内蒙古绒山羊毛囊形态发生规律

以胚胎龄40~110 d内蒙古二郎山绒山羊胚胎体侧部皮肤为材料,通过石蜡切片、HE染色,研究绒山羊胚胎毛囊形态发生规律。结果显示,内蒙古绒山羊胚胎皮肤初级毛囊形态发生经历毛基板前期、毛基板期、毛芽期、毛钉期和毛囊期5个时期,这5个时期又可根据其形态结构的差异划分为不同的亚期,内蒙古绒山羊胚胎皮肤初级毛囊形态发生与汗腺的发生相伴随;内蒙古绒山羊皮肤初级毛囊形态发生是分批进行的。在胚胎龄40~110 d内蒙古绒山羊胚胎体侧部皮肤未观察到次级毛囊的发生。     

内蒙古阿尔巴斯绒山羊毛囊结构特征分析

以内蒙古阿尔巴斯绒山羊为材料,通过石蜡包埋技术制作皮肤组织切片,利用Sacpic染色法分析内蒙古阿尔巴斯绒山羊3月、5月和10月的毛囊结构,得到了3个时期较为清晰和具有明显时期特征的形态学图片,为阐明绒山羊绒毛生长发育提供了组织学基础.     

内蒙古绒山羊次级毛囊组织形态周期性变化研究

【目的】研究成年绒山羊次级毛囊组织形态的周期性变化过程,为研究绒山羊绒毛生长的分子调控机理奠定组织学基础。【方法】制作内蒙古绒山羊1年周期内每个月的皮肤组织切片,染色后显微镜下观察照相。【结果】成年绒山羊次级毛囊形态在一年中呈周期性变化,在兴盛期、退行期和休止期的形态各不相同。从4月份开始,次级毛囊外根鞘细胞向下分裂延伸,开始了毛囊的重建;8～9月份毛囊完成重建,毛囊结构完整;10月份毛球细胞停止分裂,毛乳头萎缩,大量细胞程序化死亡,毛根上移,毛囊进入退行期;12月份毛囊根部上升到皮脂腺附近不再变化;翌年1～3月毛囊的形态基本没有变化。【结论】了解了成年绒山羊次级毛囊1年内形态的周期性变化过程,为绒山羊绒毛相关领域的研究提供一定的借鉴。     

内蒙古阿尔巴斯绒山羊角蛋白5在毛囊生长周期中的表达

为探明角蛋白5(keratin 5,K5)在毛囊生长周期中的作用机制,采用免疫组化方法检测角蛋白5(K5)在内蒙古绒山羊毛囊生长周期中的表达情况。结果表明,在毛囊生长初期(6月),K5低表达于毛囊内根鞘和外根鞘;在毛囊生长旺盛期(10月),K5在内外根鞘同时表达,并在外根鞘表达量较高;在退行期(1月),K5在外根鞘低表达;在休止期(3月),K5基本不表达。由于K5的表达与绒毛生长周期发育规律一致,推测K5对绒毛的周期性生长可能是通过对内根鞘和外根鞘发挥作用的。     

内蒙古白绒山羊皮肤毛囊结构及选种方法的研究

对内蒙古白绒山羊皮肤毛囊结构和羊绒等１２个性状进行测定研究，估测了它们的遗传力，表型相关，和遗传相关，并在此基础上制订了选择指数，预期选择效果。     

内蒙古白绒山羊皮肤毛囊结构及选种方法的研究

对内蒙古白绒山羊皮肤毛囊结构和羊绒等12个性状进行测定研究,估测了它们的遗传力,表型相关,和遗传相关,并在此基础上制订了选择指数,预期选择效果。     

中国绒山羊的毛囊结构特性与发育机制

绒山羊业是中国畜牧业的重要组成部分,其皮肤次级毛囊所产的山羊绒是毛纺工业中最优质的天然纤维原料,也是中国国际贸易份额比重最大的畜产品.绒山羊皮肤次级毛囊是山羊绒纤维的“发源地”,因而绒山羊皮肤毛囊的结构特点、遗传特性、发生发育与周期性变化及调控因素,是了解绒山羊种质特性的基础,也是绒山羊选育研究的基础.笔者在查阅国内外对绒山羊皮肤毛囊研究文献的基础上,结合多年的研究结果,综述了中国绒山羊毛囊性状与发育调控研究的现状与进展,以期为指导绒山羊育种提供理论基础.     

毛囊形态发生变化过程分子调控研究进展

在介绍毛囊结构的基础上,阐述了毛囊形态发生变化过程及其相关调控分子作用机制方面的研究进展。     

pcdh19在小鼠毛囊形态发生过程中的时空表达

以胚龄13.5 d到8日龄的昆白系小鼠为试验材料,应用免疫组化方法测定pcdh19在小鼠毛囊形态发生中表达的时空变化。结果显示,基板前期、基板期均未发现pcdh19的表达;毛芽期开始表皮和间充质胞质中出现弱表达,并且毛芽上的表达逐渐加强;在毛囊结缔组织鞘发生和成熟过程中,在该部位始终强表达,推测可将pcdh19作为结缔组织鞘的标志分子。     

内蒙古绒山羊Hoxa7基因在毛囊中的表达

利用原位杂交技术检测Hoxa7基因在毛囊不同生长时期的表达模式,结果表明,Hoxa7基因分别在胚胎期的95 d初级毛囊的内根鞘表达、在115 d次级毛囊的内根鞘和绒干表达、在125 d初级毛囊内根鞘和毛干皮质表达、在兴盛期10月的初级毛囊毛干皮质和次级毛囊的绒干中表达。揭示在毛囊发育中,Hoxa7基因对于毛囊细胞的增殖可能起一定的作用。     

Hoxc13 Expression Pattern in Cashmere Goat Skin During Hair Follicle Development

Hoxc13 has an important role in controlling hair formation. In this study, we examine the Hoxc13 RNA expression pattern of skin during embryo development. The result indicated that changes of the Hoxe13 gene expression and thickness of skin have a similar trend during hair follicle morphogenesis. In interpreting these results, we investigated whether the regulation motifs is in Hoxc13 intron, which is a 5.4 kb fragment. To blast with other mammals, we found a very conservative region in all mammal animals and two regions in livestock, such as cow, sheep, horse, dog, and so on, which are not in other Hox genes. We have examined putative pre-miRNA in this region, providing an entry point for elucidating currently unknown mechanisms that are required for regulating quantitative levels of Hoxc13 gene expression.     

Hoxc13/β-catenin Correlation with Hair Follicle Activity in Cashmere Goat

Seasonal hair follicle activity and fibre growth in some Cashmere-bearing goats (Caprus hircus) is a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle activity and cycling, we used qPCR to investigate 19 genes expression in Cashmere goat side skin from 12 mon. Many of these genes may be associated with the hair follicle development-relevant genes (HFDRGs) in the literature. Here we show that Hoxc13/β-catenin gene associated with the follicle activity. In addition, Hoxc13 was found to be expressed with an drastic increase between July and November for melatonin treatments. To further investigate the role of Hoxc13 on HFDRGs, fibroblasts and keratinocytes from Cashmere goat skin were transfected with p-ECFPHoxc13. The result suggested that overexpression of Hoxc13 gene decreased HFDRGs with negative role for hair follicle development and increase HFDRGs with positive role for hair follicle development in vitro. These findings provide data on the Hoxc13 expression profile of normal Cashmere goat skin and Cashmere goat skin with melatonin treatment, and demonstrate hair-follicle-activity dependent regulation of Hoxc13 expression.     

内蒙古绒山羊产绒量和体重性状RAPD标记的初步研究

利用12条随即引物对27只内蒙古绒山羊基因组DNA进行预扩增,筛选出具有多态性扩增产物的4条引物进行RAPD分析,共得到51个标记,其中可变标记43个,标记条带多态性频率为0.84,扩增片断长度在176~2940bp之间。RAPD标记条带与经济性状关系分析表明:CY0816引物扩增产物的ABCFHO组合、F09引物的INR组合为内蒙古绒山羊产绒量的优势组合型;CY0816引物扩增产物的GIJOPQR条带组合为体重性状标记的优势组合型。     

雌二醇对体外培养绒山羊初级毛囊的影响(英文)

[Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in serum-free Williams E media supplemented with different doses of 17 β-E2 (0, 0.1, 1.0, 10.0, 100.0 nmol/L), and their growth rates and morphological changes were observed. [Result] The growth rate of 0.1 nmol/L 17 β-E2 group was quite comparable with that of the control group(0 nmol/L), but the 17 β-E2 with concentrations of 1.0, 10.0 and 100.0 nmol/L displayed different degrees of inhibition on the growth of hair follicles. Different morphological changes of hair follicles could also be discovered in different concentration treatments. [Conclusion] The study laid a certain foundation for exploring the regulation mechanism of estrogen on growth of cashmere goat hair follicles.