Interactive effects of salinity and iron deficiency on different rice genotypes

Salinity adversely affects plant growth, photosynthesis, and availability of nutrients including iron. Rice (Oryza sativa L.) is susceptible to soil salinity and highly prone to iron (Fe) deficiency due to lower release of Fe‐chelating compounds under saline conditions. In order to investigate the effects of salinity and low iron supply on growth, photosynthesis, and ionic composition of five rice genotypes (KS‐282, Basmati Pak, Shaheen Basmati, KSK‐434 and 99417), a solution culture experiment was conducted with four treatments (control, 50 mM NaCl, Fe‐deficient, and 50 mM NaCl + Fe‐deficient). Salinity and Fe deficiency reduced shoot and root growth, photosynthetic and transpiration rates, chlorophyll concentration, and stomatal conductance. The reduction in all these parameters was more in the interactive treatment of salinity and low Fe supply. Moreover, a significant increase in shoot and root Na⁺ with corresponding decrease in K⁺ and Fe concentrations was also observed in the combined salinity and Fe‐deficiency treatment. Among the tested genotypes, Basmati Pak was the most sensitive genotype both under salt stress and Fe deficiency. The genotype KS‐282 performed better than other genotypes under salinity stress alone, whereas Shaheen Basmati was the best genotype under Fe deficiency in terms of all the studied parameters.     

缺铁胁迫对草莓幼苗光合特性及细胞器铁含量的影响

为了探讨缺铁胁迫对草莓（Fragaria ananassa Duch.）幼苗的光合特性及细胞器铁含量的影响,本研究选取4个草莓品种（红颜、 章姬、 甜查理、 童子一号）幼苗,采用溶液培养方法,设置Fe（Ⅱ）-EDTA浓度为0 mol/L、 110-4 mol/L两组处理,分别于处理后0、 4、 8、 12、 16 d对其叶绿素含量（SPAD）、 光合速率（Pn）、 叶绿体铁含量、 根系线粒体铁含量以及叶片铁含量、 根系铁含量、 生物量进行分析。结果表明,缺铁胁迫显著降低草莓幼苗叶绿素含量、 光合速率、 叶绿体铁含量、 叶片铁含量、 根系铁含量、 生物量,并且不同品种间差异达显著水平（P0.05）;缺铁胁迫对根系细胞线粒体铁含量影响较小。草莓的叶绿体铁含量与叶片铁含量、 叶片净光合速率和生物量呈极显著正相关（r=0.93**, r=0.87**, r= 0.72**）, 根系线粒体铁含量与叶片铁含量、 叶片净光合速率和生物量呈极显著正相关或显著正相关（r= 0.83**, r= 0.72**, r= 0.52*）。本试验条件下,供试草莓品种红颜受缺铁胁迫的影响大于其他3个品种。     

膜下滴灌水稻基因型耐缺铁性评价

【目的】新疆生产建设兵团采用膜下滴灌技术后,水稻缺铁黄化现象较为严重,研究该条件下水稻对缺铁反应的基因型差异,可为耐缺铁性水稻基因型的筛选和分类提供可靠的理论依据。 【方法】以六个水稻基因型为研究材料,采用膜下滴灌技术管理。在水稻幼苗期、分蘖期和成熟期随机采样,测定不同基因型水稻的分蘖数、生物量、产量以及铁含量,利用隶属函数分析和聚类分析研究了水稻在全生育期对缺铁胁迫反应的基因型差异。 【结果】水稻在幼苗期、分蘖期和成熟期对缺铁的反应存在基因型差异。在幼苗期水稻基因型 T-04 和 T-05 的叶片活性铁含量显著低于其他基因型;T-201 在幼苗期叶片活性铁、地上部铁的分配,分蘖期叶片铁含量和地上部干物质均较高;在分蘖期 T-04 铁的转移能力最小,但是 T-04 的分蘖数较高;在成熟期 T-04 的有效分蘖与其他基因型差异不显著,T-04 的产量处于中等水平,但是其籽粒铁的收获指数低于其他基因型水稻。聚类分析显示耐缺铁水稻基因型 T-43 的各指标高于其他基因型。 【结论】在水稻的幼苗期和分蘖期叶片铁的有效利用和自身铁的转移保证了水稻的生长和较高有效穗数,根据全生育期水稻铁营养效率和产量的基因型差异初步确定 T-43 耐缺铁能力较强,T-04 为对缺铁敏感的水稻基因型。     

Clay mineralogical and ammonium fixation characteristics of some tropical upland rice soils

Abstract

In view of the agronomic and economic significance of NH₄ fixation in soils, an attempt has been made to relate this to the most reactive mineral constituents of soils ‐ the clay minerals, under the temperature‐moisture regimes normal to tropical upland rice soils. Laboratory fixation study was done with NH₄, concentrations similar to those common in soils upon N fertilization, and under alternate wetting and drying at ambient temperatures rather than at 100°C as in many published studies.

Results of the investigation show that soil clays with dominant vermiculite and montmorillonite fix the greatest proportion of applied NH₄ (94 and 91%), followed by beidellite (72%) and x‐ray amorphous (45–64%) clays. Fixation is negligible (10%) in the clay with mineral suite consisting of hydrous mica, halloysite, and chlorite. Crystallinity of minerals seems to influence NH₄ fixation appreciably.     

不同铁形态对水稻根表铁膜及铁吸收的影响

通过溶液培养试验研究了FeCl2?4H2O和FeCl3?6H2O对水稻根表铁膜数量及铁吸收的影响。结果表明,FeCl2处理时水稻根表铁膜浓度是FeCl3处理的197%～233%。利用EDTA-BPDS对铁膜形态分析看出,根表铁膜中Fe3+占85%～92%,Fe2+占8%～15%。水稻天优998根表铁膜数量显著高于培杂泰丰,其铁吸收是培杂泰丰的115%～138%。两种铁形态处理明显提高水稻的根系活力,其中,FeCl2处理时水稻根系活力增加24%～69%,FeCl3为16%～54%。FeCl2处理时水稻根系SOD、POD和CAT活性分别增加11%～32%、15%～30%和30%～31%,但FeCl3处理没有明显影响。上述结果表明一定浓度铁处理明显增加水稻根表铁浓度和铁吸收;与FeCl3处理相比,FeCl2处理能提高根系抗氧化酶活性,增加水稻的铁吸收和根表铁膜数量。     

华南地区覆膜旱种稻田甲烷排放及其与土壤水分和温度的关系

采用静态箱法和田间小区试验,研究了常规稻田和覆膜旱种稻田水稻全生育期CH4的排放规律,探讨了温度和水分与稻田CH4排放的关系。结果表明:覆膜旱种稻田的甲烷排放量明显低于常规水田的排放量,常规水田的甲烷累计排放通量为20.38g/m2,覆膜旱种稻田为2.46g/m2,水稻覆膜旱种后甲烷排放量降低了88%。常规水田CH4排放峰期持续了35d,覆膜旱种稻田CH4排放峰期为25d,两者在CH4排放高峰期的排放量分别占整个生育期累计排放量的72%和97%。覆膜旱作稻田CH4排放量降低,主要表现在最大排放峰值降低和排放峰持续时间缩短。土壤温度(5cm处)和水分与水稻生育期稻田甲烷的排放有显著正相关。CH4排放通量大于1.0mg·m-2·h-1主要集中在土壤质量含水率高于36.25%的区域,在土壤质量含水率小于36.25%时,常规稻田和覆膜旱种稻田都只有少量CH4排放。     

Physiological basis of iron chlorosis tolerance in rice (Oryza sativa) in relation to the root exudation capacity

Micronutrient deficiency in cultivable soil, particularly that of iron (Fe) and zinc (Zn), is a major productivity constraint in the world. Low Fe availability due to the low solubility of the oxidized ferric forms is a challenge. An experiment was, thus, executed to assess the performance of eight genetically diverse rice genotypes on Fe-sufficient (100 µM) and Fe-deficient (1 µM) nutrient solution, and their ability to recover from Fe deficiency was measured. Fe efficiency under Fe deficiency in terms of biomass production showed a significant positive correlation with the root release of phytosiderophore (PS) (R² = 0.62*). This study shows that the Fe deficiency tolerance of Pusa 33 was related to both a high release of PS by the root and an efficient translocation of Fe from the root to the shoot as the Fe–PS complex, which could be useful for improving the Fe nutrition of rice particularly under aerobic conditions.     

Characterisation of globally diverse blast-resistant upland rice (Oryza sativa L.) germplasms based on morpho-physiological and yield attributes

Upland rice is a valuable base population for protecting against genetic erosion and broadening the gene pool of rice germplasms, and rice is also the staple food of more than 100 million people across the world. Therefore, an experiment was conducted to elucidate the performances of 27 globally diverse blast-resistant upland rice genotypes in terms of morphological, physiological and yield trait characteristics and to determine the correlations between and assess the diversity among these characteristics. All of the genotypes were evaluated in a randomised complete block design with three replications. Analysis of variance revealed 18 traits with significant to highly significant variations among themselves. The BRRI dhan42, BRRI dhan43, Ja Loy, C, Choke Tang and Chirikata 2 genotypes matured early, and the Chirikata 2, C, Choke Tang, BRRI dhan43 and Padi Beleong genotypes were best in terms of yield. The greatest heritability was recorded for the numbers of filled grains/panicle and the yield/plant, which demonstrated that these traits can be successfully transferred to the offspring if selection for these characteristics is performed in a hybridisation programme. The 27 genotypes were grouped into six clusters based on the contributing morphological, physiological and yield characteristics. The first four principal components accounted for 40, 59, 70 and 79% of the total variation, respectively, which indicated abundant genetic differences between these genotypes. The Chirikata 2, C, Choke Tang and BRRI dhan43 were selected as the best genotypes and could be used as valuable resources for high-yielding, early maturing, blast-resistant cultivars and parental sources for hybridisation programmes for challenging environments in the future.     

Effect of dual applied phosphorus and gypsum on wheat forage and grain yield

Winter wheat (Triticum aestivum L.) production on acid soils can be greatly affected by reduced phosphorus (P) availability. At low pH (below 5.5), iron (Fe) and aluminum (Al) react with P to form highly insoluble compounds that severely reduce the amount of plant available P. Previous research suggested that supersaturating localized P fertilizer bands with respect to Ca²⁺ could induce precipitation of applied P as dicalcium phosphate (DCP) or dicalcium phosphate dihydrate (DCPD) which would slowly become plant available with time. The objective of this study was to determine the effect of dual‐band applications of P and gypsum on winter wheat forage and grain yield. Methods of application included P and gypsum banded with the seed, P and gypsum broadcast, and P banded and gypsum broadcast at rates of 29 and 58 kg P ha^‐1 and 22 and 44 kg S as gypsum ha^‐1. Sources of P included diammonium phosphate (DAP; 18–20–0) and triple superphosphate (TSP; 0–20–0). Grain and forage yields increased when P was applied. Dual‐band applications of P and gypsum increased wheat grain and forage yields compared to P banded without gypsum, and P banded and gypsum broadcast. When DAP was the P source, the N‐P band reduced yields compared to P banded alone or the N‐P‐gypsum band. This suggests that gypsum should be included in the band for maximum benefit. Precipitation of DCPD and DCP may have taken place within the dual P‐gypsum band, reducing fertilizer P fixed as Fe or Al hydroxides thus increasing long‐term P availability for winter wheat forage and grain production on acid soils.     

Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco

Barley ( Hordeum vulgare L.) nicotianamine synthase gene ( HvNAS1 ) expression in barley is strongly induced by Fe deficiency in the roots and rice ( Oryza sativa L.) nicotianamine synthase gene ( OsNAS1 ) expression in rice is induced by Fe deficiency both in the roots and in the shoots. In dicots, NAS genes are not strongly induced by Fe deficiency, and they function to maintain Fe homeostasis. Rice OsNAS1promoter::GUS or barley HvNAS1promoter::GUS was introduced into tobacco ( Nicotiana tabacum L.) and tissue specificities and systemic regulation of their expression were compared. A split-root experiment revealed that the HvNAS1 promoter exhibited functions similar to those of Fe-acquisition-related genes in tobacco roots, suggesting that this promoter responded to certain Fe-deficiency systemic signals and to the Fe concentration in the rhizosphere. The HvNAS1 promoter might harbor a type of universal system of gene expression for Fe acquisition. However, the OsNAS1 promoter did not respond to local application of Fe to the roots and induced GUS activities in mature leaves in response to Fe deficiency. This promoter might possess numerous types of cis -acting sequences that are involved in Fe metabolism.     

镉胁迫对两个水稻品种幼苗光合参数、可溶性糖和植株生长的影响

以较耐镉(武育粳3号,WYJ)和镉敏感(汕优63,SY63)的2个不同耐性水稻品种为材料,采用溶液培养试验,研究了镉处理(Cd.1.0和5.0mol/L)对水稻幼苗光合作用、可溶性糖和生长的影响。结果表明,镉胁迫下,水稻幼苗的光合作用显著地受到抑制。与不加镉的对照相比,随镉水平的提高水稻幼苗净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、气孔限制值(Ls)逐步下降,尤其是在Cd.5.0mol/L处理下的植株,下降幅度武育粳3号显著小于汕优63。蒸腾效率(Te)也随镉浓度的增加而降低,但变化与净光合速率等不同,武育粳3号的下降速率要大于汕优63的。加镉处理使2个水稻品种幼苗叶片可溶性糖含量均显著升高,而叶绿素含量仅汕优63在Cd.5.0mol/L处理下与对照差异显著。加镉处理使汕优63根系生长(根长)显著受到抑制,而株高仅在Cd.5.0mol/L时显著降低。武育粳3号生长与对照差异不显著。本研究结果表明,随着镉处理水平的提高,镉对水稻幼苗的毒害作用增加,但是水稻对镉毒害的响应存在明显的基因型差异。     

Effects of mycorrhizal inoculation of upland rice on uptake kinetics of arsenate and arsenite

We assessed the effect of mycorrhizal inoculation on short‐term uptake kinetics of arsenate and arsenite by excised roots of upland rice (Oryza sativa L. cv. Zhonghan 221). A concentration of 0.01–0.05 mM arsenic (As) differentially affected the influx rates of both arsenate and arsenite into rice roots non‐inoculated or inoculated with Glomus mosseae and G. versiforme. While V_max for arsenate uptake by non‐mycorrhizal roots was 1.02 µmol g^?1 fresh weight h^?1, it was reduced by a factor of 2.4 for mycorrhizal roots (about 0.42 µmol g^?1 fresh weight h^?1) in the high‐affinity uptake system. However, at high concentrations of 0.5–2.5 mM As only G. versiforme was able to reduce As influx. The results show that mycorrhizal effects on As uptake of upland rice are both concentration and species‐specific.     

播种方式对稻茬小麦生长发育及产量建成的影响

目前稻茬麦机播面积不断扩大,为研究播种方式转变对小麦播种出苗、生长发育与产量建成的影响,2009-2012年,在成都平原稻茬麦区开展撒播(免耕+人工撒种+人工覆盖稻草)与机播(免耕+稻草粉碎覆盖+2BMFDC-6型播种机播种)比较试验。结果表明,机播处理的播种效率、出苗率、麦苗均匀度,以及中前期的个体与群体质量均显著高于撒播处理。但到了生育后期,机播小麦的个体与群体质量反而不及撒播小麦,进而影响穗部性状。机播小麦开花期干物质积累量和叶面积指数的年均值较撒播小麦低1.8%、8.9%,成熟期单穗结实小穗数和穗粒数较撒播处理低4.2%、3.5%,但千粒质量较撒播高4.9%,籽粒产量则基本相当。机播小麦开花期耕层土壤的速效氮含量较撒播处理低7.8%,植株全氮含量低19.4%。增施氮肥后,机播小麦个体和群体质量得到改善,增产趋势明显。表明,2BMFDC-6型机播有利于提高稻茬小麦播种效率和质量,但需要适当提高施氮水平以提高中后期个体与群体质量,进而实现高产。研究结果可为稻茬小麦高产高效栽培技术的熟化完善提供理论和技术依据。     

磁化水灌溉促进水稻生长发育提高产量和品质

为明确磁化水灌溉对水稻生长发育、产量形成和品质的影响及其机理,该研究以杂交籼稻中浙优1号和杂交粳稻甬优9号为试验品种,于2012-2013年进行了磁化水(F型变频磁化水处理器的磁场强度为0.2 T)和普通水(CK)灌溉大田试验。结果表明,与CK相比,磁化水灌溉显著增加水稻的有效穗(增幅4.0%~7.9%)、结实率(3.9%~8.7%)和产量(增幅5.2%~9.3%)。2013年,磁化水灌溉提高了水稻的低位分蘖发生率(增幅9.4%~21.5%),增加了孕穗期、灌浆期倒三叶的SPAD值(增幅2.4%~7.8%)和干物质积累量(增幅8.7%~18.8%);磁化水灌溉显著改善稻米品质,中浙优1号和甬优9号的的垩白粒率分别降低了13.3%和12.0%,垩白度分别降低了11.4%和7.7%,胶稠度分别提高了6.0和4.0 mm,碱消值提高了4.3%和4.8%。该结果为磁化水在水稻生产上的大面积应用提供了科学依据。     

覆膜旱种降低水、陆稻锰素吸收提高锰素利用效率

揭示覆膜与裸地旱种对水、陆稻锰(Mn)素吸收利用的差异。以陆稻中旱3号和水稻武香粳99-8为供试材料,以传统淹水种植方式为对照,设置覆膜和裸地2种旱种方式,研究了覆膜旱种和裸地旱种对Mn素吸收利用的影响。结果表明,陆稻中旱3号覆膜旱种的产量较对照显著降低9.0%,而水稻覆膜旱种的产量较对照无显著差异,陆稻和水稻裸地旱种的产量分别较水种显著降低11.7%和8.0%。旱种使稻株的含Mn量、稻米中的含Mn量和Mn素累积量均减少,而Mn素物质生产效率、Mn素籽粒生产效率和Mn素收获指数均增加,旱种还使Mn素在稻株穗部分配比例增加,在叶片和茎鞘中的分配比例表现不一。与武香粳99-8相比,中旱3号生育后期稻株含Mn量提高13.1%～20.6%,Mn素累积量较少5.2%～9.6%,Mn素物质生产效率和Mn素籽粒生产效率降低,Mn素收获指数增加。水种时中旱3号稻米中Mn含量明显低于武香粳99-8,而旱种时则表现相反。表明旱种稻株对Mn素吸收利用因种植方式和品种类型不同而有较大差异。     

机械旱直播方式促进水稻生长发育提高产量

为了明确机械旱直播模式下水稻的产量形成机制,以杂交籼稻(F优498)和常规粳稻(徐稻4号)为试材,通过一次浅旋耕机械旱直播(B1)、"调墒、两旋"机械旱直播(B2)2种机械旱直播处理,并以人工撒播(B3)处理为对照,研究其对直播水稻产量形成的影响及其生理特性。结果表明,品种与直播处理对水稻主要生育时期干物质累积、叶面积指数(leaf area index,LAI)、结实期剑叶净光合速率与保护酶活性、根系伤流强度、茎秆抗倒伏特性及产量均存在显著影响。不同直播处理下,F优498在产量及生长发育特性各指标上均显著高于徐稻4号。与B3相比,同一品种下机械旱直播各处理均能优化群体茎蘖数,提高成穗率,保证齐穗期适宜的LAI,提高了高效叶面积率,并有利于提高结实期群体光合产物的积累、剑叶净光合速率与保护酶活性、根系伤流量及茎秆抗倒伏能力,并在保证一定数量有效穗的前提下,显著提高穗粒数、结实率及千粒质量,是机械旱直播水稻高产的重要原因。不同机械旱直播处理下,B2比B1处理能进一步提高机直播水稻齐穗至成熟期干物质累积并延缓LAI的衰减、增强根系活力及茎秆的抗倒能力,提高了结实率及千粒质量,最终促进了产量的提高,为最佳的机械旱直播方式。     

施肥模式对双季稻产量、养分吸收及经济效益的影响

水旱轮作田间定位试验条件下研究了连续4年（20052008）不同施肥模式对双季稻产量、养分吸收及经济效益的影响。结果表明,氮磷钾肥配施能显著提高水稻产量,长期有机无机肥配施增产效果明显。定位4年后,有机无机肥配施处理早稻和晚稻平均产量分别比未施肥处理增产135%和130%。随着产量的增加,稻草与稻谷中氮、磷和钾养分吸收量呈增大的趋势,表明水稻植株养分吸收量与稻谷产量呈显著正相关。不同施肥模式显著影响水稻稻草、稻谷中的氮、磷、钾养分含量。氮磷钾肥配施处理,特别是有机无机肥配施处理稻谷的养分吸收量最高,在土壤中的氮、磷盈余也最少。肥料配施虽增加了支出,但提高了经济产出,其纯收益相对较高,以有机无机肥配施处理的纯收益最高。双季稻生产实践中,有机无机肥配施模式值得推荐且需合理增施磷、钾肥。     

Responses of aerobically grown iron chlorosis tolerant and susceptible rice (Oryza sativa L.) genotypes to soil iron management in an Inceptisol

Iron deficiency is a serious nutritional disorder in aerobic rice, causing chlorosis, poor yields and reduced grain nutritional quality. The problem can be managed by complementing the use of Fe-efficient plant type with a suitable Fe management strategy. In the present paper, we report the effect of eight iron management practices to resolve the problem of iron (Fe) chlorosis through the use of an iron deficiency tolerant (IDTR) and iron deficiency susceptible (IDSR) rice genotype, i.e. Pusa 33 and ADT 39, respectively. Fe deficiency tolerance of these genotypes was related to the root release of PS which enabled a higher uptake of Fe in the IDTR than the IDSR under Fe deficiency. In general, IDTR performed better than the IDSR as evident from a significant increase in total iron, active iron, chlorophyll content and grain and straw yield. IDSR produced the highest grain and straw yield under slow iron release nano clay complex source. Grain Fe content of the IDTR and IDSR increased by 18.9 and 13.4%, respectively, under recommended dose of Fe. The results identified the most effective soil management strategies for the alleviating Fe deficiency chlorosis and improving Fe nutrition of both IDTR and IDSR genotypes.     

氮素运筹对两个晚稻品种产量及其主要构成因素的影响

本研究采用农民习惯施肥、实地氮素管理、实时氮素管理以及实时氮素管理基础上施用基肥,探讨了集约化农田不同氮素管理对水稻品种赣晚籼30（GWX）和博优141（BY）的产量及产量主要构成因素的影响。结果表明,与农民习惯施肥相比,不同氮素优化管理分别对水稻的产量、地上生物量、吸氮量、收获指数和氮收获指数以及穗数、每株实粒数没有显著影响。每个水稻品种的产量与生物量和吸氮量均呈极显著相关性（P0.01）。施用氮肥处理赣晚籼30平均产量为7.8 t/hm2,平均生物量为16.4 t/hm2,地上部平均吸氮量为N 175.6 kg/hm2,博优141的平均产量为8.9 t/hm2,生物量16.1 t/hm2,吸氮量N为 150.6 kg/hm2。氮肥施用后,博优141的产量、收获指数、氮收获指数、每株总粒数、实粒数显著高于赣晚籼30（P0.05）,而博优141的吸氮量、结实率和千粒重都显著低于赣晚籼30（P0.05）。与农民习惯施肥相比,实时氮素管理节肥率最高,实地氮素管理节肥率最小; 基肥施用具有稳产作用。总之,在当前集约化农田管理中,适量减少氮肥施用并不会影响水稻产量及其主要构成因素,但氮肥减施的可持续性有待进一步研究。