Assessment,Composition and Possible Source of Organochlorine Pesticides in Surface Soils from rmqi,China

Twenty-eight surface soil samples from 5 functional zones(park, traffic roadside, business/residential area, rural area, and industrial area) of¨Ur¨umqi, China were collected and analyzed for the concentrations of 14 organochlorine pesticides(OCPs), such as 3 isomers of hexachlorocyclohexane(HCHs)(α-HCH, β-HCH, and γ-HCH), 4 dichlorodiphenyltrichloroethanes(DDTs)(p, p′-DDT and its metabolites p, p′-DDD(1,1-dichloro-2,2-bis(p-chlorophenyl)ethane) and p, p′-DDE(1,1-dichloro-2,2-bis(4-chorophenyl)ethylene), and o, p′-DDT), and methoxychlor, aiming to survey the residue levels and compositions of these 14 OCPs, to identify possible sources of the OCPs, and to assess their potential risks to human health and the environment in surface soils of¨Ur¨umqi. The concentrations ranged from non-detected to 30.86 μg kg-1for HCHs(sum of α-, β-, and γ-HCH), from non-detected to 40.03 μg kg-1for DDTs(sum of p, p′-DDT, p, p′-DDD, p, p′-DDE, and o, p′-DDT), and from non-detected to 11.95 μg kg-1for methoxychlor. The total concentrations of the OCPs ranged from 16.40 to 84.86 μg kg-1, with a mean of 41.89 ± 16.25 μg kg-1. According to the measured concentrations and occurrence rates of the 14 OCPs, DDTs, HCHs, and methoxychlor were the most dominant compounds. Among the 5 functional zones, the total concentration of OCPs was in the order of rural area traffic roadside park business/residential area industrial area. The different compositions of DDTs and HCHs indicated that the residues of these compounds in most soil samples originated from historical application, besides slight recent introduction at some sampling sites. The results of Pearson correlation analysis showed that soil total organic carbon played an important role in the residue levels of HCHs, but such relationships were not found with DDTs or other OCPs. The soil quality of¨Ur¨umqi was classified as Class I based on the guidelines of the Chinese Environmental Quality Standard for Soil(GB15618-1995), indicating that the residue levels of OCPs have little risks to the environment and human health in the study area.     

不同施肥方式下土中有机氯农药的残留特征

Soil samples with three fertilization treatments (no fertilizer, corn straw and farm manure) collected from a Lou soil (Eum-orthic Anthrosol classfied using Chinese Soil Taxonomy) in northwestern China were analysed for residual levels and their characteristics of organochlorine pesticides (α-HCH, β-HCH, γ-HCH, δ-HCH, HCB, o,p'-DDT, p,p'-DDT, o,p'-DDE, p,p'-DDE, p,p'-DDD, a-endosulfan, dieldrin and endrin). Organochlorine pesticides (OCPs) were detected in all soil samples except δ-HCH and their total concentrations ranged from 159.31 ± 9.00 to 179.77 ± 2.58 ng g^-1 with an order of HCHs > DDTs > (dieldrin + endrin) > HCB > α-endosulfan. Among all the compounds, γ-HCH had the highest concentration followed by p,p'-DDE. The residual levels of HCH isomers and DDT as well as their metabolites in soil with different fertilization treatments were in the order of γ-HCH > β-HCH ≈ α-HCH > δ-HCH and p,p'-DDE > p,p'-DDT > o,p'-DDT > p,p'-DDD ≈ o,p'-DDE, respectively. DDE/DDT ratios ranged from 1.59 ±0.13 to 3.35 ± 0.16 and endrin/dieldrin ratios from 1.40 ± 0.06 to 9.20 ± 4.05, both indicating no new occurrence of these pesticides in these soils, while α-HCH/γ-HCH ratios of 0.04 indicated a new input of lindane (almost pure γ-HCH) in the past several years. The farm manure treatments showed lower DDT residues than samples without fertilizer. Also addition of corn straw and farm manure increased soil organic matter content and decreased the soil pH which could retard the degradation of DDT in the soil.     

鸭跖草(Commelina communis L.): 发现于中国安徽省的铜的超积累植物

Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707~6159 (4439±2434) mg kg^-1, 369~831 (731±142) mg kg^-1, and 429~587 (547±57) mg kg^-1, respectively, in the roots, stems, and leaves. The soils supporting the growth of the species had a copper concentration ranging from 4620 to 5020 mg kg^-1 and averaging 4835±262 mg kg^-1, suggesting that the species could not only grow on heavily copper-contaminated soils but also accumulate extraordinarily high concentration of copper. Thus, it shows great potential in the phytoremediation of copper-contaminated soils, the restoration of mined land, geochemical prospecting, and the study of environmental pollution changes.     

运用红边参数估算叶片叶绿素含量

Soil samples collected from several sites along an altitudinal transect on the eastern slope of the Tibetan Plateau were analyzed for hexachlorobenzenes(HCHs) and dichlorodiphenyltrichloroethanes(DDTs).The results showed that HCHs and DDTs were found in the soil samples from the remote high altitude areas away from source regions,which confirmed the long-range atmospheric transport phenomenon of these organochlorine pesticides(OCPs) insecticides.The OCP concentrations in the soils had a significant negative correlation with altitude;they showed a trend to increase with decreasing altitude,but the increase was not continuous,being interrupted at some moderate-altitude sites on the transect.The distances from the source region,landforms,soil properties,and physical-chemical properties of OCPs were more important than total organic carbon content to the distribution of OCPs in soils disturbed by human activities.An analysis of the compositions of HCH isomers and DDTs revealed predominantly low ratios of α-HCH to γ-HCH,ranging from 0.06 to 4.79,which suggested current lindane inputs.On the other hand,low p,p-DDT/p,p-DDE and o,p-DDT/p,p-DDT ratios were observed,indicating mainly aged historical DDT residues in the study area.     

基于GIS的亚热带典型地区土壤有机碳空间分布预测

Spatial distribution of organic carbon in soils is difficult to estimate because of inherent spatial variability and insufficient data. A soil-landscape model for a region, based on 151 samples for parent material and topographic factors, was established using a GIS spatial analysis technique and a digital elevation model (DEM) to reveal spatial distribution characteristics of soil organic carbon (SOC). Correlations between organic carbon and topographic factors were analyzed and a regression model was established to predict SOC content. Results for surface soils (0-20 cm) showed that the average SOC content was 12.8 g kg^-1, with the SOC content between 6 and 12 g kg^-1 occupying the largest area and SOC over 24 g kg^-1 the smallest. Also, soils derived from phyllite were the highest in the SOC content and area, while soils developed on purple shale the lowest. Although parent material, elevation, and slope exposure were all significant topographic variables (P < 0.01), slope exposure had the highest correlation to SOC content (r = 0.66). Using a multiple regression model (R² = 0.611) and DEM (with a 30 m × 30 m grid), spatial distribution of SOC could be forecasted.     

中国某些土壤中的水溶性稀土元素

Water-soluble rare earth elements (WSREEs) of four typical soil profiles in China were determined by using a high-resolution inductively coupled plasma mass spectrometer. Results showed that the contents of WSREEs decreased from upper layer to lower layer of soils in the southern part of China with a high rainfall and low pH but increased for soils in the northern part of China with a low rainfall and relatively higher pH. Contents of WSREEs in soils were lower than 100 μg kg^-1 in most cases, and varied greatly with both different soils and different layers of the same profile. The highest content was 2816.3 μg kg^-1 but the lowest was 17.6 μg kg^-1 only. The content of individual rare earth lement (REE) in the soil solution also varied greatly with the highest one ranging from 8.4 to 1373 μg kg^-1 for Ce and the lowest one from 0.05 to 4.48 μg kg^-1 for Lu. The sum of WSREEs in the first soil layers ranged from 121.5 to 345.6 μg kg^-1. Great variations existed among ratios of REEs extracted with water to the total REEs of soils, ranging from 0.02×10^-3 to 13.2×10^-3. But as the upper layer was considered, the ratio showed only a small difference, ranging from 0.79×10^-3 to 1.69×10^-3.     

漳州土壤中有机氯农药残留研究

A soil survey was conducted in Zhangzhou City,an important agricultural region in south of the Fujian Province,China.93 surface soil samples were collected in the paddy fields,vegetable lands,orchards and tea plantations from Zhangzhou City.An additional soil profile was sampled in a paddy field as previous research had indicated high concentrations of organochlorine pesticides(OCPs) in the paddy fields.Dichlorodiphenyltrichloroethanes(DDTs) ranged from 0.64-78.07 ng g 1 dry weight and hexachlorocyclohexanes(HCHs) ranged from 0.72-30.16 ng g 1 dry weight in the surface soil of the whole study region.Ratios of α-HCH/γ-HCH < 4 and o,p-DDT/p,p-DDT > 1 in all soil samples suggested that lindane and dicofol were widely applied in this region in the past.Concentrations of HCHs and DDTs in soils from the four land use types followed the orders:paddy fields > vegetable lands > tea plantations > orchards and tea plantations > orchards > paddy fields > vegetable lands,respectively.Analyses of the data showed no correlation(r < 0.1) between elevation and OCPs contents in paddy fields,orchards and vegetable lands,indicated no significantly different features in distribution of HCHs and DDTs in the soils from low lying plains and mountains and the unsystematic usage of OCPs,and highlighted the fragmented nature of agricultural production in Zhangzhou,as well as the reemission of OCPs from the soils,where high OCPs concentrations were found,in Longhai of Zhangzhou.In addition,no obvious relationship between the OCPs and total organic carbon(TOC)(r < 0.3) was observed in the soil profile.The mean contribution of dicofol in total DDTs was 66% in the whole Zhangzhou region.The approximate burdens of HCHs and DDTs in the surface layer of 0-20 cm were 0.44 and 1.55 t,respectively.The storage of both HCHs and DDTs in soil surface layer(0-20 cm) accounts for 40% burden of the soil layer of 0-50 cm(1.10 t HCHs and 3.87 t DDTs),in which the highest concentrations of OCPs were observed in soil profile.     

不同液土比和温度条件下塿土铵离子吸附

Effects of NH₄⁺ concentration, solution/soil ratio and temperature on NH₄⁺ adsorption were studied in a Eum-Orthic Anthrosol. The slopes of the soil NH₄⁺ adsorption isotherms and the fitted n, the coefficient for the adsorption intensity, and k, the coefficient related to adsorption capacity, of the Freundlich equation increased with increasing solution/soil ratio (SSR) and with decreasing temperature (T). For the range of experimental conditions, the value of ∂q/∂c, the rate of change of the amount of NH₄⁺ adsorbed in the soil solid phase (q) with respect to the equilibrium concentration of NH₄⁺ in soil solution (c), was 0.840, indicating that q increased with increasing c. From 2 to 45 ℃, ∂q/∂SSR, the rate of change of q with respect to SSR, decreased from 2.598 to 1.996, showing that q increased with increasing SSR, while its increasing rate decreased with temperature. From SSR 1:1 to 20:1, ∂q/∂T, the rate of change of q with respect to T, decreased from -0.095 to -0.361, indicating that q decreased with increasing temperature, and at the same time the negative effect of temperature became larger as SSR increased. Thus under the experimental conditions the order of importance in determining the amount of NH₄⁺ adsorbed in the soil solid phase was ∂q/∂SSR > ∂q/∂c > |∂q/∂T|, indicating that the greatest effect on the amount of NH₄⁺ adsorbed was with the solution/soil ratio; the equilibrium concentration of NH₄⁺ had a lesser effect; and temperature had the least effect.     

农作物的红边特征与农学参数的关系

The hyperspectral reflectance of the canopy and the leaves on the main stem for six varieties, two each of rice, corn, and cotton crops, were measured at different growth stages with an ASD FieldSpec Pro FR^TM to analyze red edge characteristics for leaf area indices (LAI), aboveground biomass, as well as the chlorophyll, carotenoid, and nitrogen content, emphasizing comparative differences on the red edge parameters. The results showed a ‘double peak' phenomenon for the red edge of the canopy spectra but not for the leaves. There were ‘increase' and ‘decrease' change rules for the red edge position, λ_r, the red edge slope, Dλ_r, and the red edge area, S_r, of the canopy spectra for all 3 crops with a ‘blue shift' for λ_r of the leaf spectra for all 3 crops as the development stages progressed. For rice, corn, and cotton the LAI and fresh leaf mass had highly significant correlations (P < 0.01) so the red edge parameters λ_r, Dλ_r, and S_r of their canopy spectra. Additionally, for all crops the chlorophyll-a, chlorophyll-b, total chlorophyll, and carotenoid content of the leaves all had highly significant (P < 0.01) correlations to their λ_r. For rice, the nitrogen content of the leaves in g kg^-1 and phytomass for a unit area of land in g m^-2 also had a highly significant (P < 0.01) correlation to λ_r, Dλ_r, and S_r of the canopy spectra.     

华中地区旱地氮素矿化的能力

Nitrogen mineralization potentials of 15 soil samples were studied by the methods of soil aerobic incubation, and the correlation between the potentials and the amounts of nitrogen taken up by rye grass (Lolium multiflorum Lam.) in pot culture was calculated. The soils were collected from Hubei Province in Central China. Soil nitrogen mineralization potentials (N_O) were calculated and optimized by a quick-BASIC program. N_O ranged from 60 mg kg^-1 to 340 mg kg^-1, which accounted for 9.1% to 34.6% of the total nitrogen content. Among the examined soils, yellow-brown soil collected from Wuhan had the largest N_O and brown-red soil from Xianning had the smallest one. The mineralization rate constants (k) ranged from 0.00556 d^-1 to 0.01280 d^-1, in average 0.00882 d^-1. Chao soil from Wuhan had the greatest k while yellow-cinnamon soil from Zhaoyang had the smallest one. There were apparent differences between mineralization parameters (N_O and k) optimized and non-optimized ones. Optimized N_O had a better correlation than non-optimized N_O with the amount of nitrogen accumulated in the aerial parts of rye grass. N_O, N_O × k and N_t(accumulated mineralized nitrogen within time t) could be used as indexes of soil nitrogen supply. Among them N_t was the best, which was significantly correlated with the amounts of nitrogen accumulated in the aerial parts of rye grass harvested at three different times.     

Irrigation Module and Sowing Date Affect Seed Cotton Yield,Quality, Productivity Indices,and Economics of Cotton in North-western India

ABSTRACT

Delayed sowing and imprecise application of irrigation water to cotton has been the major hurdle in sustaining cotton yield in north-western India. Therefore, studies were initiated to ascertain the impact of heavy or normal level of presowing irrigation (PSI), scheduling time of first postsowing irrigation (POSI) under two sowing dates (SDs) on cotton arranged in a split block design replicated thrice. PSI_h (100 mm) recorded 23.2% higher seed cotton yield over PSI_n (70 mm) owing to better yield attributes and higher stand. First POSI at 4 weeks after sowing (WAS) resulted the highest yield (3072 kg ha^?1), while one at 6 WAS (POSI₆) recorded least due to poor population and reduced yield attributes. Water productivity under POSI₆ was lesser by 29.1%, 25.2%, and 16.2% as compared to POSI₃, POSI₄, and POSI₅, respectively. April sown crop out yielded the May sown cotton crop by 939 kg ha^?1 due to better yield attributes. Nitrogen factor productivity (NFP) among SD remained higher by 23.3% for April as compared to May sowing. PSI_h exhibited better NFP over PSI_n. POSI₆ recorded least NFP by 24.4%, 28.5%, and 16.3% as compared to POSI₃, POSI₄, and POSI₅, respectively and was indicative of relatively poor utilization of N under delayed schedules. Therefore, planting in April after heavy PSI and scheduling first POSI at 4 WAS is best strategy for sustaining cotton yield and maximizing farmer profitability.     

河北省土壤有机碳密度的估算与不确定性来源研究

In order to improve the precision of soil organic carbon (SOC) estimates, the sources of uncertainty in soil organic carbon density (SOCD) estimates and SOC stocks were examined using 363 soil profiles in Hebei Province, China, with three methods: the soil profile statistics (SPS), GIS-based soil type (GST), and kriging interpolation (KI). The GST method, utilizing both pedological professional knowledge and GIS technology, was considered the most accurate method of the three estimations, with SOCD estimates for SPS 10% lower and KI 10% higher. The SOCD range for GST was 84% wider than KI as KI smoothing effect narrowed the SOCD range. Nevertheless, the coefficient of variation for SOCD with KI (41.7%) was less than GST and SPS. Comparing SOCD's lower estimates for SPS versus GST, the major sources of uncertainty were the conflicting area of proportional relations. Meanwhile, the fewer number of soil profiles and the necessity of using the smoothing effect with KI were its sources of uncertainty. Moreover, for local detailed variations of SOCD, GST was more advantageous in reflecting the distribution pattern than KI.     

近红外光谱法快速测定土壤碱解氮、速效磷和速效钾含量

运用偏最小二乘法(PLS)和人工神经网络(ANN)方法分别建立了0.9 mm筛分风干黑土土壤碱解氮、速效磷和速效钾含量预测的近红外光谱(NIRS)分析模型。使用偏最小二乘算法建立的碱解氮、速效磷和速效钾校正模型的决定系数R²分别为0.9520、0.8714和0.7300，平均相对误差分别为3.42%、13.40%和7.40%。人工神经网络方法建立的碱解氮、速效磷和速效钾校正模型的决定系数分别为0.9563、0.9493和0.9522，相对误差分别为2.67%、6.48%和2.27%，测试集仿真的相对误差分别为5.44%、16.65%和7.87%。结果表明，人工神经网络方法所建立的校正模型均优于偏最小二乘法所建模型；用近红外光谱分析法预测土壤碱解氮含量是可行的，而速效磷、速效钾模型的测试集样品仿真的相对误差较大，其预测可行性还需做进一步研究。     

Changes in soil hydraulic conductivity, runoff, and soil loss due to irrigation with different types of saline–sodic water

Irrigation with saline–sodic water causes sodic conditions in the soil which reduces soil productivity. We evaluated the changes in a number of important indices related to soil structural stability when treated wastewater (TWW), albeit with higher loads of organic matter and suspended solids, was used instead of more saline–sodic irrigation water, under different degrees of aggregate slaking. We studied soil saturated hydraulic conductivity (HC) using disturbed samples packed in columns, and soil infiltration rate, runoff and erosion under simulated rainfall. Aggregate slaking was manipulated by wetting the samples prior to all tests at either a slow (1–2 mm h^− 1) or a fast (50 mm h^− 1) rate. Samples of a calcareous silty clay (Typic Calciorthids) from the Bet She'an Valley, Israel, were taken from plots irrigated for three years with either TWW, saline–sodic Jordan River water (JRW), or moderately saline–sodic spring water (SPW), and also from a non-cultivated area (control). With little or no aggregate slaking (use of slow wetting), higher HC values and lower amounts of total runoff and soil loss were measured compared to when more severe aggregate slaking was induced (use of fast wetting). The HC values for the TWW treatment were similar to, or lower than, those for the control and significantly higher than those for the JRW treatment. For the runoff and soil loss data, differences among the water quality treatments were, generally, more pronounced when aggregate slaking was substantially reduced, and were related to soil sodicity. Under the latter condition, runoff and soil loss from the TWW treatment were comparable with those from the control and significantly lower than those from the JRW treatment. Our results suggested that replacing saline–sodic irrigation water with TWW could have favorable effects on soil structural stability, especially under conditions where aggregate slaking can be reduced (e.g., in regions with low to moderate rain intensities; and/or use of low intensity irrigation systems).     

氮磷钾不同配比对饲用稻威优198产量及糙米蛋白含量的影响

采用田间小区试验研究“推荐配比”(N:P2O5:K2O比率为190:90:100 kg hm-2)、“高氮量配比”(N:P2O5:K2O比率为210:90:100 kg hm-2)、“低氮量配比”(N:P2O5:K2O比率为170:90:100 kg hm-2)以及“常规配比”(N:P2O5:K2O比率为216:112.5:202.5 kg hm-2)4种氮、磷、钾配比施肥对饲用稻威优198蔗糖合成酶(SUS)、腺苷二磷酸焦磷酸化酶(AGPase)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)以及产量和糙米蛋白质的影响。结果表明:“推荐配比”能提高不同生育时期水稻功能叶(旗叶)和粒籽中碳、氮代谢关键酶的活性,这些关键酶活性的变化显著影响水稻产量和糙米全氮以及蛋白氮的含量。统计(P0.05)结果证实“推荐配比”能提高水稻产量达到8200 kg hm-2,与“常规配比”相比产量提高了24.81%;“推荐配比”糙米全氮和蛋白氮含量分别达到22.70 g kg-1和21.98 g kg-1,与“常规配比”相比差异显著,并且其全氮和蛋白氮含量分别提高17.01%,18.38%。     

Pb,Zn对超富集植物(小鳞苔草)抗氧化酶活性的影响

通过人工气候箱盆栽实验,研究Pb,Zn及其复合胁迫对小鳞苔草(Carex gentilisFranch.)根和叶中SOD,POD及CAT活性的影响。结果表明:叶片SOD活性随Pb和Zn浓度增加均呈缓慢上升趋势,根部SOD则表现为有升有降的过程,Pb Zn复合处理可提高根部和叶片SOD活性,最高分别为201.8 U/gFW和233.1U/gFW。根部POD活性随Pb,Zn浓度增加均出现不同程度提高,但叶片POD活性呈有升有降的过程;根部和叶片POD在Pb Zn复合处理中活性增幅明显,最高分别为对照的7.47倍和1.49倍。根部和叶片中CAT在Pb,Zn及其复合处理中活性均受到不同程度的抑制。方差分析显示,Pb对根部3种酶活性影响显著;Zn对叶片POD活性影响显著;Pb Zn互作对植株体内POD和根部SOD影响极显著。经综合分析,小鳞苔草在Pb,Zn及其复合胁迫条件下,SOD和POD起主要作用,并具有耐Pb高浓度胁迫的抗氧化酶活性调控能力,可望在中国西部铅锌复合污染区植物修复中提供一种新资源。     

Emission of dimethyl sulfide,carbonyl sulfide,and carbon bisulfide from paddy fields

Abstract

Emission rates of dimethyl sulficle (DMS), carbonyl sulfide (COS), and carbon disulfide (CS₂) to the atmosphere from paddy fields at Ryugasaki, Ibaraki Prefecture, Japan were measured by using the closed chamber method. DMS was emitted mainly through the rice plant and its emission rate was much higher than those of COS and CS₂. During a cropping period COS was slightly absorbed by the rice plant. Significant diurnal and seasonal variations of DMS iuxes were observed. The highest DMS iux was observed immediately after the heading day of rice plant. The annual DMS emission rate was in the order of mineral plot > straw plot > no-N plot, ranging from 4.5 to 6.9 mg S m^-2 yr^-1. The annual COS emission rate was in the order of straw plot > no-N plot > mineral plot, ranging from -0.2 to 1.8 mg S m^-2 yr^-1. The annual CS₂ emission rate was slightly higher in the straw plot, ranging from 0.9 to 2.0 mg S m^-2 yr^-1.     

Modulation in growth,development, and yield of Camelina sativa by nitrogen application under water stress conditions

Camelina sativa has rejuvenated as a successful oilseed crop in the recent years. It is a low-input-requiring crop with an unusual fatty acid composition. A pot experiment was conducted in rain-out shelter to investigate the effect of different nitrogen levels (N₀ = 0, N₁ = 50, N₂ = 100, and N₃ = 150 kg ha^?1) on the growth and yield of two C. sativa genotypes (Australian and Canadian) under normal [100% field capacity (FC)] and water stressed (60% FC) conditions. The experiment was laid out in a completely randomized design with factorial arrangement having three replicates in the Department of Crop Physiology, University of Agriculture, Faisalabad. The results indicated that nitrogen doses and water stress levels significantly affected the growth and yield of C. sativa. Maximum values for growth indices like leaf area index (LAI), crop growth rate (CGR), leaf area duration (LAD), net assimilation rate (NAR), and yield attributes were observed under N₂ treatment (100 kg ha^?1) followed by the treatment in which nitrogen was applied at the rate of 50 kg ha^?1. However, growth and yield components significantly reduced under water stress conditions (60% FC). Of both the genotypes, Australian Camelina performed better as compared to Canadian Camelina under both non-stress and drought stress conditions.     

黄土区不同类型土壤微生物量碳、氮和可溶性有机碳、氮的含量及其关系

研究了黄土高原南部地区不同土壤类型及不同利用方式下土壤微生物摄碳、氮和可溶性有机碳、氮的含量。结果表明：不同土地利用方式下，土壤微生物量碳、氮和可溶性有机碳、氮含量均为林地〉农田，其中林地枯枝落叶层〉林地O～20cm土层。农田土壤微生物量碳、氮的含量均为红油土〉黑垆土〉淋溶褐土；农田土壤中可溶性有机碳含量为淋溶褐土〉红油土〉黑垆土，而可溶性有机氮含量则为黑垆土〉红油土〉淋溶褐土。方差分析表明，不同土壤类型土壤微生物量氮含量之间的差异达显著水平，而不同土壤类型间土壤微生物量碳、可溶性有机碳、氯含量之间的差异未达显著水平。土壤微生物量碳、氮占土壤有机碳和全氮的比例明显高于可溶性有机碳、氮占土壤有机碳和全氮的比例。相关分析发现，土壤微生物量碳与可溶性有机碳之间以及土壤微生物量氮与可溶性有机氮之间的相关性达显著或极显著水平，说明土壤微生物量碳、氮和土壤可溶性有机碳、氮之间有密切联系。     

秸秆直接还田与炭化还田对潮土硝化微生物的影响

为比较秸秆直接还田和炭化还田对黄淮海平原小麦/玉米轮作体系典型潮土硝化作用及硝化微生物群落的影响,设置4个处理:全量小麦秸秆还田(S)、全量秸秆炭化还田(B)、半量秸秆半量生物质炭还田(SB)和不进行秸秆或生物质炭还田的对照(CK),连续进行3 a田间试验。对小麦、玉米两个生长季土壤理化性质进行分析,用末端限制性片段长度多态性(Terminal-restriction length polymorphism,T-RFLP)技术和克隆文库技术对氨氧化古菌(Ammonia-oxidizing archaea,AOA)和氨氧化细菌(Ammonia-oxidizing bacteria,AOB)群落结构和多样性进行分析。结果表明,在小麦季,与S处理相比,B处理显著降低了土壤容重,提高了土壤pH、有机碳(SOC)和速效钾(AK)含量(P0.05),但并未显著影响土壤水分、铵态氮(NH_4~+-N)和硝态氮(NO_3~--N)含量;B和SB处理的硝化潜势(Potential nitrification rate,PNR)分别为0.58、0.49μg·h~(-1)·g~(-1)(以NO_2~-计,下同),显著高于CK,与S处理(0.40μg·h~(-1)·g~(-1))差异不显著。玉米季,B处理显著提高了土壤水分、SOC和AK(P0.05),各处理玉米季的PNR整体低于小麦季,B处理最高(0.27μg·h~(-1)·g~(-1)),显著高于CK和S处理(P0.05)。小麦季PNR分别与AK、NH_4~+浓度和土壤容重显著相关(P0.05),与AOA和AOB群落组成均无显著关系;玉米季PNR仅与理化因子SOC显著相关,但该季节PNR与AOB群落结构显著相关。冗余分析(RDA)表明,土壤SOC、容重、pH和AK是显著影响硝化微生物群落结构的主要因子,对AOA和AOB群落结构总变异的解释量分别为76.4%和75.5%。系统发育树分析表明,AOA大部分属于土壤古菌Group1.1b,AOB多属于亚硝化螺菌Nitrosospira簇3。综上,与秸秆直接还田相比,炭化还田提高土壤硝化活性,改善部分土壤理化性质,引起土壤硝化微生物群落结构变化。