间接免疫荧光显微术检测泡桐丛枝病原MLO的研究

以感染类菌原体ＭＬＯ的泡桐组培苗为病原繁殖材料，采用差速离心和Ｐｅｒｃｏｌｌ不连续密度梯度离心来提纯ＭＬＯ作为免疫抗原制备兔抗血清。抗血清经健康汁液吸收后，作为第一抗体，用异硫代氰酸荧光素ＦＩＦＣ标记的羊抗兔免疫球蛋白作为第二抗体，进行间接免疫荧光显微镜检查染病组织中的ＭＬＯ特异性荧光。经与ＤＡＰＩ染色技术比较，找出了适宜的徒手切片和减少非特异性反应的途径。此技术具有灵敏度高、特异性强和测定简便等特点。适用于ＭＬＯ组织定位、组培苗脱毒效果评估、病原株系鉴定和病害检疫等。     

泡桐丛枝病研究新进展

介绍了近年来泡桐丛枝病研究取得的一些进展,主要包括病原类菌原体(MLO)的检测和鉴定、病原致病机理、病原在树体内的分布和季节变动规律、寄主抗病性鉴定、治疗药剂的筛选等方面的最新研究结果和动向。并且根据新提出的泡桐丛枝病原MLO浸染循环模式,探讨了我国发生泡桐丛枝病流行的关键因子,以及今后防治的策略问题。     

注射传播泡桐丛枝病类菌原体的初步研究

以泡桐丛枝病类菌原体的敏感指示植物长春花苗为材料,用叶柄注射法注入感病泡桐叶汁液进行接种试验,结果表明,有1/4供试植株感病,其症状与泡桐丛枝病相一致,将感病植株叶脉切片镜检,发现有类菌原体,其形态、大小及结构,均与泡桐丛枝病类菌原体相同。为该病病原的传播及回接提供了一条简便的途径。     

枣树脱除MLO组织培养技术概述

介绍枣树脱除MLO的理论依据,茎尖组织培养和热处理后茎尖培养2种常见脱除MLO的组织培养技术,阐述症状观察法、电子显微镜法、荧光染色等几种枣树脱毒苗检测方法的特点.     

水竹丛枝病的研究——Ⅰ.症状和病原

对水竹丛枝病的症状和病原进行观察研究表明:水竹丛枝病的典型症状是病枝节间缩短,侧枝、细枝丛生。每年从4月起,在病枝叶鞘内产生白色米粒状子座,5月中旬开始在其外围产生褐色垫状子座:即瘤座菌的有性世代;通过电镜观察,发现在病株同一组织的超薄切片中,同时存在类细菌,类菌原体和真菌菌丝,其中真菌菌丝的形态和大小与病株叶鞘内的瘤座菌子座切片菌丝相似。用分离培养的类细菌培养液针注接种能诱发致病,其症状与自然发病相同。进行再分离,其菌落形态、菌体大小和形态结构均与接种物相似。     

Intensive observations on the effect of some insecticides on Nephotettix virescens (Distant), a vector of virus and MLO diseases,with special reference to its transmission ability

The effects of some insecticides on Nephotettix virescens (Distant), a leafhopper vector of virus and MLO diseases, were studied with particular reference to their efficacy in affecting vector behaviour and transmission of rice tungro virus (RTV). When 10- and 15-day old seedlings from carbofuran-treated seeds were exposed to viruliferous vectors in cages, the percentages of transmission were 40 and 50, respectively against cent per cent in the control. Spraying of EC formulations of dimethoate, phosphamidon and methamidophos was not effective but decemethrin showed spectacular knockdown effect and gave absolute protection against RTV transmission upto 7 days. Since the transmission threshold of RTV is considerably shorter than of mycoplasmal diseases, the treatments affecting RTV transmission may reasonably be expected to be quite effective against MLO diseases like rice yellow dwarf and sandal spike, transmitted by N. virescens.     

The structural and chemical characteristics of the pericarp are important in Tilia miqueliana seed dormancy

New Forests - Pericarp structure and permeability in Tilia miqueliana were explored using anatomy, paraffin section detection, scanning electron microscopy, and magnetic resonance imaging. The...     

泡桐抗丛枝病相关因素的分析

通过多元线性分析,通径分析及逐步回分析表明,在形态特征,组织机械,生化物质,传毒昆虫因素中,茶翅蝽对泡桐丛枝病发病率高低的影响最显著,是泡桐丛枝闰闰原菌（MLO）的主要传毒昆虫,从而指出了在选择泡桐抗丛枝病优良天性系的过程中,应注重选择叶背多毛,叶片厚实且单宁含量高的泡桐种类。