Impacts of pellet pH on nitrous oxide emission rates from cattle manure compost pellets

Previous reports indicated that the emission of nitrous oxide (N₂O) when manure compost pellets (MCP) were applied to soil was greater than when ordinary manure compost or inorganic fertilizer was applied, but that applying pellets of nitrogen-enriched manure compost, a by-product of deodorizing manure during composting, resulted in N₂O emission rates less than those from MCPs. To investigate the mechanism by which N₂O emission rates and cumulative emissions were reduced in nitrogen-enriched manure composts pellets (N+MCP), we studied the impact of pellet pH on N₂O emission, because pH is different between MCP (pH 8.6) and N+MCP (pH 5.3). In an incubation experiment, the pH of pellets was adjusted to five levels (5.3, 6.0, 7.0, 8.0 and 8.6) with acid or alkaline solutions, and the pellets were incubated without soil in a beaker at 30°C for 90 d (MCP) or 42 d (N+MCP). A large peak in N₂O emission rate was observed soon after beginning the incubation (within 1–3 d) in the neutral and alkaline treatments for both MCP and N+MCP, and these peaks corresponded to a rise in the pellet nitrite contents. Thus, this N₂O emission peak might have been generated by the denitrification of nitrite in the pellets. In the acid treatments of MCP, the N₂O emission was distributed more in the later incubation period (14–90 d), when the reduction of nitrate in MCP occurred. This led to a significant increase in cumulative N₂O emission as compared with the alkaline treatments for MCP. Regarding the mechanism by which N₂O emission was reduced in N+MCP, although larger cumulative N₂O emission rates in the earlier stage (0–14 d for MCP and 0–7 d for N+MCP) were observed when the pellet pH was adjusted close to 7.0, lowering the pH of MCP to 5.3 (the pH of N+MCP) did not demonstrate a significant decrease in cumulative N₂O emission as compared with the original pH treatment (pH 8.6). These results indicate that pellet pH might not relate directly to the mechanism by which N₂O emission was reduced in N+MCP.     

Effects of soil type and nitrate concentration on denitrification products (N2O and N2) under flooded conditions in laboratory microcosms

Abstract

Denitrification products nitrous oxide ((N₂O) and nitrogen (N₂)) were measured in three flooded soils (paddy soil from Vietnam, PV; mangrove soil from Vietnam, MV; paddy soil from Japan, PJ) with different nitrate (NO₃^–) concentrations. Closed incubation experiments were conducted in 100-mL bottles for 7 d at 25°C. Each bottle contained 2 g of air-dried soil and 25 mL solution with NO₃^– (concentration 0, 5 or 10 mg N L^?1) with or without acetylene (C₂H₂). The N₂O + N₂ emissions were estimated by the C₂H₂ inhibition method. Results showed that N₂O + N₂ emissions for 7 d were positively correlated with those of NO₃^– removal from solution with C₂H₂ (R² = 0.9872), indicating that most removed NO₃^– was transformed to N₂O and N₂ by denitrification. In PJ soil, N₂O and N₂ emissions were increased significantly (P < 0.05) by the addition of greater NO₃^– concentrations. However, N₂O and N₂ emissions from PV and MV soils were increased by the addition of 0 to 5 mg N L^?1, but not by 5 to 10 mg N L^?1. At 10 mg N L^?1, N₂ emissions for 7 d were greater in PJ soil (pH 7.0) than in PV (pH 5.8) or MV (pH 4.3) soils, while N₂O emissions were higher in PV and MV soils than in PJ soil. In MV soil, N₂O was the main product throughout the experiment. In conclusion, NO₃^– concentration and soil pH affected N₂O and N₂ emissions from three flooded soils.     

Aeration effects on CO2, N2O,and CH4 emission and leachate composition of a forest soil

The availability of O₂ is one of the most important factors controlling the chemical and biological reactions in soils. In this study, the effects of different aeration conditions on the dynamics of the emission of trace gases (CO₂, N₂O, CH₄) and the leachate composition (NO₃^‐, DOC, Mn, Fe) were determined. The experiment was conducted with naturally structured soil columns (silty clay, Vertisol) from a well aerated forest site. The soil monoliths were incubated in a microcosm system at different O₂ concentrations (0, 0.001, 0.005, 0.01, 0.05, and 0.205 m³ m^‐3 in the air flow through the headspace of the microcosms) for 85 days. Reduced O₂ availability resulted in a decreased CO₂ release but in increased N₂O emission rates. The greatest cumulative N₂O emissions (= 1.6 g N₂O‐N m^‐2) were observed at intermediate O₂ concentrations (0.005 and 0.01 m³ m^‐3) when both nitrification and denitrification occurred simultaneously in the soil. Cumulative N₂O emissions were smallest (= 0.05 g N₂O‐N m^‐2) for the aeration with ambient air (O₂ concentration: 0.205 m³ m^‐3), although nitrate availability was greatest in this treatment. The emission of CH₄ and leaching of Mn and Fe were restricted to the soil columns incubated under completely anoxic conditions. The sequence of the reduction processes under completely anoxic conditions complied with the thermodynamic theory: soil nitrate was reduced first, followed by the reduction of Mn(IV) and Fe(III) and finally CO₂ was reduced to CH₄. The re‐aeration of the soil columns after 85 days of anoxic incubation terminated the production of CH₄ and dissolved Fe and Mn in the soil but strongly increased the emission rates of CO₂ and N₂O and the leaching of NO₃^‐ probably because of the accumulation of DOC and NH₄⁺ during the previous anoxic period.     

秸秆还田的小麦–玉米农田 N2O 周年排放的量化分析

【目的】N₂O 是重要的温室气体之一,主要来源于农田土壤。华北平原是我国的粮食主产区,秸秆还田是该地区主要的农田管理措施,明确不同秸秆还田量对小麦玉米农田周年土壤温度和含水量的影响以及与 N₂O 排放之间的量化关系,对发挥秸秆还田的生态效应,明确硝化和反硝化作用机制具有重要意义。【方法】以冬小麦、夏玉米为研究对象,设置 5 种不同秸秆还田量处理:小麦、玉米秸秆均不还田 (T0);小麦秸秆 1875 kg/hm2 + 玉米秸秆 2000 kg/hm2 还田 (T1);小麦秸秆 3750 kg/hm2 + 玉米秸秆 4000 kg/hm2 还田 (T2);小麦秸秆 5625 kg/hm2 + 玉米秸秆 6000 kg/hm2 还田 (T3);小麦秸秆 7500 kg/hm2 + 玉米秸秆 8000 kg/hm2 还田 (T4)。于 2014 年 10 月～2015 年 10 月,采用静态箱–气相色谱法对农田 N₂O 排放进行测定,探究不同秸秆还田量下小麦玉米农田 N₂O 排放的周年变化,并量化分析土壤温度、含水量与 N₂O 排放的关系。【结果】秸秆还田量显著影响 N₂O 的排放,随着秸秆还田量的增加,周年内 N₂O 排放总量呈增加的趋势,增加量为 1.33～3.50 kg/hm2,增加率为 32.3%～85.0%;通量增加量为 15.52～40.87 μg/(m2·h),增加率为 32.3%～85.1%。玉米季 N₂O 排放通量和总量分别是小麦季的 2.42～2.62 和 1.05～1.14 倍。秸秆还田可提高 0—10 cm 土壤温度和 0—20 cm 土壤含水量,增加范围分别为 0.63～2.14℃ 和 0.6%～1.8%。相关性分析表明,各处理土壤温度和 N₂O 排放通量无相关关系(P > 0.05)。T0、T1、T2 处理土壤含水量与 N₂O 排放通量呈显著正相关(P < 0.05),而 T3、T4 处理与 N₂O 排放通量之间不相关(P > 0.05)。【结论】随着秸秆还田量的增加,N₂O 排放通量和总量均呈现增加趋势,且玉米季高于小麦季。秸秆还田显著促进 N₂O 排放并可提高 0—20 cm 土壤含水量和 0—10 cm 土壤温度,周年秸秆还田量在 7750 kg/hm2 及以下时,N₂O 排放通量与土壤含水量之间呈显著正相关,而与土壤温度之间不相关。     

污泥及其混合堆肥对番茄土壤性质和N_2O排放的影响

目前关于污泥及其生物质堆肥的土地利用过程中土壤性质变化和温室气体排放数据十分缺乏,难以满足农田土壤氮素保存和温室气体减排的需求。该研究通过在番茄种植过程中添加800 kg/hm2新鲜污泥(S-H)、400 kg/hm2新鲜污泥(S-L)、800 kg/hm2秸秆堆肥(VM-S)和800 kg/hm2猪粪堆肥(VM-M),开展土壤性质、无机氮形态、作物生长以及N2O排放特征的研究。结果表明:堆肥处理显著增加了土壤电导率(electric conductivity,EC)(P0.05),其中猪粪堆肥时土壤EC值最大。添加污泥和堆肥都使土壤p H值显著上升(P0.05),最终趋于中性,且VM-M对土壤酸化的抑制效果略优于VM-S。污泥和堆肥处理时土壤NO3--N浓度显著高于对照,且各处理组NO3--N浓度均随时间逐渐下降,NO3--N主要被番茄吸收,部分NO3--N从土壤上层淋溶至下层;NH4+大多数被氧化为NO3-,部分NH4+被植物吸收。在施入的无机氮量相等情况下,VM-M、VM-S、S-H处理组中番茄地上部分生物量分别为1 515、1 383、1 103 g/株,株高分别为56.8、54.5、51.3 cm,对番茄生长的促进效果为VM-MVM-SS-H,而S-H比S-L多施入的氮肥对番茄生长并未起到明显促进作用(P0.05)。与对照相比,污泥或生物质堆肥都显著提高了土壤N2O的排放(P0.05),各处理组N2O的排放均集中于施肥后的前20天,且土壤N2O的排放通量大小顺序为S-L(0.76 kg/(hm2·a))VM-M(0.95 kg/(hm2·a))VM-S(1.19 kg/(hm2·a))S-H(1.71 kg/(hm2·a))。因此,在进行污泥及其生物质堆肥的土地利用时,应考虑有机肥的种类及其施用量,以在提高作物产量的同时改善土壤并减少温室气体排放,在进行污泥的农田利用时可先将污泥与畜禽粪堆肥。     

Lime-nitrogen application reduces N2O emission from a vegetable field with imperfectly-drained sandy clay-loam soil

Abstract

We studied the effect of lime-nitrogen (calcium cyanamide, CaCN₂) application on the emission of nitrous oxide (N₂O) from a vegetable field with imperfectly-drained sandy clay-loam soil. Lime-nitrogen acts as both a pesticide and a fertilizer. During the decomposition of lime-nitrogen in the soil, dicyandiamide (DCD), a nitrification inhibitor, is formed, and as a result lime-nitrogen application may mitigate N₂O emission from the soil. The study design consisted of three different nitrogen-application treatments in field plots with a randomized block design. The nitrogen application treatments were: CF (chemical fertilizer), LN (all nitrogen fertilizer applied as lime-nitrogen), and CFD (chemical fertilizer containing DCD). Soil nitrification activity was lower in the LN and CFD plots than in the CF plots, and nitrification was inhibited for a longer period in the LN plots than in the CFD plots. In the LN plots, N₂O emission was lower than those of other treatments from 20 to 40 days after fertilization, a period when large peaks of N₂O emission were observed after rainfall in the CF and CFD plots. Cumulative N₂O emission over 63 days in the CF plots (mean ± standard deviation: 30.2 ± 14.4 mg N₂O m^?2) and CFD plots (24.3 ± 10.8 mg N₂O m^?2) was significantly higher than that in the LN plots (10.7 ± 1.2 mg N₂O m^?2; P < 0.05). Our results suggested that lime-nitrogen application decreased N₂O emission by inhibiting both nitrification and denitrification.     

No significant difference in N2O emission,fertilizer-induced N2O emission factor and CH4 absorption between anaerobically digested cattle slurry and chemical fertilizer applied timothy (Phleum pratense L.) sward in central Hokkaido,Japan

Abstract

Nitrous oxide (N₂O) and methane (CH₄) fluxes from a fertilized timothy (Phleum pratense L.) sward on the northern island of Japan were measured over 2?years using a randomized block design in the field. The objectives of the present study were to obtain annual N₂O and CH₄ emission rates and to elucidate the effect of the applied material (control [no nitrogen], anaerobically digested cattle slurry [ADCS] or chemical fertilizer [CF]) and the application season (autumn or spring) on the annual N₂O emission, fertilizer-induced N₂O emission factor (EF) and the annual CH₄ absorption. Ammonium sulfate was applied to the CF plots at the same application rate of NH₄-N to the ADCS plots. A three-way ANOVA was used to examine the significance of the factors (the applied material, the application season and the year). The ANOVA for the annual N₂O emission rates showed a significant effect with regard to the applied material (P?=?0.042). The annual N₂O emission rate from the control plots (0.398?kg N₂O-N ha^?1?year^?1) was significantly lower than that from the ADCS plots (0.708?kg N₂O-N ha^?1?year^?1) and the CF plots (0.636?kg N₂O-N ha^?1?year^?1). There was no significant difference in the annual N₂O emission rate between the ADCS and CF plots. The ANOVA for the EFs showed insignificance of all factors (P?>?0.05). The total mean?±?standard error of the EFs (fertilizer-induced N₂O-N emission/total applied N) was 0.0024?±?0.0007 (kg N₂O-N [kg N]^?1), which is similar to the reported EF (0.0032?±?0.0013) for well-drained uplands in Japan. The CH₄ absorption rates differed significantly between years (P?=?0.014). The CH₄ absorption rate in the first year (3.28?kg CH₄?ha^?1?year^?1) was higher than that in the second year (2.31?kg CH₄?ha^?1?year^?1), probably as a result of lower precipitation in the first year. In conclusion, under the same application rate of NH₄-N, differences in the applied materials (ADCS or CF) and the application season (autumn or spring) led to no significant differences in N₂O emission, fertilizer-induced N₂O EF and CH₄ absorption.     

Effects of phosphorus addition on N2O and NO emissions from soils of an Acacia mangium plantation

Abstract

An incubation experiment was conducted to examine the effects of the phosphorus (P) application on nitrous oxide (N₂O) and nitric oxide (NO) emissions from soils of an Acacia mangium plantation in Indonesia. The soils were incubated with and without the addition of P (Ca[H₂PO₄]₂; 2 mg P g soil)^?1) after adjusting the water-filled pore space (WFPS) to 75% or 100%. The P addition increased N₂O emissions under both WFPS conditions and NO emissions at 75% WFPS. Some possible mechanisms are considered. First, the P addition stimulated nitrogen (N) cycling, and N used for nitrification and/or denitrification also increased. Second, the P addition could have relieved the P shortage for nitrifying and/or denitrifying bacteria, producing N₂O and NO. Our results suggest that the application of P fertilizer has the potential to stimulate N₂O and NO emissions from Acacia mangium plantations, at least when soils are under relatively wet conditions.     

适宜施氮量降低京郊小麦-玉米农田N2O排放系数增加产量

为明确京郊地区小麦-玉米轮作农田的N_2O排放特征,寻求既能减少N_2O排放又保证粮食产量的切实有效措施,以京郊地区冬小麦-夏玉米轮作农田为研究对象,运用静态箱法对8个施氮水平的农田N_2O交换通量进行了连续一年对比研究,每季作物施肥量分别为N0(0 kg/hm~2),N1(50 kg/hm~2),N2(100 kg/hm~2),N3(150 kg/hm~2),N4(200 kg/hm~2),N5(250 kg/hm~2),N6(300 kg/hm~2),和N7(400 kg/hm~2)。在N0-N7施氮量条件下冬小麦季N_2O排放量为0.08~0.52 kg/hm~2;夏玉米季0.26~3.70 kg/hm~2。整个轮作周期,小麦季各处理N_2O排放损失率为0.05%~0.13%;玉米季0.78%~1.02%。在京郊地区冬小麦-夏玉米轮作体系中夏玉米季氮肥施入农田土壤后,土壤N_2O排放通量高于小麦季。京郊农田土壤N_2O排放通量表现出明显的季节性和日变化规律。综合考虑本试验条件下施肥量、N_2O排放量和京郊地区潮土农田小麦-玉米产量,研究认为该轮作体系中每季作物的施肥量为N4(200 kg/hm~2)比较合理,可为合理施肥及估算中国农田温室气体排放量提供参考。     

适宜节水灌溉模式抑制寒地稻田N_2O排放增加水稻产量

2014年在大田试验条件下,设置控制灌溉、间歇灌溉、浅湿灌溉及淹灌4种水分管理模式,采用静态暗箱-气相色谱法田间观测寒地水稻生长季N2O排放特征,研究不同灌溉模式对寒地稻田N2O排放的影响及N2O排放对土壤环境要素的响应,同时测定水稻产量,以期为寒地稻田N2O排放特征研究提供对策。结果表明:不同灌溉模式下N2O排放的高峰均出现在水分交替频繁阶段,水稻生育阶段前期,各处理N2O排放都处于较低水平,泡田期几乎无N2O排放。与淹灌相比,间歇灌溉使N2O排放总量增加47.3%,控制灌溉和浅湿灌溉使N2O排放总量减少40.7%和39.6%。寒地稻田N2O排放通量与土壤硝态氮含量关系密切,与土壤10 cm温度显著相关(P0.05)。水稻生长期间各处理N2O排放顺序间歇灌溉淹灌,二者均显著高于浅湿灌溉和控制灌溉(P0.05)。各处理水稻产量以浅湿灌溉最低、其他方式差异不显著。可见,间歇灌溉有助于提高水稻产量,但会促进稻田N2O的排放。在综合考虑水稻产量及稻田温室效应的需求下,控制灌溉为最佳灌溉方式,应予以高度重视。该研究可为黑龙江寒地稻作区选择节水减排模式提供科学支撑。     

菜地土壤CO2与N2O排放特征及其规律

为了解不同集约化类型菜地土壤CO2和N2O排放特征及影响因子,选取京郊20年露地老菜地(OV20)、3年菜地种植历史的露地新菜地(OV3)、3年大棚菜地(GV3),以及相邻的当地典型粮田玉米地(Maize)4个类型地块,研究了春黄瓜生育期间土壤CO2和N2O排放特征及影响因子。结果表明:1)春黄瓜生育期间的土壤CO2排放通量主要受土壤5 cm处温度(指数关系)和土壤水分(对数关系或二次抛物线关系)影响;期间玉米地土壤CO2平均排放通量为(346.8±56.5)mg.m-2.h-1,20年露地菜地、3年露地菜地有机肥处理、3年露地菜地配施处理、3年大棚菜地的土壤CO2平均排放通量分别是玉米地的1.38、1.21、1.39和1.56倍。2)土壤N2O排放通量与施肥活动密切相关,排放高峰都出现在氮肥施用后,并受土壤温度和水分的影响。基肥后土壤温度低(15～20℃),排放峰出现在第5 d,排放峰持续时间(长达20 d)与施肥量相关;追肥后土壤温度高(>20℃),排放高峰发生早(追肥后第3 d),但因追肥用量低,因此持续时间短(仅一周)。3)黄瓜生长期内玉米地N2O累积排放量为N(1.95±0.10)kg.hm-2,20年老菜地、3年大棚菜地和3年新菜地N2O累积排放量分别是同期大田玉米地的1.67、1.95和1.99倍。4)本实验中春黄瓜生长季菜地土壤化肥氮N2O排放系数在1.86%～4.71%之间,显著高于IPCC旱地排放缺省值1%。其中,新菜地排放系数高于老菜地,设施菜地排放系数高于露地菜地;但有机肥氮的N2O排放系数则远远低于化肥氮的排放系数,仅为0.11%。     

硝态氮源及碳源有效性对土壤N_2O和CO_2排放的影响

以华北平原农田土壤为对象,通过室内静态培养系统研究NO_3~--N与不同碳源组合对土壤N_2O和CO_2排放的影响。结果表明,NO_3~--N作为氮源和不同碳源施入土壤,除NO_3~-+纤维素,其余土壤N_2O排放通量均高于对照组和只添加氮源土壤;NO_3~--N和不同碳源组合的CO_2累积排放量均高于对照和只添加氮源土壤。NO_3~-+果胶的N_2O排放量在第1 d达到最大值1 383.42μg N·kg~(-1)·d~(-1);NO_3~-+葡萄糖的CO_2排放量在第1 d达到最大值370.13 mg C·kg~(-1)·d~(-1),CO_2累积排放量顺序为:葡萄糖果胶秸秆纤维素淀粉木质素。土壤NO_3~--N含量与N_2O排放呈极显著正相关。总之,添加纤维素可以抑制N_2O的排放,促进CO_2排放,并增加土壤中NO_3~--N含量,添加其余碳源均会促进土壤N_2O和CO_2排放。     

The potential for N2O emission and nitrate leaching in seasonally open solar greenhouses during the summer fallow: a 15N tracer study

The mechanisms responsible for nitrous oxide (N₂O) emission and the potential for nitrate leaching under seasonally open solar greenhouses were investigated in a nitrogen‐15 (¹⁵N) tracer study during the summer fallow period in Yangling, Shaanxi Province, China. ¹⁵N‐labelled urea and KNO₃ were applied at 30 (U1, K1) and 60 (U2, K2) kg N/ha, respectively, to microplots in a solar greenhouse after harvest of tomatoes. Large N₂O emissions resulted from each treatment, with a total N₂O flux of 482, 1481, 2283, 656 and 991 g N/ha for control (CK), U1, U2, K1 and K2, respectively, were primarily observed during the first month of the summer fallow period, reflecting the combination of considerable residual nitrate, soil organic matter and rainfall. The total ¹⁵N‐N₂O flux was 35.2, 37.2, 7.9 and 11.2 g N/ha for U1, U2, K1 and K2, respectively, accounting for 0.54, 0.40, 0.17 and 0.12% of the applied N, suggesting that both nitrification and denitrification contributed to the enhanced N₂O emission. After 2 months of fallow, only 52.08–54.78% of applied ¹⁵N remained in the top 0–40 cm soil layer, where there was the main concentration of tomato roots. Of the total ¹⁵N‐labelled KNO₃ applied, 86.76% (K1) and 82.06% (K2) remained in the 0–100 cm soil layers and the rest of the N (about 13.07% for K1 and 17.82% for K2, calculated by subtracting ¹⁵N‐N₂O loss and ¹⁵N remaining in the 0–100 cm soil layers from the total applied ¹⁵N) leached below 100 cm. Solar greenhouses in north‐west China clearly have great potential for both N₂O emission and nitrate leaching during the summer fallow period.     

夏玉米农田N2O排放影响因素的模拟分析

【目的】全球46%~52%的N2O来自农田土壤,农田土壤N2O排放的研究具有重要的环境和经济意义。量化各影响因素对夏玉米农田N2O排放的影响,可为合理减少施肥产生的N2O排放提供依据。【方法】于2012和2013年连续两年进行了夏玉米裂区田间试验。试验主区为作物处理,副区为氮肥处理(0、 150、 300、 450 kg/hm2)。采用暗箱静态法-气相色谱法测定了不同处理N2O的排放通量,比较了不同温度和降雨量条件下不同处理的N2O排放量,计算了气温、 降雨量、 氮肥管理和夏玉米吸收对夏玉米农田N2O排放的影响。【结果】温度及降雨量的变化明显影响N2O的排放。2012年和2013年气温和降雨量对夏玉米生长期间N2O总排放量的影响分别为-0.24和-0.07。随着施氮量的增加,施氮对N2O排放的影响率呈线性增加(R2 = 0.923),施氮量0、 150、 300和450 kg/hm2,对玉米田N2O排放的影响分别为0、 0.38、 1.63、 3.54。夏玉米生长吸收对N2O排放量的平均影响因子为-0.33,年际间差异不显著(P = 0.07)。在苗期、 穗期、 花粒期,夏玉米生长吸收的影响因子分别为-0.57、 -0.29和-0.13,不同生育期的影响因子差异显著(P = 0.0015)。不同施氮量下,气候条件对夏玉米农田N2O排放影响率差异不显著(P 0.05); 不同气温和降雨量,夏玉米生长吸收对N2O排放的影响在同一施氮量下差异不显著(P 0.05),且均随施氮量的增加而减小。【结论】通过量化分析,气候条件对N2O排放的影响与气温和降雨量密切相关,温度升高影响增大,反之则减小,降雨后排放显著增大。施氮对N2O排放的影响随施氮量增加线性增加。夏玉米生长吸收降低了N2O排放,且在不同生育时期的影响差异显著。综合各影响因子,低氮量条件下(≦150 kg/hm2),气候因素和玉米生长对N2O排放的影响较大,高氮量下(≧300 kg/hm2),氮肥的施用是影响N2O排放的主要因子。     

Short‐term N2O,CO2, NH3 fluxes,and N/C transfers in a Danish grass‐clover pasture after simulated urine deposition in autumn

Urine patches are significant hot‐spots of C and N transformations. To investigate the effects of urine composition on C and N turnover and gaseous emissions from a Danish pasture soil, a field plot study was carried out in September 2001. Cattle urine was amended with two levels of ¹³C‐ and ¹⁵N‐labeled urea, corresponding to 5.58 and 9.54 g urea‐N l^–1, to reflect two levels of protein intake. Urine was then added to a sandy‐loam pasture soil equivalent to a rate of 23.3 or 39.8 g urea‐N m^–2. Pools and isotopic labeling of nitrous oxide (N₂O) and CO₂ emissions, extractable urea, ammonium (NH₄⁺), and nitrate (NO₃^–), and plant uptake were monitored during a 14 d period, while ammonia (NH₃) losses were estimated in separate plots amended with unlabeled urine. Ammonia volatilization was estimated to account for 14% and 12% of the urea‐N applied in the low (UL) and high (UH) urea treatment, respectively. The recovery of urea‐derived N as NH₄⁺ increased during the first several days, but isotopic dilution was significant, possibly as a result of stress‐induced microbial metabolism. After a 2 d lag phase, nitrification proceeded at similar rates in UL and UH despite a significant difference in NH₄⁺ availability. Nitrous oxide fluxes were low, but generally increased during the 14 d period, as did the proportion derived from urea‐N. On day 14, the contribution from urea was 23% (UL) and 13% (UH treatment), respectively. Cumulative total losses of N₂O during the 14 d period corresponded to 0.021% (UL) and 0.015% (UH) of applied urea‐N. Nitrification was probably the source of N₂O. Emission of urea‐derived C as CO₂ was only detectable within the first 24 h. Urea‐derived C and N in above‐ground plant material was only significant at the first sampling, indicating that uptake of urine‐C and N via the leaves was small. Urine composition did not influence the potential for N₂O emissions from urine patches under the experimental conditions, but the importance of site conditions and season should be investigated further.     

添加DCD对雨养区春玉米产量、 氧化亚氮排放及硝态氮残留的影响

【目的】田间条件下氮肥中添加硝化抑制剂双氰胺(dicyandiamide,DCD)对氧化亚氮排放和硝态氮含量变化的影响尚不清楚,研究不同施氮模式对玉米产量、 氧化亚氮排放以及对土壤深层硝态氮残留和氮肥农学效率等的影响,对提高氮肥利用效率、 减少农业源温室气体排放具有重要意义。【方法】试验于2013年4月至2014年9月,在中国科学院长武黄土高原农业生态试验站进行,供试作物为春玉米,品种为先玉335,半覆膜种植。试验设4个处理: 传统施氮(Con)、 减量施氮(Opt)、 减量施氮+硝化抑制剂(Opt+DCD)、 不施氮(N0),定期采集土样和氧化亚氮气体,利用流动分析仪和气象色谱仪测定土壤矿质氮和氧化亚氮的含量,采用SAS软件对不同处理的产量和各个指标进行方差分析。【结果】Opt和Opt+DCD在保持产量的同时,显著影响氧化亚氮排放和硝态氮残留。Opt处理的硝态氮含量峰值降低13.7%,而Opt+DCD处理降低硝态氮峰值19.0%。其次,施氮模式还影响硝态氮峰值出现的时间: Con(190.1 mg/kg)率先出现, 其次是Opt(164.0 mg/kg)和Opt+DCD(132.9 mg/kg)。Opt显著降低了氧化亚氮的排放,降幅为29.4%,而在Opt基础上添加DCD可进一步降低氧化亚氮排放28.1%。在雨季,硝态氮含量和氧化亚氮排放随降雨而出现波动。尽管Opt降低了铵态氮峰值,但在Opt基础上,添加DCD提高了铵态氮峰值。4种施氮模式土壤剖面0100 cm和100200 cm的硝态氮残留量分别介于33.5~148.9和24.8~92.8 kg/hm2之间,平均值分别为78.5和56.4 kg/hm2。土壤剖面0200 cm的硝态氮残留量以Con最大,为225.9 kg/hm2,与Con相比,Opt和Opt+DCD的硝态氮残留量降幅分别为48.0%~59.0%、 29.4%~57.5%。Opt和Opt+DCD之间硝态氮残留差异不显著(P0.05)。【结论】不同施氮模式对玉米产量、 矿质氮和氧化亚氮的动态变化以及氮肥农学效率具有显著影响,但是Opt和Opt+DCD在施氮量减少20%的同时,不仅没有显著降低玉米产量,还进一步降低了土壤剖面硝态氮的残留量和农业源温室气体的排放。因此在黄土高原雨养农业区,添加DCD是一种科学有效的施肥管理方式。     

Effect of the long-term application of organic matter on soil carbon accumulation and GHG emissions from a rice paddy field in a cool-temperate region,Japan-II. Effect of different compost applications-

ABSTRACT

The influence of long-term application of different types of compost on rice grain yield, CH₄ and N₂O emissions, and soil carbon storage (0 ? 30 cm) in rice paddy fields was clarified. Two sets of paddy fields applied with rice straw compost or livestock manure compost mainly derived from cattle were used in this study. Each set comprised long-term application (LT) and corresponding control (CT) plots. The application rates for rice straw compost (42 years) and livestock manure compost (41 years in total with different application rates) were 20 Mg fresh weight ha^–1. Soil carbon storage increased by 33% and 37% with long-term application of rice straw compost and livestock manure compost, respectively. The soil carbon sequestration rate by the organic matter application was 23% higher with the livestock manure compost than with the rice straw compost. The rice grain yield in the LT plot was significantly higher than that in the corresponding CT plot with both types of compost. Although the difference was not significant in the rice straw compost, cumulative CH₄ emissions increased with long-term application of both composts. Increase rate of CH₄ emission with long-term application was higher in the livestock manure compost (99%) than that in the rice straw compost (26%). In both composts, the long-term application did not increase N₂O emission significantly. As with the rice straw compost, the increase in CH₄ emission with the long-term application of livestock manure compost exceeded the soil carbon sequestration rate, and the change in the net greenhouse gas (GHG) balance calculated by the difference between them was positive, indicating a net increase in the GHG emissions. The increase in CH₄ and net GHG emissions owing to the long-term application of the livestock manure compost could be higher than that of the rice straw compost owing to the amount of applied carbon, the quality of compost and the soil carbon accumulation. The possibility that carbon sequestration in the subsoil differs depending on the type of composts suggests the importance of including subsoil in the evaluation of soil carbon sequestration by long-term application of organic matter.     

基于动态箱法的北京延庆区牛粪堆放CH4和N2O排放量估算

畜禽粪便堆放管理会造成甲烷(CH_4)和氧化亚氮(N_2O)等温室气体的大量排放。通过联合国政府间气候变化专门委员会(IPCC)建议的排放系数等方法,可以实现对某一区域范围内畜禽粪便管理系统的温室气体排放总量的估算,但由于其排放受粪便管理、气候条件等因素的显著影响,直接套用IPCC的默认系数会产生较大的误差。为更加准确估算中国奶牛粪便管理所造成的CH_4、N_2O排放,该文在对北京延庆区奶牛生产与粪便管理模式进行了实地调研的基础上,采用动态箱法模拟了奶牛粪便不同季节短时自然堆放管理模式下的CH_4、N_2O排放过程,并对区域内的年温室气体排放总量进行了测算。研究结果表明,奶牛粪便在一个月的自然堆放管理模式下,每千克牛粪挥发性固体在春、夏、秋季的CH_4排放量分别为223.97、4 603.31、351.38 mg,每千克牛粪N_2O排放量分别为5.86、9.43、0.81 mg。2016年北京延庆区全年奶牛粪便CH_4、N_2O排放总量分别为13 342.50、347.87 kg。延庆区奶牛粪便堆放管理过程的CH_4排放因子为1.50kg/(头·a),小于IPCC指南中的1.78 kg/(头·a);受堆放时间较短的影响,N_2O的排放因子则显著小于IPCC的推荐值。若直接使用IPCC默认参数估算延庆区奶牛粪便堆放管理过程中的CH_4和N_2O排放量,会造成排放量的高估。     

The influence of compost and zeolite co‐addition on the nutrients status and plant growth in intensively cultivated Mediterranean soils

The main objective of the study was to test the benefits of compost and zeolite co‐addition on the fertility of organic‐rich Mediterranean soils. Previous pot study in greenhouse found that zeolites mixed with compost significantly improved potassium availability as well as exchangeable potassium capacity in the soils. To further test this finding, a field experiment was conducted using potato – Solanum tuberosum L., desiree cultivar in peat soils of the Hula Valley, Israel. Adhering to the protocol of the greenhouse experiments, the treatments included 5% compost addition with no zeolites, 2% zeolite addition without compost, co‐addition of 5% compost mixed with 2% zeolites and control. We found that compost addition increased significantly the potatoes yield and the number of large tubers; however, the zeolite addition had no impact on yield. Co‐addition of compost and zeolites did not improve total crop yield or number of large tubers compared with compost addition only. The results are consistent with nutrients availability (N, P, K) across the treatments. In a commercialized field using the experiment conditions, the 2% zeolite addition would amount to 18 ton of zeolites per hectare. Hence, we conclude that soil amendment with the tested zeolite might be beneficial to improve soil retention for cationic nutrients (e.g. K⁺) under high leaching systems such as plant culture in pots, but in the field with high loads of compost, its effect is minor.     

氮肥配施生化抑制剂对黄泥田土壤钾素淋溶特征的影响

中国南方黄泥田土壤中养分淋失严重,尤其是氮(N)和钾(K),不仅造成资源浪费和潜在环境威胁,还严重制约作物的可持续生产。采用室内土柱模拟培养,研究尿素(U)和尿素硝铵(UAN)中单独添加脲酶抑制剂N-丁基硫代磷酰三胺(NBPT)和硝化抑制剂2-氯-6-(三氯甲基)吡啶(CP),及两者配合施用对黄泥田土壤中K素淋溶特征的影响,探讨提高黄泥田供钾能力的施肥技术。不同氮肥种类淋溶液中,K~+平均浓度大小表现为UAN处理(103.0 mg·kg~(-1))高于U处理(93.9 mg·kg~(-1)),且抑制剂处理间存在明显差异。培养结束时(第72 d),UAN处理K~+淋失量较U处理高6.7%。U各处理淋溶液中K~+累积量大小表现为UU+NBPTU+NBPT+CPU+CPCK,其中U+NBPT、U+CP和U+NBPT+CP处理较U处理分别降低8.7%、20.2%和14.9%;UAN各处理淋溶液中K~+累积量表现为UANUAN+NBPTUAN+NBPT+CPUAN+CPCK,其中UAN+NBPT、UAN+CP和UAN+NBPT+CP处理较UAN处理分别降低6.0%、13.8%和9.2%。不同施肥处理K~+淋溶率表现为UANUAN+NBPTUUAN+NBPT+CPUAN+CPU+NBPTU+NBPT+CPU+CP。培养中期(第36 d),U和UAN处理肥际微域中土壤速效钾含量显著降低,而添加CP处理有效维持土壤中较高的速效钾含量。与单施NBPT相比,配施CP可以减少黄泥田土壤中NO_3~-淋溶,增加土壤晶格对K~+的固定,减轻K~+淋溶风险,有效时间超过72 d。对各处理淋溶液中K~+累积量(y)随NO_3~-累积量(x)的变化进行拟合,其中以线性方程(y=ax+b)和Elovich方程(y=alnx+b)的拟合度最高,且抑制剂处理间a、b值均存在明显差异。总之,在黄泥田土壤中单施CP,或与NBPT配施可以有效增加K~+吸附,降低土壤中K~+淋溶损失,减轻养分淋失风险,提高肥料利用率。