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从青海省海晏县发生胸膜肺炎的绵羊群中采集3份肺组织病料,进行病原的分离培养和特异性PCR检测。结果从3份肺组织中均分离到支原体,经鉴定为绵羊肺炎支原体,表明建立的PCR方法能对绵羊肺炎支原体做出正确诊断。 相似文献
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山羊支原体性肺炎流行病学调查 总被引:2,自引:1,他引:1
山羊支原体性肺炎是威胁山羊养殖的重要传染病,为了解其流行情况,对四川省主要山羊养殖地区的山羊支原体性肺炎进行了流行病学调查。从四川省7个地区山羊养殖场采集肺脏和鼻腔棉拭子样本共135份,经过分离鉴定得到42株支原体,其中绵羊肺炎支原体36株,丝状支原体6株;其中6个羊场仅分离到绵羊肺炎支原体,1个羊场同时分离到绵羊肺炎支原体和丝状支原体。本试验结果表明,绵羊肺炎支原体是引起四川省山羊支原体性肺炎的主要病原,个别地方存在绵羊肺炎支原体和丝状支原体混合感染。 相似文献
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随着养兔业的不断发展,家兔呼吸道疾病越来越引起人们的重视。江苏省农科院畜牧兽医研究所毛洪先等于1991年从兔肺炎病灶分离到支原体,为国内外首次分离和培养成功,并初步作了鉴定,1993年又从毛兔活体分离到兔肺炎支原体,证明该病原在兔群中有感染和流行。为了深入研究兔肺炎支原体的体外生长特性,寻找治疗兔肺炎支原体病的方法,我们采用3种不同的培养液,从某兔场分离培养兔肺炎支原体病原,并进行传代,同时对该病进行了药物治疗试验。现将有关情况报告如下:1 材料与方法1.1 分离培养液牛肝汤培养液:自制牛肝汤培… 相似文献
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为探索SPF鸡胚作为绵羊肺炎支原体实验室感染模型的可行性,本试验用不同浓度绵羊肺炎支原体(108、109、1010 ccu/mL)经由卵黄囊和尿囊腔两个部位接种7日龄SPF鸡胚,通过统计鸡胚死亡情况和不同鸡胚组织样品中绵羊肺炎支原体检测阳性率(PCR检测和支原体分离鉴定),确定绵羊肺炎支原体鸡胚感染方式、感染剂量和最佳分离部位,再用3株不同来源的绵羊肺炎支原体分离株感染鸡胚,观察其对鸡胚的致病力。结果表明,绵羊肺炎支原体感染鸡胚最佳接种途径为卵黄囊接种,感染剂量为109 ccu/mL、0.2 mL/只,最佳分离部位为卵黄液。3株支原体均能感染和致死鸡胚,并均能从卵黄液中分离到绵羊肺炎支原体,但对鸡胚的致病性存在一定差异:FL3株致鸡胚死亡率为45%,略高于MoGH3-3株(40%),二者均高于A3株(25%),但差异均不显著(P>0.05);FL3株鸡胚检测阳率为100%,高于MoGH3-3株(85%)及A3株(90%),但差异也不显著(P>0.05)。本试验初步确定了绵羊肺炎支原体可感染和致死SPF鸡胚,不同分离株对SPF鸡胚致病力有差异,表明SPF鸡胚可作为下一步建立绵羊肺炎支原体实验室感染模型的候选,为绵羊肺炎支原体致病性研究和疫苗研制奠定基础。 相似文献
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试验采用SDS—PAGE和免疫印迹分析技术研究了绵羊肺炎支原体标准株Y98与绵羊肺炎支原体分离株HD-1、丝状支原体丝状亚种PG3以及肺炎支原体标准株FH间细胞膜蛋白免疫原性的异同。结果表明:绵羊肺炎支原体标准株Y98同绵羊肺炎支原体分离株HD-1间细胞膜蛋白免疫印迹结果基本一致,而绵羊肺炎支原体标准株Y98与丝状支原体丝状亚种PG3和肺炎支原体标准株FH抗原差异较大,缺乏共同抗原成分。 相似文献
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通过对送检病羊的临床症状、剖检变化、病科染色镜检,初步诊断为绵羊支原体肺炎。又经无茵采取病料接种改良Frey培养氏液,再取培养液接种于固体培养液后分离到可疑绵羊肺炎支原体。然后将培养生长的支原体分别进行革兰氏染色、姬姆萨染色、瑞氏染色和碱性复红染色;进行了各种生化试验,进行动物和鸡胚接种试验和平扳凝集等血清学试验;最后确诊为绵羊支原体肺炎,并分离获得了绵羊肺炎支原体。该结果为绵羊支原体肺炎的防制奠定了基础。 相似文献
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通过对送检病羊的临床症状、剖检变化、病料染色镜检,初步诊断为绵羊支原体肺炎。又经无菌采取病料接种改良Frey培养氏液,再取培养液接种于固体培养液后分离到可疑绵羊肺炎支原体。然后将培养生长的支原体分别进行革兰氏染色、姬姆萨染色、瑞氏染色和碱性复红染色;进行了各种生化试验,进行动物和鸡胚接种试验和平扳凝集等血清学试验;最后确诊为绵羊支原体肺炎,并分离获得了绵羊肺炎支原体。该结果为绵羊支原体肺炎的防制奠定了基础。 相似文献
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A J DaMassa E R Nascimento M I Khan R Yamamoto D L Brooks 《Journal of veterinary diagnostic investigation》1991,3(1):55-59
A mycoplasma designated strain GM790A was isolated from milk and internal organs of 2 lactating goats showing mastitis and arthritis. The isolate was not related serologically to any of the currently known ovine-caprine mycoplasmas, except an isolate designated Mycoplasma sp. G, first recorded from the external ear canal of clinically normal goats in Australia. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restriction enzyme DNA studies of strain GM790A and Mycoplasma sp. G revealed similar but not identical patterns. The inoculation of strain GM790A into the teat canal of 2 lactating goats resulted in an abrupt diminution of lactation leading to mastitis and agalactia in about 3 days. A maximum of 1 x 10(7) colony-forming units (CFU) of the mycoplasma were shed per ml of mammary secretion. Milk production partially resumed at a low level 3 weeks postinoculation, the longest period tested, but the milk still contained 1 x 10(2) CFU of the agent. The results of this study indicate that strain GM790A possesses pathogenic potential for the goat and most probably represents a new species of the genus Mycoplasma. 相似文献
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A mycoplasma identified asMycoplasma mycoides subspmycoides LC type was isolated from an outbreak of caprine pleuropneumonia and serofibrinous peritonitis. This isolate was the first to be reported in Italy. Experimentally, a rapidly fatal condition followed its inoculation into goats and sheep but inoculation of calves did not lead to clinically apparent infection. Although the organism was recovered for up to 45 days following experimental inoculation of calves, pathological changes were limited to the drainage lymph nodes except in one case where there were histological changes in the lungs. 相似文献
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Pleuropneumonia was induced in goats by inoculation of mycoplasma strain ... SGP1 (F38 type) and spread readily from them to in-contact goats which in turn passed it to other in-contact goats. This emphasises the similarity between the experimentally produced disease and classical contagious caprine pleuropneumonia (CCPP) thus supporting the view that SGP1 has an aetiological role in CCPP in the field. 相似文献
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Isolation of Mycoplasma mycoides subspecies mycoides from Polyarthritis and Mastitis of Goats in Canada 总被引:3,自引:0,他引:3
Ruhnke HL Rosendal S Goltz J Blackwell TE 《The Canadian veterinary journal. La revue veterinaire canadienne》1983,24(2):54-56
The clinical signs, pathomorphological changes, and microbiological findings in Canadian goats infected with Mycoplasma mycoides subspecies mycoides are discussed. The disease affected mainly young goats and was characterized by septicemia and polyarthritis. Mastitis followed by septicemia was seen in two mature goats. The diagnosis was made by culture and identification of the mycoplasma. Infected goats without clinical signs were identified by cultural and serological (complement fixation) techniques. Healthy carriers are presumably able to transmit the infection and may have brought the disease to Canada. 相似文献
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Biological effects of sonicated suspension and phenol-water extract of Mycoplasma mycoides subspecies mycoides in goats.
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A Valdivieso-Garcia S Rosendal M G Maxie 《Canadian journal of veterinary research》1982,46(2):190-197
Strain Y3343 isolated from a goat with septicemia and polyarthritis was studied. The strain was virulent and induced septicemia, polyarthritis and coagulopathy in two goats. Limulus amebocyte lysate active material was present in plasma, but not in higher titre in inoculated goats. Sonicated mycoplasma material induced a dramatic somatic cell response in the mammary gland of cows and goats and marked clotting of the cows' milk, but it did not clot limulus amebocyte lysate or kill chick embryos. Phenol-water extract clotted limulus amebocyte lysate and induced somatic cell response in cows but not in goats. The phenol-water extract did not kill chick embryos, was not pyrogenic in rabbits or goats, and did not induce generalized Shwartzman reaction or change the leukocyte kinetics in rabbits. It therfore appears that the virulence mechanisms of strain Y3343 can not be explained on the basis of factors with strong endotoxin activity. 相似文献
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Kusiluka LJ Ojeniyi B Friis NF Kazwala RR Kokotovic B 《Acta veterinaria Scandinavica》2000,41(3):299-309
A microbiological study of the mycoplasma flora in the respiratory tracts of cattle and goats in selected regions of Tanzania is described. In the examination of cattle, mycoplasmas were isolated from 60 (17.8%) of the 338 examined lung samples, 8 (47.1%) of the 17 lymph nodes, 4 (13.3%) of the 30 pleural fluid samples and 4 (3.9%) of the 103 nasal swabs examined. All the isolates were identified as Mycoplasma mycoides subsp. mycoides, Small Colony type except for one isolate from pleural fluid which was identified as Mycoplasma arginini. M. mycoides subsp. mycoides, Small Colony type was isolated from samples originating from Dodoma, Iringa, Mbeya, Morogoro and Shinyanga regions where outbreaks of contagious bovine pleuropneumonia had been reported. In the examination of goats, mycoplasmas were isolated from 54 (34.0%) of the 159 examined lung samples, 41 (18.1%) of the 226 nasal swabs and 4 (40.0%) of the 10 pleural fluid samples. The species demonstrated were Mycoplasma capricolum subsp. capripneumoniae, M. mycoides subsp. mycoides, Small Colony type Mycoplasma ovipneumoniae and M. Capricolum subsp. arginini. The isolation of M. capripneumoniae in the Coast and Morogoro regions confirmed the presence of contagious caprine pleuropneumonia in the regions. 相似文献
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Mycoplasma mycoides subsp. capri associated with goat respiratory disease and high flock mortality 总被引:5,自引:0,他引:5
Hernandez L Lopez J St-Jacques M Ontiveros L Acosta J Handel K 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(4):366-369
A high mortality outbreak of respiratory mycoplasmosis occurred in goats in Mexico. The clinicopathologic presentation resembled contagious caprine pleuropneumonia caused by Mycoplasma capricolum subspecies capripneumoniae. By using a battery of polymerase chain reaction assays, the mycoplasma associated with this outbreak was identified as Mycoplasma mycoides subsp. capri. 相似文献