春丝1号青皮丝瓜

春丝1号母本99-1-10是1999年从江西农家品种节节长丝瓜中发现的优良特异单株经连续4代自交定向选育成的稳定自交系，叶片中等大小，茎粗壮，生长势强，后期不早衰；第1雌花节位第5-6节，以后每节着生雌花；瓜长圆柱形，纵径30-35cm，横径6-7cm，瓜皮绿色，肉质柔软多汁，口感好；喜湿耐肥，抗丝瓜病毒病。父本2000-1是浙江农家品种中长丝瓜经3代连续自交定向选育成的稳定白交系，叶片中等大小，生长势中等，后期不甲衰，第1雌花节化第8-9节，以后80％-90％节位着生雌花；瓜妊圆柱形，纵径50-55cm，横径2．5-3．0cm，瓜皮绿色，肉质柔软，口感好；喜湿耐肥。     

翠绿早丝瓜新品种的选育

从地方品种湘潭肉丝瓜中选出优良自交系02，从引入丝瓜资源中选出自交系03，杂交配制成一代杂种翠绿早丝瓜。该品种生长势强，较抗丝瓜霜霉病和白粉病；早熟，第1雌花节位7-8节；瓜条棒形、匀直，表皮青绿色，果肉脆嫩、味甜；果长26-28cm，粗6-7cm，单果重约450g，露地栽培每667m^2产量3000kg左右。     

绿苦瓜新品种衡杂苦瓜2号的选育

衡杂苦瓜 2号母本B06是广东一地方品种用双株选择法经 5代自交分离选育的自交系,父本B02-5是湖南地方品种蓝山苦瓜的变异株经 5代自交定向选育而成的优良自交系。该一代杂种中早熟,植株生长势强,主蔓雌花率高。果实长圆锥形,瓜色油绿有光泽,大直瘤,果纵径 25 ~30cm,横径 5 ~6cm,一般单果质量 500g左右,每 667m2 产量 3 000kg左右,高抗病毒病、枯萎病,中抗霜霉病。     

辣椒新品种驻椒18的选育

驻椒18是以从河南南阳农家品种124牛角椒经多代自交选育出的优良自交系驻03为母本,以从茄门甜椒经多代自交选育出的优良自交系驻07为父本配制而成的辣椒一代杂种。全生育期190d(天),春季露地定植至始收68d(天),早熟。植株生长势中等,平均株高56.62cm,开展度63.18cm,第1雌花平均着生节位为第8.43节。果实牛角形,青熟果绿色;果面光滑,纵径14.53cm,横径4.53cm,果肉厚0.33cm,心室数2～4个;味微辣,VC含量1690mg·kg-1,蛋白质1.63%,可溶性糖2.97%,粗纤维2.36%;平均单果质量80.93g,平均单株结果数20.77个,产量3600kg·(667m2)-1左右。田间调查对病毒病、疫病和炭疽病的抗性优于对照墨秀301。适宜在河南省及周边地区早春保护地和露地栽培。     

丝瓜新品种兴蔬皱佳的选育

兴蔬皱佳丝瓜是以四川地方品种合川伏皱通过高代定向选择的自交系S-5-11-2为母本,以兴蔬早佳丝瓜通过三代株内自交、二代系内自交和一代株内自交选择而成的自交系SF0102-5为父本配制的丝瓜一代杂种;该品种生长势中等,早熟,第一雌花节位8-10节,主蔓结瓜为主,节成性好,耐肥水,果实棒形、绿色,横皱明显,不易裂果,商品...     

雅绿2号丝瓜的选育

雅绿 2号丝瓜是以从广东地方品种雅岗丝瓜中经多代筛选出的优良自交系雅 5 1 2 3 0为母本 ,用春丝瓜材料S 8的多代自交后代S8 1 2 10 10 10作父本配制成的一代杂种。该品种早熟 ,以主蔓结果为主 ,第 1雌花节位第 8.6节 ,果实长棒形 ,长 5 4.5 1cm ,横径 4.8cm ,单瓜质量 0 .36kg ,果色绿 ,棱墨绿 ,沟浅 ,瓜条匀称 ,味清甜。适宜广东省春、秋种植 ,一般每 6 6 7m2 产量 2 0 0 0～ 2 5 0 0kg。     

丝瓜新品种早优3号的选育

早优3号是以S-03-8为母本,以S-03-98为父本配制而成的早熟丝瓜新品种。植株蔓生,生长势强,第1雌花节位为第6~8节,节成性好,连续坐果能力强。果实短棒形,纵径23cm左右,横径5.5cm左右,果皮绿色,较粗糙,花痕较大,不易老化,果肉致密,味微甜,细嫩爽口。抗逆性强,平均单果质量476g,每667m2产量3000kg左右。     

西葫芦新品种‘露玉33’的选育

西葫芦新品种‘露玉33’是以国外西葫芦品种的分离后代经多代自交选育而成的纯合自交系‘07-108’为母本,‘06-558’为父本杂交育成的早熟1代杂交种。该品种属矮生密植类型,早熟,从播种到采收40 d,嫩瓜单果质量250 g左右;第1雌花节位6~7节,嫩瓜皮色为鲜嫩的浅绿色,长筒形,光泽度好,商品嫩瓜纵径23.6 cm,横径7.8 cm;植株生长势强,雌花多,抗病,丰产;早春大棚栽培667 m~2产量5 000 kg左右。适合我国北方大部分地区早春各种保护地及露地栽培。     

丝瓜新品种早佳丝瓜的选育

早佳丝瓜是以自交系X02为母本,以自交系H5为父本配制而成的极早熟丝瓜一代杂种.植株生长势较强,早春栽培主蔓第1雌花节位为第6～9节,定植至始收58～62 d(天),全生育期120 d(天)左右;果实长棒圆筒形,纵径32.2～40.4 cm,横径4.5～5.8 cm,果皮绿色,单瓜质量0.82～1.10 kg,VC含量...     

西葫芦新品种京葫12号

京葫12号母本02-1-5-3-10-6-5是法国纤手2号和俄罗斯地方品种杂交后经6代自交系统选育而成的稳定自交系,父本03-3-10-9-8-2-16是从法国Teizer公司的杂交种中经连续6代自交分离定向选择而成的稳定自交系。2005年配制杂交组     

Effects of radiation from 188Re on smooth muscle cell proliferation

AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β^-particles emission from ¹⁸⁸Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by ¹⁸⁸Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [³H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of ¹⁸⁸Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from ¹⁸⁸ Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G₀/G₁ arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation.     

Effect of L-Arg on plasma content of endothelin and expression of c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy

AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.     

Ergebnisse der Leistungsprüfungen der Süßkirschensorte ‚Stella‘ auf Gisela- und Weiroot-Unterlagen in Bulgarien

Zusammenfassung Die Leistungsprüfungen wurden im Zeitraum 1997 bis 2003 mit den Unterlagen Gisela 4 und 5, den Klonnummern 195/20 und 497/8 aus der Gisela-Serie sowie Weiroot 10, 13, 53, 72 und 158 durchgeführt. Dabei dienten Sämlinge von P1 (bulgarische Selektion aus Prunus mahaleb) als Kontrolle. Alle Unterlagen waren mit der Sorte Stella veredelt und im Dezember 1996 in der Versuchsanlage der Agraruniversität in Plovdiv, Bulgarien, im Abstand von 6 m×4,5 m gepflanzt worden. Dabei erfolgte ein Pflanzschnitt. Nach Abschluss der natürlichen Kronenentwicklung wurde jedes Jahr ein Winterschnitt vorgenommen. Der Boden wurde durch mechanische Bearbeitung offen gehalten und nach dem 4. Standjahr wurden die Baumstreifen mit Herbiziden behandelt. Die Wasserversorgung erfolgte durch eine dem natürlichen Gefälle folgende Überflutung, allerdings nicht immer zum optimalen Zeitpunkt, da keine eigene Wasserquelle zur Verfügung stand.Basierend auf den Ergebnissen bis zum Anfang des 7. Standjahres können die untersuchten Unterlagen in zwei Gruppen differenziert werden: starkwüchsig—Weiroot 10, P1 und Weiroot 13; mittelstarkwachsend bis schwachwüchsig—Gi 497/8, Gisela 4, Weiroot 53, Weiroot 158, Gi 195/20, Weiroot 72 und Gisela 5. Letztere zeichnete sich durch besondere Schwachwüchsigkeit aus. Die meisten Wurzelschosser bildeten Gisela 4, Weiroot 10 und Weiroot 13. Weiroot 53, Weiroot 72 und Weiroot 158 entwickelten deutlich weniger und P1, Gisela 5, Gi 195/20 sowie Gi 497/8 keine Wurzelschosser. Den frühesten Blühbeginn induzierte Gisela 4. Die anderen Unterlagen führten, in Abhängigkeit von den Temperaturbedingungen des jeweiligen Jahres, zu einer Verspätung der Blüte: P1 und Weiroot 10 um 1–2 Tage; Gi 497/8, Weiroot 13 und Weiroot 158 um 2–4 Tage; Weiroot 72 um 2–7 Tage; Gi 195/20 um 3–6 Tage; Weiroot 53 um 3–8 Tage und Gisela 5 um 3–10 Tage. Die Reifezeit der Früchte war bei den Bäumen auf Gisela 5 im Vergleich zu den anderen Varianten um 2–3 Tage verspätet. Gisela 5, Weiroot 72 und Gisela 4 induzierten bei der aufveredelten Sorte die höchsten Ertragsleistungen, P1 die geringsten. Bei den Bäumen auf Gisela 5 war die Fruchtgröße geringer als bei den anderen Unterlagen. Bäume auf Gisela 5 brauchen intensive Pflege. Nur wenn alle Produktionsfaktoren und kulturtechnischen Maßnahmen optimiert werden, kann das hohe Ertragspotenzial dieser Unterlage ausgeschöpft werden.     

多效唑对猕猴桃离体试管苗生长及内源激素的影响

多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。     

Proteomic analysis between pathological scars and normal skin

AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars.     

Compositional and Functional Properties of Saskatoon Berry and Blueberry

Abstract

Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared.     

Cryopreservation of shoot apices of hawthorn in vitro cultures originating from East Asia

The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species.     

Coupling past management practice and historic landscape change on John F. Kennedy Space Center,Florida

Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere.     

XBP-01 accelerated apoptosis induced by oxidized low density lipoprotein in vascular smooth muscle cells

AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis.     

Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells

AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [³-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10^-6-10^-4 mmol/L) stimulated [³-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [³-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P＜0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P＜0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10^-6-10^-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P＜0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl₂ for 6 h induced an increase cellular MT content by 5.7-fold (P＜0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P＜0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation.