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1.
In 11 rain‐fed arable soils of the Potohar plateau, Pakistan, the amounts of microbial‐biomass C (Cmic), biomass N (Nmic), and biomass P (Pmic) were analyzed in relation to the element‐specific total storage compartment, i.e., soil Corg, Nt, and Pt. The effects of climatic conditions and soil physico‐chemical properties on these relationships were highlighted with special respect to crop yield levels. Average contents of soil Corg, Nt, and Pt were 3.9, 0.32, and 0.61 mg (g soil)–1, respectively. Less than 1% of Pt was extractable with 0.5 M NaHCO3. Mean contents of Cmic, Nmic, and Pmic were 118.4, 12.0, and 3.9 µg (g soil)–1. Values of Cmic, Nmic, Pmic, soil Corg, and Nt were all highly significantly interrelated. The mean crop yield level was closely connected with all soil organic matter– and microbial biomass–related properties, but showed also some influence by the amount of precipitation from September to June. Also the fraction of NaHCO3‐extractable P was closely related to soil organic matter, soil microbial biomass, and crop yield level. This reveals the overwhelming importance of biological processes for P turnover in alkaline soils. 相似文献
2.
Field estimation of the microbial biomass of soils derived from natural and anthropogenic parent materials Whereas the estimation and evaluation of physical and chemical soil properties is possible with the use of widely accepted methods, there is still no procedure with respect to microbiological parameters. Therefore, our objective was to develop a field procedure for estimating the integral parameter ”︁microbial biomass”. For this purpose, microbial biomass data (Cmic in dry matter) of soils developed in natural parent materials from 116 sites in Germany and abroad were analyzed. Additionally, Cmic in 33 German soils developed in anthropogenic parent materials was determined. In the soils under consideration, Cmic varied between 100 and 4000 kg ha—1 in the upper 30 cm. For soils of natural substrates statistical relations between Cmic and the humus and clay content as well as pH were derived. From these parameters which are combined as the diagnostic characteristics of humus a simple procedure for estimating Cmic of arable soils was developed. For soils developed in anthropogenic parent materials, however, an estimation procedure was developed that uses the nature of the parent material and the degree of soil development. To evaluate the estimated amount of Cmic, a frame consisting of 6 classes is proposed: < 200 kg ha—1 (= very low), 200—400 kg ha—1 (= low), 400—800 kg ha—1 (= moderate), 800—1600 kg ha—1 (= medium), 1600—3200 kg ha—1 (= high), and > 3200 kg ha—1 (= very high). 相似文献
3.
蒙古高原草原土壤微生物量碳氮特征 总被引:48,自引:0,他引:48
沿着水分梯度采集了蒙古高原不同草原类型表层土壤样品 1 44个 ,分析了土壤微生物量C、N含量及其与年平均温度和降雨量的关系。结果表明 :蒙古高原草原土壤微生物量C、N与土壤有机C、全N、降雨量、温度均表现出了很好的相关性。微生物量C变化在5 1 7~ 797mgkg- 1之间 ,微生物量N变化在 1 1 0~ 1 1 8 6mgkg- 1之间。微生物量C∶N比变化在 5~ 9之间。土壤微生物量碳 (Cmic)占土壤有机碳 (Corg)的比例 (Cmic Corg)变化在 1 1 5 %~ 4 1 %之间 ,Cmic Corg与土壤有机C、全N、降雨量均成显著的负相关。土壤呼吸表现为草甸草原土壤 >典型草原 >荒漠草原 ,土壤呼吸与降雨量显著正相关 ,与温度显著负相关。呼吸熵 (QCO2 )与降雨量成二次抛物线关系。放牧对微生物量的影响与不同草原类型和放牧率有关。 相似文献
4.
Jürgen K. Friedel Otto Ehrmann Michael Pfeffer Michael Stemmer Tobias Vollmer Michael Sommer 《植物养料与土壤学杂志》2006,169(2):175-184
Microbial biomass, respiratory activity, and in‐situ substrate decomposition were studied in soils from humid temperate forest ecosystems in SW Germany. The sites cover a wide range of abiotic soil and climatic properties. Microbial biomass and respiration were related to both soil dry mass in individual horizons and to the soil volume in the top 25 cm. Soil microbial properties covered the following ranges: soil microbial biomass: 20 µg C g–1–8.3 mg C g–1 and 14–249 g C m–2, respectively; microbial C–to–total organic C ratio: 0.1%–3.6%; soil respiration: 109–963 mg CO2‐C m–2 h–1; metabolic quotient (qCO2): 1.4–14.7 mg C (g Cmic)–1 h–1; daily in‐situ substrate decomposition rate: 0.17%–2.3%. The main abiotic properties affecting concentrations of microbial biomass differed between forest‐floor/organic horizons and mineral horizons. Whereas microbial biomass decreased with increasing soil moisture and altitude in the forest‐floor/organic horizons, it increased with increasing Ntot content and pH value in the mineral horizons. Quantities of microbial biomass in forest soils appear to be mainly controlled by the quality of the soil organic matter (SOM), i.e., by its C : N ratio, the quantity of Ntot, the soil pH, and also showed an optimum relationship with increasing soil moisture conditions. The ratio of Cmic to Corg was a good indicator of SOM quality. The quality of the SOM (C : N ratio) and soil pH appear to be crucial for the incorporation of C into microbial tissue. The data and functional relations between microbial and abiotic variables from this study provide the basis for a valuation scheme for the function of soils to serve as a habitat for microorganisms. 相似文献
5.
Arylsulfatase activity of microbial biomass in soils as affected by cropping systems 总被引:5,自引:0,他引:5
The impacts of crop rotations and N fertilization on different pools of arylsulfatase activity (total, intracellular, and
extracellular) were studied in soils of two long-term field experiments in Iowa to assess the contibution of the microbial
biomass to the activity of this enzyme. Surface-soil samples were taken in 1996 and 1997 in corn, soybeans, oats, or meadow
(alfalfa) plots that received 0 or 180 kg N ha–1 before corn, and an annual application of 20 kg P ha–1 and 56 kg K ha–1. The arylsulfatase activity in the soils was assayed at optimal pH (acetate buffer, pH 5.8) before and after chloroform fumigation;
microbial biomass C (Cmic) and N (Nmic) were determined by chloroform-fumigation methods. All pools of arylsulfatase activity in soils were significantly affected
by crop rotation and plant cover at sampling time, but not by N fertilization. Generally, the highest total, intracellular,
and extracellular arylsulfatase activities were obtained in soils under cereal-meadow rotations, taken under oats or meadow,
and the lowest under continuous cropping systems.Total, intracellular, and extracellular arylsulfatase activities were significantly
correlated with Cmic (r>0.41, P<0.01) and Nmic (r>0.38, P<0.01) in soils. The averages of specific activity values, i.e., of arylsulfatase activity of the microbial biomass, expressed
per milligram Cmic, ranged from 315 to 407 μg p-nitrophenol h–1. The total arylsulfatase activity was significantly correlated with the intracellular activity, with r values >0.79 (P<0.001). In general, about 45% of the total arylsulfatase activity was extracellular, and 55% was associated with the microbial
biomass in soils, indicating the importance of the microflora as an enzyme source in soils.
Received: 23 April 1998 相似文献
6.
《Communications in Soil Science and Plant Analysis》2012,43(20):2679-2690
Soil, crop, and fertilizer management practices may affect quality of organic carbon (C) and nitrogen (N) in soil. A long-term field experiment (growing barley, wheat, or canola)was conducted on a Black Chernozem (Albic Argicryoll) loam at Ellerslie, Alberta, Canada, to determine the influence of 19 years (1980 to 1998) of tillage [zero tillage (ZT) and conventional tillage (CT)], straw management [straw removed (SRem) and straw retained (SRet)], and N fertilizer rate (0, 50, and 100 kg N ha?1 in SRet and 0 kg N ha?1 in SRem plots) on macro-organic matter C (MOM-C) and N (MOM-N), microbial biomass C (MB-C), and mineralizable C (Cmin) and N (Nmin) in the 0- to 7.5-cm and 7.5- to 15-cm soil layers. Treatments with N fertilizer and SRet generally had a greater mass of MOM-C (by 201 kg C ha?1 with 100 kg N ha?1 rate and by 254 kg C ha?1 with SRet), MOM-N (by 12.4 kg N ha?1 with 100 kg N ha?1 rate and by 8.0 kg N ha?1 with SRet), Cmin(by 146 kg C ha?1 with 100 kg N ha?1 rate and by 44 kg C ha?1 with SRet), and Nmin(by 7.9 kg N ha?1 with 100 kg N ha?1 rate and by 9.0 kg N ha?1 with SRet)in soil than the corresponding zero-N and SRem treatments. Tillage, straw, and N fertilizer had no consistent effect on MB-C in soil. Correlations between these dynamic soil organic C or N fractions were strong and significant in most cases, except for MB-C, which had no significant correlation with MOM-C and MOM-N. Linear regressions between crop residue C input and mass of MOM-C, MOM-N, Cmin, and Nmin in soil were significant, but it was not significant for MB-C. The effects of management practices on dynamic soil organic C and N fractions were more pronounced in the 0- to 7.5-cm surface soil layer than in the 7.5- to 15-cm subsoil layer. In conclusion, the findings suggest that application of N fertilizer and retention of straw would improve soil quality by increasing macro-organic matter and N-supplying power of soil. 相似文献
7.
To evaluate the validity of different indices in estimating soil readily mineralizable N, soil microbial biomass (Nmic), soil active N (SAN), soluble organic N (SON), net N mineralization rate (NNR) and gross N mineralization rate (GNR) in mineral soils (0-10 cm) from six forest stands located in central Germany were determined and compared with two sampling times: April and November. Additionally, soil density fractionation was conducted for incubated soils (with addition of ^15NH4-N and glucose, 40 days) to observe the sink of added ^15N in different soil fractions. The study showed that Nmic and NNR in most stands differed significantly (P 〈 0.05) between the two sampling times, but not GNR, SAN and SON. In November, no close relationships were found between GNR and other N indices, or between Nrnic, SON, and SAN and forest type. However, in April, GNR was significantly correlated (P 〈 0.05) with Nmic, SAN, and NNR along with Nmlc under beech being significantly higher (P 〈 0.05) than under conifers. Furthermore, density fractionation revealed that the light fraction (LF, 0.063-2 mm, 〉 1.7 gcm^-3) was not correlated with the other N indices. In contrast, results from the incubation study proved that more 15N was incorporated into the heavy fraction (HF 〈 0.063 ram, 〉 1.7 g cm^-3) than into LF, indicaing that more labile N existed in HF than in LF. These findings suggested that attention should be paid to the differences existing in N status between agricultural and forest soils. 相似文献
8.
Tree species differ in their effect on soil development and nutrient cycling. Conversion of beech coppice to pine plantations can alter soil physical and chemical properties, which in turn may have significant impacts on soil microbial biomass C and N (Cmic, Nmic). The major objective of this study was to evaluate soil quality changes associated with the forest conversion in humid NW Turkey. Results from this study showed that levels of soil organic carbon (Corg), total nitrogen (Nt), moisture, Cmic and Nmic under beech coppice were consistently higher but levels of pH, CaCO3 and EC were lower compared to pine plantation. Differences between the forest stands in Cmic and Nmic were mainly related to the size of the Corg stores in soil and to tree species. In addition, high level of CaCO3 is likely to reduce pools of soil organic C and possibly even microbial biomass C and N in pine forests. The average Cmic:Nmic ratios were higher in soils under beech coppice than pine plantation, while Cmic:Corg and Nmic:Nt percentages were similar in both forest types. These results revealed the differences in microbial community structure associated with different tree species and the complex interrelationships between microbial biomass, soil characteristics, litter quantity and quality. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
9.
The following parameters were measured on seven field plots at 3 sites which had been under organic farming for different periods of time: mineral nitrogen (N min) contents, in situ net nitrogen mineralization (N net), soil microbial biomass carbon (C mic), and nitrogen (N mic) contents, and extractable organic N contents. The measurements were conducted every three weeks from spring 1995/1996 to autumn 1997. The objective was to test whether, under organic farming: 1) temporal fluctuations of Nmic contents over the course of the year are indicative for a source‐and‐sink function for plant‐available N of the soil microbial biomass, and 2) temporal variations in Nmic content can be related with in situ Nnet or plant N uptake. Nmin contents gradually increased after ploughing in autumn until late winter. During intensive plant growth in spring, values rapidly declined. In situ Nnet fluctuated only moderately and reached high values during intensive plant growth (May—July) as well as after soil cultivation in autumn. The Cmic and Nmic contents generally were low in winter, increased in spring and reached maxima in late spring or summer. In spring, the increase in Cmic contents preceded the increase in Nmic contents, resulting in elevated Cmic:Nmic ratios until shooting of winter wheat. This corresponds to an uptake of available soil nitrogen by the plants at the expense of soil micro‐organisms. The subsequent increase in Nmic contents, coinciding with high plant N uptake rates, indicates an enhanced, plant‐induced N mobilization at that time. Possible mobilization mechanisms are discussed. Soil microbial biomass exerted a source‐and‐sink function for extractable organic N on some of the field plots. Estimates of in situ Nnet measurements were neither correlated significantly with soil microbial biomass N, Nmic flux, Nmic turnover, nor with plant N uptake. Lower Nmic turnover rates on 41 years versus 3 years organically managed fields indicate a stabilizing effect of organic farming on soil microflora. 相似文献
10.
A study of the effects of different qualities (fresh and composted) and rates (equivalent to 120, 240, and 360 kg N ha–1) of mustard meal application on wheat yields on humid tropical vertisol was started in 1990 at Ginchi Research Station in
Ethiopia. After continuous wheat cropping for 7 years and without any further fertilisation, soil microbial parameters (basal
respiration, microbial biomass-C and N, organic-C, and ecophysiological quotients) were studied during one growth period.
After 7 years of application, mustard meal still exerted a significant positive effect on microbial biomass, basal respiration,
organic-C, Cmic : Nmic ratio, and metabolic quotient (qCO2). Organic-C, qCO2 and Cmic : Nmic ratios were higher for the compost-amended plots than plots amended with fresh mustard meal. Basal respiration, Cmic, and Cmic : Nmic ratio showed a clear seasonality, but only in manured plots. The data indicate shifts in microbial community structure (from
bacteria to fungi and from r to K strategists) and suggest positive medium-term effects of mustard meal on humid tropical
vertisol biological qualities.
Received: 25 May 1999 相似文献
11.
Rolf Nieder Astrid Willenbockel Elisabeth Neugebauer Paul Widmer Jrg Richter 《植物养料与土壤学杂志》1995,158(5):477-484
Significance of microbial biomass and mineral fixed ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. II. 15N-turnover Field experiments 1988/89 on a fallow plot of the southern Niedersachsen loess area with straw application (δ 10 t · ha?1, homogeneously incorporated by hand) yielded an increase in microbial biomass-N (Nmic) by 60 kg N · ha?1 · 30 cm?1 until March 1989 and further 40 kg N · ha?1, till May which was almost completely remobilized until harvest. For a cropped plot (with winter wheat and 10 t · ha?1 straw incorporation), N immobilization was of similar magnitude. Up to 18% of the applied 15N-fertilizer (185 kgN · ha?1) were microbially immobilized. In contrast to 1988/89, no significant mass change of Nmic occurred in 1991 due to straw application (δ 10t · ha?1). Variations in the amount of Nmic were nearly independent on the treatment (crop, with 140 kg fertilizer-N · ha?1 or without N-fertilizer, respectively; fallow plot without fertilizer-N) within a range of 225-400 kg N · ha?1 · 30 cm?1. Directly after N-application (each 70 kg N · ha?1 in March and in May), up to 100% of the fertilizer-N were assimilated by the microbes. Subsequently, remobilization of the immobilized nitrogen occurred within 2 (in March) or 6 weeks (in May), respectively. Simultaneously, organic soil-N was mineralized after each N-application and minerally fixed for us biggest part. Between March and June, the fixed NH4+ decreased by about 112 kg · ha?1 · 30 cm?1. 相似文献
12.
Xin Zhao Yoshitaka Kakubari 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):322-332
Abstract Soil microbial biomass (Cmic) is an important factor regulating a number of ecosystem processes. In this study, we investigated seasonal variations in soil microbial biomass in natural climax beech (Fagus crenata) forests in a typical cold-temperate mountain region of Japan. Four permanent tower sites along an altitudinal gradient were selected and soil samples were collected once every month during the growing season of 2007. Soil microbial biomass (by fumigation-extraction method) and soil properties were later measured in the laboratory, while environmental factors (soil temperature, soil moisture) were continuously recorded in the field. Our results indicated large seasonal variations (130.4 ~ 5558.0 µg g?1) in soil microbial biomass in beech forests – a range that is much larger than previously reported. Statistically significant correlations are noted between soil properties with Cmic, but largely due to spatial linkages. On the other hand, the environmental factors of soil temperature and especially soil moisture largely control seasonal variations in Cmic. Furthermore, pH could be an important factor influencing seasonal change in Cmic at the 20–30 cm deep soil layer. The study suggests no direct correlation between plant eco-physiology and soil microbial biomass in seasonal courses of the forests. 相似文献
13.
Significance of microbial biomass and non-exchangeable ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. I. Change of pool sizes Nitrogen transformations in loess soils have been examined by laboratory and field experiments. After straw application (· 8 t · ha?1), N in microbial biomass (Nmic) increased by about 20 mg · kg?1 soil (· 90 kg N · ha?1 · 30 cm?1) after 9 days of incubation (20 °C). Another laboratory experiment yielded an increase of about 400 mg of NH4+-N · kg?1 fixed by minerals within 1 h after addition of 1 M NH4+-acetate. Defixation of the recently fixed NH4+ after addition of 1 M KCl amounted to only 60 mg · kg?1 within 50 days. In a field experiment with winter wheat 1991, an increase in Nmic of about 80 kg N · ha?1 · 30 cm?1 was observed from March to June. After July, growth of the microbes was limited by decreased soluble carbon concentrations in the rhizosphere. Different levels of mineral N-fertilizer (0, 177 and 213 kg N · ha?1) did not affect significantly the microbial biomass. The same field experiment yielded a decrease of non-exchangeable ammonium on the “zero”-fertilized plot in spring by 200 kg N · ha?1 · 30 cm?1. The pool of fixed ammonium increased significantly after harvest. After conventional mineral N-fertilizer application (213 kg N · ha?1). NH4+-defixation was only about 120 kg N · ha?1 · 30 cm?1 until July. 相似文献
14.
The effect of increasing rates of 15N‐labelled Ca(NO3)2 (N0 = no N application, N300 = 300 mg N/pot; N600 = 600 mg N/pot; N900 = 900 mg N/pot) on recovery of fertilizer N in winter wheat plants and soil (total soil N, soil microbial biomass N [Nmic], extractable organic N [Norg]) and on N mineralization (NMsoil) was investigated at milk‐ripe growth stage in a pot experiment. The N rates were equally split at tillering, stem elongation and ear emergence. Fertilizer N recovered in crops increased with increasing N rates (N300: 223.5 mg N/pot [74.5% of applied fertilizer N], N600: 445.6 mg N/pot [74.3%], N900: 722.1 mg N/pot [80.2%]). NMsoil slightly increased from N0 (43.8 mg N/pot) to N900 (75.6 mg N/pot) indicating that N application enhanced availability of soil‐derived N for the plants. However, in fertilized treatments NMsoil is balanced by immobilization and losses (non‐recovered fertilizer N). Therefore the effective soil N mineralization is indicated by apparent net N mineralization (ANNM = NMsoil — fertilizer N immobilization — lost fertilizer N). Fertilizer N immobilization in soil increased from N300 (38.7 mg N/pot) to N600 (60.7 mg N/pot) and N900 (65.5 mg N/pot). Lost fertilizer N increased from N300 (14.8 mg N/pot) to N600 (56.7 mg N/pot) and N900 (62.1 mg N/pot). As a consequence negative ANNM values were calculated at N600 and N900. Due to the small differences between N600 and N900 fertilizer N immobilization and lost fertilizer N did not increase linearly with increasing N rates, i.e. both processes were limited by factors other than N rate. Only 5.6—7.4% of the immobilized fertilizer N was recovered in Norg and 5.4—9.3% in Nmic soil pools. It is assumed that most of the immobilized fertilizer N was in non‐extractable organic N forms. Nmic and Norg were weak indicators for the extent of fertilizer N immobilization. 相似文献
15.
Muneshwar Singh Samaresh Kundu Ashis K. Biswas Jayanta K. Saha Awadhesh K. Tripathi Chuni L. Acharya 《植物养料与土壤学杂志》2004,167(5):577-583
A computational exercise was undertaken to quantify the percent N derived from atmosphere %Ndfa) in soybean and consequent N benefit from biological N2‐fixation process annually accrued to the soil by the soybean crop using average annual N‐input/‐output balance sheet from a 7 yr old soybean‐wheat continuous rotational experiment on a Typic Haplustert. The experiment was conducted with 16 treatments comprised of combinations of four annual rates of farmyard manure (FYM ? 0, 4, 8, and 16 t ha–1) and four annual rates of fertilizer N (? 0, 72.5, 145, and 230 kg N ha–1) applications. The estimated N contributed through residual biomass of soybean (RBNS) consisting of leaf fall, root, nodules, and rhizodeposition varied in the ranges of 7.02–16.94, 11.65–28.83, 3.31–8.91, and 11.3–23.8 kg N ha–1 yr–1, respectively. A linear relationship was observed between RBNS and harvested biomass N (HBNS) of soybean in the form of RBNS = 0.461 × HBNS – 20.67 (r = 0.989, P < 0.01), indicating that for each 100 kg N assimilated by the harvested biomass of soybean, 25.4 kg N was added to the soil through residual biomass. The Ndfa values ranged between 13% and 81% depending upon the annual rates of application of fertilizer N and FYM. As per the main effects, the %Ndfa declined from 76.4 to 26.0 with the increase in annual fertilizer‐N application from 0 to 230 kg N ha–1, whereas %Ndfa increased from 40.8 to 65.8 with the increase in FYM rates from 0 to 16 t ha–1, respectively. The N benefit from biological N2 fixation accrued to the soil through residual biomass of soybean ranged from 7.6 to 53.7 kg N ha–1 yr–1. The treatments having %Ndfa values higher than 78 showed considerable annual contribution of N from N2 fixation to the soil which were sufficient enough to offset the quantity of N removed from the soil (i.e., native soil N / FYM‐N / fertilizer‐N) with harvested biomass of soybean. 相似文献
16.
The impacts of crop rotations and N fertilization on microbial biomass C (Cmic) and N (Nmic) were studied in soils of two long-term field experiments initiated in 1978 at the Northeast Research Center (NERC) and in
1954 at the Clarion-Webster Research Center (CWRC), both in Iowa. Surface soil samples were taken in 1996 and 1997 from plots
of corn (Zea mays L.), soybeans (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) that had received 0 or 180 kg N ha–1 before corn and an annual application of 20 kg P and 56 kg K ha–1. The Cmic and Nmic values were determined by the chloroform-fumigation-extraction method and the chloroform-fumigation-incubation method, respectively.
The Cmic and Nmic values were significantly affected (P<0.05) by crop rotation and plant cover at time of sampling, but not by N fertilization. In general, the highest Cmic and Nmic contents were found in the multicropping systems (4-year rotations) taken in oats or meadow plots, and the lowest values
were found in continuous corn and soybean systems. On average, Cmic made up about 1.0% of the organic C (Corg), and Nmic contributed about 2.4% of the total N (Ntot) in soils at both sites and years of sampling. The Cmic values were significantly correlated with Corg contents (r≥0.41**), whereas the relationship between Cmic and Ntot was significant (r≤0.53***) only for the samples taken in 1996 at the NERC site. The Cmic : Nmic ratios were, on average, 4.3 and 6.4 in 1996, and 7.6 and 11.4 in 1997 at the NERC and CWRC sites, respectively. Crop rotation
significantly (P<0.05) affected this ratio only at the NERC site, and N fertilization showed no effect at either site. In general, multicropping
systems resulted in greater Cmic : Corg (1.1%) and Nmic : Ntot (2.6%) ratios than monocropping systems (0.8% and 2.1%, respectively).
Received: 9 February 1999 相似文献
17.
Bachir Bounou Issoufa Robert Clement Abaidoo Nana Ewusi-Mensah 《Archives of Agronomy and Soil Science》2019,65(8):1107-1119
A two-year field experiment was conducted in Niger to explore the effects of integrated use of millet glume-derived compost (MGD-Compost) and NP fertilizer on soil microbial biomass carbon (Cmic), nitrogen (Nmic) and millet yields. Three compost rates (3000 kg ha?1, 1500 kg ha?1 and 0 kg ha?1) and three NP fertilizer rates (100%, 50% and 0% of recommended NP fertilizer) were arranged in a factorial experiment organized in a randomized complete block design with three replications. Combined application of compost and NP fertilizer induced a synergistic effect on Cmic and Nmic. Compost application increased millet grain yield from 59% to 91% compared to control. Combined application of compost and NP fertilizer increased millet grain yields from 57% to 70% in 2013 and from 36% to 82% in 2014 compared to sole application of mineral fertilizer. Agronomic efficiency (AE) of nitrogen values increased by 3.7 and 2.3 times than those of sole NP fertilizer application in 2013 and 2014, respectively. Phosphorus AE was 1.6 times higher than that of the sole application of NP fertilizer. These findings indicate that integrated application of MGD-Compost and NP fertilizer enhances soil microbial biomass content and increases millet grain yield in a low-input cropping system. 相似文献
18.
Dirk Landgraf 《植物养料与土壤学杂志》2001,164(6):665-671
The response of microbial biomass carbon (Cmic), nitrogen (Nmic), basal respiration, and the metabolic quotient to 3 years of a natural succession fallow were studied in a field experiment on sandy soil in Northeast Saxony/Germany from 1996 to 1998. Soil samples were taken from Eutric Cambisol and Mollic Cambisol every six weeks during the vegetation period at soil depths of 0—10 and 10—30 cm. The Cmic content in the topsoils increased with time of succession in both soil types. This trend was more distinct in the Mollic Cambisol (70.7 μg g—1 in June 1996 to 270.9 μg g—1 in October 1998 at 0—10 cm) than in the Eutric Cambisol (69.7 μg g—1 in June 1996 to 175.0 μg g—1 in October 1998 at 0—10 cm). By contrast, the Nmic content slightly decreased in the Eutric Cambisol from 18.9 μg g—1 to 17.7 μg g—1 during the same time period. In the Mollic Cambisol, the Nmic increased from 18.8 μg g—1 in spring 1996 to 35.5 μg g—1 in fall 1998, however to a lower extent than the Cmic. Subsequently, the (C:N)mic ratio increased from 4.3 to 5.8 at soil depth of 0—10 cm and from 3.5 to 6.5 at 10—30 cm during the 3‐year‐study at the Eutric Cambisol. In the Mollic Cambisol, the enhancement of (C:N)mic ratio was more pronounced (i.e. from 4.3 to 6.7 at 0—10 cm and from 3.5 to 7.2 at 10—30 cm). Most likely this results from a shift in microbial populations towards a dominance of soil fungi. The already low basal respiration of, on average, 0.26 mg CO2 g—1 (24h)—1 (0—10 cm) in June 1996 decreased with time of succession fallow to 0.15 and 0.22 mg CO2 g—1 (24h)—1 in October 1998 in the Eutric and the Mollic Cambisol, respectively. Thus, the metabolic quotient as an indicator for the efficiency of organic matter turnover in soil was very low in both soils. During the summer months, the metabolic quotients reached minimum levels of ≤ 0.1 μg CO2 C (g Cmic)—1 h—1, probably because of low soil moisture contents. Correlation analyses revealed close relationships between Nmic and total N, Nmic and water content, and Nmic and pH values. These relationships became even more pronounced with the time period of natural succession. For the samples from fall 1998, highly significant correlations were determined between Nmic and total N (coefficients were rs = 0.91***), Nmic and water content (rs = 0.91***), and Nmic and pH value (rs = 0.76***). The values for all biological parameters studied were larger in the Mollic than in the Eutric Cambisol. This indicates higher turnover rates of different C and N fractions in the Mollic Cambisol. In general, set aside of formerly agricultural managed sandy soils resulted in greater Cmic : Nmic ratios and thus, in a change in the microbiological community structure as well as in reduced C and N turnover rates (i.e. low metabolic quotient) under the climatic conditions of the East German lowlands. 相似文献
19.
《European Journal of Soil Biology》2006,42(1):1-12
The purpose of this study was to measure the effects of plant growth on soil microbial biomass C (Cmic) and soil enzyme activities. In a pot experiment using spring barley and sugar beet, we investigated the response of Cmic, hot water extractable C and N fractions (Chwe, Nhwe), and enzyme activities involved in C, N and P cycling in a loess-derived Chernozem from Bad Lauchstädt (Central Germany). The study site has been receiving the same fertilisation treatments for 100 years. The soil originated from plots fertilised with 15 t ha−1 farmyard manure (FYM) year−1 + mineral fertiliser (NPK), or 15 t ha−1 FYM year−1, or NPK or from an unfertilised control. Pots were sampled monthly, and alkaline phosphatase- (AP), β-glucosidase- and protease-activities were analysed. At the beginning and the end of study, levels of Cmic, Chwe and Nhwe were also measured. All three enzyme activities and Cmic were significantly and positively correlated with Corg and Chwe. Results suggest that the enzyme activities measured originated mostly from microorganisms and that Chwe is an important C source for soil microorganisms. β-Glucosidase and AP activities were higher in summer months than at other times. In contrast, protease activity changed only slightly during the growing period. 相似文献
20.
Plant canopy effects on litter accumulation and soil microbial biomass in two temperate forests 总被引:2,自引:0,他引:2
The objective of this study was to determine whether differences in canopy structure and litter composition affect soil characteristics
and microbial activity in oak versus mixed fir-beech stands. Mean litter biomass was greater in mixed fir-beech stands (51.9t
ha−1) compared to oak stands (15.7t ha−1). Canopy leaf area was also significantly larger in mixed stands (1.96m2 m−2) than in oak stands (1.73m2 m−2). Soil organic carbon (C
org) and moisture were greater in mixed fir-beech stands, probably as a result of increased cover. Soil microbial biomass carbon
(C
mic), nitrogen (N
mic), and total soil nitrogen (N
tot) increased slightly in the mixed stand, although this difference was not significant. Overall, mixed stands showed a higher
mean C
org/N
tot ratio (22.73) compared to oak stands (16.39), indicating relatively low rate of carbon mineralization. In addition, the percentage
of organic C present as C
mic in the surface soil decreased from 3.17% in the oak stand to 2.26% in the mixed stand, suggesting that fir-beech litter may
be less suitable as a microbial substrate than oak litter. 相似文献