Effects of diosmin on changes of TNF-α, IL-1β, IL-6, IL-8 and IL-10 in serum and kidney tissues of rats with kidney ischemia and reperfusion

AIM: To observe the effects of diosmin on the production of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), IL-6, IL-8 and IL-10 in serum and kidney tissues of rats with kidney ischemia and reperfusion (I/R). METHODS: Sprague-Dawley rats (180 in total) were randomly divided into 3 groups including sham operation group (sham),I/R group and diosmin+I/R group (diosmin+I/R). At the end of the experiment, the blood and kidney tissues were obtained and TNF-α, IL-1β, IL-6, IL-8 and IL-10 were detected by ELISA. RESULTS: The levels of TNF-α, IL-1β, IL-6, IL-8 and IL-10 in serum and kidney tissues in I/R group and diosmin+I/R group were significantly higher than those in sham group (P<0.01 or P<0.05). Following the development of the pathologic process, the level of TNF-α, IL-1β, IL-6 and IL-8 was significantly increased in I/R group and diosmin+I/R groups, but the level of IL-10 was significantly decreased in I/R group and significantly increased in diosmin+I/R group. The levels of TNF-α, IL-1β, IL-6 and IL-8 in I/R group was significantly higher than those in diosmin+I/R group (except TNF-α at 1 h in diosmin+I/R group). The level of IL-10 in diosmin+I/R group was significantly higher than that in I/R group (P<0.01 or P<0.05). CONCLUSION: Diosmin not only decreases the production of TNF-α, IL-1β, IL-6 and IL-8, but also promotes the production of anti-inflammatory cytokine IL-10, suggesting that the protective effect of diosmin on kidney I/R injury was associated with anti-inflammatory mechanism.     

Association of single nucleotide polymorphisms of IL-1, IL-10 and TNF-β with rheumatoid arthritis in Chinese Han population from Quanzhou

AIM: To explore the genetic characteristics of enrolled rheumatoid arthritis and genetic mechanisms of rheumatoid arthritis (RA) by studying the associations of single nucleotide polymorphisms (SNPs) with rheumatoid arthritis in Chinese Han population from a very high prevalence area of rheumatoid arthritis, Quanzhou. METHODS: A case-control study of 155 rheumatoid arthritis patients (RA group) and 170 normal controls (control group) from Quanzhou were enrolled. All of 5 SNPs were genotyped by allele-specific polymerase chain reaction (PCR) and analyzed by SPSS 19.0. χ²-test was applied to predict Hardy-Weinberg equilibrium, and allele and genotype frequencies between RA group and control group were compared. Logistic regression models were used to analyze SNPs. Link disequilibrium analysis and haplotype analysis were performed with SHEsis software. RESULTS: Total of 1 SNP in control group was confirmed by Hardy-Weinberg equilibrium test (P>0.05), and 1 SNP in RA group was confirmed by Hardy-Weinberg equilibrium test (P>0.05). Allele frequencies of 4 SNPs were significantly different between control group and RA group (P <0.05). CONCLUSION: The SNPs of IL-10 rs1800893, IL-1β rs16944, TNF-β rs2009658 and TNF-β rs1041981 were associated with the incidence of rheumatoid arthritis in Chinese Han population of Quanzhou. Allele G of IL-10 rs1800893, allele G of IL-1β rs16944, allele C of TNF-β rs2009658 and allele C of TNF-β rs1041981 can be used as potential genetic markers for the diagnosis of RA in Quanzhou, Fujian.     

Association of IFN-γ and IL-4 gene polymorphisms with childhood bronchial asthmatic susceptibility and the levels of plasma IFN-γ,IL-4 and IgE

AIM: To investigate the gene polymorphisms of interferon-γ(IFN-γ) and interleukin-4(IL-4) and the association with asthmatic susceptibility and the levels of plasma IFN-γ, IL-4 and IgE of asthmatic children. METHODS: 100 asthmatic children and 122 control children were enrolled the study. The genotypes of IFN-γ gene-179G/T polymorphism, IL-4 gene-33C/T and-589C/T polymorphisms were tested by PCR-RFLP.The genotype of IFN-γ gene +874A/T polymorphism was tested by AS-PCR.The CA repeat polymorphism of IFN-γ gene was detected by capillary electrophoresis technique.The levels of serum IFN-γ, IL-4 and IgE were measured by ELISA. RESULTS: 100 asthmatic children and 122 control children were all GG homozygotes at -179 locus of IFN-γ gene.-179 locus of IFN-γ gene has no mutation. The genotypes and allele frequency of IFN-γ gene +874A/T and CA repeat polymorphisms showed no significant difference between asthmatic children and the control(P>0.05). An association was revealed between IFN-γ gene +874A/T polymorphism and the level of plasma IFN-γ.The level of IFN-γ was lower in AA genotype than in AT genotype(P<0.05). The genotypes and allele frequency of IL-4 gene -33C/T and -589C/T polymorphisms showed significant difference between asthmatic children and the control(P<0.05).The levels of plasma IL-4 and IgE were higher in TT genotype at -33 locus and -589 locus than those in CT genotype, but only -33C/T polymorphism was associated with the level of plasma IL-4(P<0.05). CONCLUSION: The IFN-γ gene +874A/T and CA repeat polymorphisms were not correlated with asthmatic susceptibility, but there is significant correlation between the level of IFN-γ and +874A/T polymorphism. TT genotype of IL-4 gene -33 locus and -589 locus maybe the susceptible genotype of asthma in children, and the -33 locus polymorphism is associated with the level of IL-4.     

Effect of the acupotome therapy and electro-acupuncture on the serum contents of IL-1β, IL-6 and TNF-α in rabbits with knee osteoarthritis

AIM: IL-1β, IL-6 and TNF-α play important roles in emergence of osteoarthritis. This study aims at observing the effect of the acupotome therapy and electro-acupuncture on the cytokines in serum of rabbits with osteoarthritis. METHODS: 52 New Zealand rabbits were divided randomly into the normal group, model group, acupotome group and electro-acupuncture group. Knee osteoarthritis of the model rabbits was made with the straightened immobilization method. The acupotome and electro-acupuncture therapies were applied for three weeks. One week after the treatment the serum was collected, and the changes of IL-1β, IL-6 and TNF-α in serum were detected with RIA. RESULTS: In comparison with the control group, the contents of IL-1β, IL-6 and TNF-α elevated significantly in the knee osteoarthritis model group (P<0.05). Compared to the knee osteoarthritis model group, the contents of the cytokines in acupotome treatment group and electro-acupuncture treatment group decreased significantly (P<0.05). No significant difference between the content of cytokines in the acupotome treatment or electro-acupuncture treatment groups (P<0.05) was observed, also no statistical difference between the acupotome treatment or electro-cupuncture treatment groups and the control group was found. However, the contents of the three cytokines in the acupotome treatment and electro-acupuncture treatment groups were still higher than those in control group. CONCLUSION: The acupotome and electro-acupuncture treatment can decrease the release of IL-1β, IL-6 and TNF-α, indicating that the two therapies play an important role in improvement of the articular cartilage cell injury and function through inhibiting the generation of matrix protease and alleviation of degradation of the cartilage matrix.     

邹洋,梁维依,周金盼,李浩

通过分析《辋川集》及辋川图历代摹本,辋川别业中部分植物的造景手法得出了以下结论:辋川别业有采用四时造景的艺术手法进行植物造景;造景形式多为植物围合建筑;植物选取方面,除季相因素外大多采用当时流行的意向植物,以此为获得更好的诗意栖居体验。     

小麦,平菇,棉花,草菇套种技术

小麦、平菇、棉花、草菇套种的经济效益,相当可观。笔者于1987年用此法在半亩小麦田中套种平菇,其结果:收良种小麦350公斤;鲜平菇(投料2500公斤)3000公斤;平菇采收后移栽营养钵棉,并在棉花行中套种草菇,产皮棉75公斤,油籽150公斤,鲜草菇(投料3750公斤)1688公斤。除去农药、肥料、棉籽壳、薄膜等开支外,按当时市价纯收入达8500元。为了使南方农民共同致富,特将有关技术介绍如下:     

李剑雄1,马媛媛2,苏毅1,徐小云2,史新欣

观赏海棠是特指苹果属具有观赏专用价值的种群,其具有耐寒、抗病和观赏周期长等优点,通过近10余年的引种推广,已得到了社会的广泛认可。目前,观赏海棠繁殖方式是以嫁接繁殖为主,而砧木是嫁接过程中极为重要的一环。海棠砧木品种繁多,综述了我国目前海棠砧木的主要资源分类,充分挖掘和利用海棠砧木资源,对提高我国观赏海棠繁育水平起到重大推动作用。     

三色堇,金盏菊,雏菊,金鱼草新品种选育

三色堇、金盏菊、雏菊、金鱼草新品种选育金波，王月新，刘春（中国农业科学院蔬菜花卉研究所，北京１０００８１）关键词三色堇，金盏菊，雏菊，金鱼草，新品种ＢｒｅｅｄｉｎｇａｎｄＳｅｌｅｃｔｉｏｎｏｆＡｎｎｕａｌａｎｄＢｉｅｎｎｉａｌＯｒｎａｍｅｎｔａｌＰｌ...     

黄瓜,葫芦,苦瓜,番茄反季水培技术

近年来,杭州市郊黄瓜、葫芦、番茄等土传性病害(疫病、病毒病等)严重发生,已经不能在秋季进行反季栽培。“八五”期间,我们采用新研制的浮板毛管水培系统(FCH)栽培夏黄瓜、秋黄瓜、秋葫芦、秋樱桃番茄都取得成功,并获得一季亩产值万元以上好效益,产品很受市场欢迎。该系统已在无锡、上海、杭州、广州、深圳等地推广应用。     

龙香,伏香,秋香,晚香梨抗寒调查报告

龙香、伏香、秋香、晚香梨抗寒调查报告王柏林，孙利（黑龙江省农科院园艺研究·哈尔滨）（黑龙江省建三江国营农场管理局·建三江镇）黑龙江省位于我国东北地区的高寒地带，梨树冻害常有发生，有的品种由于冻害而影响树体的正常生长发育、产量和寿命。１９９４年早春黑龙...     

城乡统筹,森林共建,分类经营,协调发展

长久以来,不少地方追求经济发展,忽视生态建设,城乡绿化割裂,"园林城市"建设模式单一,生态效益差。分析了原因及其带来的影响和威胁,阐述了包含郊区乡村森林城市的城乡一体化建设目标。城乡森林共建,分类经营,减少差别,协调发展。     

立体农业—粮,油,果,菜间作配套技术

搞好立体农业,调整农业生产结构,充分利用现有自然资源,取得最佳经济效益是近年来各级政府以及科研部门非常关注、日益重视的课题。实践证明:立体农业是一种集约型高效益的农业生产结构,在目前人多地少、单位面积经济效益不高的情况下,采取立体种植,能因地制宜地综合运用现有栽培技术,充分利用空间、时间和地力,达到提高单位面积产量,增加经济效益的目的。荣成市蔬菜站于1986——1988年选定了几种较好的粮、油、果、菜立体种植形式进行试验研究,并探索出与之配套的栽培技术,使立     

塑料大棚土温,湿度,气体变化规律

塑料大棚土温、湿度、气体变化规律任平太塑料大棚在蔬菜生产中起着重要作用，由于塑料大棚的透气性差，常使棚内出现高温、高湿状况，必须摸清楚棚内土温、湿度、气体的变化规律。大棚内地温的变化规律；大棚内地温高于露地，地温变化规律和露地相似，土壤温度的各时刻呈...     

缘分五月,执手赏兰 赏兰,赏心,赏兴

正中华文化源远流长。中国的花草、山水和中华文化如同肌肤相连,难舍难割。秋兰兮青青,绿叶兮紫茎。绿叶兮素华,芳菲菲兮袭予。芝兰生幽谷,所以赏兰要静心明性,不为外物所染;不以无人而不芳是一种安于淡泊,乐于恬静的品格。竹有节而无花,梅有花而无叶,松有叶而无香,唯兰独并有之,兰花乃草中尤物,花中至宝,更是国人最爱。古人常以花为友,谓之:梅为清友,菊为静友,兰为禅友。千百年来,多少文人雅士爱     

今夜,木园,倒挂金钟的舞会

难得自己有个小小不成气候的院子,守着几朵闲花看云卷云舒.在上海这个繁忙拥挤的城市,这可能就是我心里一点点自在和满足.院子有个卑微的名字,木园.木园亦为牧原、墓园,来亦于此,往也希望亦于此.春末,今夜,木园舞会.那是我与倒挂金钟的一场华丽的邂逅,一段埋藏心底的记忆.我流连其间,希望与君共舞.     

青,取之于蓝,而青于蓝

正红的枫叶、绿的荷钱、紫的牡丹、粉的桃花、黄的银杏……,大自然中的植物五彩斑斓,美不胜收,植物所呈现的颜色主要由植物色素决定。早在远古时期,人类就已经懂得获取植物色素用于美饰服装和绘制图案。"青,取之于蓝,而青于蓝",青是一种颜色,蓝是指能提取染料的草,提取出的染料称为靛蓝。在合成染料出现以前,靛蓝是世界上应用最早也是最广泛的天然植物染料。发表在《科学进展》(2016年)上的一项研究显