation of fowl semen in liquid nitrogen: Application to breeding programmes

1. A method of freezing semen of individual males was adapted for use under farm conditions using an automated freezing apparatus.

2. An insemination programme to produce high fertility and hatchability with semen which had been deep frozen for 2 months was devised.

3. Over 90% fertile eggs with a 90% hatch of all eggs set was obtained with frozen and thawed semen over a period from the 2nd to the 12th day after the first of four inseminations. The persistency of fertility was also tested and 93, 86.6 and 30.7% of the eggs were fertile during days 2 to 6, 2 to 8 and 9 to 15 after the last insemination.

4. Corresponding with the high fertility rate, chicks were produced by every hen that was inseminated and from every male whose semen was frozen and stored. The implications for future breeding practices of this successful result are discussed.     

Effect of gradual acclimation to high ambient temperatures on egg‐shell quality of the Sinai Bedouin fowl,the commercial white leghorn and their crossbreds

1. The effect of gradual acclimation to high ambient temperatures on egg‐shell quality was studied in the Sinai fowl, the commercial White Leghorn, and their reciprocal crossbreds.

2. The Leghorn egg was characterised by a thinner and weaker shell compared with the Sinai and the crossbreds, at all the experimental temperatures.

3. In contrast to other reports, high ambient temperatures for a long period had only mild effects on egg‐shell quality.

4. The results suggest that gradual acclimation to high ambient temperatures might improve the efficiency of the physiological mechanisms involved in the hen's response to heat. Consequently, the reproductive process adapts to the hot environmental conditions.

5. The results indicate that the Sinai breed might be used for future selection of a breed, highly resistant to extreme environmental conditions and with an improved shell quality.     

Behavioural and growth responses of broiler chickens to dietary water content and climatic variables

1. The behavioural responses and growth of broiler chickens were investigated over three seasons using two wet mashes with food:water ratios of 2:1 (HW) and 3:1 (MW).

2. Wet mashes fed in the hot dry season caused significantly lower rates of panting, a major thermoregulatory behaviour. This may be an indication of increased comfort in hot dry conditions for birds fed wet mashes.

3. Feeding activities of birds fed dry and wet rations respectively increased and decreased with decrease in seasonal temperature. The peaks of both feeding activity and consumption in the cool‐dry season were attained by birds fed dry diets and in the hot dry season by those fed wet mashes.

4. Diurnal distributions of food and water intakes were more even in the hot dry season for those fed wet mashes. This may again suggest a higher degree of comfort on the hot dry season when birds are fed wet mashes.

5. In the hot dry season, 60% of birds preferred wet mashes, whereas 80% of birds preferred dry meal in the cool dry season. This may be attributable to the lower temperature of wet mashes.

6. Similar patterns in feeding activity, food consumption and daily weight gain occurred in response to dietary presentation and season.

7. Feeding of dry meal under cool dry conditions and wet mashes in the hot season may be advantageous. Irrespective of season, water temperature may have to be raised to about 30°C before addition to meals for chicks under brooding. This may be necessary as preliminary investigations in this laboratory indicate that food temperatures below 22°C may at the brooding stage discourage food intake and activities.     

Differences in egg‐shell quality among the Sinai Bedouin fowl,the commercial white leghorn and their crossbreds

1. A comparison of egg‐shell characteristics was made among the Sinai breed (a desert inhabiting strain), the commercial White Leghorn and their reciprocal crossbreds.

2. The Sinai egg was smaller and its shell thicker and stronger than the Leghorn egg. All the differences were statistically significant.

3. Shell thickness and strength of Leghorn eggs were in accordance with values predicted on the basis of egg mass, while those of the Sinai and the two crosses were considerably higher than predicted.

4. The apparent differences in mean egg mass between Sinai and Leghorn breeds did not explain the differences in egg‐shell quality, neither did the shape index which was very similar among all breeds.

5. The findings suggest that the differences in egg‐shell properties are of a genetic origin.

6. The thick, strong and less permeable egg‐shell of the Sinai breed may reflect adaptations to its arid environmental origin and to incubation in the open.

7. These genetically‐determined egg‐shell characteristics might serve in a future selection for improved egg‐shell quality in poultry.     

Inheritance of body weight and rate of gain in Japanese quail

1. The effects of hatch date, location and sex on the body weight and rate of gain in Coturnix coturnix japonica were examined.

2. The data conformed to a mixed model and adjustments were made for the fixed effects sex, hatch date and location. Adjustment improved the precision of the estimates by reducing the error variances significantly.

3. Rearing equal numbers of birds from each family at different locations made the interactions negligible.

4. Weight gain was greater if illumination intensity up to 35 d was less.

5. Females were not heavier on the 1st day but were heavier on the 14th and 35th days than males.

6. Variance components and genetic parameters were estimated.     

Explanted embryo culture: In vitro and in ovo techniques for domestic fowl

1. Embryos of the domestic fowl (72 h old) have been explanted into shell‐less cultures or ‘surrogate’ eggshells, in order to investigate the possibility of rearing these embryos to hatching.

2. Rocking embryo cultures during the first half of incubation enhanced embryo growth.

3. Embryos explanted into ‘surrogate’ eggshells of either other individuals or other species have been successfully ‘hatched’.

4. A normal chorioallantois is formed in these surrogate eggshells. This enables a functional albumen sac to form and eggshell resorption to be achieved.

5. Embryos grown in ‘surrogate’ eggshells are slightly smaller than controls but otherwise normal.

6. The technique provides opportunities for genetic engineering experiments.     

Digestibility studies in broiler chickens: Influence of genotype,age, sex and method of determination

1. The influences of genotype, age and sex on droppings digestibility coefficients of a compound food were studied using male and female broiler chickens of three different genotypes at 2, 4 and 6 weeks of age.

2. Because the traditional method of determination of droppings digestibility coefficients of nitrogen may lead to systematic errors in estimating the feeding value of foodstuffs, a method is proposed to determine the ileal digestibility coefficients. The ileal method is compared with the droppings method for a mixed food and for two foodstuffs: wheat and solvent‐extracted soyabean meal.

3. Birds selected for efficient food conversion showed distinctly higher digestibility coefficients for all nutrients than birds selected for high growth potential or birds from a commercial strain.

4. The influence of age on digestibility coefficients was not consistent.

5. Female birds showed digestibility coefficients which were, in general, 3% higher than those of male chickens.

6. Interactions between genotype and sex and between genotype and age for energy metabolisability were the only interactions observed for digestibility measurements.

7. The method of determination influenced the amino acid digestibility coefficients of the mixed food and the relative feeding values of wheat and soyabean meal.

8. It is important to use well defined animals (genotype, sex, age) in evaluating foodstuffs.

9. The preferred method for determination of digestibility coefficients of nitrogen and amino acids is based on ileal sampling, although the differences in amino acid digestibility coefficients were small between methods.