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1.
A 14‐week trial was conducted to investigate the effects of antinutritional factors (ANFs) commonly present in soybean ingredients, singly and in combination, on Atlantic salmon (Salmo salar L.) fed from start‐feeding. The experimental diets consisted of a negative control fish meal diet (FM), and a positive control diet with 167 g kg?1 soybean meal inclusion (SBM) and four diets based on the FM diet supplemented with 2 g kg?1 soya‐saponins (SAP), 1.5 g kg?1 isoflavones (IFL), 0.3 g kg?1 phytosterols (PHS) or a mixture of these (MIX). Fish fed the SAP diet showed significantly higher growth performance than those fed FM, while the IFL treatment significantly decreased growth performance of salmon fry. Fish fed the IFL diet had significantly lower maltase activity and higher trypsin activity in proximal intestine than fish fed the FM diet. Histological differences were observed in the liver of fish fed the IFL diet, characterized by reduced size of the hepatocytes. Fish fed the PHS and IFL diets showed the highest frequencies of skeletal deformities among the six treatments. In conclusion, the results indicate that purified isoflavones may negatively affect growth performance, intestinal function, liver metabolism and bone formation of salmon fry.  相似文献   

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Although dietary marine phospholipids are able to improve culture performance of marine fish larvae in a further extend than soybean lecithin, both types of phospholipids (PL) markedly increase oxidative risk. The inclusion of a fat‐soluble antioxidant such as the vitamin E α‐tocopherol could allow a better control of oxidative stress. The objective of this study was to determine the combined effect of graded levels of α‐tocopherol with different levels and sources of krill phospholipids (KPL) and soybean lecithin (SBL) on growth, survival, resistance to stress, oxidative status, bone metabolism‐related genes expression and biochemical composition of sea bream larvae. Sea bream larvae were completely weaned at 16 dph and fed for 30 days seven microdiets with three different levels of PL (0, 40 and 80 g kg?1 diet) and two of α‐tocopherol 1500 and 3000 mg kg?1 diet. Sea bream larvae fed diets without PL supplementation showed the lowest survival, growth and stress resistance, whereas increase in PL, particularly KPL, markedly promoted larval survival and growth. However, feeding SBL markedly increased TBARs and GPX gene expression increasing the peroxidation risk in the larvae. Besides, KPL inclusion improved incorporation of n‐3 HUFA and, particularly, EPA into larval tissues, these fatty acids being positively correlated with the expression of BMP‐4, RUNX 2, ALP, OC and OP genes and to bone mineralization for a given larval size class. The increase in dietary α‐tocopherol tends to improve growth in relation to the n‐3 HUFA levels in the diet, denoting the protective role of this vitamin against oxidation. Indeed, dietary α‐tocopherol decreased the oxidative stress in the larvae as denoted by the reduction in larval TBARs contents and gene expression of SOD and CAT, but not GPX. Thus, increase in dietary α‐tocopherol effectively prevented the formation of free radicals from HUFA, particularly EPA, but did not affect the incidence of bone anomalies or the expression of genes related to osteogenetic processes.  相似文献   

5.
To be able to study nutrient requirement and utilization in any species, a diet supporting normal feed intake and growth equally well as a traditional fish meal‐based diet is needed. Additionally the formulation of the diet should allow low levels of the nutrient under study. When studying the amino acid metabolism and requirements, one cannot rely on the fish meal‐based diets as fish meal are nicely balanced according to requirements. Therefore the current study aimed to develop a plant protein‐based diet (with low fish meal inclusion) to be used in the nutritional studies of Senegalese sole juveniles supporting feed intake and growth close to that obtained in a fish meal‐based control feed. Two experiments were conducted to evaluate whether Senegalese sole juveniles would accept and utilize diets containing high plant protein inclusion. For testing the acceptance of high plant protein inclusion, two diets were formulated: a reference diet that contained fish meal as the main protein source (450 g kg?1 dry matter) whereas in the test diet, fish meal was substituted by a mixture of plant ingredients (soybean meal, corn and wheat gluten) with l ‐lysine supplementation. In order to improve the palatability, 50 g kg?1 squid meal was added to both diets. The indispensable amino acids (IAA) profile of the test diet was made similar to the control diet by adding crystalline amino acids. Further, automatic feeders were used to improve the feed intake. Fish (24 g initial body weight) were fed the diets for a period of 4 weeks. As fish accepted both diets equally well, a second study was undertaken to test the growth performance. Fish (6 g initial BW) were fed the diets for a period of 12 weeks. The use of automatic feeders to deliver the feed and the addition of both squid and balancing the indispensable amino acids resulted in growth performance and accretion not differing from the fish meal fed control. It can be concluded that juvenile Senegalese sole are able to grow and utilize high plant‐protein diets when both diet composition and feeding regime are adequate for this species.  相似文献   

6.
The effect of varying dietary levels of defatted soybean meal on the growth and survival of mrigal, Cirrhinus mrigala (Hamilton) was investigated. In a feeding trial of 90 days, three experimental diets containing soybean meal at 200, 300 and 400 g kg?1 level of incorporation were fed to quadruplicate groups of 10 fish each. The conventional feed used in India, consisting of a mixture of groundnut oil cake and rice bran in 1 : 1 ratio served as the control. Best growth in terms of percentage weight gain, specific growth rate, protein efficiency ratio (PER), feed conversion ratio and survival rate was obtained for the test diet with 354 g kg?1 crude protein and with 400 g kg?1 soybean meal inclusion level. However, no statistical significant difference was observed between the three soybean‐based diets, except for PER and survival rate. Soybean meal is an easily available, acceptable and cost‐effective protein source in formulated feeds for Indian major carps. The results of the present study indicate that a diet of 350 g kg?1 overall protein with soybean meal included at 400 g kg?1 can elicit good growth response and survival in mrigal.  相似文献   

7.
This study compared the effect of increasing dietary inclusion of soybean, narrow‐leaf lupin or yellow lupin kernel meals when fed to rainbow trout. Each meal was formulated into a test diet to create a series of inclusion levels of 0, 100, 200, 300 and 400 g kg?1. Each diet was formulated to the same digestible protein and energy specifications. The diets were fed to apparent satiety to 30.0 ± 0.71 g rainbow trout for 28 days. After this period, fish in the reference (0 g kg?1) treatment attained a weight of 93.2 g. Growth of the fish among the treatments was observed to be improved by the addition of either variety of lupin meal. In contrast, growth of fish fed the soybean meal was equivalent to that of the 0 g kg?1 fish meal reference, but was poorer at the 400 g kg?1 inclusion level. Feed intake with the lupin meals was marginally improved with lupin inclusion, but at the higher soybean meal inclusions, feed intake was reduced relative to the fish meal reference diet. The inclusion of the different grains also had significant effects on the physical properties of the pellets.  相似文献   

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A 12‐week feeding trial was carried out in concrete tanks to examine complete and partial replacement (75%) of fish meal (FM) with poultry by‐product meal (PBM), meat and bone meal (MBM) and soybean meal (SBM) in practical feeds for African catfish Clarias gariepinus. Triplicate groups of fish (initial body weight ranged from 90.33 to 93.93 g fish−1) were fed seven isonitrogenous and isocaloric diets of 20% digestible protein and 300 kcal 100 g−1 of digestible energy. The control contained 25% herring meal, whereas in the other six diets, PBM, MBM and SBM replaced 75% or 100% of the FM. Final body weight (FBW) and specific growth rate (SGR) of the fish fed diets containing PBM (75% and 100%), SBM (75% and 100%) and MBM (75%) were all higher, but not significantly different than those for fish fed the control diet. Replacing 100% of the FM by MBM significantly lowered FBW and SGR. Concerning whole body composition, there were no significant differences in ash and gross energy content of whole‐body among fish; fish fed diets containing PBM‐100% recorded significantly lower protein content compared with the control diet, while fish fed diet SBM‐100% recorded significantly lower moisture content compared with the control diet. Also fish fed diets SBM‐100% and PBM‐75% recorded higher lipid and gross energy contents compared with the control diet. The study revealed that satisfactory growth and feed utilization responses could be achieved through the replacement of FM by PBM, SBM and MBM in the diet of African catfish.  相似文献   

10.
A long‐term feeding experiment was conducted to investigate the use of cottonseed and soybean meal (CS) with iron and phosphorus supplements in diets for olive flounder (Paralichthys olivaceus). Olive flounder with an initial average size of 28.5 ± 0.35 g (mean ± SD) were divided into 15 groups (three tanks per dietary treatment) and fed 480 g kg?1 crude protein diets in which each of five isonitrogenous diets was formulated to contain different levels of cottonseed/soybean meal (1 : 1) to replace fish meal (FM) with iron and phosphorus supplementations. The five experimental diets were as follows: diet 1 (control), 0%CS; diet 2, 20%CS; diet 3, 30%CS; diet 4, 30%CS + Fe&P; and diet 5, 40%CS + Fe&P. After 26 weeks of feeding trial, no significant differences were observed in weight gain, feed utilization and survival among all the treatments. The total gossypol accumulation in liver of fish fed diets supplemented with iron was significantly lower than that of fish fed diets without supplementation of iron. The results indicate that the addition of iron in diets could prevent the absorption of free gossypol. The findings in this study suggest that dietary supplements of iron and phosphorus could increase the inclusion of cottonseed and soybean meal for FM replacement in diets for marine fish species.  相似文献   

11.
A feeding trial was conducted to evaluate the potential of replacing fishmeal with poultry byproduct meal (PBM) and soybean meal in diets for largemouth bass, Micropterus salmoides. A reference diet (C) contained 400 g/kg fishmeal, and 40 or 60% of the fishmeal was replaced with a blend of pet‐food‐grade PBM and soybean meal (diets PP1 and PP2) or a blend of feed‐grade PBM and soybean meal (diets PF1 and PF2). No significant differences were found in weight gain, nitrogen retention efficiency (NRE), condition factor, and body composition among fish fed diets PP1, PP2, PF1, and PF2. Feed intake and feed conversion ratio (FCR) were higher in fish fed diet PF1 than in fish fed diet PP1. No significant differences were found in weight gain, NRE, condition factor, and body composition between fish fed diet C and diets PP1, PP2, PF1, and PF2. The feed intake and FCR were lower in fish fed diet C than in fish fed diets PP2, PF1, and PF2. This study reveals that dietary fishmeal level for largemouth bass could be reduced to 160 g/kg by inclusion of PBM and soybean meal in combination.  相似文献   

12.
The study was conducted to investigate the effects of soybean meal (SBM), raffinose and stachyose on juvenile crucian carp (Carassius auratus gibelio♀ × Cyprinus carpio♂). The experimental diets consisted of one control diet based on fish meal (FM), one diet containing 300 g kg?1 SBM and four FM‐based diets with the addition of either 6.7 g kg?1 raffinose (Raf), 33.9 g kg?1 stachyose (Sta), a combination of raffinose and stachyose (Raf?Sta) and finally a Raf?Sta diet supplemented with 2.5 g kg?1 saponins (Raf?Sta?Sap). After 3 weeks of feeding, the relative gut lengths of SBM‐fed fish and the fish fed stachyose‐containing diets were shorter than those of the FM‐fed fish; further, more SBM‐fed fish showed fissures on the tips of the intestinal folds. After 8 weeks of feeding, the growth of SBM‐fed fish was significantly lower than that of FM‐fed fish (P<0.05). The fish fed Raf?Sta?Sap had a low relative gut length (P<0.05). In comparison with the other fish, the SBM‐fed fish had a higher number of large‐sized homogeneous vacuoles in the cytoplasm of epithelial cells and shorter microvilli. No significant difference was observed in body composition or intestinal microflora. The results indicated that raffinose and stachyose played no or only minor roles in the development of soybean‐induced growth reduction.  相似文献   

13.
Triplicate groups of triploid and diploid Atlantic salmon were fed diets with a low (LP, total P: 7.1 g kg?1), medium (MP, total P: 9.4 g kg?1) or high (HP, total P: 16.3 g kg?1) phosphorous (P) level from first feeding (0.18 g) to transfer to sea water (~50 g, duration: 203 days) and subsequently fed a commercial diet in sea water for 426 days (~3 kg). This study examined the short‐ and long‐term effects of dietary P on freshwater performance (mortality, growth), vertebral deformities (radiology), bone cell activity (ALP and TRACP enzyme activity in vertebrae and scales, and fgf23, bgp and igf‐I relative gene expression in vertebrae), bone mineralization (ash content) and some parameters related to fish condition (heart and liver size). Irrespective of ploidy, at seawater transfer, fish fed the MP diet had significantly highest length and weight and those fed the LP diet significantly lowest length and weight, while those fed the HP diet had intermediate lengths and weights. Increased dietary phosphorus reduced deformities in both ploidies at seawater transfer; however, triploids fed the LP and MP diets had more deformities than diploids fed the respective diets, while there was no ploidy effect observed for fish fed the HP diet. The vertebral bone ash content at seawater transfer was significantly higher in diploids than in triploids when fed the MP diet only. Alkaline phosphatase (ALP) and tartrate‐resistant acid phosphatase (TRACP) enzyme activities and relative gene expression of bone hormones involved in metabolism of plasma phosphate (fgf23) and bone growth (bgp) were not affected by ploidy at seawater transfer, but by dietary P level; LP increased ALP activity and reduced TRACP activity and fgf23 and bgp expression levels in vertebral bone. In scales, LP increased both ALP and TRACP activity. At the termination of the seawater period, the group‐wise pattern in occurrence of vertebral deformities was the same as at seawater transfer. The present results on mortality, growth, bone mineralization and development of skeletal deformities all demonstrate that triploids have a higher P requirement than diploids in fresh water. This study shows that an optimalization of P nutrition for triploid Atlantic salmon can improve health and welfare and reduce down‐grading of triploid salmon.  相似文献   

14.
A commercially available soybean molasses (alcohol extract from soybean meal) was included at levels of 0. 5,10,15 and 20% in a fish meal based diet for Atlantic salmon, Salmo salar L., weighing about 75 g. These inclusion levels supplied dietary alcohol soluble components corresponding to levels that would have been obtained if a standard soybean meal was included at levels up to about 70%. The fish were reared in fresh water at 9oC and with a minimum of 7 ppm O2 at outlet, and fed the experimental diets for 14 days before the faeces were stripped. Digestibility coefficients were determined using chromic oxide as an indigestible indicator. The digestibility of fat, and particularly the long-chained, saturated-and monounsaturated fatty acids, were significantly reduced by increasing the inclusion level of soybean molasses, whereas digestibility of dry matter, protein (%N x 6.25) and polyunsaturated fatty acids were not affected significantly. The present results show that alcohol-soluble components of soybeans may be responsible for a significant part of the negative effects of standard soybean meal in diets for Atlantic salmon.  相似文献   

15.
The effect of partial substitution of dietary fish meal with a high‐value soybean meal (HVS) and a commercial soybean meal (CSM) on the growth performance in juvenile Japanese seabass (8.3±0.2 g body weight) was determined. Nine isonitrogenous (crude protein 44%) and isoenergetic (20 kJ g?1) practical diets replacing 0 (the control), 15%, 30%, 45% and 60% fish meal protein by soybean meal protein (HVS or CSM) were formulated. Each diet was randomly assigned to four replicate cages (1.5 × 1.5 × 2.0 m). Fish were fed twice daily for 10 weeks. Growth was significantly reduced with increased soybean inclusion in fish fed both the soybean sources. Independent of the soybean source used, increased inclusion of soybean meal decreased the growth performance. When the substitution level was 45% or more in CSM and 60% in HVS, the specific growth rate (SGR) and the feed efficiency ratio (FER) were significantly lower compared with the control. These results indicated that CSM protein could substitute for 30% fish meal protein, while the substitution level of HVS protein could be 45%, which did not influence the growth of juvenile Japanese seabass. The higher substitution level for HVS compared with CSM was probably due to better nutritional values.  相似文献   

16.
A 9‐week study was conducted to evaluate the potential of processed animal protein (PAP) in comparison with soybean meal (SM) to replace fish meal (FM) in practical diets for European catfish, Silurus glanis, on growth performance, liver transaminase activities and expression of growth‐related genes. Seven isonitrogenous (440 g kg‐1 crude protein) and isoenergetic (16.70 MJ/kg) practical diets were formulated by replacing 0 (control), 30%, 60% and 100% FM with either SM or PAP. Each diet was randomly assigned to triplicate groups of 25 fish per cages fixed in the pond. Fish fed the diet substituted 100% FM from SM or PAP had lower (p < .05) growth performance, feed efficiency and protein retention compared to control and other groups. The decreased growth performance occurred concomitant with a decline in the expression level of liver GHR/IGF‐I axis genes. The gene expression and activity of liver protein metabolism enzymes also positively correlated with growth performance. Findings of this study indicated that both SM and PAP were equally effective in replacing FM up to 60% in the practical diet of Silurus glanis. Total replacement of FM either with SM or PAP induced negative influences on growth and feed utilization.  相似文献   

17.
The value of defatted soybean meal as a protein source for sea bream fingerlings (15.2±4.4 g on average) growing to market size (300–350 g) was evaluated by feeding extruded isonitrogenous and isoenergetic diets (46% protein and 22 MJ kg−1) containing 20%, 30%, 40% and 50% soybean meal considering two phases. On day 87, the fish weight ranged between 66 and 81 g. The specific growth rate (SGR) of sea bream fed 50% soybean was lower (1.73% day−1) than that of fish fed 20% (1.87% day−1) and 30% (1.93% day−1), but the food conversion ratio (FCR) was not significantly affected, and a quadratic significant trend was observed for the feed intake (FI) in relation to the dietary soybean level. At the end of the second phase on day 309, fish weight was between 303 and 349 g, but SGR and FCR were similar for all diets, and ranged between 0.64 and 0.69% day−1, and 1.95 and 2.10% day−1 respectively. The final biometric parameters were not affected by the diets, although the levels of some free amino acids in the muscle were affected. Sensory differences were detected by panellists in fish fed diet 20% as compared those fed diet 50%, which had a less marine flavour and was less juicy. The global growth results suggest the possibility of feeding sea bream weighing less than 80 g with 30% soybean meal, and for fish weighing more than 80 g, a 50% dietary soybean meal can be used until the fish reach commercial weight, with no negative effects on growth or feed efficiency. Nevertheless, when sensory analysis and economic aspects are considered, the maximum inclusion level of soybean was 20–22%.  相似文献   

18.
This study evaluated various by‐catch and by‐product meals of marine origin with red drum (Sciaenops ocellatus L.). Four different kinds of by‐catch or by‐product meals [shrimp by‐catch meal from shrimp trawling, Pacific white shrimp (Litopenaeus vannamei (Boone)) processing waste meal, red salmon (Oncorhynchus nerka (Walbaum)) head meal, and Pacific whiting (Merluccius productus (Ayres)) meal] were substituted for Special Select? menhaden fish meal at 33% or 67% of crude protein in diets formulated to contain 40% crude protein, 12% lipid, and 14.6 kJ digestible energy g?1. Each of these diets and three additional diets consisting of shrimp processing waste meal formulated on a digestible‐protein basis and two Pacific whiting diets containing reduced levels of ash were also evaluated in two 6‐week feeding trials with juvenile red drum (initial weight of 4–5 and 1–2 g fish?1 in trials 1 and 2). Red drum fed by‐catch meal at either level of substitution performed as well as fish fed the control diet; whereas, fish fed shrimp processing waste meal diets had significantly (P≤0.05) reduced weight gain and feed efficiency ratio values compared with the controls, even when fed on a digestible‐protein basis. The diets containing Pacific whiting at either levels of substitution and regardless of ash level supported similar performance of red drum as those fed the control diet. Fish fed the red salmon head meal diet fared poorly, probably owing to an excessive amount of lipid in the diet that became rancid. Overall, by‐catch meal associated with shrimp trawling and Pacific whiting appear to be suitable protein feedstuffs for red drum.  相似文献   

19.
A total of six isoprotein and isolipid diets for Atlantic salmon, Salmo salar L., were prepared substituting from 0 to 100% of fish meal protein (0–68% of diet by dry weight) with meal from Antarctic krill (Euphausia superba). The feed produced from high inclusion levels of krill meal had lower ability to absorb lipid during vacuum coating than fish meal. Both amino acid and fatty acid compositions of the diets were fairly similar. The experiment commenced using salmon averaging 500 g and ended at a mean weight of 1500–1800 g (140 days of feeding). Moderate amounts of krill meal (20–60% of krill protein) in the diets increased growth during the first 71 days of feeding compared with the fish meal control, while no growth difference was observed during the last 69 days of feeding. This may, at least in parts, be explained by a feed‐attractant function of the krill meal. Muscle dry weight and lipid concentrations were unaffected by the diet. Feed conversion rate increased with high levels of krill meal in the diets (e.g. for the last period from 0.94 in the 0% diet to 1.26 in the 100% diet). This indicates that the fish were able to compensate by eating more to maintain growth. The apparent digestibility coefficients of dry matter and protein were not influenced by diet, but both faecal moisture and lipid had a tendency to increase at the highest inclusion level (all protein from krill meal). This may be related to chitin in the krill diet that is known to decrease lipid absorption and induce diarrhoea (increased water content in faeces). Chitin was not utilized to any major extent. Welfare parameters such as blood haemoglobin, red blood cell counts, plasma protein, cholesterol, triacylglycerols and glucose levels were unaffected by diets. Clinical indicators of cellular damage (alanine aminotransferase and aspartate aminotransferase) were similar indicating no diet‐induced tissue damage during the trial.  相似文献   

20.
Diets, containing dehulled, solvent-extracted soybean meal in amounts corresponding to 0. 20 and 40% soybean protein of total protein, were fed for 10 months to duplicate groups of Atlantic salmon, Salmo salar L., weighing about 900 g, held in sea water. Fish fed the diet containing 20% soybean protein grew as well as fish fed the diet with high-quality fish meal as the sole protein source, whilst the fish fed the diet containing 40% soybean protein grew significantly less. No significant differences were found for dressing percentage, condition factor or relative liver weight between the dietary treatments. Fish fed the control or the 20% soybean meal (SBM) diets were not significantly different in body fat content, whilst this was significantly lower in the fish fed the 40% SBM diet. There were no significant differences between the dietary treatments in body protein content. Inclusion of SBM seemed to affect neither carcass calcium, carcass zinc, nor plasma calcium, magnesium or potassium. The plasma zinc content increased and plasma iron decreased by inclusion of SBM in the diets, probably reflecting dietary contents of zinc and iron. Liver iron was not affected. Plasma phosphate was significantly decreased by inclusion of SBM in the diet, whilst plasma glucose, total protein and haemoglobin were not affected. For plasma free amino acids, the only significant differences were a decrease in taurine with increasing SBM inclusion and methionine was significantly higher for the SBM groups than for the control groups.  相似文献   

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