基于主动冠层光谱仪的莴苣生物量及氮素营养状况估测

[目的]研究冠层光谱技术在蔬菜氮素营养诊断中应用的可行性和提高其准确性的方法,为推进蔬菜氮素营养管理与施肥推荐提供快速无损检测技术.[方法]以茎菜类蔬菜—莴苣(Lactuca sativa L.)为研究对象进行田间试验.设置5个化肥年施用梯度:0、108、162、216、270kg/hm2,在莴苣幼苗期、莲座期、茎形成...     

The effect of chloride and sulphate salts on the nitrate content in lettuce plants (Lactuca sativa L.)

The effect of chloride and sulphate on the nitrate accumulated in lettuce plants (Lactuca sativa L., cv. deci minor) under low light conditions was examined. The plants were grown on a complete nutrient solution for circa 45 days and then chloride was substituted for the nitrate in the nutrient solution. In another experiment, sulphate was substituted for the nitrate in the nutrient solution. Approximately 5 days later a reduction in growth occurred. The nitrate in the plants was not completely exhausted and was very slowly available for reduction both in the leaf blades and midribs. It was immaterial whether sulphate or chloride was substituted for nitrate in the nutrient solution. Chloride was taken up from the solution but sulphate was not. In plants in both treatments, sugars accumulated concomitantly with the decrease in nitrate content.

The actual nitrate reductase activity in the leaf blades decreased several days after the chloride and sulphate treatments began and was negligible on day 12. Nevertheless, the potential nitrate reductase activity of the treated plants was still 50% of the control.

It is suggested that the availability of nitrate from the storage pool is the limiting process for nitrate reductase activity, and is independent of the metabolic demand. For commercial growers it may be of advantage to use hydroponics in which the nitrate can be removed easily several days before harvest, to reduce high nitrate contents in vegetables, particularly under low light conditions.     

Iodine application affects nitrogen-use efficiency of lettuce plants (Lactuca sativa L.)

Abstract

The main objective of this work was to determine whether nitrogen-use efficiency was affected by the application of different forms (iodide vs iodate) and dosages (20, 40 and 80 µM) of iodine, to ascertain the influence of this trace element in a biofortification programme in lettuce plants. The parameters analysed were root and shoot biomass, nitrate concentration, and organic and total nitrogen as well as those defining nitrogen-use efficiency in plants: total nitrogen content, total nitrogen accumulation, nitrogen-uptake efficiency and nitrogen-utilization efficiency. In addition to decreasing shoot biomass, iodide treatments reduced leaf levels of nitrates, organic nitrogen, and total nitrogen content. Iodate treatments did not affect the concentration of nitrogen in its different forms. The application of iodide caused total nitrogen accumulation and nitrogen-uptake efficiency to decrease, iodate application improved the latter. Both iodide and iodate applications significantly improved nitrogen-utilization efficiency in comparison to the control. The results obtained show that iodate application rates of 40 µM or lower significantly improved all nitrogen parameters analysed, making it possible to increase lettuce productivity and quality.     

Impact of liming on utilization of 59Fe‐chelates by lettuce (Lactuca sativa L.)

A pot experiment was conducted to determine the utilization of iron (Fe) by lettuce (Lactuca sativa L. cv. Australian gelber). Iron was applied as ⁵⁹Fe in inorganic and chelated form, particularly biodegradable chelate, ⁵⁹Fe‐EDDS. Two stereoisomeric forms of ethylenediaminedisuccinate: [S,S]‐EDDS and a mixture of EDDS containing 25% [S,S]‐EDDS, 25% [R,R]‐EDDS, and 50% [S/R]/[R/S]‐EDDS, ethylenediaminetetraaceticacid (EDTA) and ethylenediimino bis(2‐hydroxyphenyl)acetic acid (EDDHA) were used as ligands. Lettuce was grown in unlimed and limed quartz sand with nitrate as the sole source of nitrogen. Liming decreased lettuce yields but had no effect on Fe concentrations, indicating that Fe concentrations were a poor indicator of Fe bioavailability within the plant. In unlimed sand, utilization of ⁵⁹Fe from all ⁵⁹Fe‐chelates was on the same level (2.8%–3.6%). In limed sand, only ⁵⁹Fe‐EDDHA maintained the ⁵⁹Fe utilization on a level (3%) comparable to that in unlimed sand. Although the utilization of ⁵⁹Fe from the other chelates decreased to 0.6%–1.1% after liming, Fe concentrations were not affected due to the increased uptake of indigenous Fe. The most biodegradable form of EDDS, namely ⁵⁹Fe‐EDDS(S,S), provided ⁵⁹Fe for lettuce as efficiently as the mixture of ⁵⁹Fe‐EDDS stereoisomers and the ⁵⁹Fe‐EDTA. Utilization of ⁵⁹Fe in inorganic form was 0.5% and 0.03% in unlimed and limed sand, respectively. This study shows that biodegradable ligands are able to serve as chelators to sustain Fe availability in calcareous environments. They may be of use especially in drip irrigation, where ligand accumulations may pose a threat to groundwater quality.     

Multilayer Perceptron Neural Network Approach to Estimate Chlorophyll Concentration Index of Lettuce (Lactuca sativa L.)

Nitrogen is an essential nutrient for greenhouse-grown lettuce (Lactuca sativa L.); however, excessive nutrient availability causes disease and detrimental effects on the leaf and root development. In this study, nitrogen content of the lettuce leaves was estimated by determining the chlorophyll concentrations of the leaves using image processing technique. The Hoagland solution was used as a fertilizer in five different doses (control, quarter of the solution, half of the solution, standard solution, and two times more of the solution). Multilayer perceptron neural network (MLPNN) model was developed based on the red, green, and blue components of the color image captured to estimate chlorophyll content and chlorophyll concentration index (SPAD values). According to the obtained results, the MLPNN model was capable of estimating the lettuce leaf chlorophyll content with a reasonable accuracy. The coefficient of determination was 0.98, and mean square error was 0.006 in validation process.     

Utilization of some organic wastes as growing media for lettuce (Lactuca sativa L.) plants

Abstract

The environmental impact of crop wastes and the high cost of peat moss (PM) force scientists to find alternative growing media. In a pot experiment, peanut shell (PS) and corn wastes (CWs) were evaluated as growing media in comparison with PM in three different mixing ratios with washed sand. The mixing ratio were M₁?=?1:1 raw material to sand, M₂?=?1:2 raw material to sand, and M₃?=?1:3 raw material to sand. The tested plant was lettuce (Lactuca sativa L.). The obtained results showed that PS medium contained available nitrogen (N) and phosphorus (P) higher by 121% and 38% above the PM medium. Availability of potassium (K) in CW medium was higher by 167% than PM. The EC, pH, OC, and C/N ratio of PM were higher by 227%, 4%, 128%, and 99% above the CW and by 1,473%, 9%, 74%, and193% above the PS, respectively. The highest significant values of growth parameters were recorded in PM medium. The highest total cost and lowest net profit were recorded with the use of PMM₁, whereas the lowest cost and the highest net profit were obtained with PSM₂. Physiochemical characteristics of peanut shell suggested that it can be economical alternative growing media for PM.     

Effect of phosphorus‐enriched biochar and poultry manure on growth and mineral composition of lettuce (Lactuca sativa L. cv.) grown in alkaline soil

The use of pyrolysis products of manures gives positive effects on soil fertility, crop productivity and soil carbon sequestration. However, effects depend on soil characteristics, plant species and the raw material from which the biochar is derived, and some negative effects of biochar have been reported. The objective of this study was to evaluate the effectiveness of poultry manure (PM)‐derived biochar on the growth, and P, N, K, Ca, Mg, Fe, Zn, Cu and Mn concentration of lettuce (Lactuca sativa L.) plant. The treatments as follows: control, 20 g/kg poultry manure (PM), 20 g/kg phosphorus‐enriched poultry manure (PM+P), 10 g/kg Biochar (B), 10 g/kg Biochar+P (B+P). Application of biochar and PM significantly increased lettuce growth, and P‐enriched forms of PM and biochar gave the higher growth. PM has no significant effect on the N concentrations but biochar and, P‐enriched PM and biochar treatments significantly increased N concentrations. Phosphorus concentration of the lettuce leaves significantly increased by PM and biochar treatments. Plant K concentrations were also increased by PM and biochar, and their P‐enriched forms. Leaf Ca and Mg concentrations were lower in Biochar and B+P treatments than that of PM and PM+P treatments. Compared to control and PM treatments, biochar applications reduced Fe, Zn, Mn and Cu concentrations of the lettuce plants. The results of this study indicated that application of biochar to alkaline soil is beneficial for crop growth and N, P and K nutrition, but it certainly reduced Fe, Cu, Zn and Mn nutrition of lettuce.     

Effect of methyl jasmonate on phenolic compounds and carotenoids of romaine lettuce (Lactuca sativa L.)

The effect of exogenous methyl jasmonate (MeJA) on antioxidative compounds of romaine lettuce ( Lactuca sativa L.) was investigated. Lettuces were treated with various MeJA solutions (0, 0.05, 0.1, 0.25, and 0.5 mM) before harvest. Total phenolic compounds content and antioxidant capacity of romaine lettuce significantly increased after MeJA treatments (0.1, 0.25, and 0.5 mM). The total content of phenolic compounds of the romaine lettuce treated with 0.5 mM MeJA (31.6 microg of gallic acid equivalents/mg of dry weight) was 35% higher than that of the control. The increase in phenolic compound content was attributed to a caffeic acid derivative and an unknown phenolic compound, which also contributed to increased antioxidant capacity. The induction of phenylalanine ammonia-lyase (PAL) activity by the MeJA treatment indicated that phenolic compounds were altered due to the activation of the phenylpropandoid pathway. Total content of carotenoids, including lutein and beta-carotene, of the MeJA-treated lettuce did not change after 8 days of treatment, whereas the content of the control without MeJA decreased after 8 days. This research indicated that preharvest application of MeJA could increase the nutritional value of romaine lettuce under determined conditions discussed in this work.     

Roles of glycine betaine in mitigating deleterious effect of salt stress on lettuce (Lactuca sativa L.)

This study was conducted to evaluate the roles of glycine betaine (GB) in mitigating deleterious effect of salt stress on lettuce. Lettuce plants were subjected to two salinity (0 and 100 mmol l^?1 NaCl) and four GB levels (0, 5, 10, 25 mmol l^?1). Salinity resulted in a remarkable decrease in growth parameters, relative leaf water content and stomatal conductance. Plants subjected to salt stress exhibited an increase in membrane permeability (MP), lipid peroxidation (MDA), leaf chlorophyll reading value, H₂O₂ and sugar content. Exogenous foliar applications of GB reduced MP, MDA and H₂O₂ content in salt-stressed lettuce plants. Salt stress increased Na and generally decreased other nutrient elements. GB reduced Na accumulation, but significantly increased other element contents under salinity conditions. The study showed that gibberellic acid (GA) and salicylic acid (SA) content in salt-stressed plants were lower than those of nonstressed plants. However, salinity conditions generally increased the abscisic acid content. GB treatments elevated the concentrations of GA, SA and indole acetic acid (IAA) at especially 10 and 25 mmol l^?1 GB under salt stress conditions. It could be concluded that exogenous GB applications could ameliorate the harmful effects of salt stress in lettuce.     

Salt in irrigation water affects the nutritional and visual properties of romaine lettuce (Lactuca sativa L.)

The influence of salinity stress on the growth, appearance, and nutritional compounds, especially phenolic compounds and carotenoids, of romaine lettuce (Lactuca sativa L.), a low salt tolerant plant, was studied. The dry weight, height, and color of the lettuce plants were significantly changed by long-term irrigation (15 days) with higher NaCl concentration (i.e., >100 mM). However, no significant differences were observed in the growth and appearance among the control, all short-term treatments (2 days; 50, 100, 500, and 1000 mM), and long-term irrigation with low salt concentration. Moreover, in romaine lettuce treated with long-term irrigation with 5 mM NaCl, the total carotenoid content increased without color change, and the contents of major carotenoids in romaine lettuce, lutein and beta-carotene, increased 37 and 80%, respectively. No differences were observed in lutein and beta-carotene contents in short-term-treated lettuce. The phenolic content of the romaine lettuce declined with short-term salt irrigation, whereas there were no significant differences among treatments exposed to long-term irrigation. This research indicates that long-term irrigation with relatively low salt concentration, rather than short-term irrigation with high salt concentration, can increase carotenoid content in romaine lettuce without causing a tradeoff in yield or visual quality.     

Application of biosolids to soil affects Cu and Zn accumulation and antioxidant activity of lettuce (Lactuca sativa L.)

The application of biosolids to agricultural fields is becoming increasingly common. The effect of biosolids on the behavior of metals in different plants has been reported to be variable and ambiguous. A greenhouse experiment was conducted on a soil spiked with four rates of copper (Cu) (0, 50, 100, and 150 mg kg^?1) and zinc (Zn) (0, 150, 300, and 450 mg kg^?1) in lettuce culture. When Cu and Zn were spiked to anaerobically-digested biosolids, their availability in lettuce increased. Root and shoot fresh weight decreased due to a decrease in photosynthetic rate. superoxide dismutase (SOD) and peroxidases (POD) activity increased after the application of Cu and Zn. The bioavailability of Zn in lettuce was greater than that of Cu because of a higher transfer factor in plants and due to a higher endogenous Zn concentration.These results will allow for better fertilization management when biosolids are applied to tomato culture.     

Calculation of the total nitrate uptake of lettuce (Lactuca sativa L.) by use of a mathematical model to simulate nitrate inflow

Lettuce was grown in nutrient solution in a climate chamber under varied levels of temperature and radiation and in the greenhouse under fluctuating climatic conditions. A model to calculate maximum nitrate inflow (Imax) in relation to relative growth rate (RGR) and root:shoot ratio (RSR) was used for computing total-N uptake. For doing this, developing of submodels to calculate RGR and RSR was necessary. The good agreement between calculated N uptake and measured total-N taken up by the plants supports the assumption that Imax reflects the inflow necessary to meet the demand of plants.     

Macronutrients Composition of Lettuce Plants (Lactuca sativa L.) as Affected by Mineral Nutrition Level and ION Exchange Substrate Biona-312 Supplementation

The effect of ion exchange substrate Biona-312 addition (2 or 5%) on the macronutrients composition of butterhead lettuce cv. ‘Justyna’ plants under conditions of basic (1.5-times) and intensive (3-times concentrated Hoagland solution) mineral nutrition level was investigated. Both experimental doses of Biona-312 introduced into 1.5-times concentrated Hoagland nutrient solution [electrical conductivity (EC) 2.41–2.47 dS m^?1] dropped nitrogen (N) content in roots. Simultaneously statistically proven increase in foliar concentration of total sulfur (S) as well as a decrease in phosphorus (P) and potassium (K) contents in lettuce above- and underground organs were observed. The changes in calcium (Ca) and magnesium (Mg) content were insignificant. Ion exchange substrate supplementation into 3-times concentrated Hoagland nutrient solution (EC 6.85–7.30 dS m^?1), significantly elevated N and K contents in above- and underground organs, raised the foliar S content, decreased Ca and Mg contents in leaves, as well as dropped P concentration in roots and increased content in leaves.     

有机无机氮肥配施对菜地土壤二氧化碳和甲烷排放的影响

采用静态箱—气相色谱法研究不同种类有机无机肥料配合施用对盆栽莴苣土壤二氧化碳(CO_2)和甲烷(CH_4)排放规律及排放量的影响。以等氮(N)用量为基准,磷(P)、钾(K)用量相应折算,分别以猪粪、沼渣沼液、猪粪堆肥与化肥进行配施,且各处理N、P、K总施用量一致。共设置6个处理:不施肥(CK);不施氮肥(PK);施纯化肥(NPK);有机无机肥配施1(20%猪粪N+80%化肥N,NPKM1);有机无机肥配施2(20%沼渣沼液N+80%化肥N,NPKM2);有机无机肥配施3(20%猪粪堆肥N+80%化肥N,NPKM3)。结果表明:莴苣全生育期内土壤CH_4排放各处理呈现"源"、"汇"交替现象,各时间测点排放通量在每次施肥后均呈现升降波动趋势,直至总体趋于平稳;各阶段排放累积量随着生育期的变化,处理间差异逐渐明显。莴苣盆栽土壤CH_4累积排放量为-5.90～-0.42 kg·hm~(-2),大小表现为CK=PKNPKNPKM3NPKM1NPKM2。莴苣全生育期内土壤CO_2的各时间测点排放通量总体呈现上升趋势,受施肥影响略有波动;各阶段排放累积量随着生育期的增加,处理间差异更加明显。莴苣盆栽土壤CO_2累积排放量为1 506.37～2 260.58 kg·hm~(-2),大小表现为NPKM3NPKM1NPKNPKM2PKCK。莴苣盆栽全球增温潜势为1.50～2.23 t CO_2eq·hm~(-2),大小表现为NPKM3NPKM1NPKNPKM2PKCK。总体而言,不同有机无机肥料配施表现为菜地土壤的CH_4汇,以沼渣沼液配施效果最佳,而猪粪类有机肥配施会导致菜地全球增温潜势升高,应合理搭配进行施用。     

Effect of synthetic nano-hydroxyapatite as an alternative phosphorus source on growth and phosphorus nutrition of lettuce (Lactuca sativa L.) plant

Phosphorus (P) is one of the least available mineral nutrients to the plants in calcareous and alkaline soils. In this study, we investigated the synthesis, characterization and use of synthetic nano-hydroxyapatite (NHA), P uptake by plants as well as its residual effects. Soluble P source (H₃PO₄) was also included as treatment for comparison. NHA prepared by wet chemical techniques, characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and elemental dispersive X-ray (EDX) analysis. NHA and H₃PO₄ were applied at a rate of 200 mg kg^?1 to find out their effects on phosphorus nutrition and growth of lettuce on the low and high calcareous soil. In addition to this, residual effects of NHA and H₃PO₄ were also determined for lettuce plants grown after first lettuce plants in the both soil. Dry weights of the first and second lettuce plants grown in low and high calcareous soil were significantly increased by applied phosphorus regardless of the source. NHA seems to be more effective than that of ordinary phosphorus source (H₃PO₄-P) on growth and P concentration of the lettuce plants. The promising results of this study needs to be supported with long term field studies regarding the uptake, translocation and interactions of nano-P with the other elements.     

Strong increase of foliar inulin occurs in transgenic lettuce plants (Lactuca sativa L.) overexpressing the Asparagine Synthetase A gene from Escherichia coli

Transgenic lettuce (Lactuca sativa L. cv. 'Cortina') lines expressing the asparagine synthetase A gene from Escherichia coli were produced to alter the plant nitrogen status and eventually enhance growth. The relative molecular abundance of water-soluble metabolites was measured by 1H NMR in transgenic and conventional plants at early developmental stages and grown under the same conditions. NMR metabolic profiles assessed that a transgenic line and the wild-type counterpart shared the same compounds, but it also revealed side effects on the carbon metabolism following genetic modification. Concerning the nitrogen status, the amino acid content did not vary significantly, except for glutamic acid and gamma-aminobutyric acid, which diminished in the transgenics. As for the carbon metabolism, in transgenic leaves the contents of sucrose, glucose, and fructose decreased, whereas that of inulin increased up to 30 times, accompanied by the alteration of most Krebs's cycle organic acids and the rise of tartaric acid compared to nontransformed controls. Lettuce leaf inulins consisted of short oligomeric chains made of one glucose unit bound to two/four fructose units. Inulins are beneficial for human health, and they are extracted from plants and commercialized as long-chain types, whereas the short forms are synthesized chemically. Hence, lettuce genotypes with high content of foliar short-chain inulin represent useful materials for breeding strategies and a potential source for low molecular weight inulin.     

Peat replacement in horticultural growth media: the adequacy of coir,paper sludge and biogas digestate as growth medium constituents for tomato (Solanum lycopersicum L.) and lettuce (Lactuca sativa L.)

Purpose: Due to environmental concerns, efforts are made to replace the use of peat in horticultural growth media by organic wastes. Four growth media were prepared with the purpose of achieving adequate physical and chemical properties for plant production. Materials and methods: Growth media prepared from mixtures of coir (C) and paper sludge (P), respectively, with two biogas digestates from food waste (D₁ and D₂), were tested. These mixtures, 20% D₁ or D₂?+?80% C or P (v/v), were evaluated as growth media for tomato (Solanum lycopersicum L.) and lettuce (Lactuca sativa L.). Results and conclusion: The growth media were all physically stable during the growing period, provided all the macronutrients and most of the micronutrients necessary for plant growth, adequate pH conditions, as well as an adequate electrical conductivity. The mixture of D₂ and P produced the highest biomass compared to a mineral fertilised peat (control), with a biomass production of 76% of the control for lettuce and 54% for tomato. Causes for the biomass reduction relative to the control may be related to ammonium toxicity effects, and/or limited plant-available water. The digestates, particularly D₁, seemed also to have a phytotoxic effect on the germination.     

Humic substances can modulate the allelopathic potential of caffeic, ferulic, and salicylic acids for seedlings of lettuce (Lactuca sativa L.) and tomato (Lycopersicon esculentum Mill.)

The capacity of a leonardite humic acid (LHA), a soil humic acid (SHA), and a soil fulvic acid (SFA) in modulating the allelopathic potential of caffeic acid (CA), ferulic acid (FA), and salicylic acid (SA) on seedlings of lettuce (Lactuca sativa L.) and tomato (Lycopersicon esculentum Mill.) was investigated. Lettuce showed a sensitivity greater than that of tomato to CA, FA, and SA phytotoxicity, which was significantly reduced or even suppressed in the presence of SHA or SFA, especially at the highest dose, but not LHA. In general, SFA was slightly more active than SHA, and the efficiency of the action depended on their concentration, the plant species and the organ examined, and the allelochemical. The daily measured residual concentration of CA and FA decreased drastically and that of SA slightly in the presence of germinating seeds of lettuce, which were thus able to absorb and/or enhance the degradation of CA and FA. The adsorption capacity of SHA for the three allelochemicals was small and decreased in the order FA > CA > SA, thus suggesting that adsorption could be a relevant mechanism, but not the only one, involved in the "antiallelopathic" action.     

Preliminary results of a German field trial with different hemp (Cannabis sativa L.) accessions

Hemp is one of the oldest crops in the world and can be used in very different ways. Hemp cultivation was forbidden in Germany since 1982. Since 1996 hemp with less than 0.3 per cent THC content can be grown. To evaluate the available genetic hemp resources 14 monoecious and 26 dioecious hemp varieties and strains were grown in the field in 1996 and analysed for morphological traits. A large variability was found for plant height at different dates, blooming time, stem diameter, fresh and dry weight and seed yield. Plant density and plant height at maturity were negatively correlated. The initial growth rate is only of minor importance. There was no interaction between genotype and sex for dioecious accessions, though differences between sex types were important.