Persistency and monosaccharide composition of polysaccharides of soil which received no plant materials for a certain period under field conditions

Abstract

The monosaccharides in acid hydrolysates were monitored in soil which was packed in a bag made of glass microfiber paper and buried in upland and paddy fields for up to 36 months. During the initial flush of decomposition, all constituent monosaccharides except for non cellulosic glucose were found to decrease. The amounts which disappeared were greater than the water extractable saccharides of the air dried soil or ground sample of the air dried soil. After the flush of decomposition, the changes in mannose, galactose, fucose and rhamnose were small, whereas cellulosic glucose, arabinose and xylose continued to decline in the upland field soil. The soil saccharides are classified into six groups and their relative persistency is discussed.

The monosaccharide composition did not change markedly, but the proportion of monosaccharides relating to plant materials declined with time after incubation. The molar ratios of hexoses to pentoses, deoxyhexoses to pentoses, and non-cellulosic glucose to cellulosic glucose increased gradually, whereas a gradual decline in the ratio of xylose to mannose was observed when the soil received no plant materials under field conditions.     

Degradation of metolachlor in soil inoculated with a mixed fungal culture

The effect of a mixed fungal culture on the degradation of the herbicide metolachlor in soil was evaluated. Metolachlor was found to degrade up to 92% and 87% after 20 days in sterile and nonsterile soils respectively, when inoculated with a mixed culture of Aspergillus flavus and Aspergillus terricola and treated with 20 g g^–1 metolachlor. When the soil was treated at 50 g g^–1 level, 84% of the added herbicide degraded in inoculated sterile soil and 80% in nonsterile soil. The half-lives of metolachlor were found to be 5 and 4 times faster at the 20 and 50 g g^–1 levels, respectively, in the presence of the mixed fungal culture. In treated soils, four metabolites could be identified by RP-HPLC.     

黑土长期定位试验原状土搬迁对土壤真菌群落多样性的影响

采用PCR-DGGE及其特异性条带克隆测序的方法,研究黑土长期定位试验原状土整体搬迁对土壤真菌群落结构多样性的影响。对同为小麦茬的搬迁前(2010年)和搬迁后(2013年)土壤真菌群落结构的研究表明:DGGE图谱中搬迁前后2个土层不同处理的样品间条带的数量和亮度存在部分变化;对DGGE图谱进行聚类分析可得,真菌群落结构在2个土层各处理中搬迁前后有部分变化,其中MNPK处理的变化较为显著;对DGGE图谱主成分分析结果可得,在0~20 cm土层中各处理虽然搬迁前和搬迁后产生分异,但搬迁前后的3年中处理间的分布规律相似,在20~40 cm土层中搬迁前和搬迁后MNPK和CK处理的分布规律发生了变化。搬迁前和搬迁后土壤真菌群落结构受到搬迁扰动的影响远小于耕作方式、植被类型、土壤肥力等的影响。     

北京八达岭地区典型林分林地土壤抗蚀性分析

为研究八达岭地区不同森林植被对土壤抗蚀性的影响,揭示不同林分林地土壤抗蚀性特征,以该地区典型植被类型(油松林、针阔混交林、阔叶林和灌木林)为研究对象,运用主成分分析及相关分析方法,研究该区域内,不同林分类型土壤的抗蚀性.结果表明:八达岭地区林分林地土壤干筛时,团聚体主要集中于5～2 mm粒径范围内,在0.5-0.25 mm粒径范围内质量分数最少;湿筛后,＜0.25 mm小粒径范围内质量分数最大,在10～5 mm大粒径范围内质量分数最少.为比较各典型林分林地土壤抗蚀性,选取土壤理化性质、土壤团聚特征等12种指标,以主成分分析方法为基础,确定评价北京八达岭地区土壤抗蚀性的最佳指标体系,同时,经相关分析选定干筛时,土壤几何平均直径(GMD)为八达岭地区各典型林分林地最佳土壤抗蚀指标.经分析,八达岭地区各典型林分土壤表层(0～10 cm)抗蚀性由强到弱依次为阔叶林＞灌木林＞针阔混交林＞油松林;对于主要发生土壤侵蚀的0～20 cm土壤表层抗蚀性由强到弱为灌木林＞油松林＞阔叶林＞针阔混交林;在各土层平均值综合情况下,评价结果选取抗蚀性综合指数表示,得出各林地土壤抗蚀性由强到弱依次为灌木林＞油松林＞针阔混交林＞阔叶林.     

Response of ectomycorrhizal communities to past Roman occupation in an oak forest

The impact of past Roman occupation on the composition of ectomycorrhizal (ECM) communities was analysed in 12 Roman settlements in an oak forest in Central France. At each Roman settlement, soils and ECM roots were sampled from two plots (600 m² each), one plot close to the remains of the buildings (<100 m), supposed to be impacted by ancient Roman agriculture, and the second plot 250-500 m away from the remains of the buildings, supposed to be less intensively influenced by previous cultivation. Soils were analysed and ECM fungal taxa were identified by morphotyping and sequencing the rDNA ITS region. The soil properties were significantly affected by the past Roman occupation, in terms of nutrient availability, especially for P, N and Mg. The enhancement of soil nutrient levels by past Roman land-use had significantly modified alpha diversity and species composition of ECM communities. Among the 67 determined ECM morphotypes, 40 were shared by the occupied and non-occupied plots, 17 were found only in the occupied plots and 10 only in the non-occupied plots. Six morphotypes were significantly more frequent near the antique remnants. Our study showed, for the first time, that ectomycorrhizal communities are impacted by previous Roman land-use, even after nearly two thousand years of forest state.     

重庆缙云山林地枯落物及土壤水文效应研究

 为了解中亚亚热带常绿阔叶林地的土壤状况,以重庆缙云山4种林分为研究对象,通过标准地调查、土壤物理性质及持水能力测定和入渗实验,对林地枯落物和土壤水分效应进行研究。结果表明:1)缙云山林地土壤具有较强的持水能力,1m深土壤持水能力为9.5013.17 mm。2)枯落物蓄积量为16.21～32.42t/hm2,枯落物持水率为针阔混交林>灌木林>常绿阔叶林>楠竹林,持水量为灌木林>针阔混交林>常绿阔叶林>楠竹林。3)菲利浦模型较好地反映研究地区不同类型林地土壤入渗过程。     

Decoupled stoichiometric,isotopic, and fungal responses of an ectomycorrhizal black spruce forest to nitrogen and phosphorus additions

Many northern forests are limited by nitrogen (N) availability, slight changes in which can have profound effects on ecosystem function and the activity of ectomycorrhizal (EcM) fungi. Increasing N and phosphorus (P) availability, an analog to accelerated soil organic matter decomposition in a warming climate, could decrease plant dependency on EcM fungi and increase plant productivity as a result of greater carbon use efficiency. However, the impact of altered N and P availability on the growth and activity of EcM fungi in boreal forests remains poorly understood despite recognition of their importance to host plant nutrition and soil carbon sequestration. To address such uncertainty we examined above and belowground ecosystem properties in a boreal black spruce forest following five years of factorial N and P additions. By combining detailed soil, fungal, and plant δ¹⁵N measurements with in situ metrics of fungal biomass, growth, and activity, we found both expected and unexpected patterns. Soil nitrate isotope values became ¹⁵N enriched in response to both N and P additions; fungal biomass was repressed by N yet both biomass and growth were stimulated by P; and, black spruce dependency on EcM derived N increased slightly when N and P were added alone yet significantly declined when added in combination. These findings contradict predictions that N fertilization would increase plant P demands and P fertilization would further exacerbate plant N demands. As a result, the prediction that EcM fungi predictably respond to plant N limitation was not supported. These findings highlight P as an under appreciated mediator of the activity of denitrifying bacteria, EcM fungi, and the dynamics of N cycles in boreal forests. Further, use of δ¹⁵N values from bulk soils, plants, and fungi to understand how EcM systems respond to changing nutrient availabilities will often require additional ecological information.     

沂蒙山林区不同森林群落土壤水分贮存与入渗特征

为给沂蒙山区森林植被建设中合理群落类型的选择与配置提供参考,采用森林水文学方法,对淮河流域沂蒙山林区典型森林群落的土壤水分贮存与人渗特征进行研究。结果表明：1）森林群落具有显著提高林地土壤孔隙度、土壤贮水量和土壤入渗速率的作用,其作用程度混交林大于纯林,阔叶林大于针叶林;2）森林群落对土壤（非毛管）滞留贮水量的提高程度明显大于土壤毛管吸持贮水量,因而具有明显的涵养水源作用;3）Horton模型和Kostiakov模型能较好模拟研究区森林群落的土壤人渗过程,但通用经验模型和Philip模型的适用性较差;土壤入渗速率与孔隙度之间具有明显的线性正相关关系。     

Dispersal of microarthropods in forest soil

This experiment was a part of a research on the soil community in anthropogenous birch stands in Finland. In that study it was found that communities of Collembola are similar in birch stands of different origin (cultivated field or spruce forest), while the communities of Oribatida are essentially different. When compared to original spruce forest, the communities of both groups are different.Cultivation eliminates the populations of most microarthropod species, that have to disperse after reforestation from the surrounding areas. The aim of the experiment was to study the ambulatory dispersal of soil microarthropods. It was carried out in plastic boxes filled with an intact block of defaunated surface soil taken from a birch stand that was established ca. 30 years earlier on a cultivated field. A strip of intact spruce forest soil was placed at one end of the boxes to harbour the source populations. At the opposite end we placed a row of pitfall traps. Soil samples were taken every two weeks at increasing distances from the source soil using a metal corer, and animals were extracted in a high gradient apparatus. The pitfalls were also emptied every two weeks. The experiment lasted for ten weeks.Pitfall and soil sample data gave an estimation of the maximum dispersal rate for each species or genus. The distance that the populations could potentially disperse in 30 years (age of the stand) indicated that some, but not all of the species could have actively migrated to the central parts of the birch stand (30 m).     

Response of soil respiration to nitrogen addition in two subtropical forest types

Anthropogenic activities have increased nitrogen (N) deposition in terrestrial ecosystems, which directly and indirectly affects soil biogeochemical processes, including soil respiration. However, the effects of the increases in N availability on soil respiration are not fully understood. In this study, soil respiration was measured using an infrared gas analyzer system with soil chambers under four N treatments (0, 5, 15, and 30 g N m^-2 year^-1 as control, low N (LN), moderate N (MN), and high N (HN), respectively) in camphor tree and slash pine forests in subtropical China. Results showed that soil respiration rates decreased by 37% in the camphor tree forest and 27% in the slash pine forest on average on an annual base, respectively, in the N-fertilized treatments when compared with the control. No significant differences were found in the soil respiration rate among the LN, MN, and HN treatments in both forest types as these fertilized plots reached an adequate N content zone. In addition, soil microbial biomass carbon (C) content and fine root biomass declined in N-treated plots compared to the control. Our results indicated that elevated N deposition might alter the tree growth pattern, C partitioning, and microbial activity, which further affect soil C sequestration by reducing soil respiration in subtropical forests of China.     

长期有机无机肥配施对东北黑土真菌群落结构的影响

【目的】分析长期有机无机肥配施对土壤真菌丰度、多样性及其群落特征的影响,探讨东北黑土真菌群落变化与施肥的相关性,为进一步调节土壤微生物结构,改善其生态功能提供参考依据。【方法】依托黑龙江省农业科学院36年长期定位试验站,选取4个不同施肥处理:不施肥处理（CK）、有机肥处理（M）、氮磷钾无机肥处理（NPK）和有机肥配施无机肥处理（MNPK）的耕作层土壤为研究对象,以真菌ITS基因为分子标靶借助qPCR技术和Illumina Miseq高通量测序平台,研究不同施肥处理对黑土中真菌群落丰度、多样性和组成的影响,并与土壤化学性质进行偶联分析,揭示群落与施肥的相关性。【结果】长期施用无机肥显著降低土壤pH,而有机无机配施可以有效缓解土壤酸化。NPK处理的ITS基因丰度显著高于MNPK;MNPK处理的细菌/真菌比值（26.91104）显著大于NPK,各处理比值由高到低为MNPK M CK NPK。细菌/真菌比值与土壤pH正相关;MNPK处理的真菌多样性指数值略大于NPK。Ascomycota和Zygomycota为土壤中主要真菌门,不同施肥处理之间真菌组成的相对丰度存在显著差异,对照处理Ascomycota的相对丰度为45.35%,MNPK和NPK处理分别为50.93%和56.16%。有机肥有利于降低病原真菌相对丰度,具有高度侵染性的Cochliobolus在MNPK（0.41%）和M（0.39%）中的相对丰度显著小于CK（3.25%）和NPK（2.08%）。CCA分析表明,土壤理化性质共解释土壤真菌群落结构变化的73.3%,有效磷（贡献量为32.4%,P=0.002）、铵态氮（贡献量为14.8%,P=0.01）和硝态氮（贡献量为16.2%,P=0.048）是3个重要的影响因子。【结论】不同施肥条件下土壤真菌丰度、多样性,以及菌群组成特征不同。与无机肥相比,有机肥无机肥配施能够有效改善真菌群落结构,降低真菌的丰度,增加真菌多样性,并提高土壤pH,减缓土壤酸化。土壤有效磷、铵态氮和硝态氮含量是影响黑土土壤真菌群落结构变化重要因素。     

Composition of the microbial communities in the mineral soil under different types of natural forest

Phospholipid fatty acid (PLFA) patterns were used to describe the composition of the soil microbial communities under 12 natural forest stands including oak and beech, spruce-fir-beech, floodplain and pine forests. In addition to the quantification of total PLFAs, soil microbial biomass was measured by substrate-induced respiration and chloroform fumigation-extraction. The forest stands possess natural vegetation, representing an expression of the natural site factors, and we hypothesised that each forest type would support a specific soil microbial community. Principal component analysis (PCA) of PLFA patterns revealed that the microbial communities were compositionally distinct in the floodplain and pine forests, comprising azonal forest types, and were more similar in the oak, beech and spruce-fir-beech forests, which represent the zonal vegetation types of the region. In the nutrient-rich floodplain forests, the fatty acids 16:1ω5, 17:0cy, a15:0 and a17:0 were the most prevalent and soil pH seemed to be responsible for the discrimination of the soil microbial communities against those of the zonal forest types. The pine forest soils were set apart from the other forest soils by a higher abundance of PLFA 18:2ω6,9, which is typical of fungi and may also indicate ectomycorrhizal fungi associated with pine trees, and high amounts of PLFA 10Me18:0, which is common in actinomycetes. These findings suggest that the occurrence of azonal forest types at sites with specific soil conditions is accompanied by the development of specific soil microbial communities. The study provides information on the microbial communities in undisturbed forest soils which may facilitate interpretation of data derived from managed or even damaged or degraded forests.     

Extramatrical mycelia of ectomycorrhizal fungi as moderators of carbon dynamics in forest soil

Extramatrical mycelia (EMM) of ectomycorrhizal (ECM) fungi are potentially extensive in soil and receive significant allocations of plant-derived carbon. Although losses from living EMM occur via respiration and exudation, EMM represents a considerable biomass component and potential carbon sink in many forest soils. ECM root tips and rhizomorphs may persist in soil for many months, but interactions between grazing arthropods and decomposers probably facilitate more rapid turnover of diffuse EMM. Elevated atmospheric CO₂ concentration [CO₂] is likely to increase carbon allocation to ECM fungi by their tree hosts. This will probably increase root colonization by ECM fungi and drive changes in their communities in soil. The likely effects of elevated [CO₂] and other climate change factors on the production and turnover of EMM production are difficult to predict from current evidence, and this hampers our understanding of their potential value as future carbon sinks. Responses of grazing soil arthropods to future climate change will have a strong influence on EMM turnover, along with the abilities of ECM fungi to store carbon in below-ground, and this should be seen as a priority area for future research.     

Relationships between soil properties and feeding activity of soil fauna in acid forest soils

We investigated the relationships between soil chemical properties, humus form, and feeding activity in eight forest sites in Northrhine‐Westphalia, Germany. The study sites varied in forest type (oak, oak‐hornbeam, spruce, and pine). Three study sites were located under the same climatic conditions, and five study sites were distributed all over Northrhine‐Westphalia. We determined humus form, soil chemical properties, and feeding activity in three replication plots per site. We used the bait‐lamina test to determine feeding activity of soil fauna. Independent of forest type, all study sites were very acid with pH(CaCl₂) values in the Ah horizon between 2.8 and 4.0. The three study sites located under the same climatic conditions showed very homogenous soil chemical properties (pH in Ah: 2.9–3.0), whereas the five other sites varied significantly due to their soil chemical parameters (pH in Ah: 2.8–4.0). All single sites presented feeding activities with a very low spatial heterogeneity. Forest types and climatic conditions were not related to activity of soil fauna. Feeding activity and thickness of the O layer were strongly negatively correlated, whereas the activity was strongly positively correlated to soil pH in the five study sites with a wide range of soil chemical parameters. The three extremely acid forest sites presented significant differences in feeding activities that were not related to soil chemical properties.     

北京西山地区林火对土壤性质的影响

 为进行火烧迹地的管理,采用调查取样和室内分析方法,对北京西山地区不同林火强度下油松林土壤物理和化学性质的变化进行了研究。结果表明:与未火烧相比,中度火烧会显著提高土壤密度,显著降低土壤总孔隙度、土壤黏粒含量和田间持水量,而毛管孔隙和非毛管孔隙的变化却不明显;轻度火烧对土壤物理性质的影响不显著。轻度火烧会显著地提高0～5cm范围的土壤有机质含量,而中度火烧会显著降低土壤有机质和全氮含量。轻度火烧使土壤pH值增加,其中0～2cm的差异达到显著水平;另外,0～5 cm土层Ca²⁺和Mg²⁺的含量比中度火烧和未火烧土壤有明显的提高。因此,轻度火烧有利于改善土壤的性质。     

福建闽江上游不同林地类型土壤水库“库容”的特性

 以裸露地为对照,研究木荷林地、杉木林地、封山育林地的土壤水库“库容”组成及其特性,以探讨森林土壤水库的蓄水调水机制。结果表明:木荷林地、杉木林地和封山育林地土壤水库总“库容”,分别为503.95、471.62和451.48mm,分别是裸露地的1.23、1.15、1.10倍;兴利库容分别为326.87、332.21和309.94mm,分别是裸露地的1.23、1.25、1.17倍;死库容分别为114.29、89.60和101.46mm,分别是裸露地的1.11、0.87、0.99倍;防洪库容分别为62.79、49.81和40.08mm,分别是裸露地的1.52、1.21、0.97倍。最大有效库容为389.66、382.02和350.02mm,分别是裸露地的1.27、1.25、1.14倍。不同林地类型土壤水库的“库容”组成,均表现为兴利库容>死库容>防洪库容。与裸露地相比,林地土壤水库“库容”的增加,与林木生长对林地土壤结构的改良作用和林地地表的良好覆盖有关。     

大兴安岭林区落叶松林地不同发育阶段土壤肥力演变与评价

 以大兴安岭林区不同发育阶段兴安落叶松人工林和天然次生林为研究对象,采用野外调查和定量分析相结合的方法,对林地土壤有机质、土壤腐殖质组成、土壤酸度指标及其主要养分含量的变化进行测定和分析。利用主成分分析的方法,以各主成分特征贡献率为权重,加权计算各林地土壤肥力综合指标值。结果表明:随着林龄的增长,不同发育阶段落叶松人工林土壤酸度总体呈现升高的趋势,土壤有酸化的迹象;全磷和速效钾呈现降低的趋势;有机质、水解氮、胡敏酸和胡敏素含量在近熟林略有升高,但总体呈现降低的趋势。土壤肥力评价结果为:天然林>幼龄林>近熟林>中龄林>成熟林,即随着林龄的增长,土壤肥力迅速下降,其主要原因是林地凋落物分解缓慢。因此,建议通过适度提高林地抚育强度,调整群落结构,增加林地光照,诱导天然阔叶树种进入,形成针阔混交林的调控措施,调节落叶松人工纯林凋落物分解与积累的矛盾,以维持地力平衡和提高林地生产力。