植物提取物皂甙元对仔猪断奶应激的影响

采用单因子试验设计。选择 96头 2 8日龄大×长×北三元杂交断奶仔猪 ,随机分成 3组 ,研究饲料中添加不同剂量 (0、5 0、10 0mg/kg)植物性抗应激复合添加剂 (即植物提取物 ,以酸枣仁皂甙元为主 )对仔猪生产性能的影响。试验期 2 8d。试验结果表明 :饲料中添加植物提取物 5 0mg/kg和 10 0mg/kg ,日增重提高 7.1%～ 9.2 %(P <0 .0 5 )、采食量增加 3.5 %～ 4 .9%(P <0 .0 5 ) ,饲料转化率改善 (F/G) 3.2 %～ 3.7%(P <0 .0 5 ) ,仔猪腹泻率明显降低 ,皮毛光泽 ,行为安祥。     

氟苯尼考静注及肌注在鸡体内药代动力学研究

健康AA肉鸡 36只 ,随机分成 4组 ,以 15mg/kg和 30mg/kg两种剂量静注、肌注分别给予氟苯尼考。用高压液相色谱法测定血浆中的药物浓度 ,采用 3p97药代动力学程序软件处理药 时数据。静注药 时数据符合二室开放模型 ,主要药代动力学参数 :15mg/kg剂量组Vd(ss) 1 5 7± 0 16L/kg ,T1/2α43 96± 12 2 7min、T1/2 β16 8 18±45 2 4min、CL(s) 0 0 17± 0 0 0 30L/ (kg·min)、AUC886 40± 146 5 3(μg/ml)·min ;30mg/kg剂量组Vd(ss) 1 42±0 2 3L/kg ,T1/2α41 48± 8 6 4min、T1/2 β180 80± 74 97min、CL (s) 0 0 17± 0 0 0 2 9L/ (kg·min)、AUC176 7 15±2 6 8 2 3(μg/ml)·min。肌注药 时数据符合一室开放模型 ,主要药代动力学参数 :15mg/kg剂量组T1/2 (ka) 10 2 5±9 19min、T1/2 (ke) 15 2 41± 73 0 9min、C(max) 3 5 0± 1 13μg/ml、AUC837 88± 16 0 85 (μg/ml)·min、F94 5 3% ;30mg/kg剂量组T1/2 (ka) 11 97± 7 5 9min、T1/2 (ke) 15 2 41± 73 0 9min、C(max) 6 79± 1 38μg/ml、AUC172 5 2 9±35 7 98(μg/ml)·min、F97 6 3%。实验结果表明 :氟苯尼考在鸡体内吸收好 ,分布快 ,消除也快。静注、肌注后曲线下面积AUC与剂量呈比例关系 ,各参数无剂量依赖性。     

上市肉鸡组织样品中铅、镉、铜、铬含量的测定

从江苏省某市采集上市肉鸡组织样品 ,采用微波消解 石墨炉 原子吸收分光光度法 ,测定主要组织 (心脏、肝脏、肌胃、胸肌、肾脏、腿肌、骨骼 )中铅、镉、铜、铬等重金属元素的含量。检验结果 :组织中Pb的含量以心脏最高 (1 3478± 0 531 2 )mg/kg ,肝脏次之 (1 1 2 6 3±0 534 0 )mg/kg ,骨骼最低 (0 1 0 1 8± 0 0 0 6 6)mg/kg ,样品含Pb超标率按上述组织顺序分别为 :1 0 0 %、 96 87%、 96 87%、 87 50 %、1 0 0 %、 81 2 5 %和 0 ;组织中Cd的含量以肾脏最高 (0 0 63 0± 0 0 2 0 2 )mg/kg,骨骼次之 (0 0 55 9± 0 0 2 0 5)mg/kg ,样品含Cd超标率分别为 :6 2 5 %、 0、 3 1 3 %、 0、 0、 0和 0 ;组织中Cu的含量以心脏最高 (8 492 7± 1 90 1 2 )mg/kg,肝脏次之 (8 462 8± 1 666 4)mg/kg ,腿肌最低 (0 8373± 0 741 4 )mg/kg ,样品含Cu超标率分别为 :1 2 50 %、 1 5 63 %、 9 38%、 3 1 3 %、 0、 0和 0 ;组织中Cr的含量以肾脏最高(1 642 5± 1 0 1 0 0 )mg/kg ,肝脏次之 (1 642 5± 0 61 97)mg/kg ,样品含Cr超标率分别为 :50 0 0 %、 62 70 %、 9 38%、 3 1 3 %、 0、 0和 0。检测数据表明 ,鸡主要组织样品中Pb含量严重超标 ;肝脏、肾脏中Cr超标较严重 ,Cd和Cu也有不同程度?     

日粮中添加大豆黄酮对鹌鹑生产性能和血液中激素水平的影响

本试验采用了 3个生产阶段的蛋用鹌鹑。 35日龄开产期蛋用鹌鹑日粮中添加 3mg/kg大豆黄酮 ,鹌鹑的产蛋率和血液中T3 水平均发生显著性提高 (P <0 0 5 )。 7月龄蛋用鹌鹑日粮中添加 3mg/kg大豆黄酮 ,可显著提高鹌鹑的产蛋率 (P <0 0 5 )和血液中T3 水平 (P <0 0 5 ) ;而日粮中添加 6mg/kg大豆黄酮则使鹌鹑的产蛋率显著下降 (P <0 0 5 )。 12月龄蛋用鹌鹑日粮中添加 6mg/kg大豆黄酮 ,鹌鹑的产蛋率提高 10 3% (P <0 0 1) ;血液中T3 、T4和IGF 1水平分别提高 34 8% (P <0 0 1)、2 7 6 % (P <0 0 5 )和 30 8% (P <0 0 5 )。本次研究结果表明 ,日粮中添加一定剂量大豆黄酮能显著增加开产期、高峰期和后期蛋用鹌鹑的产蛋率 ,并影响其内分泌机能     

牛磺酸对蛋鸡抗氧化能力的影响

选用 1 0 8只体况良好、采食量、产蛋量无显著差异的 78周龄蛋用母鸡 ,随机分为 4组 ,1个对照组 ,3个试验组 ,每组 3个重复 ,每个重复 9只鸡。日粮中分别添加 0mg/kg ,50mg/kg ,1 0 0mg/kg ,2 0 0mg/kg牛磺酸 ,饲喂 56d。结果表明各试验组血清SOD活性与对照组相比 ,分别提高 5 68%、 7 99%和 7 2 8% ;血清MDA含量与对照组相比 ,分别降低 6 32 %、 1 0 1 1 %和 1 0 2 9% ;蛋黄MDA含量与对照组相比 ,分别降低3 1 8%、 6 30 %和 6 0 3 %。     

酪蛋白铜对仔猪生长及血清铜蓝蛋白和SOD活性的影响

选取体重 2 0kg左右杜长大仔猪 90头 ,随机分为 3组 ,分别饲喂添加 5 ,2 4 0mg/kg铜 (CuSO4·5H2 O)和5 0mg/kg酪蛋白铜的饲粮 ,试验期 3 0d ,试验第 1 5d和结束时对猪只称重 ,探讨酪蛋白铜对仔猪生长性能的影响 ,并对血液相关指标进行分析。结果表明 ,与 5mg/kg硫酸铜组相比 ,在生长前期 ,2 4 0mg/kg硫酸铜 (高铜 )的促生长效果最佳 ,日增重提高了 1 2 .3 %(P <0 .0 5 ) ,酪蛋白铜组日增重提高了 9.5 %(P <0 .0 5 ) ,但在生长后期 ,酪蛋白铜促生长作用超过了高铜 ,日增重提高了 1 7.0 %(P <0 .0 5 )。就整个生长期而言 ,酪蛋白铜的促生长效果最佳 ,日增重提高了 1 3 .6 %(P <0 .0 5 )。高铜明显降低了饲料转化率 ,降幅为 9.5 %(P <0 .0 5 ) ;高铜和酪蛋白铜组铜蓝蛋白活性分别提高了 3 4 .2 8%和 3 5 .71 %(P <0 .0 1 ) ,SOD分别提高 76 .4 %和 80 .8%(P <0 .0 1 )。由此可见 5 0mg/kg酪蛋白铜可显著影响猪的生长及血清相关指标水平 ,达到高剂量硫酸铜的效果     

国产脱脂鱼粉的存储稳定性研究

本试验旨在研究国产脱脂鱼粉在夏季常规仓储（18～27℃，50%～70%RH）、模拟冬季存储（12℃，55%RH）和模拟夏季极端存储（35℃，85%RH）条件下的存储稳定性。结果表明：通风、低温和低湿环境有助于提高脱脂鱼粉的存储稳定性。夏季常规仓储和模拟冬季存储条件下脱脂鱼粉存放126 d后的微生物指标（霉菌总数<100CFU/g，未检出沙门氏菌）、水分含量（<10%）、水分活度（0.5～0.7）、酸价（<3.0 mg/g）、丙二醛含量（6～13 mg/kg）、挥发性盐基氮含量（<25 mg/100 g）和组胺含量（<50 mg/kg）均符合GB/T 19164—2021《饲料原料鱼粉》的限值要求。夏季极端存储条件下，脱脂鱼粉存放21 d后的霉菌总数就超过10000 CFU/g，变性变质极其严重。因此，脱脂鱼粉的冬季存储较为安全，而夏季存储需要严防高温高湿环境。     

磷酸泰乐菌素对早期断奶仔猪腹泻病预防效果的观察

将 2 1～ 2 8日龄的健康仔猪 1 6窝随机分成 4组 ,每组 4窝。Ⅰ组在日粮中添加磷酸泰乐菌素 33mg/kg;Ⅱ组在日粮中添加维生素C 5mg/kg和维生素E 5mg/kg ;Ⅲ组在日粮中添加 2 5mg/kg的磷酸泰乐菌素和维生素C 3mg/kg、维生素E 3mg/kg ;Ⅳ组为对照组 ,日粮中不添加任何药物。结果表明 :各试验组仔猪腹泻的发病率分别为 1 0 0 6 % ,1 6 67% ,3 33 % ,43 75 % ,试验组猪的发病率明显低于对照组 (P <0 0 1 )     

甜菜碱提高蛋鸡产蛋性能的机理研究

15 36只 30 0日龄罗曼蛋鸡 ,随机分为 4组 ,分别饲喂添加 0 ,5 0 0 ,10 0 0 ,15 0 0mg/kg甜菜碱的玉米—豆粕型日粮 ,研究甜菜碱提高蛋鸡产蛋性能的作用机制。结果表明 ,日粮中添加 10 0 0mg/kg甜菜碱对提高蛋鸡产蛋性能效果最佳 ,显著提高产蛋率 10 .11% (P <0 .0 5 ) ,提高饲料转化率 6 .4 1% (P <0 .0 5 )。进一步研究发现 ,日粮中添加 10 0 0mg/kg甜菜碱使血清促卵泡生成素 (FSH)、促黄体生成素 (LH)、极低密度脂蛋白胆固醇含量和脂肪酶活性分别升高了 5 6 .4 9%(P <0 .0 1)、86 .0 3% (P <0 .0 5 )、4 3.0 7% (P <0 .0 5 )和 30 .0 7% (P <0 .0 5 ) ,血清甘油三酯和尿酸含量分别下降了 2 2 .18%(P <0 .0 5 )、18.6 6 % (P <0 .0 5 )。上述结果提示 :甜菜碱通过增强腺垂体FSH和LH的分泌 ,同时促进卵蛋白和卵脂肪沉积 ,从而提高蛋鸡产蛋率和饲料利用率     

恩诺沙星混悬液在猪体内的药动学及生物利用度

本文比较了恩诺沙星混悬液和恩诺沙星溶液在猪体内的药动学特征和生物利用度。选用 7头健康猪按拉丁方设计进行静注、肌注恩诺沙星溶液和肌注恩诺沙星混悬液在猪体内的药物动力学研究。 3种给药方法的剂量均为 10mg/kg。猪静注给药的药时数据符合二室开放模型 ,主要药动学参数为 :t1/ 2α0 6 4± 0 15h ,t1/ 2 β9 0 6± 2 47h ,Vd(area) 4 40± 0 88L/kg ,ClB0 35± 0 0 6L·kg-1·h-1,AUC2 9 85± 4 11L·kg-1·h。猪肌注恩诺沙星溶液和恩诺沙星混悬液的药时数据符合一级吸收一室模型 ,其主要药动学参数分别为t1/ 2ka0 2 4± 0 10h和 1 2 5± 1 0 9h(P <0 0 5 ) ;t1/ 2ke8 90± 2 0 2h和 18 95± 4 5 5h(P <0 0 1) ;Tmax1 2 5± 0 41h和 5 14± 2 95h(P <0 0 1) ;Cmax1 5 4± 0 2 5 μg/ml和 0 87± 0 2 1μg/ml;AUC2 1 49± 4 94mg·L-1·h和 2 8 97± 10 80mg·L-1·h ;F72 0 %±17 4%和 97 7%± 35 0 %。比较肌注恩诺沙星混悬液和恩诺沙星溶液的主要药动学参数 ,二者有显著差异 ,前者的t1/ 2ka、Tmax、t1/ 2ke和Cmax分别为后者的 5 2、4 1、2 1和 0 6倍。这些差异说明恩诺沙星混悬液肌注后吸收缓慢 ,消除半衰期延长 ,临床应用 48h给药 1次仍能维持对常见病原菌的有效血药     

柞蚕幼虫、嫩蛹及滞育蛹营养成分的比较研究

为进一步开发柞蚕的可利用价值,对柞蚕幼虫、嫩蛹（神仙蛹）及滞育蛹的营养成分进行了分析比较,结果表明：蛋白质和脂肪含量均以滞育蛹最高,分别为59．2g／100g和12．1g／100g;总糖和粗多糖含量则以嫩蛹最高,分别为3．0g／100g和5．4g／100g,其中,嫩蛹的粗多糖比柞蚕幼虫高58．8％,比滞育蛹高74．2％;氨基酸总量嫩蛹最高为44．94g／100g,而且其天门冬氨酸和谷氨酸含量也高于柞蚕幼虫和滞育蛹,分别占氨基酸总量的10．68％和12．84％。柞蚕嫩蛹是营养价值较高的昆虫食品,具有较高的开发利用价值。     

CQ10-LPSp对紫花苜蓿幼苗内生细菌多样性及其根瘤菌的影响

为了探究成团泛菌CQ10脂多糖（Pantoea agglomerans CQ10 Lipopolysaccharide,CQ10-LPSp）对紫花苜蓿（Medicago sativa L.）幼苗内生细菌多样性及其根瘤菌（Rhizobium）的影响,本试验首先研磨分离‘巨能551’紫花苜蓿幼苗内生细菌,后设置6个不同浓度梯度（0,0.133 5,0.200 3,0.267 0,0.333 8,0.400 5 EU·mL^-1）的CQ10-LPSp与紫花苜蓿互作,再分离并测定了其根系内生细菌的种类和数量以及根瘤菌的数量。结果显示,随着CQ10-LPSp浓度的增大,紫花苜蓿内生根瘤菌的数量呈先升高后降低的变化趋势,在0.267 0 EU·mL^-1 CQ10-LPSp下根瘤菌数量最多,为6.98×10⁷ cfu·g^-1,且该浓度下根系内生细菌种类较种带细菌多样性减少。结果表明,CQ10-LPSp处理下会影响植物内生细菌的种类和数量,且在一定浓度范围内能增加紫花苜蓿内生根瘤菌的数量。本研究结果为开发促进紫花苜蓿内生根瘤菌生长的外源添加剂奠定一定的理论和实践基础。     

Evaluating the impact of excess dietary tryptophan on laying performance and immune function of laying hens reared under hot and humid summer conditions

1. Tryptophan (Trp), besides its role as an essential amino acid in protein synthesis, may also have other important effects on laying hens under summer conditions.

2. Babcock Brown layers (n?=?768), 40 weeks of age, were allocated to 4 treatment groups, each of which included 6 replicates of 32 hens. Each group received the same basal diet, formulated with maize and soybean meal, for 8 weeks. Hens were fed on the basal diet with 0·0, 0·2, 0·4, and 0·8?g/kg L-Trp to achieve dietary concentrations of 1·7, 1·9?g/kg, 2·1?g/kg or 2·5?g/kg of Trp, respectively.

3. Supplementing L-Trp had no affect on laying performance. Adding 0·2 or 0·4?g/kg L-Trp improved egg shell strength compared with those fed on the control diet. Serum albumin concentration increased at 0·4?g/kg compared with those receiving 0·0 or 0·8?g/kg Trp. The addition of Trp at 0·4?g/kg increased serum IgM concentration quadratically. Serum superoxide dismutase (T-SOD) increased linearly and quadratically at 0·4?g/kg.

4. In conclusion, we suggest that 0·2 to 0·4?g/kg Trp may have beneficial effects on laying hens under conditions of high temperature and humidity.     

柞蚕蛹溶菌酶的诱导与提取

本文报道热空气、大肠杆菌K_(12)D_(31)诱导柞蚕蛹(Antheraea pernyi)血淋巴溶菌酶活力提高的最适条件以及从柞蚕蛹免疫血淋巴中提取溶菌酶.以Sephadex G-50和CM-Sepharose柱层析法从柞蚕蛹免疫血淋巴中提取溶菌酶,并首次获得柞蚕蛹溶菌酶结晶,溶菌酶制剂比活力达到57353单位/mg蛋白,提高了257倍,总活力回收79.2%.首次应用选择性热变性,等电点沉淀、DEAE-纤维素离子交换层析、包被甲壳素-纤维素亲和层析等分离技术从柞蚕蛹免疫血淋巴中提取溶菌酶.溶菌酸制剂比活力达25 375单位/mg蛋白,提高了140倍,活力回收51%.用15%、pH4.0聚丙烯酰胺凝胶电泳(PAGE)和3.0-100%.pH6.7-8.9SDS-PAGE鉴定了酶制剂的纯度,并测定了柞蚕蛹溶菌酶的部分氨基酸组成.     

Effects of biotin on growth performance and foot pad dermatitis of starter White Pekin ducklings

1. An experiment with 9 dietary supplemental biotin concentrations (0, 0·03, 0·06, 0·09, 0·12, 0·15, 0·18, 0·21, 1·5?mg biotin/kg) was conducted to study the effects of supplementary dietary biotin on growth performance and foot pad dermatitis (FPD) of White Pekin ducklings from hatch to 21?d of age.

2. One-d-old male Pekin ducklings (n?=?576) were randomly divided into 9 dietary treatments, each containing 8 replicate pens with 8 birds per pen. Final weight, feed intake and body weight gain increased with increasing dietary biotin levels from hatch to 21?d of age. No differences were observed in feed conversion ratio.

3. The supplemental biotin requirement of ducklings for optimal body weight gain was estimated to be 0.180?mg/kg.

4. At 28?d of age, dehydration, cracks, bleeding and scab, and ulceration were observed in biotin-deficient ducks. The external scores for FPD decreased from 17·50 to 1·00 with increasing dietary biotin. It was concluded that supplemental dietary biotin should not be less than 0·21?mg/kg to minimise the incidence of FPD.     

拉萨白鸡鸡蛋营养成分的分析

为了研究拉萨白鸡鸡蛋的营养成分,随机抽取43周龄的拉萨白鸡鸡蛋40枚,进行营养成分测定。结果表明：拉萨白鸡鸡蛋水分含量为72.50 g/100 g;蛋白质含量为13.00 g/100 g;脂肪含量为9.87 g/100 g;磷脂含量为5.47×10~4 mg/kg;胆固醇含量为2.59×102 mg/100 g;16种氨基酸总含量为11.96 g/100 g,且在必需氨基酸中,亮氨酸的含量最高,为0.98 g/100 g;10种脂肪酸总含量为96.20%,其中C14∶0占0.35%,C16∶0占25.74%,C16∶1占2.21%,C17∶0占0.20%,C18∶0占9.87%,C18∶1占37.32%,C18∶2占19.18%,C18∶3占0.44%,C19∶0占0.19%,C22∶6占0.70%;9种矿物元素含量锌为12.70 mg/kg,铜为0.63mg/kg,铁为22.30 mg/kg,镁为1.04×10~2 mg/kg,锰为0.35 mg/kg,钾为1.35×10~3 mg/kg,钠为1.34×10~3 mg/kg,钙为6.30×10~2mg/kg,磷为2.08×10~3 mg/kg。     

Evaluation of cimaterol for finishing swine including a drug withdrawal period

One hundred fifty crossbred pigs (55 kg) were allotted by weight, sex and litter to a randomized complete-block design with five dietary treatments, six blocks per treatment and five pigs per pen with sex equalized across treatments. Corn-soybean meal-based diets (.65% lysine) with 0, .25 and .5 mg/kg cimaterol were fed, on an ad libitum basis, to pigs slaughtered at an average pen weight of 104 kg/pig. Drug withdrawal prior to slaughter was 1, 3 and 5 d for pigs fed cimaterol at .25 mg/kg and 1 d for those fed cimaterol at .5 mg/kg of diet. Dietary cimaterol level influenced (quadratic, P less than .01) average daily gain during the first 42 d on test; however, daily feed intake and feed conversion ratio were not affected (P greater than .1). Pigs fed .25 mg/kg cimaterol with a 1-d drug withdrawal had 6.8, 7.7 and 13.5% less 10th rib fat depth and 11.1, 6.1 and 13.3% less P2 fat depth than those subjected to either a 3- or 5-d drug withdrawal or those fed the 0 mg/kg cimaterol diet (control), respectively. Overall, pigs fed cimaterol had 7.9% larger longissimus muscle area and 2.6% more kilograms of muscle than pigs fed the control diet. Cimaterol fed at .5 mg/kg resulted in higher (P less than .05) Warner-Bratzler shear force values and altered the proportion of saturation in some long-chain fatty acids, although the total saturated:unsaturated fat ratio was not affected. Pigs fed no cimaterol had less thaw loss (P less than .05) than did those fed other treatments.(ABSTRACT TRUNCATED AT 250 WORDS)     

Pharmacokinetics of levofloxacin in Japanese quails (Coturnix japonica) following intravenous and oral administration

1. The pharmacokinetics of levofloxacin were investigated in Japanese quails after a single dose of 10?mg/kg BW, given either intravenously or orally.

2. Following intravenous administration, the mean value of distribution at steady state (Vd_ss), total body clearance (Cl_tot) and mean residence time (MRT) of levofloxacin were 1·25?l/kg, 0·39?l/h/kg and 2·72?h, respectively.

3. Following oral administration of levofloxacin, the peak plasma concentration (C_max) was 3·31?µg/ml and was achieved at a maximum time (T_max) of 2?h. Mean residence time (MRT), mean absorption time (MAT) and bioavailability were 4·26?h, 1·54?h and 69·01%, respectively. In vitro plasma protein binding of levofloxacin was 23·52%.

4. Based on pharmacokinetic and pharmacodynamic integration, an oral dose of 10?mg/kg levofloxacin for every 12?h is recommended for a successful clinical effect in quails.     

Effects of diet acidification and xylanase supplementation on performance, nutrient digestibility, duodenal histology and gut microflora of broilers fed wheat based diet

1. The objective of this experiment was to study the influences of xylanase and citric acid on the performance, nutrient digestibility, digesta viscosity, duodenal histology, and gut microflora of broilers fed on a wheat based diet.

2. The experiment was carried out as a 2?×?3 factorial arrangement with two concentrations of xylanase (0 and 200?mg/kg) and three concentrations of citric acid (0, 20 and 40?g/kg). A total of 408 one-day-old chickens with similar body weight were distributed into 24 pens with 17 birds/pen. Each dietary treatment was given to 4 replicate pens from 0 to 24?d of age. To determine the apparent nutrient digestibility, chromic oxide (3?g/kg) was added to the diets as an indigestible marker.

3. Xylanase significantly increased body weight gain at 24?d of age by 1·4% and improved gain-to-feed (G:F) by 3·6%. The inclusion of 40?g/kg citric acid decreased feed intake and body weight gain by 15·4% and 11·8%, respectively. The inclusion of 20?g/kg of citric acid decreased feed intake, but it did not affect body weight gain of broilers at 24?d of age. The inclusion of 20 and 40?g/kg citric acid improved G:F by 3·8 and 4·3% respectively. Xylanase significantly decreased the viscosity of digesta and improved retention of DM, CP, and energy.

4. Xylanase and citric acid did not have any effect on the histo-morphology of the duodenum and intestinal microbial population.

5. In conclusion, citric acid at 20?g/kg decreased feed intake, did not have a negative effect on body weight gain, and improved G:F. Xylanase decreased digesta viscosity, increased nutrient retention and consequently improved performance of broilers fed on a wheat based diet.     

Pharmacokinetics of gatifloxacin in broiler chickens following intravenous and oral administration

1. The pharmacokinetics of gatifloxacin were investigated following intravenous and oral administration of a single dose at a rate of 10?mg/kg body weight in broiler chicks.

2. Drug concentration in plasma was determined using High Performance Liquid Chromatography with ultraviolet detection on samples collected at frequent intervals after drug administration.

3. Following intravenous administration, the drug was rapidly distributed (t_1/2α: 0·33?±?0·008?h) and eliminated (t_1/2β: 3·62?±?0·03?h; Cl_B: 0·48?±?0·002?l/h/kg) from the body.

4. After oral administration, the drug was rapidly absorbed (C _max: 1·74?±?0·024?µg/mL; T _max: 2?h) and slowly eliminated (t_1/2β: 3·81?±?0·07?h) from the body. The apparent volume of distribution (V_d(area)), total body clearance (Cl_B) and mean residence time (MRT) were 3·61?±?0·04?l/kg, 0·66?±?0·01?l/h/kg and 7·16?±?0·08?h, respectively. The oral bioavailability of gatifloxacin was 72·96?±?1·10 %.

5. Oral administration of gatifloxacin at 10?mg/kg is likely to be highly efficacious against susceptible bacteria in broiler chickens.