Harmonease Chewable Tablets reduces noise-induced fear and anxiety in a laboratory canine thunderstorm simulation: A blinded and placebo-controlled study

Thunderstorm simulation in the laboratory setting induces fearful and anxious behavior in beagles, most notably manifested by increased inactivity (“freezing”), which, in a companion study, was ameliorated by the anxiolytic diazepam (Araujo et al., 2009). Using this protocol, the present study assessed the efficacy of Harmonease, a chewable oral anxiolytic botanical product containing a proprietary blend of extracts of Magnolia officinalis and Phellodendron amurense. A balanced, placebo-controlled, blinded, single crossover design including 20 healthy adult beagles was used for this study. After a baseline thunderstorm test, subjects received Harmonease Chewable Tablets or placebo treatment daily and were reassessed on the treatment day 7. After a 7-day washout period, the treatments were crossed over and a design identical to that used in the first phase was used. The thunderstorm test was performed in an open-field arena (8 ft × 9 ft) and consisted of three 3-minute phases: an anticipatory phase in which no stimulus was provided; the thunderstorm phase in which a thunderstorm track was played over a speaker system; and a recovery phase in which no stimulus was presented. Inactivity duration was considered the primary variable for assessing efficacy which was measured by a trained observer. Difference in number of dogs improved versus worsened by treatment group was significant at P < 0.05. Specifically, 12 of 20 (60%) dogs improved from baseline when treated with Harmonease, whereas only 5 of 20 (25%) improved on placebo. Furthermore, 9 of 20 (45%) placebo dogs showed increased inactivity duration (worsened), whereas only 4 of 20 (20%) treatment dogs worsened. Increases in distance travelled consistent with reduced inactivity were also seen under Harmonease. Harmonease reduced fear-related inactivity or freezing in dogs in this thunderstorm simulation model. This supports past studies demonstrating that the combination of botanical extracts in Harmonease is effective in dogs for the management of stress-related behaviors.     

ANXITANE® tablets reduce fear of human beings in a laboratory model of anxiety-related behavior

Anxiety and fear are common underlying factors in many canine behavior problems that impair the human–pet bond and often result in abandonment, relinquishment, or euthanasia. A combination of behavioral and pharmacological interventions is used to ameliorate the behavioral signs associated with anxiety-related behaviors in dogs, but there continues to be need for effective interventions. The current study examined the effects of the nutraceutical ANXITANE^® (l-Theanine) chewable tablets on fear of unfamiliar human beings. We first characterized dogs as anxious on the basis of the existence of a fear response to human beings in their home-pen. We then demonstrated that dogs characterized as anxious (N = 10) showed reduced interaction with an unknown human being as compared with normal controls (N = 7). The effect of an administration of ANXITANE^® tablets (N = 5) on these anxious Beagle dogs was compared with placebo (N = 5). Objective behavioral measures of anxiety were obtained using an open-field test, a human interaction test, and an actiwatch protocol that allowed monitoring of activity over 24-hours. The ANXITANE^® tablets-treated dogs showed greater human interaction and approach than the placebo control group, and no side effects related to treatment, including motor stimulant or sedative effects, were seen. The current study suggests that ANXITANE^® tablets are effective for reducing fearful behavior toward unfamiliar human beings in dogs and supports their use for treating anxiety-related behaviors.     

A review of pre-appointment medications to reduce fear and anxiety in dogs and cats at veterinary visits

This review focuses on pre-appointment medications used to decrease fear and anxiety in dogs and cats related to veterinary visits. A review of the literature revealed data on 4 medications from 4 medication classes that have been used to ameliorate acute situational fear and anxiety in dogs and cats: gabapentin, trazodone, oral transmucosal dexmedetomidine, and alprazolam. The available information on use, mechanism of action, and pharmacokinetics is reviewed.     

Evaluation of a behavioral assessment questionnaire for use in the characterization of behavioral problems of dogs relinquished to animal shelters

OBJECTIVE: To evaluate a behavioral intake questionnaire in animal shelters for the presence of biased results and assess its use in the characterization of behavioral problems of dogs relinquished to shelters. DESIGN: Cross-sectional study. Animals-54 dogs being relinquished to a shelter and 784 dogs belonging to veterinary clients. Procedure-Owners who were relinquishing their dogs and agreed to complete the behavioral questionnaire were alternately assigned to 1 of 2 groups; participants were aware that information provided would be confidential or nonconfidential (i.e., likely used for adoption purposes). Data from confidential and nonconfidential information groups were compared, and the former were compared with data (collected via the questionnaire) regarding a population of client-owned dogs. RESULTS: Analyses revealed significant differences in 2 areas of reported problem behavior between the confidential and nonconfidential information groups: owner-directed aggression and stranger-directed fear. Compared with client-owned-group data, significantly more relinquished shelter dogs in the confidential information group were reported to have owner-directed aggression, stranger-directed aggression, dog-directed aggression or fear, stranger-directed fear, nonsocial fear, and separation-related behaviors. CONCLUSIONS AND CLINICAL RELEVANCE: Among persons relinquishing dogs to a shelter, those who believed questionnaire responses were confidential reported owner-directed aggression and fear of strangers in their pets more frequently than relinquishers who believed responses were nonconfidential. Confidentiality had no apparent effect on the reporting of other assessed behavioral problems. Results suggest that behavioral questionnaires may sometimes provide inaccurate information in a shelter setting, but the information may still be useful when evaluating behavior of relinquished dogs.     

AAFP and ISFM feline-friendly handling guidelines

BACKGROUND: The number of pet cats is increasing in most countries, often outnumbering pet dogs, yet cats receive less veterinary care than their canine counterparts.(1) Clients state the difficulty of getting the cat into a carrier at home, driving to the clinic, and dealing with the fearful cat at the veterinary clinic as reasons for fewer visits.(2) Educating and preparing the client and the veterinary team with regard to respectful feline handling is necessary in order to avoid stress and accomplish the goal of good health care. Without such preparation, feline stress may escalate into fear or fear-associated aggression. The resulting stress may alter results of the physical examination and laboratory tests, leading to incorrect diagnoses (eg, diabetes mellitus) and unnecessary treatments.(3-5) Without compassionate and respectful handling by the veterinary team, clients may feel the team lacks skills and compassion, or does not understand cats. Injury may occur to the cat, client and/or veterinary team.(6) Clients who want to avoid stress for their cat may avoid veterinary visits or choose another practice instead. GOALS: The use of feline-friendly handling techniques should reduce these problems. Handling is most successful when the veterinary team adapts the approach to each individual cat and situation. The goal of these guidelines is to provide useful information for handling cats that can lead to: ? Reduced fear and pain for the cat. ? Reinforced veterinarian-client-cat bond, trust and confidence, and thus better lifelong medical care for the cat. ? Improved efficiency, productivity and job satisfaction for the veterinary team. ? Increased client compliance. ? Timely reporting and early detection of medical and behavioral concerns. ? Fewer injuries to clients and the veterinary team. ? Reduced anxiety for the client.     

Efficacy of dog-appeasing pheromone (DAP) for ameliorating separation-related behavioral signs in hospitalized dogs

Dogs hospitalized in veterinary clinics are likely to show signs of separation-induced anxiety from hospitalization. The study assessed the effect of dog-appeasing pheromone (DAP) on 10 typical separation-related behavioral signs in hospitalized dogs. A DAP treated group (n = 24) was compared with a placebo control group (n = 19). There was overall amelioration of the signs without ‘vigilance’ and ‘anorexia’ in the DAP-treated dogs; marked decreases were noted in elimination (P = 0.038), excessive licking (P = 0.005), and pacing (P = 0.017). The results suggest that the use of DAP could decrease separation-induced anxiety, distress, and fear in inpatients, and possibly facilitate recovery in hospitalized dogs.     

Effects of alpha-casozepine (Zylkene) versus selegiline hydrochloride (Selgian,Anipryl) on anxiety disorders in dogs

After a first study showing efficacy on anxiety disorders in cats, the putative effects of alpha-casozepine (a tryptic bovine αs1-casein hydrolysate) on anxious disorders in dogs was investigated. The trial was conducted against a control molecule, selegiline. Thirty-eight dogs were recruited within veterinary practices by certified behaviorist surgeons. This 56 day trial, against the reference molecule, selegiline, showed that both products were efficient to decrease the EDED score and no statistical difference was found between their success score. Owners assessment was also statistically perfectly equivalent. Due to this efficacy, and to its safety, alpha-casozepine (Zylkene) should be considered an option by the veterinary surgeon for the biological management of anxiety beside the compulsory behavior modifications.     

Use of fluoxetine,diazepam, and behavior modification as therapy for treatment of anxiety-related disorders in dogs

The objective of this study was to evaluate the use of fluoxetine, diazepam, and behavior modification for the treatment of a variety of anxiety disorders in dogs of different breeds, ages, and sexes that presented to a university veterinary behavior clinic. Forty dogs were enrolled in the study, and 34 completed it. The dogs were grouped into 2 major diagnostic categories, aggressive and anxious, according to the presenting signs. Moreover, the dogs further subdivided into 4 groups: neutered, intact, juveniles, and adults. Diazepam was administered orally, at a dosage of 0.3 mg/kg once a day for 4 weeks; fluoxetine was administered orally at a dosage of 1 mg/kg once a day for 10 weeks. The drugs were started simultaneously. The owners were given a behavior modification plan for their dogs that started from the first week of therapy. The behavior of the subjects was evaluated at 3 points in time. Clinical response was assessed by the supervising veterinarian and the owners. There were no significant differences (P > 0.05) between the groups studied in relation to the improvement achieved. There was a positive correlation (r > 0.44) between owner compliance with the treatment and the values obtained for the improvement achieved for each period. Clinical signs largely improved or were eliminated in 13 dogs (38%), 8 dogs (26%) showed moderate improvement, 5 dogs (12%) were slightly improved, and there were 8 dogs (24%) with no changes in clinical signs.     

Frequency of nonspecific clinical signs in dogs with separation anxiety, thunderstorm phobia, and noise phobia, alone or in combination

OBJECTIVE: To determine the frequency of nonspecific clinical signs in dogs with separation anxiety, thunderstorm phobia, noise phobia, or any combination of these conditions and determine whether these conditions are associated in dogs. DESIGN: Case series. ANIMALS: 141 dogs. PROCEDURE: Diagnoses were established using specific criteria. Owners of dogs completed a questionnaire on how frequently their dogs exhibited destructive behavior, urination, defecation, vocalization, and salivation when the owners were absent and the types and frequency of reactions to thunderstorms, fireworks, and other noises. RESULTS: Associations of the 3 conditions and of various nonspecific clinical signs within and between diagnoses were nonrandom. The probability that a dog would have separation anxiety given that it had noise phobia was high (0.88) and approximately the same as the probability it would have separation anxiety given that it had thunderstorm phobia (0.86). However, the probability that a dog would have noise phobia given that it had separation anxiety (0.63) was higher than the probability that it would have thunderstorm phobia given that it had separation anxiety (0.52). The probability that a dog would have noise phobia given that it had thunderstorm phobia (0.90) was not equivalent to the converse (0.76). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that dogs with any of these conditions should be screened for the others. Interactions among these conditions are important in the assessment and treatment of dogs with > 1 of these conditions. Responses to noise were different from those to thunderstorms, possibly because of the unpredictability and uncertainty of thunderstorms.     

Effects of prescription diet on dealing with stressful situations and performance of anxiety-related behaviors in privately owned anxious dogs

The objectives of this study were to evaluate the effects of a diet (CALM CANINE) supplemented with alpha-casozepine and L-tryptophan on the stress response of anxious dogs with a focus on anxiety-related behavioral parameters and physiological parameters. Forty-four privately owned dogs were first fed with the control diet, followed by the study diet, each for an 8-week period, with a transitional period of 1 week between the diets. After 7 weeks on a particular diet, owners reported their dogs' behavior by filling out a questionnaire. Using the Canine Behavioral Assessment and Research Questionnaire, the effect of the study diet was found to be significant for 4 anxiety-related behavioral parameters, possibly mirroring a placebo effect. After 7 weeks on each diet, the urine cortisol-to-creatinine ratio (UCCR) was measured to assess the stress response to a visit to a veterinary practice for toenail clipping. UCCR was measured to complement the interpretation of behavioral data with regard to the stress susceptibility of the dogs. A positive correlation was found between baseline UCCR, as measured in the home situations, and poststressor UCCR. The stressor-induced increase was significantly lower in the dogs when they were fed the study diet than earlier when they were fed the control diet. The supplementation of casozepine or the proportion of protein in a given diet could have been a determining factor. The study diet seems to improve the ability of an individual to cope with stress and may reduce anxiety-related behavior in anxious dogs.     

A novel method for lifting weanling research pigs

It is possible to modify lifting techniques in small laboratory pigs to evoke less of a fear response, strengthen the human–animal bond, and improve welfare. The authors hypothesized that recently weaned pigs lifted with a ventral (belly) scoop method would show less fear of new humans and less fear during treatment than pigs lifted vertically by their hind limbs. To evaluate this hypothesis, 32 Yorkshire-cross pigs (age 3 weeks) were divided into 8 groups of 4. All pigs were acclimated to humans for 11 days and uniformly enriched prior to any lifting tests. Pigs were randomly assigned to 1 of 4 different combinations of ventral and/or vertical lifting techniques that varied by consecutive day (1 ventral-ventral; 2 ventral-vertical; 3 vertical-ventral; 4 vertical-vertical). Each day, data were collected regarding pigs’ aversion to lifting and willingness to be caught multiple times. Two hours later, their time to approach a new person and proportion of time spent hiding from the new person were also measured. Aversion scores were assigned based on duration of squealing, shaking, and freezing responses during lifting. Pigs that were lifted using alternate methods on consecutive days showed significantly less aversion when being scooped ventrally (P = 0.008) as compared to lifted vertically by their hind limbs. Additionally, behavioral trends were identified during the study. Based on standardized aversion scores, this research shows that the ventral scoop method induces less fear than the vertical lift method.     

Comparison of behavioral characteristics of dogs in the United States and Japan

This study examined the difference in dog owning between Japan and the United States, and the effect of these differences on dogs’ behavioral characteristics. Behavioral evaluations of privately-owned dogs were obtained by using online questionnaire. We compared background and demographic information from the two countries and analyzed the effects of these differences on behavioral characteristics in dogs. The results indicated that there was a bias in the dog breeds kept in Japan compared to the United States and that Japanese dogs’ body weight was lower than the US dogs. The main source of dog acquisition was pet stores in Japan and breeders and/or shelters in the United States. Multiple linear regression analysis found that Japanese dogs showed more aggression to household members and higher energy, restlessness and fear of non-social stimuli than US dogs, while US dogs showed more fear of unfamiliar persons, separation-related behavior and excitability. US dogs also showed higher levels of trainability and attachment to owners. The lower dog’s body weight was, the higher the behavioral scores except for trainability were. When dogs that were obtained under 3 months of age were analyzed, the younger the dogs were when their owners obtained them, the higher the scores on some behavioral problem factors were. The higher rates of problem behaviors among Japanese dogs compared with US dogs suggest that the preference for small breed dogs and poor early development environment influenced the behavioral characteristics of dogs.     

Long-term risks and benefits of early-age gonadectomy in dogs

OBJECTIVE: To evaluate the long-term risks and benefits of early-age gonadectomy, compared with traditional-age gonadectomy, among dogs adopted from a large animal shelter. DESIGN: Retrospective cohort study. ANIMALS: 1,842 dogs. PROCEDURE: Dogs underwent gonadectomy and were adopted from an animal shelter before 1 year of age; follow-up was available for as long as 11 years after surgery. Adopters completed a questionnaire about their dogs' behavior and medical history. When possible, the dogs' veterinary records were reviewed. Associations between the occurrence of 56 medical and behavioral conditions and dogs' age at gonadectomy were evaluated. RESULTS: Among female dogs, early-age gonadectomy was associated with increased rate of cystitis and decreasing age at gonadectomy was associated with increased rate of urinary incontinence. Among male and female dogs with early-age gonadectomy, hip dysplasia, noise phobias, and sexual behaviors were increased, whereas obesity, separation anxiety, escaping behaviors, inappropriate elimination when frightened, and relinquishment for any reason were decreased. CONCLUSIONS AND CLINICAL RELEVANCE: Because early-age gonadectomy appears to offer more benefits than risks for male dogs, animal shelters can safely gonadectomize male dogs at a young age and veterinary practitioners should consider recommending routine gonadectomy for client-owned male dogs before the traditional age of 6 to 8 months. For female dogs, however, increased urinary incontinence suggests that delaying gonadectomy until at least 3 months of age may be beneficial.     

Advances in the understanding and treatment of noise phobias

Fear is a whole body response that may result in behavioral, physiologic, and emotional adaptations that function to protect the animal. Adaptive fear responses occur in the context of threat and are appropriate for the particular response requirements of the fear-provoking situation. Maladaptive fear responses occur out of context or in excess of the demands of the situation. Recent evidence implicates dysregulation of the brain noradrenergic system in the pathophysiology of fear disorders. Noise phobias in dogs meet the criteria of a phobia. Clinical and early laboratory findings suggest there may be inherent individual predispositions in dogs for the development of these disorders. It has been suggested that noise phobic dogs have a auditory sensitivity to particular sounds. Treatment of noise phobias should focus on reducing the response to the phobic sound rather than on the behaviors exhibited during the fear response.     

Fear-related behaviour of dogs in veterinary practice

To investigate further the occurrence of fear-related behaviour in dogs in veterinary practice and to evaluate associated factors, 135 dogs were observed under practice conditions within the framework of a standardised test examination and the owners interviewed using a questionnaire. Most dogs exhibited fear reactions, particularly on the examination table, with 78.5% (106/135) categorised as ‘fearful’ based on their behaviour. Unlike weight and castration, age, gender and previous experience were significantly (P < 0.05) associated with fearful behaviour. Male dogs were significantly less ‘fearful’ than females and animals under <2 years were significantly less ‘fearful’ compared with older dogs. Those with only positive previous experiences in veterinary surgeries were significantly less ‘fearful’ than dogs that had a previous negative experience. Fear-related behaviour in veterinary practice is an issue of importance.     

Efficacy of a diet containing caseinate hydrolysate on signs of stress in dogs

The purpose of this randomized, double-blind, placebo-controlled trial was to evaluate the efficacy of a diet containing caseinate hydrolysate (CH) on signs of stress in 2 groups of dogs (defined as Anxious and Nonanxious), using physiological (serum cortisol and lysozyme, N:L ratios and heart rate) and behavioral parameters.From an initial group of 40 female Beagle dogs, ranging in age from 10 months to 4 years (mean = 1.47 years; SD = 0.53) belonging to a dog colony, 32 were selected for this study according to their level of anxiety. A group of 16 Anxious dogs and a group of 16 Nonanxious dogs were identified.A baseline period, aimed to obtain reference values of investigated parameters, preceded the experimental phase. Both groups (Anxious and Nonanxious) were divided into a treatment group, which received the diet containing CH, and a control group which received a placebo diet (PD). Anxious CH and PD groups were balanced for anxiety level. Each dog was evaluated 3 times a day at 4 weeks intervals (T1-T2-T3). Each evaluation lasted 2 days and involved a Reactivity Evaluation Form, a blood sampling, heart rate recording, and a 10-minute behavioral video recording. Results from Reactivity Evaluation Form scores showed that although at T1 Anxious dogs had significantly higher scores (Mann–Whitney test, P < 0.001) compared with Nonanxious dogs, no difference was found between Anxious dogs fed with CH diet and Nonanxious fed with PD or CH diet at T3. Behavioral observations evidenced some signs of improvement in Anxious dog fed with CH diet. Cortisol level significantly decreased in Anxious dogs fed with CH diet (Friedman test, P < 0.05). Individual differences in physiological measures of stress responses may have contributed to the large variability, making interpretation of these measures difficult. These results suggest that CH may be used as a functional ingredient alleviating stress in dogs.     

Breed‐specific variation of hematologic and biochemical analytes in healthy adult Bernese Mountain dogs

Background: Hematology and serum biochemistry reference intervals in dogs may be affected by internal factors, such as breed and age, and external factors, such as the environment, diet, and lifestyle. In humans, it is well established that geographic origin and age may have an impact on reference intervals and, therefore, more specific reference intervals are sought for subpopulations. Objective: The objective of this study was to validate and transfer standard laboratory reference intervals for healthy Bernese Mountain dogs and to create new intervals for analytes where the established laboratory reference intervals were rejected. Methods: The procedure was performed using the human Clinical and Laboratory Standards Institute‐approved model modified for veterinary use. Thirty‐two dogs were included in the study using a direct a priori method, as recommended. Results: While 23 of the standard laboratory reference intervals were readily validated, 7 of the analytes (eosinophils, MCHC, alkaline phosphatase [ALP], γ‐glutamyltransferase, total bilirubin, amylase, and cholesterol) required new reference intervals according to the standard. These were calculated using the robust method. In particular, the new reference range for ALP was wide compared with the established laboratory reference interval. No clinical causes were found for differences in the results of these analytes. Conclusion: We found significant differences in 7 hematologic and serum biochemical analytes for which a breed‐specific variation appears to be the most plausible explanation. Breed‐specific reference intervals for Bernese Mountain dogs will help avoid misinterpretation of laboratory results in the diagnostic process.     

Behavior of laboratory beagles towards humans: Assessment in an encounter test and a simulation of experimental situations

Because of the close emotional bonds between the man and the domesticated dog, husbandry of laboratory dogs is regarded with particular interest by the public. The relationship between laboratory dogs and the man is particularly important for animal welfare, and daily contact with caregivers and handlers should not elicit fear in dogs. The aim of this study was to investigate the behaviors of laboratory dogs toward humans. In 4 research facilities, 90 male and female 1- to 10-year-old laboratory beagles of different origins (facility bred or from other origins) were tested in an “encounter test” and a setting that simulated realistically general research situations. No significant differences were observed in the encounter test when the dogs were approached by a familiar caretaker or by an unfamiliar person. Nearly all of the dogs could be caught easily, and they exhibited pronounced interest in and established contacts with both persons. We simulated an experimental manipulation by placing the dogs on a treatment table in an examination room and having them restrained by a familiar caretaker; this resulted in submissive behavior and/or signs of distress in many dogs (e.g., 12% urinated when being lifted onto the examination table and most dogs showed muzzle licking on the table). Multifactorial analysis of variance revealed that, based on most of the calculated scores, the facility-bred dogs were significantly less fearful than those obtained from commercial breeders or from other research facilities. Sex differences were not observed, and younger dogs were significantly more difficult to catch. The data revealed that good relationships were established between the caretakers and the dogs in all 4 research facilities. The facility-bred dogs in particular were friendly and exhibited little fear toward both the familiar and unfamiliar persons.     

Placebo-controlled double-blind clomipramine trial for the treatment of anxiety or fear in beagles during ground transport

The purpose of this explorative study was, first, to document changes in physiological parameters and behavior observed in dogs following ground transport and, second, to measure the effects on the above variables of a short-term administration of clomipramine, anecdotally already prescribed in private veterinary practice to reduce fear, anxiety, or both. Twenty-four beagles were randomly allocated to either clomipramine (2 mg/kg, q12h for 7 d) or placebo treatment, and then transported 3 times in a truck for 1 hour. Physiological parameters (cortisol, neutrophil:lymphocyte (N:L) ratio, heart rate) and behavior were recorded and analyzed. Clomipramine significantly reduced plasma cortisol (P < 0.05) following transport and tended (P = 0.07) to reduce N:L ratio. Clomipramine tended to only reduce "moving and panting" and drooling. Short-term administration of clomipramine appears to slightly reduce fear, anxiety, or both during transport. More research is needed to confirm the efficacy of this treatment and the appropriate dosage.