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鸡新城疫二价油乳剂灭活苗的研制及免疫效果 总被引:3,自引:0,他引:3
用NDLasota弱毒株、NDV地方分离株(F基因Ⅶ型)-HD1,分别接种鸡胚,收集尿囊液,制备病毒抗原液,福尔马林灭活后按一定比例混合,与白油佐剂乳化成油包水型乳剂疫苗,并对二价苗各项指标进行了测定,证明本疫苗安全、无副作用,接种后3周产生免疫保护,攻毒保护率为100%,接种2周后通过免疫抗体检测,NDHI抗体效价达8log2,疫苗在鸡场应用,获得了满意效果,有效的控制了ND的发生与流行。 相似文献
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对张家口市奶牛场进行了奶牛乳腺炎调查并对25头患有急性乳腺炎奶牛的64个乳区64份乳样进行细菌分离与鉴定,共分离葡萄球菌、链球菌、沙门氏菌、棒状杆菌、大肠杆菌、酵母菌6种致病菌,其中,酵母菌最多,占32.81%,葡萄球菌和链球菌之和占40.63%. 相似文献
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哈尔滨地区奶牛隐性乳房炎病原菌的分离鉴定 总被引:3,自引:0,他引:3
通过BMT法、乳汁pH检验法和体细胞直接计数法等方法相结合,对哈尔滨市3个大型奶牛场、10户奶牛养殖户选取的1 000头奶牛,4000个乳区进行隐性乳房炎的流行病学调查,以及致病菌的分离鉴定.结果表明,其中隐性乳房炎的发病率为47.7%(477/1 000);乳区发病率为27.3%(1092/4000),从477头的隐... 相似文献
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为了解浙江省奶牛乳房炎致病菌的耐药情况,评估其耐药表型与基因型的相关性,本研究从浙江省主要奶牛养殖区奶牛乳房炎病例分离到70株金黄色葡萄球菌,测定它们对13种常用抗菌药物的药敏性,并采用聚合酶链式反应(PCR)检测这些细菌耐青霉素G、庆大霉素、红霉素和四环素的耐药基因mecA、aac(6')/aph(2″)、erm和tetM的携带情况.结果表明,奶牛乳房炎金黄色葡萄球菌对常用抗生素的耐药情况严重,耐药表型和耐药基因型的符合率较高.70株金黄色葡萄球菌主要对青霉素G、庆大霉素、红霉素和四环素耐药,表型耐药率分别为90.0%、67.1%、50.0%和60.0%,耐药基因mecA、aac(6')/aph(2″)、erm和tetM的检出率分别是92.9%、77.1%、48.6%和61.4%,耐药表型与耐药基因型的符合率分别为97.1%、70.0%、87.1%和81.4%. 相似文献
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对奶牛隐性乳房炎乳汁中体细胞、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、谷氨酸草酰乙酸转氨酶(GOT)、谷氨酸丙酮酸转氨酶(GFT)和血清白蛋白(SA)及病原微生物的相关性进行了研究。结果发现LDH,ACP,GOT,GPT的活性和sA的含量变化与白细胞数之间存在极显著的相关关系(P<0.01),尤以LDH的相关性最强(r=0.858);ALP与体细胞数之间无相关关系(P>0.05).不同的乳房炎病原微生物感染乳腺呈现不同的酶象变化,与阴性感染的乳腺相比,各酶的活性均出现增加,尤以金黄色葡萄球菌感染的乳腺有关酶的增加幅度最为显著(P<0.01)。 相似文献
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Detection of antimicrobial resistance and virulence-related genes in Streptococcus uberis and Streptococcus parauberis isolated from clinical bovine mastitis cases in northwestern China 下载免费PDF全文
ZHANG Hang YANG Feng LI Xin-pu LUO Jin-yin WANG Ling ZHOU Yu-long YAN Yong WANG Xu-rong LI Hong-sheng 《农业科学学报》2020,19(11):2784-2791
The objectives of this study were to investigate antimicrobial resistance of Streptococcus uberis and Streptococcus parauberis isolated from cows with bovine clinical mastitis in China and to examine the distribution of resistance- and virulence-related gene patterns. Antimicrobial susceptibility was determined by the E-test. Genes encoding antimicrobial resistance and invasiveness factors were examined by PCR. A total of 27 strains were obtained from 326 mastitis milk samples. Streptococcus parauberis isolates (n=11) showed high resistance to erythromycin (90.9%), followed by tetracycline (45.5%), chloramphenicol (36.4%) and clindamycin (27.3%). Streptococcus uberis isolates (n=16) were highly resistant to tetracycline (81.3%) and clindamycin (62.5%). Both species were susceptible to ampicillin. The most prevalent resistance gene in S. uberis was tetM (80.0%), followed by blaZ (62.5%) and ermB (62.5%). However, tetM, blaZ, and ermB genes were only found in 27.3, 45.5, and 27.3%, respectively, of S. parauberis. In addition, all of the isolates carried at least one selected virulence-related gene. The most prevalent virulence-associated gene pattern in the current study was sua+pauA/skc+gapC+hasC detected in 22.2% of the strains. One S. uberis strain carried 7 virulence-associated genes and belonged to the sua+pauA/skc+gapC+cfu+hasA+hasB+hasC pattern. More than 59.3% of analysed strains carried 4 to 7 virulence-related genes. Our findings demonstrated that S. parauberis and S. uberis isolated from clinical bovine mastitis cases in China exhibited diverse molecular ecology, and that the strains were highly resistant to antibiotics commonly used in the dairy cow industry. The data obtained in the current study contribute to a better understanding of the pathogenesis of bacteria in mastitis caused by these pathogens, and the findings are relevant to the development of multivalent vaccines and targeted prevention procedures. 相似文献
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奶牛乳腺炎调查及病因分析 总被引:1,自引:0,他引:1
对佳木斯市4所奶牛场进行流行病学调查,研究临床型乳腺炎和隐性乳腺炎的发病率以及病因。对4所奶牛场的390头泌乳牛进行检测,其中218头患有隐性型乳腺炎,头发病率为55.90%;17头患有临床型乳腺炎,头发病率为4.43%;乳腺炎的头发病率为60.26%。在被检的1416个有效乳区中,患有隐性型乳腺炎的乳区有792个,乳区阳性率为55.93%;患有临床型乳腺炎的乳区有20个,乳区阳性率为1.41%。对牛场圈舍条件和生产、管理诸环节调查分析表明,引起奶牛乳腺炎的主要原因为饲养管理水平较低、饲料品质较低、挤奶工不能严格遵守无菌操作、高产奶牛乳头未开展干奶期预防工作等。 相似文献
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Ji-tao CHANG De-bin YU Jian-bin LIANG Jia CHEN Jian-fa WANG Fang WANG Zhi-gang JIANG Xi-jun HE Rui WU Li YU 《农业科学学报》2019,18(1):160-168
Twelve quarters of six lactating cows were inoculated with Mycoplasma leachii strain GN407 through intramammary ductal infusion; another 12 quarters were inoculated with heat-inactivated M. leachii culture medium as negative controls. Multidisciplinary procedures, including clinical assessment, etiology assessment, pathology and immunohistochemistry (IHC), were used to elucidate the pathogenicity of M. leachii in bovine mastitis. From post-inoculation days (PIDs) 3 to 9, 12 inoculated quarters developed mild to severe clinical mastitis and mammary tissue histopathological changes, including inflammatory cell infiltration and architectural destruction of mammary gland ducts. The M. leachii antigen was also detected by IHC in the mammary gland epithelial cells of the inoculated quarters as a weak signal on PID 6 and as a strong signal on PID 9. The control quarters also developed mild mastitis and histopathological changes on PID 9, and M. leachii was also detected by IHC. Throughout the experimental period, the quarters of the negative control cow were clinically and pathologically normal, and the M. leachii antigen was not detected. In conclusion, direct histological and immunohistochemical evidence confirmed that M. leachii causes clinical bovine mastitis through histopathological lesions induced by invasion of the pathogen into mammary gland cells and through inflammatory cell infiltration. 相似文献
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对佳木斯市4所大型奶牛场17头患有临床型乳腺炎的奶牛的不同乳区的20份乳汁样品进行致病菌的分离鉴定,并对分离得到的致病菌进行8种氟喹诺酮类药物和3组中药方剂的敏感性试验。结果表明,从被检乳汁样品中共分离得到致病菌508株,包括金黄色葡萄球菌、表皮葡萄球菌、无乳链球菌、乳房链球菌和化脓链球菌,分别占致病菌总数的35.43%、10.63%、39.96%、11.22%、2.76%。药敏试验表明,分离得到的致病菌对8种氟喹诺酮类药物均敏感,其中金黄色葡萄球菌对恩诺沙星最敏感,无乳链球菌对加替沙星最敏感;3组中药方剂中以方剂Ⅰ的抑菌效果最好。 相似文献
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基因治疗奶牛乳腺炎的生物安全评价 总被引:1,自引:0,他引:1
为评价基因治疗奶牛乳腺炎的生物安全性,以3头患隐性乳腺炎的奶牛作为试验对象,通过乳房基部穿刺注射接种携带人溶菌酶基因的乳腺特异性表达重组质粒p215C3LYZ(1 600μg·头~(-1)),24 h后以相同剂量重复注射1次.定期采集试验奶牛的乳样、粪样、尿样以及试验牛舍的垫料和水样,并利用建立的PCR进行检测.结果发现,除第2次注射24 h内可从奶样中检测到重组质粒外,其他样品均为阴性,这表明重组质粒p215C3LYZ不能在奶牛体内长期残留,也不能在环境中释放及散播.所有奶牛均于第2次注射20 d后处死,并采集血液、心、肝,脾、肺、肾、肌肉以及乳腺等样品进行PCR检测,结果表明:重组质粒p215C3LYZ在奶牛体内未发生转移. 相似文献
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胚期接种IBD疫苗对雏鸡ND常规免疫效果之影响 总被引:1,自引:1,他引:1
为探明用IBD疫苗进行鸡胚免疫的可行性,探讨了胚期接种IBD疫苗对雏鸡ND常规免疫接种效果的影响.将IBD中等毒力疫苗接种18日龄鸡胚,出壳后按常规免疫方法进行ND免疫,检测抗体水平、免疫细胞数量及功能和免疫保护力等.结果表明,胚期接种IBD中等毒力疫苗对雏鸡ND常规免疫具有免疫抑制作用. 相似文献
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奶牛乳铁蛋白基因部分序列的SSCP分析及其与乳房炎的关系 总被引:3,自引:0,他引:3
乳房炎是奶牛生产中最普遍、最复杂也是花费最多的疾病,而乳铁蛋白在机体抵抗乳房炎方面起着重要作用.从523头泌乳奶牛中,以临床乳房炎发病记录为基础,分别筛选出40头具有最高抗性和最高易感性的奶牛作为试验I组和试验II组.同时对bLF启动子和第4、15外显子做PCR-SSCP分析,在6个亚片断上,发现P1,P4,E4,E15等4个DNA片断存在多态,测序后发现6个突变位点.对4个DNA多态片断做进一步的分析,发现P1、P4片断的等位基因在试验两组中的分布呈显著差异,分别为53.7% vs 70.0%(P<0.05),55.0% vs 68.8%(P<0.05),可作为潜在的分子标记.结合试验奶牛的生产记录分析了分子标记的相关影响,结果发现筛选将引起奶牛乳脂率显著上升(P<0.05),体细胞数显著下降(P<0.05),CMR值极显著差异(P<0.01)和产奶量显著下降(P<0.05).同时还利用MatInspector7.2.2 软件对突变引起的启动子区潜在转录因子结合位点的改变进行了研究,并预测了乳铁蛋白表达调节的两个可能的信号转导通路即NF-κB炎症响应途径和核激素受体途径. 相似文献