长期定位试验中氮肥与磷肥有机肥联合施用通过刺激根的生长增加玉米产量和养分吸收

Imbalanced application of nitrogen (N) and phosphorus (P) fertilizers can result in reduced crop yield, low nutrient use efficiency, and high loss of nutrients and soil nitrate nitrogen (NO₃^--N) accumulation decreases when N is applied with P and/or manure; however, the effect of applications of N with P and/or manure on root growth and distribution in the soil profile is not fully understood. The aim of this study was to investigate the combined effects of different N and P fertilizer application rates with or without manure on maize (Zea mays L.) yield, N uptake, root growth, apparent N surplus, Olsen-P concentration, and mineral N (Nmin) accumulation in a fluvo-aquic calcareous soil from a long-term (28-year) experiment. The experiment comprised twelve combinations of chemical N and P fertilizers, either with or without chicken manure, as treatments in four replicates. The yield of maize grain was 82% higher, the N uptake 100% higher, and the Nmin accumulation 39% lower in the treatments with combined N and P in comparison to N fertilizer only. The maize root length density in the 30--60 cm layer was three times greater in the treatments with N and P fertilizers than with N fertilizer only. Manure addition increased maize yield by 50% and N uptake by 43%, and reduced Nmin (mostly NO₃^--N) accumulation in the soil by 46%. The long-term application of manure and P fertilizer resulted in significant increases in soil Olsen-P concentration when no N fertilizer was applied. Manure application reduced the apparent N surplus for all treatments. These results suggest that combined N and P fertilizer applications could enhance maize grain yield and nutrient uptake via stimulating root growth, leading to reduced accumulation of potentially leachable NO₃^--N in soil, and manure application was a practical way to improve degraded soils in China and the rest of the world.     

Effects of Nitrogen Management on Soil Nitrogen Content and Rice Grain Yield in Double Cropping Rice Production Area with Continuous Full Amount of Straw Returning

ABSTRACT

In order to formulate a nitrogen (N) management strategy under continuous full amount of straw returning (CFSR) for double cropping rice production, long-term (2013–2016) paddy field experiments were conducted in double cropping rice production area in the Jiangxi province, China. Five N fertilizer treatments under CFSR were tested, that is, (i) no N fertilizer application (CK); (ii) conventional N fertilizer application (165kg N ha^?1 and 195 kg N ha^?1 in early and late rice variety with the ratio of basal dressing to topdressing as 6:4, respectively) (CNF_6:4); (iii) recommended N fertilizer application (135 kg ha^?1 N and 165 kg ha^?1 N in early and late rice variety with the ratio of basal dressing to topdressing as 4:6, 6:4, and 8:2, respectively) (RNF_4:6, RNF_6:4, and RNF_8:2). Nitrogen fertilizer treatments under CFSR had 5.70% and 8.93% higher soil total nitrogen (TN), 1.32% and 0.80% higher available nitrogen (AN), 16.55% and 22.94% higher NH₄⁺-N, and 13.10% and 7.93% higher NO₃^--N than CK treatments in early and late rice variety, respectively. There were no differences in soil TN, AN, NH₄⁺-N, and NO₃^--N contents between CNF6:4 and RNF6:4 treatments, while CNF6:4 treatment showed higher or significantly higher soil N contents than RNF4:6 and RNF8:2 treatments. N fertilizer treatment under CFSR showed 88.9% and 43.20% higher grain yield and 62.15% and 42.52% higher panicle numbers than CK treatments in early and late rice variety, respectively. Compared with CNF6:4, RNF treatments did not significantly reduce grain yield and yield components in early and late rice variety, respectively, except for RNF8:2. Compared with RNF6:4 and 8:2, RNF4:6 showed higher rice grain yield, while no obvious differences in yield components were obtained among all RNF treatments. We concluded that N fertilizer under CFSR was helpful to improve soil N contents and double rice grain yield and panicle numbers. Appropriate reduction of N application (18% and 15% reduction in early and late rice variety, respectively) on the basis of adjusting ratio of basal dressing to topdressing as 4:6 and 6:4 did not significantly reduce soil TN and double rice grain yield and yield components, especially, the 40% basal N dressing and 60% N topdressing was beneficial to increase double rice grain yield under CFSR.     

Effects of Transgenic Cotton-Based Cropping Systems and Their Fertility Levels on Succeeding Wheat Crop

Cotton–wheat is the second most important cropping system after rice–wheat in India and Pakistan, and is practiced on about 4.02 mha. By 2010, more than 6 million Indian farmers had adopted transgenic Bt cotton on 9.4 mha—almost 90% of the country’s total cotton area. There is a paucity of information on the effects of intercropping and integrated nitrogen (N)–management practices in transgenic Bt cotton on productivity, nutrient availability, and soil biological properties in the succeeding wheat crop in a cotton–wheat system. A study was made to evaluate and quantify the residual effect of two-tiered intercropping of cotton and groundnut with substitution of 25–50% recommended dose of nitrogen (RDN) of cotton by farmyard manure (FYM) on productivity and soil fertility in a cotton–wheat system at New Delhi during 2006–2008. Wheat following groundnut-intercropped cotton receiving 50% RDN substitution through FYM had significantly 5% greater grain yield than that after sole cotton. Residual soil fertility in terms of organic carbon (C), potassium permanganate (KMnO₄)-N, and dehydrogenase activity (14%) showed an improvement under cotton + groundnut–wheat system with substitution of 50% RDN of cotton by FYM. Apparent N balance as well as actual change in KMnO₄-N at wheat harvest was negative in most of the treatments, with greater loss (–58.1) noticed under pure stand of the cotton–wheat system with 100% RDN of cotton through urea. The study suggested that inclusion of legume and organic manure in transgenic Bt-cotton–wheat system is a sustainable practice for combating escalating prices of N fertilizers with environmental issues and instability of transgenic hybrids in south Asian countries.     

冬种绿肥对水稻土硝化作用的影响

冬闲田种植绿肥是传统的水稻土培肥增产措施,但绿肥-水稻种植系统中,不同绿肥种类对硝化作用的影响规律及调控机制尚不明确.采用盆栽试验,研究了冬种紫云英、油菜、黑麦草对土壤性状及硝化作用的影响,并通过特异性细菌抑制剂(卡那霉素和大观霉素)研究了氨氧化细菌(AOB)和氨氧化古菌(AOA)对硝化作用的相对贡献.结果表明,冬种三...     

长期秸秆覆盖对免耕稻-麦产量、土壤氮组分及微生物群落的影响

为探究长期秸秆覆盖对免耕区作物产量、土壤氮素组分及微生物群落特征的影响,以稻–麦定位免耕试验为研究对象,选取了其中免耕且秸秆移除和免耕且秸秆覆盖2个处理,于试验开展第12年（2018年）小麦收获后,统计分析近五年产量数据,并采集各处理0～5、5～10、10～20、20～30 cm的土壤样品,测定土壤全氮及活性氮组分,利用磷脂脂肪酸（PLFA）方法表征土壤微生物群落。结果表明：（1）秸秆覆盖显著提高了小麦产量（增幅为6.49%）,对水稻产量影响不显著。（2）秸秆覆盖对土壤氮组分的影响略有差异：它显著提高了土壤0～5 cm全氮、硝态氮和铵态氮以及0～10 cm颗粒有机氮、0～5 cm和10～20 cm可溶性有机氮含量,对微生物生物量氮无显著影响;它提高了0～5 cm和10～20 cm可溶性有机氮占全氮的比例,对其他组分占全氮比例无显著影响。（3）秸秆覆盖显著提高了土壤微生物总PLFA和细菌PLFA丰度,对真菌PLFA和放线菌PLFA无影响,降低了土壤真菌/细菌比;微生物生物量氮、土壤全氮、颗粒有机碳/颗粒有机氮比是显著影响土壤微生物群落组成的关键土壤环境因子。（4）无论秸秆覆盖与否,土壤全...     

中国洞庭湖区稻田土壤氮素淋溶损失的系统研究

A two-year lysimeter study was conducted to study the effects of different fertilizers and soils on nitrogen leaching loss in a double rice cropping system by considering three major types of paddy soils from the Dongting Lake area. The results showed that N concentration in the leachate did not differ significantly among the treatments of urea, controlled release N fertilizer and pig manure and that all these fertilizers produced higher total nitrogen (TN) concentrations in the leachate compared to the case where no fertilizer was applied. The TN leaching loss following urea treatment accounted for 2.28%, 0.66%, and 1.50% of the amount of N applied in the alluvial sandy loamy paddy soil (ASL), purple calcareous clayey paddy soil (PCC), and reddish-yellow loamy paddy soil (RYL), respectively. Higher TN loss was found to be correlated with the increased leachate volume in ASL compared with RYL, and the lowest TN loss was observed in the PCC, in which the lowest leachate volume and TN concentration were observed. Organic N and NH4+ -N were the major forms of N depleted through leachate, accounting for 56.8% and 39.7% of TN losses, respectively. Accordingly, soil-specific fertilization regimens are recommended; in particular, the maximum amount of fertilizer should be optimized for sandy soils with a high infiltration rate. To avoid a high N leaching loss from rice fields, organic N fertilizers such as urea or coated urea should primarily be used for surface topdressing or shallow-layer application and not for deep-layer application.     

Corn Monoculture Yield Response to Fertilization and Nitrate Nitrogen Distribution

The objectives of this study were to determine the effects of fertilization system, nitrate (NO₃)– nitrogen (N) distribution along soil profile, and their interaction on corn yield. The study was conducted at the experimental field of Institute of Field and Vegetable Crops in Novi Sad (Serbia) during 2001–2004. Corn monoculture included four fertilization variants: control (Ø), nitrogen–phosphorus–potassium (NPK) mineral fertilizers, mineral fertilizer + corn stalks (NPK + S), and mineral fertilizers + manure (NPK + M). The greatest yield was found in the variant NPK + M (9.25 t ha^?1). Path coefficients showed that greatest direct positive effects on corn yield were exhibited by NO₃-N levels at soil depths of 60–90 cm and 30–60 cm. The path analysis showed that winter precipitation (WP) had a direct negative effect on yield performance. However, the effect of the downward movement of NO₃-N from the topsoil to deeper soil layers of WP on yield was positive.     

Short-Term Irrigation Level Effects on Residual Nitrate in Soil Profile and N Balance from Long-Term Manure and Fertilizer Applications in the Arid Areas of Northwest China

A field experiment was conducted to determine the effects of long-term applications of fertilizers and manure (1982 to 2003) and short-term irrigation level (2002 and 2003) on accumulation of nitrate nitrogen (NO₃-N) in soil at Zhangye Oasis, China. The treatments included manure (M) and no manure (M₀) as main plots; check (Ck), nitrogen (N), nitrogen + phosphorus (NP), and nitrogen + phosphorus + potassium (NPK) as subplots; and two amounts of irrigation (I₁ and I₂) as subsubplots. The application of N alone resulted in large NO₃-N accumulation in soil, accounting for 6% of the applied N, and the lowest crop N recovery. Application of manure resulted in increased NO₃-N in the soil profile compared to treatment with no manure, and the MN treatment resulted in the greatest amount of NO₃-N in soil. Nitrogen applied with P and/or K reduced the amount of NO₃-N in soil in both manure and no-manure treatments compared with N only. The unaccounted N was greatest (60%) in the N-alone treatment and lowest (30%) in the NPK treatment. When manure plus fertilizer were applied together, the unaccounted N ranged from 35%–42%. Based on results from only 2 years, greater amounts of irrigation (I₂) caused greater leaching of NO₃-N in the soil profile compared with I₁, especially in treatments receiving manure. The implications of these findings are that these high amounts of accumulated NO₃-N in surface and subsoil layers can be a potential threat to surface water, underground water, and air quality in the long run. This accumulated N in the soil profile can be used as a source of available N for future crops and should be recycled by using proper crop, soil, fertilizer, and water-management strategies/practices. The findings also suggest the need for further research to make an effective and efficient use of this accumulated NO₃-N in the soil profile in order to save cost of N fertilizer application to future crops.     

不同肥料结构对红壤稻田氮素迁移的影响

不同肥料结构对红壤稻田淹水层、不同深度渗漏水、外排水和土壤剖面中氮素的含量、形态及其动态变化的影响研究结果表明 ,各处理淹水层、外排水和渗漏水中NH4+-N含量明显高于NO3--N。淹水层中N的含量 ,水稻生育前期以单施化肥的高 ,约相当于配施有机肥的 1.18～ 1.20倍 ,而水稻生育后期 ,后者为前者的 1.11～ 1.2 1倍。各处理外排水中N素的输出量均以苗期最高 ,单施化肥明显大于配施有机肥。土壤剖面中NH4+-N向下迁移比碱解N更为明显 ,且配施有机肥的远高于单施化肥的 ,而NO3--N则相反。不同深度渗漏水中NO3--N的比例 ,上层 (30cm)低于下层 (50cm) ,随水逸出的N量各处理渗漏水均小于外排水 ;随水输入的N量远低于随水输出的N量 ,且以单施化肥的N亏损最大。水稻未利用的N量也以单施化肥的最大 ,约为配施有机肥的 1.0 9倍。     

有机无机肥配施对玉米产量及土壤氮磷淋溶的影响

【目的】氮、磷是农作物生长所必需的营养元素,对提高农作物产量和改善产品品质均有重要作用,但由于肥料不合理施用,农田土壤中养分大量盈余,在降雨或灌溉条件下易随水流失,导致水环境质量下降。因此,研究有机无机肥料配施对土壤氮、磷淋溶风险的影响,可为地下水环境质量保护提供依据。【方法】采用田间渗滤池法,对华北地区玉米季氮磷淋溶状况连续5年进行监测,具体施肥处理如下:对照(不施用氮肥,PK)、单施化肥(NPK)、单施有机肥(与NPK处理等氮量,SW)、有机肥无机肥料配施(用猪粪中氮替代50%NPK处理中氮用量,SNP)。采集120 cm处淋溶水,测定氮、磷含量,研究在总氮投入量相同条件下,有机无机肥料配施对华北地区玉米产量及土壤剖面120 cm处氮磷淋溶的影响。【结果】1)有机无机肥料配施(SNP)处理,可以保证玉米较高产量,5年平均产量较单施化肥处理(NPK)提高10.3%。2)有机无机肥料配施可以显著减少总氮(TN)淋溶量,SNP处理较NPK处理减少71.4%;NPK处理淋溶水中NO-3-N浓度显著高于SNP处理,其平均浓度分别为54.93 mg/L、13.47 mg/L。3)在等氮量投入条件下,有机肥的投入带入了大量磷素,单施有机肥(SW)较NPK处理总磷(TP)淋溶量增加了0.6倍,分别为0.056 kg/hm2、0.035 kg/hm2;淋溶水中TP浓度分别为0.09 mg/L、0.066 mg/L。在氮磷养分淋溶损失中,NO-3-N占淋溶水TN的80%以上,可溶性总磷(TDP)占淋溶水TP的70%左右。4)在监测淋溶水中,NPK处理NO-3-N平均浓度已超过我国地下水Ⅲ类水质量标准(GB/T 14848-9),SW处理TP平均浓度0.09 mg/L,也高于水体富营养化TP浓度(0.02 mg/L)的临界值,可对水体造成污染。【结论】在氮磷养分淋溶损失中,NO-3-N占淋溶水TN的80%以上,TDP占淋溶水TP的70%左右。采用猪粪氮替代50%化肥氮素的有机无机肥料配施处理,5年玉米平均产量显著高于单施化肥处理,证明该施肥方法不仅可以确保产量,还可降低氮素淋溶,基本保证淋溶水中NO-3-N浓度低于地下水Ⅲ类水质量标准(GB/T 14848-9)。     

间伐对杉木人工林土壤碳氮及其组分特征的影响

为探究不同间伐强度对杉木人工林土壤碳氮及其组分特征的影响,以福建省三明市官庄国有林场11年生杉木(Cunninghamia lanceolata)人工林为研究对象,采用弱度间伐(LIT)、中度间伐(MIT)、强度间伐(HIT)等3种间伐强度,研究不同间伐强度林分0—10,10—20,20—40,40—60,60—80,80—100 cm土层总有机碳(SOC)、全氮(TN)及易氧化有机碳(ROC)、硝态氮(NO_3~--N)、铵态氮(NH_4~+-N)、微生物量碳(MBC)、微生物量氮(MBN)、微生物熵碳(qMBC)、微生物熵氮(qMBN)的变化特征,以探讨不同间伐强度对杉木人工林土壤碳氮及其组分特征的影响。结果表明:间伐降低了土壤SOC和TN的含量,降低幅度分别为1.4%～36.9%,3.1%～45.7%。间伐增加了土壤MBC、NO_3~--N的含量,而对ROC、NH_4~+-N和MBN的程度在不同土层有差异,qMBC和qMBN随着间伐强度的增加而增大。相关性分析表明,土壤SOC分别与TN、qMBC、ROC、NH_4~+-N、MBC、MBN呈极显著正相关(P0.01);TN与qMBN、ROC、NH_4~+-N、MBC、MBN呈极显著正相关(P0.01)。杉木人工林间伐处理降低了土壤表层SOC和TN含量,增加了土壤SMBC和qMBC、qMBN,同时也增加了土壤表层(0—10 cm)SMBN。抚育间伐导致土壤SOC和TN含量降低主要是由于活性碳、氮含量的增加,提高土壤中有机质分解速率,最终导致土壤SOC和TN含量降低。     

氮肥基追比对小麦产量、土壤水氮分布及利用的影响

为解决区域土壤质地类型针对性氮肥施用问题,在轻壤土和黏壤土上分别设置不施氮肥,氮肥基追比3∶7,4∶6,5∶5,6∶4和7∶3处理,研究小麦产量、水氮利用效率以及土壤含水量、贮水量、NH_4~+-N、NO_3~--N动态变化规律。结果表明:轻壤质土壤氮肥基追比4∶6的处理小麦产量、水分利用效率、氮肥生产效率最高分别为8 265.3 kg/hm~2,27.6 kg/(hm~2·mm),34.4 kg/kg。黏壤质土壤氮肥基追比5∶5的处理小麦产量、水分利用效率、氮肥生产效率最高分别为8 363.2 kg/hm~2,28.3 kg/(hm~2·mm),34.8 kg/kg。小麦不同生育期各土层含水量垂直分布变化较大,轻壤质土壤含水量在9.3%～26.2%,而黏壤质为9.7%～27.6%;小麦全生育期内土壤贮水量呈先升高后降低趋势,黏壤质土壤贮水量高于轻壤质。氮素追施量越多土壤表层NH_4~+-N与NO_3~--N含量越高,且随土层加深土壤NH_4~+-N与NO_3~--N含量降低,受降水影响轻壤质土壤NH_4~+-N与NO_3~--N更易于向土层深处淋溶,成熟期黏壤质各土层的NH_4~+-N和NO_3~--N含量均多于轻壤质。说明黏壤质土壤保水保氮肥能力强于轻壤质,氮肥基追比可以适当增加。     

DAIRY MANURE AND NITROGEN FERTILIZER EFFECTS ON RESIDUAL NITRATE AND PHOSPHATE,AND WHEAT YIELD IN A SANDY CLAY LOAM SOIL

Dairy manure (DM) rates of [0 (DM₀), 30 (DM₃₀₎), 60 (DM₆₀) Mg ha^?1] and three nitrogen (N) rates [0 (N₀), 125 (N₁₂₅), 250 (N₂₅₀) kg ha^?1] were tested in a sandy clay loam, to evaluate their effects on growth and yield of wheat crop (Triticum aestivum L.), residual nitrate nitrogen (NO₃-N) and phosphorus (P) concentrations in the surface soil, and selected soil physical measurements [saturated hydraulic conductivity (Ksat), and bulk density (BD)]. Increasing N and DM rates gave higher wheat yields, increased concentrations of residual NO₃-N and P in the surface soil and improved Ksat and BD. Highest grain yield of 3.8 Mg ha^?1 (70.3% more than the control) was observed in DM₆₀ × N₂₅₀ treatment. Residual accumulation of N-NO₃ and P in the surface soil at high N and/or DM application rates suggests the need to carefully manage N and DM inputs on farm fields to avoid environmental contamination.     

有机肥无机肥配施对稻田氨挥发和水稻产量的影响

在南方红壤区双季稻田进行田间试验,研究等氮、磷、钾量条件下,有机无机肥配施对稻田氨挥发及水稻产量的影响。结果表明,有机无机肥配合施用能显著地降低稻田氨挥发,减少氮素损失,提高氮肥利用率。单施化肥(尿素),其氨挥发损失达37.8%,而单施有机肥和有机无机肥各半配合施用,氨挥发损失分别为0.7％-1.0％和7.2％-18.2％。田间氨挥发持续的时间,早稻约在施肥后20d,晚稻为9-10d。虽然有机无机肥各半配合施用的水稻产量与单施化肥的相近,均比对照提高约70％,但前者的氮损失少,其氮肥利用率为34.9％,高于化肥处理(33.2％)和有机肥处理(28.0%)。有机无机肥配合施用对提高水稻产量和降低氮肥环境负效应的综合效应最佳。     

减氮配施有机物质对麦田土壤性质和小麦产量的影响

探究氮肥减量配施有机物质的情况下对氮素利用状况及土壤肥力和小麦产量的影响,为我国华北平原区小麦生产中提高氮肥利用效率、实现节肥增效提供理论基础。通过田间试验,设置5个处理：不施氮肥(CK)、农民习惯施氮肥(FN)、减氮20%(80%FN)、减氮20%+生物有机肥(80%FN+OM)、减氮20%+生物炭(80%FN+BC),研究小麦生长关键期土壤容重、有机质、NO₃^-—N和土壤微生物多样性的变化,测定小麦产量并计算氮素利用效率。结果表明,土壤容重受施入有机物质影响显著,成熟期0—20,20—40 cm的80%FN+OM、80%FN+BC的土壤容重较80%FN分别下降3.83%～4.58%和2.96%～5.07%。成熟期0—40 cm的土壤有机质均以80%FN+OM最高,较其他施氮肥处理提高2.13%～18.81%。土壤NO₃^-—N受施氮肥影响显著,挑旗期80%FN+OM和80%FN+BC处理的0—40 cm土壤NO₃^-—N较高;灌浆期80%FN+BC处理的0...     

不同有机物料接种蚯蚓对设施菜地土壤 培肥及作物生长的影响

设施蔬菜栽培长期施用过量化肥,往往导致土壤质量退化及作物产量降低等问题。因此,采用生态友好的农业生产方式已成为农业可持续发展的趋势。本研究在野外调控试验的第3年采集作物和土壤,研究了施用不同有机物料条件下,接种赤子爱胜蚓(Eisenia foetida)对设施菜地土壤性质和作物生长的影响。结果表明,在不同有机物料施用下接种赤子爱胜蚓均显著地提高了黄瓜和菠菜的产量,其中在施用腐熟牛粪+食用菌渣条件下接种赤子爱胜蚓效果最显著。此外,在腐熟牛粪和腐熟牛粪+食用菌渣施用条件下,接种赤子爱胜蚓显著地提高土壤的硝态氮(NO3–-N)、团聚体平均重量直径(MWD)、微生物生物量碳(MBC)、微生物生物量氮(MBN)和代谢熵(q CO2),而在施用商品有机肥的处理中,接种赤子爱胜蚓仅显著地提高了土壤NO3–-N和q CO2。本研究促进了对蚯蚓在设施农业生态系统中服务功能的理解,并为设施农业生产提供了理论基础。     

SOIL-CROP NITROGEN RELATIONSHIPS IN MAIZE GROWN ON CALCAREOUS FIELDS

Maize yield is mainly controlled by nitrogen (N) fertilizers. Failure to predict economic optimum N rate causes over-fertilization of N through traditional application methods. Five field trials were conducted to study soil-crop N relationships in maize under field conditions. Optimum level of N application in this study was much lower than conventional N fertilization in calcareous soils of southern Iran, demonstrating over-fertilization of N fertilizers by farmers. Linear regression equations were found between total nitrogen (TN) and chlorophyll meter readings (CMR) (years 2001 and 2002) in both locations demonstrating that chlorophyll meter might be used in predicting TN in maize shoot. Results indicated that soil sampling location influenced on soil NO₃-N concentration at 4–6 leaf stage. In conclusion, N use efficiency can be improved by in-season soil NO₃-N testing. It appears that in Kooshkak and Bajgah experimental stations, soil samples for NO₃-N testing should be collected from shoulder and bottom of furrows, respectively.     

双季稻田添加脲酶抑制剂NBPT氮肥的最高减量潜力研究

【目的】添加脲酶抑制剂（Urease inhibitor, UI）是提高肥料利用率的有效途径,在尿素（Urea,U）中添加1%的脲酶抑制剂NBPT（N-丁基硫代磷酰三胺）是目前研究使用证明效果最可靠的添加比例。针对当前稻田氮肥施用水平过高的问题,本文采用田间小区试验研究了目前脲酶抑制剂添加比例下稻田氮肥的减施潜力以及脲酶抑制剂的节肥增效机理。【方法】本试验在我国长江中下游的双季稻田进行,脲酶抑制剂用量NBPT为尿素用量的1%。尿素用量设五个水平为N 90、 112.5、 135、 157.5 和180 kg/hm2,分别依次记为U1、 U2、 U3、 U4和U5, 7个处理为CK（不施氮肥）、 U1+UI、 U2+UI、 U3+UI、 U4+UI、 U5+UI、 U5（U5为传统施氮量, N 180 kg/hm2为农民习惯施氮量）,三次重复。U1~U5处理施氮量分别是在农民习惯施氮量的基础上降低50%、 37.5%、 25%、 12.5%、 0%。通过取样分析水稻分蘖期和孕穗期各处理对土壤脲酶活性、 硝酸还原酶活性、 土壤铵态氮含量、 硝态氮含量以及微生物量碳、 氮的含量,研究NBPT对水稻两个主要生育期土壤氮素供应的影响,比较各处理的产量以及氮肥利用率来得出氮肥的减施潜力,在此基础上通过逐步回归分析研究以上各指标对产量的影响,探明脲酶抑制剂（NBPT）在双季稻田的增效机理。【结果】 1) 在双季稻田,添加NBPT后,施氮量为N 135 kg/hm2的籽粒产量达到最高。与传统施氮（单施尿素N 180 kg/hm2）处理相比,早、 晚稻可分别增产8.54%和12.87%,氮肥当季利用率分别提高6.78%和9.46%,可节约氮肥25%; 2)与传统施氮相比,添加NBPT显著降低了水稻分蘖期的土壤脲酶活性和铵态氮含量,显著提高了孕穗期的铵态氮含量,而对此时期的脲酶活性无显著影响,NBPT对两个时期的硝酸还原酶活性、 硝态氮含量及微生物量碳、 氮含量均无明显影响,可见基施的NBPT主要是降低尿素水解速率方面效果显著,并且NBPT具有时效性,其主要是在水稻孕穗期之前起作用,在生态上较为安全; 3) 从各项土壤指标与水稻产量相关性的逐步回归分析结果来看,水稻分蘖期与孕穗期稻田土壤中铵态氮含量对水稻产量影响显著,而且孕穗期的影响大于分蘖期,其余指标则对产量无明显影响。【结论】由于脲酶抑制剂NBPT减缓了分蘖期尿素的水解作用,提高了孕穗期土壤中的铵态氮含量,为水稻后期生长提供充足的氮肥,在双季稻减肥方面具有显著的效果。在本试验土壤条件下,尿素中添加1% 的NBPT,可在提高产量的同时,将传统施氮肥量减少25%,是适于稻田应用的脲酶抑制剂。     

Clinoptilolite Zeolite Influence on Nitrogen in a Manure-Amended Sandy Agricultural Soil

Zeolite minerals may improve nitrogen availability to plants in soil and reduce losses to the environment. A study was conducted to determine the influence of clinoptilolite (CL) on nitrogen (N) mineralization from solid dairy manure (224 kg N ha^?1) in a sandy soil. Clinoptilolite was added to soil at six rates (0 to 44.8 Mg CL ha^?1), each sampled during 11 sampling dates over a year. Over time, nitrate (NO₃)-N increased, ammonium (NH₄)-N decreased, but total inorganic N increased. Clinoptilolite did not influence the nitrification rates of initial manure NH₄-N or mineralization of organic N (ON) over time. It is possible that adsorption of manure-derived potassium (K) outcompeted the NH₄-N for CL exchange sites. The ON concentration was constant up to 84 days and then decreased by approximately 18% over the remaining time of the study across all treatments. Clinoptilolite use in this sandy soil did not alter mineralization of N from dairy manure.     

不同氮肥在石灰性潮土中的转化及ATS对尿素氮转化的调控效应

根据氮肥施入土壤后的转化特性进行氮肥的高效调控和管理是提高氮肥利用效率、缓解氮肥污染的重要措施。为探究不同氮肥在石灰性潮土中的转化特性差异及硫代硫酸铵（ammonium thiosulfate，ATS）作为氮肥调控剂对尿素氮转化的影响，该研究采用室内土壤培养（土壤水分含量为田间持水量的60%，温度25 ℃）试验方法，以尿素、硫酸铵、氯化铵和ATS作为供试肥料，比较4种氮肥施入石灰性潮土后的转化特性差异，并以ATS作为氮素调控剂，以单施尿素作为对照，探究尿素配施不同用量ATS对尿素氮转化的影响。结果表明，4种供试氮肥在石灰性潮土中的转化过程明显不同。尿素在石灰性潮土中的水解速率最快，硝化作用强度也最高，硫酸铵其次；氯化铵由于Cl^-的硝化抑制作用，土壤表观硝化率在7～21 d显著低于尿素和硫酸铵（P＜0.05）；ATS施入土壤后，NH₄⁺-N转化为NO₂^--N的速率最高，而NO₂^--N转化为NO₃^--N的速率最低，NH₄⁺-N在土壤中的存留时间最长，出现峰值之后也一直保持最高的含量，表观硝化率最低。将ATS作为氮素调控剂与尿素配合施用，当其用量在60 mg/kg（含S量）以上时，既表现出了明显的抑制尿素水解的作用效果，也表现出了显著的硝化抑制作用（ P <0.05），且随着ATS用量的增加，抑制效应明显增强。这对于减少氮素损失，提高氮肥利用效率具有积极意义。但供试4种氮肥施入土壤后均出现了亚硝酸盐的累积，其中ATS处理的累积量显著高于尿素、硫酸铵和氯化铵（P＜0.05），累积持续时间也最长。ATS作为氮素调控剂调控氮素转化，也出现了类似的结果，且随着ATS用量增加，亚硝酸盐在土壤中存留时间明显延长，含量和峰值明显提高，出现峰值的时间也明显延后。