A note on the population structure of the Avileña breed of cattle in Spain

The objective of this study was to describe the population structure of the Avileña breed considering the flow of genes between herds, effective number of herds and generation intervals. Characteristics of the breed which modify either the increase of level of inbreeding or the hierarchical structure of the population were also considered.     

Effect of the El Niño phenomenon on the ovarian responsiveness and embryo production of donor cows

The effects of different Temperature Humidity Index (THI) values in cold, hot and El Ni?o (EN) climates on superovulation and embryo production were analysed on Holstein Friesian donor cows. There were significant differences in the THI among the three climates. The average temperature in the EN period was 6 degrees C higher than in the summer period of the previous 30 years. The number of corpora lutea (CL) and embryos were log- and back-transformed, Kolmogorov-Smirnoff test was used for normality and Lilliefors test was applied for significance. In the cold season THI was 70.74 +/- 1.35 and the average number of CL was 9.84 +/- 4.37. In the hot season the THI was 73.99 +/- 0.72 and the average number of CL was 9.70 +/- 4.49. When the THI, in the EN period, increased up to 79.74 +/- 4.01, the superovulation response was significantly (P < 0.01) reduced (average number of CL = 5.22 +/- 2.53). The embryo production result showed a similar tendency. In the hot period the average number of embryos obtained was 5.87 +/- 2.98. However, in the EN period it decreased to 4.21 +/- 2.05. Higher temperature reduced embryo quality. The proportion of live embryos (%) was 59.2 +/- 37.4 in the cold and 38.2 +/- 38.5 in the EN periods of the year (P < 0.01). However, ovarian sensitiveness showed adaptation to summer environment while the heat stress, which was more severe in the EN period, negatively affected the superovulation response and embryo production.     

Muturu cattle breed management in the smallholder farming area of the Ouémé district in the south of Benin: rearing practices,objectives, and constraints

Tropical Animal Health and Production - An exploratory survey was conducted to understand breeding practices and breeding objectives as well as the constraints as a first step towards developing...     

The Agersoe cattle: the last remnants of the Danish island cattle (Bos taurus)?

A phenotypically interesting strain of cattle existed on the small island of Agersoe, on the west coast of Zealand, Denmark, in the beginning of the last decade. The cattle share a great resemblance to the extinct Danish breed, the Island cattle. The objective of this study was to genetically characterize the Agersoe cattle, using microsatellites, amplified fragment length polymorphism (AFLP) and mtDNA markers, and analyse the genetic variability within the breed and the genetic relationship to 14 European breeds with focus on the Red Danish and Jutland breed. The results show diversity in nuclear markers comparable to that of modern breeds and that the Agersoe cattle are separable from the two native breeds. The absence of inbreeding and the degree of genetic diversity are taken as a sign of recent admixture. The Agersoe cattle did not exhibit a consistent association with any of the European breeds. Several arguments based on this survey have been put forward in favour of characterizing the Agersoe cattle as being the last remnants of the Danish Island Cattle.     

Milk yield estimation of Ogaden cattle breed based on methods of weigh–suckle–weigh and calves’ growth

Daily milk yield of Ogaden cattle was estimated from milk suckled by 269 calves born and maintained between 1994 and 2004 at Haramaya University Beef Farm, Ethiopia. The weigh–suckle–weigh method and the growth of calves were used to estimate the yield. Mean predicted daily milk yield of the breed based on the weigh–suckle–weigh method was 4.39 ± 0.03 kg day⁻¹. Daily milk yield estimated from the growth of calves up to 3 months of age was 4.59 ± 1.43 kg day⁻¹. The milk yield was significantly affected by seasons of calving (p < 0.001), parities of the dams (p < 0.05), weeks of lactation (p < 0.001) and birth weights of calves (p < 0.05). A relatively higher milk yield was observed from cows calved during the long rain season. A higher milk yield was produced by cows in fifth parity. Heavier calves at birth suckled more milk than lighter calves. Daily milk yield was significantly higher in 3–6 weeks of lactation. The result of this study indicated that the milk yield produced by Ogaden breed was relatively higher than the yield of some Zebu breed measured by partial suckling system in Ethiopia and was comparable to the yield from Zebu cattle in the tropics estimated by the same method.     

Effect of prolonging the non‐productive period on the performance of moulted broiler breeders

1. The effect of prolonging the non‐productive period by offering cracked grain after food withdrawal on the performance of moulted broiler breeders was investigated.

2. Each increase in the non‐productive period resulted in a gradual decrease in egg production but a gradual increase in the percentages of hatching eggs, fertility and hatchability.

3. There were no significant differences between hens fed on cracked grain for 2 or 3 weeks for any of the performance characteristics, except fertility and hatchability. The best fertility and hatchability were observed in hens fed on cracked grain for 3 weeks.     

Effects of breed type and age at first calving on length of productive life in a cow–calf system in Southeastern Mexico

The effect of age at first calving (AFC) and breed type (BG) on cow length of productive life in the herd (LPL) and cumulative kilograms of calves weaned per cow (KWC) was studied. Data on 313 Brahman, 183 Nellore, and 269 crossbred cows (undefined crosses of European × Zebu cattle) born from 1986 to 2002 were used. Three hundred and ninety-one out of the 765 cows had non-censored data. LPL curves by BG and AFC (<2.5, 2.5 to <3, 3 to <3.5, and ≥3.5 years of age) were obtained using life table procedures. The magnitude of the effect of AFC and BG on LPL was determined using Cox proportional hazard regression procedures. KWC data (n = 359) were analyzed by a linear model that included the effects of period of birth of the cow (in years), BG, and AFC. There was no difference in the survival curves between BG (p > 0.05), but there was for the hazard ratio of culling (p < 0.05). Brahman and Nellore cows produced more kilograms of calves (p < 0.05) than crossbred cows. AFC influenced LPL (p = 0.014) and KWC (p = 0.012). Cows with AFC <3 years of age stayed longer in the herd and weaned more KWC than those with a higher AFC (p < 0.05).     

Evaluation Modeling of the Best College Coaches

We construct several models to select the best five coaches in different files at college for the previous century.Frist of all,aiming at the college coaches’major diathesis,a scientific questionnaire is built up.we select reasonably four aspects:ability,career,personal achievement,matches played,to compare coaches’achievement by predigesting the integrated evaluation index system based on the online data from NCAA[1].Finally,we get the rough relative weight of the four aspects.Then we study the accurate weight.First,we use AHP to get more accurate weight at the foundation of the rough relative weight.However,the weight is slightly subjective.Therefore,we filter the collected data,and employ Entropy Method to get weight.Finally,the Meta Synthesis Weight Approach is stipulated to combine the two methods and get the ultimate weight.In the end,we calculate the score every coach get and obtains the ranking..Then,we calculate the scores in weighted way based on the weight of Model.     

Validation of the Italian version of the UNESP–Botucatu unidimensional composite pain scale for the assessment of postoperative pain in cattle

Objective

The aim of this study was to validate the Italian version of the UNESP–Botucatu Unidimensional Composite Pain Scale (UCPS-IV) for assessing postoperative pain in cattle.

An outbreak of East Coast Fever on the Comoros: A consequence of the import of immunised cattle from Tanzania?

In 2003 and 2004, a severe epidemic decimated the cattle population on Grand Comore, the largest island of the Union of Comoros. Fatalities started soon after the import of cattle from Tanzania. Theileria parva and its vector, Rhipicephalus appendiculatus, could be identified as the main culprits of the epidemic. Characterisation by multilocus genotyping revealed that the T. parva parasites isolated on the Comoros were identical to the components of the Muguga cocktail vaccine used in Tanzania to immunise cattle. Therefore, it is believed that East Coast Fever reached the Comoros while some of the imported livestock got infected in Tanzania by ticks of which the immature stadia fed on Muguga cocktail vaccinated animals. Since the Comorian government neither has the financial means nor the competent staff to pursue an adequate epidemiosurveillance, the danger exists that without external assistance and in a context of continuing globalisation more transboundary diseases will affect the Comorian livestock sector in the future.     

Molecular and phenotypic characteristics of CMY-2 β-lactamase-producing Salmonella enterica serovar Typhimurium isolated from cattle in Japan

Isolates of extended-spectrum cephalosporin (ESC)-resistant Salmonella enterica serovar Typhimurium obtained from two different farms in Fukushima Prefecture, Japan, in 2007 were characterized in order to determine the genetic basis of resistance. ESC resistance in the two isolates was mediated by an AmpC β-lactamase encoded by the bla(CMY-2) gene, which is located in a large self-transmissible plasmid in each isolate. The sizes of the bla(CMY-2)-carrying plasmids were different. The replicon types of the plasmids were I1-Iγ and A/C. The results of macrorestriction analysis and phage typing suggest a close relationship between both isolates. This is the first report of ESC-resistant S. Typhimurium isolated from cattle in Japan.     

Evaluation of the ocular penetration of topical alpha-luminol (Galavit®/GVT®)

Purpose Oxidative stress plays a major role in the pathogenesis of many neurodegenerative diseases. It has also been implicated as part of the pathogenic mechanisms in the development of glaucoma. Alpha‐luminol has shown profound anti‐inflammatory and antioxidant effects in both experimental animal and human clinical studies. The purpose of this pilot study was to investigate for the first time the ocular penetration of topical alpha‐luminol. Methods Nine animals were divided into three treated groups (three animals each; one drop OU/n = 18), each group receiving a different concentration of the eyedrop (0.5%, 1.5%, 2.5%). Aqueous humor and peripheral blood samples were obtained from each rabbit at three different timepoints (20 min, 4 h and 12 h). Samples were analyzed by means of high performance liquid chromatography and mass spectrometry; median values were compared. Results Alpha‐luminol was found in the aqueous humor in all treated groups at all timepoints. At the 2nd and 3rd timepoints (4 h and 12 h), aqueous humor levels decreased significantly (P < 0.05) for two of the three dosages tested and it was not detectable in some eyes. The highest aqueous humor concentration of the drug was 272 ng/mL after 20 min (0.0217% of one drop, 2.5% group). Alpha‐luminol was found in the vitreous in two animals, one in the 1.5% and another in the 2.5% group (16.4 and 21.5 ng/mL, respectively), at 12 h. Conclusions Topically administered alpha‐luminol readily penetrates into the anterior chamber and can penetrate into the vitreous chamber. Further investigation is warranted to better understand the intraocular pharmacokinetics of alpha‐luminol.     

Genome-wide scan for runs of homozygosity in the composite Montana Tropical® beef cattle

The aim of this study was to assess the distribution of runs of homozygosity (ROH) and autozygosity islands in the composite Montana Tropical^® beef cattle to explore hotspot regions which could better characterize the different biological types within the composite breed. Montana animals (n = 1,436) were genotyped with the GGP-LD BeadChip (~30,000 markers). ROH was identified in every individual using the plink v1.90 software. Medium and long ROH prevailed in the genome, which accounted for approximately 74% of all ROH detected. On an average, 2.0% of the genome was within ROH, agreeing with the pedigree-based inbreeding coefficient. The Montana cattle with a higher proportion of productive breed types showed the highest number of autozygosity islands (n = 17), followed by those with a higher proportion of breeds adapted to tropical environments (n = 15). Enriched terms (p < .05) associated with the immune and inflammatory response, homeostasis, reproduction, mineral absorption, and lipid metabolism were described within the autozygosity islands. In this regard, over-represented GO terms and KEGG pathways described in this population may play a key role in providing information to explore the genetic and biological mechanisms together with the genomic regions underlying each biological type that favoured their optimal performance ability in tropical and subtropical regions.     

Intestinal parasitism in the animals of the zoological garden "Peña Escrita" (Almuñecar, Spain)

Gastrointestinal parasites cause serious diarrhoea in captive animals. Therefore, we have undertaken this study to establish programmes to prevent, control, and treat intestinal parasitism in the animals of the zoological garden "Pe?a Escrita" of Almu?ecar (Granada). An annual survey was conduced to estimate the occurrence of gastrointestinal parasites and the seasonality of this parasitism. Between June 2006 and May 2007, 432 samples were collected from primates, carnivores, perissoodactyla, artiodactyla, rodentia, diprotodontia, galliformes, anseriformes and struthioniformes. One or more intestinal parasites were identified in 72.5% of the animals. The most frequent pathogenic endoparasites were Eimeria spp. (17.3%), Trichuris spp. (5.1%), Strongyloides spp. (4.5%), Cyclospora spp. (4.5%), Cryptosporidium spp. (3.2%) and Isospora spp. (2.6%). Iodamoeba butschlii, Parascaris equorum and Trichuris spp. did not vary with season and Cryptosporidium spp., Dicrocoelium dendriticum, Metastrongylus spp. and Cylicospirura spp. appeared exclusively in Artiodactyla. Multiple parasitic infections were common, 70% of animals presented with at least two parasites (maximum=6). The most frequent cases of multiple parasitism were Eimeria spp. plus Blastocystis spp. and Eimeria spp. plus Nematodirus spp., in the last case the animals presented explosive diarrhoea. In accord with our results, after each sampling, some of the affected animals were treated and the corresponding programmes of prevention and control were designed.     

Characteristics of Nutrient Contents and Evaluation of Nutritional Value of the Alpine Grassland Plants in the Source Area of the Yellow River北大核心CSCD

In order to explore the characteristics of nutrient contents and scientifically evaluate the nutritional value of the alpine grassland plants in the source area of the Yellow River, the plants of the five different alpine grasslands were selected as the research objects, and the content of crude protein (CP), ether extract (EE), crude fiber (CF), crude ash (CA), total phosphorus (TP) of the plants were determined. At the same time, the principal component analysis method was used for quantitative comprehensive evaluation of the plants nutritional value. The results showed that the CP content of the plants in all alpine grasslands was between 12.78%~15.35%, the EE content was between 2.63%~3.38%, the CF content was between 17.56%~25.16%, the CA content was between 5.52%~11.53% and the TP content was between 0.11%~0.27%. The order of nutrient value of plants in different alpine grassland from high to low was: degraded alpine meadow > native alpine meadow > swamp meadow > degraded alpine steppe> native alpine steppe;The order of yield per unit area of the total nutrients of plants in different alpine grasslands from high to low was: swamp meadow > native alpine meadow > native alpine steppe> degraded alpine steppe> degraded alpine meadow;The order of nutrient value of different plant species from high to low was: Leguminosae>Forbs >Cyperaceae>Gramineae. © 2019 Authors. All rights reserved.     

Phenotypic–genotypic resistance in Salmonella spp. isolated from cattle carcasses from the north central zone of the State of Mexico

Salmonella is a public and animal health problem due to the generation of strains multiresistant to antimicrobial products. The objective of this study was to determine prevalence and antimicrobial phenotypic and genotypic resistance of Salmonella spp. isolated from beef cattle carcasses killed in slaughterhouses of the north central zone of the State of Mexico. Sampling was carried out according to the European Directive 2001/471/EC; isolation and identification of the strain was carried out according to the Mexican Official Standard NOM-114-SSA1-1994; resistance was established by CMI according to the National Committees for Clinical Laboratory Standards (NCLS) and multiplex PCR according to Ahmed et al. (Journal of Applied Microbiology 106:402–409, 2009) with PSE-1, tetG, qnrS, FloR, STR, and sul1 oligonucleotides. Twenty-seven strains of Salmonella spp. were obtained from 327 samples (prevalence of 0.083); 19 strains (70 %) were resistant to 10 μg/ml of ampicillin, 15 of these (79 %) had the PSE-1 gene; 22 strains (84 %) were resistant to 30 μg/ml streptomycin, 14 of these (63.6 %) had the STR gene. Genes PSE-1 and STR were factors in the presence of resistance, the rest of the genes (tetG, qnrS, FloR, and sul1) were not factors of resistance in the studied strains.     

Expressed gene sequence of the IFNγ-response chemokine CXCL9 of cattle, horses, and swine

This report describes the cloning and characterization of expressed gene sequences of bovine, equine, and swine CXCL9 from RNA obtained from peripheral blood mononuclear cells (PBMC) and other tissues. The bovine coding region was 378 nucleotides in length, while the equine and swine coding regions were 381 nucleotides. Mapping showed that all three sequences were coded for in four exons in the genome, as are the human and mouse genes. The bovine, equine, and swine coding regions shared 83%, 86%, and 84% homology with human CXCL9, respectively, and all three were 74% homologous with mouse CXCL9. Cladogram comparison of the nucleotide sequences of CXCL9 showed that the bovine, equine and swine sequences were more closely related to one another than to either the human or the mouse sequences. However, the human sequence was more closely related to them than it was to the mouse sequence. These relationships were preserved when the deduced amino acid sequences were evaluated and all sequences showed conservation of the characteristic four cysteines. This work sets the stage for further work with these molecules; an integral goal of the U.S. Veterinary Immune Reagent Network is to develop reagents for investigating diseases in livestock species, poultry, and fish.