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《四川畜牧兽医》2015,(4):5
提供成套家畜人工授精器材猪:假母猪台,防滑垫,17℃恒温冰箱,数显恒温干燥箱,数显恒温水浴锅(2孔、4孔),蒸馏水机,恒温磁力搅拌器,精子密度测定仪,精虫计数板,电子精密台秤,精液运输箱,温度计,采精保温杯,干湿计,消毒棉花纱布,烧杯,量杯,量筒,试管,瓷盘,洗涤瓶,试管架,采精手套,精液过滤纸,一次性和多次性输精管,40-80ml输精瓶,润滑剂,一次性防护服,一次性脚罩,怀孕测定仪,工作水靴,精液稀释剂等。牛:颗粒输精器,卡苏枪,外管套,细管剪,一次性长臂手套,乳胶手套,液氮容器(运输罐、存罐各种规格),开膣器,内窥镜,全套实验设备器材(见猪人工授精器材)。羊:假阴道(外壳、内胎、气嘴、孔塞),输精器,开膣器,集精瓶,采精手套,50-100℃水温计,医用凡士林,全套实 相似文献
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喂饮及时,定时定量,先粗后精,配合多样。饲料更换,应要逐渐,看槽喂养,少给勤添。草饱料足,要靠时间,尤其饮水,上嚼之前。饲料调剂,品种齐全,搭配合理,营养全面。青刘青贮,长年不断,动物蛋白,适量补添。专人管理,细心钻研,因牛制宜,照料周全。人牛亲和,不打不撵,耐心调教,养成习惯。刷拭牛体,血派循环,加强运动,神旺体健。草净水净,圈要勤垫,畜舍卫生,保持一贯。夏要防暑,冬要防寒,长年采精,注意安全。异常变化,留心察看,不可疏忽,防患未然。杜绝事故,安全生产,守住现场,粗活细干。操作规程,看来简单,要想做好,并非空谈。事业之心,责任之感,… 相似文献
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南倚太行,沃野千里,草食家畜,利国富民,农林牧渔,调整结构,学张牛子,促现代化,基地建设,体系配套,肉牛育肥,黑黄并举,小尾寒羊,选种选配,大搞青贮,北跨燕山,盛产粮棉。牛羊当先,宏图再展。综合开发;良性循环。愚公移山,商品生产。项目专款,首保华幸。质量当先,先易后难。扬长避短,肥羔生产。重在普及;秸杆氨化,由点到面。棉籽饼壳,上千万担;精粗搭配,物美价廉。专业大户,成百上千,均衡供应,市场优选。疫病防治,层层把关;综合服务,推行保险。方向明确,贵在落实,系统工程,扣紧连环。三日.论证,求计间贤,横向联合,任重道远。 李易方 1986年6月2… 相似文献
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牛发情,频排尿,看阴道,口略张,随时间,中期粘,摸卵巢,摸其状,此期中,泡素长,卵壁厚,至末期,泡壁薄,卵泡破,症状明,腰背拱,色潮红,光泽明。有变动,牵缕性,有活动,豆粒形,不输精。泡液生,有弹性,泡液增,波动明,泡壁松,常阵叫,爱爬跨,子宫颈,分泌物,初时稀,末期稠,初发情,无弹性,至中期,卵泡体,此期中,泡体园,似樱桃,空腔软,神不宁。阴门肿。花蕾形,色透明;似卵清;呈浓性。泡小硬,无波动;卵泡中,小球形;可输精。鼓棱棱,烂熟性。呈凹形; 此期中,必输精。排卵后,黄体生, 摸其状,面团形,遇此景,配酌情。 发情牛,形不同,看膘情,分年令,·“少”配晚… 相似文献
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Valorie S. Mackey DVM Donald R. Trout DVM‡ Dennis M. Meagher DVM PhD† William J. Hornof DVM MS‡ 《Veterinary radiology & ultrasound》1987,28(1):26-31
The medical records, radiographic and nuclear scintigraphic findings of 26 racing horses with 27 stress fracture episodes of the humerus, radius, or tibia were reviewed. The purposes of this study were to describe the radiographic and/or scintigraphic features of stress fractures of the humerus, radius, or tibia, and to evaluate the signalment and history of horses in which stress fracture occurred. Stress fractures of the three long bones examined were primarily seen in 2-and 3-year-old male Thoroughbred horses; commonly, the onset of lameness was immediately following training gallops or racing. There were 13 humeral stress fracture episodes in 12 horses. Ten were in the proximal caudolateral cortex, and three were in the distal craniomedial cortex. Radial stress fractures were seen in three horses, all in the midshaft radius. Tibial stress fractures were diagnosed in 11 horses. They were located in the proximal lateral tibia in six horses, the distal caudolateral tibia in three horses, and the midshaft tibia in three horses. Fifteen stress fractures were diagnosed with radiographs alone, one was diagnosed with scintigraphy alone, nine were diagnosed with radiographs and scintigraphy, and, in two horses, radiographs were negative, but the scintigraphic findings were consistent with stress fracture. 相似文献
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家兔亚硒酸中毒的病理形态学研究 总被引:1,自引:0,他引:1
给家兔分别肌肉注射不同剂量的亚硒酸钠,历时入周,复制出亚急性硒中毒的动物模型。病理形态学变化为:家兔的生长受到明显抑制,肝,肾,心,脾等组织器官损害严重如肝实质细胞变性,坏死;肾实质退行病变;心肌纤维变性,充血,肺淤血,水肿:脾萎缩,淋巴细胞坏死,肝,肾,心细胞内线粒高度肿胀,嵴减少,断裂或消失成空泡。 相似文献
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羔羊小肠pH及主要消化酶发育规律的研究 总被引:13,自引:2,他引:13
对小尾寒羊羔羊小肠不同部位(十二指肠、空肠和回肠)pH及淀粉酶、胰蛋白酶、糜蛋白酶、脂肪酶和乳糖酶的活性进行了测定,结果表明:羔羊小肠不同部位内容物pH不同,回肠pH显著高于空肠,空肠和回肠pH显著高于十二指肠,羔羊1月龄后,小肠各段pH变化不大;小肠不同部位淀粉酶和胰蛋白酶、糜蛋白酶及脂肪酶活性不同,空肠段淀粉酶、胰蛋白酶、糜蛋白酶及脂肪酶活性高于十二指肠和回肠段,随着羔羊日龄的增长,小肠淀粉酶、胰蛋白酶、糜蛋白酶及脂肪酶活性增加,3月龄后各消化酶活性变化差异不显著(P<O.05);羔羊小肠不同部位乳糖酶活性不同,空肠乳糖酶活性显著高于十二指肠和回肠,随着羔羊日龄的增长,小肠乳糖酶活性逐渐降低。 相似文献
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为了鉴定和分析五指山猪背部和腹部皮下脂肪组织中差异表达的长链非编码RNA(long noncoding RNA, lncRNA)和信使RNA(messenger RNA, mRNA),采用RNA-Seq和生物信息学方法对五指山猪皮下脂肪组织中的lncRNA和mRNA进行分析筛选,运用DESeq鉴定背部和腹部皮下脂肪组织中差异表达的lncRNA和mRNA,并对差异表达的lncRNA进行靶基因预测分析。结果显示:在五指山猪皮下脂肪组织中共鉴定出12 875个lncRNA,其中正义型10 155个、反义型278个、内含子型246个、基因间型2 196个;在背部与腹部皮下脂肪间,存在184个差异表达的mRNA,其中前十位分别是ZIC1、ZIC4、HAND2、CCBE1、RPH3A、ISM1、ANXA8、SLITRK4、DSG2、EVPL,存在45个差异表达的lncRNA,其中6个只在背部皮下脂肪中表达、18个只在腹部皮下脂肪中表达;获得差异表达lncRNA的靶基因共109个,包括顺式作用的靶基因和反式作用的靶基因。本试验为进一步研究lncRNA和mRNA调控猪皮下脂肪发育的分子机制提供科学依据。 相似文献
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上市肉鸡组织样品中铅、镉、铜、铬含量的测定 总被引:4,自引:1,他引:4
从江苏省某市采集上市肉鸡组织样品 ,采用微波消解 石墨炉 原子吸收分光光度法 ,测定主要组织 (心脏、肝脏、肌胃、胸肌、肾脏、腿肌、骨骼 )中铅、镉、铜、铬等重金属元素的含量。检验结果 :组织中Pb的含量以心脏最高 (1 3478± 0 531 2 )mg/kg ,肝脏次之 (1 1 2 6 3±0 534 0 )mg/kg ,骨骼最低 (0 1 0 1 8± 0 0 0 6 6)mg/kg ,样品含Pb超标率按上述组织顺序分别为 :1 0 0 %、 96 87%、 96 87%、 87 50 %、1 0 0 %、 81 2 5 %和 0 ;组织中Cd的含量以肾脏最高 (0 0 63 0± 0 0 2 0 2 )mg/kg,骨骼次之 (0 0 55 9± 0 0 2 0 5)mg/kg ,样品含Cd超标率分别为 :6 2 5 %、 0、 3 1 3 %、 0、 0、 0和 0 ;组织中Cu的含量以心脏最高 (8 492 7± 1 90 1 2 )mg/kg,肝脏次之 (8 462 8± 1 666 4)mg/kg ,腿肌最低 (0 8373± 0 741 4 )mg/kg ,样品含Cu超标率分别为 :1 2 50 %、 1 5 63 %、 9 38%、 3 1 3 %、 0、 0和 0 ;组织中Cr的含量以肾脏最高(1 642 5± 1 0 1 0 0 )mg/kg ,肝脏次之 (1 642 5± 0 61 97)mg/kg ,样品含Cr超标率分别为 :50 0 0 %、 62 70 %、 9 38%、 3 1 3 %、 0、 0和 0。检测数据表明 ,鸡主要组织样品中Pb含量严重超标 ;肝脏、肾脏中Cr超标较严重 ,Cd和Cu也有不同程度? 相似文献
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2009--2010年,对新疆部分地区蛋鸡、肉鸡、家养水禽的养殖场随机采集血清样品10193份,活禽市场采集血清样品1620份。用HI方法对禽流感免疫抗体进行检测,结果表明:蛋鸡的H5免疫抗体合格率为33.33%~100%,其中0效价率为33.33%-80.00%;H9免疫抗体合格率为62.4%-100%,20%-33%蛋鸡场存在100%的0效价现象。肉鸡H5免疫抗体合格率为0%-100%,其中7.69%-69.6%肉鸡群中H5抗体0效价率为100%。水禽的H5免疫抗体合格率为0%-100%,有25%33.33%鸭群全部是0效价。活禽市场H5抗体效价≥410g2的比例为10.59%-83.57%,H9抗体效价至410醇的比例21.70%-67.14%。 相似文献
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为鉴定前期转录组测序分析结果的可靠性,并从中挖掘水牛脂肪沉积潜在环状RNA(circular RNA,circRNA),本研究对10个来源于过氧化物酶体增殖物激活受体γ(PPARG)基因的circRNA进行鉴定,根据测序获得的候选circRNA序列信息,设计对向引物进行半定量检测和普通测序以获得真实存在的circRNA,采用实时荧光定量PCR分析其在水牛不同组织和不同部位脂肪组织中的表达谱,半定量检测其在不同物种间的保守性。结果显示,10个候选circRNA中,有5个circRNA可以检测到,测序验证序列与转录组测序分析获得的序列一致,其中2个circRNA可设计出特异性的定量引物,这2个circRNA主要在脂肪组织中表达,且在不同部位脂肪组织中的表达量存在差异;另外3个circRNA在黄牛、牦牛、猪和小鼠的脂肪组织中均有表达,序列长度一致,序列相似度高。以上研究结果提示,转录组测序分析获得的结果可靠,其中有2个circRNA主要在脂肪组织中表达,且物种间保守,可作为研究水牛脂肪沉积的候选基因。 相似文献
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White nodules were observed in the thyroid in two male C3H mice (at 99 and 122 weeks of
age) exposed to fast neutrons at the age of 8 weeks. Histopathologically, in both cases,
tumors were developed in the region corresponding to the parathyroid gland, and the tumor
cells were arranged in a solid sheet or nest-like structures. Necrosis, cell debris and/or
hemorrhage were sometimes seen in the center of the tumor structures. Tumor cells were
small and uniform with scanty cytoplasm, cell margins were indistinct, and basally located
tumor cells were aligned along the vascular stroma. Mitotic figures were frequently
observed. Metastasis to the renal cortex was observed in both cases. These cases were
diagnosed as parathyroid carcinoma. A parathyroid tumor is an extremely rare endocrine
tumor in mice, regardless of whether the tumor is spontaneous or experimentally induced.
These cases may have been induced by neutron-exposure; however, how radiation induces
parathyroid carcinoma in mice is not clear. 相似文献
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Prasit Supakorndej PhD Robert E. Lewis DVM MS John W. McCall PhD Michael T. Dzimianski MS DVM Robert A. Holmes DVM PhD 《Veterinary radiology & ultrasound》1995,36(1):23-29
Five ferrets of each sex were each inoculated with 15 third-stage infective larvae of Dirofilaria immitis to study radiographic and angiographic changes in the cardiopulmonary system following heartworm infection; 5 additional ferrets of each sex served as noninfected controls. Prior to inoculation and every 8 weeks thereafter until 40 weeks, the infected and noninfected ferrets were radiographed; angiographic examinations were done prior to necropsy. At necropsy, the worms in the heart, lungs, and associated vessels were counted, and lung histosections were prepared and examined for changes. Radiographic changes were seen in the right side of the heart and associated vessels of infected ferrets as compared with the noninfected ferrets, but changes were less prominent than those seen in heartworm-infected dogs and cats. The changes were primarily an increase in the size of the right side of the heart, especially the right atrium. Radiographically, no changes could be visualized in the pulmonary vascular system. Worms in the enlarged cranial vena cava, azygous vein, and left caudal lobar pulmonary artery of infected animals were delineated by angiography. Histologically, no changes were seen in the pulmonary vascular tissues. 相似文献
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用不同剂量(10、30、50mg/kg)的小花棘豆总黄酮给小鼠灌胃,观察其对小鼠游泳能力的影响,待小鼠力竭后测定其13项血液生理指标,血液和器官组织的相关酶活性以及中间代谢产物的含量。结果表明,添加小花棘豆总黄酮可延长小鼠游泳至力竭的时间,降低机体白细胞总数和粒细胞百分数,提高血液中淋巴细胞百分数、中值细胞百分数、粒细胞数、血红蛋白浓度、红细胞压积等指标,有效提高了小鼠血清和组织中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性,降低了血清谷草转氨酶和肌酸激酶的活性;降低了脂褐素、丙二醛、乳酸和尿素氮的含量,增加了肌糖原和肝糖原的含量。说明小花棘豆总黄酮具有较好的抗氧化损伤功效。 相似文献