Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia

Dry bean (Phaseolus vulgaris L.) is an important legume worldwide and nitrogen (N) is most yield limiting nutrients. A field experiment was conducted for two consecutive years to evaluate response of 15 dry bean genotypes to nitrogen and rhizobial inoculation. The N and rhizobia treatments were (i) control (0 kg N ha^?1), (ii) seed inoculation with rhizobia strains, (iii) seed inoculation with rhizobia strains + 50 kg N ha^?1, and (iv) 120 kg N ha^?1. Straw yield, grain yield, and yield components were significantly influenced by N and rhizobial treatments. Grain yield, straw yield, number of pods m^?2, and grain harvest index were significantly influenced by year, nitrogen + rhizobium, and genotype treatments. Year × Nitrogen + rhizobium × genotype interactions were also significant for these traits. Hence, these traits varied among genotypes with the variation in year and nitrogen + rhizobium treatments. Inoculation with rhizobium alone did not produce maximum yield and fertilizer N is required in combination with inoculation. Based on grain yield efficiency index, genotypes were classified as efficient, moderately efficient, and inefficient in nitrogen use efficiency (NUE). NUE defined as grain produced per unit N applied decreased with increasing N rate. Overall, NUE was 23.17 kg grain yield kg^?1 N applied at 50 kg N ha^?1 and 13.33 kg grain per kg N applied at 120 kg N ha^?1.     

Nitrogen Leaching Of Spring Wheat Genotypes (Triticum Aestivum L.) Varying In Nitrogen-Related Traits

Efficient use of nitrogen (N) by wheat crop and hence prevention of possible contamination of ground and surface waters by nitrates has aroused environmental concerns. The present study was conducted in drainage lysimeters for three years (1998–2000) to identify whether spring wheat genotypes (Triticum aestivum L.) that differ in N-related traits differ in N leaching and to relate parameters of N use efficiency (NUE) with parameters of N leaching. For this reason two spring wheat cultivars (‘Albis’ and ‘Toronit’) and an experimental line (‘L94491’) were grown under low (20 kg N ha^?1) and ample N supply (270 kg N ha^?1). The genotypes varied in parameters of NUE but not in N leaching. Grain yield of the high-protein line (‘L94491’) was, on average, 11% lower than that of ‘Toronit’ but among genotypes had significantly higher N in the grain (%), grain N yield, and N harvest index. Nitrogen lost through leaching was considerably low (0.42–0.52 g m^?2) mainly due to low volume of percolating water or the ability of the genotypes to efficiently exploit soil mineral N. There were no clear relationships between N-related genotype traits and N leaching, but across all treatments significantly negative correlations between volume of leachate and the amount of N in the total biomass and grain N yield existed.     

COURSE OF DRY MATTER AND NITROGEN ACCUMULATION OF SPRING WHEAT GENOTYPES KNOWN TO VARY IN PARAMETERS OF NITROGEN USE EFFICIENCY

Field experiments were conducted for two years to compare and identify bread spring wheat (Triticum aestivum L.) genotypes which make the most efficient use of nitrogen (N). Such information is required for breeding strategies to reverse the negative relationship between yield and protein content. Three Swiss spring wheat cultivars (‘Albis’, ‘Toronit’, ‘Pizol’) and an experimental line (‘L94491’) were grown without (N0; 0 kg N ha^?1) and with high fertilizer N [(NH₄NO₃); (N1; 250 kg N ha^?1) supply on a clay loam soil with low organic matter content. Biomass and nitrogen accumulation in biomass as well as the leaf growth and senescence patterns (SPAD) were investigated in an attempt to explain the physiology of growth and N translocation of these genotypes. The pre-anthesis accumulation of biomass and N in the biomass depended on genotype only at N1 in 2000. In this year, conditions were less favorable for the pre-anthesis accumulation of biomass and N, which was, on average, 10 and 20% lower, respectively, of the total than in 1999. The contribution of pre-anthesis assimilates to the grain yield (CPAY) was higher in 1999 for all genotypes (36.9%) compared to 2000 (13.5%) except ‘Toronit’. Between anthesis and maturity the climate influenced the genetic variability of some N use efficiency components: N translocation efficiency (NTE) and dry matter translocation efficiency (DMTE). NTE was higher in 1999 (68.1%) compared to 2000 (50.7%); 1999 was a year in which the post-anthesis period was drier and warmer than usual. ‘Toronit’ produced the highest biomass by maturity due mainly to greater and longer lasting green leaf area after anthesis. ‘Albis’ performed relatively well under low input conditions, with considerable amounts of N being re-translocated to the seeds at maturity (NHI), whereas ‘Pizol’ accumulated in grains N as high as for ‘L94491’. In a humid temperate climate breeding for greater N uptake and partitioning efficiency may be a promising way to minimize N losses and produce high phytomass and grain yields. Using high protein lines as selection material and combining them with high biomass genotypes may lead to high protein contents without decreasing yield.     

Response of nitrogen use efficient sorghums to nitrogen fertilizer

Little information is available on the response of grain sorghum [Sorghum bicolor (L.) Moench] genotypes differing in nitrogen (N) use efficiency (NUE) (g DM g N^‐1) to added N fertilizer. Such knowledge is important for reducing the reliance upon fertilizer N. A dryland field experiment was conducted in 1993 and 1994 at Mead, NE evaluating the agronomic responsiveness of 13 sorghum genotypes differing in NUE to three N rates (0, 50 and 100 kg N ha^‐1) and also to determine physiological factors that contribute to improved NUE. The experiment was conducted on a fine montmorillonitic, mesic, Typic Argiudoll soil. Total N at maturity, dry matter, and grain yield were used to calculate NUE terms. Genotype differences were found for all measured variables both years, but no N rate by genotype effects were significant. Nitrogen fertilizer enhanced plant N contents and grain yield, but decreased NUE for total biomass and grain production. An early freeze in 1993 markedly reduced the later maturing genotype grain yields which, in turn, influenced NUE group comparisons. All genotypes in the study attained their full yield potential in 1994. The linear response to N rate of the N non‐responsive group was significantly less than the moderately responsive or N responsive group. High NUE sorghums had greater yields than low NUE types averaged over N levels only in 1994 since Naga White, a high NUE type, did not reach maturity in 1993. There was no difference in the linear response to N between these two groups. A linear increase in grain yield with increasing N rate was significantly greater for hybrids than lines. The results suggest that specific selection for high NUE sorghums will not diminish responsiveness to applied N.     

Nitrogen topdressing at the jointing stage affects the nutrient accumulation and translocation in rainfed waxy maize

Timely and fitting nitrogen (N) application decreases costs and pollution risk in maize cultivation. To explore the accumulation and remobilization of dry matter (DM), N, phosphorus (P), and potassium (K) in waxy maize under various N topdressings (0?kg ha^?1, LN; 150?kg ha^?1, MN; 300?kg ha^?1, HN) at the jointing stage, a field trial involving two waxy maize varieties (Suyunuo 5 and Yunuo 7) was conducted in 2013–2016. The highest grain yield was obtained under MN mainly due to the highest grain numbers and grain weight. The increase in grain yield under MN was mainly due to the high DM accumulation post-silking, as well as high N, P, and K accumulation and remobilization pre-silking. Generally, the plants had high harvest index (HI) of DM (N, P, and K), partial N fertilizer productivity, and moderate N utilization efficiency (NUE) under MN.     

Liming and Nitrogen Effects on Maize Yield and Nitrogen Use Efficiency

ABSTRACT

The study was aimed to determine the appropriate nitrogen (N) rate to combine with liming for enhanced maize yield and nitrogen use efficiency (NUE). Two maize varieties [Ikom White (IKW) and Obatanpa-98 (Oba-98)], two lime rates (0 kg ha^?1 and 500 kg ha^?1) and three N rates (0, 90 and 180 kg ha^?1) were used. The treatments were laid as a split-split plot in a randomized complete block design with three replications. The growth attributes, photosynthetically active radiation (PAR), harvest index, dry matter, and grain yield increased (P ≤ 0.05) with increases in N rates, especially in plots amended with lime. Oba-98 was better yielding (2.12 versus (vs) 1.88 t ha^?1) and absorbed more (P ≤ 0.05) radiation (442.06 vs 409.54 μmol m^?2s^?1) than IKW. The efficiency indices and partial factor productivity were best optimized at the 90 kg ha^?1 N rate with Oba-98 having higher values than IKW. Therefore, liming (500 kg ha^?1) plus N at 180 kg ha^?1produced the best yield of the hybrid maize, Oba-98.     

Residue,tillage and N-fertilizer rate affected yield and N efficiency in irrigated spring wheat

Yield and nitrogen (N)-content in wheat was studied under applied treatments of crop residues (legume vs. cereal), tillage depths (deep vs. shallow) and N-fertilizer rates (0, 40, 80, 120 and 160 kg ha^?1) at wheat-maize cropping systems. Experiments were conducted at Agronomy Research farm, the University of Agriculture, Peshawar Pakistan, during winter season 2009–2010 and 2010–2011 crop growth seasons. Well-chopped crop residues (5 t ha^?1) on dry matter basis of legume (Vigna unguicuata) and cereal (Zea mays) were applied to soil and subsequently plowed with mold-board plow as deep tillage (DT) and cultivator as shallow tillage (ST) treatment (main plot treatments). A month after residue and tillage application, seedbed was prepared and wheat was planted with drill in rows 25 cm apart in middle of November each year. Phosphorus and potassium were applied uniformly 80 and 40 kg ha^?1, respectively during seedbed preparation. N-fertilizer rates were applied in two splits: half 15 days after sowing (DAS) and other half 45 DAS (sub-plot treatment). Uniform cultural practices were applied during crop growth and development. Legumes residues amendments showed better responses than cereal but lower than no-residue treatment for N-content in leaf blade before anthesis (LBA), after anthesis (LAA), straw N-content (SNC), grain N-content (GNC), grain N-uptake (GNU), crop N-removal (CNR), recovery efficiency of added nitrogen (REAN), N-use efficiency (NUE), grain N-uptake (GNU) and grain yield. Likewise, shallow tillage proved better than deep tillage system for LBA, LAA, SNC, GNC, GNU, CNR, REAN, NUE, GNU and grain yield. Increased N-fertilizer from control onwards showed significant (p > 0.05) increments in LBA, LAA, SNC, GNC, GNU, CNR, N-uptake and grain yield. Treatments interaction was also found significant (p > 0.05). Study suggested, regardless of the given treatments, GNU and grain yield were in strong positive linear relationship. Legume residue incorporated shallow out yielded GNU and NUE of spring wheat in wheat-maize cropping system. It is concluded that LR and ST with 120 kg N ha^?1 ensures production of good wheat quantity and quality.     

Effects of Nitrogen Fertilization on Upland Rice based on Pot Experiments

Abstract

Limited information is available regarding the utilization and loss of fertilizer nitrogen (N) applied to intensively managed upland rice. Effects of N fertilization on upland rice were conducted as N0 (no N applied), N225 (225 kg N · ha^?1), N300 (300 kg N · ha^?1), and N375 (375 kg N · ha^?1) in pot experiments. ¹⁵N‐labeled techniques were used in basal and topdressing N fertilizations. Results showed with the increase of N quantity applied, tiller, panicle numbers per pot, and spikelet number per panicle increased significantly (P<0.05). Chlorophyll b content of N225 and N300 were significantly higher than N0 (P<0.05), and net photosynthetic rate (Pn) of N300 increased significantly compared with N0 and N225. Under basal fertilization, N use efficiency (NUE) of root, stem, leaf, and grain in N300 was the highest. The NUE and loss rate ranged from 23.3% to 30.3% and 62.4% to 73.8%, respectively, under basal fertilization. They varied from 16.5% to 27.5% and 70.7% to 80.4%, respectively, under topdressing fertilization. The highest NUE was observed in N300 under basal fertilization. As increased quantities of N were applied, Pn and biological characteristics improved, thus crop yield of upland rice increased. Grain yield of N300 and N375 were significantly higher than that of N0 and N225 (P<0.01); however, there was no significant difference between them. Therefore, N fertilization with medium applied quantity under basal fertilization will facilitate growing, photosynthesis, and grain yield increase of upland rice.     

Nitrogen Use Efficiency and Herbage Production of an Established Grass Sward in Relation to Moisture and Nitrogen Fertilization

Herbage growth and nitrogen (N) use efficiency in grazed or mown grasslands are generally low, associated mostly with poor response to fertilizer N. The aim of the present investigation was to examine the short-term response of grass to fertilizer N with respect to herbage yield and nitrogen use efficiency (NUE) in order to provide a better basis for improving the efficient use of fertilizer N in grassland ecosystems. Both NO₃ ^? and NH₄ ⁺ sources of N were applied to an established grass sward with three moisture levels, i.e., natural conditions (63% water-filled pore space, WFPS), near field capacity level (71% WFPS), and slightly wetter than field capacity (84% WFPS). Herbage yield, i.e., dry matter (DM), N uptake, N recovery efficiency, yield efficiency, and physiological efficiency were determined over a 7–28 d period. Addition of N fertilizers significantly increased the herbage yield and N uptake of grass sward over that of the control. In the plots where NO₃ ^??N was added as the N source, DM yield was between 1760–1870 kg ha^?1, N recovery efficiency was between 24%–43%, and yield and physiological efficiency were in the range of 2.1–3.2 and 6.4–8.8 kg DM kg^{? 1} N, respectively. In NH₄ ⁺?N added plots, the DM yield was between 3190–3700 kg ha^{? 1}, N recovery efficiency was between 39%–48% while yield and physiological efficiency were in the range of 3.5–5.6 and 9.0–11.6 kg DM kg^{? 1} N, respectively. Results indicated that total DM yield, N uptake, and NUE depend on the source of N and the level of moisture in the field. Assimilation of N is also affected by the stage of plant development after N fertilization. About 50%–54% of applied N was recovered in the initial 14 and 21 d after fertilizer application and thereafter translocation of N slowed. A fall in herbage production and minimal response to N fertilizer has been observed at 84% WFPS, while the maximum herbage yield and N recovery efficiency was recorded in soil near or below field capacity. The grass sward with added NH₄ ⁺?N produced a larger yield and had higher NUE relative to the sward with NO₃ ^??N. Results confirm that applied N was not utilized efficiently by grass sward and a decrease in N uptake and its utilization seem to be the key factors responsible for the poor herbage productivity often observed in pastoral agriculture. These results suggest that both moisture and N source have a substantial effect on herbage yield and N utilization by plants and therefore should be considered for efficient management of N fertilization and recommendations for grass sward.     

Field-level comparison of nitrogen rates and application methods on maize yield,grain quality and nitrogen use efficiency in a humid environment

Appropriate nitrogen (N) management practices are of critical importance in improving N use efficiency (NUE), maize (Zea mays) yield and environmental quality. A six-year (2005–2010) on-farm trial was conducted in Ottawa, Canada to assess the effects of N rates and application methods on grain yield and NUE. In four out of the six-year study, grain yield increased by 60–77 kg ha^?1 by sidedress, compared to 49–66 kg ha^?1 for each kg N ha^?1 applied at preplant. Grain yield response to N between the two strategies was similar in the other growing seasons. Sidedress strategy required 15 kg N ha^?1 less of the maximum economic rate of N (MERN) than preplant application. Our results indicate that sidedress application of 90–120 kg N ha^?1 with a starter of 30 kg N ha^?1 resulted in greater yield, grain quality and NUE than preplant N application in this cool, humid and short growing-season region.     

Functional traits associated with nitrogen use efficiency in wheat

The association between functional traits and nitrogen use efficiency (NUE) was investigated to assist the breeding of nitrogen (N) use-efficient bread wheat (Triticum aestivum ssp. aestivum) varieties. This study combined results from a climate chamber experiment involving 41 spring wheat varieties and a field experiment involving six winter and six spring wheat varieties grown with and without the application of mineral N fertiliser. The climate chamber experiment was analysed by partial least squares (PLS) regression, with several predictors and NUE as response, to identify traits related to NUE. Specific hypotheses were then tested in the field experiment. The PLS indicated six traits of particular importance for overall NUE: leaf chlorophyll (SPAD value) of the top leaf at stem elongation, grains ear^?1, ears pot^?1, straw biomass pot^?1, days between emergence and anthesis, and days between emergence and completed senescence. In the field experiment, the SPAD value of flag leaves of winter wheat around anthesis was positively correlated with NUE and total grain N, at both N levels. Fast development was positively correlated with high NUE and N uptake efficiency in spring wheat. Early senescence of the flag leaf was positively correlated with grain N concentration and negatively correlated with grain-specific N efficiency in winter wheat at low N fertilisation levels. The results indicate that high SPAD value of the top leaf might be a candidate trait that could be used in wheat breeding for improved NUE, while genetic variation in senescence could possibly be used to tailor varieties for different end-use quality when grown at low N. More studies are needed to validate these findings in other environments and for other genotypes.     

ENHANCING NITROGEN USE EFFICIENCY BY COMBINATIONS OF NITROGEN APPLICATION AMOUNT AND TIME IN WHEAT

Nitrogen (N) is one of the most important impact factors on development and growth of wheat. In this study the effects of nitrogen use efficiency on quantity and quality of grains were studied by agronomic management of N fertilizers on spring wheat (Triticum aestivum L.) grown under field conditions for two years. The experiments were performed at 16 combinations of N application amount and time, including four levels of N at 0, 60, 120 and 180 kg N ha^?1 that were used as pre-plant fertilizers, sub-treated with four levels of the same N amount used as top-dress fertilizers. As a result, with an increase in total N fertilizers, grain yield increased in a cubic equitation, but partial factor productivity (PFP_N, kg grain yield per kg N applied) decreased exponentially. With total fertilizers, N content and accumulation in vegetative tissues and grains increased linearly, but N uptake efficiency (UtE_N, kg nutrient taken up per kg N applied) decreased exponentially. When N was over-applied (>360 kg N ha^?1 in this study), grain yield clearly declined, due to decrease in productivity from per unit N. The high N level (240～300 kg N ha^?1), the reasonable distribution between pre-plant and top dress from the same amount N fertilizer not only increased grain yield but also enhanced N use efficiency.     

Nitrogen Rate Influence on Pearl Millet Yield,Nitrogen Uptake,and Nitrogen Use Efficiency in Nebraska

Abstract

Pearl millet is a potential dryland crop for Nebraska. Experiments were conducted in eastern Nebraska in 2000, 2001, and 2002, and in western Nebraska in 2000 and 2001. The objectives were to determine optimum nitrogen (N) rate, N uptake, and N use efficiency (NUE) for pearl millet. The hybrids “68×086R” and “293A×086R” and N rates of 0, 45, 90, and 135 kg N ha^?1 were used. Hybrids had similar yield, N uptake and NUE responses. In western Nebraska in 2000, pearl millet yield response to N rate was linear, but the yield increase was only 354 kg ha^?1 to application of 135 kg N ha^?1. In eastern Nebraska, pearl millet response to N rate was quadratic with maximum grain yields of 4040 in 2001 and 4890 kg ha^?1 in 2002 attained with 90 kg N ha^?1. The optimum N rate for pearl millet was 90 kg N ha^?1 for eastern Nebraska. For western Nebraska, drought may often limit pearl millet's response to N fertilizer.     

Evaluation of the Minolta SPAD‐502 chlorophyll meter for nitrogen management in corn

The field study investigated the relationship of Minolta SPAD 502 (SPAD) readings to applied nitrogen (N) fertilizer rate, corn (Zea mays L.) yield, and leaf N concentration. The experiment was conducted on a total of six sites in Illinois during 1991 and 1992. Ten different open pedigree corn hybrids were grown at a final population of 65,000 plants ha^‐1. Nitrogen was applied at four rates(0, 90, 180, and 270 kgN ha^‐1) as 28% liquid N solution. Significant main effects of environment (E), and hybrid (H), and E x H interaction were detected for all measured parameters. SPAD readings and leaf N concentration at all sampling times (V7, R1, and R4) as well as grain N concentration were affected by N fertilizer rate. Maximum mean grain yield and maximum grain N concentration were obtained at 110 and 195 kg N ha^‐1, respectively. At all sampling times the correlation of SPAD readings to N fertilizer rate were low but significant (R=0.22 at V7 and R1, R=0.11 at R4). SPAD correlation to corresponding leaf N concentration improved over time. The Pearson correlation was R=0.33 at V7 and increased to R=0.78 at R4. The SPAD meter did a good job at providing a measure of the relative greenness of living leaves at a specific point in time. Chlorophyll readings can therefore be useful in detecting N deficiencies in growing crops. But, the SPAD meter cannot be used to make accurate predictions of how much fertilizer N will be needed by a crop during the future growing season. We conclude then that the SPAD meter will be most useful as a diagnostic aid rather than a tool for N management in corn.     

Timing and Method of 15Nitrogen-Labeled Fertilizer Application on Grain Protein and Nitrogen Use Efficiency of Spring Wheat

ABSTRACT

Grain protein content is one of the most important quality constraints for bread wheat (Triticum aestivum L.) production in eastern Canada. A field experiment was conducted for two years (1999 and 2000) on the Central Experimental Farm, Ottawa, Canada, to study whether split application of nitrogen (N) fertilizer improved grain protein content and nitrogen-use efficiency (NUE). Two cultivars (‘Celtic,’ as N-responsive and ‘Grandin’, as N-non-responsive) were grown using three different N doses and application methods: (1) 100 kg N ha^?1 as NH₄NO₃, soil-applied at seeding with ¹⁵N₂-labeled NH₄NO₃ to microplots, (2) 60 kg N ha^?1 soil-applied at seeding plus 40 kg N ha^?1 foliar-applied at the boot stage with ¹⁵N₂-labeled urea to microplots, and (3) 90 kg N ha^?1 as soil-applied at seeding plus 10 kg N ha^?1 foliar-applied at the boot stage with ¹⁵N₂-labeled urea to microplots. Plants were sampled at heading and maturity. While dry-matter production and grain yields were not affected by the treatments in either year, N application methods influenced tissue N concentration and NUE. In 1999, extended drought stress led to significant yield reduction; in 2000, foliar application of 10 kg N ha^?1 at the boot stage significantly increased grain N concentration when grain protein was under the limit for bread quality, suggesting that later-applied N can contribute to grain protein content. At maturity, the average NUE was 22.3% in 1999 and 34.5% in 2000, but was always greater when all N was applied at seeding (42.5%) than when N was foliar-applied at the boot stage (18.5% to 24.5%). We conclude that application of a small amount of fertilizer N at the boot stage can improve the bread-making quality of spring wheat by increasing grain protein concentration.     

Nitrogen application in direct wet-seeded rice under alternate wetting and drying irrigation condition: Effects on grain yield,dry matter production,nitrogen uptake and nitrogen use efficiencies

Yield, dry matter production, nitrogen (N) uptake and nitrogen use efficiency (NUE) of Bangladesh Rice Research Institute (BRRI) dhan29 were investigated during two consecutive dry (Boro) seasons of 2009–10 and 2010–11. The experiments were set up in a randomized complete block design with three replication having six nitrogen (N) levels of 0, 40, 80 120, 160 and 200 kg ha^?1. Nitrogen fertilization increased yield characters, dry matter production and N uptake. The economic optimum rate of N was 166 and 155 kg ha^–1 in first and second year, respectively, with corresponding yield of 7.1 and 6.5 t ha^?1. NUEs were higher in the first year, decreased with increasing N rates in most cases. Gross return over fertilizer reached the highest Tk 692 in 2009–10 and Tk 489 in 2010–11 with 160 kg N ha^–1. The results suggest that BRRI dhan29 should receive an average of 160 kg N ha^?1 for economic optimum yield.     

Long-Term Effects of Nitrogen Management Practices on Grain Yield,Nitrogen Uptake,and Efficiency in Irrigated Corn

ABSTRACT

Crop management strategies that improve Nitrogen Use Efficiency (NUE) increase profits while reducing the detrimental effects on the environment associated with fertilizer nitrogen (N) loss. Effective N management should include several critical factors that are very interrelated. A study was conducted at the Panhandle Research and Extension Center, Goodwell, OK to evaluate the effects of multiple nitrogen management practices including N rate, source, time of application, methods of fertilizer and residue incorporation over a long period of time on grain yield, N uptake and NUE in irrigated corn. Fourteen treatments were evaluated in a randomized complete block design with three replicates. Results of data analyzed on the individual year and averages of all years showed that grain yield and N uptake were improved with N rates and N management practices compared to checks. Both N recovery and efficiency of use were high for the 118 kg N ha^{? 1} rate.     

Variable-timing,fixed-rate application of cattle biogas effluent to rice using a leaf color chart: microcosm experiments in Vietnam

ABSTRACT

Livestock production plays a leading role in agricultural land-use change. Producing biogas from livestock waste and subsequently using the biogas effluent as fertilizer for crops is a promising option to solve environmental problems resulting from expanding livestock production. However, it is difficult to promptly and accurately measure the nitrogen (N) concentration of effluent for farmers in developing countries, making precise N management difficult. The objectives of the current study were (1) to evaluate the feasibility of variable-timing, fixed-rate application of cattle biogas effluent using a leaf color chart (LCC) for rice (Oryza sativa L.) and (2) to determine the optimum LCC threshold for grain yield. We conducted two microcosm experiments in the Mekong Delta of Vietnam in 2018 using eight treatments of N-fertilizer application. In the Zero treatment, we applied no N. In the Estd treatment, we split-applied N as effluent (E) at fixed rate and timing as the standard method. In E2.75, E3.00, E3.25, E3.50, and E3.75, we applied effluent whenever the LCC value went below 2.75, 3.00, 3.25, 3.50, and 3.75, respectively. In U3.25, we applied N as urea (U) whenever the LCC value fell below 3.25. The total effluent-N application rate ranged from 90 to 210 kg N ha^?1 season^?1. Rice growth was normal but there was a substantial yield gap between the two microcosm experiments due to the seasonal difference in solar radiation. Rice yield tended to increase with increasing LCC threshold. There was a positive linear relationship between LCC and chlorophyll content (SPAD) values (R ² = 0.73–0.79). Grain yield was well explained (R ² = 0.70–0.89) by the seasonal mean LCC or SPAD value. Plant total N uptake increased with increasing LCC threshold, but the three calculated indices of N use efficiency (NUE) – apparent N recovery, agronomic NUE, and internal NUE – were not always improved with a higher LCC threshold. Our results showed that the tested variable-timing, fixed-rate strategy for the application of cattle biogas effluent was feasible and the optimum LCC threshold for grain production was 3.75 under the current microcosm conditions.     

Effects of Nitrogen Application Rates on Rice Grain Yield,Nitrogen-Use Efficiency,and Water Quality in Paddy Field

Excessive nitrogen (N) fertilizer application is common in the central Zhejiang Province area, China. A three-year (2009–11) experiment was conducted to determine the optimum N application rate for this area by studying the effects of various N rates on rice (Oryza sativa L.) yield, N-use efficiency (NUE), and quality of paddy field water. Results showed that no significant yield differences were observed under N rates from 180 to 315 kg ha^?1. The NUE could be improved by reducing N application rates without significantly decreasing yield. Due to high ammonia (NH₄⁺-N) and nitrate (NO₃^—N) concentrations, 5–7 days after N application was a critical stage for reducing N pollution. The N rate for the greatest yield was 176 kg ha^?1, accounting for 65 percent of the conventional N rate (270 kg ha^?1). The N-rate reduction in this area may be necessary for maintaining high yield, improving NUE, and reducing environmental pollution.     

Using CERES-Maize model to determine the nitrogen fertilization requirements of early maturing maize in the Sudan Savanna of Nigeria

CERES-Maize model was used to determine nitrogen fertilizer requirements of early maturing maize varieties in the Sudan Savanna. Data were collected from 2013 to 2014 field experiments conducted in Bayero University Kano, (BUK), Kano, Nigeria. The experiments consisted of three nitrogen fertilizer levels (0, 60, and 120 kg N ha^?1) and two early maize varieties (EVDT and 2009 TZEEW). Sensitivity analysis was performed to evaluate the responses of the two maizes to N fertilizers and for economic and strategic responses. The model predicted grain yield and harvest index reasonably well for the two varieties. Increasing N application from 0 to 30 kg N ha^?1 increased grain yield by 105%, when nitrogen (N) rate was increased to 60 kg N ha^?1, grain yield increased by 226%. Yield increases of 364%, 451%, and 461% was observed when N rate increased from 0 to 90, 120, and 150 kg ha^?1, respectively.