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To identify the potential of green leafy vegetables (GLV) as antioxidants, methanolic extracts of Amaranthus sp., Centella asiatica, Murraya koenigii and Trigonella foenum graecum were studied for their antioxidant activity in different systems at multiple concentrations. Total antioxidant activity assessed
by phosphomolybdenum method, free radical scavenging activity by 1,1-diphenly-2-picryl hydrazyl (DPPH), reducing power and
ferrous ion chelating activity were determined. The GLV were analyzed for ascorbic acid, total and β-carotene and total polyphenol
contents. The ascorbic acid, total carotene, β-carotene and total phenolic content (tannic acid equivalents) of the GLV ranged
between 15.18–101.36, 34.78–64.51, 4.23–8.84 and 150.0–387.50 mg/100 g GLV, respectively. The extracts were found to have
significantly different levels of antioxidant activities in the systems tested. The total antioxidant activity was highest
in Murraya koenigii (2,691.78 μmol of ascorbic acid/g sample) and least in Centella asiatica (623.78 μmol of ascorbic acid/g sample). The extract concentration causing 50% inhibition of DPPH (IC50) was determined (M. koenigii < C.asiatica < Amaranthus sp. < T. graecum). The maximum DPPH scavenging activity and reducing power was exhibited by Murraya koenigii. Multiple regression analysis showed that the relationship of total antioxidant activity, free radical scavenging activity,
and reducing power with polyphenol and total and β-carotene was highly significant.
Paper awarded the Young Scientist Award in Experimental Nutrition at the Young Scientist Award Session of the 39th Annual
Meet of the Nutrition Society of India, Hyderabad, India on 15–16th Dec 2007. 相似文献
3.
Paśko P Zagrodzki P Bartoń H Chłopicka J Gorinstein S 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):333-338
The effect of Chenopodium quinoa seeds on lipid profile, glucose level, protein metabolism and selected essential elements (Na, K, Ca, Mg) level was determined
in high—fructose fed male Wistar rats. Fructose decreased significantly LDL [42%, p < 0.01] and activity of alkaline phosphatase [20%, p < 0.05], and increased triglycerides level [86%, p < 0.01]. The analysis of blood of rats fed quinoa indicated, that these seeds effectively reduced serum total cholesterol
[26%, p < 0.05], LDL [57%, p < 0.008] and triglycerides [11%, p < 0.05] when compared to the control group. Quinoa seeds also significantly reduced the level of glucose [10%, p < 0.01] and plasma total protein level [16%, p < 0.001]. Fructose significantly decreased HDL [15%, p < 0.05] level in control group but when the quinoa seeds were added into the diet the decrease of HDL level was inhibited.
Quinoa seeds did not prevent any adverse effect of increasing triglyceride level caused by fructose. It was shown in this
study that quinoa seeds can reduce most of the adverse effects exerted by fructose on lipid profile and glucose level. 相似文献
4.
Yong-Seo Park Mi-Kyung Lee Buk-Gu Heo Kyung-Sik Ham Seong-Gook Kang Ja-Yong Cho Shela Gorinstein 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(2):186-191
This study was conducted in order to compare the nutrient and chemical contents of two Korean teas: traditional Chungtaejeon
tea (CTJ) with that of green tea (GT). Main bioactive compounds and the antioxidant activities using four radical scavenging
assays (ABTS, CUPRAC, FRAP and DPPH) in methanol and acetone extracts of both teas were determined. It was found that the
contents of vitamin C, amino acids and total nitrogen in CTJ were lower than that of GT (p < 0.05). Caffeine, reducing sugar and chlorophyll contents in CTJ were similar to GT. Catechin (C), epicatechin (EC), and
epigallocatechin (EGC) contents were lower in CTJ than in green tea (p < 0.05), but gallocatechin (GC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) and catechin gallate (CG) showed
no significant differences between CTJ and GT (p ≥ 0.05). The contents of polyphenols, flavonoids, flavanols and tannins in
CTJ methanol were 229.30 ± 11.3 mg GAE/g dry weight (DW), 15.24 ± 0.8 mg CE/g DW, 109.10 ± 5.1 mg CE/g DW and 25.68 ± 1.2
mg CE/g DW, respectively, and significantly higher than in acetone extracts (p < 0.05). Flavonoids (quercetin and kaempferol) were higher in GT than in CTJ and myricetin was higher in CTJ (p < 0.05). Threonine and aspartic acid was lower, and glutamic acid was higher in CTJ compared with GT (p < 0.05). Free amino acid content in CTJ and GT showed no significant difference. Potassium and magnesium in CTJ were lower
compared to GT, but no significant difference was found for iron, manganese and calcium. Also, the level of the antioxidant
activity by all four used assays was significantly higher in CTJ and in methanol was higher than in acetone extracts (p < 0.05). In conclusion, traditional fermented Korean tea Chungtaejeon contains high quantities of bioactive compounds and
possesses high antioxidant activity. The contents of the bioactive compounds and the levels of antioxidant activities are
significantly higher in methanol than in acetone extracts. 3-D fluorescence and FTIR- spectroscopy showed slight differences
between the two investigated tea samples and can be used as additional tools for identification of polyphenols. Both studied
teas can be recommended as a source of bioactive compounds. 相似文献
5.
Jarinyaporn Naowaboot Patchareewan Pannangpetch Veerapol Kukongviriyapan Bunkerd Kongyingyoes Upa kukongviriyapan 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(2):116-121
In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration
of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced
diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the
effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 ± 0.33
and 3.76 ± 0.18 μM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 ± 0.45 and 7.50 ± 0.46 μM, respectively). Hemoglobin A1C, a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group
(6.78 ± 0.30%) in comparison to untreated group (9.02 ± 0.30%). The IC50 of in vitro antiglycation and free radical scavenging activities of MA were 16.4 ± 5.6 μg/ml and 61.7 ± 2.1 μg/ml, respectively. These
findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic
diabetic rats, which may be beneficial as food supplement for diabetics. 相似文献
6.
Rinelli S Spadafranca A Fiorillo G Cocucci M Bertoli S Battezzati A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):100-104
We hypothesized that fruit ingestion provides measurable amounts of salicylic acid (SA) and produces different metabolic and
inflammatory responses compared to mere fruit sugars. In a randomized-crossover study, 26 healthy subjects received a peach
shake meal (PSM) (SA: 0,06 ± 0,001 mg/100 g) and a mixed sugar meal (MSM), consisting in an aqueous solution with the same
sugars found in the peach shake. In order to control for the SA contribution from meals in the previous day, 16 subjects (Group
1) abstained from fruits and vegetables consumption the evening before trials, and 10 subjects (Group 2) maintained their
usual diet. Circulating SA, glucose, insulin, free fatty acids, and interleukin-6 were determined. Basal SA was lower in Group
1 than in Group 2 (0.09 ± 0.02 vs. 0.30 ± 0.03 μmol/l, p < 0.001), peaked at 90 min in both groups (0.18 ± 0.01 vs. 0.38 ± 0.02 μmol/l, p < 0.01) and remained above baseline (p < 0.05) up to 3 h. Glycemia increased less after PSM at 15 min (p < 0.01) with a lower average glucose excursion (p < 0.05). Insulin peaked at 45 min with both meals but decreased less rapidly with PSM. Free fatty acids decreased more (p < 0.01), and interleukin-6 increased less (p < 0.05) with PSM. Dietary fruit intake increases the concentration of SA in vivo, and provides non-nutrients capable to modulate the inflammatory and metabolic responses to carbohydrates. 相似文献
7.
Bioavailability of Phenolic Antioxidants Associated with Dietary Fiber: Plasma Antioxidant Capacity After Acute and Long-Term Intake in Humans 总被引:1,自引:1,他引:0
Jara Pérez-Jiménez José Serrano Maria Tabernero Sara Arranz M. Elena Díaz-Rubio Luis García-Diz Isabel Goñi Fulgencio Saura-Calixto 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(2):102-107
Most studies on bioavailability of phenolic antioxidants are focused in foods and beverages in which they may be easily released
from the food matrix, reaching a peak in plasma antioxidant capacity 1–2 h after the intake. However, plant foods contain
significant amounts of polyphenols associated with dietary fiber. The aim of the present work was to seek the bioavailability
of total phenolic antioxidants associated with dietary fiber by measuring plasma antioxidant capacity in human volunteers.
An acute intake of 15 g of a dietary fiber rich in associated phenolic antioxidants in healthy volunteers (n = 10) increased antioxidant capacity of plasma in relation to a control group (n = 4), becoming significant 8 h after the intake. This shows that phenolic antioxidants associated with dietary fiber are
at least partially bioavailable in humans, although dietary fiber appears to delay their absorption. No significant changes
were observed after long-term intake (16 weeks, 34 subjects). 相似文献
8.
Renata Mikstacka Agnes M. Rimando Ewa Ignatowicz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):57-63
There is evidence that a diet rich in fruit and vegetables may reduce the risk of cancer and other degenerative diseases.
However, potential health impact of bioactive phytochemicals is limited by their low amount and relatively poor bioavailability.
It has been suggested that the health benefits associated with fruit and red wine consumption could be due to the whole antioxidant
pool of the diet microcomponents. In this study, the antioxidant activities of trans-resveratrol, pterostilbene and quercetin, and the effect of their combination were investigated in human erythrocytes in vitro. H2O2-induced lipid peroxidation was assessed by measuring the amount of thiobarbituric acid reactive species. Quercetin and pterostilbene
protected erythrocyte membranes against lipid peroxidation (IC50 values = 64 ± 8.7 μM and 44.5 ± 7.8 μM, respectively). Resveratrol was significantly less effective. However, the three compounds
protected the erythocytes against hemolysis and GSH (reduced glutathione) depletion to the same extent. Combinations consisting
of two compounds (molar ratio 1:1) influenced lipid peroxidation in a concentration–dependent manner. At lower concentrations,
resveratrol with quercetin or pterostilbene inhibited synergistically the oxidative injury of membrane lipids At higher concentrations,
an additive effect was observed. These protective effects may partially explain the health benefit of these bioactive microcomponents
when together in the diet. 相似文献
9.
Alison Gegios Rachel Amthor Busie Maziya-Dixon Chedozie Egesi Sally Mallowa Rhoda Nungo Simon Gichuki Ada Mbanaso Mark J. Manary 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):64-70
Cassava contains little zinc, iron, and β-carotene, yet it is the primary staple crop of over 250 million Africans. This study
used a 24-hour dietary recall to test the hypothesis that among healthy children aged 2–5 years in Nigeria and Kenya, cassava’s
contribution to the childrens’ daily diets is inversely related to intakes of zinc, iron, and vitamin A. Dietary and demographic
data and anthropometric measurements were collected from 449 Kenyan and 793 Nigerian children. Among Kenyan children 89% derived
at least 25% of their dietary energy from cassava, while among the Nigerian children 31% derived at least 25% of energy from
cassava. Spearman’s correlation coefficient between the fraction of dietary energy obtained from cassava and vitamin A intake
was r = −0.15, P < 0.0001, zinc intake was r = −0.11, P < 0.0001 and iron intake was r = −0.36, P < 0.0001. In Kenya, 59% of children consumed adequate vitamin A, 22% iron, and 31% zinc. In Nigeria, 17% of children had
adequate intake of vitamin A, 57% iron, and 41% zinc. Consumption of cassava is a risk factor for inadequate vitamin A, zinc
and/or iron intake. 相似文献
10.
Antioxidant activity of pressurized low polarity water (PLPW) extracts of cow cockle seed and extraction residues were determined
using DPPH, ABTS, and FRAP assays. The effect of extraction conditions (temperature (125, 150 and 175 °C) and time) on the
antioxidant activity and the relationship amongst the antioxidant activity and extract composition (total phenolics and saponin
content) were determined. The antioxidant activity of PLPW extracts increased with extraction temperature. Increasing activity
with time was also observed at 175 °C. PLPW extraction residues had the highest activity suggesting antioxidant compounds
were not completely extracted by PLPW. Antioxidant activity correlated well with total phenolics content of samples (R
2 ≥ 0.94), however no correlation was observed with the saponin content. A strong correlation was observed between the antioxidant
activity values obtained using different methods (R
2 ≥ 0.94). These results point to the potential of PLPW extraction as a method to modify the activity of biological materials
for the production of customized extracts. 相似文献
11.
Vaughn N Rizzo A Doane D Beverly JL Gonzalez de Mejia E 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(1):41-46
Some studies suggest that increased consumption of soy protein hydrolysates may cause body weight loss but the mechanism of
action is unknown. The objective of this investigation was to determine whether intracerebroventricular (i.c.v.) infusion
of soy protein hydrolysates decrease food intake and body weight. Adult male Sprague Dawley rats (n = 24) received i.c.v. injections of soy hydrolysate I (SH I) or soy hydrolysate II (SH II) three times weekly for 2 weeks.
Krebs solution and leptin were used as negative and positive controls respectively. SH I (6.5–20 kDa with a strong band at
14 kDa) was produced by hydrolysis with alcalase, and SH II (∼2 kDa) was obtained by hydrolysis and ultrafiltration. Leptin
successfully reduced body weight (−1.60 g) 24 h (p = 0.0093) after the third injection. SH I caused significant (p = 0.0009) decreases in body weight (−1.70 g) 24 h after the third injection but not after 48 h. SH II showed a tendency to
prevent body weight gain but this effect was short of statistical significance (p < 0.40). Food intake was not affected by any of the soy hydrolysate treatments but leptin injection did cause significant
decreases in food intake (p < 0.05). Data suggest that soy alcalase hydrolysate can decrease, in the short term, the rate of body weight gain independently
of food consumption. This preliminary data show that soy peptides may play a role on body weight regulation, possibly by increasing
energy utilization. 相似文献
12.
Dias MI Barros L Sousa MJ Ferreira IC 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):181-186
Coriander is commonly used for medicinal purposes, food applications, cosmetics and perfumes. Herein, the production of antioxidants
in vegetative parts (leaves and stems) of in vivo and in vitro grown samples was compared. In vitro samples were clone A- with notorious purple pigmentation in stems and leaves and clone B- green. Seeds were also studied
as they are used to obtain in vivo and in vitro vegetative parts. Lipophilic (tocopherols, carotenoids and chlorophylls) and hydrophilic (sugars, ascorbic acid, phenolics,
flavonols and anthocyanins) compounds were quantified. The antioxidant activity was evaluated by radical scavenging activity,
reducing power and lipid peroxidation inhibition. The in vivo sample showed the highest antioxidant activity mainly due to its highest levels of hydrophilic compounds. Otherwise, in vitro samples, mainly clone A, gave the highest concentration in lipophilic compounds but a different profile when compared to
the in vivo sample. Clones A and B revealed a lack of β-carotene, β- and δ-tocopherols, a decrease in α-tocopherol, and an increase in
γ-tocopherol and clorophylls in comparison to the in vivo sample. In vitro culture might be useful to explore the plants potentialities for industrial applications, controlling environmental conditions
to produce higher amounts of some bioactive products. 相似文献
13.
Peter Deriemaeker Dirk Aerenhouts Marcel Hebbelinck Peter Clarys 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):77-82
A first objective of the present study was to estimate the acid-base balance of the food intake in vegetarians and non-vegetarians.
A second objective was to evaluate if additional input of specific food items on the existing potential renal acid load (PRAL)
list was necessary for the comparison of the two dietary patterns. Thirty vegetarians between the age of 18 and 30 years were
matched for sex, age and BMI with 30 non-vegetarians. Based on the 3-days food diaries the acid-base status of the food intake
was estimated using the PRAL method. Mean PRAL values as estimated with the standard table yielded an alkaline load of -5,4 ± 14,4 mEq/d
in the vegetarians compared to an acid load of 10,3 ± 14,4 mEq/d in the non-vegetarians (p < 0,001). Mean PRAL values as estimated with the extended table yielded an alkaline load of -10,9 ± 19,7 mEq/d in the vegetarians
compared to an acid load of 13,8 ± 17,1 mEq/d for the non-vegetarians (p < 0,001). The findings of this study indicate that vegetarian food intake produces more alkaline outcomes compared to non-vegetarian
diets. The use of the standard PRAL table was sufficient for discrimination between the two diets. 相似文献
14.
Brandsch C Kappis D Weisse K Stangl GI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):410-416
Lupin protein is capable of reducing plasma lipids in hypercholesterolemic man and animals. Whether lipid-lowering properties
of lupin protein will be influenced by thermal treatment or by other nutrients has not been elucidated. In a two-factorial
study, rats were fed hypercholesterolemic diets based on high amounts of carbohydrates (HC) or fat (HF), which contained either
(20.4% of energy) untreated or thermally treated lupin protein (steam: 120 °C, 30 min) or casein as control protein. Lupin
protein lowered plasma lipid concentrations in rats fed the HF diet but not in those fed the HC diet (P < 0.05). Among rats fed the HF diet, plasma and VLDL triglyceride concentrations were lower in rats fed thermally treated
(−46% and −44%, P < 0.05) and untreated lupin protein (−47% and −46%, P < 0.05) than in those fed casein; whereas liver triglycerides were reduced only in rats fed untreated lupin protein (P < 0.05). Compared to casein, untreated lupin protein had slightly stronger cholesterol-lowering effects in plasma, LDL and
HDL (−34%, −37%, −35%; P < 0.05) than thermally treated lupin protein (−23%, −29%, −31%, P < 0.10). In conclusion, the lipid-lowering effect of lupin protein strongly depends on composition of the basal diet, and
thermal treatment is accompanied by a slight reduction of its hypocholesterolemic properties. 相似文献
15.
Jimenez M Guzman AP Azuara E Garcia O Mendoza MR Beristain CI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):57-63
Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional
medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude,
aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high
antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing
power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of
vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential
(561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal,
trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants. 相似文献
16.
Miranda-Velasquez L Oranday-Cardenas A Lozano-Garza H Rivas-Morales C Chamorro-Cevallos G Cruz-Vega DE 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):392-395
The aim of this study was to determine the hypocholesterolemic activity of Cnidoscolus chayamansa. In an in vivo model, high-cholesterol diet administered to mice Balb/c induced hypercholesterolemia. Three extracts from Cnidoscolus chayamansa (ethanol, methanol and an aqueous extract) were tested on hypercholesterolemic mice. Active extracts were assessed against
the in vitro inhibitory activity of the same three extracts on the HMG-CoA reductase enzyme by using Vero cells. The specific chemical
groups present in the phytochemical extracts were also determined. Only the aqueous extract (at either doses employed) showed
a significant cholesterol reduction (27.9 and 31.1%, for 50 and 100 mg kg−1, respectively P < 0.01). The extract did not inhibit the HMG-CoA reductase enzyme, suggesting that its compounds act at another level in
cholesterol metabolism. Reactions to secondary metabolites indicate the presence of alkaloids in the aqueous and ethanol extracts
and phenol hydroxyls in the ethanol and methanol extracts. 相似文献
17.
Effect of Green Tea and Vitamin E Combination in Isoproterenol Induced Myocardial Infarction in Rats
Aman Upaganlawar Chintan Gandhi Ramchandran Balaraman 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(1):75-80
The present study was aimed to investigate the combined effects of green tea and vitamin E on heart weight, body weight, serum
marker enzymes, lipid peroxidation, endogenous antioxidants and membrane bound ATPases in isoproterenol (ISO)-induced myocardial
infarction in rats. Adult male albino rats, treated with ISO (200 mg/kg, s.c.) for 2 days at an interval of 24 h caused a
significant (P < 0.05) elevation of heart weight, serum marker enzymes, lipid peroxidation and Ca+2 ATPase level whereas there was a significant (P < 0.05) decrease in body weight, endogenous antioxidants, Na+/ K+ ATPase and Mg+2 ATPase levels. Administration of green tea (100 mg/kg/day, p.o.) and vitamin E (100 mg/kg/day, p.o.) together for 30 consecutive
days and challenged with ISO on the day 29th and 30th, showed a significant (P < 0.05) decrease in heart weight, serum marker enzymes, lipid peroxidation, Ca+2 ATPase and a significant increase in the body weight, endogenous antioxidants, Na+/K+ ATPase and Mg+2 ATPase when compared with ISO treated group and green tea or vitamin E alone treated groups. These findings indicate the
synergistic protective effect of green tea and vitamin E during ISO induced myocardial infarction in rats. 相似文献
18.
Julio Beltrame Daleprane Tatiana Silveira Feijó Gilson Teles Boaventura 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(1):1-5
The aim of this study was to evaluate the protein quality of organic and genetically modified soy by feeding specific diets
to rats. Three groups of Wistar rats (n = 10) were used, and each group was named according to the food that they ate. There was an organic soy group (OG), a genetically
modified soy group (GG), and a control group (CG). All animals received water and diet ad libitum for 455 days. At the end of this period, the weight of the GG group was the same as that of the OG, and both were higher
than CG. Protein intake was similar for the OG and GG, which were significantly lower (p < 0.0005) than the CG. The growth rate (GR) of the rats, albumin levels, and total levels of serum protein were comparable
for all groups. Hematocrit (p < 0.04) and hemoglobin (p < 0.03) for the OG and GG were less than the CG. Although the OG and GG demonstrated reduced hematocrit and hemoglobin, both
types of soy were utilized in a way similar to casein. This result suggests that the protein quality of soy is parallel to
the standard protein casein in terms of growth promotion but not hematological indicators. 相似文献
19.
Laddawan Senggunprai Veerapol Kukongviriyapan Auemduan Prawan Upa Kukongviriyapan 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):403-409
Several vegetables have been shown to possess cytoprotective and antioxidant effects with various mechanisms of action. The
aim of this study was to determine the antioxidant effects and mechanism underlying of Syzygium gratum, a dietary and herbal plant commonly found in the Southeast Asia. Additionally, its effects on the induction of endogenous
antioxidant defensive system were also investigated. Results showed that the leaf extract possessed an exceptionally strong
antioxidant and intracellular oxygen radical scavenging activity in both aqueous and ethanolic extracts. The plant aqueous
extract was further studied in C57BL/6J mice to evaluate its effects in vivo. The extract was well tolerated by the animals throughout the 30 days of study. The cytoprotective enzyme, heme oxygenase
(HO-1) activity was significantly increased in the high dose-treated animals (1 g/kg/day). Consistent with the enzymatic activity,
the expression of HO-1 mRNA tended to increase in those mice. There was no significant increase in hepatic γ-glutamylcysteine
ligase (γ-GCL) activity, glutathione levels and GCL mRNA expression. Taken together, this study provides evidence that S. gratum exhibits potent direct antioxidant properties and can induce cytoprotective enzyme in vivo. Consumption of S. gratum may provide a health benefit against oxidative stress and other related disorders. 相似文献
20.
Nevcihan Gursoy Bektas Tepe 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(2):135-140
This study was designed to examine the in vitro antimicrobial and antioxidant activities and the amount of total phenolics of the methanol extracts of Ballota rotundifolia L. and Teucrium chamaedrys C. Koch. In the case of antimicrobial activity tests, polar sub-fractions of the methanol extracts of plant species exhibited
weakest antimicrobial activity when compared with the non-polar ones. While, non-polar sub-fraction of B. rotundifolia showed moderate activity against A. lwoffii, C. perfringens and the yeasts, T. chamaedrys performed excellent activity pattern against all of the tested microorganisms. The sub-fractions were also screened for their
possible antioxidant activities by two complementary tests, namely DPPH free radical-scavenging and β-carotene/linoleic acid assays. Non-polar extracts of the plant species remained inactive in both test systems. On the other
hand polar extracts showed remarkable antioxidant activities. In DPPH system, free radical scavenging effect of T. chamaedrys was measured as 18.00 ± 1.42 μg.mg−1. It is extremely important to point out that, polar sub-fraction of T. chamaedrys is found as effective as the positive control BHT. Non-polar sub-fraction of T. chamaedrys found to have the highest total phenolic amount (97.12 ± 1.28 μg/mg). Results obtained from this experiment confirm the relationship
between the amount of phenolics and biological activities. 相似文献