The influence of grass and maize silage quality on apparent diet digestibility, metabolizable energy concentration and intake of finishing beef cattle

The effects of offering a range of grass silages and mixtures of grass and maize silages on the intake of beef cattle were studied. Four grass silages (GS1, GS2, GS3 and GS4) were used. Grass silage 1 was ensiled from a second regrowth in mid‐late September and treated with an inoculant additive. Grass silages 2, 3 and 4 were ensiled, without additive, from a primary regrowth harvested in early July, late May and mid‐June respectively. Wilting periods were 8, 30, 36 and 36 h for GS1, GS2, GS3 and GS4 respectively. Grass silages 1, 2 and 3 were precision chopped and ensiled in bunker silos, while GS4 was ensiled in round bales. The DM content (g kg^?1) and starch concentration (g kg^?1 DM) of the three maize silages (MS1, MS2 and MS3) used were 256 and 128, 256 and 184, and 402 and 328 for MS1, MS2 and MS3 respectively. Seventy‐two Charolais and Limousin cross‐bred steers were used in a changeover design with two 4‐week periods. The study consisted of sixteen treatments incorporating the four grass silages fed alone and with the three maize silages arranged as a 4 × 4 factorial design. The grass silage and maize silage mixtures were offered in a ratio of 0·60:0·40 (DM basis) once daily using individual Calan gates. All silages were offered ad libitum with 3 kg per head per day of a concentrate supplement. Dry matter and metabolizable energy (ME) intakes were highest with diets based on grass silage GS4 compared with diets containing the other grass silages. Metabolizable energy intakes of diets containing no maize silage, and those based on MS1 and MS2, were similar (P > 0·05) but lower than that of diets containing MS3. Only limited increases were found in DM and ME intakes with the inclusion of maize silage in grass silage‐based diets while offering high‐quality grass silage (assessed in terms of DM content, and fibre and N concentrations) promoted high voluntary intakes.     

A new sterilization and inoculation method in silage research

The study aimed at evaluating an effective sterilization–inoculation technique to facilitate silage research on the effect of forage microflora on fermentation variables. The sterilization effect of heating at 60°C for 3 h + 103°C for 15 h was tested on samples of grass, grass–clover, white clover and maize, pre‐dried at 60°C to a dry‐matter (DM) content >900 g kg^?1. The ensilability of treated samples, reconstituted to original DM concentration (250–390 g kg^?1), was assessed by inoculation with microfloras extracted from the original samples. Microfloral inoculants were obtained by a combination of centrifugation (15 500  g for 40 min) and filtration (0·45 and 0·22 μm pore sizes) of the supernatant. The sterilization treatment effectively sterilized the forage samples but decreased water soluble carbohydrates by 49% and N buffer solubility by 22% and increased the acid detergent insoluble N proportion of total N by 53% (P < 0·05). The reconstituted silages had 18% less lactic acid, 20% less ethanol and 37% less ammonia‐N (P < 0·05), but volatile fatty acids and 2,3‐butanediol did not differ from the untreated silages (P > 0·05). Counts of lactic acid bacteria, enterobacteria, clostridia, yeasts and moulds in the two silage treatments were also similar (P > 0·05). It is concluded that, despite causing chemical and physical alterations, the sterilization–inoculation technique evaluated could be a useful tool for future studies on the effects of microflora on ensiling results.     

The effect of wilting and silage additives on the fermentation of autumn made grass silage ensiled in bunkers on commercial farms in South Wales 1983-85

Silage making practices in respect of 130 samples of autumn made grass silages ensiled in bunkers on commercial farms in South Wales during 1983-1985 were analysed to discern the effect of wilting and/or silage additives on fermentation. Silages were primarily made during late September and early October in fine weather from perennial ryegrass pasture which had not been grazed for 6 weeks. On average 13.9 ha of pasture was cut for silage. Analysis of 120 samples of grass showed it contained 176 g kg^?1 dry matter (DM) with (g kg^?1 DM) 215 protein, 240 modified acid detergent fibre (MADF), 78 water-soluble carbohydrate (WSC) and 95 ash. Most farmers attempted to pre-wilt grass for 1 day prior to ensilage and one quarter of them made unwilted silage. Silage making was usually completed within 6 days of starting to cut and was generally made with a precision-chop machine. Silage additives applied were (kg t^?1) formic acid (4·7), formic acid 4-formalin (5·5), sulphuric acid-+ formalin (5·0) sugars (14) and inoculants (0·65). Formic acid significantly reduced pH, and formic acid with or without formalin significantly reduced ammonia nitrogen (N) content of silages compared with other treatments. Protein contents of acid/formalin treated silages were significantly higher and MADF of acid with or without formalin treated silages were significantly lower than other treatments. Pre-wilting grass prior to ensilage did not significantly increase subsequent silage DM content and significantly increased the pH of non-additive treated silages. Unwilted silages treated with formic acid with or without formalin had a significantly lower ammonia-N content and higher residual WSC than other treatments. It is suggested that only formic acid application either alone or in conjunction with formalin to unwilted silage was successful in producing well preserved silages and that a grass WSC content of 17 g kg^?1 would be necessary to achieve this.     

Effects of the proportions of high or medium digestibility grass silage and concentrates in the diet of beef cattle on liveweight gain, carcass composition and fatty acid composition of muscle

Abstract An experiment was carried out over 2 years to evaluate the effects of increasing the proportion of cereal‐based concentrates in diets containing high‐digestibility and conventional medium‐digestibility grass silages on the dry‐matter (DM) intake, liveweight gain and carcass composition of beef cattle, and to examine the effects of grazed grass and the ratio of grass silage:concentrates in the diet on the fatty acid composition of selected muscle tissues. Late‐maturing steers (n = 231) were offered diets based on high‐digestibility (HD) (0·743 digestible organic matter (DOM) in DM) or medium‐digestibility (MD) (0·643 DOM in DM) grass silages supplemented with barley/soyabean meal‐based concentrates. The concentrates constituted 0·20, 0·40, 0·60 and 0·80 of total DM of the diets, which were offered ad libitum (AL). The two diets, which contained 0·80 concentrates, were also offered at 0·80 of AL intake. A further group of fourteen animals were given the medium‐digestibility silage only for 5 months and then grazed perennial ryegrass pastures for a further 5 months (silage/pasture treatment). For the diets containing HD silage and 0·20, 0·40, 0·60 and 0·80 concentrate, and 0·80 concentrate at 0·8 of AL intake, the DM intakes were 9·4, 10·2, 10·4, 10·2 and 8·1 (s.e. 0·16) kg d^?1, respectively, and daily carcass gains were 0·67, 0·78, 0·77, 0·79 and 0·62 (s.e. 0·029) kg d^?1, respectively; for those containing MD silage and 0·20, 0·40, 0·60 and 0·80 concentrate, and 0·80 concentrate at 0·8 of AL, the DM intakes were 8·2, 9·3, 10·1, 10·1 and 8·0 (s.e. 0·16) kg d^?1, respectively, and daily carcass gains were 0·38, 0·48, 0·64, 0·77 and 0·56 (s.e. 0·029) kg d^?1 respectively. Increasing the proportion of concentrates in silage‐based diets decreased the concentration of omega‐3 (ω‐3) polyunsaturated fatty acids (PUFA) (P < 0·001) and increased the concentration of ω‐6 PUFA (P < 0·001) in muscle. Cattle on the silage/pasture treatment had the highest concentration of ω‐3 PUFA in muscle (51 g kg^?1 lipid), this value being over three times that for animals given diets containing MD silage and 0·80 concentrate in the diet. These results demonstrate the potential of HD silage made from perennial ryegrass relative to high concentrate diets. The consumption of pasture‐finished beef could make a significant contribution towards increasing the intake of ω‐3 PUFA in the human diet.     

Effects of feeding clover silages on feed intake, milk production and digestion in dairy cows

Silages were made from pure crops of perennial ryegrass, red clover and white clover over 2 years. In all but one case the silage was stored as bales. A silage additive specially adapted for bales (Kofasil Ultra^TM) was used for all silages and they were all of good hygienic quality. The additive contained sodium nitrite, hexamethylene, tetraamine sodium bensoate and sodium propionate. The silages were offered ad libitum, either pure or mixed [grass/clover 0·50/0·50 on a dry‐matter (DM) basis] with a fixed amount (8 kg) of concentrate. Two experiments, one in each year, were performed with high‐yielding multiparous dairy cows in mid‐lactation, and both rumen‐cannulated and intact cows were used. The experiments were carried out using an incomplete changeover design with fifteen cows and five treatments each year. The cows consumed large quantities of these silages (12·7–16·3 kg DM per cow per day). The highest intakes were obtained when the red clover and the 0·50 red clover:0·50 perennial ryegrass silage diets were offered. However, there was a difference between years. In year 1, 0·50 red clover:0·50 perennial ryegrass and 0·50 white clover:0·50 perennial ryegrass silage diets showed the highest intakes while pure perennial ryegrass and white clover silage diets gave lower intakes. In year 2 the highest intake of silage was obtained when the diet containing silage from red clover from a second cut was offered, while the silage from red clover from a first cut gave the lowest intake. The voluntary intakes of silages from white clover and perennial ryegrass were intermediate. No cases of bloat or other digestive disturbances were observed. Milk yield was significantly lower for the perennial ryegrass silage diet compared with all other diets in year 1. In year 2 milk yield was highest for the white clover silage diets and lowest for the red clover silage diets from both cuts. In year 1 there were relatively small differences in milk composition while in year 2 milk fat content was significantly lower with white clover silage diet and milk protein content was significantly higher with the perennial ryegrass diet. The overall conclusion from these experiments was that cows were able to consume large quantities of pure legume silage without serious disturbance to their metabolism. Differences in measurements of rumen metabolism were found between diets and especially between years. Milk production differences appears to be coupled to both differences in rumen physical characteristics, such as passage rate and particle size as well as differences in volatile fatty acid production in the rumen.     

The effect of stage of perennial ryegrass maturity at harvesting, fermentation characteristics and concentrate supplementation, on the quality and intake of grass silage by beef cattle

Abstract Two experiments were carried out in consecutive years to examine the influence of cutting date and restricting fermentation by carboxylic acid treatment on the nutrient intake from grass silage by beef cattle. In year 1, four cutting dates during July and August after a primary growth harvest and, in year 2, five cutting dates of primary growth between mid‐May and early July were examined. Herbage was ensiled either untreated or treated with high levels of acid additive (‘Maxgrass’, mean 8·6 l t^?1). Ninety‐six (year 1) or forty‐eight (year 2) continental cross steers were used in partially balanced changeover design experiments with each silage type either unsupplemented or supplemented with 4·5 (year 1) or 5·5 (year 2) kg concentrates head^?1 d^?1. Silage digestibility declined significantly between initial and final harvest dates (P < 0·001), whereas silage dry‐matter (DM) and digestible energy (DE) intakes were significantly higher in the initial compared with final harvest dates in both years of the study (P < 0·01). Similarly, silage DM and DE intakes, and total DM intakes, of acid‐treated and unsupplemented silages were greater than those of untreated and concentrate supplemented silages, respectively (P < 0·001). The results indicate that earlier cutting dates, and addition of acid to herbage before ensiling, can increase silage DM intake by beef cattle.     

Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performance

An Italian ryegrass and hybrid ryegrass sward was harvested on 11 May 1994. The mean dry‐matter (DM) content of the herbage was 197 g kg^–1 fresh matter (FM), and mean nitrogen and water‐soluble carbohydrate contents were 20 and 272 g kg^–1 DM respectively. Approximately 72% of total nitrogen (TN) was in the form of protein‐nitrogen. The herbage was treated with either no additive, formic acid (3·3 l t^–1) (Add‐F, BP) or inoculant (2·3 l t^–1) (Live‐system, Genus) and ensiled in 100 t silos. Changes in effluent composition with time showed that silage fermentation and protein breakdown were delayed by treatment with formic acid. Formic acid and inoculant treatments also inhibited amino acid catabolism during ensilage. All silages were well fermented at opening with pH values < 4·0 and ammonia‐N concentrations of ≤ 50 g kg^–1 TN after 120 d ensilage. Treatment had an effect on protein breakdown as measured by free amino acid concentration, with values of 21·5, 18·2 and 13·2 mol kg^–1 N at opening (191 d) for untreated, formic acid‐treated and inoculated silages respectively. Amino acid catabolism occurred to the greatest extent in untreated silages with significant decreases in glutamic acid, lysine and arginine, and increases in gamma amino butyric acid and ornithine. The silages were offered ad libitum without concentrate supplementation to thirty‐six Charolais beef steers for a period of 69 d (mean live weight 401 kg). Silage dry‐matter intakes and liveweight gains were significantly (P < 0·05) higher on the treated silages. Silage dry‐matter intakes were 7·42, 8·41 and 8·23 kg d^–1 (s.e.d. 0·27) with liveweight gains of 0·66, 0·94 and 0·89 kg d^–1 (s.e.d. 0·058) for untreated, formic acid‐treated and inoculated silage‐fed cattle respectively. In conclusion, additives increased the intake of silage and liveweight gain by the beef steers, and it is suggested that this may be caused in part by the amino acid balance in these silages.     

Silage quality when Moringa oleifera is ensiled in mixtures with Elephant grass,sugar cane and molasses

Fourteen different silages were prepared using mixtures of Moringa (Moringa oleifera), Elephant grass (Pennisetum purpureum cv Taiwan) or sugar cane (Saccharum officinarum). Molasses from sugar cane was used in the amounts of either 10 or 50 g kg^?1 fresh matter (FM) in treatments without sugar cane. A completely randomized design with three replicates of each treatment was used. The silages were prepared in 1800 mL micro silos and opened after 120 d. The presence of Moringa and Elephant grass in the silage changed the pH by ?0·8 and +0·7, respectively (P < 0·001), indicating a favourable effect of Moringa on silage pH. Overall differences were found among treatments for dry matter content, crude protein and acetic acid concentrations, weight loss, CO₂ production and silage pH after spoilage (P < 0·001). Weight loss was proportionately 0·034 and 0·014 in silages with and without sugar cane respectively (P < 0·001). Overall, differences (P < 0·05) were also found for neutral‐detergent fibre and lactic acid concentrations, lactic acid bacteria counts, clostridial counts and time to spoilage of the silages. Treatments containing Moringa had higher lactic acid concentrations (+16 g kg^?1 DM; P < 0·01) compared to treatments without but the presence of Moringa decreased time to spoilage by 67 h (P < 0·05). No differences were found in propionic acid concentration or fungal growth of the silages. It is concluded that Moringa can be used as a component of high quality silages which also contain high concentrations of crude protein.     

Alpha‐tocopherol and β‐carotene in legume–grass mixtures as influenced by wilting,ensiling and type of silage additive

Effects of wilting, ensiling and type of additive on α‐tocopherol and β‐carotene contents in legume–grass mixtures were examined. Swards of birdsfoot trefoil + timothy (Bft + Ti), red clover + timothy (Rc + Ti) and red clover + meadow fescue (Rc + Mf) were harvested as a first regrowth in August 2005. Forage was wilted to a dry‐matter (DM) content of 273 g kg^?1 and ensiled without additive or with an inoculant or acid. Wilting decreased α‐tocopherol concentration by 30% in the Bft + Ti mixture (P = 0·015). Untreated Bft + Ti silage had higher α‐tocopherol content than red clover silages (56·9 vs. 34·2 mg kg^?1 DM; P = 0·015). The α‐tocopherol concentration of Bft + Ti forages increased during ensiling from 41·1 mg kg^?1 DM in wilted herbage to 56·9, 65·2 and 56·8 mg kg^?1 DM in untreated, inoculated and acid‐treated silage respectively (P = 0·015). The inoculant increased α‐tocopherol content in the red clover silages (50·1 vs. 34·2 mg kg^?1 DM; P = 0·015) compared with untreated red clover silages. Red clover mixtures had lower β‐carotene content than Bft + Ti (32·3 vs. 46·2 mg kg^?1 DM; P = 0·016), averaged over treatments. In conclusion, wilting had small effects but the use of bacterial inoculant as an additive and a Bft + Ti mixture increased α‐tocopherol concentration in the silage.     

Factors influencing the response in intake of silage and animal performance after wilting of grass before ensiling: a review

This review examines the response in intake of silage and animal performance after wilting of grass before ensiling. It uses data from eighty‐five published comparisons. Initial analysis identified those variables having the greatest influence on the increase in dry‐matter (DM) intake of grass silage owing to wilting. Total water loss, drying rate of the wilted material and ammonia‐N concentration of the unwilted material were the variables most closely and positively correlated to the proportional increase in DM intake as a result of wilting, with r² values of 0·344, 0·393 and 0·240 respectively. There was little evidence of curvilinearity in any of the relationships. Stepwise regression analysis was used to identify the best multiple linear regressions for predicting the proportional increase in intake owing to wilting. Factors associated with the extent and rate of water loss in the field (total water loss and drying rate) and the quality of the unwilted material ensiled and its subsequent fermentation pattern (DM digestibility, crude protein, ammonia‐N content of unwilted silage) were the key variables in the relationship. Subsequent analysis related the increase in output of milk energy of dairy cows and liveweight gain of beef cattle to the same independent variables. This indicated that variables shown to describe the response in DM intake in the previous analysis were among those most closely related to the increases in animal performance owing to wilting of grass. To assess whether the increase in animal performance owing to wilting could be explained as a direct response to increased metabolizable energy (ME) intake of wilted compared with unwilted silages, linear relationships were derived between the increase in milk and beef production parameters and the increase in ME intake owing to wilting. From these, it was concluded that there was no clear evidence that wilting reduced the efficiency of utilization of ME in beef or dairy cattle. The present study showed that the increase in milk energy output (measured in MJ) per increase in ME intake (measured in MJ) owing to wilting was linear, with a slope of 0·19. This response from additional ME intake owing to wilting is in line with what would be anticipated when increasing ME intake by other methods.     

Characteristics of baled silage made from first and second harvests of wilted and severely wilted forages

First and second harvests of lucerne (Medicago sativa L.), perennial ryegrass (Lolium perenne L.) and a lucerne–perennial ryegrass mixture [80 or 144 g kg^?1 dry matter (DM) of ryegrass] at the first and second harvests were cut and conditioned, wilted to 500 or 700 g DM kg^?1 then baled and stretch‐wrapped for silage on the same dates. Lucerne bales were denser (411 kg m^?3) than bales of perennial ryegrass (331 kg m^?3) (P < 0·05). After an 8‐month storage period, silage made from high DM‐content forage had a higher concentration of neutral‐detergent fibre (NDF) and was less digestible than that made from low DM‐content forage. Daily DM intakes by beef steers, when the silages of the second harvest were fed ad libitum, were 31·2, 31·2 and 22·3 g kg^?1 live weight for lucerne, lucerne–perennial ryegrass mixture and perennial ryegrass silages, respectively (P < 0·01), when the herbage had been wilted to 500 g kg^?1. In vivo digestibility of NDF in the lucerne–perennial ryegrass mixture silage (0·587) was significantly lower than that of perennial ryegrass silage (0·763) but higher than lucerne silage (0·518). Higher intakes of baled lucerne silage tended to offset its lower digestibility values. Lucerne–perennial ryegrass mixture silage had a higher DM and NDF digestibility than lucerne silage, indicating perhaps the presence of associative effects.     

Apparent digestibility and nitrogen utilization of diets based on maize silage harvested at three stages of maturity and fed to steers

Advancing maize crop maturity is associated with changes in ear‐to‐stover ratio which may have consequences for the digestibility of the ensiled crop. The apparent digestibility and nitrogen retention of three diets (Early, Mid and Late) containing maize silages made from maize of advancing harvest date [dry matter (DM) contents of the maize silages were 273, 314 and 367 g kg^?1 for the silages in the Early, Mid and Late diets respectively], together with a protein supplement offered in sufficient quantities to make the diets isonitrogenous, were measured in six Holstein–Friesian steers in an incomplete Latin square design with four periods. Dry‐matter intake of maize silage tended to be least for the Early diet and greatest for the Medium diet (P = 0·182). Apparent digestibility of DM and organic matter did not differ between diets. Apparent digestibility of energy was lowest in the Late diet (P = 0·057) and the metabolizable energy concentrations of the three silages were calculated as 11·0, 11·1 and 10·6 MJ kg^?1 DM for the Early, Medium and Late diets respectively (P = 0·068). No differences were detected between diets in starch digestibility but the number of undamaged grains present in the faeces of animals fed the Late diet was significantly higher than with the Early and Mid diets (P = 0·006). The apparent digestibility of neutral‐detergent fibre of the diets reduced significantly as silage DM content increased (P = 0·012) with a similar trend for the apparent digestibility of acid‐detergent fibre (P = 0·078). Apparent digestibility of nitrogen (N) was similar for the Early and Mid diets, both being greater than the Late diet (P = 0·035). Nitrogen retention did not differ between diets. It was concluded that delaying harvest until the DM content is above 300 g kg^?1 can negatively affect the nutritive value of maize silage in the UK.     

An evaluation of an inoculant/enzyme preparation as an additive for grass silage for dairy cattle

Herbage from the first regrowth of perennial ryegrass-based swards was directly ensiled after treatment with a bacterial inoculant/enzyme preparation (SIL-ALL, Alltech UK) at 3·0 1 t^?1, formic acid (850 g kg^?1) at 2·59 1 t^?1 or no additive (Control). The mean dry matter (DM) and water-soluble carbohydrate concentrations of the grass were 185 and 24·0 g kg^?1 (fresh basis) respectively. Lactic acid concentrations after ensiling increased at a lower rate in formic acid-treated herbage than with the other treatments. All silages were well preserved and formic acid-treated silage had a lower ultimate concentration of lactic acid and higher concentration of water-soluble carbohydrate. Effluent output was increased on a proportional basis by ?0·06 with formic treatment, whereas the inoculant reduced effluent output by 0·05 in comparison with the mean effluent production of the control silage. The in vivo digestibilities of the silages were determined using sheep. The digestibilities of DM, organic matter and energy were significantly higher with inoculant-treated silage than with formic acid treatment, whereas values for the control silage were intermediate. The three silages were offered ad libitum to forty dairy cows with individual recording of daily intakes for a 10-week period in a randomized block experiment with four treatments. Sixteen animals were offered the control silage with half of these offered 3 kg concentrates per day (C3) and the other half offered 7 kg concentrates per day (C7). Twelve animals were allocated to each of the additive-treated silages, with concentrates offered at 5 kg d^?1. Treatment effects on animal performance were measured in weeks 7–10. To compare animal performance for the treated silages with the control, an estimate of performance at 5 kg concentrates per day was obtained by regression using values obtained at 3 and 7 kg concentrates. In comparison with estimated silage intake for the control silage with 5 kg d^?1 concentrates, inoculant and formic acid treatment of the silages increased dry matter intake by 0·04 (P > 0·05) and 0·13 (P > 0·01) respectively. In comparison with estimated milk production and yield of fat plus protein for the control treatment with 5 kg d^?1 concentrates, neither inoculant treatment nor formic acid treatment produced any significant differences.     

The effect of dry feed additives on the nutritive value of silage

Silages were prepared in late September from a mixed crop of perennial ryegrass and white clover with low dry matter and high nitrogen content. Six different treatments were used during ensiling; addition of either molassed sugar beet pulp or rolled barley, at 50 kg and 25 kg (t fresh grass)^?1, formic acid at 51 t^?1 and no additive (control). All silages were well fermented with low levels of ammonia and pH. The addition of rolled barley or sugar beet pulp increased the dry matter content of the silages incrementally and appeared to assist the retention of nitrogen in the silage. Dry matter intakes of silages with 50 kg of barley or sugar beet pulp tonne^?1 were similar to the control silage when fed to wether sheep but at 25 kg t^?1, dry matter intake increased by 0·15 with barley and 0·04 with sugar beet pulp compared to the control. The digestibility of dry matter (DM) and organic matter (OM) were significantly higher than the control for silages treated with formic acid or high levels of barley and sugar beet pulp whereas neutral-detergent fibre (NDF) digestibility did not appear to be significantly affected. All treated silages had a significantly higher metabolizable energy (ME) content than the control, and additions of barley or sugar beet increased the ME content. The ME intakes of all treated silages were also significantly greater than those of the control, and the formic acid-treated and low barley silages were the highest. This appeared to be associated with significantly higher energy digestibility and DM intake. Addition of rolled barley during ensilage resulted in greater nitrogen intake, availability and retention compared to additions of sugar beet pulp. The higher retention with barley silages was associated with a significantly lower proportional loss of absorbed nitrogen in urine, which indicated a more efficient utilization of nitrogen. These differences require further study to determine the mechanisms involved in the interactions between the energy source and herbage protein, both during fermentation and in the rumen.     

Effects of treating low dry-matter grass with a bacterial inoculant on the intake and performance of beef cattle and studies on its mode of action

A randomized block design experiment involving thirty beef cattle (mean initial live weight 462 kg) was carried out to evaluate a bacterial inoculant based on a single strain of Lactobacillus plantarum as a silage additive and to provide further information in relation to its mode of action. Three herbages were harvested on 10 August 1989 using three double-chop forage harvesters from the first regrowth of a perennial ryegrass sward which had received 170 kg N, 25 kg P₂O₅, and 42 kg K₂O ha^?1. They received either no additive (silage C), formic acid at 2·91 (t grass)^?1(silage F) or the inoculant at 3·21 (t grass)^?1 (silage I). Mean dry-matter (DM), water-soluble carbohydrate and crude protein concentrations in the untreated herbages were 158g kg^?1, 88 g (kg DM)^? and 183g (kg DM)^?1 respectively. For silages C, F and I respectively, pH values were 4·01, 3·57 and 3·62; ammonia N concentrations 117, 55 and 77 g (kg total N)^?1; and butyrate concentrations 2·18, 0·50 and l·24g (kg DM)^?1. The silages were offered ad libitum and supplemented with 2·5 kg concentrates per head daily for 77 days. For treatments C, F and I, silage DM intakes were 6·59, 7·25 and 6·80 (s.e. 0·074)kg d^?1; metabolizable energy (ME) intakes 86,99 and 94 (s.e. 0·8) MJ d^?1; liveweight gains 0·90, 0·97 and 1·02(s.e.0·066) kg d^?1; carcass gains 541,656 and 680 (s.e. 34·0) g d^?1. Inoculant treatment increased DM (P < 0·01), organic matter (P < 0·01), crude fibre (P < 0·05), neutral detergent fibre (NDF) (P < 0·05) and energy (P < 0·05) digestibilities, the digestible organic matter concentration (P < 0·01) and the ME concentration (P < 0·05) of the total diets. Additive treatment altered rumen fermentation patterns but had little effect on the rumen degradability of silage DM, modified acid detergent (MAD) fibre, NDF or hemicellulose. It is concluded that treatment with the inoculant improved silage fermentation and increased digestibility, had little effect on silage DM intake but significantly increased carcass gain to a level similar to that sustained by a well-preserved formic acid-treated silage     

The effects of stage of growth and additives with or without cellulase on fermentation and in vitro degradation characteristics of Leymus chinensis silage

This study evaluated the effects of different combinations of added lactic acid bacteria and cellulase applied at two growth stages on chemical composition and in vitro rumen digestibility of Leymus chinensis silage. Fresh grass was harvested at early heading stage (S1) and late heading stage (S2), respectively, and ensiled with five additives: 200 U cellulase (C) kg^?1 fresh matter (FM), 1 × 10⁵ colony‐forming units (cfu) Lactobacillus plantarum (LP) g^?1 FM, 1 × 10⁵ cfu Lb. casei (LC) g^?1 FM, LP+C, LC+C and a control (CK). Four replicates of each treatment were weighed into 5‐L plastic buckets, and the mini silos were stored at ambient temperature (~30°C) for 60 d. Leymus chinensis harvested at S2 showed relatively higher neutral detergent fibre content, coliform bacteria count and lower crude protein content than S1. All additives decreased the pH and ammonia nitrogen (NH₃‐N) content of L. chinensis silage (P < 0·001) except C. LP+C and LC+C decreased fibre content and increased water‐soluble carbohydrate content (P < 0·001). The silages were further anaerobically incubated in vitro at 39°C for 48 h with buffered rumen fluids of lactating cows. Leymus chinensis harvested at S2 showed lower in vitro dry‐matter disappearance, NH₃‐N, total volatile fatty acid (VFA) content and higher average gas production rate (P < 0·05) than S1. In conclusion, Leymus chinensis should not be harvested too late. Compared with other treatments, a combination of Lb. casei (LC) with cellulase resulted in better fermented silage, but further testing is needed to confirm its efficacy.     

The effect of molasses‐based pre‐fermented juice on the fermentation quality of first‐cut lucerne silage

The aim of this study was to evaluate the possible effect of pre‐fermented juice (PFJ) on the fermentation quality and nutritive value of first‐cut lucerne (Medicago sativa L.) silage. The PFJs were prepared using barley (B), wheat (W) and grass herbages (G). Both fresh (PFJ‐B, PFJ‐W and PFJ‐G) and frozen (PFJ‐B_F, PFJ‐W_F and PFJ‐G_F) PFJs were examined. Frozen PFJs were prepared by freezing fresh PFJs at ?22°C with 20% glycerol (v/v). Treatments of lucerne silage included (1) control; (2) silage treated with PFJ‐B; (3) silage treated with PFJ‐W; (4) silage treated with PFJ‐G; (5) silage treated with PFJ‐B_F; (6) silage treated with PFJ‐W_F; and (7) silage treated with PFJ‐G_F. All the treatments consisted of five replicate silos, and they were prepared in 1·0‐L glass jar silos. Results showed that silages treated with fresh and frozen PFJs, regardless of plant material, had better fermentation quality than the control silage in terms of lower pH, butyric acid (BA) and ammonia nitrogen (NH₃‐N) concentrations, as well as higher lactic acid (LA) concentration (P < 0·05) and in vitro organic matter digestibility (IVOMD), metabolizable energy (ME) content, and gas production values (P < 0·05). Results indicated that PFJ treatments enhanced the nutritive value, fermentation quality and IVOMD, ME content and gas production values of first‐cut lucerne silages.     

A short-term comparison of the performance of four grassland-based systems of milk production for autumn-calving dairy cows

Eighty winter‐calving dairy cows of mixed parity were managed in four grassland‐based systems of milk production (F‐F, F‐C, C‐F and C‐C) over a full lactation (year 1) and during the winter period of the subsequent lactation (year 2). During the winter periods cows on systems F‐F and F‐C were offered silages of high feeding value, supplemented with 6·0 kg d^?1 of concentrate [crude protein (CP), 307 g kg^?1 dry matter (DM)] through an out‐of‐parlour feeding system, while cows on systems C‐F and C‐C were offered silages of medium feeding value, supplemented with c. 12·8 kg d^?1 of concentrate (CP, 204 g kg^?1 DM), in the form of a complete diet. After 25 February in year 1, cows on systems F‐F and C‐F were given access to grazing for periods of increasing duration, achieving full turnout on 17 April. Thereafter, until 21 October, these cows were offered a high daily allowance of herbage within a flexible grazing system (23·0 kg DM per cow, measured above a height of 4·0 cm), supplemented with 0·5 kg d^?1 of a ‘high magnesium’ concentrate. Cows on systems F‐C and C‐C (year 1) commenced grazing on 1 April, achieving full turnout on 17 April. Thereafter, until 20 October, these cows were managed on a restricted allowance of herbage in a rotational paddock grazing system, with concentrates (average allocation, 3·9 kg d^?1) being offered according to yield. In year 2, cows on systems F‐F and C‐F were given access to grazing for periods of increasing duration, from 11 March to 8 April, at which point the study was terminated. With systems F‐F, F‐C, C‐F and C‐C, mean feed inputs and milk outputs (per animal) during year 1 of the study were as follows: total concentrate DM intakes [881, 1272, 1729 and 2171 kg (s.e.m. 96·1)]; total silage DM intakes [1722, 1713, 1047 and 1154 kg (s.e.m. 70·7)], total grass DM intakes (3245, 2479, 3057 and 2481 kg) and total milk outputs [7541, 7527, 7459 and 7825 kg (s.e.m. 305·8)] respectively. Stocking rates associated with each of the four systems were 2·2, 2·5, 2·6 and 2·9 cows per hectare respectively. Performance of dairy cows on the systems during the winter of year 2 was similar to that recorded during year 1. The results of this study indicate that similar levels of milk output, DM intakes, tissue changes and plasma metabolite profiles can be achieved from grassland‐based systems involving very different combinations of grass silage, grazed grass and concentrate feeds.     

The effect of sugar beet pulp-based silage additives on effluent production, fermentation, in-silo losses, silage intake and animal performance

First-harvest direct-cut, double-chopped grass (190 and 164g DMkg^?1 in Experiments 1 and 2 resptectively) was ensiled without an additive or, in Experiment 1, with 30 kg t^?1 grass of an absorbent additive based on sugar beet pulp (Sweet ‘n’ Dry) or with 3·441 t^?1 grass of formic acid and, in Experiment 2, with 30, 50 and 70 kg t^?1 grass of Sweet ‘n’ Dry or with 50kg t^?1 grass of unmolassed sugar beet pulp. The preservation and nutritive value of the silage, in-silo losses (including silage effluent production), silage intake and animal performance of adult and growing cattle were examined. In Experiment 1 all three silages were well preserved, although the formic acid-treated silage displayed significantly lower pH, ammonia nitrogen (NH₃N) [g kg^?1 total nitrogen (TN)] and volatile fatty acids (VFAs) than the other two silages. In Experiment 2 absorbent-treated silages displayed significantly lower pH, buffer capacity (Bc), NH₃N (gkg^?1 TN), CP, modified acid detergent fibre (MADF) and VFAs than untreated silage. Treatment of grass with the absorbent additives at ensiling resulted in reduced effluent production. In Experiment 1 each kilogram of Sweet ‘n’ Dry retained approximately 11 effluent, and in Experiment 2 silages made with Sweet ‘n’ Dry applied at 70kgt^?1 and sugar beet pulp applied at 50 kg t^?1 produced similar volumes of effluent and each kilogram of absorbent retained 1·0 and 1·31 of effluent respectively. In Experiment 1 sixty beef cattle [mean initial live weight (LW) 460 kg] were grouped according to LW and allocated to treatment at random. For untreated silage (unsupplemented or with 1 or 2 kg supplement head^?1 day^?1), absorbent-treated silage (unsupplemented or with 1 or 2 kg supplement head^?1 day^?1) and formic acid-treated silage (1 kg supplement head^?1 day^?1) the daily silage DM intakes were 6·12, 6·21, 6·40, 7·65, 7·45, 7·11 and 7·85 (s.e. 0·280) kg respectively, the daily liveweight gains were 0·22, 0·56, 0·81, 0·59, 0·74, 0·81 and 0·75 (s.e. 0·071) kg respectively and daily carcass gains were 0·31, 0·47, 0·67, 0·47, 0·61, 0·70 and 0·57 (s.e. 0·043) kg respectively throughout a 75-day feeding period. In Experiment 2, fifty-six growing cattle (mean initial weight 312 kg) were grouped according to LW and allocated to treatment at random. For untreated silage (unsupplemented or with 1·5 kg Sweet ‘n’ Dry or 1·5 kg commercial concentrates head^?1 day^?1), silage treated with Sweet ‘n’ Dry at 30, 50 and 70 kg t^?1 grass and silage treated with 50kg sugar beet pulp t^?1 grass the daily silage DM intakes were 5·46, 5·28, 5·33, 6·21, 6·27, 6·60 and 6·62 (s.e. 0·154) kg respectively and daily liveweight gains were 0·39, 0·75, 0·81, 0·63, 0·76, 0·94 and 1·75 (s.e. 0·052) kg respectively throughout a 122-day feeding period. In this experiment 360g kg^?1 more absorbent was required when it was included at ensiling rather than offered as a supplement to untreated silage to achieve the same individual animal performance.     

Enzymes as silage additives 2. The effect of grass dry matter content on silage quality and performance in sheep

Eight grass silages were made from two contrasting varieties of perennial ryegrass, four silages each from a late-cut early-maturing (high dry matter, HDM) variety and from an early-cut, late-maturing (low dry matter, LDM) variety. The grass was ensiled without additive (untreated), or with formic acid, or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2) for a period of 130 d.
Formic acid-treated silage had lower levels of lactic acid at both levels of dry matter than the other silages. Enzyme treatment of grass prior to ensilage resulted in reduced levels of cellulose, acid-detergent fibre and neutral-detergent fibre in LDM silages and lower acid-detergent fibre and neutral-detergent fibre in the HDM silages compared with the corresponding untreated and formic acid-treated silages. Voluntary intakes (g DM d⁻¹) of untreated and enzyme-treated silages were significantly ( P <0·01) lower at both digestibilities compared with formic acid-treated silages (LDM: untreated, 982; formic, 1069; enzyme 1, 868; enzyme 2, 937; HDM: untreated, 931; formic, 1027; enzyme 1, 943; enzyme 2, 914). The organic matter, carbohydrate and nitrogen digestibility coefficients of LDM silages were significantly ( P <0·001) higher than those of HDM silages. There were no significant differences in any component digestibility related to silage additive.
Comparison of digestibility coefficients for constituents of the LDM silages fed to sheep or steers showed no differences between species.