不同浓度氟化物对鸡红细胞微核形成的影响

为研究氟化物对鸡红细胞微核形成的影响,选用36只1日龄罗曼褐公鸡,随机分为6组,每组6只,第六组为对照组,第一到第五组为实验组,在饮水中添加不同水平的氟化物,使水中氟的含量分别为2.5、1.25、0.625、0.3125、0.1563mg/mL,试验期为3个月。生物统计结果表明,0.1563mg/mL剂量组,红细胞微核细胞率与对照组无明显差异,其他各组微核细胞率随氟剂量的增高而升高(P<0.01)。结果表明氟化物为潜在的诱变物。     

镉对鲫血红蛋白的影响

研究了镉对鲫血红蛋白的影响。急性染镉导致鲫Hb在短时间内的急剧变化与红细胞数量的急剧变化有关；摄入高剂量镉的鲫在第30天时出现缺铁性贫血，摄入低剂量镉组未使鲫出现缺铁性贫血。     

中药复方Ⅱ对鲤鱼耐缺氧能力、RBC及HB的影响

研究了中药复方Ⅱ对鲤鱼耐缺氧能力和红细胞(RBC)数及血红蛋白(HB)含量的影响。选用体格健康的鲤鱼80尾,平均分成4组,即基础饲料组(对照组)和3个基础饲料加中药组(实验组),后3个实验组设了3个中药添加量。用上述4种饲料对实验鱼饲养120 d后,测定鲤鱼密闭性缺氧浮头时间、红细胞数和血红蛋白含量。结果显示:3个剂量的中药复方Ⅱ实验组能不同程度地延长缺氧条件下鲤鱼浮头时间,增加鲤鱼血液中红细胞数和血红蛋白含量,但低剂量组与对照组相比,差异不显著(P>0.05)。本研究表明中药复方Ⅱ能提高鲤鱼耐缺氧能力,并有改善外周血象的作用。     

饥饿和水霉病对异育银鲫血液指标影响的研究

研究了饥饿和水霉病对异育银鲫血液指标的影响。饥饿前期(前6 d),饥饿对异育银鲫血液的影响主要体现在血红蛋白和红细胞合成数量的降低;饥饿中期(第7~12天),饥饿不仅影响异育银鲫血红蛋白的含量和红细胞的合成,而且此时血液中红细胞体积变小,畸形率增加;饥饿后期(第15~24天),红细胞的血红蛋白继续降低,但是红细胞的数量和比容维持在一个稳定的水平。饥饿对异育银鲫红细胞的渗透脆性影响不大。患水霉病的异育银鲫血红蛋白、红细胞数量和红细胞比容均比健康鱼低,水霉病主要降低红细胞的合成数量和减小红细胞的体积。     

EM对大口鲶血液学指标影响的研究

用微生态制剂EM作为饲料添加荆饲养大口鲶，经40天的试验，测定其血液学指标(包括红细胞数、白细胞数、血红蛋白含量、红细胞大小、红细胞渗透脆性、白细胞分类记数、红细胞沉降率和红细胞比积)。结果显示：试验组大口鲶的红细胞数和血红蛋白含量等指标均优于对照组。表明EM能改善大口鲶的血液学指标，提高机体的新陈代谢，加快生长速度。     

饲料Vc对异育银鲫的生理效应及其适宜添加量

在精制饲料中分别添加不同剂量Vc(0、37.5、75、15 0、30 0、6 0 0和 12 0 0mg/kg) ,连续投喂异育银鲫 (方正银鲫♀×兴国红鲤♂ ) (Carassiusauratusgitelio) 8周。结果显示 ,当饲料中缺乏Vc时异育银鲫虽没有出现明显的缺乏症状 ,但日增重率、血红蛋白含量和血清POD酶活性均低于Vc添加组 ,而红细胞脆性极显著地高于高剂量Vc添加组 (P <0 .0 1) ;同时 ,经新复极差检验 ,在适宜范围内随着饲料Vc含量增加 ,异育银鲫日增重率、血红蛋白含量、POD活性等指标显著提高 ,而红细胞脆性明显下降 (P <0 .0 5 )。综合以上指标的非线性回归分析结果 ,建议生产上异育银鲫饲料Vc适宜添加量为 30 0～ 5 0 0mg/kg。     

浅谈奶牛焦虫病的综合性治疗

<正>奶牛焦虫病是由巴贝斯虫或泰勒斯虫寄生于牛红细胞内的一种血液原虫病,临床上以高热、贫血、黄疸、血红蛋白尿、迅速消瘦和产奶量降低为特征,对奶牛生产危害极大。     

螺旋藻对异育银鲫血液生理指标和非特异性免疫的影响

选用均重3.2 g异育银鲫夏花483尾,随机分为7组,每组设3个重复.对照组饲喂基础日粮,试验组在基础日粮中分别添加0.2%、0.5%、1.0%、1.5%、2.0%和5.0%的螺旋藻.试验结果表明:①试验组的溶菌酶活性均显著高于对照组(P＜0.05),分别比对照组提高了36.49%、97.58%、104.00%、102.00%、118.00%、92.64%;②试验组吞噬指数均大于对照组,其中添加1.0%、2.0%、5.0%螺旋藻组与对照组相比分别提高了59.13%、84.35%、123.00%,且差异显著(P＜0.05);③试验组的血红蛋白与对照组相比差异不显著;④试验组红细胞数量与对照组差异不显著,白细胞数量有增加的趋势.     

关于新生仔猪溶血症防治探讨

<正>新生仔猪溶血症(Hemolytic disease in newborn piglets)是指新生仔猪吃初乳而引起红细胞溶解的一种急性、血管内溶血性疾病。仔猪以贫血、黄疸和血红蛋白尿为主要特征,致死率可达100%。该病的实质是由于母畜血清抗体与新生仔畜红细胞抗原不合,引起的一种同种免疫溶血性反应的病理过程。是母猪血清和初乳中存在抗仔猪红细胞抗原的特异血型抗体所致的。     

油脂对肉鸭生产性能的影响

本次试验选用200只1日龄樱桃谷商品肉鸭,随机分成4组,每组50只,试验I组、II组和III组在前期日粮中分别添喂3%、6％和9%的大豆油;对照组只喂基础日粮,不喂大豆油,结果表明：各试验组42日龄平均体重与对照组相比较差异显著（P＜005）,分别提高体重33g、389、52g,以添加9％的比例效果最好：饲料中添加大豆油对肉鸭成活率无任何不良影响,且能提高饲料的转化率     

患肌肉溃烂病中华倒刺鲃血细胞数量及形态变化

采集嘉陵江中网箱养殖的中华倒刺鲃(Spinibarbus sinensis Bleeker)患肌肉溃烂病病鱼和健康鱼,对比测定二者红细胞数量、白细胞数量、血红蛋白含量、红细胞大小等指标,以研究病鱼血细胞的形态和数量变化。结果表明病鱼和健康鱼鱼体的多项指标存在显著差异。相对于健康鱼,病鱼外周血中红细胞数目显著减少,白细胞数目极显著增加,红细胞有变小趋势,表明肌肉溃烂对中华倒刺鲃红细胞有一定破坏作用;相对于健康鱼,病鱼淋巴细胞比例下降,单核细胞比例升高,表明在病理情况下,鱼体的特异性免疫功能降低,吞噬功能和非特异性免疫功能有所增强。     

嗜水气单胞菌对金鲫血液学指标的影响

用嗜水气单胞菌对金鲫进行攻毒试验,对试验鱼进行血液有形成分指标和血液生化指标的测定.结果表明:单核细胞数、谷丙转氨酶(GPT)、总胆固醇(CHO)、钾(K)对照组与试验组差异显著(P<0.05);红细胞总数、淋巴细胞、嗜中性粒细胞、谷草转氨酶(AST/GOT)和胆碱酯酶(CHE)试验组与对照组差异极显著(P<0.01)...     

饥饿胁迫对杂交鳢血液指标的影响

探索杂交鳢在饥饿不同时间状态下5项血液指标的变化情况。测定并比较饥饿处理7、14、21、28、35和42d实验组和对照组杂交鳢血液指标。结果显示,经过42d的饥饿处理,对照组与6个实验组相比,随着饥饿时间的延长,红细胞数量在0~42d内呈现下降的趋势,但在28d时测得红细胞数量突然增多,几乎达到了对照组水平,为(2.29±0.46)×1012/L。血红蛋白0~14d内呈现升高的趋势,第14d达到最高,为(82.36±7.26)g/L,在14~42d内整体上呈现降低的趋势,但在28d时测得血红蛋白含量突然升高,与14d测得的结果相当。沉降率在0~28d呈下降的趋势,28~42d内又明显上升。渗透脆性呈下降趋势。白细胞数量在0~42d内一直增加。     

日粮铁对草鱼生长、营养成分和部分血液指标的影响

选用(7.12±0.22)g的草鱼(Cenopharyngodon idellus)鱼种,分别在纯化日粮中添加铁0,100,200,300,400,500 mg/kg,进行为期78 d的生长试验以考察铁对草鱼生长性能、营养成分和部分血液指标的影响。试验结果显示,日粮中添加铁对草鱼的生长、体成分和血红蛋白和红血细胞数目等都有显著影响。日粮中添加铁300 mg/kg时,草鱼增重率、特定生长率、饲料效率、蛋白质效率比、蛋白沉积效率达最大值;体蛋白和肌肉蛋白含量高,脂肪含量低,灰分含量高;血红蛋白和红血细胞均达最高水平。结果说明:草鱼纯化日粮中铁的适宜添加量为300 mg/kg。     

Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.)

Two experiments were conducted to evaluate the efficacy of clove solution on anesthesia and hematological parameters in Beluga, Huso huso (L.). In the experiment 1, fish were exposed to concentrations of 300, 500, 700, 1,800 and 3,000 ppm clove solution and induction (stage 3 and 4) as well as recovery period lengths were recorded. These concentrations were led to induction and recovery time between 42–365 and 125–235 s, respectively, in dose-dependent manner. There was negative correlation between induction and recovery time (R ² = 0.92). In the experiment 2, fish were exposed to each aforementioned concentrations and let to reach stage 4 anesthesia and then blood-sampled. Control group was sampled without anesthesia. Hematological studies between groups showed no significant changes in white blood cell (WBC) and differential leukocyte count (P > 0.05). Hematocrit (Hct), hemoglobin (Hb), red blood cell (RBC) and mean corpuscular hemoglobin concentration levels were significantly high in concentrations of 300–700 ppm compared to control, 1,800 and 3,000 ppm. Control and 500–3,000 ppm groups have similar mean corpuscular volume values and significantly higher than 300 ppm. Lower values of mean corpuscular hemoglobin were related to control, 3,000 and 1,800 ppm and higher values were observed in the others. Strong positive correlation were observed between induction periods and Hct, Hb and RBC levels (R ² = 0.095, 0.99 and 0.92, respectively). Results suggested that anesthesia with low concentrations of clove solution over a long period is stressful compared to high concentrations over short period.     

Protective effect of dietary curcumin in Anabas testudineus (Bloch) with a special note on DNA fragmentation assay on hepatocytes and micronucleus assay on erythrocytes in vivo

The present study was conducted to evaluate the safety of long-term dietary curcumin at doses 0.5 and 1 % in Anabas testudineus employing hematological and cytological techniques. The fish were fed with curcumin-supplemented feed for 6 months. Fine blood smears were prepared and subjected to three different staining techniques. The erythrocyte micronucleus frequency (MN) and the cytometric measurements of erythrocytes were determined. Blood from the control and treated fish was subjected to the assessment of several hematological parameters. Also, DNA fragmentation assay on hepatocytes was conducted. The results showed that hemoglobin content, RBC count and hematocrit increased in the curcumin-fed fish compared to control, whereas WBC count, platelet count, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were unaffected. WBC/RBC ratio was lower in the case of curcumin-treated fish. The cytometric measurements revealed no change in the erythrocytes and their nuclei after curcumin treatment. DNA fragmentation assay revealed intact DNA in curcumin-fed group, ruling out the possibility of curcumin-induced DNA damage. The positive control group showed a significant increase in MN frequency compared to negative control and curcumin-fed groups. In fact, the MN frequency decreased in 1 % curcumin-fed group compared to the negative control and 0.5 % curcumin groups. All these indicated a state of well-being of the curcumin-treated fish. Therefore, it is concluded that curcumin could be used as a safe feed ingredient to improve the growth of finfish in aquaculture.     

微生态制剂噬菌28对金鲫鱼血液指标的影响

利用不同剂量的微生态制剂(噬菌28)添加在饲料中饲养金鲫鱼8d、16d、24d,测定其与鱼体生长和健康相关的血液指标。结果显示:试验组红细胞压积、红细胞数、血红蛋白含量等血液指标相对于对照组均有明显变化,且随着微生态制剂添加量的增加和饲喂时间的延长而提高,表明噬菌28能改善金鲫鱼的血液指标,提高新陈代谢,促进鱼类健康生长。     

慢性氟中毒对鸡病理变化作用研究

为了探讨氟对鸡的病理变化作用研究,选用1月龄蛋公鸡36只,第Ⅰ组为正常对照组,第Ⅱ～Ⅵ组分别饮水添加氟化钠1.75g/L(1/27LD50),0.875g/L,0.438g/L,0.219g/L,0.109g/L,试验周期为90d,每组6只。结果表明,慢性氟中毒对鸡内脏器官的组织结构有明显损伤。     

Effects of dietary administration of Echinacea purpurea on growth indices and biochemical and hematological indices in rainbow trout (Oncorhynchus mykiss) fingerlings

This study was conducted to evaluate the effects of Echinacea purpurea (EP) on growth and some hematological and blood biochemical indices of rainbow trout fingerlings. A basal diet was supplemented with 0 (control), 0.25, 0.5, 1, and 2 g EP kg?1 to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish with an initial average weight of approximately 8 g. After 8 weeks of feeding trial, fish fed diets with 0.25 and 0.5 g EP kg?1 showed highest final weight and SGR and fish fed with the control diet indicated the lowest final weight and SGR. There was a significant (P < 0.05) decrease in FCR between the control group and the groups fed with diets of 0.25 and 0.5 g EP kg?1. Biochemical parameters such as serum total protein content, albumin content, globulin content, and albumin/globulin ratio in the fish were evaluated. There were significant differences between hematological parameters including RBC, WBC, HB, lymphocyte, and neutrophil percentage in fish fed with dietary nucleotide compared with control treatment (P < 0.05). The results suggest that EP administration at 0.25 and 0.5 g EP kg?1 exerted positive effects on growth and biochemical and hematological indices in rainbow trout.