用微小隐孢子虫子孢子表面蛋白DNA疫苗免疫小鼠的实验研究

为了探讨微小隐孢子虫子孢子表面蛋白 CP15 / 60重组质粒 pc DNA3 .1- 15 / 60 DNA疫苗诱导机体产生体液和细胞免疫应答的效果。用重组的 DNA疫苗于 BAL B/ c小鼠后腿胫骨前肌肉注射免疫 ,于 0、3、6周共免疫 3次 ,10 0 μg/次。免疫后不同时间检测体液和细胞免疫应答指标。并用 1× 10 6 卵囊进行攻虫试验。结果表明 pc DNA 3 .1- 15 / 60可诱导机体产生相应的特异性抗体 ,对 C.parvum卵囊攻击具有保护作用。微小隐孢子虫子孢子表面蛋白 CP15 / 60重组质粒 pc DNA3 .1- 15 / 60有可能作为侯选的隐孢子虫 DNA疫苗 ,值得进一步深入研究     

N-糖基化猪瘟病毒E2蛋白不同接种剂量对BALB/c小鼠的免疫效果分析

E2蛋白（E2 protein,E2-Pro）为猪瘟病毒（classical swine fever virus,CSFV）的主要结构蛋白之一,是CSFV最主要的保护性抗原,因此应用体外糖基化技术获得的N-糖基化E2蛋白（glycosylated E2 protein,Gly-E2-Pro）理论上可进一步增强机体的免疫应答。为了验证这一设想以及获得最佳的免疫效果,本研究分别将Gly-E2-Pro按20,50,100μg/只,接种BALB/c小鼠,同时设E2-Pro与CSFV对照,于不同时间采血制备血清,通过血清中IgG、IL-4及INF-γ水平的测定,评价Gly-E2-Pro的免疫效果及确定最佳免疫剂量。结果显示,免疫Ⅰ组（20μg/只）Gly-E2-Pro虽刺激机体产生了IgG免疫应答,但水平较低,与E2-Pro、CSFV组差异不显著;免疫Ⅲ组（100μg/只）Gly-E2-Pro免疫后,前期出现免疫抑制,至28d抗体水平急剧上升,达到极高值;免疫Ⅱ组（50μg/只）Gly-E2-Pro免疫后,血清IgG水平显著高于E2-Pro与CSFV组,同时显著高于免疫Ⅰ组与Ⅲ组。以上结果说明,Gly-E2-Pro可有效增强机体的体液免疫应答,50μg/只为最佳接种剂量。     

I. Immunosurveillance and tumorigenesis in BALB/c mice—1. Early reactive state in mesenteric granulomas

The cellular immune response in BALB/c mice mesenteric granulomas (MG) was analysed by the means of functional ultrastructure and the assessment of the presence or absence of asynchronous plasma cells in situ.

Results from this study establish: (a) the sequential events involved in the genesis of the oil-induced granulomas, (b) the weak T-cellular immune response and the almost absence of these cells in mitosis, (c) the presence of asynchronous plasma cells of type II was found in mice Nos. 8, 9, 10 and 11 and they were considered to be plasma cell mutants of temporary type, to indicate the first step of this cell alienation in tumorigenesis, (d) the high ratio of peritoneal macrophages was found and connected with what is known about it in the literature. It was speculated that the supraoptimal presence of peritoneal macrophages could have been responsible for the appearance of plasma cell mutants and (e) four morphological types of macrophages have been found in this animal's oil-induced MG, representing different stages of the monocyte transformation in situ.