猪A型产气荚膜梭菌病研究

家畜A型产气荚膜梭菌病是我省首先发现的一种多种家畜共患的传染病。目前全国许多省市都有发生。由于该病往往没有任何前驱症状，突然发病死亡，在没有确定该病病原之前，人们通常称之家畜“猝死症”。我省该病最初见于1985年河南周口市某县，当时呈零星散发，患病家畜以犊牛和仔猪为主。近几年来，仔猪对该病的感染率呈上升趋势，为了有效控制该病，笔者曾系统参与了我省A型产气荚膜梭菌病的研究，现将其研究结果报告于后。     

Hemorrhagic enteritis caused by Clostridium perfringens type C in a foal

A 4-day-old foal died with bloody diarrhea. Using a mouse neutralization test, Clostridium perfringens type C was isolated from intestinal contents, and alpha and beta toxins were identified. About 4 m of the jejunum had severe necrohemorrhagic enteritis. Microscopically, large, rod-shaped, gram-positive bacteria were seen on necrotic intestinal villi by use of Brown and Hopp's stains.     

A型产气荚膜梭菌致奶牛腹泻的诊断与防制

20 0 1年 8～ 9月 ,江苏某奶牛场奶牛陆续发生腹泻 ,采用氟哌酸治疗一段时间之后 ,收效甚微 ,因而怀疑为由产气荚膜梭菌所致的腹泻 ,遂对其进行了实验室诊断和药敏试验 ,并采取了相应的措施 ,取得了较好的效果。现将内容报告如下。1　病料采集与细菌分离从发病奶牛群中随机采集病牛新鲜排粪 1 2份 ,用灭菌生理盐水做 1∶ 1稀释后 ,用酪蛋白 -硫酸亚铁 -环丝氨酸琼脂 ( TSC,购自德国 Merck公司 )平板划线分离 ,置厌氧培养罐 (购自 Merck公司 ) 37℃培养 2 4 h,TSC琼脂平板上长出多量疑似菌落 ,菌落形态为圆形、突起、边缘整齐、表面湿润…     

Severe heart muscle degeneration caused by Clostridium perfringens type A in camel calves (Camelus dromedarius).

Clostridium perfringens type A was isolated from different organs and intestines from three to five weeks old camel calves which have died from heart muscle necrosis. No other bacterial pathogens were isolated. Virus isolation on two different cell lines including a fetal camel skin were also negative. Mice which were injected with bacteria free filtrates prepared from intestinal contents of necropsied camel calves died after one to six hours demonstrating the presence of clostridial toxins. Our findings suggest that the cardiac muscle necrosis is caused by Clostridium perfringens type A toxins.     

Predisposing factors in enterotoxemias of camels (Camelus dromedarius) caused by Clostridium perfringens type A.

C. perfringens type A was isolated from different organs and intestines from breeding and racing camels which died from peracute and acute enterotoxemias in two separate outbreaks. Pathological changes in the digestive tract were mild in breeding camels, and severe in racing camels. A polyvalent clostridial antiserum of bovine origin given intravenously had a life-saving effect on breeding camels, but not on racing camels. In the two outbreaks, fifty percent of the breeding camels were suffering from an acute Trypanosoma evansi infection, and 25% of the racing camels had developed a salmonellosis. It is suggested that both infections played an important role as predisposing factors for the outbreak of C. perfringens enterotoxemias.     

仔猪产气荚膜梭菌肠毒血症病原诊断及疫苗研究Ⅲ.7株A型产气荚膜梭菌菌株的产毒素试验

本试验对7株A型产气荚膜梭菌菌株在不同培养基、不同培养温度和不同培养时间的产毒力进行了试验。结果表明，培养基的种类和培养时间对菌株的产毒素能力影响较大，而培养温度则影响较小。所试菌株中C57-1菌株的产毒素能力最强，是制备疫苗的最佳菌株。     

仔猪产气荚膜梭菌肠毒血症病原诊断及疫苗研究IV．肠毒血症C型疫苗生产用菌种的确定

试验证明用单一菌株C59-2生产C型产气荚膜梭菌肠毒血症干粉灭活疫苗,其效力可以达到《规程》要求,并且简化了生产工艺,降低了生产成本,提高了生产效率。疫苗免疫的兔血清可分别中和原来3个制苗用菌株所产生的外毒素。