A re‐evaluation of the enriched labile soil organic matter fraction

Identifying ‘functional' pools of soil organic matter and understanding their response to tillage remains elusive. We have studied the effect of tillage on the enriched labile fraction, thought to derive from microbes and having an intermediate turnover time. Four soils, each under three regimes, long‐term arable use without tillage (NT), long‐term arable under conventional tillage (CT), and native vegetation (NV), were separated into four aggregate size classes. Particle size fractions of macro‐ (250–2000 μm) and microaggregates (53–250 μm) were isolated by sonication and sieving. Subsequently, densiometric and chemical analyses were made on fine‐silt‐sized (2–20 μm) particles to isolate and identify the enriched labile fraction. Across soils, the amounts of C and N in the particle size fractions were highly variable and were strongly influenced by mineralogy, specifically by the contents of Fe and Al oxides. This evidence indicates that the fractionation procedure cannot be standardized across soils. In one soil, C associated with fine‐silt‐sized particles derived from macroaggregates was 567 g C m^?2 under NV, 541 g C m^?2 under NT, and 135 g C m^?2 under CT, whereas C associated with fine‐silt‐sized particles derived from microaggregates was 552, 1018, 1302 g C m^?2 in NV, NT and CT, respectively. These and other data indicate that carbon associated with fine‐silt‐sized particles is not significantly affected by tillage. Its location is simply shifted from macroaggregates to microaggregates with increasing tillage intensity. Natural abundance ¹³C analyses indicated that the enriched labile fraction was the oldest fraction isolated from both macro‐ and microaggregates. We conclude that the enriched labile fraction is a ‘passive' pool of soil organic matter in the soil and is not derived from microbes nor sensitive to cultivation.     

Soil organic carbon in density fractions of tropical soils under forest – pasture – secondary forest land use changes

Our knowledge of effects of land use changes and soil types on the storage and stability of different soil organic carbon (SOC) fractions in the tropics is limited. We analysed the effect of land use (natural forest, pasture, secondary forest) on SOC storage (depth 0–0.1 m) in density fractions of soils developed on marine Tertiary sediments and on volcanic ashes in the humid tropics of northwest Ecuador. The origin of organic carbon stored in free light (< 1.6 g cm^?3) fractions, and in two light fractions (LF) occluded within aggregates of different stability, was determined by means of δ¹³C natural abundance. Light occluded organic matter was isolated in a first step after aggregate disruption by shaking aggregates with glass pearls (occluded I LF) and in a subsequent step by manual destruction of the most stable microaggregates that survived the first step (occluded II LF). SOC storage in LFs was greater in volcanic ash soils (7.6 ± 0.6 Mg C ha^?1) than in sedimentary soils (4.3 ± 0.3 Mg C ha^?1). The contribution of the LFs to SOC storage was greater in natural forest (19.2 ± 1.2%) and secondary forest (16.6 ± 1.0%) than in pasture soils (12.8 ± 1.0%), independent of soil parent material. The amount of SOC stored in the occluded I LF material increased with increasing silt + clay content (sedimentary soils, r = 0.73; volcanic ash soils, r = 0.58) and aggregation (sedimentary soils, r = 0.52; volcanic ash soils, r = 0.45). SOC associated with occluded I LF, had the smallest proportion of new, pasture‐derived carbon, indicating the stabilizing effect of aggregation. Fast turnover of the occluded II LF material, which was separated from highly stable microaggregates, strongly suggested that this fraction is important in the initial process of aggregate formation. No pasture‐derived carbon could be detected in any density fractions of volcanic ash soils under secondary forest, indicating fast turnover of these fractions in tropical volcanic ash soils.     

Qualitative and quantitative differences in particulate organic matter fractions in organic and conventional farming systems

To quantify functionally important differences in soil organic matter (SOM) that result from use of different farming practices, soils from 9 long-term trials comparing manure+legume-based organic, legume-based organic, and conventional farming systems were collected and particulate organic matter (POM) was fractionated to reflect its position within the soil matrix. The free, light POM (FPOM; <1.6 g cm⁻³) not occluded within aggregates and occluded POM (OPOM; <2.0 g cm⁻³) were compared to an undifferentiated POM fraction (coarse fraction, CF; >53 μm) obtained by wet sieving. Fraction C, N, and hydrolyzable N (quantified using the Illinois test (IL-N)) were determined. Organic farming systems had greater quantities of C and N in the OPOM and CF and, greater IL-N contents in all POM fractions considered. The OPOM's C:N ratio (16-19) and was least in the manure+legume-based organic, intermediate in the legume-based organic, and greatest in the conventional systems (P<0.10). Trends in OPOM C:N and IL-N abundance suggested occluded POM was most decomposed, and possibly a greater N reservoir, in the manured soils. The FPOM quality reflected the residues added to each system and its removal improved resolution of quality-based differences in POM associated with long-term management. Subdivision of POM revealed differences in its quality that were not evident using the undifferentiated CF. Quantification of hydrolysable N (IL-N) in POM did not enhance our understanding of management's affect on SOM quality. This multi-site comparison showed organic management simultaneously increased the size of the labile N reservoir and the amount of POM protected within aggregates; and that, occluded POM is more decomposed in manure+legume- than in legume-based organic systems. The characteristics of POM reveal how organic practices improve SOM and suggest the nutrient and substrate decay dynamics of organic systems may differ as a result of the N fertilization strategies they employ.     

Sources and composition of soil organic matter fractions between and within soil aggregates

It is generally accepted that particulate organic matter derives from plants. In contrast, the enriched labile fraction is thought by many to derive from microbes, especially fungi. However, no detailed chemical characterization of these fractions has been done. In this study, we wanted to assess the sources (plants or microbes; fungi or bacteria) and degree of microbial alteration of (i) three particulate organic matter fractions – namely the free light fraction (1.85 g cm^?3), the coarse (250–2000 μm) and the fine (53–250 μm) intra‐aggregate particulate organic matter fractions – and of (ii) three density fractions of fine‐silt associated carbon – namely < 2.0, 2.0–2.2 (i.e. enriched labile fraction) and > 2.2 g cm^?3– by analysing the amino sugars, by CuO oxidation analyses, and by ¹³C‐, ¹H‐ and ³¹P‐NMR analyses. Macroaggregates (250–2000 μm) were separated by wet‐sieving from a former grassland soil now under a no‐tillage arable regime. The three particulate organic matter fractions and the three density fractions were isolated from the macroaggregates by a combination of density flotation, sonication and sieving techniques. Proton NMR spectroscopy on alkaline extracts showed that the enriched labile fraction is not of microbial origin but is strongly degraded plant material that is enriched in aliphatic moieties partly bound to aromatics. In addition, the enriched labile fraction had a glucosamine content less than the whole soil, indicating that it is not enriched in carbon derived from fungi. Decreasing yields of phenolic CuO oxidation products and increasing side‐chain oxidation in the order coarse intra‐aggregate particulate organic matter < fine inter‐aggregate particulate organic matter < fine‐silt fractions indicate progressive alteration of lignin as particle size decreases. The light fraction was more decomposed than the coarse inter‐aggregate particulate organic matter, as indicated by (i) its larger ratio of acid‐to‐aldehyde of the vanillyl units released by CuO oxidation, (ii) the smaller contribution of H in carbohydrates to total extractable H as estimated by ¹H‐NMR spectroscopy, and (iii) a larger contribution of monoester P to total extractable P in the ³¹P‐NMR spectra. In conclusion, the four fractions are derived predominantly from plants, but microbial alteration increased as follows: coarse inter‐aggregate particulate organic matter < light fraction ≈ fine inter‐aggregate particulate organic matter < enriched labile fraction.     

A comparison of two methods for the isolation of free and occluded particulate organic matter

Various methods exist for the isolation of particulate organic matter (POM), one of the soil‐organic‐matter (SOM) fractions reacting most sensitive on land‐use or soil‐management changes. A combination of density separation and ultrasonic treatment allows to isolate two types of POM: (1) free POM and (2) POM occluded in soil aggregates. POM fractions are closely linked to their biochemical function for the formation and stabilization of aggregates, therefore methods using different aggregate sizes may result in different POM fractions isolated. We evaluated two physical fractionation procedures to reveal whether they yield different POM fractions with respect to amount and composition, using grassland and arable soils with sandy‐loam to sandy–clay‐loam texture and thus low macroaggregate stability. Method I used air‐dried aggregates of <2.0 mm size and a low‐energy sonication for aggregate disruption, method II used field‐moist aggregates <6.3 mm and a high‐energy–sonication procedure for aggregate disruption. POM fractions were analyzed by elemental analysis (C, N) and CPMAS ¹³C‐NMR spectroscopy. With both methods, about similar proportions of the SOM are isolated as free or occluded POM, respectively. The free‐ and occluded‐POM fractions obtained with method I are also rather similar in C and N concentration and composition as shown by ¹³C‐NMR spectroscopy. Method II isolates a free‐ and occluded‐POM fraction with significantly different C and N concentrations. NMR spectra revealed significant differences in the chemical composition of both fractions from method II, with the occluded POM having lower amounts of O‐alkyl C and higher amounts of aryl C and alkyl C than the free POM. Due to the use of larger, field‐moist aggregates with minimized sample pretreatment, two distinctly different POM fractions are isolated with method II, likely to be more closely linked to their biochemical function for the formation and stabilization of aggregates. High‐energy sonication as in method II also disrupts small microaggregates <63 µm and releases fine intraaggregate POM. This fraction seems to be a significant component of occluded POM, that allows a differentiation between free and occluded POM in sandy soils with significant microaggregation. It can be concluded, that microaggregation in arable soils with sandy texture is responsible for the storage of a more degraded occluded POM, that conversely supports the stabilization of fine microaggregates.     

Soil organic carbon and nutrient content in aggregate‐size fractions of a subtropical rice soil under variable tillage

The effects of tillage on soil organic carbon (SOC) and nutrient content of soil aggregates can vary spatially and temporally, and for different soil types and cropping systems. We assessed SOC and nutrient levels within water‐stable aggregates in ridges with no tillage (RNT) and also under conventional tillage (CT) for a subtropical rice soil in order to determine relationships between tillage, cation concentrations and soil organic matter. Surface soil (0–15 cm) was fractionated into aggregate sizes (>4.76 mm, 4.76–2.00 mm, 2.00–1.00 mm, 1.00–0.25 mm, 0.25–0.053 mm, <0.053 mm) under two tillage regimes. Tillage significantly reduced the proportion of macroaggregate fractions (>2.00 mm) and thus aggregate stability was reduced by 35% compared with RNT, indicating that tillage practices led to soil structural change for this subtropical soil. The patterns in SOC, total N, exchangeable Ca²⁺, Mg²⁺ and total exchangeable bases (TEB) were similar between tillage regimes, but concentrations were significantly higher under RNT than CT. This suggests that RNT in subtropical rice soils may be a better way to enhance soil productivity and improve soil C sequestration potential than CT. The highest SOC was in the 1.00–0.25 mm fraction (35.7 and 30.4 mg/kg for RNT and CT, respectively), while the lowest SOC was in microaggregate (<0.025 mm) and silt + clay (<0.053 mm) fractions (19.5 and 15.7 mg/kg for RNT and CT, respectively). Tillage did not influence the patterns in SOC across aggregates but did change the aggregate‐size distribution, indicating that tillage affected soil fertility primarily by changing soil structure.     

Dynamics of soil organic matter associated with particle-size fractions of water-stable aggregates

Stable macroaggregates (> 200 μm) of cultivated soils are reported in the literature to be richer in organic carbon, and in young organic carbon in particular, than microaggregates (< 200 μm). However, the nature of this additional carbon is not yet known. To determine it, we compared the composition of organic matter in stable macroaggregates with that in unstable ones. Macroaggregates 2–3 mm in diameter were separated from two silty cultivated soils from the Paris basin. They were slaked, and the primary particle composition of the resulting fractions was analysed. We used the natural abundance of ¹³C to quantify the amount of young carbon, derived from a maize crop, in the various size fractions. The stable macroaggregates were richer in total C and in young C (younger than 6 and 23 years respectively in the two soils studied) than the unstable ones. This young C comprised 50% particulate organic matter, 20% associated with silt and 30% with clay particles. We propose a schematic composition of aggregates in these soils in which stable aggregates are formed by the binding of microaggregates by additional young organic matter, predominantly plant debris. Young organic matter is preferentially incorporated and is responsible for aggregation, though it is eventually redistributed among aggregate classes through the destruction and re‐formation of the aggregates. We have developed a model to simulate this redistribution. The model shows that stable macroaggregates have a life of a few years, but that microaggregates may exist for decades. We suggest that the stabilization and de‐stabilization of macroaggregates in soils is linked to the incorporation and biodegradation of plant debris.     

Organic matter in density fractions of water-stable aggregates in silty soils: Effect of land use

The location of soil organic matter (SOM) within the soil matrix is considered a major factor determining its turnover, but quantitative information about the effects of land cover and land use on the distribution of SOM at the soil aggregate level is rare. We analyzed the effect of land cover/land use (spruce forest, grassland, wheat and maize) on the distribution of free particulate organic matter (POM) with a density <1.6 g cm⁻³ (free POM_<1.6), occluded particulate organic matter with densities <1.6 g cm⁻³ (occluded POM_<1.6) and 1.6-2.0 g cm⁻³ (occluded POM_1.6-2.0) and mineral-associated SOM (>2.0 g cm⁻³) in size classes of slaking-resistant aggregates (53-250, 250-1000, 1000-2000, >2000 μm) and in the sieve fraction <53 μm from silty soils by applying a combined aggregate size and density fractionation procedure. We also determined the turnover time of soil organic carbon (SOC) fractions at the aggregate level in the soil of the maize site using the ¹³C/¹²C isotope ratio. SOM contents were higher in the grassland soil aggregates than in those of the arable soils mainly because of greater contents of mineral-associated SOM. The contribution of occluded POM to total SOC in the A horizon aggregates was greater in the spruce soil (23-44%) than in the grassland (11%) and arable soils (19%). The mass and carbon content of both the free and occluded POM fractions were greater in the forest soil than in the grassland and arable soils. In all soils, the C/N ratios of soil fractions within each aggregate size class decreased in the following order: free POM_<1.6>occluded POM_<1.6-2.0>mineral-associated SOM. The mean age of SOC associated with the <53 μm mineral fraction of water-stable aggregates in the Ap horizon of the maize site varied between 63 and 69 yr in aggregates >250 μm, 76 yr in the 53-250 μm aggregate class, and 102 yr in the sieve fraction <53 μm. The mean age of SOC in the occluded POM increased with decreasing aggregate size from 20 to 30 yr in aggregates >1000 μm to 66 yr in aggregates <53 μm. Free POM had the most rapid rates of C-turnover, with residence times ranging from 10 yr in the fraction >2000 μm to 42 yr in the fraction 53-250 μm. Results indicated that SOM in slaking-resistant aggregates was not a homogeneous pool, but consisted of size/density fractions exhibiting different composition and stability. The properties of these fractions were influenced by the aggregate size. Land cover/land use were important factors controlling the amount and composition of SOM fractions at the aggregate level.     

Organic matter in size‐density fractions after 16–50 years of grass ley,cereal cropping and organic amendments

Size‐density fractionation, which was originally developed to examine short‐term decomposition of added material in sandy soil, was highly sensitive to medium‐ to long‐term changes in loam and clay soils. Materials from different size classes (>1 mm, 0.1–1 mm, 0.05–0.1 mm and <0.05 mm) were separated by density into light (ρ < 1.0 g cm⁻³), medium (1.0 < ρ < 1.85 g cm⁻³) and heavy (ρ > 1.85 g cm⁻³) fractions. In 16–18‐year cropping experiments the 0.1–1‐mm heavy fraction contained 17–19% of total carbon in ley soils compared with 7% in continuously cropped soils. Greater N‐mineralization rates after sieving of ley cropped soils could not be related to differences in C:N ratios of fractions, but this was assumed to be related to exposure of aggregate‐binding agents. In a 50‐year trial 40% of total soil carbon was contained in the 0.1–1‐mm heavy fraction in soil treated with sewage sludge compared with 7–9% in the fallow and ‘zero N’ treatments. Thus, the soils studied expressed an aggregate hierarchy dependent on organic carbon dynamics. The relative abundance of carbon in heavy organo‐mineral fractions >0.1 mm was inversely related to the relative abundance of C in black‐brown medium density material <0.1 mm, defined as uncomplexed particulate organic matter that was presumably released during ultrasonic disruption deterioration of finer (<0.1 mm) aggregated organo‐mineral particles. The size density fractionation allowed identification of materials of contrasting visual appearance, chemical qualities and, by inference, biological turnover times. However, they were found to be predominantly composite fractions and may be too complex to be represented by unique model pools.     

Long-term fertilization and manuring effects on physically-separated soil organic matter pools under a wheat-wheat-maize cropping system in an arid region of China

With increasing food demand worldwide, agriculture in semiarid and arid regions becomes increasingly important, though knowledge about organic matter (OM) conserving management systems is scarce. This study aimed at examining organic C (OC) and nitrogen (N) concentrations in various soil OM pools affected by 26-years application of chemical fertilizer and farmyard manure at an arid site of Gansu Province, China. Macro OM (>0.05 mm) was extracted by wet sieving and then separated into light macro OM (<1.8 g cm⁻³) and heavy macro OM (>1.8 g cm⁻³) sub-fractions; bulk soil was differentiated into free particulate OM (FPOM, <1.6 g cm⁻³), occluded particulate OM (OPOM, 1.6-1.8 g cm⁻³) and mineral-associated OM (>1.8 g cm⁻³). OC and N concentrations of heavy macro OM and FPOM were slightly affected by long-term N fertilization alone and its combination with P and K, but their magnitudes of change had not significantly contributed to total soil OC and N concentrations. Farmyard manure increased light macro OC and N by 58 and 70%, heavy macro OC and N by 86 and 117%, free particulate OC and N by 29 and 55%, occluded particulate OC and N by 29 and 55%, and mineral-associated OC and N by 44 and 48%, respectively, compared to nil-manure. Mineral fertilization improved soil OM quality by decreasing C/N ratio in the light macro OM and FPOM fractions where farmyard manure was absent. Organic manure led to a decline of the C/N ratio in all physically-separated OM fractions possibly due to the increased input of processed organic materials. We found about two thirds of macro OM was actually located within 2-0.05 mm organo-mineral associations or/and aggregates. In conclusion, this study stresses the vital importance to apply organic manure to the wheat-corn production system characterized by straw removal and conventional tillage in the region.     

Tillage Effects on Particulate and Mineral‐Associated Organic Matter in Two Tropical Brazilian Soils

Abstract

Two Ferralsols (350 and 600 g kg^?1 clay) from the Brazilian Cerrado Region were evaluated for long‐term effects (5 and 8 years) of no tillage on carbon (C) stocks in particulate (>53 µm) and mineral‐associated (<53 µm) soil organic matter (SOM) fractions. Carbon stocks in particulate SOM increased under no tillage compared with conventional tillage, and the rate was higher in the clayey soil (0.62 Mg C ha^?1 yr^?1) than in the sandy clay loam soil (0.31 Mg C ha^?1 yr^?1). In contrast, the mineral‐associated SOM in the top soil layer (0–20 cm) was not affected by tillage system. Sequestration of atmospheric C in tropical no‐tillage soils seems to be due to accumulation of C in labile SOM fractions, with highest rates in clayey soils probably due to physical protection.     

SOIL ORGANIC CARBON STOCK AND FRACTIONS IN RELATION TO LAND USE AND SOIL DEPTH IN THE DEGRADED SHIWALIKS HILLS OF LOWER HIMALAYAS

The proportional differences in soil organic carbon (SOC) and its fractions under different land uses are of significance for understanding the process of aggregation and soil carbon sequestration mechanisms. A study was conducted in a mixed vegetation cover watershed with forest, grass, cultivated and eroded lands in the degraded Shiwaliks of the lower Himalayas to assess land‐use effects on profile SOC distribution and storage and to quantify the SOC fractions in water‐stable aggregates (WSA) and bulk soils. The soil samples were collected from eroded, cultivated, forest and grassland soils for the analysis of SOC fractions and aggregate stability. The SOC in eroded surface soils was lower than in less disturbed grassland, cultivated and forest soils. The surface and subsurface soils of grassland and forest lands differentially contributed to the total profile carbon stock. The SOC stock in the 1.05‐m soil profile was highest (83.5 Mg ha⁻¹) under forest and lowest (55.6 Mg ha⁻¹) in eroded lands. The SOC stock in the surface (0–15 cm) soil constituted 6.95, 27.6, 27 and 42.4 per cent of the total stock in the 1.05‐m profile of eroded, cultivated, forest and grassland soils, respectively. The forest soils were found to sequester 22.4 Mg ha⁻¹ more SOC than the cultivated soils as measured in the 1.05‐m soil profiles. The differences in aggregate SOC content among the land uses were more conspicuous in bigger water‐stable macro‐aggregates (WSA > 2 mm) than in water‐stable micro‐aggregates (WSA < 0.25 mm). The SOC in micro‐aggregates (WSA < 0.25 mm) was found to be less vulnerable to changes in land use. The hot water soluble and labile carbon fractions were higher in the bulk soils of grasslands than in the individual aggregates, whereas particulate organic carbon was higher in the aggregates than in bulk soils. Copyright © 2012 John Wiley & Sons, Ltd.     

Thirty-Year Manuring and Fertilization Effects on Heavy Metals in Black Soil and Soil Aggregates in Northeastern China

To evaluate the effects of thirty years of manure and chemical fertilizer applications on metal accumulations in soil and soil aggregates, fresh soils were separated by wet sieving into four aggregate fractions and heavy-metal concentrations in soil and aggregates were determined. The soil organic carbon (SOC) concentration in microaggregates ranged from 20.2 to 39.6 g carbon (C) kg^?1, which was significantly greater than those in the other fractions. The proportion of heavy metals in small macroaggregates and the silt + clay fraction accounted for 45.5 ± 10.6% and 35.8 ± 14.1% of the total amount in soil, respectively, which might be due primarily to their greater mass percentages in soil. Both chemical fertilizer and manure significantly stimulated iron (Fe) and zinc (Zn) accumulation; horse manure also increased copper (Cu), lead (Pb), and chromium (Cr) concentration in bulk soils as compared with the control. The results also indicated that heavy-metal distribution in aggregates was not controlled by SOC but possibly by soil clay.     

Separation of light and heavy organic matter fractions in soil — Testing for proper density cut-off and dispersion level

Density fractionation is frequently applied to separate soil organic matter according to the degree and the mode of interaction with minerals. Density fractions are operationally defined by density cut-off and sonication intensity, which determine the nature of the separated material. However, no tests or general agreements exist on the most appropriate density cut-off as well as on method and intensity of dispersion. Numerous variants have been proposed and applied, with results often contrasting each other and being hard to interpret. Here, we aimed at separating two light fractions (free and occluded into aggregates) composed of almost pure organic material, and one heavy fraction comprising the organic–mineral associations. We tested effects of different density cut-offs and sonication intensities, in combination and separately, on fraction yields, as well as on the fractions' organic carbon, total nitrogen and lignin-derived phenols. We tried to find optimum density cut-offs and sonication intensities, providing light fractions with maximum organic material and minimum contamination by mineral material. Under the test conditions, a density of 1.6 g cm^?3 gave best results for all test soils, allowing for separation of maximums amounts of almost pure organic material. The density cut-off at 1.6 g cm^?3 is well in line with previous studies and theoretical considerations, therefore we recommend the use of this density as most suitable for separation of organic debris. Sonication levels for aggregate disruption to achieve complete separation of occluded light organic matter varied amongst soils. The necessary intensity of dispersion relates to the type of soil, depending on the stability of contained aggregates. The application of one single dispersion energy level to different soils may result either in mineral contamination or in incomplete separation of light and heavy fractions as well as in redistribution of organic material amongst fractions. This means there is no single sonication level that can be applied to all soils. Thus, obtaining a meaningful light fraction residing within aggregates (occluded light fraction) requires assessment of the dispersion energy necessary to disrupt the aggregate system of a given soil without dispersion of organic–mineral associations. This can be done in pre-experiments where the soil is fractionated at different sonication levels. The appropriate dispersion is determined by mass yields and OC content of the obtained occluded fractions.     

Impact of tillage and crop rotation on light fraction and intra-aggregate soil organic matter in two Oxisols

It is well known that no-tillage (NT) practices can promote greater stocks of soil organic matter (SOM) in the soil surface layer compared to conventional tillage (CT) by enhancing the physical protection of aggregate-associated C in temperate soils. However, this link between tillage, aggregation and SOM is less well established for tropical soils, such as Oxisols. The objective of this study was to investigate the underlying mechanisms of SOM stabilization in Oxisols as affected by different crop rotations and tillage regimes at two sites in southern Brazil. Soils were sampled from two agricultural experiment sites (Passo Fundo and Londrina) in southern Brazil, with treatments comparing different crop rotations under NT and CT management, and a reference soil under native vegetation (NV). Free light fraction (LF) and intra-aggregate particulate organic matter (iPOM) were isolated from slaking-resistant aggregates. Of the total C associated with aggregates, 79–90% was found in the mineral fraction, but there were no differences between NT and CT. In contrast, tillage drastically decreased LF-C concentrations in the 0–5 cm depth layer at both sites. In the same depth layer of NT systems at Londrina, the concentrations of iPOM-C were greater when a legume cover crop was included in the rotation. At Londrina, the order of total iPOM-C levels was generally NV > NT > CT in the 0–5 cm depth interval, but the difference between NT and CT was much less than in Passo Fundo. At Passo Fundo, the greatest concentrations and differences in concentrations across tillage treatments were found in the fine (53–250 μm) iPOM fractions occluded within microaggregates. In conclusion, even though no aggregate hierarchy exists in these Oxisols, our results corroborate the concept of a stabilization of POM-C within microaggregates in no-tillage systems, especially when green manures are included in the rotation.     

长期免耕对东北地区玉米田土壤有机碳组分的影响

Increasing evidence has shown that conservation tillage is an effective agricultural practice to increase carbon (C) sequestration in soils. In order to understand the mechanisms underlying the responses of soil organic carbon (SOC) to tillage regimes, physical fractionation techniques were employed to evaluate the effect of long-term no-tillage (NT) on soil aggregation and SOC fractions. Results showed that NT increased the concentration of total SOC by 18.1% compared with conventional tillage (CT) under a long-term maize (Zea mays L.) cropping system in Northeast China. The proportion of soil large macroaggregates ( 2000 μm) was higher in NT than that in CT, while small macroaggregates (250-2000 μm) showed an opposite trend. Therefore, the total proportion of macroaggregates ( 2000 and 250-2000 μm) was not affected by tillage management. However, C concentrations of macroaggregates on a whole soil basis were higher under NT relative to CT, indicating that both the amount of aggregation and aggregate turnover affected C stabilization. Carbon concentrations of intra-aggregate particulate organic matter associated with microaggregates (iPOM m) and microaggregates occluded within macroaggregates (iPOM mM) in NT were 1.6 and 1.8 times greater than those in CT, respectively. Carbon proportions of iPOM m and iPOM mM in the total SOC increased from 5.4% and 6.3% in CT to 7.2% and 9.7% in NT, respectively. Furthermore, the difference in the microaggregate protected C (i.e., iPOM m and iPOM mM) between NT and CT could explain 45.4% of the difference in the whole SOC. The above results indicate that NT stimulates C accumulation within microaggregates which then are further acted upon in the soil to form macroaggregates. The shift of SOC within microaggregates is beneficial for long-term C sequestration in soil. We also corroborate that the microaggregate protected C is useful as a pool for assessing the impact of tillage management on SOC storage.     

Rapid incorporation of carbon from fresh residues into newly formed stable microaggregates within earthworm casts

Earthworms play an important role in protecting carbon in the soil, but the exact influence of their activity on the distribution and protection of C is still poorly understood. We investigated the effect of earthworms on the formation of stable microaggregates inside newly formed macroaggregates and the distribution of C in them. We crushed (< 250 µm) soil, and subjected it to three treatments: (i) soil + ¹³C‐labelled residue + earthworms (these added after 8 days' incubation), (ii) soil + ¹³C‐labelled residue, and (iii) control (no additions), and then incubated it for 20 days. At the end, we measured the aggregate size distribution, total C and ¹³C, and we isolated microaggregates (53–250 µm) from macroaggregates (> 250 µm) formed. The ¹³C in fine particulate organic matter between and within the microaggregates was determined. Earthworms helped to form large macroaggregates (> 2000 µm). These large macroaggregates contained four times more stable microaggregates than those from samples without earthworms. There was more particulate organic matter within and between microaggregates in macroaggregates in the presence of earthworms. The larger amounts of organic matter inside stable microaggregates in casts than in bulk soil after 12 days of incubation (140 mg ¹³C kg^?1 soil compared with 20 mg ¹³C kg^?1 soil) indicates that these microaggregates are formed rapidly around freshly incorporated residues within casts. In conclusion, earthworms have a direct impact on the formation of stable microaggregates and the incorporation of organic matter inside these microaggregates, and it seems likely that their activity is of great significance for the long‐term stabilization of organic matter in soils.     

Long‐term cropping systems and tillage management effects on soil organic carbon stock and steady state level of C sequestration rates in a semiarid environment

A calcareous and clayey xeric Chromic Haploxerept of a long‐term experimental site in Sicily (Italy) was sampled (0–15 cm depth) under different land use management and cropping systems (CSs) to study their effect on soil aggregate stability and organic carbon (SOC). The experimental site had three tillage managements (no till [NT], dual‐layer [DL] and conventional tillage [CT]) and two CSs (durum wheat monocropping [W] and durum wheat/faba bean rotation [WB]). The annually sequestered SOC with W was 2·75‐times higher than with WB. SOC concentrations were also higher. Both NT and CT management systems were the most effective in SOC sequestration whereas with DL system no C was sequestered. The differences in SOC concentrations between NT and CT were surprisingly small. Cumulative C input of all cropping and tillage systems and the annually sequestered SOC indicated that a steady state occurred at a sequestration rate of 7·4 Mg C ha⁻¹ y⁻¹. Independent of the CSs, most of the SOC was stored in the silt and clay fraction. This fraction had a high N content which is typical for organic matter interacting with minerals. Macroaggregates (>250 µm) and large microaggregates (75–250 µm) were influenced by the treatments whereas the finest fractions were not. DL reduced the SOC in macroaggregates while NT and CT gave rise to higher SOC contents. In Mediterranean areas with Vertisols, agricultural strategies aimed at increasing the SOC contents should probably consider enhancing the proportion of coarser soil fractions so that, in the short‐term, organic C can be accumulated. Copyright © 2010 John Wiley & Sons, Ltd.     

Distribution of light and heavy fractions of soil organic carbon as related to land use and tillage practice

Mass distributions of different soil organic carbon (SOC) fractions are influenced by land use and management. Concentrations of C and N in light- and heavy fractions of bulk soils and aggregates in 0–20 cm were determined to evaluate the role of aggregation in SOC sequestration under conventional tillage (CT), no-till (NT), and forest treatments. Light- and heavy fractions of SOC were separated using 1.85 g mL⁻¹ sodium polytungstate solution. Soils under forest and NT preserved, respectively, 167% and 94% more light fraction than those under CT. The mass of light fraction decreased with an increase in soil depth, but significantly increased with an increase in aggregate size. C concentrations of light fraction in all aggregate classes were significantly higher under NT and forest than under CT. C concentrations in heavy fraction averaged 20, 10, and 8 g kg⁻¹ under forest, NT, and CT, respectively. Of the total SOC pool, heavy fraction C accounted for 76% in CT soils and 63% in forest and NT soils. These data suggest that there is a greater protection of SOC by aggregates in the light fraction of minimally disturbed soils than that of disturbed soil, and the SOC loss following conversion from forest to agriculture is attributed to reduction in C concentrations in both heavy and light fractions. In contrast, the SOC gain upon conversion from CT to NT is primarily attributed to an increase in C concentration in the light fraction.     

Contributions of soil biota to C sequestration varied with aggregate fractions under different tillage systems

It is increasingly believed that substantial soil organic carbon (SOC) can be sequestered in conservation tillage system by manipulating the functional groups of soil biota. Soil aggregates of different size provide diverse microhabitats for soil biota and consequently influence C sequestration. Our objective was to evaluate the contributions of soil biota induced by tillage systems to C sequestration among different aggregate size fractions. Soil microbial and nematode communities were examined within four aggregate fractions: large macroaggregates (>2 mm), macroaggregates (2–1 mm), small macroaggregates (1–0.25 mm) and microaggregates (<0.25 mm) isolated from three tillage systems: no tillage (NT), ridge tillage (RT) and conventional tillage (CT) in Northeast China. Soil microbial and nematode communities varied across both tillage systems and aggregate fractions. The activity and abundance of microbes and nematodes were generally higher under NT and RT than under CT. Among the four aggregate fractions, soil microbial biomass and diversity were higher in microaggregates, while soil nematode abundance and diversity were higher in large macroaggregates. Structural equation modelling (SEM) revealed that the linkage between microbial and nematode communities and their contributions to soil C accumulation in >1 mm aggregate fractions were different from those in <1 mm aggregate fractions. Higher abundance of arbuscular mycorrhizal fungi (AMF) could enhance C retention within >1 mm aggregates, while more gram-positive bacteria and plant-parasitic nematodes might increase C accumulation within <1 mm aggregates. Our findings suggested that the increase in microbial biomass and nematode abundance and the alteration in their community composition at the micro-niche within aggregates could contribute to the higher C sequestration in conservation tillage systems (NT and RT).