Estimation of mycorrhizal colonization of the roots of oak seedlings inoculated with an ectomycorrhizal fungus,Laccaria amethystea

Six-month-old seedlings of Quercus serrata and Quercus glauca in a nursery were inoculated with the ectomycorrhizal fungus Laccaria amethystea encapsulated in alginate gel and grown in the nursery. The seedlings were collected at 1, 3, and 5 months after the inoculation and examined for colonization of the root system with ectomycorrhizal fungi. The roots within 5 months after the inoculation showed rudimentary ectomycorrhizal colonization. The level of colonization of the root system was estimated based on the intensity of hyphal covering on the root tips by staining with a fluorescent dye and expressed as an index of mycorrhizal colonization (IMC). IMC increased with the time after inoculation and reached values of 4 and 12% in Q. serrata and Q. glauca, respectively at 5 months after the inoculation. The determination of IMC may enable to assess the development of mycorrhizal colonization of the root system that shows rudimentary ectomycorrhizas after the inoculation.     

The influence of inorganic nutrient fertilization on the growth,nutrient composition and vesicular-arbuscular mycorrhizal colonization of pretransplant rice (Oryza sativa L.) plants

Summary The effects of P, N and Ca+Mg fertilization on biomass production, leaf area, root length, vesiculararbuscular mycorrhizal (VAM) colonization, and shoot and root nutrient concentrations of pretransplant rice (Oryza sativa L.) plants were investigated. Mycorrhizal plants generally had a higher biomass and P, N, K, Ca, Mn, Fe, Cu, Na, B, Zn, Al, Mg, and S shoot-tissue nutrient concentrations than non-mycorrhizal plants. Although mycorrhizal plants always had higher root-tissue nutrient concentrations than non-mycorrhizal plants, they were not significantly different, except for Mn. N fertilization stimulated colonization of the root system (colonized root length), and increased biomass production and nutrient concentrations of mycorrhizal plants. Biomass increases due to N were larger when the plants were not fertilized with additional P. P fertilization reduced the colonized root length and biomass production of mycorrhizal plants. The base treatment (Ca+Mg) did not significantly affect biomass production but increased the colonized root length. These results stress the importance of evaluating the VAM rice symbiosis under various fertilization regimes. The results of this study suggest that pretransplant mycorrhizal rice plants may have a potential for better field establishment than non-mycorrhizal plants.     

Uptake of an amino acid by ectomycorrhizal fungi in a boreal forest

We assessed the degree to which ectomycorrhizal fungi exploit organic nitrogen in situ. In an Alaskan boreal forest, we identified pairs of sporocarps from five taxa of ectomycorrhizal fungi. We added ¹³C-labeled alanine to the soil surrounding one sporocarp within each pair; the second served as an unlabeled control. Peak rates of ¹³C-respiration from alanine were higher in the labeled sporocarp plots than the controls, indicating that the ¹³C-alanine was detectably respired from the soil. “Reference” plots adjacent to the sporocarps served as an indication of background ¹³C-respiration rates released by the soil community as a whole. Ectomycorrhizal sporocarps displayed higher ¹³C-respiration rates than their reference plots. Thus, the sporocarps and associated mycorrhizal mycelium appeared to contribute significantly to the release of alanine-derived ¹³CO₂, confirming the hypothesis that ectomycorrhizal fungi may access soil amino acid pools under natural conditions.     

Influence of bacteria on growth and phosphorus nutrition of mycorrhizal corn

Com plants were grown in a non‐sterile soil in a greenhouse or in hydroponic culture in a growth chamber. We studied the influence of chitinolytic, pectinolytic, P‐solubilizing bacterial isolates, and a collection of bacterial strains on the development of native vesicular‐arbuscular mycorrhizal (VAM) populations, colonization of roots by the VAM fungus Glomus fasciculatum and their influence on the phosphorus (P) nutrition and growth of plants. As compared with VAM native control, the most potent stimulants for root colonization of soil‐grown plants by the VAM native population was a strain of Agrobacterium radiobacter and isolate H30. All bacteria used significantly supressed shoot fresh weight of mycorrhizal plants (‐13% up to ‐37%), with the exception of Agrobacterium. Under hydroponic conditions, the P‐solubilizing isolate F27 significantly stimulated the intensity of mycorrhiza, the number of arbuscules in roots, and increased both the P concentration and P content in corn shoots (+30% and +35%), than did the VAM fungus alone. Isolate F27 significantly increased shoot dry weight as compared with the mycorrhizal control. The other bacteria did not influence biomass production of corn.     

Infection of valuable broadleaf hardwood trees by Glomus mosseae: growth and mineral nutrition

In the past century, the excessive exploitation of the environment by human beings has resulted in the depletion of valuable broadleaf hardwood trees in Italian forests, creating a need for re-forestation. The aim of this research was to verify whether a vescicular-arbuscular mycorrhizal (VAM) fungus is able to colonise the root of valuable hardwood trees and to evaluate the impact of the VAM fungus on growth and macroelement nutrition of its plant hosts.Four species of valuable broadleaf hardwood trees, Prunus avium L., Fraxinus excelsior L., Acer pseudoplatanus L., and Juglans nigra L., were inoculated with Glomus mosseae, a VAM fungus, and cultivated in a greenhouse. Infection after inoculation and root colonization by the fungus, tree growth, and macro-element nutrition were evaluated two-years after inoculation. G. mosseae formed mycorrhizae on all plants. However, different morphological aspects - predominantly the formation of Arum type arbuscles in P. avium and F. excelsior - were observed. A general improvement of macro-element nutrition from species to species characterised an enhanced growth of mycorrhizal plants. Therefore, it is plausible that the association of VAMs with these broadleaf trees, could overcome the difficulties encountered in the transplanting and the slow growth typical of these tree species.Although numerous articles have reported the beneficial effects of ectomycorrhizal fungi on trees, there is a sparse literature on the association of VAM with tree species. Therefore, this study contributes to the understanding of the role of the symbiosis between valuable broadleaf trees and VAM fungi in macroelement nutrition.     

Rhizodeposition and net release of soluble organic compounds by pine and beech seedlings inoculated with rhizobacteria and ectomycorrhizal fungi

Summary A lysimetric experiment was performed in a greenhouse to evalute root deposition and net release of soluble organic compounds after 1 and 2 years from pine and beech seedlings inoculated with an ectomycorrhizal fungus (Laccaria laccata) and/or rhizobacteria (Agrobacterium radiobacter for beech and Agrobacterium sp. for pine). Total C compounds released in the rhizosphere of both plants increased after inoculation with the bacteria or ectomycorrhizal fungus. The rhizobacteria increased root and plant growth and rhizodeposition, but the mycorrhizal fungi appeared to increase only root deposition. Soluble C compounds, collected after 2 years, represented only 0.1–0.3% of the total C compounds released into the rhizosphere, and were modified by inoculation with the microorganisms. After inoculation with the bacteria, levels of sugars and amino acids decreased in pine and beech rhizospheres, whereas organic acids increased, especially in the pine rhizosphere. In the rhizosphere of mycorrhizal beeches, sugar and amino acids increased, and organic acids differed from those released from non-mycorrhizal beeches. In the mycorrhizal pine rhizosphere, however, all compounds decreased. Following dual inoculations, mycorrhizal colonization increased, no effect on plant growth was observed, and virtually no organic acids were detected.     

Response of two species of cassia and Gliricidia sepium to vesicular‐arbuscular mycorrhizal infection

Abstract

A pot experiment was conducted to evaluate the response to vesicular‐arbuscular mycorrhizal (VAM) infection of two species of Cassia and Gliricidia sepium in an oxisol with two levels of soil P. At P level optimum for VAM activity, comparable levels of VAM infection were observed on the roots of the test species. However, the species reacted differently to VAM infection. Hence, VAM inoculation significantly stimulated dry matter accumulation in C. spectabilis and G. sepium, but had no positive influence on this variable in C. reticulata. These differences were not explainable in terms of tissue P status, because P uptake was significantly enhanced by inoculation in all the species tested. At the higher soil P level, roots of all species were colonized by the inoculum, but colonization level in C. reticulata and G. sepium was reduced. Nevertheless, only C. spectabilis benefitted from the presence VAM endophytes at this level of soil P. The results underline the significance of ascertaining the mycorrhizal requirements of host species before reliable VAM inoculation recommendation could be made.     

Effects of substrate sterilization,fungicide treatment,and mycorrhization helper bacteria on ectomycorrhizal formation of pedunculate oak (Quercus robur) inoculated with Laccaria laccata in two peat bare-root nurseries

Summary Pedunculate oak seedlings (Quercus robur) inoculated with the ectomycorrhizal fungus Laccaria lacata were grown for 1 year on fertilized sphagnum peat in two nurseries. Three factors affecting microbial populations in the substrate were studied, fungicide treatment of the seeds, peat disinfection before sowing (methyl bromide or steam pasteurization), and inoculation with mycorrhization helper bacteria. Treatment of acorns with Iprodione had no depressive effect on mycorrhiza formation. Both disinfection techniques were equivalent, stimulating or depressing mycorrhiza formation depending on the initial microflora in the peat. The introduction of two previously selected mycorrhization helper bacteria (one Pseudomonas fluorescens and one unidentified fluorescent pseudomonad), isolated from L. laccata sporocarps associated with Douglas fir—L. laccata ectomycorrhizas in other nurseries, significantly increased the mycorrhizal rate from 30 to 53% of the short roots. The implications of these results for the controlled mycorrhization of planting stocks and the specificity of mycorrhization helper bacteria are discussed.     

Plant and soil responses to mycorrhizal fungi and rhizobacteria in nodulated or nitrate-fertilized peas (Pisum sativum L.)

Rhizosphere organisms affect plant development and soil stability. This study was conducted to determine the effects of a vesicular-arbuscular mycorrhizal (VAM) fungus [Glomus mosseae (Nicol. &>; Gerd.) Gerd. and Trappe] and a rhizobacterium (Bacillus sp.) on nitrate-fertilized or nodulated pea (Pisum sativum L.) plants and on the status of water-stable soil aggregates. The plants were grown in pots in a yellow clay-loam soil, and inoculated with the VAM fungus and the rhizobacterium, with one of the two, or with neither. The Bacillus sp. and G. mosseae did not affect shoot dry mass in nodulated plants. Under N fertilization, the VAM fungus enhanced plant growth, while the rhizobacterium inhibited shoot growth, VAM root colonization, and nodule formation, but enhanced the root:shoot and the seed:shoot ratios. The inhibition of shoot growth and of root colonization appeared to be related. The water stability and pH of the VAM soils were higher than those of the non-VAM soils. The rhizobacterium enhanced the water-stable aggregate status in the non-VAM soils only. Under both N-nutrition regimes, the soils had the greatest proportion of the water-stable aggregates when inoculated with both rhizo-organisms and the lowest when colonized by neither. The two rhizo-organisms affected both plants and soil, and these effects were modified by the source of N input through N₂ fixation or fertilization. Received: 5 April 1995     

Dual inoculation of pretransplant stage Oryza sativa L. plants with indigenous vesicular-arbuscular mycorrhizal fungi and fluorescent Pseudomonas spp.

Summary This study examined the response of rice (Oryza sativa L.) plants at the pretransplant/nursery stage to inoculation with vesicular-arbuscular mycorrhizal (VAM) fungi and fluorescent Pseudomonas spp., singly or in combination. The VAM fungi and fluorescent Pseudomonas spp. were isolated from the rhizosphere of rice plants. In the plants grown in soil inoculated with fluorescent Pseudomonas spp. alone, I found increases in shoot growth, and in root length and fine roots, and decreases in root growth, and P and N concentrations. In contrast, in the plants colonized by VAM fungi alone, the results were the reverse of those of the pseudomonad treatment. Dual inoculation of soil with VAM fungi and fluorescent Pseudomonas spp. yielded plants with the highest biomass and nutrient acquisition. In contrast, the plants of the control treatment had the lowest biomass and nutrient levels. The dual-inoculated plants had intermediate root and specific root lengths. The precentages of mycorrhizal colonization and colonized root lengths were significantly lower in the dual-inoculated treatment than the VAM fungal treatment. Inoculation of plants with fluorescent Pseudomonas spp. suppressed VAM fungal colonization and apparently reduced photosynthate loss to the mycorrhizal associates, which led to greater biomass and nutrient levels in dual-inoculated plants compared with plants inoculated with VAM fungi alone. Dual inoculation of seedlings with fluorescent Pseudomonas spp. and VAM fungi may be preferable to inoculation with VAM alone and may contribute to the successful establishment of these plants in the field.     

Mycorrhizal dependency of two hawaiian endemic tree species: Koa and mamane 1

Vesicular‐arbuscular mycorrhizal (VAM) fungi have been proposed as a low‐input solution to the problem of inadequate phosphorus (P) levels in many tropical and subtropical soils. To determine the mycorrhizal dependency of two Hawaiian endemic tree species, mamane (Sophora chrysophylla Seem.) and koa (Acacia koa Gray), seedlings were grown in the greenhouse with and without the VAM fungus, Glomus aggregatum Schenck and Smith emend Koske, at three levels of soil solution P (0,0.02, and 0.20 mg P/L) in a volcanic ash soil. Inoculation significantly increased colonization of roots by the VAM fungus in both mamane and koa seedlings. At 0.02 mg P/L, mamane inoculated with the VAM fungus had significantly greater subleaflet P concentrations at 48 days after planting (DAP), and significantly greater leaf areas, shoot dry weights, and root lengths at harvest compared to uninoculated plants. At 0 mg P/L, koa grown in association with the VAM fungus had significantly greater subleaflet P concentrations at 41 DAP, and significantly greater leaf areas, and dry weights of leaves, stems, and roots at harvest. Mamane was highly dependent on the VAM association for maximum growth, while koa was moderately dependent on the VAM association. These results demonstrate that P uptake and early growth of mamane and koa can be increased significantly at low soil P levels by inoculating seedlings with an effective VAM fungus. Future research needs to demonstrate continuing positive growth benefits of VAM fungal inoculation after transplanting from the nursery to field conditions.     

The sensitivity of three vesicular‐arbuscular mycorrhizal species to simulated erosion

Abstract

A greenhouse experiment was conducted to determine the effectiveness of three vesicular‐arbuscular mycorrhizal (VAM) species in an Oxisol subjected to simulated erosion using Leucaena leucocephala as an indicator host. The extent of colonization of leucaena roots increased significantly due to VAM inoculation of the eroded and uneroded soils. The highest level of VAM colonization was observed when leucaena was grown in association with Glomus aggregatum. followed by G. mosseae and G. etunicatum. Increased infection associated with inoculation of the eroded soil did not result in enhanced mycorrhizal effectiveness. Inoculation of the uneroded soil, however, led to significant improvement in root colonization as well as in symbiotic effectiveness. Suppression in expression of mycorrhizal effectiveness in the eroded soil appears to be a result of nutrient deficiency. The results suggest the importance of restoring lost nutrients before differences in VAM species could be effectively exploited for a successful establishment of a mycorrhizal plants in eroded soils.     

Vesicular-arbuscular mycorrhizae in tropical sedges of southern India

Twenty-four species of sedges (representing six genera) from different vegetation types in Western Ghats, South India, were examined for vesicular-arbuscular mycorrhizal (VAM) associations. All the sedges had VAM fungal infections with hyphae and vesicles, but arbuscules were observed only in 42% of the total species. The VAM fungal colonization varied considerably between species, ranging from 9 to 62%. Root colonization was positively and negatively correlated with root diameter and root hair length, respectively. The number of VAM fungal spores in the rhizosphere varied from 5 to 86 g^-1 soil. No significant relationship was found between spore numbers or root colonization and either soil pH or moisture. Four Glomus spp., one Acaulospora sp., one Sclerocystis spp., and one Gigaspora sp. were identified among the VAM fungal spores. The results reflect a high incidence of VAM in sedges occurring in Western Ghats and emphasize the need to assess the VAM status of plant species from different ecosystems to understand their mycorrhizal status.     

External phosphorus requirement of mycorrhizal and non-mycorrhizal barley and soybean plants

An experiment was conducted under greenhouse conditions to evaluate the effects of vesicular arbuscular mycorrhizal (VAM) fungi on the external P requirements of barley and soybeans. The plants were grown in pots containing a P-deficient soil. A range of 10 P levels was obtained by adding 0, 20, 30, 40, 50, 60, 70, 110, 160, or 310 mg P kg^-1 as NaH₂PO₄ 2H₂O. Half of the pots were inoculated with the VAM fungus Glomus intraradices. The P concentration in the soil solution was determined using an adsorption isotherm and plotted against the relative yield. Barley did not respond to mycorrhizal inoculation and we concluded that P nutrition was not the limiting factor on the growth of this lowmycotrophic plant. In contrast, mycorrhizal inoculation stimulated the growth of soybeans. The external P requirements were 0.110 g ml^-1 for mycorrhizal and 0.148 g ml^-1 for non-mycorrhizal soybeans to obtain 80% of the maximum yield. In terms of P fertilization this corresponds to a saving of 222 kg P₂O₅ ha^-1. The mycorrhizal dependency of the soybean was highly correlated with the P concentration in the soil solution and it is proposed that both values should be displayed together.     

Influence of Vesicular Arbuscular Mycorrhizal Fungi and Applied Phosphorus on Root Colonization in Wheat and Plant Nutrient Dynamics in a Phosphorus-Deficient Acid Alfisol of Western Himalayas

Vesicular arbuscular mycorrhizal (VAM) fungi symbiosis confers benefits directly to the host plant's growth and yield through acquisition of phosphorus and other macro- and micronutrients, especially from phosphorus (P)–deficient acidic soils. The inoculation of three VAM cultures [viz., local culture (Glomus mosseae), VAM culture from Indian Agricultural Research Institute (IARI), New Delhi (Glomus mosseae), and a culture from the Centre for Mycorrhizal Research, Energy Research Institute (TERI), New Delhi (Glomus intraradices)] along with P fertilization in wheat in a P-deficient acidic alfisol improved the root colonization by 16–24% while grain and straw yields increased by 12.6–15.7% and 13.4–15.4%, respectively, over the control. Uptake of nitrogen (N), P, potassium (K), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) was also improved with VAM inoculation over control, but the magnitude of uptake was significantly greater only in the cases of P, Fe, Zn, and Cu. Inoculation of wheat with three VAM cultures in combination with increasing inorganic P application from 50% to 75% of the recommended P₂O₅ dose to wheat through the targeted yield concept following the soil-test crop response (STCR) precision model resulted in consistent and significant improvement in grain and straw yield, macronutrient (NPK) uptake, and micronutrient (Fe, Mn, Zn, Cu) uptake in wheat though root colonization did not improve at P₂O₅ doses beyond 50% of the recommended dose. The VAM cultures alone or in combination with increasing P levels from 50% to 75% P₂O₅ dose resulted in reduction of diethylenetriaminepentaacetic acid (DTPA)–extractable micronutrient (Fe, Mn, Zn, Cu) contents in P-deficient acidic soil over the control and initial fertility status, although micronutrient contents were relatively greater in VAM-supplied plots alone or in combination with 50% to 75% P₂O₅ dose over sole application of 100% P₂O₅ dose, thereby indicating the positive role of VAM in nutrient mobilization and nutrient dynamics in the soil–plant system. There was significant improvement in available N and P status in soil with VAM inoculation coupled with increasing P levels upto 75% P₂O₅ dose, although the greatest P buildup was obtained with sole application of 100% P₂O₅ dose. The TERI VAM culture (Glomus intraradices) showed its superiority over the other two cultures (Glomus mosseae) in terms of crop yield and nutrient uptake in wheat though the differences were nonsignificant among the VAM cultures alone or at each P level. Overall, it was inferred that use of VA-mycorrhizal fungi is beneficial under low soil P or in low input (nutrient)–intensive agroecosystems.     

Boron fertilization,vesicular‐arbuscular mycorrhizal colonization and growth of citrus jambhiri lush

The role of boron (B) fertilization in the vesicular‐arbuscular mycorrhizal (VAM) colonization and growth of container‐grown Citrus seedlings was evaluated. Citrus jambhiri Lush, seedlings inoculated with Glomus fasciculatum, Glomus etunicatum or non‐inoculated were grown in a sandy loam soil for sixteen weeks. Seedlings were fertilized with 25 ug/ml B applied to the foliage as a mist, to the soil as a solution, or to both the foliage and the soil. Boron applied to the foliage or to the soil significantly increased root exudation of reducing sugars and amino acids two weeks after seedling germination. Subsequently, foliar fertilization with B significantly increased VAH colonization of seedlings relative to the controls. Plants inoculated with G. fasciculatum were larger and had greater VAM development than those inoculated with G. etunicatum. The growth of the noninoculated seedlings was not enhanced by B fertilization. The superior VAH colonization and growth of inoculated seedlings fertilized with B suggests that B stimulates the efficacy of plant fungi symbiosis.     

Response of pineapple to mycorrhizal inoculation and fosetyl‐Al treatment

Abstract

A pot experiment was conducted in the greenhouse to determine the influence of vesicular‐arbuscular mycorrhizal (VAM) inoculation on growth of pineapple (Ananas comosus, cv.’Smooth Cayenne') and its interaction with fosetyl‐Al in a Wahiawa soil (Tropeptic Eutrustox) at soil solution P levels of 0.003, 0.02 and 0.2 mg/L. Pineapple crowns were dipped in a solution of fosetyl‐Al before planting. Inoculation of soil with the fungus Glomus aggregatum (Schenck & Smith emend. Koske) significantly increased VAM colonization of pineapple roots at soil solution P levels of 0.003 and 0.02 mg/L. VAM inoculation also increased mycorrhizal effectiveness measured six weeks after planting. At harvest, pineapple grown in the inoculated soil at the lowest P level had significantly higher D‐leaf P concentration and plant fresh weight than that grown in the uninoculated soil. Fosetyl‐Al appears to have no significant effect on VAM‐pineapple interaction.     

Inoculation with phosphate-solubilizing microorganisms and a vesicular-arbuscular mycorrhizal fungus improves dry matter yield and nutrient uptake by wheat grown in a sandy soil

 The effect of inoculating wheat (Triticum aestivum L.) with the PO₄ ^3–-solubilizing microorganisms (PSM) Bacillus circulans and Cladosporium herbarum and the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus sp. 88 with or without Mussoorie rock phosphate (MRP) amendment in a nutrient-deficient natural sandy soil was studied. In the sandy soil of low fertility root colonization by VAM fungi was low. Inoculation with Glomus sp. 88 improved root colonization. At maturity, grain and straw yields as well as N and P uptake improved significantly following inoculation with PSM or the VAM fungus. These increases were higher on combined inoculation of PSM and the VAM fungus with MRP amendment. In general, a larger population of PSM was maintained in the rhizosphere of wheat in treatments with VAM fungal inoculation and MRP amendment. The results suggest that combined inoculation with PSM and a VAM fungus along with MRP amendment can improve crop yields in nutrient-deficient soils. Received: 4 September 1997     

Use of nutrient: phosphorus ratios to evaluate the effects of vesicular arbuscular mycorrhiza on nutrient uptake in unsterilized soils

Summary Red clover was grown in soil previously treated with P at various rates, and growth, nutrient uptake, nutrient uptake in relation to phosphorus values, and levels of vesicular-arbuscular mycorrhizal (VAM) infection were determined. The soil was a silty clay loam and Glomus lacteum was the only fungus colonizing the plant roots. An examination of the effects of various rates of P application and of VAM colonization on nutrient (P, K, Ca, Mg, Mn, Fe, and Zn) uptake showed that the Mg : P ratio significantly increased and the Mn : P ratio significantly decreased with increasing VAM infection. It is concluded that in the Trifolium pratense-Glomus lacteum symbiosis, mycorrhizae improve Mg uptake and depress Mn uptake.     

Interactions between mycorrhizal fungi and mycorrhizosphere bacteria during mineral weathering: Budget analysis and bacterial quantification

The impact of ectomycorrhizal fungi or rhizosphere bacteria on tree seedling growth and nutrient uptake is well known. However, few studies have combined those microorganisms in one experiment to clarify their relative contribution and interactions in nutrient acquisition. Here, we monitored the respective contributions of pine roots, two ubiquitous forest ectomycorrhizal fungi Scleroderma citrinum and Laccaria bicolor, and two S. citrinum-mycorrhizosphere bacterial strains of Burkholderia glathei and Collimonas sp., on mineral weathering, nutrient uptake, and plant growth. Pinus sylvestris plants were grown on quartz–biotite substrate and inoculated or not with combinations of mycorrhizal fungi and/or bacterial strains. Magnesium and potassium fluxes were measured and nutrient budgets were calculated. Both ectomycorrhizal fungi significantly increased Mg plant uptake. No significant effects of the two bacterial strains were detected on the K and Mg budgets, but co-inoculating the mycorrhizal fungus S. citrinum and the efficient mineral-weathering B. glathei bacterial strain significantly improved the Mg budget. Similarly, co-inoculating S. citrinum with the Collimonas sp. bacterial strain significantly improved the pine biomass compared to non-inoculated pine plants. In order to better understand this process, we monitored the survival of the inoculated bacterial strains in the quartz–biotite substrate, the pine rhizosphere, and the mycorrhizal niche. The results showed that the two bacterial strains harboured different colonization behaviours both of which depended on the presence of the ectomycorrhizal partner. The populations of the Burkholderia strain were maintained in all these environments with a significantly higher density in the mycorrhizal niche, especially of S. citrinum. In contrast the population of the Collimonas strain reached the detection level except in the treatment inoculated with S. citrinum. These results highlight the need for taking into account the ecology of the microorganisms, and more specifically the fungal–bacterial interactions, when studying mineral weathering and plant nutrition.