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为了研究辽宁绒山羊精液稀释液对精液低温保存效果的影响,前期试验研制了10个配方,用于绒山羊精液保存,测定了低温保存不同时间的精子活力;根据试验数据,初选了低温保存时间最长、活力最高的精液稀释液配方。以此配方为基础,通过调整三羟甲基氨基甲烷(Tris)、乙二胺四乙酸(EDTA)、柠檬酸(1H2O)的添加剂量,形成了4组精液稀释液配方,进一步测定了精液低温保存不同时间的精子活力,从中确定了效果最优的精液稀释液最终配方:三羟甲基氨基甲烷(Tris) 2.42 g、柠檬酸(1H2O) 1.34 g、乙二胺四乙酸(EDTA) 0.20 g、葡萄糖1.00 g、新鲜卵黄20.0 mL、超纯水80.0 mL、青霉素1.0×105 IU、链霉素1.0×105 IU。以最终配方配制辽宁绒山羊精液稀释液,对绒山羊原精进行稀释,低温保存4 d后进行辽宁绒山羊人工输精,统计了母羊的受胎率。结果表明:以最终配方对辽宁绒山羊精液进行稀释,低温保存到第4天时的精子活力为(66.78±5.12)%,用于配种后母羊受胎率达到91.3... 相似文献
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辽宁绒山羊细管冻精配种受胎率试验 总被引:1,自引:0,他引:1
通过几年的研究,辽宁绒山羊细管冷冻精液质量明显提高.为了检验细管冷冻精液的应用效果.我们在辽宁绒山羊原种场进行了配种受胎试验。 相似文献
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2005年,辽宁省辽宁绒山羊育种中心引进了世界先进的德国米尼图0.25ml细管冷冻精液成套生产设备,进行辽宁绒山羊0.25ml细管冷冻精液工厂化生产。笔者亲历了设备引进、安装、调试、试生产,直至现在工厂化生产。应用米尼图冻精设备对辽宁绒山羊0.25ml细管冷冻精液的质量有明显的提高。 相似文献
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辽宁绒山羊冷冻精液人工授精是一项新技术,经辽宁省畜牧科学研究院的多年研究及在岫岩县的推广应用,取得了较好的效果,对全面快速提高辽宁绒山羊的质量、降低养羊的风险、提高养羊的经济效益起到了巨大的推动作用,目前实际应用的输精方法按输精的部位分为阴道输精、子宫颈输精和子宫角输精3种方法.经过几年的辽宁绒山羊冷冻精液人工授精技术推广研究及试验证明,输精的方法对受胎率有较大的影响. 相似文献
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辽宁绒山羊冷冻精液人工授精技术在辽宁省推广已经是第3年了,经辽宁省辽宁绒山羊育种中心的领导及技术专家的正确指导,经各市(县)区畜牧技术推广工作人员的努力工作,已取得了较好的效果,辽宁绒山羊的质量得到快速的提高. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献