盆栽微型月季离体培养繁殖技术探讨

以6个盆栽微型月季品种为试验材料进行离体培养，以MS为基本培养基附加不同浓度的6-BA、NAA，以诱导腋芽增殖为繁殖途径。研究结果表明，最适诱导培养基为MS＋6-BA（0．5～1．0mg／L）；以茎段为继代材料的增殖系数均优于以顶芽为继代材料的增殖系数，同时顶芽的继代增殖系数以外源激素组合6-BA（1mg／L）＋NAA（0．01mg／L）为优，而茎段的继代增殖系数以外源激素组合6-BA（1．5mg／L）＋NAA（0．05mg／L）为优；壮芽培养基为Ms＋6．BA（0．5mg／L）＋NAA（0．2mg／L）；生根培养基为1／2MS＋NAA（0．03～0．1mg／L）。     

果蔗脱毒组培苗速生配套栽培初探

果蔗脱毒组培苗经过速生配套栽培管理,株高、有效茎优于第1代健康种茎,糖度、茎径和产量与第1代健康种茎相当;株高、茎径和产量均明显高于果蔗脱毒组培苗常规栽培。通过速生配套栽培,茎尖脱毒组培苗能当年种植当年采收上市,不仅运输方便、栽培简单,还能提前上市。     

油菜轻简栽培技术的研究进展

油菜轻简栽培是油菜生产发展的必然趋势,对其进展情况进行了概述,阐明了几种轻简栽培方式的特点及技术关键,分析了存在的问题,并提出了今后研究方向。     

Cytopathic effect of canine distemper virus in tissue culture

A characteristic cytopathic effect was obtained with canine distemper virus (Onderstepoort strain) propagated in chick embryo fibroblast monolayer tissue culture. In limiting dilutions the lesions were focal. The titer at the 20th tissue culture passage was approximately 10(5) TCD(50)/ml. Cytopathogenicity was specifically inhibited by distemper immune serum. Minute plaques were produced under agar overlay.     

杂交春兰未成熟种子无菌培养的研究初报

以普通蕙兰×春兰(宋梅)、春兰(小打梅)×春兰(绿壳素)、春兰(外碟)×春兰(大富贵)组合为材料,进行了成熟和未成熟杂交种子(胚)无菌培养的研究。结果表明,未成熟种子(授粉后100 d左右)发芽率高于成熟种子,加活性碳的培养基发芽速度明显比未加活性碳的慢但发芽率高。     

慈姑轻简栽培技术规程

慈姑轻简栽培技术是以慈姑基质育苗技术为核心,通过培育健壮慈姑种苗、结合旱田定植,简化慈姑种植,节约人工,提高生产效率。本文从产地环境、品种选择、基质育苗、定植、田间管理、病虫害防治及采收等方面总结了慈姑轻简栽培技术规程,以期为慈姑轻简化栽培技术推广应用提供技术支持。     

Radiation leukemia virus: quantitative tissue culture assay

Radiation leukemia virus does not propagate in tissue cultures from either Swiss or C57BL mouse embryos, but it does augment focus formation by the defective Moloney leukemia pseudotype of murine sarcoma virus in Swiss mouse cells and thus can be quantitatively assayed.     

大叶藻组织培养中外植体消毒方法的研究

为探寻大叶藻组织培养中外植体的最佳消毒方法,采用75%乙醇、1.5%碘化钾、4%次氯酸钠和10%双氧水这4种消毒剂以及不同的处理时间组合,对大叶藻种子和幼苗进行消毒试验。试验结果,最有效的外植体消毒方法为：对大叶藻幼苗,先用无菌水冲洗3~4次,再以75%乙醇处理20 s,然后再用无菌水冲洗3~4次,可以达到较好的消毒效果。对大叶藻种子,先以75%乙醇处理10 min,再以1.5%碘化钾处理10 min,然后用无菌海水冲洗3~4次,这样消毒效果最佳,且不影响种子的发芽率。     

稻米直链淀粉含量简化测定方法的试验研究

提出了一种基于计算机色度学原理的稻米直链淀粉含量检测方法。探讨了利用扫描仪代替传统的分光光度计 ,通过一次性采集样液的色度值 ,同时测定多个稻米样品的直链淀粉含量 ,并自动确定其所属等级的可能性。研究结果表明 ,显色液的色度值与直链淀粉质量浓度具有良好的线性相关性 (相关系数为 0 980 ) ,所提出的新型直链淀粉含量简化测定方法具有成本低、效率高、对环境条件的要求相对宽松等特点 ,为GB/T 17891— 1999　《优质稻谷》在粮食收购中的应用提供了依据     

甘薯病毒检测技术的改进与组培苗病毒分布特征研究

用NCM-ELISA方法对不同来源的甘薯样品进行6种病毒的检测，结果发现：在农户样品中，以SPFMV的发病最重，检出率高达90％；脱毒后的第一代则以次要病毒检出率为高。同时对三种不同制样方法进行对比，发现以叶柄提取液检出率（66.6％）为最高，比叶片提取液检出率高55.5个百分点。对试管苗进行分节检测的结果表明，病毒分布呈从基部至顶部逐步递减的基本规律，因此在进行试管苗扩繁时，应取顶部以下1-2节，而在进行病毒检测时，则应取靠近培养基的1-2节。     

台湾青枣组织培养的消毒方法

为了确立台湾青枣组织培养的消毒方法 ,研究了多菌灵、酒精、升汞和吐温 2 0这 4种消毒剂对台湾青枣组织培养的消毒效果和对其愈伤组织生长的影响。结果表明 :提前 5d喷 6 0 0倍液多菌灵 ,能明显减少污染率 ;经 30s75 %酒精消毒后 ,再用 0 1%升汞加 3～ 5滴吐温 2 0消毒 5min ,既能减少污染 ,又能促进外植体愈伤组织生长。     

Significance, progress and prospects for research in simplified cultivation technologies for rice in China

Simplified cultivation technologies for rice have become increasingly attractive in recent years in China because of their social, economical and environmental benefits. To date, several simplified cultivation technologies, such as conventional tillage and seedling throwing (CTST), conventional tillage and direct seeding (CTDS), no-tillage and seedling throwing (NTST), no-tillage and direct seeding (NTDS) and no-tillage and transplanting (NTTP), have been developed in China. Most studies have shown that rice grown under each of these simplified cultivation technologies can produce a grain yield equal to or higher than traditional cultivation (conventional tillage and transplanting). Studies that have described the influences of agronomic practices on yield formation of rice under simplified cultivation have demonstrated that optimizing agronomy practices would increase the efficiencies of simplified cultivation systems. Further research is needed to optimize the management strategies for CTST, CTDS and NTST rice which have developed quickly in recent years, to strengthen basic research for those simplified cultivation technologies that are rarely used at present (such as NTTP and NTDS), to select and breed cultivars suitable for simplified cultivation and to compare the practicability and effectiveness of different simplified cultivation technologies in different rice production regions.     

杂种补血草组织培养与栽培技术

利用杂种补血草品种"兰雾"种子为外植体,在不同激素水平的培养基上诱导产生愈伤组织、丛生芽和根,完成植株再生。研究发现,其愈伤组织产生的芽的分化速度较快。最后移入生根培养基上长成完整植株。同时对栽培管理技术还进行了研究和探讨。