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Cees Waalwijk Pieter Kastelein Ineke de Vries Zoltan Kerényi Theo van der Lee Thamara Hesselink Jürgen Köhl Gert Kema 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(7):743-754
The re-emergence of fusarium head blight throughout the world and especially in Western Europe prompted a survey of the situation in the Netherlands. To allow for a high throughput screening of large numbers of samples, a diagnostic PCR method was developed to detect the most common species of Fusarium occurring on wheat. Seven primer pairs were tested for their ability to identify isolates of Fusarium avenaceum, F. culmorum, F. graminearum, F. poae, F. proliferatum and Microdochium nivale var. majus and M. nivale var. nivale. Each primer pair only generated a PCR product with the corresponding Fusarium species and all PCR fragments had different molecular sizes. This allowed the generation of these amplicons using a mixture of all seven primer pairs. The robustness of this multiplex PCR encouraged us to screen a large series of isolates collected in 2000 and 2001. In both years 40 fields were sampled leading to a collection of 209 isolates from 2000 and 145 isolates from 2001. The results of the multiplex PCR demonstrated that F. graminearum was the most abundant species in the Fusarium complex on wheat in both years. This is in sharp contrast to reports from the 1980s and early 1990s, which found F. culmorum as the predominant species. Primers derived from the tri7 and tri13 genes, which are implicated in the acetylation and oxygenation of the C-4 atom of the backbone of the trichothecene molecule, were used to discriminate between deoxynivalenol and nivalenol (NIV) producers. The populations of F. culmorum and F. graminearum both showed a slight increase in NIV-producers in 2001. 相似文献
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新疆奇台县近42a气候变化特征分析 总被引:5,自引:1,他引:4
根据新疆奇台县气象站的气温及降水资料,利用线性趋势函数及M ann-kendall突变检测法分析了该地区近42年的气候变化。结果表明:年平均气温与极端最低气温均呈上升趋势,而极端最高气温呈下降趋势;年降水量呈上升趋势。从季节变化来看,除夏季平均气温呈下降趋势,其它各季平均气温均呈上升趋势,其中以冬季增幅最大,秋季次之;各季降水均呈上升趋势,其中以夏季增幅最大,冬季次之。年平均气温未发生突变,而年降水量在1984年发生由少向多的突变。对气温和降水的异常分析得出,暖温年多发生在上世纪80年代后,冷温年多发生在上世纪60、70年代;多雨年主要在近20年,少雨年则多在上世纪60、70年代。此外,该县近42 a来增暖幅度小于全疆和北疆;增湿幅度大于全疆,小于北疆。 相似文献
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弹状病毒含有单链负义RNA基因组,寄主范围比较广泛,能侵染无脊椎动物、脊椎动物以及植物等寄主,对人类的健康、农作物产量和自然生态系统造成严重威胁。植物弹状病毒主要根据复制场所分为细胞质弹状病毒属Cytorhabdovirus和细胞核弹状病毒属Nucleorhabdovirus,其基因组由单股负链RNA组成。此外,Dichorhavirus和Varicosavirus病毒属是两个新鉴定的植物弹状病毒属,其特征是具有二分体基因组。本文着重介绍危害我国农作物的几类植物弹状病毒,对它们的病理学、病害流行、基因组信息以及传播媒介进行概述,为中国植物弹状病毒病害的研究提供理论依据。 相似文献
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组蛋白修饰作为表观遗传修饰的一种主要形式,对基因表达和表型调控具有重要作用。组蛋白修饰的N端尾区可通过乙酰化、甲基化、磷酸化等修饰来改变染色质的状态以及调控基因的表达。与脊椎动物相比,昆虫种类繁多,且有变态发育、表型复杂等特征,可以成为探索动物社会行为、发育调控和毒理作用等表观遗传基础的模型。本文总结了昆虫组蛋白修饰的主要类型(乙酰化和甲基化修饰)及修饰酶的研究进展,对染色质免疫共沉淀测序技术(chromatin immunoprecipitation followed by sequencing,ChIP-seq)、染色质转座酶可及性测序技术(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)、转录组测序技术(RNA-seq)、组蛋白修饰酶功能验证以及Western blot、免疫细胞化学(immunocytochemistry,ICC)、免疫组织化学(immunohistochemistry,IHC)、酶联免疫吸附测定(enzyme linked immunosor... 相似文献
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综述了我国苜蓿主要病害的分布和危害、病原学、发生规律、抗性种质材料的遗传筛选和鉴定及综合防治等方面的主要研究进展,并提出了今后的研究方向和发展目标。 相似文献
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采用Odum能流分析理论以及主成分分析法,结合磴口县1992~2008年统计年鉴,选取有机能、无机能、能量总投入和总产出、产投比、能量循环指数和投能结构6个指标,对磴口县农林复合生态系统农田子系统的能流特征进行定性、定量分析.结果表明:该地区有机肥使用量逐年降低,而无机肥却在增加;该系统能量产出大于投入,产投比为:3.... 相似文献
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Variation in pesticide residues in space and time has not been investigated systematically in spite of the large variability found at single scales (e.g. between trees or orchards). Information on variability at different scales will support the development of sampling methods and more reliable prediction of residues. Experiments were conducted to quantify the spatiotemporal variability in initial spray deposit on apple leaves and fruit using a substitute tracer (zinc EDTA chelate). Five hierarchical spatial scales were defined as (1) between orchards, (2) between plots within an orchard, (3) between trees within a plot, (4) between zones within a tree and (5) between leaves/fruit within a zone. Similarly, two temporal scales were defined as (1) between applications within a single year and (2) between years. The initial zinc concentration was approximately log-normally distributed; the variability in initial deposit concentration was greater on leaves than on fruit. The average initial zinc deposition was significantly greater in the top and outside zones than in the middle zones within individual trees. The most important scale for driving residue variability is the within-zone unit-to-unit variation, contributing to 71 and 49% of the observed variability for leaf and fruit samples respectively. Variability at other scales was related to the variation in the tree architecture. The results indicated that stratified sampling on the basis of within-tree zone structures, with the effort focused on within-zone sampling, should be used for the determination of pesticide residues. 相似文献
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西安地区丰水年农田深层土壤含水量研究 总被引:1,自引:0,他引:1
通过对西安南郊吴家坟、长安区郭杜镇大学城和双竹村附近丰水年(年降雨量883 mm)麦地、玉米地土壤含水量的测定,以及与正常年份该地土壤含水量的对比,研究丰水年该地区农田0~6 m土壤含水量的变化。结果显示,正常年份西安地区麦地0~2 m土层含水量高于12%,2~6 m土层含水量高于13%;丰水年西安地区麦地0~2 m的土壤含水量在18%以上,2~6 m土层含水量达24%以上,远远高于正常年份这一层位的土壤含水量。分析得出,西安地区农田深层土壤含水量高的主要原因是重力水带分布范围大于或等于农作物的强烈耗水层范围。 相似文献
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河北省小麦田杂草防治技术进展 总被引:3,自引:0,他引:3
小麦是河北省第一大种植作物 ,常年种植面积2 4 6 .7万hm2 左右。随着农民种植水平、农业机械化程度的不断提高 ,麦田杂草成为制约小麦高产的主要障碍之一。全省小麦田杂草的发生面积超过2 13 3万hm2 ,每年因草害造成小麦产量损失 10 %~15 % ,重发田块高达 30 %~ 5 0 %。为了提高杂草的防除水平 ,河北省植物保护总站从 1998年在农业厅立项实施了小麦田杂草无害化治理技术与推广 ,几年来在广大植保技术人员的共同努力下 ,经过大量试验、示范以及组织推广 ,使麦田杂草的除治工作有了较大进步。1 基本摸清了麦田常见杂草种类和群落分布1.1 … 相似文献
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V. G. IVANYUK 《EPPO Bulletin》1998,28(4):475-479
The control of potato diseases in Ukraine is reviewed. Control of potato blight (Phytophthora infestans) has only been adequate for limited periods in the past (when resistant cultivars were available and when the systemic fungicides were introduced). Currently, the disease is in a new epiphytotic phase and control has failed again, probably connected with the introduction of new mating types and the existence of greater variability. Blight now occurs earlier in the season and in most years (instead of only years when climatic conditions are favourable). Control has now to be essentially preventive, rather than based on forecasting. The importance and control of other potato diseases is also considered (Alternaria spp., Rhizoctonia solani, Geotrichum candidum, Phytophthora erythroseptica, Sclerotinia sclerotiorum, Streptomyces scabies). 相似文献
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Self-incompatibility in Mikania micrantha in South China 总被引:1,自引:0,他引:1
Mikania micrantha is a successful invasive weed in many parts of the world, but we do not know how its mating system may have contributed to this. Therefore, its mating system was studied in an open M. micrantha population during the flowering seasons of 2004 and 2005. Pollen/ovule ratio was determined and pollination treatments were applied on entire inflorescences to estimate the seed/ovule ratio. The pollen/ovule ratio of M. micrantha was 4008, and seed/ovule ratio was 0.56 for open pollination and 0.0034 and 0.0038 for wind pollination and selfing respectively. These data indicate that the weed is self‐incompatible and needs insect pollination. 相似文献
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Because of their special behaviour, physiology and close relationship with humans, mosquitoes act as one of the most important vectors of human diseases, such as filariasis, Japanese encephalitis, dengue and malaria. The major vector mosquitoes are members of the Culex, Aedes and Anopheles genera. Insecticides play important roles in agricultural production and public health, especially in a country with a huge human population, like China. Large quantities of four classes of insecticides, organochlorines, organophosphates, carbamates and pyrethroids, are applied annually to fields or indoors in China, directly or indirectly bringing heavy selection pressure on vector populations. The seven major species of vector mosquito in China are the Culex pipiens L. complex, C. tritaeniorhynchus Giles, Anopheles sinensis Wied., A. minimus Theobald, A. anthropophagus Xu & Feng, Aedes albopictus (Skuse) and Ae. aegypti L., and all have evolved resistance to all the above types of insecticide except the carbamates. The degree of resistance varies among mosquito species, insecticide classes and regions. This review summarizes the resistance status of these important vector mosquitoes, according to data reported since the 1990s, in order to improve resistance management and epidemic disease control, and to communicate this information from China to the wider community. 相似文献
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N. ADIGÜZEL 《EPPO Bulletin》1981,11(2):19-22
Sunn pest ( Eurygaster integriceps ) is the most serious and widespread pest of cereals in south-eastern Turkey. It is very important to estimate its spring populations, and the area possibly needing to be treated, a few months before they migrate to the fields. The field populations of the pest and the severity of epidemics can be forecast from the population density of overwintering adults per plant in the overwintering localities. To do this, surveys have been made since 1955 on 25 selected mountain sites. Parallel to these investigations, the population of overwintered adults per m2 in cereal fields has been determined in order to fix the area to be treated, since 1956. A relation can be seen between the population density of overwintered adults per plant and the next season's populations in the infested areas. 相似文献
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Viruses occurring in Plantago species in England 总被引:1,自引:0,他引:1
JOHN HAMMOND 《Plant pathology》1981,30(4):237-243
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山仑 《干旱地区农业研究》2023,(3):2-4
介绍了我国旱地农业发展的历程、成就和经验,并重点就农业结构调整和农田持续增产的技术途径进行了论述,认为培育抗旱节水新品种和发展半旱地农业是农田稳定持续增产的突破口,而通过调整种植业结构以发展草地畜牧业则是半干旱地区实现生态环境改善和生产力同步提升的关键。此论述以期对指导半干旱地区农业发展和生态环境改善产生积极意义。 相似文献