Physicochemical properties of natural phenolics from grapes and olive oil byproducts and their antioxidant activity in frozen horse mackerel fillets

The reducing and chelating capacities and the affinity for the incorporation into the fish muscle of grape procyanidins, hydroxytyrosol, and propyl gallate were studied together with their antioxidant activity in frozen horse mackerel (Trauchurus trauchurus) fillets. Fillets were supplemented with phenolic antioxidants by (a) spraying an aqueous phenolic solution, (b) glazing with an aqueous phenolic solution, and (c) a previous washing of fillets with water plus spraying an aqueous phenolic solution. The effect of washing on the endogenous pro-oxidant/antioxidant balance of the fillets was also determined. All phenolic compounds were effective delaying lipid oxidation in the fish fillets. The order of antioxidant efficiency in spraying and glazing was propyl gallate > hydroxytyrosol > procyanidins, which was similar to the reducing power of these phenolics, but did not show any correlation with their chelating capacity and their affinity to the fish muscle. Washing the fillets with water prior to spraying phenols increased synergistically the antioxidant activity of grape procyanidins and changed the relative antioxidant efficiency to propyl gallate approximately procyanidins > hydroxytyrosol. This synergism may be a result of a better distribution of the procyanidins onto the fillet surface because of the residual water that remained on the fillets surface after washing.     

Humic materials from an organic soil: A comparison of extractants and of properties of extracts

Five extraction procedures and thirteen extracting reagents, which included dipolar aprotic solvents, organic chelating agents, pyridine, ethylenediamine, sodium hydroxide, ion-exchange resins and two salts (sodium pyrophosphate and ethylenediamine hydrochloride), were used to extract humic materials from an organic soil. Extractabilities increased in the general order: salts < organic chelating agents < dipolar aprotic solvents < pyridine < ethylenediamine = sodium hydroxide, and the amounts of the soil organic matter extracted by the reagents in the series ranged from 13 to 63%. Gel chromatography techniques indicated that extracts in dipolar aprotic solvents were predominantly of intermediate and low molecular-weight values, and it is suggested that the more highly oxidised soil humic materials were extracted in these. The more efficient solvents extracted materials with a range (high—low) of molecular-weight values. Data from elemental analysis and from E.S.R. measurements indicated that ethylenediamine altered the chemical nature and the composition of extracts. Dipolar aprotics, by the same criteria, were found not to alter the humic extracts, and can be regarded as mild reagents for the extraction of a less representative (of the total) fraction of soil organic matter. Sodium hydroxide in solution, despite its oxidation effects, was the best of the reagents tested for isolating extracts which were representative of a wide range of soil humic substances.     

安徽省参考作物蒸散模型参数化

模型参数优化是准确估算参考作物蒸散(reference crop evapotranspiration,ET0)的关键问题之一。该研究基于安徽省81个地面气象站点1961—2011年逐日气象数据和合肥、武汉、南京、杭州和南昌5个辐射站1993—2011年的逐日辐射数据,评估日尺度的净长波辐射、气压和水汽压模型在安徽地区的适用性;并结合已有研究获得的最优逐日太阳辐射参数化估算模型,建立安徽省本地化逐日ET0模型的最优参数化方案,探讨模型参数优化对ET0估算的影响。结果表明:7种净长波辐射估算参数化方案中,邓根云法的精度最高,在安徽地区的适用性优于其他方案,建议作为安徽本地化方案使用;FAO56 Penman-Monteith公式中推荐的气压估算模型和基于实测平均气温和相对湿度估算水汽压的模型在安徽省基本适用,但该研究认为在资料能够获取的情况下直接使用实测值为最优。与基于实测资料计算的ET0相比,该研究建立的本地化最优模型估算的ET0在日、月和年尺度上的相对误差分别为15.5%、9.05%和6.12%,能较好地适用于安徽地区。FAO56 Penman-Monteith公式推荐的参数化方案由于高估了安徽地区的太阳辐射,低估了净长波辐射,导致其与基于实测资料计算的ET0值相比,在日、月和年尺度上高估ET0达40.0%以上,不推荐安徽地区直接使用。研究可为安徽省准确估算作物需水量、农业旱涝评估和合理调度水资源等提供依据。     

湿法烟气脱硫系统出口净烟气的温湿度变化特性

水汽相变技术与湿法烟气脱硫系统(wet flue gas desulfurization,WFGD)结合可有效控制细颗粒物排放,脱硫净烟气所能达到的过饱和度直接影响细颗粒物脱除效果,而烟气过饱和度取决于脱硫净烟气温湿度和水蒸气添加量。因而脱硫净烟气温湿度变化特性研究可为WFGD系统中应用水汽相变促进细颗粒物脱除提供依据,对于优化湿法脱硫操作条件、估算水蒸气添加量以及实现低能耗高效脱除细颗粒物具有重要意义。该文基于石灰石-石膏法脱硫工艺,试验考察了脱硫塔进口烟气温湿度、空塔气速、液气比、脱硫液温度等操作条件以及脱硫塔类型对脱硫净烟气温湿度的影响。结果表明,空塔气速由2.5 m/s提高至2.9 m/s可使脱硫净烟气相对湿度和绝对湿度分别由88%、82.2 g/kg先急剧降低至52%、57.3 g/kg,随后趋于相对平缓;液气比由5 L/m~3增至20 L/m~3有利于提高脱硫净烟气的相对湿度和绝对湿度,可分别由39%、46.4 g/kg提高至91%、84.3 g/kg,但相对湿度的增幅明显高于绝对湿度增幅;脱硫净烟气的相对湿度、绝对湿度及温度均随脱硫液温度升高而迅速提高,液气比为15 L/m3时,脱硫液温度由25℃升至60℃可使脱硫净烟气相对湿度、绝对湿度、温度分别由54%、25.9 g/kg、42℃提高至85%、98.5 g/kg、56℃,但脱硫液温度升至40℃后,脱硫净烟气的相对湿度趋于平缓;脱硫液温度保持不变时,提高塔进口烟气温度可使脱硫净烟气的绝对湿度和温度先明显增加,随后趋于平缓,而相对湿度随塔进口烟温提高稍有降低;并且不同的脱硫塔型对脱硫净烟气温湿度也有影响,液气比为5 L/m3时,经湍球塔、旋流板塔、喷淋塔脱硫后的净烟气相对湿度分别为90%、91%、44%。该研究结果为湿法烟气脱硫系统的应用提供参考。     

基于Dualex氮平衡指数测量仪的作物叶绿素含量估算模型

作物叶绿素含量的实时、无损及快速的监测,对及时掌握作物的胁迫状况、营养水平及环境适应性,进而对农田管理进行科学指导具有重要的意义。该研究论证是否可以通过Dualex氮平衡指数测量仪构建通用的叶绿素含量估算模型,以期实现叶绿素含量的快速及无损监测和估算。结果表明：1）Dualex估测叶绿素质量分数（Chl-M）和单位面积的叶绿素质量（Chl-S）具有较好的精度（决定系数R2分别为0.77和0.88）,与SPAD叶绿素仪的估算模型（R2分别为0.66和0.79）相比,模型精度更高;2）Dualex估测Chl-S的精度明显高于Dualex对Chl-M的估测精度,Dualex与Chl-M的关系需要考虑叶片厚度的影响,而Dualex与Chl-S的线性关系更加一致;3）构建的Chl-S通用模型的R2,均方根误差和标准均方根误差分别为0.88,4.80 mg/dm2和8.33%,模型的精度较高,并且通用模型的数据范围为12.2～105.6 mg/dm2,较大的数据范围适用于冬小麦和玉米各关键生育期Chl-S的估算。该研究为Dualex实现冬小麦和玉米叶绿素含量监测和估算提供校准模型,为及时了解作物养分状况及作物营养诊断提供了参考。     

Video image method compared to a hand method for determining root lengths

Root lengths are much more sensitive to treatment effects than root weights. Therefore, root length measurements are essential to research concerning rhizosphere effects on plant roots. A video image analyzer method was compared to a hand method for determining the root length of sorghum [Sorghum bicolor (L.) Moench] seedlings resulting from nutrient solution experiments. Initial root lengths were not significantly different, thus only final lengths were recorded. T‐tests for differences between hand and instrument values showed that although absolute values were often significantly different, there were much fewer significant differences in normalized data. Normalized instrumental root length data were well correlated with hand data (r > 0.96) and slopes of the regressions were equal to 1.00. Intercepts were higher than zero. Thus, although the normalized instrumental values for root length were somewhat higher than for hand root lengths, the instrument lengths were very precise in indicating relative differences among treatments.     

Phosphorus Fractions of Soils Treated with Phosphate Rock and Monocalcium Phosphate after Ryegrass Growth

Abstract

Excess EDDHA (ethylenediamine di(o‐hydroxyphenylacetic acid) ) had little effect on leaf concentrations of Fe in bush beans (Phaseolus vulgaris L. var Improved Tendergreen) when grown in nutrient solution at three pH values from 4.3 to 8.7. An excess of EDDHA 20 times that of Fe decreased yields slightly, but not as result of Fe deficiency. A resulting hypothesis is that chelated Fe can be transferred from chelating agent to uptake sites on roots without going in the ionic form through the solution around roots.     

低分子量有机酸对可变电荷土壤铝活化动力学的影响

从动力学角度研究了几种低分子量有机酸对2种酸性土壤中铝的活化和活化铝在土壤固/液相之间分配的影响。结果表明:对于络合能力弱的醋酸和乳酸,主要通过质子作用活化铝,且活化作用明显小于盐酸。而络合能力较强的苹果酸、草酸和柠檬酸,主要通过络合作用促进铝的释放,且这种作用随有机酸根阴离子络合能力的增强而增加。在氧化铁含量较高的砖红壤中,苹果酸、草酸和柠檬酸通过专性吸附增加土壤表面负电荷,从而增加土壤交换态铝;但在氧化铁含量较低的红壤中,草酸和柠檬酸主要通过形成可溶性铝络合物降低交换态铝。活化铝在土壤固/液相间的分配主要决定于溶液中有机阴离子与土壤固相表面对铝离子的竞争。醋酸和乳酸活化的铝主要以交换态铝存在;而草酸和柠檬酸活化的铝主要以有机酸-铝络合物存在于溶液中,特别是在氧化铁低的红壤中,这将促进铝在土壤-水体中的迁移。     

Effect of biodegradable chelating agents on heavy metals phytoextraction with Mirabilis jalapa and on its associated bacteria

The application of chelating agents can be associated with phytoremediation in order to reduce the time required for remediation of heavy metal contaminated soils. The present work has the purpose to test the use of easily biodegradable chelating agents in the assisted phytoextraction process and to evaluate their effect on soil and Mirabilis jalapa plant associated bacteria. Two easily biodegradable chelating agents were used (EDDS and MGDA) in two different dosages (4 and 8 mmol/kg of soil). Metal concentration in soil solution, in leaves and in leachate were determined during the phytoextraction process, while at the end of the experiment metal concentration was evaluated separately in roots, stalk and leaves. In untreated reactors Zn and Pb were accumulated in the roots, but only Zn was translocated to the shoots. Both chelating agents demonstrated to be very effective in Pb and Zn mobilization in soil solution. After chelate treatment, accumulation and translocation of the two metals was different: unaffected with regard to Zn and enhanced with regard to Pb. The chelating agents seem to have a positive influence on bacterial communities of bulk soil and rhizosphere by mitigating the selective pressure caused by Pb and Zn, whereas the endophytes are less affected.     

柑橘叶面积指数自动测量红外透射系统的开发与应用

针对间接方法测叶面积指数(leaf area index,LAI)一般不考虑单一孤立果树冠层、叶片相互重叠因素及图像处理速度低的缺点,通过正交试验确定单一孤立柑橘LAI测量的主要影响因素为重叠叶片数(α<0.05),建立等间隙模型解决有间隙的重叠的影响问题,采用三维行走机构实现自动采集,应用LabVIEW平台开发一个基于红外透射的柑橘LAI自动测量系统。系统硬件由控制模块与行走机构、信号调理模块、数据采集卡和PC机组成,其标定LAI相对误差绝对值平均为11.11%。在行走机构按照S形路线,自动进行扫描、定位采集的方式下,系统对华南农业大学工程学院柑橘园45棵橘树测量,以直接法和采样法计算的LAI值作为真实值,相对误差绝对值的平均为31.4%;参照对比的Win SCANOPY Pro植物冠层分析系统专业版测量值与真实值比较,相对误差绝对值的平均为29.8%。两系统误差接近,表明本系统可满足柑橘LAI测量试验要求。     

Melanoidins from coffee infusions. Fractionation, chemical characterization, and effect of the degree of roast

A method involving fractionation in ethanol aqueous solutions, anion exchange chromatography, and immobilized copper chelating chromatography was developed to obtain high molecular weight anionic melanoidin populations from coffee infusions. Six anionic fractions with different physicochemical properties (ethanol solubility and chelating ability) and chemical composition regarding carbohydrate as well as protein nature and content were isolated. Fractions with similar chemical composition were obtained for light-, medium-, and dark-roasted coffee infusions. These melanoidin fractions accounted for 30-33% of the cold-water soluble high molecular weight material, independently of the degree of roast in coffee. The nature and abundance of the different polysaccharides in each fraction were dependent on their ethanol solubility. The 50% ethanol insoluble melanoidin populations contained mostly galactomannan-like carbohydrates, and the fractions obtained with 75% ethanol contained mostly arabinogalactan-like carbohydrates. The melanoidin populations with chelating properties presented significantly lower carbohydrate content and, from these, the 75% ethanol soluble fractions were almost devoid of carbohydrate material. The results obtained suggest that the chelating ability of these coffee melanoidins is modulated by their carbohydrates.     

不同土壤温度和容重下微咸水上升毛管水

为探索西北干旱地区不同质地土壤以及土壤温度和容重对微咸水上升毛管水运动特性的影响,通过模拟和室内试验,研究了土壤温度和容重对砂壤土和粉壤土上升毛管水运动特性的影响。分别建立了地下水补给量与土壤温度和容重之间的模型,毛管水上升高度与土壤温度和容重之间的模型,其中地下水补给量模型,砂壤土的绝对系数均达到0.96以上,计算值与模拟值最大相对偏差小于10.9%,粉壤土的绝对系数均达到0.96以上,计算值与模拟值最大相对偏差小于8.4%,毛管水上升高度模型,砂壤土的绝对系数均达到0.996以上,计算值与模拟值最大相对偏差小于8.37%,粉壤土的绝对系数均达到0.997以上,计算值与模拟值最大相对偏差小于11.9%,模型拟合程度较好,所建立方程符合实际情况。砂壤土盐分主要聚集在距地表1.70~2.20 m处,粉壤土盐分主要聚集在距地表0.3~1.3 m处,砂壤土土壤盐分分布受土壤温度和容重的影响较显著,而粉壤土土壤盐分分布受其影响并不显著。该研究结果可为盐碱化地区水盐管理提供科学依据。     

双层膜锑pH电极及其测试仪的研制与应用

pH值是工农业生产、医学检验和环境保护等领域十分重要的化学参数。针对目前农业生产中玻璃电极及其测试仪存在影响因素多、测量误差大等不足,依据锑pH电极敏感机理和全氟磺酸树脂(Nafion)修饰膜选择性透过特性,应用16位低功耗微控制器(单片机)凌阳SPCE061A研制一种经双层膜修饰锑pH测试仪。通过对电极性能测定试验和pH测试仪土壤pH检测试验,结果表明:在pH值为1～12,电极输出电势E与pH值线性度好,相关系数R2为0.997,具有响应快、回差小、抗干扰能力强等优点;pH测试仪在测定土壤浑浊液pH值时,误差绝对值小于0.075,相对误差绝对值小于1.342%,在工农业生产中具有广阔应用前景。     

Equilibrium Isotherm Studies for the Sorption of Divalent Metal Ions onto Peat: Copper,Nickel and Lead Single Component Systems

The sorption of three divalent metal ions — copper, nickel and lead — from aqueous solution onto peat in single component systems has been studied and the equilibrium isotherms determined. The experimental data have been analysed using the Langmuir, Freundlich, Redlich-Peterson, Toth, Temkin, Dubinin-Radushkevich and Sips isotherm models. In order to determine the best fit isotherm for each system, six error analysis methods were used to evaluate the data: the coefficient of determination, the sum of the errors squared, a hybrid error function, Marquardt's percent standard deviation, the average relative error and the sum of absolute errors. The error values demonstrated that the Sips equation provided the best model for the three sets of experimental data overall.     

气象要素时间分辨率对参考作物蒸散估算的影响

参考作物蒸散(reference evapotranspiration,ET0)的准确估算是农业水资源合理利用的重要环节。为了明确气象要素不同时间分辨率对参考作物蒸散估算的影响,该文基于寿县国家气候观象台2007-2013年观测资料,将1min时间分辨率数据平均值作为真实值,分析了10、20、30、40、60 min、4次/d(02:00、08:00、14:00、20:00)和3次/d(08:00、14:00、20:00)这7种不同时间分辨率对逐日气温、风速、太阳辐射、相对湿度和日、月及年参考作物蒸散(ET0)估算的误差情况。结果表明:ET0和气象要素的误差整体上随时间分辨率降低而增大。4个气象因子中,日平均风速估算受时间分辨率变化的影响最显著,误差最大;其次是太阳辐射。逐日ET0估算在7种时间分辨率的平均绝对相对误差(mean absolute relative error,MAPE)依次为0.53%、1.01%、1.38%、1.72%、2.46%、4.72%和6.14%,表明10至60min时间分辨率的估算效果相较3次/d和4次/d有明显改善。10至40 min的绝对误差超过95%都在-0.20~0.20 mm/d区间内,误差较小且集中度高;太阳辐射时间分辨率变化对ET0估算误差贡献最大,其次是风速,这主要是由于两个要素本身对分辨率较敏感且分别是ET0辐射项和动力项的主要组成因子。时间分辨率的变化对累计后长时间尺度ET0的影响较小,月和年ET0的误差明显小于逐日ET0,月ET0在7种时间分辨率的MAPE值依次为0.13%、0.21%、0.27%、0.40%、0.50%、1.18%和1.48%;各年ET0相对误差(relative error,PE)的绝对值多数均小于0.50%。     

Rainfall acidity in northern Britain — Exploring the data

Daily rainfall pH measurements from a site in south Scotland were found to have a bimodal distribution. Similarly, monthly data from a network of bulk rain collectors in northern Britain gave bimodal distributions. Sites with an annual average pH of 4.7 had occasional monthly pH values of pH 4 or less. The annual average pH was a poor indicator of the frequency of such acidic periods, and may not be a good indicator of potential effects. Data from a daily and monthly collector at the same site are compared and the discrepancies discussed. Daily pH measurements gave significantly greater acidities than monthly pH measurements, but these differences were small for months with rain-weighted pH < 5.     

Effects of genotype and environment on the antioxidant properties of hard winter wheat bran

Recent consumer interest in controlling and preventing chronic diseases through improved diet has promoted research on the bioactive components of agricultural products. Wheat is an important agricultural and dietary commodity worldwide with known antioxidant properties concentrated mostly in the bran fraction. The objective of this study was to determine the relative contributions of genotype (G) and growing environment (E) to hard winter wheat bran antioxidant properties, as well as correlations of these properties to growing conditions. Bran samples of 20 hard winter wheat varieties grown in two locations were examined for their free radical scavenging capacities against DPPH, ABTS cation, peroxyl (ORAC), and superoxide anion radicals and chelating properties, as well as their total phenolics and phenolic acid compositions. Results showed significant differences for all antioxidant properties tested and multiple significant correlations between these properties. A factorial designed analysis of variance for these data and pooled previously published data showed similar results for four of the six antioxidant properties, indicating that G effects were considerably larger than E effects for chelating capacity and DPPH radical scavenging properties, whereas E was much stronger than G for ABTS cation radical scavenging capacity and total phenolics, although small interaction effects (GxE) were significant for all antioxidant properties analyzed. Results also showed significant correlations between temperature stress or solar radiation and some antioxidant properties. These results indicate that each antioxidant property of hard winter wheat bran is influenced differently by genotype and growing conditions.     

模拟雨水连续淋洗下土壤化学性状动态变化特征的研究

本文从研究正常降雨对土壤性状的影响出发,进行了模拟雨水对酸性紫色土和石灰性紫色土的连续淋洗试验。试验结果表明在模拟雨水连续淋洗150天的过程中,土壤中钙、镁、钾、钠等盐基离子几乎都显示出一定程度的净淋溶特征,土壤中各盐基离子的动力学变化特征几乎都与抛物线扩散方程吻合。淋洗结束后土壤酸度未发生显著变化,但两种土壤CEC值均有所降低。     

Interaction of iron chelates with several soil materials and with a soil standard

Frequently the effectiveness of iron (Fe) chelates is low because they can be retained or destroyed by soil materials. The high cost of these Fe fertilizers makes it necessary to study soil material reaction with Fe chelates. Commercial Fe chelates with EDTA, EDDHA, and EDDHMA as ligands and their standards, prepared in the laboratory, were shaken for one hour with various soil materials [amorphous Fe(III) oxide, acid peat, calcium (Ca)‐montmorillonite and calcium carbonate (CaCO₃)] and with a soil standard made in the laboratory. After agitation, the chelate‐soil mixtures were filtered and the micronutrients and chelated Fe that remained in solution were determined. Among the soil materials used, amorphous Fe(III) oxide and acid peat had the greatest affect on the amount of chelated Fe remaining in solution. The type of chelating agent was the next major factor that affected the availability of soluble Fe following reaction with the soil materials. Another factor was the commercial formulation of the Fe chelates. The chelates comprised of EDDHA or EDDHMA maintained the highest percentages of chelated Fe in solution after interaction with the solid phases, except for the acid peat. The last soil material, acid peat, retained more chelated Fe for the Fe chelates with EDDHA or EDDHMA than with EDTA as the chelating agent. The commercial Fe‐EDDHA chelates had greater losses of chelated Fe than their standard after interaction with all the solid phases. The commercial Fe‐EDDHA chelate (Sequestrene) and the commercial Fe‐EDDHMA chelate (Hampirón) solubilized the highest amount of copper (Cu) from soil standard. This was attributed to the presence of by‐products in the commercial formulations since the Fe‐EDDHA standard did not have Cu in solution after the interaction. Therefore, the commercial Fe chelate by‐products are able to form Cu‐complexes which could affect chelated Fe and its availability to plants.