Effect of nitrogen rate on plant nitrogen loss in winter wheat varieties 1

Gaseous nitrogen (N) loss from winter wheat (Triticum aestivum L.) plants has been identified, but has not been simultaneously evaluated for several genotypes grown under different N fertility. Two field experiments were initiated in 1993 and 1994 at the Agronomy Research Station in Stillwater and Perkins to estimate plant N loss from several cultivars as a function of N applied and to characterize nitrogen use efficiency (NUE). A total of five cultivars were evaluated at preplant N rates ranging from 30 to 180 kg·ha^‐1. Nitrogen loss was estimated as the difference between total forage N accumulated at anthesis and the total (grain + straw) N at harvest. Forage, grain, straw yield, N uptake, and N loss increased with increasing N applied at both Stillwater and Perkins. Significant differences were observed among varieties for yield, N uptake, N loss, and components of NUE in forage, grain, straw, and grain + straw. Estimates of N loss over this two‐year period ranged from 4.0 to 27.9 kg·ha^‐1 (7.7 to 59.4% of total forage N at anthesis). Most N losses occurred between anthesis and 14 days post‐anthesis. Avoiding excess N application would reduce N loss and increase NUE in winter wheat varieties. Varieties with high harvest index (grain yield/total biomass) and low forage yield had low plant N loss. Estimates of plant loss suggest N balance studies should consider this variable before assuming that unaccounted N was lost to leaching and denitrification.     

Optimization of nitrogen fertilizer rate under integrated rice management in a hilly area of Southwest China

China has the world''s highest nitrogen (N) application rate, and the lowest N use efficiency (NUE). With the crop yield increasing, serious N pollution is also caused. An in-situ field experiment (2011-2015) was conducted to examine the effects of three N levels, 0 (i.e., no fertilizer N addition to soil), 120, and 180 kg N ha^-1, using integrated rice management (IRM). We investigated rice yield, aboveground N uptake, and soil surface N budget in a hilly region of Southwest China. Compared to traditional rice management (TRM), IRM integrated raised beds, plastic mulch, furrow irrigation, and triangular transplanting, which significantly improved rice grain yield, straw biomass, aboveground N uptake, and NUE. Integrated rice management significantly improved ¹⁵N recovery efficiency (by 10%) and significantly reduced the ratio of potential ¹⁵N loss (by 8%-12%). Among all treatments, the 120 kg N ha^-1 level under IRM achieved the highest ¹⁵N recovery efficiency (32%) and ¹⁵N residual efficiency (29%), with the lowest ¹⁵N loss ratio (39%). After rice harvest, the residual N fertilizer did not achieve a full replenishment of soil N consumption, as the replenishing effect was insufficient (ranging from -31 to -49 kg N ha^-1). Furthermore, soil surface N budget showed a surplus (69-146 kg N ha^-1) under all treatments, and the N surplus was lower under IRM than TRM. These results indicate IRM as a reliable and stable method for high rice yield and high NUE, while exerting a minor risk of N loss. In the hilly area of Southwest China, the optimized N fertilizer application rate under IRM was found to be 100-150 kg N ha^-1.     

不同的水稻品种产量及生理氮素利用效率的差异

Efficient use of N in agricultural practice can increase yield, decrease production costs and reduce the risk of environmental pollution. Effects of N fertilizer application rates on grain yield and physiological N use efficiency (PE) in relation to the accumulation and redistribution of biomass and N in rice (Oryza sativa L.) cultivars were studied at two experimental farms of Nanjing Agricultural University, Nanjing, China in 2004. Three high N use efficiency (NUE) rice cultivars (Wuyunjing 7, Nanguang and 4007) and one low NUE rice cultivar (Elio) with similar growth patterns were studied under seven N rates (0, 60, 120, 180, 240, 300 and 360 kg ha^-1). Grain yield increased with the N application rate and attained plateau at 180 kg N ha^-1 for rice cultivars at each site. Increasing N rate decreased PE for biomass and grain yield. Grain yield and PE of Elio were about 20% and 18% lower than those of high NUE cultivars. Differences in biomass, N accumulation and N redistribution were observed at the post-heading stage among rice cultivars with differing NUEs. The less reproductive tillers of Elio resulted in less demand for C and N during grain filling, thus leading to lower PE of Elio compared with the high NUE rice cultivars.     

Excessive nitrogen application decreases grain yield and increases nitrogen loss in a wheat–soil system

Abstract

Excessive use of nitrogen (N) fertilizers in wheat fields has led to elevated NO₃-N concentrations in groundwater and reduced N use efficiency. Three-year field and ¹⁵N tracing experiments were conducted to investigate the effects of N application rates on N uptake from basal and topdressing ¹⁵N, N use efficiency, and grain yield in winter wheat plants; and determine the dynamics of N derived from both basal and topdressing ¹⁵N in soil in high-yielding fields. The results showed that 69.5–84.5% of N accumulated in wheat plants derived from soil, while 6.0–12.5%and 9.2–18.1% derived from basal ¹⁵N and top ¹⁵N fertilizer, respectively. The basal N fertilizer recovery averaged 33.9% in plants, residual averaged 59.2% in 0–200 cm depth soil; the topdressing N fertilizer recovery averaged 50.5% in plants, residual averaged 48.2% in 0–200 cm soil. More top ¹⁵N was accumulated in plants and more remained in 0–100 cm soil rather than in 100–200 cm soil at maturity, compared with the basal ¹⁵N. However, during the period from pre-sowing to pre-wintering, the soil nitrate moved down to deeper layers, and most accumulated in the layers below 140 cm. With an increase of N fertilizer rate, the proportion of the N derived from soil in plants decreased, but that derived from basal and topdressing fertilizer increased; the proportion of basal and top ¹⁵N recovery in plants decreased, and that of residual in soil increased. A moderate application rate of 96–168 kg N ha^?1 led to increases in nitrate content in 0–60 cm soil layer, N uptake amount, grain yield and apparent recovery fraction of applied fertilizer N in wheat. Applying above 240 kg N ha^?1 promoted the downward movement of basal and top ¹⁵N and soil nitrate, but had no significant effect on N uptake amount; the excessive N application also obviously decreased the grain yield, N uptake efficiency, apparent recovery fraction of applied fertilizer N, physiological efficiency and internal N use efficiency. It is suggested that the appropriate application rate of nitrogen on a high-yielding wheat field was 96–168 kg N ha^?1.     

改善根系生长捕获深层土中NO3-N最优化免耕玉米氮肥利用率

Subsoil acidity restricts root growth and reduces crop yields in many parts of the world. More than half of the fertilizer nitrogen(N) applied in crop production is currently lost to the environment. This study aimed to investigate the effect of gypsum application on the efficiency of N fertilizer in no-till corn(Zea mays L.) production in southern Brazil. A field experiment examined the effects of surface-applied gypsum(0, 5, 10, and 15 Mg ha~(-1)) and top-dressed ammonium nitrate(NH_4NO_3)(60, 120, and 180 kg N ha~(-1)) on corn root length, N uptake, and grain yield. A greenhouse experiment was conducted using undisturbed soil columns collected from the field experiment site to evaluate NO_3-N leaching, N uptake, and root length with surface-applied gypsum(0 and 10 Mg ha~(-1)) and top-dressed NH_4NO_3(0 and 180 kg N ha~(-1)). Amelioration of subsoil acidity due to gypsum application increased corn root growth,N uptake, grain yield, and N use efficiency. Applying gypsum to the soil surface increased corn grain yield by 19%–38% and partial factor productivity of N(PFPN) by 27%–38%, depending on the N application rate. Results of the undisturbed soil column greenhouse experiment showed that improvement of N use efficiency by gypsum application was due to the higher N uptake from NO_3-N in the subsoil as a result of increased corn root length. Our results suggest that ameliorating subsoil acidity with gypsum in a no-till corn system could increase N use efficiency, improve grain yield, and reduce environmental risks due to NO_3-N leaching.     

稻草全量还田下氮肥运筹对双季晚稻产量及其氮素吸收利用的影响

【目的】稻草还田和合理的氮肥运筹不仅可以改良土壤和培肥地力,提高农作物产量和品质,还可以减少因过量施用氮肥带来的环境污染。随着水稻机械化收割的快速发展,稻草全量原位还田面积迅速扩大。因此,研究稻草全量还田后合理施用氮肥十分必要。本文通过早稻机收稻草切碎全量还田后晚稻氮肥运筹试验,探索该条件下晚稻氮肥的合理施用技术。【方法】以超级晚稻品种淦鑫688为试验材料,设计4个施氮（N）水平（0、 120、 180、 240 kg/hm2）基蘖穗肥比例为5∶2∶3,并在180 kg/hm2水平下增设稻草不还田对照处理和稻草全量还田下基蘖穗肥不同施氮比例处理（5∶0∶5、 5∶1∶4、 5∶2∶3、 5∶3∶2、 5∶4∶1、 5∶5∶0）。旨在分析不同处理间水稻产量、 产量构成和氮素吸收利用的差异。【结果】稻草全量还田下,施氮量在180 kg/hm2以下时产量随施氮量的增加而增加,之后则下降,处理间差异极显著。随施氮量的增加,有效穗数显著增加,而结实率则显著下降,施氮处理每穗粒数和千粒重显著高于不施氮处理。在相同施氮水平下,因为有效穗数、 结实率和千粒重显著提高,所以稻草全量还田产量极显著高于不还田处理,增幅8.83%。稻草全量还田同一施氮水平下,施氮比例为 5∶2∶3 处理产量极显著高于其他处理,其每穗粒数和千粒重均为最高,有效穗数随分蘖肥比例的增加而减少,处理间结实率差异不显著。稻草全量还田后,随着施氮量增加,其氮素总积累量、 氮肥表观利用率、 氮素的吸收率和百公斤籽粒的需氮量也显著提高,且与施氮量呈极显著正相关。但氮素收获指数和氮肥生理利用率均随施氮量的增加而降低。同一施氮水平下,全量还田处理水稻氮肥农学利用率和生理利用率均显著高于不还田处理。相关分析表明, 氮素总积累量与产量呈二次抛物线极显著正相关,氮肥表观利用率、 氮素吸收率与产量呈极显著正相关。稻草全量还田相同施氮水平下,随着穗肥施氮比例降低,其氮素总积累量、 中期的积累量和比率下降,其氮肥的表观利用率、 收获指数和氮素的吸收率也随之降低,但前期的氮素积累量和比率则升高。氮素的农学利用率和生理利用率均表现为随着穗肥比例的减少呈先增加后降低趋势,均以施氮比例为5∶2∶3处理处理最高,不施穗肥处理最低。各施氮比例处理中,穗肥的施氮量与氮素的总积累量、 中期积累量、 氮肥的表观利用率、 收获指数和氮素吸收率呈极显著正相关,氮肥的农学利用率和生理利用率与产量显著正相关。【结论】稻草全量还田后配施适量的氮肥可以提高晚稻产量,本试验以配施N 180 kg/hm2产量最高;在施纯N 240 kg/hm2以内,施氮越多,氮素积累量越多,相应的氮肥表观利用率、 氮素的吸收率和百公斤籽粒的需氮量也越大。总施氮量相同条件下,以基肥∶分蘖肥∶穗肥为 5∶2∶3 的施氮比例水稻产量, 氮肥农学、 生理利用率均为最高,此结果可作为双季稻区稻草全量还田后的推荐施氮比例。     

小麦根蘖发育和产量对耕作和追氮方式以及施氮量的响应

  【目的】  黄淮平原小麦生产中大量施用氮肥,探讨不同耕作和施肥方式对小麦根蘖发育的影响,以期实现减氮不减产并提高氮肥利用率的目标。  【方法】  2016—2018年连续两个种植年度,以半冬性中熟小麦品种矮抗58为材料,采用裂裂区设计试验方法,主区为施氮量 (240 、180 kg/hm2),副区为耕作方式 (旋耕、深耕),副副区为追肥方式 (撒施、隔行开沟追肥、隔二行开沟追肥),研究了小麦根系生长和生理活性、主茎和分蘖发育动态与成穗、籽粒产量和氮肥利用率。  【结果】  小麦不同生育时期单株次生根数、根系活力、单位面积茎蘖数、叶面积指数 (LAI) 均随施氮量降低而降低。与旋耕相比,深耕条件下小麦生育中、后期单株次生根数和单位面积茎蘖数增多、根系活力提高、LAI增大。生育后期,隔行开沟追肥的单株次生根数、根系活力、单位面积茎蘖数和LAI最高,撒施次之,隔二行开沟追肥最低。减量施氮较常规施氮籽粒产量降低了2.41%,氮肥偏生产力、氮肥吸收效率和氮肥内在利用率分别增加了29.67%、25.69%和2.29%。与旋耕相比,深耕条件下籽粒产量增加了5.60%,氮肥偏生产力和氮肥吸收效率分别提高了4.48%和8.47%。不同追肥方式中,隔行开沟追肥的籽粒产量最高,氮肥偏生产力和氮肥吸收效率显著提高,较撒施分别提高了3.62%、3.98%和7.38%,较隔二行开沟追肥分别提高了5.93%、6.34%和12.93%。  【结论】  深耕可提高生育中、后期小麦单株次生根数、根系活力和单位面积茎蘖数。常规施氮 (纯氮240 kg/hm2) 结合深耕 (深度25～30 cm)、隔行开沟追肥,可获得最高小麦产量;减施25%氮肥 (180 kg/hm2) 会导致籽粒产量降低,但结合深耕并采用隔行开沟施肥方式,可显著提高氮肥利用率,部分降低减氮所造成的产量损失,是获得高产高效的最佳组合。     

氮施用量对夏玉米土壤水氮动态及水肥利用效率的影响

采用田间小区试验,监测夏玉米不同生长期土壤水分和硝态氮剖面含量变化,研究不同施氮量对其时空变化及籽粒产量、水肥利用效率的影响,探讨氮肥对水肥资源高效利用的调节作用。结果表明：不同施氮处理,土壤剖面水分和硝态氮随土壤深度的变化趋势基本一致,即表层50 cm土壤水分和硝态氮含量较高且呈降低态,50-110 cm相对较低且波动较小,灌浆期二者均达到最低值;各生长期表层50 cm土壤含水量呈不施氮处理均高于施氮处理,50-110 cm土层则相反;施氮能提高土壤硝态氮含量,土壤硝态氮运移受土壤水分状况和含量的影响,含量越高,向下移动越深;施氮能显著提高水分利用效率及籽粒产量,增产效果明显（增产28.52%-37.86%）,二者均以施氮240 kg/hm^2处理最高;随施氮量的增加籽粒产量及籽粒吸氮量和水分利用效率增幅均表现为先升高后降低之趋势,当施氮量超过240 kg/hm^2后,籽粒产量和水分利用效率提高并不显著;不施氮与施氮处理氮素生产力、氮肥利用率之间均存在极显著差异。在本试验条件下,从控制土壤硝态氮积累及取得较高的产量和氮素利用率综合考虑,夏玉米的适宜施氮量范围应控制在120-240 kg/hm^2较好。     

On‐farm comparison of variable rates of nitrogen with uniform application to maize on canopy reflectance,soil nitrate,and grain yield

Recent development in canopy optical‐sensing technology provides the opportunity to apply fertilizer variably at the field scale according to spatial variation in plant growth. A field experiment was conducted in Ottawa, Canada, for two consecutive years to determine the effect of fertilizer nitrogen (N) input at variable‐ vs. uniform‐application strategies at the V6–V8 growth stage, on soil mineral N, canopy reflectance, and grain yield of maize (Zea mays L.). The variable N rates were calculated using an algorithm derived from readings of average normalized difference vegetation index (NDVI) of about 0.8 m × 4.6 m, and N fertilizer was then applied to individual patches of the same size of NDVI readings (0.8 m × 4.6 m) within a plot (2184 m²). Canopy reflectance, expressed as NDVI, was monitored with a hand‐held spectrometer, twice weekly before tasseling and once a week thereafter until physiological maturity. Soil mineral N (0–30 cm depth) was analyzed at the V6 and VT growth stages. Our data show that both variable and uniform‐application strategies for N side‐dressings based on canopy‐reflectance mapping data required less amount of N fertilizer (with an average rate of 80 kg N ha^–1 as side‐dressing in addition to 30 kg N ha^–1 applied at planting), and produced grain yields similar to and higher nitrogen‐use efficiency (NUE) than the preplant fully fertilized (180 kg N ha^–1) treatment. No difference was observed in either grain yield or NUE between the variable‐ and uniform‐application strategies. Compared to unfertilized or fully fertilized treatments, the enhancements in grain yield and NUE of the variable‐rate strategy originated from the later N input as side‐dressing rather than the variation in N rates. The variable‐rate strategy resulted in less spatial variations in soil mineral N at the VT growth stage and greater spatial variations in grain yield at harvest than the uniform‐rate strategy. Both variable‐ and uniform‐application strategies reduced spatial variations in soil mineral N at the VT stage and grain yield compared to the unfertilized treatment. The variable‐rate strategy resulted in more sampling points with high soil mineral N than the uniform‐rate strategy at the VT stage.     

Effects of Nitrogen Fertilization on Upland Rice based on Pot Experiments

Abstract

Limited information is available regarding the utilization and loss of fertilizer nitrogen (N) applied to intensively managed upland rice. Effects of N fertilization on upland rice were conducted as N0 (no N applied), N225 (225 kg N · ha^?1), N300 (300 kg N · ha^?1), and N375 (375 kg N · ha^?1) in pot experiments. ¹⁵N‐labeled techniques were used in basal and topdressing N fertilizations. Results showed with the increase of N quantity applied, tiller, panicle numbers per pot, and spikelet number per panicle increased significantly (P<0.05). Chlorophyll b content of N225 and N300 were significantly higher than N0 (P<0.05), and net photosynthetic rate (Pn) of N300 increased significantly compared with N0 and N225. Under basal fertilization, N use efficiency (NUE) of root, stem, leaf, and grain in N300 was the highest. The NUE and loss rate ranged from 23.3% to 30.3% and 62.4% to 73.8%, respectively, under basal fertilization. They varied from 16.5% to 27.5% and 70.7% to 80.4%, respectively, under topdressing fertilization. The highest NUE was observed in N300 under basal fertilization. As increased quantities of N were applied, Pn and biological characteristics improved, thus crop yield of upland rice increased. Grain yield of N300 and N375 were significantly higher than that of N0 and N225 (P<0.01); however, there was no significant difference between them. Therefore, N fertilization with medium applied quantity under basal fertilization will facilitate growing, photosynthesis, and grain yield increase of upland rice.     

氮肥用量及其分施比例对棉花氮利用和土壤氮平衡的影响

The Yellow River valley is one of the three largest cotton production areas in China.An experiment was performed in cotton fields of Anyang,China from 2013 to 2014 to investigate the effects of nitrogen(N) application rate and the ratio between basal and topdressing N fertilizer on N balance in a soil-plant system,N use efficiency,and cotton yield.Five N application rates as treatments were applied with the same split application ratio.Half of the N(50% basal fertilizer) was applied at pre-planting and the other half(50% topdressing fertilizer) at the initial flowering stage.These treatments were:zero N(N0,control),90 kg N ha~(-1)(N90(5/5)),180 kg N ha~(-1)(N180(5/5)),270 kg N ha~(-1)(N270(5/5),a reduced N rate),and 360 kg N ha~(-1)(N360(5/5),a conventional N rate).Additional 2 split application ratios as treatments were applied with the same N rate of 270 kg N ha~(-1).The split application ratios between basal N and topdressing N were 30%:70%(N270(3/7)) and 70%:30%(N270(7/3)).Results demonstrated that soil NH_4-N content in the 0–60 cm layer and NO3-N content in the 0–20 cm layer increased with increased N rate at the squaring and boll-opening stages and then decreased to lower levels at the initial flowering and harvest stages.Soil NO_3-N content in the 20–60 cm layer after the initial flowering stage increased with the increase of topdressing N rate.Soil apparent N surplus varied at different growth stages,while the soil apparent N surplus over the entire growth period exhibited a positive relationship at N rates over 180 kg ha~(-1).Seed cotton yield of N270(3/7) was the highest of all treatments.Plant N uptake,N agronomic efficiency,and apparent N recovery efficiency of N270(3/7) were significantly higher than those of N270(5/5) and N270(7/3) in both growing seasons.These suggest both economic and ecological benefits in cotton production in the Yellow River valley could be created,by appropriately reducing total N application rate and increasing the ratio of topdressing to basal N fertilizer at the initial flowering stage.     

限水灌溉下施氮量对冬小麦产量、氮素利用及氮平衡的影响

2008~2009年通过大田试验,研究了限水灌溉条件下,不同施氮量对冬小麦产量、氮素利用、土壤硝态氮动态变化及氮素平衡的影响。结果表明,施用氮肥显著增加小麦穗数和穗粒数,对千粒重无显著影响。作物产量、吸氮量与施氮量均呈抛物线关系,施氮量超过N240 kg/hm2,产量和吸氮量随施氮量增加略有降低。小麦起身期后,0—100 cm土层都有硝态氮分布,且随土层深度增加而减少;相同土层则随施氮量的增加而增加。土壤硝态氮积累量随生育期推进而降低,N0和N120处理分别在拔节期和开花期后表现出氮素亏缺;成熟期,土壤表观盈余以残留为主,表观损失量占小部分。氮肥表观利用率、农学利用率随施氮量增加呈降低趋势,而氮素残留率随施氮量增加呈增加趋势。在本试验条件下,施氮量在N 180~220 kg/hm2水平可以达到产量、氮素表观利用率、氮素残留率的较好结合,是限水灌溉下兼顾经济效益与环境效益的适宜施氮量。     

中国玉米小麦产量与氮肥利用效率同步提高的研究进展

Achieving both high yield and high nitrogen use efficiency (NUE) simultaneously has become a major challenge with increased global demand for food, depletion of natural resources, and deterioration of environment. As the greatest consumers of N fertilizer in the world, Chinese farmers have overused N and there has been poor synchrony between crop N demand and N supply because of limited understanding of the N uptake-yield relationship. To address this problem, this study evaluated the total and dynamic N requirement for different yield ranges of two major crops (maize and wheat), and suggested improvements to N management strategies. Whole-plant N aboveground uptake requirement per grain yield (N _req) initially deceased with grain yield improvement and then stagnated, and yet most farmers still believed that more fertilizer and higher grain yield were synonymous. When maize yield increased from < 7.5 to > 12.0 Mg ha^-1, Nreq decreased from 19.8 to 17.0 kg Mg^-1 grain. For wheat, it decreased from 27.1 kg Mg^-1 grain for grain yield < 4.5 Mg ha^-1 to 22.7 kg Mg^-1 grain for yield > 9.0 Mg ha^-1. Meanwhile, the percentage of dry matter and N accumulation in the middle-late growing season increased significantly with grain yield, which indicated that N fertilization should be concentrated in the middle-late stage to match crop demand while farmers often applied the majority of N fertilizer either before sowing or during early growth stages. We accordingly developed an integrated soil-crop system management strategy that simultaneously increases both grain yield and NUE.     

Field-level comparison of nitrogen rates and application methods on maize yield,grain quality and nitrogen use efficiency in a humid environment

Appropriate nitrogen (N) management practices are of critical importance in improving N use efficiency (NUE), maize (Zea mays) yield and environmental quality. A six-year (2005–2010) on-farm trial was conducted in Ottawa, Canada to assess the effects of N rates and application methods on grain yield and NUE. In four out of the six-year study, grain yield increased by 60–77 kg ha^?1 by sidedress, compared to 49–66 kg ha^?1 for each kg N ha^?1 applied at preplant. Grain yield response to N between the two strategies was similar in the other growing seasons. Sidedress strategy required 15 kg N ha^?1 less of the maximum economic rate of N (MERN) than preplant application. Our results indicate that sidedress application of 90–120 kg N ha^?1 with a starter of 30 kg N ha^?1 resulted in greater yield, grain quality and NUE than preplant N application in this cool, humid and short growing-season region.     

Effect of time of application on nitrogen uptake,partitioning, and remobilization in walnut trees

Walnut tree requires a relatively high amount of nitrogen (N). To avoid loss in the environment, N uptake efficiency (NUE) should be optimized. The aims of this study were to evaluate the effect of time of N application on NUE, partitioning, and remobilization in walnut trees. Two-year-old trees were planted in 40-L pots and fertilized with 1 g of ¹⁵N-enriched (5 atom %) N at: 1) bud burst, 2) pistillate flower maturity, and 3) late summer. One week after fertilization, the percentage of N derived from fertilizer and NUE were higher in trees fertilized in late summer, than other timings. N uptake was linearly related to root dry weight. At May 2008 harvest, the N stored in trunk and twigs was remobilized to the developing leaves and to the roots. Late summer N application appeared to be the most effective in providing N for walnut spring new growth.     

前氮后移对水稻产量形成和田面水氮素动态变化的影响

通过田间小区试验,在施氮量180 kg/hm~2水平下,设置4个氮肥运筹比例,基肥∶分蘖肥∶穗肥的比例分别为10∶0∶0(T1),4∶3∶3(T2),2∶3∶5(T3),0∶3∶7(T4),研究氮肥后移对水稻产量形成和稻田田面水氮素动态变化的影响。结果表明:与氮肥全部作为基肥施用的处理相比,将前期氮肥的30%甚至50%后移到穗肥施用,对水稻产量没有明显影响,而氮肥后移70%至穗肥会使水稻产量显著下降。田面水中总氮(TN)和可溶性总氮(DTN)浓度在每次施肥后1天达到峰值,铵态氮(NH_4~+-N)浓度在基肥和分蘖肥后1天达到峰值,穗肥后3天达到峰值,随后逐渐降低至与不施氮肥处理相当。整个基肥期、分蘖肥后20天内和穗肥后9天内是防止稻田氮素流失的关键期。施尿素后,DTN是田面水氮素的主要部分,DTN以无机氮(IN)为主,而NH_4~+-N在IN中所占比例达64.0%以上。比较水稻生育过程中氮素流失风险期内的TN、DTN和NH_4~+-N三氮浓度,相比T1,T2的三氮浓度分别降低了2.9%,1.6%,3.1%,T3的三氮浓度分别降低了15.5%,14.7%,22.3%,T4的三氮浓度分别降低了16.1%,22.9%,34.1%,结合产量,确定基肥∶分蘖肥∶穗肥比例为2∶3∶5的氮肥后移措施能够在保证水稻产量不下降的同时,有效降低稻田氮素的流失风险。     

Different rates of biochar application change 15N retention in soil and 15N utilization by maize

Biochar application to soil may impact soil nitrogen (N) dynamics, but the effects on N uptake and utilization by crop remain largely unknown, especially the effects of the rate of biochar application. To investigate the effects of biochar on soil ¹⁵N retention rate and ¹⁵N utilization efficiency (¹⁵NUE) by maize, a six-month ¹⁵N isotope tracer technique combined with in situ pot experiment was conducted in Mollisol. The experiment included four treatments: no biochar applied (CK) and biochar applied at the rates of 12 t ha⁻¹ (P12), 24 t ha⁻¹ (P24) and 48 t ha⁻¹ soil (P48). Compared with CK, biochar application reduced soil bulk density and ¹⁵N loss rate, and significantly improved total N and ¹⁵N retention amount in the 0–30 cm soil depth. The P24 treatment had the largest increase in ¹⁵N retention rate throughout the 0–40 cm depth. After biochar application, the ¹⁵N uptake and ¹⁵NUE were significantly increased in the grain and leaf, which promoted grain yields. Contrary to this, the P48 treatment appeared to lower ¹⁵N uptake and ¹⁵NUE compared with P12 and P24. In conclusion, biochar application improves the potential of the soil to retain N and the improvement in ¹⁵N uptake and utilization are more pronounced in maize leaves and grain. Moreover, biochar application promotes ¹⁵N utilization in maize plant and improves maize yield. However, when biochar application rate is high (i.e. P48 treatment), the ¹⁵N retention by the soil and ¹⁵N utilization by the maize are reduced markedly compared with P12 and P24.     

秸秆覆盖与氮肥运筹对杂交稻根系生长及氮素利用的影响

【目的】在我国稻-麦、稻-油等多熟制区域,富含氮素的小麦、油菜等水稻前茬作物秸秆被大量弃置、焚烧,造成极大浪费和环境污染,与此同时,稻季氮肥投入量却在逐年增加,因此在水稻生产中研究秸秆覆盖与氮肥配合施用的理论与技术,对实现秸秆还田与减少氮肥用量具有重要意义。本试验研究油菜、小麦2种秸秆覆盖方式下,3种不同的氮肥运筹方式对杂交稻主要生育时期根系生长、氮素吸收利用特征及产量的影响,并探讨其根系生长与氮素利用及产量间的关系,以期寻求最佳的秸秆还田与氮肥运筹搭配模式。【方法】本试验以杂交稻F优498为材料,采用两因素裂区试验设计,主区为小麦秸秆覆盖(S1)、油菜秸秆覆盖(S2)和无秸秆覆盖(S0);副区为氮肥运筹模式,在135 kg/hm2总施氮量条件下,设置基肥∶蘖肥∶穗肥为5∶3∶2(N1);基肥∶蘖肥∶穗肥为3∶3∶4(N2);基肥∶蘖肥∶穗肥为3∶1∶6(N3)3种氮肥运筹模式,以不施氮肥(N0)为对照。研究各处理杂交稻在移栽后20 d、移栽后30 d、齐穗期和成熟期根系生长及形态、各生育期的干物质与氮素积累,水稻茎鞘的干物质转运、产量及其构成因子以及各时期氮素积累及利用效率,同时对各生育时期根系生长与氮素利用及产量间的关系进行分析。【结果】结果表明,小麦秸秆覆盖均可有效促进杂交稻各生育时期的根系生长、改善根系形态、增加各时期的干物质与氮素积累,提高氮肥的利用效率及稻米产量。在不同种类秸秆覆盖下,基肥∶蘖肥∶穗肥(倒4叶龄期施入)为3∶3∶4(N2)时,可及时地对杂交水稻主要生育时期的根系生长进行调控,有效促进抽穗至成熟期的干物质积累与转运率,提高水稻主要生育时期的氮素积累及氮肥利用效率,显著增加稻谷产量,为本试验中最优的氮肥管理模式;而氮肥后移比例过高(基肥∶分蘖肥∶穗肥运筹比例为3∶1∶6),会限制齐穗期根系的生长,导致稻谷产量及氮肥利用效率降低。相关性分析表明,秸秆覆盖与氮肥运筹下主要生育时期根干重、根体积、总根长与产量及氮素吸收利用均存在显著或极显著的正相关(r=0.38*0.78**),尤其以齐穗期的根体积与总根长、根干重与氮素累积、产量及氮素回收利用率的相关性最好。【结论】小麦秸秆、油菜秸秆覆盖能够有效促进杂交稻根系的生长,增加干物质与氮素积累,提高氮肥利用效率,且小麦秸秆覆盖效果更显著。秸秆覆盖条件下,氮肥运筹以基肥∶蘖肥∶穗肥为3∶3∶4时的水稻根系生长旺盛,物质生产能力强,氮肥利用效率最高。因此,小麦秸秆覆盖与基肥∶蘖肥∶穗肥以3∶3∶4的比例配合的水稻的产量最高,为最优组合。     

Timing and level of nitrogen supply affect nitrogen distribution and recovery in two contrasting oat genotypes

Human diets containing oat (Avena sativa L.) grain offer health benefits resulting in an emerging interest in oat improvement. Information on nitrogen (N) uptake, distribution, and use efficiency (NUE) in oat is limited. A greenhouse study using a ¹⁵N‐labeling technique was conducted to determine the responses of two contrasting oat genotypes to timing and level of N deficiency. Hulled oat cv. Prescott and hulless cv. AC Gehl were grown in soil‐mix pot culture with five N treatments applied through modified Hoagland solutions. Differences in ¹⁵N accumulation, ¹⁵N distribution, plant N originating from the labeled source, and NUE between the contrasting cultivars, were examined for each N strategy. Level of N deficiency and timing of N supply of ¹⁵NH₄¹⁵NO₃ greatly affected ¹⁵N distribution, the origins of plant N, and the amount of ¹⁵N recovered in the plant. When N was supplied from seedling emergence to maturity (T₁), AC Gehl accumulated 61% more ¹⁵N in the shoots, but 46% less ¹⁵N in the grain than Prescott (0.43 vs. 0.80 mg plant^–1), indicating that AC Gehl was less effective in producing grain yield than Prescott as AC Gehl produced greater total dry matter (DM). Withholding N supply until flag‐leaf stage (FL) increased ¹⁵N in the grain of both cultivars by 29.6%, resulting in the highest NUE. In most cases, there were larger portions of plant N derived from the labeled source for AC Gehl than for Prescott. Our results suggest that greater NUE in the newly released AC Gehl was associated with N accumulation in the vegetative tissues. It is concluded that genotype improvement of hulless oat should be focused on enhancing N‐translocation efficiency.     

氮、硫配施对冬小麦氮素利用效率及产量的影响

【目的】氮(N)、硫(S)是生物所必需的营养物质,对小麦籽粒产量和品质起着重要作用。硫素供应不足,特别是在当前大量氮素供应情况下引起的作物生理性缺硫将导致作物产量和含硫氨基酸蛋白质含量下降。本文旨在探索氮、硫配施对冬小麦氮素利用效率和籽粒产量的促进效果并提出合理的区域氮、硫施肥技术。【方法】20122013年,在河南温县以国审冬小麦品种豫麦49-198为供试材料,进行大田试验。设置不同施氮量0、120、180、240和360 kg/hm2(分别以N0、N120、N180、N240和N360表示)和施硫0和60 kg/hm2(S0和S60)试验,调查氮、硫对冬小麦干物质积累、氮素积累分配、籽粒产量和氮素利用效率的影响。【结果】对冬小麦生育后期干物质积累分析表明,干物质积累随施氮量增多而提高,相同施氮量条件下施硫较不施硫小麦干物质积累量显著提高,其中成熟期干物质积累量N180S60、N240S60和N360S60分别较N180S0、N240S0和N360S0提高2225、3607和3120 kg/hm2,而且氮素低的处理添加硫后干物质积累量高于氮素高不加硫处理,如N180S60N240S0、N240S60N360S0,处理间差异均达显著水平。随施氮量增多,冬小麦植株氮素积累总量增加,在N 240和360 kg/hm2水平,硫素供应显著增加小麦植株氮素积累。不同施氮量条件下施硫较不施硫均显著提高了小麦籽粒产量,分别提高了10.5%、18.3%、5.2%、5.6%和4.9%。随施氮量增多,氮肥偏生产力下降,氮回收效率、生理效率和农学效率则均以N 180达最高值。不同施氮水平下,施硫均显著提高了冬小麦氮素回收效率,但对氮生理效率影响不显著,其中在施N量为120、180和240kg/hm2时,施硫较不施硫氮肥偏生产力和农学效率均显著提高。【结论】在当前小麦生产中,采用控氮或减氮增硫技术措施,可实现小麦氮利用效率和籽粒产量的同步提高。在本试验地区小麦生产中,达到冬小麦稳产高效或增产高效的适宜施氮量为180 240 kg/hm2配合60 kg/hm2硫肥施用。