Foliar Persistence and Residual Activity of Tebufenozide Against Spruce Budworm Larvae

A field study was conducted to investigate the persistence of tebufenozide in white spruce foliage. An aqueous suspension concentrate formulation, RH-5992 2F, was sprayed over single trees at three dosage rates, 35, 70 and 140 g of the active ingredient (AI), in 2·0 litre ha⁻¹, using ground application equipment. Foliage was collected at different intervals of time up to 64 days after treatment and tebufenozide residues were measured by high-performance liquid chromatography. Foliage was also fed to laboratory-reared 4th- and 6th-instar spruce budworm (Choristoneura fumiferana Clemens). The data indicated that tebufenozide residues in foliage declined with time according to first-order kinetics. The average rate-constant and half-life of disappearance (DT₅₀) were 0·0340 and 20·45 days, respectively. Larval mortality declined gradually, corresponding to the residues, but was still appreciable (49 to 70%) when the larvae were fed with foliage collected 64 days after treatment. The amount of foliage consumed by the larvae decreased when foliar residues of tebufenozide increased, thus indicating anti-feedant activity of the chemical. The LD₅₀ values for both instars were similar and averagedc.25 ng per insect, but the LD₉₀ values were significantly lower for 4th-instar than for 6th-instar, at 63·6 and 96·1 ng per insect respectively. This implies that, theoretically, at a foliar concentration of 1·0 μg tebufenozide g⁻¹ foliage (fresh wt), the spruce budworm larva needs to consume 65 to 100 mg of foliage in 10 days to cause mortality in about 90% of a population of the insect.     

Formulation selection,and investigation of azadirachtin-A persistence in some terrestrial and aquatic components of a forest environment

Five commercial formulations of azadirachtin-A (AZ-A) Margosan-O^®, Azatin-EC^®, Neem-EC^®, RH-9999 and Neemix^® 4.5, were investigated for their volatilization and washoff potential in laboratory studies. Prior to the investigation, RH-9999 (a wettable powder) was mixed with water to provide an end-use formulation containing 35·6 g AZ-A kg^-1, while the remaining four formulations were investigated without dilution. Volatilization and washoff of AZ-A occurred more from white spruce foliage than from wax-coated glass plates. Neem-EC provided the lowest amount of loss, whereas Margosan-O provided the highest. Physical properties and atomization behaviour of the five formulations indicated that Azatin-EC was highly viscous and caused phase separation in droplets collected on glass plates after atomization in a rotary atomizer. RH-9999, despite its low viscosity, caused phase separation in droplets because of the heterogeneity of the wettable powder formulation. Based on the minimum loss of AZ-A due to volatilization and washoff from spruce foliage, and on the minimum potential for phase separation in droplets after atomization in a rotary atomizer, Neem-EC was considered to be the most appropriate choice for use in field studies to investigate environmental persistence and fate of AZ-A in terrestrial and aquatic matrices of a forest ecosystem. The Neem-EC formulation was sprayed at 40 and 80 g AI ha^-1 over single spruce trees and on litter and soil plots selected in a mixed-wood boreal forest in Ontario, Canada. In addition, outdoor aquaria containing stream water and sediment were also fortified with the formulation at 400 and 800 g AI ha^-1. Persistence of AZ-A was evaluated using one-year-old spruce needles, current-year shoots, spruce bark, litter, soil, stream water and sediment. The duration of persistence varied from 3 to 6 days in terrestrial matrices, whereas it ranged from 8 to 13 days in water, and 2 to 3 days in sediment. The half-life (DT₅₀) values ranged from 10·7 h (for soil) to 71·6 h (for spruce bark) at the lower dosage rate, and from 18·8 h (for litter) to 76·2 h (for bark) at the higher dosage rate. The DT₅₀ value for stream water was about 35 h regardless of the dosage rate applied. The data indicated that AZ-A was appreciably labile and short-lived in different forestry matrices, with low DT₅₀ values. © 1997 SCI     

Anwendung von Isoproturon, allein und in Kombination mit anderen Wirkstoffen, im Nachauflauf-Winter (NA-W) und Nachauflauf-Frühjahr (NA-F) zu Winterweizen und Wintergerste

Use of isoproturon, alone and in combination with other compounds, post-emergence in winter and spring on winter wheat and winter barley Isoproturon, alone and combined with dinoseb acetate and bifenox, was applied post-emergence in winter and spring to winter barley and winter wheat. The experiment was on two sites (sandy and clay soils) and lasted for two years. Although after winter application the initial herbicide level in the soil was higher than after spring treatment, there were no differences at the end of the growing season. The DT₅₀ (time to 50% disappearance) value for isoproturon was between 12 and 33 days in both years for both soils and the DT₉₀ value varied from 34 to 68 days. Soil residues of isoproturon were not affected by the presence of the other compounds. Winter application gave better weed control and higher crop yields.     

A Field Bioassay Approach to Assess the Toxicity of Insecticide Residues on Soil to Collembola

Field bioassays were conducted to assess the toxicity of three insecticides, chlorpyrifos, cypermethrin and pirimicarb, to four species of springtails, Isotoma viridis, Isotomurus palustris, Folsomia candida (Collembola: Isotomidae) and Sminthurus viridis (Collembola: Sminthuridae). Spray residues on two soil types (a sandy clay loam and a sandy soil) were obtained in the field, in the presence and absence of a wheat crop canopy, after spray application by a commercial tractor-mounted sprayer. Collembola were then confined for 24-h periods on the sprayed soils in a constant laboratory environment at 1, 2, 3, 8 and 15 days after treatment. Residual insecticide toxicity was compared between species, insecticides, soils and exposure conditions (crop or no crop) using the age of residue at which median mortality occurred (DAT₅₀). Cypermethrin and pirimicarb residues were of low toxicity, causing less than 10% mortality, whereas residues of chloryprifos were toxic to all four species of Collembola on both soil types and in both exposure treatments. Interspecific differences in collembolan susceptibility to chlorpyrifos residues gave the ranking (from most to least susceptible) S. viridis>F. candida>Isotomurus palustris>Isotoma viridis. Residues on the sandy soil were more toxic than those on the sandy clay loam. These results are discussed in terms of how field bioassay approaches may be used to determine pesticide residual toxicity to microarthropods. We conclude that field bioassays offer a feasible method for evaluating the toxicity of pesticides and the persistence of toxic effects on Collembola. Advantages and disadvantages of this method are considered.     

Sorption and dissipation of aged metolachlor residues in eroded and rehabilitated soils

BACKGROUND: Sorption and dissipation of aged metolachlor were characterized in rehabilitated and eroded prairie soils using sequential batch slurry (conventional) and accelerated solvent extraction (ASE). RESULTS: In spite of an almost twofold difference in soil organic carbon (OC) content, S‐metolachlor sorption coefficients (K_d) and dissipation rates (DT₅₀) were the same in soils from different landscape positions within an eroded landform. Soil was moved within the landform to increase productivity. In areas receiving topsoil addition, S‐metolachlor K_d was higher and DT₅₀ was longer than in eroded areas. The efficiency of extraction was higher for ASE than for conventional extractions. No consistent aging effect on K_d was observed. Mineralization in 8 weeks accounted for < 10% of the applied metolachlor. CONCLUSION: The results of this laboratory study support a field dissipation study. Both showed that S‐metolachlor has the same retention and dissipation rate throughout an eroded landform, which was not expected owing to the large variability in soil properties, including OC concentrations. Altering soil properties by adding topsoil increased metolachlor sorption and persistence. The method of extraction (conventional versus ASE) affected calculated sorption coefficients and dissipation rates. In all cases, groundwater ubiquity scores (GUSs) categorized metolachlor as having intermediate mobility. Published 2012 by John Wiley & Sons, Ltd.     

Dissipation of tralkoxydim herbicide from wheat crop and soil under sub-tropical conditions

The persistence of tralkoxydim herbicide in wheat crop and in soil was evaluated under Indian sub-tropical field conditions at two application rates (400 g a.i ha ^?1 and 800 g a.i ha ^?1). At 400 g a.i ha ^?1, tralkoxydim persisted up to 28 days in soil but became non-detectable only after 45 days in the crop. However, at 800 g a.i ha ^?1, tralkoxydim residues persisted for 45 days in both soil and crop. The dissipation of the herbicide from both soil and crop appeared to occur in two phases at both rates of application. Each phase followed first-order kinetics. The values of DT₅₀ and DT₉₀ for both soil and crop are reported.     

Movement and persistence of imazaquin, oxyfluorfen, flurochloridone and terbacil in soil

The movement and persistence of imazaquin, oxyfluorfen, flurochloridone and terbacil residues were studied using a bioassay based on the shoot growth of Raphanus sativus L. var, radicula DC (radish) in a sandy loam soil under field conditions in 1992 and 1993. Imazaquin (0.3 kg a.i. ha^-1) was more mobile in the soil than the other three compounds. In 1992, under heavy rainfall conditions, the herbicide was distributed down to 60 cm in amounts of about 0.01 mg kg^-1. Conversely, in the dry year of 1993, the maximum depth of penetration was 20 cm and persistence in the surface soil layer (0-5 cm) was greater. After the first month in 1992, residues of terbacil were distributed in the 0-20 cm layer, and after 2, 3 and 5 months the herbicide had penetrated to a depth of 40 cm. In 1993, terbacil residues were distributed in the 0-10 cm layer only. In both years, residues of flurochloridone (1.0 kg a.i. ha^-1) were located mainly in the 0-5 cm layer, but there was some penetration to 10 cm. Oxy-fluorfen (1.2 kg a.i, ha^-1) showed least mobility in the soil profile. It was retained in the 0-5 cm layer throughout the experimental period in both. years.     

Spatial variability in the degradation rates of isoproturon and chlorotoluron in a clay soil

Thirty separate soil samples were taken from different locations at the Brimstone farm experimental site, Oxfordshire, UK. Incubations of isoproturon under standard conditions (15 °C; ?33 kPa soil water potential) indicated considerable variation in degradation rate in the soil, with the time to 50% loss (DT₅₀) varying from 6 to 30 days. These differences were confirmed in a second comparative experiment in which degradation rates were assessed in 11 samples of the same soil in two separate laboratories using an identical protocol. There was a significant negative linear relationship (r²= 0.746) between the DT₅₀ values and soil pH in this group of soils. In a third experiment, degradation rates of the related compound chlorotoluron were compared with those of isoproturon in 12 separate soil samples, six of which had been stored for several months, and six of which were freshly collected from the field. Degradation of both herbicides occurred more slowly in the stored samples than in the fresh samples but, in all of them, chlorotoluron degraded more slowly than isoproturon, and there was a highly significant linear relationship (r²=0.916) between the respective DT₅₀ values.     

Activity,adsorption, mobility and field persistence of sulfosulfuron in soil

Petri dish bioassays, based on root response of corn grown in soil or in perlite, were used to study the activity, adsorption, mobility and field persistence of sulfosulfuron in a silty clay loam and a sandy loam soil. Both bioassays indicated that activity of sulfosulfuron increased with increasing herbicide concentration, and to a slightly greater degree in sandy loam soil than in silty clay loam soil. More sulfosulfuron was adsorbed on the sandy loam (not biologically available) than on the silty clay loam soil. Consequently, slightly greater amounts of sulfosulfuron were leached through the silty clay loam than through the sandy loam soil. Biologically available sulfosulfuron was not detected at depths below 40 cm after application in sandy loam, but this was not the case for the silty clay loam soil. In 2002, all sulfosulfuron rates showed field persistence of less than 5 months. On the other hand, in 2003, biologically available sulfosulfuron was detected in the 0–10-cm soil depth 150 days after application. http://www.phytoparasitica.org posting May 6, 2004.     

Assessment of leaching potential of aldicarb and its metabolites using laboratory studies

The metabolites of pesticides can contaminate groundwater and pose a risk to human health when this water is used for drinking. This paper reports the results of a laboratory study on aldicarb and its main metabolites, aldicarb sulfone and aldicarb sulfoxide. Aldicarb and its metabolites showed K_oc values (6–31) which were lower than that of atrazine (55), indicating that they are very mobile in soil. They are less persistent than atrazine (DT₅₀ = 25 days), with DT₅₀ values from less than 1 day and up to 12 days. Aldicarb behaved as a non‐leacher, whereas its metabolites clearly showed the characteristics of leachers. Aged residue leaching experiments showed that aldicarb can occur at high concentrations in the leachate, together with its two metabolites. The leachate composition depends on the incubation time of the parent compound. Aldicarb and its metabolites can form various mixtures in groundwater on the basis of the time elapsing between the application of the insecticide and the first significant rainfall. This study confirms the characteristics of contaminants of aldicarb and especially its metabolites, as reported in the literature. © 2001 Society of Chemical Industry     

敌草胺在土壤中的吸附及其与持久性和生物有效性之间的关系

土壤吸附是农药在环境中归趋的关键支配因素,也是支配农药在环境中的持久性和生物有效性的重要因素之一。该文采用高效液相色谱法研究了除草剂敌草胺在不同性质土壤中的吸附、持久性和生物有效性以及吸附与土壤持久性、蚯蚓生物有效性之间的关系。结果表明,在供试浓度范围内,采用批量平衡技术测定的敌草胺土壤吸附等温线可用Freundlich模型表征（r>0.99）,土壤有机质含量（PPt50）在61.3-97.6 d之间;微生物对敌草胺在土壤中的持久性影响显著,微生物降解是敌草胺在土壤环境中降解的主要途径,灭菌处理后其在土壤中的半衰期延长了2.09~3.65倍。蚯蚓Eisenia foetida对敌草胺的吸收和生物积累也主要取决于土壤性质,特别是土壤的有机质含量水平（Pr=-0.885,Pr=-0.796,Pt50=94.210-3.535 Kf和BAF=0.264-0.014 Kf,表明吸附系数可用作模型参数来评价敌草胺在土壤中的持久性和生物有效性。     

低分子量有机酸对二氯喹啉酸在土壤中吸附-解吸的影响

采用高效液相色谱仪及批量平衡试验方法,研究了乙酸、苹果酸、酒石酸、草酸、丁二酸和柠檬酸6种低分子量有机酸对麻沙泥和第四纪红土红壤吸附-解吸二氯喹啉酸的影响。结果表明:低分子量有机酸可推迟二氯喹啉酸在土壤中的吸附平衡时间,其吸附动力学过程可用准二级动力学方程描述。Linear和Freundlich方程能较好地拟合二氯喹啉酸在供试两种土壤中的吸附等温线;二氯喹啉酸在麻沙泥中的吸附能力(lg Kf值)从大到小依次为苹果酸柠檬酸草酸=乙酸丁二酸酒石酸,在第四纪红土红壤中为苹果酸丁二酸乙酸草酸柠檬酸酒石酸;低分子量有机酸浓度对二氯喹啉酸解吸的影响因有机酸种类和供试土壤的不同而差异较大,6种供试有机酸均促进了第四纪红土红壤对二氯喹啉酸的解吸,且其解吸率均明显高于麻沙泥对二氯喹啉酸的解吸率,但在麻沙泥中呈现不同的影响模式。     

农田土壤入渗特性研究

以陕西杨凌一级阶地砂壤土和三级阶地粘壤土所选典型田块的双环入渗试验为基础,对不同土壤质地条件下的土壤入渗特性进行了研究。结果表明：两种土壤质地条件下描述其入渗过程的最优入渗公式为修正Kostiakov公式;土壤入渗试验合理的设计观测时间一级阶地砂壤土条件下不应小于90 min,三级阶地粘壤土条件下不应小于120 min;根据各入渗参数的最大相关距离,计算了典型田块入渗试验的合理测点数,一级阶地砂壤土和三级阶地粘壤土试验田块均为42个测点;可在一定程度上借助稳定入渗率f₀的变异特征描述土壤入渗性能和衡量土壤入渗能力,但同时还应考虑入渗参数k的空间变异特征。     

Toxicity and horizontal transfer of chlorantraniliprole against the Asian subterranean termite Coptotermes gestroi (Wasmann): effects of donor:recipient ratio, exposure duration and soil type

BACKGROUND: The effectiveness of chlorantraniliprole and other insecticides (bifenthrin, fipronil, indoxacarb, imidacloprid and chlorfenapyr) were tested against Coptotermes gestroi (Wasmann). Four experiments were conducted: a topical bioassay, a horizontal transfer study, an insecticide bioavailability test and a feeding bioassay. RESULTS: The topical bioassay showed that chlorantraniliprole was significantly less active to C. gestroi at 24 h post‐treatment compared with the other insecticides tested. Nevertheless, it is likely that a lesser amount of chlorantraniliprole was required to cause 50% mortality of C. gestroi at 7 and 14 days post‐treatment. The exposure duration and donor:recipient ratio affect the mortality of recipient termites. Mortality after exposure to chlorantraniliprole in sandy clay was significantly lower than in sand; however, by 14 days, > 90% of donor and recipient termites died in both substrates, irrespective of concentration. Fipronil and imidacloprid showed faster action, and high to moderate toxicity to C. gestroi. Termite workers also ceased to feed after exposure for 1 h to 50 mg kg^?1 chlorantraniliprole‐treated sandy clay. CONCLUSION: Chlorantraniliprole demonstrated delayed toxicity at the lowest label rate (50 mg kg^?1) in sandy clay. Its slow action will enable greater transfer of toxicant between nestmates, while feeding cessation will promote greater social interaction between healthy and exposed termites. Copyright © 2011 Society of Chemical Industry     

滴灌高频施肥条件下玉米适宜灌水量的试验研究

通过大田试验,在高频施肥条件下设置了低水(H1,0.56K_cET_0),中水(H2,0.8K_cET_0),高水(H3,1.04 K_cET_0)3个不同灌水量,研究风沙土滴灌灌水量对玉米生长、干物质积累和产量的影响。结果表明:H1处理土壤含水率长期低于60%的田间持水率,H2和H3处理土壤水分能长期保持在田间持水率的60%以上;灌水量对玉米生长有显著影响,表现为H2处理玉米株高在灌浆期和完熟期显著高于H1和H3处理,其中灌浆期比H1和H3处理分别高13.8%和10.8%,完熟期分别高12.9%和10.7%;H3处理的茎粗在拔节期～完熟期显著高于H1处理,拔节期H3处理的茎粗最大,为28.9 mm,比H1处理粗8.5%;H2处理的叶面积指数(LAI)分别比H1和H3处理高22.6%和16.7%;H2处理的叶绿素含量(SPAD值)在穗期和灌浆期显著高于H1和H3处理,其中穗期分别高13.8%和10.8%,灌浆期分别高12.9%和10.7%;地上部干物质量H2处理最大,达303.5 g,比H1和H3处理分别增加了15.4%和3.3%;灌水量对各单项产量构成性状的影响不显著,但H2处理穗重最重,达382.9 g,比H1和H3处理分别重30.8%和4.2%,产量和水分利用效率(WUE)从大到小依次为H2、H3、H1,H2处理的产量和WUE分别为12.3 t·hm~(-2)和2.16 kg·m~(-3)。在风沙土地区,低水处理会使玉米前期缺水,中、高水处理能为玉米提供适宜的水分条件,但高水处理降低了肥效。综上,风沙土地区玉米滴灌高频施肥条件下推荐灌溉水量为0.8K_cET_0。     

Aerobic soil metabolism of metsulfuron-methyl

A laboratory study was conducted to determine the degradation rates and identify major metabolites of the herbicide metsulfuron-methyl in sterile and non-sterile aerobic soils in the dark at 20°C. Both [phenyl-U-¹⁴C]- and [triazine-2-¹⁴C]metsulfuron-methyl were used. The soil was treated with [¹⁴C]metsulfuron-methyl (0.1 mg kg⁻¹) and incubated in flow-through systems for one year. The degradation rate constants, DT₅₀, and DT₉₀ were obtained based on the first-order and biphasic models. The DT₅₀ (time required for 50% of applied chemical to degrade) for metsulfuron-methyl, estimated using a biphasic model, was approximately 10 days (9–11 days, 95% confidence limits) in the non-sterile soil and 20 days (12–32 days, 95% confidence limits) in the sterile soil. One-year cumulative carbon dioxide accounted for approximately 48% and 23% of the applied radioactivity in the [phenyl-U-¹⁴C] and [triazine-2-¹⁴C]metsulfuron-methyl systems, respectively. Seven metabolites were identified by HPLC or LC/MS with synthetic standards. The degradation pathways included O-demethylation, cleavage of the sulfonylurea bridge, and triazine ring opening. The triazine ring-opened products were methyl 2-[[[[[[[(acetylamino)carbohyl]amino]carbonyl]amino] carbonyl]-amino]sulfonyl]benzoate in the sterile soil and methyl 2-[[[[[amino[(aminocarbonyl)imino]methyl] amino]carbonyl]amino]sulfonyl]benzoate in the non-sterile soil, indicating that different pathways were operable. © 1999 Society of Chemical Industry     

Soil persistence studies with bromoxynil, propanil and [14C]dicamba in herbicidal mixtures

The persistence of bromoxynil (3,5-dibromo-4-hydroxybenzonitrile), [¹⁴C]dicamba (3,6-dichloro-2-methoxybenzoic-7-¹⁴C acid) and propanil [N-(3,4-dichlorophenyl)propionamide] at rates equivalent to 1 kg ha^?1, were studied under laboratory conditions in a clay loam, a heavy clay and a sandy loam at 85% of field capacity and at 20±1°C, both singly and in the presence of herbicides normally applied with these chemicals as tank-mix or split-mix components. The degradation of bromoxynil was rapid with over 90% breakdown occurring within a week in the heavy clay and sandy-loam soils, while in the clay-loam approximately 80% of the bromoxynil had broken down after 7 days. In all three soils degradation was unaffected by the presence of asulam, diclofop-methyl, flamprop-methyl, MCPA, metribuzin or propanil. Propanil underwent rapid degradation in all soil treatments, with over 95% of the applied propanil being dissipated within 7 days. There were no noticeable effects on propanil degradation resulting from applications of asulam, barban, bromoxynil, dicamba, MCPA, MCPB, metribuzin or 2,4-D. The breakdown of [¹⁴C]dicamba in a particular soil was unaffected by being applied alone or in the presence of diclofop-methyl, flampropmethyl, MCPA, metribuzin, propanil or 2,4-D. The times for 50% of the applied dicamba to be degraded were approximately 16 days in both the clay loam and sandy loam, and about 50 days in the heavy clay.     

Factors affecting the distribution of pesticide drenches in soil using the fungicide metazoxolon as a model

The distribution resulting from the drenching of soil with a suspension concentrate of [¹⁴C]metazoxolon was studied in the laboratory and the field. Penetration of soil columns was increased by (a) increasing the drench volume from 1 to 7.8 litres m^?2, (b) changing the original soil moisture content from air-dry to field-capacity, and (c) including 1 % of ‘Renex 30’ surfactant in the drench. Penetration was greatest in soils containing large pores and was reduced when aggregates were broken down by sieving. Leaching the column with 1.56 cm of ‘rain’, 15 h after treatment, did not increase the penetration by metazoxolon. In all experiments, the maximum concentration of metazoxolon occurred in the top 2 cm of soil. Equivalent effects were found when metazoxolon was applied to a poorly-structured sandy clay loam in the field.     

Retention and degradation of metribuzin in sandy loam and clay soils of Lebanon

The retention and degradation of metribuzin herbicide were studied under two environmental conditions. Field studies were carried out on two soils, a sandy loam soil (soil A) and a clay soil (soil B). Metribuzin was applied with a jet sprayer at 1060 g a.i. ha^?1 and 1960 g a.i. ha^?1 on soils A and B respectively. Reconstituted soil columns were used to study the herbicide movement and metabolism in the two soils. Analyses of metribuzin and its metabolites were carried out using standardized methods. The results indicated a very weak capacity of adsorption of metribuzin in the two soils, and the weak adsorbed fraction is easily desorbed. Degradation and mobility of metribuzin in the field and laboratory soil columns were very intense and rapid. Soil A favoured reductive deamination whereas soil B favoured oxidative desulphuration and the respective metabolites deaminometribuzin and diketometribuzin yield the same product deaminodiketometribuzin. Both leaching by rainfall and degradation were important in the disappearance of metribuzin from the soils.     

Persistence of GR7 and Striga germination stimulant(s) from Euphorbia aegyptiaca Boiss. in soils and in solutions

The activity of GR7 and Striga germination stimulant(s) from Euphorbia aegyptiaca Boiss. showed adequate persistence (6–8 days) in acidic soils (pH 5·0–6·3), but residual activity was short (1–3 days) in alkaline soils. The compounds tended to lose activity at a faster rate in the alkaline clay Gezira soil (pH 7·8), than in its sandy equivalent (pH 8·1). In solution, pH had no influence on initial activity, but residual action was reduced more rapidly by alkalinity and high temperature. However, the rate of loss of activity in solution was slower than in soils.