ELISA双抗体夹心法诊断兰州地区幼犬轮状病毒病

应用ELISA双抗体夹心法对兰州地区幼犬轮状病毒感染情况进行调查,结果表明该地区幼犬轮状病毒感染阳性率为57.1%,其中2～4月龄的幼犬占阳性病例的75.0%,4月龄以上者感染率最低为42.86%.     

犬呼吸道感染病例调查与分析

为了调查当前徐州地区犬呼吸道感染情况,试验采用翻阅门诊病历的方法,对2014年间呼吸道感染病例进行了分析,结果表明犬呼吸道感染占门诊犬病的比例为8.6%,4~6月份多发,1岁以内幼犬易感,2月龄幼犬最易感,占32.1%,发病时体温变化不明显。     

犬细小病毒病的诊治

<正>犬细小病毒病是由犬细小病毒(CPV)引起的一种急性热性传染病。该病在临床上表现各异,但是主要表现为出血性肠炎和非化脓性心肌炎两种病型,个别肠炎型病例伴有心肌炎变化。该病多发于未注射疫苗的3~6月龄幼犬。1流行病学CPV主要感染犬,传染性极强,死亡率也高,不同年龄、性别、品种的犬均可感染。尤其6月龄以下幼犬最易感,占CPV感染犬总数的75%,而且此日龄段感染犬的死亡率大于6月龄患犬。该病的发生没有明显     

中西结合治疗幼犬犬细小病毒的报告

犬细小病毒病是由犬细小病毒(Canine parvovirus,CPV)感染引起的,以严重肠炎综合征和心肌炎综合征为特征的对犬危害极其严重的烈性传染病.幼犬发病率和死亡率都很高,各种年龄、性别和品种的犬均易感,但纯种犬和2～4月龄幼犬易感性较高,发病率和死亡率高达45%～100%.     

犬瘟热的诊断与防治概要

犬瘟热是一种急性传染病,肉食动物多发,尤其是幼犬最容易发病。笔者通过对28例犬瘟热的临床观察总结了一套防治方案,取得了较好效果,现介绍如下。1临床症状笔者接诊的28例幼犬中2～3月龄的16例,3～4月龄的5例,4月龄以上的7例。所有病例均有发热、咳嗽、呼吸急促表现,体温多在40～41℃     

中西医结合治疗幼犬细小病毒病的报告

犬细小病毒病是由犬细小病毒(Canine parvovirus,CPV)感染引起,以严重肠炎综合征和心肌炎综合征为特征的对犬危害极其严重的烈性传染病。该病幼犬发病率和死亡率都很高,各种年龄、性别和品种的犬均易感,但纯种犬和2~4月龄幼犬易感性较高,发病率和死亡率高达45%～     

猪轮状病毒油乳剂灭活疫苗的制备及免疫原性试验

猪轮状病毒(porcine rotavirus)属呼肠孤病毒科(Reoviridae)、轮状病毒属(Rotavirus),是引起仔猪发生急性胃肠道传染病的重要病原,自1975年Woode和Bridge首次从猪体分离出猪轮状病毒以后,世界各国均有不同程度的猪轮状病毒感染报道.在我国猪轮状病毒感染亦十分普遍,李国平等在调查中发现仔猪1～10日龄阳性率42.4%～66%,10日龄到断奶阳性率83.2%～91.7%,断奶后阳性率为63.2%～72%[1].临床症状主要表现为恶心、呕吐、腹泻,成年猪一般呈隐性感染.鉴于猪轮状病毒感染普遍,危害大,对猪进行疫苗接种预防猪轮状病毒感染具有重要意义.     

犬冠状病毒与细小病毒混合感染的诊断与治疗

<正>近年来在笔者收治的病犬中,以混合感染的病例较多,本病多发于3~6个月龄的幼犬,以秋、冬季节多发,主要表现为胃肠炎,少数心肌炎型(多发生于1~3月龄的幼犬)。     

犬细小病毒病的诊断与对症治疗

<正>犬细小病毒病,是由犬细小病毒(CPV)引起的犬科动物的一种具有高度接触性传染的烈性传染病,临床上以急性出血性肠炎和心肌炎为特征。感染率高,并且死亡率很高。该病一年四季均可发生,一般夏秋季多发,不同年龄、性别、品种的犬均可感染,并且死亡率很高,有时其感染率可高达100%,致死率为10%～50%。一月龄以下的幼犬多呈急性致死性心肌炎,2～6月龄犬多呈出血性肠炎病变。1病例资料靖远县乌兰镇一雄性4月龄犬,体重2.2kg。     

幼犬轮状病毒性腹泻的诊治

<正>犬轮状病毒性腹泻主要发生在幼犬,成年犬一般不表现症状或症状轻微。轮状病毒主要在肠道内增殖,并随粪便排出而污染环境。一般情况下,发病犬有腹泻症状时,最先需要诊断的有细小病毒病、犬瘟热、冠状病毒病、球虫这4种疾病,如果排除这几种病,一般会认为是消化不良或细菌性感染,因此犬轮状病毒性腹泻容易被忽视。笔者于2013年11—12月份期间收治了几例犬轮状病毒感染病例,现将症状、诊断及治疗做一叙述,希望能引起宠物医生的     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.