Influence of various phosphopeptides of caseins on iron absorption

The influence of the origin and kind of caseinophosphopeptide (CPP) on iron absorption was assessed by comparing a commercially available CPP mixture (CPPs) and derived chromatographic fractions with the purified, chemically phosphopeptide of beta-casein [beta-CN(1-25)] using a perfused rat duodenal loop system; gluconate iron was used as control. Only iron complexed to beta-CN(1-25) displayed a better bioavailability than gluconate iron. The results obtained with various chromatographic fractions indicated that phosphopeptides of different origins (alpha(s)- versus beta-caseins) display specific effects. These findings contribute to the explanation of the discrepancy about the role of caseinophosphopeptides on mineral bioavailability in vivo.     

Use of heme iron concentrate in the fortification of weaning foods

The purpose of this study was to evaluate the technological feasibility of fortifying homogenized weaning food with a porcine heme concentrate. The stability of iron and the organoleptic qualities of two infant weaning foods (a commercial homogenized nonfortified weaning food, NFWF, and the same food fortified with 0.5% of porcine heme concentrate, FWF) were tested throughout 8 months of storage at room temperature and at 37 degrees C. Heme iron decreased with storage time; however, the proportion of this highly available iron was considerably higher in FWF than in NFWF. The addition of heme iron changed significantly the color of the weaning food measured instrumentally, although high temperatures and length of storage time, did not modify Lab, chroma, and hue angle values in both samples. Organoleptic attributes presented a marked stability even in NFWF stored at room temperature and 37 degrees C.     

Studies on absorption and hydrolysis of ethyl alpha-D-glucoside in rat intestine

Ethyl alpha-D-glucoside (alpha-EG) is normally contained in Sake, which has been taken by Japanese people since ancient times. In this study, the intestinal absorption of alpha-EG was investigated using rat everted intestinal sac. Furthermore, the alpha-EG hydrolytic activity in rat intestine was compared with disaccharides hydrolytic activities, and the effects of alpha-EG on disaccharides hydrolysis were examined using crude enzyme preparation from rat intestinal acetone powder. Glucose liberated from alpha-EG was detected in a serosal solution of everted rat intestinal sac, but it was only less than 4% of absorbed intact alpha-EG. alpha-EG absorption into small intestinal tissue was reduced by elimination of sodium ion from the mucosal solution or under the presence of phlorizin. The hydrolytic activity for alpha-EG was detected in crude enzyme preparation from rat intestinal acetone powder, but it showed a low value as compared to those for disaccharides. alpha-EG showed mixed type inhibition for maltose and sucrose hydrolysis, but inhibitory concentrations of alpha-EG required for 50% inhibition for the maltose and sucrose hydrolysis were higher than those of arabinose and acarbose. In conclusion, a small amount of alpha-EG was hydrolyzed and most of it was absorbed via SGLT1 as an intact form in the rat small intestine, and the inhibitory effect of alpha-EG on disaccharides hydrolysis was weak.     

Influence of components of infant formulas on in vitro iron, zinc, and calcium availability

The effect of casein content and Ca concentration on Fe, Zn, and Ca dialyzability was assessed using a response surface design. Tested casein levels were 5.31-13.75 g/L (34.8-90.2% of total protein). Whey protein was added to complete 15.25 g/L total protein. Calcium levels were adjusted with calcium citrate within a range between 417.4 and 804.9 mg/L. Through the experimental design utilized, we found that of both assessed factors, only the casein content significantly influenced Fe and Zn dialyzability. Protein composition did not influence calcium dialyzability, and calcium concentration did not affect either Fe or Zn dialyzability. No effect of casein-Ca on iron, zinc, and calcium dialyzability was found. According to these results, whey-dominant formulas are less prone to hamper mineral availability, and are therefore suitable in order to improve iron and zinc availability.     

Comparative physiological study of iron,manganese and copper absorption by plants

In the previous reports, influence of metabolic inhibitors, such as NaCN, 2,4-dinitrophenol and EDTA, on the absorption of ⁵⁹Fe, ⁵⁴Mn and ⁶⁴Cu(1) and the distribution of these elements in root cell were studied using the separation technique by centrifugal forces (2). These experiments revealed that rice and barley plants behave differently in their mode of absorption and distribution of these elements.     

Influence of ethanol content on the extent of copigmentation in a cencibel young red wine

The effect of ethanol content on the copigmentation of a Cencibel young red wine was studied, by means of ethanol elimination and reconstitution of the initial volume with different ethanol proportions. The reference wine (14.0% ethanol, 0.40 g/L volatile acidity) showed a bathochromic shift of 4 nm and a color enhancement (DeltaColor) of 41%, lower than that found for the reconstituted wine with the same ethanol content (53%). This discrepancy could be attributable to the loss of acetic acid during the ethanol elimination step. DeltaColor was 95% for the reconstituted wine without ethanol and decreased until 18% for the reconstituted wine with 22% ethanol. Copigmentation was important for reconstituted wines with ethanol contents typical for table red wine, showing DeltaColor between 53 and 57%. An increase in ethanol content in reconstituted wines was accompanied by an increase in the pH value. Perceivable changes in color (DeltaE > 1) followed every increase in ethanol content.     

Influence of genotype and processing on the in vitro rate of starch hydrolysis and resistant starch formation in peas (Pisum sativum L.).

The formation of resistant starch (RS) and the rate of starch hydrolysis were evaluated in vitro in a wild type of green-seeded pea genotype RRRbRb BC3 (33-Am) with 32.7% amylose content and in two mutants RRrbrb BC3 (23-Am) and rrRbRb BC3 (65-Am) with amylose contents of 23.3 and 65.1%, respectively. Pea samples were intact or homogenized and subjected either to autoclaving or to boiling at atmospheric pressure. The amount of RS (total starch basis) varied from 6.2 to 12.9% in the 23-Am products and from 31.2 to 33.4% in the 65-Am products. The RS level of the 33-Am product with a regular amylose content was 11.0%. Both the 23-Am and the 65-Am products were abundant sources of dietary fiber (39 and 34%, dry matter basis, respectively) versus 23% in the regular pea product. The amylose/amylopectin ratio was an important determinant of the rate of starch hydrolysis. The hydrolysis indices (HI) and predicted glycemic indices were lowest in the 65-Am peas (HI range = 42-59) as compared to the 23-Am peas (HI range = 53-84). It is concluded that the pea genotypes covered a wide range in starch availability, which is likely to affect nutritional parameters such as glycemic responses and colonic delivery of starch.     

Measurement of the bioavailability of iron, using the rat hemoglobin repletion test

Variations in the AOAC official first action rat hemoglobin repletion test for iron were studied. These changes included (1) use of a simplified basal diet to eliminate ingredients which sometimes contribute too much iron; (2) increased fortification of the basal diet with vitamin E, pantothenic acid, and pyridoxine; (3) increased dietary copper; (4) variations in the carbohydrate source in the basal diet; (5) changes in the length of the depletion and repletion periods; and (6) comparison of prophylactic and curative procedures. The changes yielded results comparable to those obtained with the present official method. Further study may reveal that the depletion period can be shortened or eliminated. To fully meet the rat's vitamin requirements, increased levels of vitamin E, pantothenic acid, and pyridoxine are recommended. It is further recommended that the present method remain in official first action status, and that study be continued.     

高产冬小麦铁素吸收分配特点的研究

采用干灰化——原子吸收分光光度计法 ,测定了田间高产试验条件下冬小麦不同生育时期植株地上部各器官的铁含量 .结果表明 :麦株含铁量随生育时期呈单峰曲线变化 ,其峰顶位于返青期 ;铁吸收量随生育的时期呈双峰曲线变化 ,其峰顶分别位于拔节期和乳熟期 ,而峰谷出现在开花期 ;各生育阶段麦株体内铁始终以叶片分配比率最高 ;形成 1 0 0kg籽粒需要吸收铁 1 7.98g .     

Comparative study on the effectiveness of several iron compounds in the iron chlorosis correction in citrus plants

A comparative study was carried out on the effectiveness of several commercial Fe‐compounds applied through the soil as well as via leaf spray, with a view to control the Fe‐chlorosis in Verna lemon trees directly grafted on sour orange rootstock, with a Salustiano orange tree as intermediate. The results obtained during 1985 confirm the conclusions of previous experiments: The most effective treatments were the leaf spray with Fe‐polyflavonoids, though it is interesting that Fe‐chelates applied to soil were also highly effective, as Fe leaf levels were higher than 100 ppm one month after treatments.     

Pro-oxidative characteristics of trout hemoglobin and myoglobin: a role for released heme in oxidation of lipids

The molecular mass of trout myoglobin was 16017 Da based on electrospray ionization mass spectrometry. A Root effect (low oxygen affinity at pH 6.3) was determined in trout hemoglobin but not myoglobin. At pH 6.3, myoglobin autoxidized more rapidly (3.5-fold) as compared to anodic hemoglobin. Anodic hemoglobin was a better catalyst of lipid oxidation in washed cod muscle as compared to myoglobin at pH 6.3. This suggested that some process other than met heme protein formation was the rate-limiting step in lipid oxidation processes. Heme loss rates were determined using the apomyoglobin mutant H64Y prepared from sperm whale. Anodic hemoglobin released its heme group much more rapidly than myoglobin. In comparisons of anodic and cathodic hemoglobins, heme loss rate better predicted the onset of lipid oxidation than autoxidation rate. These studies collectively suggest that heme dissociation has a primary role in the ability of different heme proteins to promote lipid oxidation processes.     

Influence of the leaf iron contents on the ferredoxin levels in citrus plants

Partial purification of the ferredoxin form crude extracts and their spectrophotometric quantification by means the measuring at 312 nm permit the determination of the ferredoxin levels in leaves of citrus plants. The leaf contents of this protein are related with the total iron concentrations, as well as with the iron chlorosis symptoms of the plants. On the other hand, iron infiltration treatments on intact leaves enhanced the sythesis of the ferredoxin     

Mechanisms of heme protein-mediated lipid oxidation using hemoglobin and myoglobin variants in raw and heated washed muscle

The hemoglobin variant rHb 0.1, which possesses a decreased ability to form subunits, stimulated lipid oxidation in washed fish muscle less effectively as compared to wild-type hemoglobin (rHb 0.0). This could be due to the lower hemin affinity and more rapid autoxidation rate of subunits as compared to tetramers. To differentiate between hemin affinity and autoxidation effects, ferrous V68T Mb was compared to ferrous wild-type myoglobin (WT Mb). WT Mb has a more rapid hemin loss rate (25-fold) than does V68T, while V68T autoxidized more rapidly than did WT Mb (60-fold). Ferrous WT Mb promoted TBARS and lipid peroxide formation more rapidly than did ferrous V68T (p < 0.01). This indicated hemin loss rate was more critical in determining onset of lipid oxidation as compared to autoxidation rate. Hemin alone was capable of stimulating lipid oxidation. Albumin enhanced the ability of hemin to promote lipid oxidation. MetMb promoted lipid oxidation more effectively than did ferrous Mb, which could be due to the lower hemin affinity of metMb as compared to that of ferrous Mb. EDTA, an iron chelator, had no effect on the rate or extent of lipid oxidation mediated by Mb in the cooked system. Variants with a 975-fold range of hemin affinities promoted lipid oxidation with equivalent efficacy in cooked washed cod contrary to results in uncooked washed cod. The cooking temperatures apparently denature the globin and release hemin reactant to such an extent that the impact of hemin affinity on lipid oxidation observed in the raw state is negated in the cooked state. These studies collectively suggest released hemin is of primary importance in promoting lipid oxidation in raw and cooked washed fish muscle.     

Influence of four levels of pyrophosphatase activity on the rate of pyrophosphate hydrolysis in organic soils

Abstract

In this study, the rate of pyrophosphate hydrolysis was measured in four organic soil materials similar in pyrophosphate retention values and in all other respects except pyrophosphatase activity. These soil materials had a bulk density of 0.20 kg/L and a pH (0.01 M CaCl₂) of 5.1, and contained 16% ash and 103 mg P/kg as Mehlich No. 1 extractable P. The difference in pyrophosphatase activity between the soil materials was attributed to response to Cu of previous crops at low values of 1 N HCl‐extractable Cu (10 vs 76 mg Cu/kg) and to pyrophosphatase inhibition by Cu at high Cu values (201 and 479 mg Cu/kg). Pyrophosphatase activity increased from 35.6 to 38.3 mmol/kg/(2h) with Cu contents increasing from 10 to 76 mg/kg, and decreased to 31.9 and 28.3 mmol/kg/ (2h) with Cu contents increasing respectively to 201 and to 479 mg/kg. However, no significant difference in course of pyrophos‐phate hydrolysis was observed among the soil materials under both laboratory and field conditions. The half‐life of pyrophosphate was nearly 1 day under laboratory conditions. The decrease in substrate concentration over time and the corresponding increase in products competing with substrate on active sites of enzymes tended presumably to mask the difference shown in pyrophosphatase activity among organic soil materials. Thus, a difference in pyrophosphatase activities larger than the one considered in this study would be required to influence the rate of pyrophosphate hydrolysis in organic soils of similar pyrophosphate retention characteristics. Such a difference in activity levels was not obtained despite a large difference in Cu contents among the soil materials. It was thus apparent that Cu contents, exceeding that generally found in Quebec organic soils, would not reduce the rate of orthophosphate production from polyphosphate fertilizers containing large amounts of pyrophosphate. Pyrophosphate hydrolysis would be rapid and not affected by a small reduction in pyrophosphatase activity.     

Effects of copper source and concentration on in vitro phytate phosphorus hydrolysis by phytase

Five copper (Cu) sources were studied at pH 2.5, 5.5, and 6.5 to determine how Cu affects phytate phosphorus (PP) hydrolysis by phytase at concentrations up to 500 mg/kg diet (60 min, 40-41 degrees C). Subsequently, Cu solubility with and without sodium phytate was measured. Adding Cu inhibited PP hydrolysis at pH 5.5 and pH 6.5 (P < 0.05). This inhibition was greater with higher concentrations of Cu. Tri-basic copper chloride and copper lysinate inhibited PP hydrolysis much less than copper sulfate pentahydrate, copper chloride, and copper citrate (P < 0.05). A strong negative relationship was observed between PP hydrolysis and soluble Cu at pH 5.5 (r = -0.76, P < 0.0001) and 6.5 (r = -0.54, P < 0.0001). In conclusion, pH, Cu concentration, and source influenced PP hydrolysis by phytase in vitro and were related to the amount of soluble Cu and the formation of insoluble copper-phytin complexes.     

Determination of iron in soils by flow injection atomic absorption spectrometry

Abstract

A single‐channel flow injection system was optimized for the determination of available iron (Fe) in soil extracts by atomic absorption spectrophotometry. This method of introducing the samples in the spectrophotometer worked particularly well in preventing blockage of the burner head which was observed in the conventional introduction of Fe for its determination by atomic absorption spectrophotometry. The appropriate selection of the manifold parameters, such as injection volume, tube length and flow rate, allowed introduction of any soil extract without requiring any pre‐treatment. This system allowed determinations at a detection limit of 0.36 mg L^‐1 to 5 mg L^‐1, with an output of 300 determinations per hour. The results obtained for analysis of 15 soil extracts were in good agreement with those provided by the colorimetric method, with average relative deviations of 1.6%. Relative standard deviations of 4.8, 2.5, and 2.3% were obtained for contents of 1.03, 1.85, and 3.99 mg Fe L^‐1, respectively.     

Influence of iron oxides on cobalt adsorption by soils

Eleven soils from Denmark and Tanzania were extracted with ammonium acetate (controls), EDTA, and dithionite-EDTA (DE) to fractionate iron (and manganese) oxides. The amounts of cobalt adsorbed and acidity desorbed by the extracted soils as well as by two synthetic iron oxides were determined, using 0.85 mM cobalt in 0.2 m NaNO₃.
No significant correlations were found between cobalt adsorption and the contents of extractable manganese or organic matter, presumably because of their low contents. The major part of the cobalt adsorption (by DE-extracted samples) was due to the clay fraction and was associated with the release of approximately one proton per adsorbed Co. The remaining cobalt adsorption was attributed to the iron oxides. This portion of adsorbed cobalt was well described by considering soil iron oxides composed of only two fractions, an EDTA-extractable fraction of high reactivity and a less reactive fraction corresponding to the difference between DE-extractable iron and EDTA-extractable iron. Approximately 1.7 protons were released per Co adsorbed by these iron oxide fractions and by the synthetic iron oxides.
The amounts of cobalt adsorbed by the soil iron oxides were well predicted from the contents and specific surfaces of the two iron oxide fractions together with the specific cobalt adsorption of synthetic iron oxides.