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鸡传染性支气管炎是严重危害养鸡业的疾病之一,在广东对本病的主要兽医防疫措施是接种H株或D株疫苗。本文对这两株疫苗及其应用效果进行比较,现将结果报告如下。1试验材料1.1鸡传染性支气管炎疫苗病毒(IBV)H120株(自中国兽药监察所)、D41株(自广东兽医研究所)和B1系新城疫毒(自南京药械厂),种毒原液均10倍稀释后经尿囊腔接种SPF鸡胚0.2ml/只,37℃培养36小时,收取尿囊液备用。1.2鸡胚蛋购自中国兽药监察所(SPF蛋)和仲凯农技学院(非免疫蛋)。1.3试验小鸡为非免疫1日龄石歧杂黄鸡,购自广州市郊。2方法和结果2.1… 相似文献
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传染性法氏囊病病毒抗原的培养提纯及其单克隆抗体的特性研究 总被引:1,自引:0,他引:1
在制备传染性法氏囊病病毒(IBDV)单克隆抗体前,首先要提纯IBDV抗原,然后才能免疫BALB/c小鼠,检测和筛选阳性孔,克隆阳性孔并制备单克隆抗体。本试验采用蔗糖密度梯度离心法和超滤膜超滤浓缩法提纯IBDV,并以纯化抗原免疫BALB/c小鼠,成功地制备出抗IBDV单克隆抗体。1 材料和方法1.1 材料IBDVD78、NDV、IBV和MDV均由哈兽研生物技术国家重点实验室提供;SPF鸡胚,购自黑龙江省生物制品一厂。1.2 鸡胚成纤维原代细胞制备按Hanson等描述方法制备鸡胚成纤维细胞。1.3 病毒繁殖将IBDVD78以0.2%的剂量接种在已长成单层的鸡… 相似文献
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应用病毒感染的鸡胚材料免疫兔的方法制备抗鸡传染性支气管炎病毒(IBV)单因子血清,然后在鸡胚上对山西分离的6个毒株和6个参考株进行交叉病毒中和试验。结果显示,这6株病毒与参考毒株不属于同种血清型,但分离株之间存在部分交叉免疫保护,证实了鸡传染性支气管炎病毒毒株在山西地区存在变异。 相似文献
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鸡传染性法氏囊病活疫苗在对鸡的传染性法氏囊病免疫上广泛应用 ,其特点是使用方便 ,免疫效果好。但在其效力检验上 ,我国目前使用的方法与国外一些国家使用的方法存在着差异。为此我们对鸡传染性法氏囊病活疫苗进行了效力检验对比试验 ,现报告如下。1 试验材料SPF鸡胚 :由黑龙江化血研生物技术有限公司SPF鸡场提供SPF种蛋 ,本实验室自行孵化。鸡胚成纤维细胞 :由SPF胚自行制备的鸡胚成纤维细胞(CEF)。鸡传染性法氏囊病活疫苗 :由黑龙江省生物制品一厂提供(批号 :2 0 0 0 5 2 ) ,每瓶 10 0 0羽份。由某合资公司提供 (批号 :… 相似文献
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在制备传染性法氏囊病病毒 (IBDV)单克隆抗体前 ,首先要提纯IBDV抗原 ,然后才能免疫BALB/c小鼠 ,检测和筛选阳性孔 ,克隆阳性孔并制备单克隆抗体。本实验采用蔗糖密度梯度离心法和超滤膜超滤浓缩法提纯IBDV ,并以纯化抗原免疫BALB/c小鼠 ,成功地制备出抗IBDV单克隆抗体。1 材料和方法1.1 材料IBDVD78、NDV、IBV和MDV均由哈兽研生物技术国家重点实验室提供 ;SPF鸡胚 ,购自黑龙江省生物制品一厂。1.2 鸡胚成纤维原代细胞制备按Hanson[1] 等描述方法制备鸡胚成纤维细胞。1.3 病毒繁殖将… 相似文献
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IB一度成为鸡继新城疫后的主要传染病之一 ,并且因为传染性支气管炎病毒 (IBV)能引起鸡的呼吸道型、肾型、腺胃型等病变 ,血清型众多 ,相互之间交叉免疫性差 ,给本病的防治带来了很大困难。珍珠鸡抵抗力较强 ,传染病的发生比鸡、山鸡少而轻 ,但养殖者往往过高估计了珍珠鸡的抵抗力 ,对其采取的免疫等预防措施不够 ,导致了一定的损失。本文报道 3起珍珠鸡IB疫情的发病与诊断情况 ,以引起广大养殖者的重视。1 发病情况与解剖变化2 0 0 0年 5月 ,兰州市红古区某珍珠鸡养殖场饲养的 110 0只珍珠鸡发病 ,其中 1月龄 60 0只 (A1群 ) ,2月龄 5… 相似文献
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为了提高鸡传染性支气管炎病的治疗效果,试验用中药制剂复方板蓝根进行鸡胚感染试验,结果显示,此制剂对胚胎的发育无任何不良影响,且不同浓度的药液对感染鸡胚均有保护作用.因此,复方板蓝根制剂具有明显抗传染性支气管炎病毒的作用. 1材料与方法 相似文献
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鸡新城疫、传染性支气管炎、传染性法氏囊病三联弱毒疫苗的试制 总被引:1,自引:0,他引:1
用同胚培养的鸡新城疫Lasota株和传染性支气管炎H120(或H52)株以及用鸡胚成纤维细胞增殖的传染性法氏囊弱毒株以适当比例混合为抗原,用蔗糖脱脂乳作保护剂,经真空冷冻干燥制成三联弱毒疫苗。通过三批疫苗室内外各项指标的测试,表明该三联疫苗安全性能可靠,免疫效果确实,使用方法简便。在实验室进行的物理性状检验、无茵检验、支原体检验、剩余水分检验、真空度检查均符合国家标准;用10倍大剂量接种15日龄雏鸡无不良反应;以常规量颈部皮下接种免疫后,7d产生免疫力,免疫后14d抗鸡新城疫、鸡传染性支气管炎、鸡传染性法氏囊病三株强毒攻击的保护率均为,100%。对15日龄雏鸡首免,其免疫期至少为30d,在-25℃保存期为1年。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2%的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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