An experiment on the relative importance of denitrification,nitrate reduction and ammonification in coastal marine sediment

Simultaneous determinations of ¹⁵N-nitrogen gas production, ¹⁵N-ammonia formation by nitrate reduction and ammonia production from organic nitrogen were carried out after addition of ¹⁵N-nitrate to anaerobically incubated sediment slurries from a location in the Dutch Wadden Sea. The rate of nitrogen gas production was 1.57 μmol N·cm⁻³ wet sediment·d⁻¹, nitrate reduction to ammonia occurred at a rate of 0.79 μmol N·cm⁻³·d⁻¹ and ammonification at 1.17 μmol N·cm⁻³·d⁻¹. At the end of the incubation experiment (after 30 h) 63% of the originally added ¹⁵N-nitrate was present as nitrogen gas, 32% ended up as ammonia and ∼4% as particulate organic nitrogen. Ammonia production by degradation of organic nitrogen compounds was the most important process in building up the ammonia pool in the anaerobic sediment incubation.     

A nitrogen budget of the Scheldt hydrographical basin

A nitrogen budget including nitrite, nitrate, ammonium and organic nitrogen is presented for the western Scheldt estuary.The nitrogen entering the estuarine zone is evaluated from measurements of NO₂⁻, NO₃⁻, NH₄⁺ and organic nitrogen concentration at Rupelmonde. These results are part of 10 years survey (1973–1983) of water quality in the Scheldt estuary.The origin of this load in the Scheldt estuary is further investigated by the evaluation of the contribution of domestic, industrial sewages, agriculture and breeding in the nitrogenous load of the upper Scheldt drainage basin. Domestic load is evaluated from the watershed population. Industrial sewages are quantified by use of the evaluation of specific nitrogen spoilage by the various industries as a function of their number of workers. Nitrogen leaching of agricultural soils has been measured by determining the nitrogen concentration in small river draining agricultural areas, upstream any domestic or industrial discharges. Cattle-farming wastes are for the biggest part spread on soils. A fraction however is directly rejected in rivers.Denitrification in the tributaries of the Scheldt is important in the control of nitrate entering the estuarine zone. Its evaluation will be presented.In the estuarine part of the Scheldt (Rupelmonde-Vlissingen), the nitrogenous load is important due to the upstream load and to the sewages of the Antwerp district. These sewages (domestic, industrial, agricultural) have been evaluated as described above for the upper Scheldt basin.The important load carried at that moment by the Scheldt gives rise to an important bacterial activity which results in anaerobic conditions. Denitrification then takes place. This process reduces NO₃⁻ to N₂O and N₂, i.e. eliminates a substantial fraction of the nitrate load in the Scheldt. The importance of this process will be quantified both by measurement of in situ denitrifying activities and by analysis of NO₂⁻ + NO₃⁻ profiles in the river.When reoxidation of the water occurs by reaeration and mixing with well aerated seawater, the total mineral nitrogen has a conservative behaviour as indicated by the (straight) linear relationships between Σ N_min and chlorinity, in spite of the primary production, bacterial activities and sediment influence. This conservative behaviour of Σ N_min is used in this work for evaluating N_min exportation by the Scheldt to the North Sea. The straight line relation extrapolated at low salinity gives a “fictive nitrogen concentration” in fresh water. The product of this “fictive concentration” and the upstream discharge gives an accurate evaluation of the exportation flux of mineral nitrogen to the sea.This work shows the predominant role of denitrification in tributaries of the drainage basin and in the estuary itself as a nitrogen sink which reduces the amount of nitrogen exported by the Scheldt to the North Sea.It is suggested that the pursuit of the present waste water treatment policy, only based on the elimination of the organic load without any tertiary treatment, could result in increasing the nitrogen output into the Belgian-Dutch coastal zones by a factor 2–3.     

Primary production and nitrogen assimilation in the North sea during July 1987

An extensive survey of the whole of the North Sea, carried out in July 1987, is described. The study concentrated on the measurement of surface water inorganic nutrient concentrations and concomitant rates of primary production (¹⁴C) and nitrogen assimilation (¹⁵N). Primary production was investigated using size fractionation techniques. Three vertical profiles of primary production and nitrogen assimilation were also investigated.Much of the North Sea exhibed thermal stratification. Surface nutrient concentrations were low and chlorophyll concentrations typically <1 mg m⁻³. More than 75% of the primary production was attributable to cells <5 μm in diameter. Ammonium assimilation accounted for most of the nitrogen assimilation.The water column was vertically well mixed in the coastal zones. Here, inorganic nitrogen concentrations were high (e.g. up to 25 μmol NO₃⁻ with chlorophyll concentrations up to 10 mg⁻³, and organisms >5 μm diameter accounted for most of the primary production. As in offshore regions ammonium accounted for the major part of the nitrogen assimilated.A 115 km section obtained using an undulating oceanographic recorder showed that in certain regions of the North Sea physical features acted to increase the dependence of the phytoplankton on nitrate.     

Comparison of the pigmentation of two strains of the prymnesiophyte Phaeocystis sp.

Two strains of Phaeocystis sp., one isolated from the Weddell Sea region (Antarctica) and one from the North Sea, were compared for their growth characteristics and pigmentation during growth in batch cultures. Experiments were performed starting with identical nutrient and light conditions at 2°C, 7°C and 10°C. Division rates ranged from 0.17 to 0.94 d⁻¹ depending on strain and temperature: the Antarctic strain grew fastest at 2°C (μ=0.71 d⁻¹), the strain from the North Sea at 10°C (μ=0.94 d⁻¹). Growth phase, phase in the diurnal cycle and temperature influenced the 19'hexanoyloxyfucoxanthin to chlorophyll a ratio in both strains. Large differences in this ratio were found between flagellates and colony cells from the same strain. Despite variability within each strain, mean levels of 19'hexanoyloxyfucoxanthin were always higher in the Antarctic strain. Another fucoxanthin-related pigment, 19'butanoyloxyfucoxanthin, showed the same trends during growth as 19'hexanoyloxyfucoxanthin in the antarctic strain but was undetectable in the strain isolated from the North Sea. A comparison was made with field data collected during the Phaeocystis blooming period in the coastal zone of the North Sea. During this period relative amounts of 19'hexanoyloxyfucoanthin as well as the absence of 19'butanoyloxyfucoxanthin matched with the results from the North Sea strain in culture. Environmental factors influence pigment content and ratio, yet the two investigated Phaeocystis strains can be distinguished on the basis of their pigment characteristics.     

Soil compaction and water table effects on soil aeration and corn growth in a greenhouse study

A greenhouse experiment was conducted to study the effects of aeration on growth of corn seedlings. Variable aeration regimes were created by a combination of 3 levels of soil compaction (1.20, 1.37 and 1.56 Mg m⁻³) and 3 water table depths (10-cm, 20-cm and 30-cm). Soil aeration was assessed by measuring the oxygen diffusion rate (ODR) and the gaseous composition of soil air. Samples of soil air were analyzed for concentrations of oxygen, carbon dioxide, ethylene and nitrous oxide.The ODR decreased with increasing soil compaction. The average ODR for the 30 cm water table was 193.5, 89.2 and 15.4 μg O₂ m⁻² s⁻¹ for the three compaction treatments of 1.2, 1.37 and 1.56 Mg m⁻³, respectively. At 40 days after planting, concentration of carbon dioxide in soil air was as high as 0.5% by volume. High concentrations of ethylene and nitrous oxide were also measured in those samples that contained high levels of carbon dioxide. The range of concentration was 2–19 ppm for ethylene and 2–20 ppm for nitrous oxide.The vegetative growth of corn seedlings, as measured by root and shoot weights and plant height, was not significantly affected by the treatments imposed. However, stomatal conductance and photosynthesis significantly differed among treatments.     

The new nitrification inhibitor 3,4-dimethylpyrazole succinic (DMPSA) as an alternative to DMPP for reducing N2O emissions from wheat crops under humid Mediterranean conditions

Nowadays agricultural practices are based in the use of N fertilizers which can lead to environmental N losses. These losses can occur as nitrous oxide (N₂O) emissions as result of the microbial processes of nitrification and denitrification. N₂O together with carbon dioxide (CO₂) and methane (CH₄) are the strongest greenhouse gases (GHG) associated with agricultural soils. Nitrification inhibitors (NI) have been developed with the aim of decreasing fertilizer-induced N losses and increasing N efficiency. One of the most popular NI is the 3,4-dimethylpyrazol phosphate (DMPP) which have proven to be an advisable strategy to mitigate GHG emissions while maintaining crops yield. A new NI, 3,4-dimethylpyrazole succinic (DMPSA), has been developed. The objective of this study was to compare the impact of the new nitrification inhibitor DMPSA on greenhouse gases emissions, wheat yield and grain protein with respect to DMPP. For this purpose a field-experiment was carried out for two years. Fertilizer dose, with and without NIs, was 180 kg N ha⁻¹ applied as ammonium sulphate (AS) split in two applications of 60 kg N ha⁻¹ and 120 kg N ha⁻¹, respectively. A single application of 180 kg N ha⁻¹ of AS with NIs was also made. An unfertilized treatment was also included. The new nitrification inhibitor DMPSA reduces N₂O emissions up to levels of the unfertilized control treatment maintaining the yield and its components. The DMPSA shows the same behavior as DMPP in relation to N₂O fluxes, as well as wheat yield and quality. In spite of applying a double dose of N at stem elongation than at tillering, N₂O losses from that period are lower than at tillering as a consequence of the influence of soil water content and temperature reducing the N₂O/N₂ ratio by denitrification. NI efficiency in reducing N₂O losses is determined by the magnitude of the losses from the AS treatment.     

The recovery of benthos following the impact of low oxygen content in the German Bight

The oxygen deficiency in the German Bight and in Danish waters 1981–1983 with ensuing benthos mortality was the reason for studying the development and re-establishment of the macrofauna in the following years. These years, from 1984–1987, exhibited more favourable oxygen conditions.The macrofauna of this region with its predominantly sandy substrate belongs to the Tellina fabula community. It is dominated by regular seasonal and ephemeral species such as Spio filicornis, Phoronis spec., Spiophanes bombyx and Lanice conchilega.In 1983, a year with exceptionally low oxygen content in bottom waters (< 1 mg O₂ dm⁻³), the macrofauna showed, in some parts of the investigated area, as reduction of approx. 30–50% in species numbers. Concurrently, the individual numbers of the remaining species were reduced. Beginning in 1984, a rapid recovery of benthic communities was observed. By 1986, biomass as well as species- and individual numbers rose to values similar to those determined by other authors in earlier surveys of the German Bight. In particular, juvenile Echinocardium cordatum and crustaceans have been observed since 1984 in larger numbers.     

Actual flux of the Huanghe (yellow river) sediment to the Western Pacific ocean

More than 90% of the Huanghe sediment load is deposited in the lower reaches of the river and within the shallow estuarine area. Additional sedimentation occurs in the southern part of Bohai so that the actual flux of sediment to the Yellow Sea (Huanghai) is very limited. We show that this flux takes place via a nepheloid layer across the Bohai Sea strait, transporting ∼ 6·10⁹ kg·y⁻¹, i.e. less than 1% of the Huanghe sediment discharge.     

The role of small,low density particles on the partition of selected PCB congeners between water and suspended matter (North Sea area)

The dissolved and particulate suspended concentrations of five PCB congeners (with 3–7 chlorine atoms) in the Southern Bight (North Sea) are interpreted in terms of an equilibrium partitioning model. K_d values for riverine/coastal samples generally increase with chlorine number of the congeners (K_d = 10⁴–10⁶). Values are more than an order of magnitude larger at the low suspended matter concentrations offshore (< 1 mg·dm⁻³). This is interpreted in terms of the presence of different SPM fractions with different contents of PCB: a fraction consisting of low size/density particles carrying high contents of PCBs, dominates at low SPM concentrations. The contribution of particulate forms to the total PCB concentration per unit volume may, therefore, be significant even at low SPM concentrations.     

Community structure and biomass distribution of seagrasses and macrofauna in the flores sea,Indonesia

In several locations in the Flores Sea region the community structure and the biomass distribution of seagrasses were studied along transects perpendicular to the shoreline. The share of each species within a sample plot was estimated, divided in above- and below-ground biomass. Statistics regarding substrate coverage, shoot density and leaf-area index were sampled. A standard relation was calculated between seagrass dry weight, ash-free dry weight and organic carbon content.The biotic data were related to environmental factors: DOC and nutrients in the water, salinity, tidal amplitude, sediment composition. A relation was estimated between bottom coverage of seagrasses and standing stock. Further calculations of biomass-production ratios allow a quick and rough estimate of seagrass productivity. Maximum above-ground biomass values (500–700 g AFDW·m⁻²) together with qualitative data indicate resource (= space) partitioning among the component seagrasses within a community, and suggest a carrying capacity of the reefflat habitat for seagrass density and biomass.A tentative model was constructed, starting from a constant, non-distributed multispecies vegetation in the lower intertidal and subtidal zone on sand and coral rubble, and moving into several suboptimal situations. The upper shore carries an impoverished, constrained vegetation (irregular tides, desiccation, harvesting). Sediment reworking by animals and physical displacement of sand disturbs the vegetation and favours pioneer species. Muddy habitats bordering mangroves carry monospecific stands showing extremely high biomass (e.g. below-ground Enhalus acoroides 3500 g AFDW·m⁻²). Thalassia hemprichii and Enhalus acoroides are the most constant species in all habitats mentioned.Macrofauna biomass within the seagrass beds fluctuated widely (maximum values 50–70 g AFDW·m⁻² in mixed seagrass vegetations) and only a weak relation between benthic macrofauna biomass and seagrass community structure and biomass was found.     

Settlement,abundance, growth and mortality of juvenile flatfish in a subtropical tidal estuary (Georgia,U.S.A.)

The population dynamics of juveniles of some flatfish species were studied in the Duplin River, a tidal creek in a subtropical salt-marsh area in Georgia, U.S.A. from April until September 1990. Seven species were found. Paralichthys dentatus, Paralichthys lethostigma, Paralichthys oblongus and Trinectus maculatus were relatively rare. Etropus crossotus, Citharichthys spilopterus and Symphurus plagiusa were abundant and settled during the period studied. E. crossotus was the most abundant species with a mean abundance of 18 ind·10⁻² m⁻² (max. 287). Demersal settlement of E. crossotus took place in shallow areas and over sandy bottoms from mid-May to August. Prolonged settlement hampered the calculation of growth rate and instantaneous mortality rate. However, laboratory growth experiments indicated a mean growth of about 0.50 mm·d⁻¹ at 24–28°C. Juveniles of C. spilopterus were already present in the Duplin River in March. Settling continued until the end of April with a mean abundance of 3.5 ind·10⁻² m⁻² (max. 183). With increasing size the juveniles of this species tended to migrate to deeper waters and to the mouth of the river, possibly as a reaction to increasing water temperatures. Maximum growth rate was 1.4 mm·d⁻¹ at about 26°C. The mean instantaneous mortality rate (z) was estimated at 0.03·d⁻¹. Settling of S. plagiusa occurred from mid-May onwards. The mean abundance was 10.3 ind·10⁻² m⁻² (max. 98.3). Newly settled juveniles were most abundant on muddy sediments in the shallow river areas. The maximum growth rate was 1.3 mm·d⁻¹ at about 28°C. The mean instantaneous mortality rate (Z) decreased from 0.04·d⁻¹ in April to 0.01·d⁻¹ in August. At all sites the abundance of juveniles of this species decreased with increasing water depth. Predation experiments indicated that blue crabs (Callinectes similis and C. sapidus) and sea robins (Prionotus sp.) are potential predators on juvenile flatfish. The high abundances of juvenile flatfish indicate that the tidal creeks are an important nursery area. The correspondence between growth rates estimated from field data and those observed in the laboratory suggests that growth in the nursery is mainly related to water temperature and not food limited.     

Recent sediment deposition rates in the oyster ground,North Sea

Recent sediment accumulation rates were calculated from downcore ²¹⁰Pb-excess activity profiles obtained from box cores taken in the Oyster Ground, North Sea. The rates ranged from 0.04 to 0.94 cm·yr⁻¹ with an average of 0.39 cm·yr⁻¹. In the top sediment layer the activities were rather low, the highest being only 1.15 dpm·g⁻¹, and the average value 0.92 dpm·g⁻¹.As shown by X-ray radiographs, downcore variations in texture were small: most samples being homogeneous and lacking primary sedimentary structures. Burrowing was evident in all cores, and most of them had a surface mixed layer of at least 5 cm, below which the ²¹⁰Pb activity rapidly dropped to background value.Downcore deviations from the logarithmic decrease in ²¹⁰Pb with depth could generally be attributed to biological disturbance or physical processes causing reworking of the sediment. Only in one core did the ²¹⁰Pb activity decrease regularly with depth. The activities of ¹³⁷Cs in this core and another demonstrated recent accumulation rates of 0.44 and 0.88 cm·yr⁻¹, respectively, which are in good agreement with the rates obtained from the ²¹⁰Pb method in the same cores (viz. 0.37 and 0.94 cm·yr⁻¹). From these sedimentation rates the total amount of mud yearly deposited in the muddy part of the Oyster Ground is estimated to be in the order of 2×10⁹ kg·yr⁻¹, which is 4 to 5% of the total amount of mud yearly deposited in the North Sea.     

Measuring the flux of oxygen to a muddy sediment with a cylindrical microcosm

We have examined the effects of changing the flow velocity and the oxygen concentratin in the water overlying a muddy sediment on the flux of oxygen across the sediment-water interface and on the distribution of oxygen within the pore water. The experiment was carried out on an intertidal sediment from the western Wadden Sea, using a cylindrical microcosm with a calibrated flow regime. Steady-state and transient-state models were used to estimate the values of the effective diffusion coefficient for oxygen in the pore water.Increasing the flow velocity caused a significant though small increase in the oxygen concentration in the pore water, but had little effect on the concentration gradient at the sediment-water interface. The concentration gradient in the boundary layer was too small at any of the flow velocities to account for the oxygen flux into the sediment via molecular diffusion. This is ascribed to a pressure gradient which exists in rotating flows, disrupting the diffusive boundary layer and augmenting the flux via advection. Model calculations indicate that about 25% of the flux can be attributed to irrigation by burrowing organisms, but in contrast to previous results with sandy sediments, irrigation of the pore water caused by the radial pressure gradient can be considered to be negligible. The effective diffusion coefficient ranged from 4·10⁻⁹ m²·s⁻¹ at a depth of 1 mm below the sediment-water interface to 1·10⁻⁹ m²·s⁻¹ deeper in the sediment. These estimates are within a factor of 1 to 3 of the modelular diffusion coefficient for oxygen, which is suprisingly close in view of the high numerical densities of meiofauna and macrofauna in this sediment.     

Structure and energy demand of the benthic soft-bottom communities in the Java Sea and around the islands of Madura and Bali,Indonesia

In July 1984 benthic research was carried out in the Java Sea and around the islands of Madura and Bali.The aim was to describe the structure of the benthic communities in terms of numerical density (numbers of dominant macrofaunal groups and meiofauna per square metre) and biomass (g ashfree dry weight (AFDW) per square metre), and in relation to geographical position and main ambient factors, i.e. water depth, bottom temperature and sediment characteristics.In addition attempts were made to estimate the activity of these benthic ecosystems, and to show their relation to the pelagic.Box-core samples, collected along west-east axis in the Java Sea and in the Strait of Madura, showed relatively poor benthic communities. The average densities of macrofaunal organisms (> 1 mm) did not exceed 250 specimens·m⁻²; the total biomass was below 1 g AFDW·m⁻²; small polychaetes and crustaceans were the dominant groups.A number of larger organisms have developed very remarkable adaptations to the muddy environment. Meiofaunal organisms (nematodes being the dominant group) numbered 0.06·10⁶ to 0.46·10⁶ specimens·m⁻². In general there is a trend from west to east towards somewhat richer communities. The actual carbon demand of the benthic ecosystem in the area investigated is tentatively estimated at an average of 38 g C·m⁻²·y⁻¹, which amounts to about 40% of the primary production.Both the very soft fluid-mud bottoms in the central Java Sea and in Strait Madura, and the relatively low amount of energy available for growth, probably prevent the establishment of well developed benthic communities.     

Temporal and lateral fluctuations in production and biomass of bacterioplankton in the western Dutch Wadden Sea

To contribute to the validation of a recently developed ecosystem model of the western Wadden Sea (EON, 1988), data on bacterial biomass and production were acquired. Seven field stations, spread over the two main basins of the estuarine system, were sampled monthly in 1986. Between these basins significant differences were found in counts, biovolume, biomass and production of bacteria (measured by the ³H-thymidine method) with consistently higher mean values of bacterial variables in the Vlie basin. Bacterial production rates of 2 to 175 mg C· m⁻³· d⁻¹ were obtained for the Vlie basin, with an annual production of 10 to 11 g C· m⁻³, while the production in the Marsdiep basin did not exceed 45 mg C·m⁻³ ·d⁻¹, with an annual production of g C·m⁻³. Bacterial biomass varied over the year from 2 to 140 mg C·m⁻³ in the study area, with a mean biomass of 39 mg C·m⁻³ in the Vlie basin and 23 mg C·m⁻³ in the Marsdiep basin. Blooms of bacteria occurred in May and July–August. Spatial and temporal fluctuations in bacterial variables are discussed, taking into account different environmental factors and the availability of food for bacteria in relation to transport and exchange of water masses between the two basins and the North Sea. Results are compared with the results as simulated by the ecosystem model.     

Effects of different manuring systems with and without biogas digestion on soil mineral nitrogen content and on gaseous nitrogen losses (ammonia,nitrous oxides)

Nitrogen (N) is the most susceptible nutrient to transformations affecting plant availability. These transformations include mineralization, immobilization, nitrification and denitrification, as well as leaching and ammonia volatilization. Use of stable wastes and other residues for biogas digestion may reduce N-losses. It is the purpose of this paper (i) to assess the effects of biogas digestion on soil mineral N (SMN) content in spring and autumn, (ii) to compare NH₃ volatilization following superficial application of different manures to a cereal crop, (iii) to compare greenhouse gas emissions of differently treated liquid slurry after its application via injection into closed slots, and (iv) to compare greenhouse gas emissions of differing manuring treatments within a whole organic stockless cropping system. The SMN content in autumn was not influenced by digestion of slurry. However, if cover crops and crop residues were harvested for digestion instead of leaving it on the field, a strong decrease of the SMN content was measured. Ammonia volatilization after application from digested slurry was higher than the volatilization from undigested slurry, likely due to the effect of the higher ammonia content and higher pH. Organic manuring by application of liquid effluents of the biogas digester, by incorporation of green manures with a narrow C/N ratio or by mulching aboveground biomass of a clover/grass-ley, resulted in a strong increase in N₂O emissions. The balance showed a 38% decrease in N₂O emissions for a whole arable organic stockless cropping system when crop residues and the clover/grass-ley were harvested, digested, and the effluents were reallocated within the same cropping system, in comparison to mulching and incorporation of the biomass as green manure. Injection of liquid cattle slurry resulted in a strong increase of N₂O emissions. The results provide some evidence that denitrification during nitrification was the driving force for the measured emission peaks. It was concluded, that biogas digestion of field residues resulted in a win-win situation, with additional energy yields, a lower nitrate leaching risk and lower nitrous oxide emissions. However, the propensity to ammonia volatilization was higher in digested manures.     

Cyclic organochlorines in the hermit crabs Pagurus bernhardus and P. pubescens from the North Sea. A comparison between winter and early summer situation

The body burdens of 5 cyclic organochlorine compounds were determined in abdomens of Pagurus bernhardus and P. pubescens from the North Sea during two seasons. The seasonal bioaccumulation of ΣPCB (as the sum of concentrations of 24 individual components) is influenced by the substances associated with food originated from the spring plankton bloom and the resuspended sediments during the winter.The patterns of individual PCB congeners depended on the weight of the abdomens, and the geographical position of sampling. In offshore samples, the findings indicated a relationship between the weight of the abdomen (roughly the age) and the bioconcentration of higher chlorinated biphenyls. The results from the coastal areas demonstrated that the uptake by food determines the steady state concentration of the higher chlorinated congeners in the hermit crab. Pagurus pubescens probably possesses a mixed-function oxygenase (MFO)-system different from that of Pagurus bernhardus because the congeners 2,2′, 3,3′, 4,5,6′-heptachlorobiphenyl (174) and 2,2′, 3,3′, 4′, 5,6-heptachlorobiphenyl (177) were absent in all samples of the former species but were always present in the latter.The geographical differences between concentrations of ΣPCB reflect the general mean-routes of water and suspended particular matter transport through the North Sea: the Southern Bight and the continental coasts were more polluted with PCBs than the central North Sea. Maximum concentrations of p-p′-DDE were found in the southern parts and parts of the central North Sea. Samples from the German Bight showed the highest concentrations of Lindane. The results indicate that the atmospheric transport of lower chlorinated biphenyls, HCB, α-HCH and Lindane may be important in the distribution of these components, because they only showed slight gradients or even none at all in the area investigated.     

Production and consumption of tropical seagrass fields in Eastern Indonesia measured with bell jars and microelectrodes

During the Indonesian-Dutch Snellius-II Expedition the production and consumption of tropical seagrass species were measured with bell jars at four sampling stations in eastern Indonesia. Applying a conversion factor of 0.29, the amount of carbon fixed and mineralized was calculated from the recorded oxygen evolution. The gross production of the different seagrass communities was found to be between 1230 and 4700 mg C·m⁻²·d⁻¹. The consumption lay between 860 and 3860 mg C·m⁻²·d⁻¹. From these data a relatively low net production of 60 to 1060 mg C·m⁻²·⁻¹ could be calculated. At one sampling station incubations were carried out at different depths in a sloping Halodule uninervis (Forssk.) Aschers meadow, which indicated that seagrasses above a depth of about 2 m may become subject to photoinhibition. A linear correlation between biomass and measured production was found for Thalassia hemprichii (Ehrenb.) Aschers. Above a biomass of 100 g DW·m⁻² the production per unit of biomass decreased due to self shading.Using microelectrodes the oxygen production of epiphytes was found to be 230 mg C·m⁻² leaf surface·d⁻¹. at 1900 μE·m⁻²·s⁻¹ assuming an epiphyte coverage of 40%. This indicated that up to 36% of the primary production in a seagrass community may be attributed to epiphytes.The seagrass fields in Indonesia were found to be healthy ecosystems with a high primary production, but organisms within the communities use the abundance of organic matter very efficiently, creating net production rates of 100 to 300 mg C·m⁻²·d⁻¹ which are similar to barren areas.