Quantification of physical (roughness) and chemical (dielectric constant) leaf surface properties relevant to wettability and adhesion

BACKGROUND: Spray droplet adhesion is dependent not only on formulation and droplet parameters but also on the surface properties (physical and chemical) of the leaf. Quantifying these leaf surface properties would aid understanding and modelling of adhesion, helping to optimise spray formulations. Fractal dimensions (FDs) were used to quantify the relative leaf surface roughness of ten plant species. Static droplet contact angles were measured on each leaf surface, and wetting tension was calculated. Chemical profiles of the leaf surfaces were developed by evaluating contact angle behaviour relative to solution dielectric constants. RESULTS: The FDs of Cryo‐SEM micrographs taken at 300× magnification gave the best correlation with adhesion. The wetting tension intercept had a strong relationship with mean adhesion, and successfully accounted for the wettability of the outlier species. CONCLUSIONS: The microroughness of the leaf surface, as revealed by Cryo‐SEM, can be quantified by fractal dimension analysis. However, the wetting tension intercept is a more useful universal measure of the surface properties of the leaf (including roughness) as they pertain to adhesion. The slope of the wetting tension versus dielectric constant plot allowed preliminary quantification of the chemical contribution of leaf surface dielectric behaviour to adhesion. Copyright © 2011 Society of Chemical Industry     

Finite dose diffusion studies: II. Effect of concentration and pH on NAA penetration through isolated tomato fruit cuticles

The effects of NAA [2‐(1‐naphthyl)acetic acid] concentration and pH on penetration of NAA from aqueous droplets (5 µl) through isolated tomato (Lycopersicon esculentum) fruit cuticles were studied using a finite dose diffusion system. Penetration time‐courses were characterized by a lag phase, which generally extended beyond the time of droplet drying. Initially penetration rates increased, reached a maximum penetration rate, remained constant for several hours, and then decreased with time. Penetration approached a plateau within 120 h after droplet application. Increasing NAA concentration in the donor droplet increased NAA penetration in both the presence and absence of the citric acid buffer (20 mM , pH 3.2). Maximum rates of penetration and the total amount of NAA that penetrated at 120 h were both linearly related to the initial concentration of the donor droplet (ranging from 0.001 to 0.1 mM NAA). The apparent first‐order rate constants for the concentration dependence of NAA penetration rates were greater with buffer than without (0.94 × 10⁻⁸ vs 0.50 × 10⁻⁸ m s⁻¹, respectively). While pH of the receiver solution (pH 6.2 vs 2.2) did not affect penetration, decreasing donor pH from 6.2 to 2.2 increased NAA penetration at 120 h. © 2000 Society of Chemical Industry     

甲氨基阿维菌素苯甲酸盐药液浓度、雾滴密度及施药液量对小菜蛾防治效果的影响

采用室内生物测定与田间试验相结合方法,研究了甲氨基阿维菌素苯甲酸盐药液浓度、雾滴密度和施药液量对小菜蛾防治效果间的关系。结果表明:当甲氨基阿维菌素苯甲酸盐药液质量浓度从80 mg/L提高至640 mg/L时,其LN50值(致死中密度)从148 droplet/cm2下降至3 droplet/cm2;雾滴密度从23 droplet/cm2提高至131 droplet/cm2时,其LC50(致死中浓度)值则从1.66!102mg/L下降至78.93 mg/L。甲氨基阿维菌素苯甲酸盐在与地面呈0°倾角的载玻片正面和90°倾角的载玻片反面上沉积量最多,在45°倾角的反面和90°倾角的正面上沉积量最少;在甘蓝叶片上的沉积量与药液质量浓度和雾滴大小有关。田间药效试验结果表明:甲氨基阿维菌素苯甲酸盐药液质量浓度为3 mg/L时,施药液量为330 L/hm2的防治效果比施药液量分别为525、750和975 L/hm2的低,差异达显著水平(P0.05),但后3种施药液量之间的防治效果差异不显著(P0.05);当药液质量浓度为4.2 mg/L时,330、525、750和975 L/hm24种施药液量间的防治效果差异均不显著(P0.05)。因此,采用较小雾滴体积中径(VMD=128.9μm)和较高药液浓度(4.2 mg/L)喷雾时,甲氨基阿维菌素苯甲酸盐不仅对小菜蛾表现出优良的防治效果,而且施药液量也显著降低。     

Effect of botanical extracts on the biological activity of granulosis virus against <Emphasis Type="Italic">Pieris brassicae</Emphasis>

A granulosis virus strain infecting Pieris brassicae (PbGV) was isolated from the dry temperate region of northwestern Himalayas as a potential microbial agent for its management. The effect of different botanicals (having insecticidal action against P. brassicae) on the bioefficacy of PbGV was evaluated under laboratory conditions using leaf disc bioassays on cabbage for improving the insecticidal performance of the PbGV. The synergistic action of different botanical extracts was evident in terms of reduction in LC₅₀ values against different botanical extracts. Among different extracts, petroleum-ether extract of neem seed kernel (NSK) when combined with PbGV resulted in maximum reduction of LC₅₀ value (4.39 × 10² occlusion bodies [OBs] ml⁻¹) followed by methanolic extract (7.38 × 10² OBs ml⁻¹) and aqueous extract (9.36 × 10³ OBs ml⁻¹) as compared with PbGV alone (1.85 × 10⁴ OBs ml⁻¹) for 2nd instar larvae of the test insect. These trends were found analogous in cases of 3rd and 4th instars of P. brassicae with different solvent extracts of NSK. The other botanicals evaluated, viz., Eupatorium and Artemesia, also resulted in reduction of LC₅₀ values for 2nd, 3rd and 4th instars as compared with PbGV alone when different extracts were combined with virus for bioassays. The studies suggest that the PbGV in combination with botanical pesticides could be more useful as a bio-pesticide against cabbage butterfly (P. brassicae) in IPM programs.     

Interaction Between Entomopathogenic Fungi and Some Aphid Species in Thailand

Seven aphid species were screened with twelve entomopathogenic fungi for the selection of the most effective fungus. Beauveria bassiana CKB-048 caused the highest mortalities for all aphid species tested in their nymphal stage. The cowpea aphid (Aphis craccivora Koch) was chosen for further studies in its adult stage using B. bassiana CKB-048 as a biocontrol agent, and carbosulfan was used for a comparison in greenhouse testing. The mode of action of the fungal killing was studied by scanning electron microscopy. The endophytic colonization of the cowpea plant by B. bassiana CKB-048 was studied and confirmed using both stereo microscopy and transmission electron microscopy. The chitinase enzyme activity of cowpea plants after they were sprayed with the fungus or carbosulfan was found to fluctuate throughout five days. The lethal concentrations of B. bassiana CKB-048 suspensions that killed 50 % (LC₅₀) of A. craccivora was 6.69 × 10⁷ conidia/ml for the nymphs and 8.25 × 10⁷ conidia/ml for the adults. The time for lethality (LT₅₀) with a concentration of 2 × 10⁸ conidia/ml was 3.25 days for nymphs and 4 days for adults.     

五台山地区森林生态系统服务功能价值评估

森林对维持我国自然生态系统的平衡和稳定具有重要意义,其服务功能价值越来越受到人们的重视。采用综合评价方法对五台山地区森林生态系统服务功能价值进行估算。结果表明:五台山地区森林生态系统服务功能总价值为101.47×108元,单位面积价值量为6.80元·m-2·a-1,其中供给功能价值为0.44×108元(占0.43%),支持功能价值为31.34×108元(占30.89%),调节功能价值为65.83×108元(占64.88%),文化服务功能价值为3.86×108元(占3.80%);从不同的服务功能类型来看,其价值量为:防风固沙维持营养物质循环涵养水源气候调节保护生物多样性休憩娱乐净化空气提供林产品控制侵蚀;不同森林类型价值量差异较大,灌丛最大,针阔混交林最小,从单位面积价值量来看,松杉类最大,灌丛最小;五台山地区森林生态系统服务功能价值巨大,该结果对区域生态系统管理具有重要意义。     

酒泉盆地地下水系统水资源评价

在系统分析研究区地质及水文地质条件的基础上,确定了研究区范围和边界条件,借助GIS技术与FEFLOW建立了研究区地下水系统概念模型和数学模型及相应的地下水系统数值模型。以2001~2003年地下水动态观测井的地下水动态观测资料,对建立的模型进行了多次求解与识别,结果表明:建立的模型能够较好的反映研究区水文地质条件空间分布及组合方式,具有一定的代表性可以用来对研究区地下水资源进行定量评价与预测。运用识别后的模型评价和预测了在P=50%、75%、95%不同地表径流条件下研究区地下水系统水资源量,与其对应的补给量分别为7.7346亿m3/a、7.1663亿m3/a、6.3610亿m3/a,排泄量分别为:8.7208亿m3/a、8.3789亿m3/a、7.8477亿m3/a,总均衡分别为:-0.9862亿m3/a、-1.2126亿m3/a、-1.4867亿m3/a。结果表明在上述三种不同典型年情况下,研究区地下水系统长期处于负均衡状态,为了改善该区域脆弱的生态环境,地下水开采前应进行可开采量论证,并制定合理的地下水开采方案。     

The resistance to eighteen toxicants of a strain of Musca domestica L. collected from a farm in England

A sample of houseflies initially collected from a pig farm and found to be resistant to bendiocarb, DDT, gamma-HCH, pyrethrins + piperonyl butoxide (PB), tetrachlorvinphos and trichlorfon, was tested for resistance to knockdown by other toxicants. At the KD₅₀ response level, resistance factors were obtained for: permethrin (× 141), deltamethrin (×96), bioresmethrin + PB (×37), resmethrin + PB (×33), fenitrothion (×94), bromophos (×58), iodofenphos (×42), pirimiphos-methyl (×30), dichlorvos (×22), dimethoate (×9), diazinon (×8), methomyl (×4) and methomyl + PB (×4). The slopes of the dose–response lines were lower for the farm strain than for a susceptible strain. This resulted in an increase of resistance factors at the KD₉₅ level by an average of × 1.6. The houseflies on the farm could not be controlled using space sprays of pyrethrins + PB, although resistance to this toxicant was only ×12. However, control was achieved with a methomyl bait.     

表面活性剂、喷液量及杂草叶角对普杀特喷后附着量的影响

表面活性剂、喷液量及杂草叶角对普杀特在非敏感杂草马唐叶片上的喷后附着量有明显的独立影响和交互影响,其中表面活性剂的影响最大。供试的6种非离子型表面活性剂均因显著降低了普杀特药液的表面张力从而显著提高了其在马唐叶片上的喷后附着量,其中ScOil和Silwet—L77对其提高幅度最大;普杀特在马唐叶片上的喷后附着量随着喷液量的减少逐步增加,但在0—90°范围内随着马唐叶角的减小而减少,其由于喷液量的增大或马唐叶角的减小而引起的下降量,可通过加入非离子型表面活性剂而得到补偿,加入非离子型表面活性剂配合下午微液量(90L/ha)喷施,可望最大限度地增加普杀特在其非敏感杂草叶片上的喷后附着量,进而提高其药效,降低其使用成本和减少其在土壤中的残留。     

Effect of Weeding Regime and Plant Spacings on Weed Growth and Performance of Transplanted Rice

Abstract

Effect of weeding regime (unweeded control, hand weeding once, 4 weeks after transplanting (w.a.t.) and hand weeding twice, 4 and 7 w.a.t.) and different plant spacings (20 × 2.5 cm, 20 × 5 cm, 20 × 10 cm, 20 × 15 cm and 20 × 20 cm) on weed growth and performance of transplanted rice were tested in a split-plot design. Grain yield and number of panicles were not significantly different in both weeding treatments from the unweeded control at 20 × 2.5 to 20 × 10 cm spacings, showing that, at closer spacings weed control may not be necessary to increase grain yield. The number of weeds at harvest was significantly lower in the weeded plots than the unweeded control at all spacings used. Weed weight at harvest was significantly affected by weeding regime at all spacings except at 20 × 2.5 cm, where no significant difference was observed between the weeded and the unweeded plots. Plant spacings significantly affected the grain yield and weed weight, but did not affect the panicle number and weed counts of transplanted rice. At 20 × 2.5 cm, grain yield was significantly better than that at 20 × 15 cm or 20 × 20 cm. A simple trend of increase in grain yield along with the decrease in spacing between plants was observed in terms of actual grain yield.     

枯草芽胞杆菌GB519在水稻植株中的定殖及对稻瘟病田间防效

枯草芽胞杆菌GB519是一株具有广谱抑菌活性的生防菌株。本研究利用绿色荧光蛋白标记的菌株GB519-GFP处理水稻种子、根和叶片,结合激光共聚焦显微镜观察和抗生素平板回收检测的方法,探究其在水稻根茎叶中的定殖动态。结果显示：经GB519-GFP发酵液处理水稻种子、根和叶片后,菌株均可内生定殖于植株的表皮、皮层和维管束中,表明其可在水稻植株内迁移和定殖。GB519-GFP在处理部位的定殖量通常呈现先减少后增多的趋势,非处理部位3～5 d后即可检测到标记菌株。浸种处理,3 d后在幼芽中可检测到标记菌株;20 d后在根中的菌量最多,达5.7×10⁵ cfu/g。灌根处理,1 d后根中菌量为5.4×10⁵ cfu/g; 20 d后根、茎和叶中菌量均达到最大值;处理80 d后,根中定殖数量仍达1.9×10⁵ cfu/g。叶面喷施处理,1 d后叶片菌量为4.2×10⁵ cfu/g; 20 d后叶片菌量达4.4×10⁵ cfu/g。不同处理方法在各部位的定殖量几乎均在处理20 d后达到峰值。...     

The effect of molasses on the evaporation and impaction of spray droplets

The addition of 20% molasses to water did not reduce the rate of evaporation of water from spray droplets. The 20% molasses droplets evaporated at the same rate as water droplets until ca 20% of their volume was left. When sprayed under field conditions at 30% relative humdity (r.h.) the volume median diameter (v.m.d.) of the 20% molasses spray (132 μm) was larger than the v.m.d. of the water spray (116 μm) and more droplets smaller than 19 μm were captured on magnesium oxide slides than with the water spray. The addition of molasses improved the impaction efficiency of droplets by increasing their density, and increased the minimum size to which a given droplet could evaporate.     

内蒙古黄土高原区降水规律与集雨利用潜力分析

在黄土高原干旱少雨,地下水埋深较深的地区,通过建立水窖集雨供人畜饮用和作物补充灌溉具有重要意义。本研究利用沙圪堵镇的准格尔旗气象站1959—2000年气象资料,进行降水规律分析。结果表明,位于内蒙古黄土高原区的准格尔旗年均降水量为392.1mm,最大年降水量为635.5mm,最小降水量仅为142.5mm。年降水量在500mm以上的保证率为19%,接近五年一遇,年降水量400mm以上的保证率为44%,可基本上满足短季耐旱作物的生长之需;降水量在300mm之下的频率为21%,不能满足作物生长对水分的需求。有1/3的年份年降水量300—400mm之间,作物生长所需水分亏缺的部分,需要适当补灌。由于4—5月作物播种和苗期降水量少,存在生长障碍,如能在前一年集雨次年春天补充灌溉将有利于全苗和增产。对于10mm以上的降水,保证率80%以上的降水量仅为135mm,保证率60%的降水量为200mm。中雨雨量为达到300mm的保证率只有30%。对于大雨过程,总降水量200mm只有15%的可能性,100mm以上的保证率为60%。如果降水10mm以上才能集到雨水,100m2的集雨面,只有60%的年份产水量大于20方。不同集雨面的集流效益不同,在建立集雨水窖时,要根据水窖体积和集雨面材料的集流效率,合理确定集雨面,保证80%以上的年份达到满意的集雨效果。另外,根据降水规律可利用自然地面进行田间集雨,利用土壤库容保存降水供作物生产之用。     

库布齐沙漠机械防沙措施的防护效益

在库布齐沙漠风沙防治试验场,开展了机械防沙措施的防护效益观测。结果表明:防风效益、阻沙效益和表土含水率等随沙障材料、规模和结构发生变化。1 1 m×1 m植物纤维网沙障和高密度聚乙烯(HDPE)网沙障风速降低比(K值)分别为58.5%和55.7%,均高于同规格的沙柳沙障的18.3%,说明同一规格下植物纤维网防风效果要优于其他材料;2 1 m×1 m植物纤维网沙障和1 m×1 m HDPE沙障的K值均小于相同材料8 m×8 m沙障的K值,说明小规格低立式沙障防风效应,低于相同材料较大规格高立式沙障;3月积沙量数据分析表明,阻沙栅栏能有效减小积沙量,而固沙沙障内积沙量受风向和局部地形影响较大;4不同材料与不同规格沙障内的表土含水率均高于对照区裸沙地,说明防沙措施对保持土壤水分也起到了一定作用。     

Fusarium solani f. sp. cucurbitae race 1, a potential pathogen of grafted watermelon production in Spain*

A crown, root and fruit rot of squash (Cucurbita maxima and Cucurbita moschata) has been observed in eastern provinces of Spain over the past 4 years. Isolations from the crown of symptomatic plants and fruits yielded primarily a Fusarium solani that was identified as F. solani f. sp. cucurbitae race 1 on the basis of pathogenicity tests and disease symptoms in the field. In Spain, more than 90% of watermelon plants are grafted, using different Cucurbita hybrids (C. maxima × C. moschata) as rootstocks. In 1998, some grafted watermelon plants were first found to be affected by F. s. cucurbitae race 1. The purpose of this study was to evaluate the pathogenicity of this fungus on several rootstocks commonly used for grafting watermelon (Brava, Titan, Shintoza, RS‐841, TZ‐148 and TW‐1) in order to prevent a possible spread of this fungus that could cause serious economic losses in watermelon production. None of them proved to be resistant.     

Effects of surfactants and the kinetic energy of monodroplets on the deposit structure of glyphosate at the micro‐scale and their relevance to herbicide bio‐efficacy on selected weed species

The deposit pattern of foliar‐applied agrochemicals, and its relation to their bio‐efficacy, has major practical importance. Thus, in our experiments, we evaluated the relevance of the deposition properties of glyphosate for its bio‐efficacy. The deposition pattern of glyphosate monodroplets was influenced by using surfactant and by applying the droplets with or without kinetic energy to the plant foliage. Monodroplets (1 μL) of glyphosate, formulated with or without ethoxylated rapeseed oil surfactant (RSO) having on average 5, 10, 30 or 60 ethylene oxide units (EO), as well as one commercial glyphosate product (CGP), were applied either by carefully placing the droplet on the foliage with a pipette (kinetic energy assumed to be near zero) or by a monodroplet generator (with kinetic energy). We selected two easy‐to‐wet (Stellaria media and Viola arvensis) and two difficult‐to‐wet (Chenopodium album and Setaria viridis) weed species as target plants. The deposit structure was determined using a scanning electron microscope with energy dispersive x‐ray microanalysis. The kinetic energy of the droplet had no consistent effect on the deposit structure or the bio‐efficacy of glyphosate formulations. In contrast, surfactants differing in EO unit, affected both the deposit structure and the bio‐efficacy of the formulations, depending upon the species. In easy‐to‐wet species, the increase in EO unit of RSO surfactant failed to affect the deposit area of glyphosate and its bio‐efficacy. However, in difficult‐to‐wet species, the increase in EO unit of RSO surfactant reduced the deposit area of glyphosate and enhanced its bio‐efficacy.     

防治西葫芦和黄瓜白粉病的生物制剂的筛选

瓜类白粉病是蔬菜上的重要病害之一,为加大对该病害的生物防治力度,本研究采用课题组研发的1×109cfu/g玫瑰黄链霉菌水剂和10亿芽胞/g枯草芽胞杆菌悬浮剂以及市场上常用的枯草芽胞杆菌、哈茨木霉菌、寡雄腐霉、武夷菌素、多抗霉素等多种生物制剂进行田间药效试验,比较几种生物制剂的防治效果。结果表明,供试生物制剂对西葫芦白粉病的防效为57.65%~84.98%,其中3%多抗霉素水剂600倍液的防效最好,为84.98%,且具有明显的促生长作用,增产率达12.65%。其次为1 000亿芽胞/g枯草芽胞杆菌可湿性粉剂(武汉天惠)400倍液、2%武夷菌素水剂600倍液和3×108 cfu/g哈茨木霉菌可湿性粉剂300倍液,可作为西葫芦白粉病防治的选用药剂。1×109 cfu/g玫瑰黄链霉菌水剂对西葫芦和黄瓜白粉病均具有较好的防效,分别为78.68%和73.59%,具有开发和应用的市场价值。     

Neem oil (Azadirachta indica) nanoemulsion--a potent larvicidal agent against Culex quinquefasciatus

BACKGROUND: Nanoemulsion composed of neem oil and non‐ionic surfactant Tween 20, with a mean droplet size ranging from 31.03 to 251.43 nm, was formulated for various concentrations of the oil and surfactant. The larvicidal effect of the formulated neem oil nanoemulsion was checked against Culex quinquefasciatus. RESULTS: O/W emulsion was prepared using neem oil, Tween 20 and water. Nanoemulsion of 31.03 nm size was obtained at a 1:3 ratio of oil and surfactant, and it was found to be stable. The larger droplet size (251.43 nm) shifted to a smaller size of 31.03 nm with increase in the concentration of Tween 20. The viscosity of the nanoemulsion increased with increasing concentration of Tween 20. The lethal concentration (LC₅₀) of the nanoemulsion against Cx. quinquefasciatus was checked for 1:0.30, 1:1.5 and 1:3 ratios of oil and surfactant respectively. The LC₅₀ decreased with droplet size. The LC₅₀ for the ratio 1:3 nanoemulsions was 11.75 mg L^?1. CONCLUSION: The formulated nanoemulsion of 31.03 nm size was found to be an effective larvicidal agent. This is the first time that a neem oil nanoemulsion of this droplet size has been reported. It may be a good choice as a potent and selective larvicide for Cx. quinquefasciatus. Copyright © 2011 Society of Chemical Industry     

梨园气溶胶中梨火疫病菌的定量检测

为系统研究梨园气溶胶中梨火疫病菌的含量, 本研究于2019年－2021年在新疆库尔勒市人和农场梨园, 利用病原菌孢子捕捉器在每年春季(4月下旬)、夏季(6月中旬)、秋季(9月中旬)收集梨园气溶胶, 检测梨火疫病菌。结果显示, 健康梨园气溶胶中未检测到梨火疫病菌, 不同发病程度的梨园气溶胶中均能检测到梨火疫病菌, 携菌量均值在102 cfu/(24cm2·h)以上, 其中, 气溶胶中梨火疫病菌含量最高值为2.81×104 cfu/(24cm2·h), 最低值为8.50×102 cfu/(24cm2·h); 重度、中度、轻度发病果园收集的气溶胶中含梨火疫病菌总菌落数均值分别为8.74×103、4.55×103、2.36×103 cfu/(24cm2·h)。此外, 在同一高度收集的气溶胶中, 梨火疫病菌菌落数随收集时间的延长而增加。不同季节气溶胶携菌量检测结果表明, 秋季发病梨园中气溶胶携菌量明显高于夏季和春季, 与梨园梨火疫病发病规律相符。致病性测定结果表明, 气溶胶中分离的梨火疫病菌具有致病性。     

10th British Weed Control Conference

The performance of different spray droplet spectra of cypermethrin from cone nozzles applied to brassica leaves was evaluated by bioassay using Plutella xylostella larvae. A spray droplet spectrum containing small droplets (VMD = 119 μm) caused a significantly higher knockdown and mortality compared to a coarser spray (VMD = 210 μm). No significant difference in the responses was observed when cypermethrin was sprayed over the top of the plants compared to plants sprayed from both sides using either nozzle; but the fine spray consistently gave better knockdown and mortality regardless of spray direction. The results indicated that spray effectiveness of cypermethrin could be improved by using a fine spray nozzle.