共查询到20条相似文献,搜索用时 15 毫秒
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AIM: To establish a mouse model of upper respiratory tract mucosal immunity dysfunction induced by cold stimulation. METHODS: Mice were stimulated with cold by placing the animals in-20 ℃ environment for 5 min, 10 min, 15 min or 20 min. The secretory immunoglobulin A(SIgA) level and lysozyme activity in the mouse saliva were determined for qualification. RESULTS: The mortality rate was 50% in the group of cold stimulation for 20 min. Compared with the control mice, the SIgA level and lysozyme activity significantly decreased in the group of cold stimulation for 15 min (P<0.05 and P<0.01,respectively). Decreased lysozyme activity was only observed in the group of cold stimulation for 10 min (P<0.05). The data in the group of cold stimulation for 5 min were almost similar to those in control group. CONCLUSION: A mouse model of upper respiratory tract mucosal immunity dysfunction was successfully established by cold stimulation. 相似文献
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HEI Zi-qing LUO Chen-fang LI Shang-rong FU Yong-mei MA Wu-hua LUO Gang-jian 《园艺学报》2006,22(8):1650-1653
AIM:To study the changes of serum levels of thromboxane A2 (TXA2) and prostacyclin (PGI2) in cirrhosis patients during liver transplantation.METHODS:Samples were obtained from 24 cirrhosis patients in end at five time points during liver transplantation.TXA2 and PGI2 level were measured by radioimmunoassay.Arterial and mixed venous blood samples used for blood gas analysis were taken at the same time.Intrapulmonary shunt (Qs/Qt) was calculated according to the standard formula.The hemodynamics parameters including continuous cardiac output index (CI),HR,mean artery blood pressure (MABP),MPAP,CVP,PAWP,SVRI,PVRI were measured during liver transplantation.RESULTS:(1) MABP decreased significantly in the early stage of anhepatic period and neohepatic period.(2) CVP,MPAP and PAWP decreased significantly during anhepatic period.They increased significantly after graft reperfusion and remain the high level.(3) CI declined significantly during anhepatic period and increased at 10 min postreperfusion of new liver.(4) SVRI and PVRI increased during anhepatic period and were higher than baseline level at 15 min after reperfusion.SVRI was lower than baseline level at 30 min after reperfusion.(5) Compared with the baseline level,6-keto-PGF1α and TXB2 increased significantly.Compared with the level before vascular cross-clamping,6-keto-PGF1α decreased during neohepatic period and it had significant difference in statistics at the end of operation.CONCLUSION:Serum levels of TXA2 and PGI2 significantly change during liver transplantation and may affect the system and pulmonary circulation to some extent. 相似文献
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AIM: To investigate changes of the level of reactive oxygen species (ROS),malondialdehyde (MDA),transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor (PDGF) expression in a rat hepatic fibrosis model and the effect of curcumin ,and discuss the mechanism of the prophylactic effect of curcumin on hepa tic fibrosis.METHODS: Rat models of hepatic fibrosis were established by intraperitoneally injection of carbon tetrachloride.Curcumin of 20 mg,10 mg,5mg per 100 gram weight of rat was given to these rats respectively at the same time.Normal,fibrosis model and positive groups were made as controls.After eight weeks,all rats were executed and the blood and liver were kept.Serum level of ROS was tested by chromatometry.Content of MDA in liver homogenate was tested by thiobarbituric acid (TBA) method.Expressions of TGF-β1 and PDGF in liver were detected by immunohistochemical method. RESULTS: Serum level of ROS in fibrotic group increased significantly compared with that of normal group,and which was depressed obviously in curcumin groups(P<0.05).Content of MDA in liver of curcumin group reduced significantly compared with that of fibrotic group (P<0.01).Expressions of TGF-β1 and PDGF in fibrotic group increased significantly compared with those of normal group,which were depressed obviously in curcumin groups (P<0.01).CONCLUSION: Curcumin could inhibit expression of TGF-β1,PDGF and lipid peroxidation in liver.These may be mechanisms of curcumin preventing hepatic fibrosis. 相似文献
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HUANG Yue-hong GUO Qi-lan CHEN Zhi-xin CHEN Yun-xin ZHANG Li-juan WANG Xiao-zhong 《园艺学报》2014,30(8):1501-1531
AIM:To explore the development of hepatic sinusoidal capillarization in the early stage of liver fibrosis induced by carbon tetrachloride (CCl4) in rats. METHODS:Clean SD rats were randomly divided into normal control group (group N, n=6) and liver fibrotic model group (group M, n=32). The rats in group N were intraperitoneal injected with saline and the rats in group M were intraperitoneal injected with CCl4 (2 mL/kg, twice a week for 4 weeks). At the end of the 3rd day and the 1st, 2nd and 4th weeks, all rats were killed and then the samples were collected. The pathological changes in the livers were observed by HE staining and Masson straining. The development of hepatic sinusoidal capillarization was observed by transmission electron microscopy (TEM) and immunohistochemical staining. The cell surface expression of vascular endothelium-associated marker CD31, collagen type Ⅳ (Col IV) and laminin (LN) was determined. RESULTS:HE and Masson staining showed the formation of liver fibrosis after treatment with CCl4 for 4 weeks. TEM showed that the fenestrate diameter of liver sinusoidal endothelial cells (LSECs) grew down, the fenestrate numbers of LSECs were decreased along with the development of liver fibrosis, and the consecutive basement membrane was formed at the end of the experiment. The expression of CD31 was significantly increased along with the development of defenestration, and the expression of Col IV and LN was significantly increased after the treatment with CCl4 for 2 weeks and 4 weeks, respectively. CONCLUSION:The typical hepatic sinusoidal capillarization was detected in the early stage of liver fibrosis, and the deposition of LN in the liver sinusoidal walls was the mainly factor of formation of the consecutive basement membrane. 相似文献
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AIM: To investigate the role of autophagy inhibitor 3-methyladenine(3-MA) in the injury of U251 glioma cells induced by H2O2. METHODS: The following groups in this study were set up: control group, 10 mmol/L 3-MA group, 1 mmol/L H2O2 group and 1 mmol/L H2O2 +10 mmol/L 3-MA group. The viability of U251 cells in each group was detected by MTT assay. Autophagic vacuoles in the cells were observed by staining with MDC. The cells were stained with Hoechst 33342 to determine the chromatin condensation. Cell apoptotic ratio was measured by flow cytometry analysis. RESULTS: Compared with control group, no effect of 3-MA on the viability of U251 cells was observed. In H2O2 group, the cell viability decreased and cell apoptotic ratio increased.The autophagic vacuoles and nuclear chromatin condensation in the cells were also detected. Compared with H2O2 group, addition of 3-MA inhibited the increase in autophagic vacuoles but exacerbated the apoptosis. CONCLUSION: Autophagy inhibitor 3-MA inhibits autophagy partially, but exacerbates apoptosis in U251 cells, indicating that autophagy exerts protective effect in the process of injury in U251 cells induced by H2O2. 相似文献
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CHANG Li ZHANG Bo-ai JIA Yan-jie FU Zhen-qiang SHEN Lei SHI Jin-feng WEN Quan-qing ZHAO Er-yi 《园艺学报》2011,27(1):67-71
AIM: To study the role of P2Y1 receptor in the activation of astrocytes induced by Aβ25-35.METHODS: Astrocytes were isolated and cultured from newborn Wistar rats and divided into control group, Aβ25-35 group, MRS2179(P2Y1receptor inhibitor)+Aβ25-35 group and MRS2179 group by treating the cells with the corresponding reagents. The expression levels of GFAP and P2Y1 were determined by the methods of immunohistochemistry, immunofluorescence and Western blotting.RESULTS: No significant change of the astrocyte numbers in all groups was observed. Compared with the control cells, the fluorescence intensity of GFAP significantly increased in Aβ25-35 group and decreased in both MRS2179+Aβ25-35 group and MRS2179 group. The expression level of GFAP determined by Western blotting and immunofluorescence showed the similar trend of change in each group. Compared with control group, the expression of P2Y1 in Aβ25-35 group was significantly increased (P<0.05), and no significant change between MRS2179+Aβ25-35 group and MRS2179 group was found (P>0.05).CONCLUSION: Aβ25-35 activates astrocytes by activation of P2Y1 receptor. 相似文献
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AIM: To investigate the effects of astragalosides on autophagy and apoptosis of rat cardiomyocytes induced by hydrogenperoxide (H2O2).METHODS: The injury model of H9c2 cells induced by H2O2 was established, and the cells in astragalosides group and rapamycin group were treated with 20 mg/L astragalosides and 0.1 mg/L rapamycin, respectively. The apoptotic rate was detected by flow cytometry. The autophagy was observed by acridine orange staining. Western blot was used to detect the protein levels of p-mTOR, P70S6K, LC3 and caspase-3. RESULTS: Compared with H2O2 group and rapamycin group, the viability of H9c2 cells in astragalosides group was significantly increased (P<0.05). The shape of the H9c2 cells in astragalosides group was complete, the nuclei were stained with yellow-green fluorescence, and the chromatin was distributed evenly. The protein levels of p-mTOR and P70S6K in the H9c2 cells of astragalosides group were significantly increased (P<0.05), whereas the protein levels of LC3, cleaved caspase-3 and caspase-3 in the H9c2 cells of astragalosides group were decreased significantly (P<0.05). CONCLUSION: Astragalosides enhance the viability, inhibit the apoptosis, increase the protein levels of p-mTOR and P70S6K, and decrease the protein levels of LC3, cleaved caspase-3 and caspase-3 in the H2O2-induced rat myocardial H9c2 cells. The mechanism is related to the mTOR signaling pathway. 相似文献
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WANG Hua ZHOU Bin GUO Xing ZHU Jun-ya CHENG Chun ZHU Chang-lai ZHANG Tian-yi 《园艺学报》2011,27(6):1226-1229
AIM: To investigate the changes of cell growth and cytoskeleton in hepatocarcinoma SMMC-7721 cells treated with ginsenoside Rh2.METHODS: Cell viability and apoptosis under the conditions of ginsenoside Rh2 exposure at different concentrations were measured by MTT test and flow cytometry,respectively. The morphological changes of F-actin labeled with FITC-phalloidin were observed under confocal laser scanning microscope. The structures of nuclear matrix-intermediate fibre system were observed under transmission electron microscope (TEM).RESULTS: Rh2 at 40 mg/L for 4 days inhibited the proliferation and induced apoptosis in SMMC-7721 cells more than those in control group, 10 mg/L Rh2 group and 20 mg/L Rh2 group. The F-actin in the cells treated with Rh2 was well-distributed, lined up in order and the number of fibers increased, while those in the control cells were in disorder and punctiform. The results of whole mount TEM indicated that the intermediate fiber was plentiful, well-distributed and interweaved into a regular network in Rh2 treated cells.CONCLUSION: Rh2 effectively inhibits the cell proliferation, increases the cell apoptosis and induces the change of the cytoskeleton alignment in SMMC-7721 cells. 相似文献
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AIM: To study and compare the pathophysiological effects of serum IgA1 from both the patients with IgA nephropathy (IgAN) and healthy controls on human mesangial cells (HMC). METHODS: Serum IgA1 was isolated with Jacalin affinity chromatography and heated to form aggregates (aIgA1). Primary HMC were cultured and passage 3 and passage 4 of the cells were used. Intracellular calcium release was assayed with confocal analysis. Expression of TGF-β mRNA and the content of supernatant fibronectin were tested by RT-PCR and indirect competitive ELISA, respectively. RESULTS: aIgA1 from patients with IgAN was shown to induce release of intracellular calcium, up-regulation of expression of TGF-β mRNA and secretion of fibronectin in HMC in a similar time-dependent manner as aIgA1 from healthy controls, but the effects of the former were much stronger and the durations was much longer (P<0.05, respectively). CONCLUSION: IgA1 from patients with IgAN was shown to be able to stimulate HMC with a stronger biological effects than that from healthy controls. It is suggested that direct interaction between IgA1 from patients with IgAN and HMC may be another mechanism in the pathogenesis of IgAN. 相似文献
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AIM: To investigate the role of PI3K/Akt and JAK2/STAT3 pathways in the protection of sulfur dioxide (SO2) against limb ischemia/reperfusion (I/R)-induced acute lung injury (ALI) in rats. METHODS: ALI was induced by limb I/R in the SD rats. Na2SO3(0.54 mmol/kg, ip)/NaHSO3 (0.18 mmol/kg, ip) as SO2 donor was injected at 20 min before reperfusion. The inhibitors of JAK2/STAT3 and PI3K/Akt pathways, Stattic (3 mg/kg, iv) and LY294002(40 mg/kg, iv), respectively, were injected at 1 h before reperfusion. Peripheral blood and lung tissues were collected for determining the contents of the cytokines, the protein levels of the molecules related to the signaling pathways, apoptosis and histopathologic changes by ELISA, TUNEL and Western blot. RESULTS: Compared with control group, the content of MDA, the activity of MPO, lung coefficient, apoptotic index, cytokine expression, and the protein levels of p-Akt and p-STAT3 in I/R group all increased significantly, and administration of Na2SO3/NaHSO3 attenuated the damage in the lung. Besides, the results of Western blot showed that the rat lung tissues expressed p-STAT3 protein and p-Akt protein. After I/R, the protein levels of p-STAT3 and p-Akt were increased. After using Na2SO3/NaHSO3, p-Akt was increased, but p-STAT3 was decreased (P<0.05). CONCLUSION: Both JAK2/STAT3 and PI3K/Akt pathways are likely involved in the protective effect of SO2 against limb I/R-induced ALI in rats. The activation of JAK2/STAT3 signaling pathway increases I/R injury. Reversely, the activation of PI3K/Akt signaling pathway reduces I/R injury. Besides, JAK2/STAT3 and PI3K/Akt signaling pathways may have crosstalk during I/R-induced ALI and JAK2/STAT3 pathway may have an impact on the P13K/Akt pathway. 相似文献
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AIM: To study the effects of cyproheptadine (Cyp) and anisodamine (Ani) on the changes of intracellular free Ca2+ concentration ([Ca2+]i) induced by tumor necrosis factor (TNFα) in single endothelial cells, and to explore the mechanisms of TNFα mediated shock and antishock actions of Cyp and Ani. METHODS: Human umbilical vein endothelial cell strains (ECV304) were seed in 35 mm tissue culture dish with 2 mL DMEM culture medium. The cultured cells were loaded by Fluo-3/AM. The spatial distribution and the dynamic changes of [Ca2+]i in single endothelial cell was determined by laser scanning confocal microscopy (LSCM). RESULTS: [Ca2+]i in single endothelial cell after stimulation of TNFα rapidly increased in a dose-dependent manner and approached the peak value within 60 seconds, afterwards, decreased and kept above the basal level. The confocal scanning image showed that [Ca2+]i elevation was more obvious in nuclear than in cytoplasma, and decreased slowly. Cyp (3×10-5, 6×10-5 mol/L) and Ani (2×10-5, 4×10-5 mol·L-1) markedly inhibited TNFα (1.2×10-9 mol·L-1)-induced [Ca2+]i elevation. CONCLUSIONS: TNFα markedly induces elevation of [Ca2+]i in single endothelial cell, it may be an important mechanism of TNFα-induced shock and tissue injury. Cyp and Ani obviously suppress TNFα-induced [Ca2+]i elevation, which probably is one of the mechanisms of their antishock effects. 相似文献
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ZHU Ling-qun FAN Ji-ping HUANG Qi-fu SUN Su-lun GAO Ying ZOU Yi-huai HE Li-yun ZHANG Zhuang 《园艺学报》2001,17(12):1229-1231
AIM: The aim of this work is to investigate the protective effects of Ginsenoside Rb1 (Rb1) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons. METHODS: Apoptosis were measured by flow cytometry; Morphological changes and neuronal necrosis were examined under microscope; The leakage of lactic dehydrogenase (LDH) and the product of nitric oxide (NO) were measured. RESULTS: hypoxia/hypoglycemia cultures for 5 h and reoxygenation induced neuronal apoptosis and necrosis,and significantly increased the leakage of LDH and the product of NO. The effects were enhanced with the extending time of reoxygenation. Rb1 could significantly decrease the percentage of neuronal apoptosis and necrosis, and reduce the leakage of LDH and the product of NO. CONCLUSION: Rb1 had an effect of anti-neuronal apoptosis. This effect might be related to the inhibition of the activity of NO synthase and NO production. 相似文献
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Stands of summer cauliflower were grown within polyethylene-covered tunnels along which a temperature gradient was imposed. Two tunnels were maintained at either normal or elevated CO2 concentrations. At the last harvest (88 days from transplanting) no interaction between CO2 and temperature on total biomass was detected. The total dry weight of plants grown at 531 μmol mol−1 CO2 was 34% greater than those grown at 328 μmol mol−1 CO2, whereas a 1 °C rise reduced dry weight by 6%. From serial harvests the radiation conversion coefficient was 2.01 g MJ−1 and 1.42 g MJ−1 at 531 μmol mol−1 CO2and 328 μmol mol−1 CO2, respectively, but was not greatly affected by differences in temperature. No effect of either CO2 or temperature on the canopy light extinction coefficient was detected. The rate of progress towards curd initiation increased to a maximum at 15.5 °C, and declined thereafter. Provided the effect of temperature was accounted for, CO2 enrichment did not affect the time of curd initiation. From serial harvests after curd initiation, the logarithm of curd weight or diameter were negative linear functions of mean temperature from initiation. Increases in curd weight and diameter at 531 compared with 328 μmol mol−1 CO2 were greater at warmer temperatures (27% at 13 °C compared with 47% at 15 °C, 57 days after initiation). Effects of CO2 on curd diameter were less than those on curd dry weight because the curd dry matter content was greater at 531 compared with 328 μmol mol−1 CO2. Thus, the effects of elevated CO2 concentrations on fresh weight based yield parameters of cauliflower were less than the increase in total dry matter production. 相似文献
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AIM: To study the effects of tetrandrine(Tet) and fructose-1, 6-diphosphate(FDP) on the elevated intrasynaptosomal [Ca2+]i induced by excitatory amino acids(EAA). METHODS: A rapid method for preparing synaptosomes was used, and intrasynaptosomal free calcium([Ca2+]i) was measured by using the fluorescent indicator quin-2. RESULTS: L-glutamate(Glu, 100 μmol/L), aspartate(Asp, 100 μmol·L-1), N-methy1-D-aspartate(100 μmol/L) and Glu(50 μmol/L) plus Asp(50 μmol/L) all elevated intrasynaptosomal [Ca2+]i in a dose-dependent manner. Pretreatment with Tet(10, 30, 60 μmol/L), FDP(15, 30, 75, 150 μmol/L), MK-801(10, 20 μmol/L) and Tet(15, 30 μmol/L) plus FDP(15, 30 μmol/L) all attenuated the increase in intrasynaptosomal [Ca2+]i induced by EAAs mentioned as above in a dose-dependent manner, and the effect of Tet plus FDP was most significant. CONCLUSION: Both Tet and FDP inhibited a rise in intrasynaptosomal [Ca2+]i induced by EAAs, which may be one of mechanisms that Tet and FDP pretect cerebral tissues against ischemia injury. 相似文献
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AIM: To explore the different inhibitory effect of arsenic trioxide (As2O3) on hepatocarcinoma cell growth in SMMC-7721 and BEL-7402 cell lines and its mechanism. METHODS: The cell culture and trypan blue staining were used to study the inhibitory effect of arsenic trioxide on cell growth, and the glutathione (GSH) contents in hepatocarcinoma cells treated with arsenic trioxide were detected. RESULTS: Arsenic trioxide inhibited the growth of BEL-7402 cells in a time and dose-dependent manner. The inhibitory effect was significant at a lower dose of 0.50 μmol/L for 24 h, however, to SMMC-7721 cells, a higher dose of 2.00 μmol/L for 96 h was needed. The inhibitory rate of arsenic trioxide (0.25-2.00 μmol/L) on BEL-7402 cell growth was higher than that on SMMC-7721 cells. The content of GSH in SMMC-7721 cells was much higher than that in BEL-7402 cells . CONCLUSION: There was a significant difference in inhibition of hepatocarcinoma cell growth by arsenic trioxide between BEL-7402 and SMMC-7721 cell lines, the cause of which may be due to the difference in GSH content in BEL-7402 and SMMC-7721 cells. 相似文献
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WANG Kui DU Xiu-lian CHANG Yan-zhong LI Rong-chang SZETO Ke Yee Katie SUN Hong-zhe QIAN Zhong-ming 《园艺学报》2003,19(7):902-904
AIM:To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb2 (Yb2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb2 on proliferation of U-87 MG cells.METHODS:Cell culture and ICP-MS measurement of Yb2.RESULTS:Yb2Tf uptake by U-87 MG cells increased with the concentrations of Yb2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 μmol/L. Also, Yb2 uptake by the cells increased with increase of the mole ratio (Yb2: apoTf), reaching a maximum at 1.5 mole ratio. Yb2Tf in 0.4 μmol/L significantly inhibited proliferation of U-87 MG cells, however, 10 μmol/L Yb3+ had no significant effect on proliferation of the cells.CONCLUSION:The uptake of Yb2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb2 could significantly inhibit proliferation of U-87 MG cells. 相似文献
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AIM: To study the changes of serum autoantibodies against β1-adrenergic and M2-muscarinic receptors in obstructive sleep apnea syndrome (OSAS) patients with chronic obstructive pulmonary disease (COPD), that is, overlap syndrome (OS). METHOD: Serum autoantibodies against β1 and M2 receptors in 26 cases with OS, 32 with OSAS, 30 with COPD and 28 normal subjects were determined by using enzyme-linked immunosorbent assay(ELISA). RESULTS: The positive rates and titer of β1 and M2 receptor autoantibodies are significantly increased in OS group (92.2%,57.7% and 1:98, 1:67), compared to OSAS (71.9%, 40.6% and 1:83, 1:30) or COPD group (70.0%, 36.7% and 1:79, 1:28) (P<0.05), and they are higher in these groups than in the control (25.0%, 14.3% and 1:20, 1:20) (P<0.01). CONCLUSION: Serum β1-and M2 receptor autoantibodies are significantly increased in the patients with COPD, OSAS or OS, compared to the control, and the highest is in OS. 相似文献