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1.
Interspecific hybridization has been successful between two economically important species of Jatropha, viz., J. curcas and J. integerrima. The interspecific hybrids exhibited morphological intermediacy for various vegetative characteristics but produced flowers with three distinct colours. Backcrossing of the F1 hybrids resulted in a number of flower colours varying from dark pink through green to white enhancing the ornamental value of the genus. Hybridity was confirmed through PCR amplification using random primers. The various propagation methods for these new ornamental Jatrophas are discussed. 相似文献
2.
Vinod Kumar Yadav Sandeep Kumar Ravindra K. Panwar 《Genetic Resources and Crop Evolution》2007,54(6):1285-1289
The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken,
out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic
similarities of the RAPD profiles were estimated by using Jaccard’s coefficient with NTSYSpc 2.0 software. The similarity
index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram
generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes
and major Group B have nine genotypes. 相似文献
3.
Prabalee Sarmah P. K. Barua R. N. Sarma P. Sen P. C. Deka 《Genetic Resources and Crop Evolution》2007,54(3):593-600
Southeast Asia hosts a great diversity of different rattan genotypes. There are 5 genera and 60 different species of rattan
in India and the Northeastern region is a natural sanctuary for 4 different genera and 16 different species. The natural reserves
of this species have come under the threat of genetic erosion due to overexploitation. This investigation was directed at
characterizing 15 rattan genotypes of the genera Calamus, Plectocomia and Daemonorops which yield rattans of commercial importance, based on RAPD fingerprints. From 20 different random decamer primers, 12 primers
gave reproducible amplification profiles and 104 polymorphic bands. A considerable degree of polymorphism (98.1%) was detected
among the genotypes. Jaccard’s coefficient of similarity ranged from 0.146 to 0.632 with a mean of 0.320±0.082, signifying
extensive genetic divergence among the genotypes studied. UPGMA cluster analysis clearly distinguished P. assamica Griff. and C. erectus Roxb. The other 13 genotypes were grouped into two distinct clusters, one cluster involving two C. tenuis genotypes along with C. inermis T.Anders. and C. acanthospathus Griff. and the other cluster involving the rest of the Calamus genotypes along with D. jenkinsianus (Griff.) Mart. Unique fingerprints for 7 Calamus and 1 Daemonorops genotypes were detected. The results presented in this paper demonstrated the utility of RAPD markers in elucidating patterns
of genetic variation among genotypes of the three main rattan genera of Northeast India and in identifying individual genotypes,
which may serve as potential sources of unique genetic material for genetic improvement and conservation. 相似文献
4.
Saowaluck Bua-in Yingyong Paisooksantivatana 《Genetic Resources and Crop Evolution》2010,57(3):405-414
The genetic relatedness among 51 accessions, 14 species of the genus Zingiber and genetic variability of a clonally propagated species, Zingiber montanum (Koenig) Link ex Dietr., from Thailand were studied using random amplified polymorphic DNA (RAPD) profiles. Twenty-nine random
primers gave reproducible amplification banding patterns of 607 polymorphic bands out of 611 scored bands accounting for 99.40%
polymorphism across the genotypes. Jaccard’s coefficient of similarity varied from 0.119 to 0.970, indicative of distant genetic
relatedness among the genotype studied. UPGMA clustering indicated eight distinct clusters of Zingiber, with a high cophenetic correlation (r = 1.00) value. Genetic variability in Z. montanum was exhibited by the collections from six regions of Thailand. High molecular variance (87%) within collection regions of
Z. montanum accessions was displayed by AMOVA and also explained the significant divergence among the sample from six collection regions.
Our results indicate that RAPD technique is useful for detecting the genetic relatedness within and among species of Zingiber and that high diversity exists in the clonally propagated species, Z. montanum. 相似文献
5.
Genetic diversity of seven Rhus L. species was assessed using random amplified polymorphic DNA (RAPDs) markers. Initially, 90 primers were screened, of which 25 produced reproducible amplification products. These primers generated a total of 296 bands, with an average of 11.8 bands per primer. Out of 296 bands scored, 236 (80%) were polymorphic and 62 (20%) were monomorphic. Primers OPC-05 and OPD-05 generated 100% polymorphic bands. The resolving power of primers ranged from 9.4 to 26.8. Similarity matrix values ranged from 0.45 to 0.63. The dendrogram generated using Unweighted Pair Group Method using Arithmetic Averages (UPGMA) grouped all the species of Rhus in one major group with two sister groups, whilst R. pyroides Burch. and R. dentata Thunb. were outliers. R. gerrardii (Harv. ex Engl.) Diels, R. glauca Thunb. and R. pentheri Zahlbr. constituted one sister group, while R. natalensis Bernh. ex C. Krauss and R. gueinzii Sond. were included in the other. The degree of genetic diversity observed between seven species of Rhus with RAPD markers suggest that this approach could be used for studying the phylogeny of the genus. 相似文献
6.
Alagarsamy Karthikeyan Anandaraj Madhanraj Shunmugiah Karutha Pandian Manikandan Ramesh 《Genetic Resources and Crop Evolution》2011,58(5):769-782
Randomly amplified polymorphic DNA (RAPD) fingerprinting approach was applied to assess genetic diversity in different accessions
of rejuvenating and intellect-promoting ancient ayurvedic medicinal herb Bacopa monnieri (L.) Pennell collected from four Southern Indian states, along with in vitro micropropagated samples maintained in the laboratory
for 5 years. With the 10 analyzed primers, 110 distinct bands, in the size range of 250–870 bp were observed, and among which
14 (12.72%) were polymorphic. Among the random primers used, only OPD 02 generated highest percentage of polymorphism with
30.77. Whereas OPD 08 generated a maximum of 18 amplified bands, but with only 1% polymorphism. Cluster analysis done on the
basis of similarity co-efficient generated from RAPD profiles indicated all the accessions were divided into two sub-groups
based on genetic distances under one major cluster. Major cluster is only the lone loose group of the Bm.2 collected from
Kerala (KER) and in vitro micropropagated plant (IVMP) maintained in the lab. The other sub-group consisted of Bm.1 and Bm.3
collected from Tamil Nadu (TN) and Andhra Pradesh (AP) respectively, which are genetically similar and showed similarity with
accessions from Karnataka Bm.4 (KK). These two sub-groups were joined together at 0.66 genetic distance level. Overall, the
levels of genetic similarity within the accessions varied from 0.24 to 0.80, the matrix ranged from 0.36 to 0.80, with a mean
value of 0.68 indicating genetic similarity at low level. 相似文献
7.
Mehboob-ur-Rahman Tahira Yasmin Nabila Tabbasam Ihsan Ullah Muhammad Asif Yusuf Zafar 《Genetic Resources and Crop Evolution》2008,55(3):331-339
Genetic diversity is an area of concern for sustaining crop yield. Information on genetic relatedness/diversity among Gossypium arboreum L. cultivars/genotypes is scanty. We have used random amplified polymorphic DNA (RAPD) analysis to assess the genetic divergence/relationship
among 30 genotypes/cultivars of G. arboreum. Of 45 primers surveyed, 63% were polymorphic. Out of the total number of loci amplified, 36% were polymorphic. The calculated
genetic similarity between the cultivars/genotypes was in the range of 47.05–98.73%. Two genotypes, HK-244 and Entry-17, were
the most distantly related. The average genetic relatedness among all the genotypes was 80.46%. However, most of the cultivated
varieties showed a close genetic relationship, indicating a narrow genetic base in comparison to the non-cultivated germplasm.
The calculated coefficients were used to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA)
algorithm, which grouped the genotypes/cultivars into two major and three smaller clusters. The study is the first comprehensive
analysis of the genetic diversity of G. arboreum germplasm and identifies cultivars that will be useful in extending the genetic diversity of cultivated varieties and future
genome mapping projects. 相似文献
8.
Hong Bo Guo Bao Rong Lu Qian Hong Wu Jia Kuan Chen Tong Shui Zhou 《Genetic Resources and Crop Evolution》2007,54(5):917-924
Genetic diversity of seven cultivated populations of Codonopsis pilosula Nannf. from Longxi County, Gansu Province of China was estimated using randomly amplified polymorphic DNA (RAPD) markers.
The 17 selected RAPD primers amplified 205 polymorphic bands out of a total of 235 (87.2%). Nei’s gene-diversity statistics
and population differentiation parameters based on AMOVA analysis indicated that the cultivated C. pilosula populations remained a high level of genetic diversity with Hs = 0.299 and I = 0.450. A greater proportion of genetic diversity was found within (77%) rather than among (23%) the populations. In addition,
we also detected that populations from different altitudes had a considerable genetic differentiation after 40 years of cultivation
at the same site. Populations from higher altitude had lower genetic diversity than those from lower altitude. Our results
suggested that irregular and sparse cultivation practices, i.e., random collecting, preserving, and planting seeds of the
medicinal species without deliberate selection, might be an efficient way to conserve genetic resources of medicinal plants,
in addition to their effective uses. 相似文献
9.
K. N. Ganapathy B. N. Gnanesh M. Byre Gowda S. C. Venkatesha Sunil S. Gomashe V. Channamallikarjuna 《Genetic Resources and Crop Evolution》2011,58(6):837-847
A total of 561 amplified fragment length polymorphism (AFLP) loci were generated and used to study the genetic diversity of
wild and cultivated genotypes of pigeonpea. Out of 561 marker loci, 558 were polymorphic with an average of 76.12 bands. Analysis
of molecular variation (AMOVA) revealed significant strong population structure when genotypes were structured according to
continent of origin (FST = 0.22) also when structured into cultivated and wild genotypes (FST = 0.16). Maximum polymorphic loci were observed in cultivated species C. cajan (352) which is due to more number of genotypes used while, a minimum number of 45 polymorphic bands were obtained in C. acutifolius. Highest (0.291) average gene diversity was recorded in species C. mollis and lowest (0.079) average gene diversity was recorded in C. acutifolius. The 33 genotypes grouped into 14 clusters at 26% Jaccard’s similarity coefficients. Clustering analysis revealed most cultivated
genotypes grouped into one major cluster while, the wild genotypes grouped into many clusters at 26% Jaccard’s similarity
revealing greater diversity within wild species as compared to cultivated genotypes. From among the various cultivated genotypes
studied, four genotypes BRG 3, ICP 7035, TTB 7 and ICP 8863 were molecularly and morphologically diverse and were used as
parental genotypes to study nature of inheritance and to identify markers linked to sterility mosaic disease. 相似文献
10.
The genetic diversity and similarities among 32 Kengyilia accessions, distributed to 14 species and one variety were analyzed by using random amplified microsatellite polymorphism
(RAMP) markers. Of the 160 RAMP primer combinations tested, 40 (25%) produced polymorphic and clear bands. A total of 264
bands were produced by 40 primer combinations, among which 231 out of 264 bands (87.5%) were polymorphic. Two to 11 polymorphic
bands could be amplified from each primer combination, with an average of 5.8 bands. The data of 264 bands were used for RAMP
assay. By NTSYS-pc program, genetic similarity coefficients were generated and dendrogram was constructed using UPGMA. The
genetic similarity coefficients ranged from 0.477 to 0.965 with the mean of 0.714. The results showed as follows: (1) distinct
genetic differences were present among the different species; (2) the different accessions in a species were clustered together,
respectively, which had larger genetic similarities and closer relations; (3) the species with similar morphological characters
and the species from the same areas or neighboring geographical regions were clustered together; (4) the lowest genetic similarity
was found between K. hirsuta (PI531618) and K. laxiflora (PI531631), while the highest genetic similarity was observed between K. hirsuta (Y2364) and K. hirsuta (Y2368); (5) RAMP results are basically comparable with those obtained from studies on morphology and cytology. It is a useful
method for analysis of the genetic diversity and similarities in Kengyilia. 相似文献
11.
Atsushi Murakami Peter Darby Branka Javornik Maria S. S. Pais Elisabeth Seigner Anton Lutz Petr Svoboda 《Genetic Resources and Crop Evolution》2006,53(8):1553-1562
To study the relationships and genetic diversity among wild hops, Humulus lupulus, we analyzed 133 samples of wild hops collected from Europe, Asia and North America using polymorphism on 11 microsatellite
loci. Although only three primers showed bands in Japanese hops, all other samples showed polymorphic bands at most loci.
There were no duplicate genotypes among samples of European, Chinese and North American hops, and each individual hop could
be distinguished completely. The phylogenetic tree constructed from DA distance with the UPGMA method showed a large cluster
comprised of European hops, although Russian hops from the Caucasus and Altai regions were separate from the European cluster.
Chinese and North American samples gave distinct clusters suggesting genetic differentiation. This study has indicated that
hop microsatellite DNA is differentiated, and is dependent upon the origin in regions of Europe, Asia and North America. 相似文献
12.
The genetic diversity and the relationships among a collection of Brassica napus L. European populations were evaluated using random amplified polymorphic DNA markers. The study included 33 accessions of
B. napus collected from Galicia (northwestern Spain) and 18 British cultivars, 16 accessions of B. napus and two accessions of Brassica oleracea L. used as controls. DNA from 25 individuals per population was analyzed using 18 decamer primers. One hundred thirty-eight
amplification products were scored of which 105 were polymorphic. These bands ranged in size from 350 to 2500 base pairs.
Similarity coefficients and cluster analysis were computed and six groups were obtained. Cluster I was the largest and included
all the landraces from northwestern Spain, except two accessions that grouped separately into Clusters III and IV, respectively.
A low level of genetic variability was detected among the B. napus Spanish genotypes, while considerable diversity was present among the British ones, which grouped into three groups, two
main clusters and one group formed by one accession. Cluster II included all commercial varieties grown in Great Britain whereas
Cluster V grouped local varieties maintained by the growers for many years. Cluster VI was a singularity formed by one entry.
British accessions of B. oleracea had the greatest dissimilarity with all the other populations and grouped separately in Clusters VII and VIII. As conclusion,
B. napus landraces used in northwestern Spain as leafy-green vegetable probably have an independent origin from B. napus crops grown in other European regions. Besides, separate domestication in northwestern Spain and Great Britain for a different
end use might have led to two distinct gene pools. 相似文献
13.
Y. Belaïd N. Chtourou-Ghorbel M. Marrakchi N. Trifi-Farah 《Genetic Resources and Crop Evolution》2006,53(7):1413-1418
In order to evaluate the genetic diversity in Lathyrus genus, the Inter Simple Sequence Repeats method (ISSR) was exploited in five populations. These consisted of two cultivated
species belonging to section Lathyrus (L. sativus L. and L. cicera L.) and a wild one belonging to the section Clymenum (L. ochrus DC.). Two 3′anchored ISSR primers and two unanchored ones, generated a total of 60 useful polymorphic DNA bands. Our data
provide evidence of high molecular polymorphism at the intra- and the inter-specific levels showing that both wild and cultivated
forms constitute an important pool of diversity. Moreover, among the generated DNA bands, a 500 bp band, totally absent in
the banding patterns of the section Clymenum, appears to be a molecular marker of section Lathyrus. Results provided for lineage
and suggest recent origin of these species that might have evolved from a common ancestor producing both L. ochrus species and the two other species L. sativus and L. cicera. These relationships support previous studies based on morphological variation and molecular analysis. 相似文献
14.
Morphological and molecular diversity analysis among the Indian clones of <Emphasis Type="Italic">Sesuvium portulacastrum</Emphasis> L. 总被引:1,自引:1,他引:0
Vinayak H. Lokhande Tukaram D. Nikam Vikas Y. Patade Penna Suprasanna 《Genetic Resources and Crop Evolution》2009,56(5):705-717
Sesuvium portulacastrum L. (seapurslane) is a halophyte used as pioneer species in sand dune fixation and stabilization of saline soil. Studies on
the morphological and molecular diversity were carried out for the 14 clones of Sesuvium collected from the different coastal regions of India. Significant differences were observed for morphological traits viz.,
length, width, diameter and area of leaf, internodal distance and stem diameter for different clones when compared with the
clone from Gujarat state (GJ1). A UPGMA dendrogram for morphological traits based on the Pearson’s similarity coefficient
clustered the clones into three groups considering 80% polymorphism as criteria. Molecular diversity among the clones was
studied using Randomly Amplified Polymorphic DNA (RAPD), Internal Transcribed Spacer (ITS) and markers specific to Ac homologous region. Of the total 749 RAPD loci amplified with 70 random primers, 294 were polymorphic with 39.25% diversity.
A phylogenetic tree constructed with UPGMA and SHAN, grouped the clones into three major clades based on RAPD data. The molecular
diversity studied with ITS and markers specific to Ac homologous region revealed 37.50% and 66.66% polymorphism and clustered the clones into three and four clades, respectively.
The genetic diversity analysis revealed wide variations among the S. portulacastrum clones, reflecting a high level of diversity within the species which might be due to anthropogenic impact and geographic
environmental conditions. Further, the various clones from the different eco-geographic coastal localities might have originated
from native places of wild abundance. To the best of our knowledge, this is the first attempt to evaluate both morphological
and genetic diversity among the Sesuvium clones collected from the distant habitats of the coastal regions of the India. 相似文献
15.
K. Vijayan A. Tikader P. K. Kar P. P. Srivastava A. K. Awasthi K. Thangavelu B. Saratchandra 《Genetic Resources and Crop Evolution》2006,53(5):873-882
In order to formulate appropriate strategies for the conservation and utilization of the wild mulberry genetic resources available
in India, a study was undertaken with 20 mulberry genotypes from the four different species. Seventeen intersimple sequence
repeat primers were used to generate a total of 114 markers, of which 98 (85.96%) were polymorphic. Seven unique bands for
Morus serrata Roxb. and one for both M. serrata Roxb. and Morus macroura Miq. were identified, of which one fragment has been sequenced and deposited in the EMBL-GeneBank (AJ-585512). The genetic
dissimilarity coefficients varied from 0.078 to 0.530 among these genotypes and from 0.168 to 0.465 among the species. The
dendrograms realized from these markers clustered the genotypes into three groups. The outermost group was M. serrata Roxb., which was followed by the group of M. macroura Miq. and the innermost group contained genotypes of Morus indica L. and Morus alba L. This intermixing of genotypes of M. indica and M. alba supports the view that M. indica is merely a synonym of M. alba. Distribution of the genotypes on a two-dimensional figure upon multidimensional scaling with ALSCAL program, further, confirmed
the genetic divergence between the cultivated and wild mulberry groups. On the basis of the results a few potential wild mulberry
genotypes were identified for its conservation and utilization in breeding programs to confer the stress tolerance to the
cultivated varieties of mulberry. 相似文献
16.
Sezai Ercisli Emine Orhan Ahmet Esitken Nalan Yildirim Guleray Agar 《Genetic Resources and Crop Evolution》2008,55(4):613-618
Turkey is an important producer of cornelian cherries (Cornus mas L.), especially in northern Anatolia. Seed propagation and long-term human selection has given rise to a great diversity
of trees. Twenty-six cornelian cherry genotypes (CC1–CC26) from the Coruh Valley in northern Anatolia were evaluated for genetic
relationships by using Randomly Amplified Polymorphic DNA (RAPD) markers, based on 56 decamer random primers, seven of which
showed reliable polymorphisms. These seven primers generated 80 markers, with 77 (96.25%) displaying polymorphisms. Cluster
analysis of the cornelian cherry genotypes was performed based on data from polymorphic RAPD bands, by using Jaccard’s similarity
coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. A similarity matrix showed
that the highest genetic similarity (0.913) was between CC15 and CC16 and the least (0.129) was between CC4 and CC16. The
cophenetic correlation coefficient between the similarity matrix and the cophenetic matrix of the dendrogram was relatively
high (r = 0.87), supporting the validity of the dendrogram. Based on these results, RAPD analysis can be used for the characterization
and grouping of cornelian cherry genotypes. Genetically divergent genotypes identified in this study may be useful for future
breeding programs. This is the first study demonstrating that RAPD analyses can be used to differentiate and classify cornelian
cherry genotypes. 相似文献
17.
Xingquan Zeng Yajuan Wang Weiyan Li Changyou Wang Xinlun Liu Wanquan Ji 《Genetic Resources and Crop Evolution》2010,57(8):1141-1150
In order to evaluate and compare the germplasm resources of wheat in Tibet, we analyzed the genetic diversity of 136 Triticum aestivum ssp. tibetanum Shao and 119 Tibetan wheat landraces (Triticum aestivum L.) by using Intron-Splice Junction (ISJ) primers. The results showed that polymorphism of PCR products were obtained by
33 primer combinations, which accounted for 11% of the 300 primer combinations produced by 26 ISJ primers. A total of 333
stable bands can be amplified from the T. aestivum ssp. tibetanum Shao and 243 bands were polymorphic, which accounted for 72.9% of the total bands. Tibetan wheat Landraces produced 316 stable
bands, of which 197 bands were polymorphic. The polymorphic bands accounted for 62.34% of the total bands produced from Tibetan
wheat landraces. The genetic diversity of T. aestivum ssp. tibetanum Shao was higher than that of Tibetan wheat landraces in Tibet, suggesting that T. aestivum ssp. tibetanum Shao can be used as important genetic resource for the breeding and genetic improvement of wheat in Tibet. Matrix (1, 0)
was generated according to the presence or absence of the bands produced from a particular wheat accession. Clustering and
principle coordinates analysis showed that T. aestivum ssp. tibetanum Shao and Tibetan wheat landraces were divided into two groups. We conclude that high polymorphisms produced by ISJ primers
can reflect the genetic diversity between T. aestivum ssp. tibetanum Shao and Tibetan wheat landraces. 相似文献
18.
Sheji Mary N. V. Nair Pankaj Kumar Chaturvedi A. Selvi 《Genetic Resources and Crop Evolution》2006,53(6):1221-1231
Saccharum spontaneum L. a wild relative of sugarcane is the most variable and diverse among the Saccharum species. This species had been successfully exploited in sugarcane improvement programmes since 1915 and most of the present day commercial varieties are derivatives of interspecific hybrids involving S. spontaneum. The S. spontaneum germplasm available today in the World collections is diverse and represent different geographical groups. In the present investigation, an attempt was made to characterize 40 S. spontaneum clones collected from 4 different geographical areas in India using 20 random, 2 ISSR and 2 telomere primers. Of the 491 bands generated by these primers 83.9% were polymorphic. The genetic diversity estimated based on these markers was found to be moderate (48.9%). The pair-wise genetic distance between the accessions ranged from 29.8 to 60.0. The accessions from Arunachal Pradesh were found to be the most diverse, while Tamil Nadu accessions showed relatively less diversity. Diversity between Tamil Nadu and Kerala collections was found to be low, while the diversity between the Orissa group and the rest was found to be high. The collections from Mayurbanj and Cuttack regions of Orissa were found to be distinct. Arunachal Pradesh accessions, being more diverse, are a potential source for exploitation in sugarcane breeding programmes. 相似文献
19.
Amplified fragment length polymorphism markers were used to assess the genetic relatedness between Dioscorea alata and nine other edible Dioscorea. These species include D. abyssinica Hoch., D. bulbifera L., D. cayenensis-rotundata Lamk. et Poir., D. esculenta Burk., D. nummularia Lam., D. pentaphylla L., D. persimilis Prain. et Burk., D. transversa Br. and D. trifida L. Four successive studies were conducted with emphasis on the genetic relationship within D. alata and among species of the Enantiophyllum section from Vanuatu. Study 1 was carried out to select a set of polymorphic primer
pairs using 11 combinations and eight species belonging to five distinct sections. The four most polymorphic primer pairs
were used in study 2 among six species of the Enantiophyllum section. Study 3 focussed mainly on the genetic relationship
among 83 accessions of D. alata, mostly from Vanuatu (78 acc.) but also from Benin, Guadeloupe, New Caledonia and Vietnam. The ploidy level of 53 accessions
was determined and results indicated the presence of tetraploid, hexaploid and octoploid cultivars. Study 4, included 35 accessions
of D. alata, D. nummularia and D. transversa and was conducted using two primer pairs to verify the taxonomical identity of the cultivars `langlang', `maro' and `netsar'
from Vanuatu. The overall results indicated that each accession can be fingerprinted uniquely with AFLP. D. alata is an heterogeneous species which shares a common genetic background with D. nummularia and `langlang', `maro' and `netsar'. UPGMA cluster analysis revealed the existence of three major groups of genotypes within
D. alata, each assembling accessions from distant geographical origins and different ploidy levels. The analysis also revealed that
`langlang', `maro' and `netsar' clustered together with the cultivar `wael' (D. transversa) from New Caledonia. Results are discussed in the paper. 相似文献
20.
Inter Simple Sequence Repeat (ISSR) Analysis of Diploid Coffee Species and Cultivated Coffea arabica L. from Tanzania 总被引:1,自引:0,他引:1
Inter simple sequence repeat (ISSR) markers were used to evaluate levels of genetic similarity among Coffea arabica L. accessions from Tanzania and to estimate levels of genetic similarities in C. arabica and diploid coffee species. The six ISSR primers used generated a total of 82 fragments and the dissimilarity values ranged
from 0.21 to 1. Mean dissimilarity values between provenances (0.56–0.85) were higher than within provenances (0.37–0.68).
Cluster analysis based on Nei’s genetic distances showed C. arabica provenances grouping based on geographical origin. Two major clusters were formed that constituted of provenances from Kilimanjaro
and Arusha in one sub-cluster; Tanga and Morogoro in the other; the second cluster had Mbeya provenances and diploid species,
respectively. The implication is that Mbeya provenances are different from the rest of Tanzanian C. arabica. A principal coordinate analysis (PCA), whose first three coordinates explained 43% of the variation, showed similar groupings
as in the cluster analysis. A separate cluster analysis of diploid species showed a distinct separation of the three species
used. ISSR data gave results similar to previous findings from random amplified polymorphic DNA(RAPD) analysis. The results
also confirm the limited diversity present in cultivated C. arabica in Tanzania 相似文献